Publications

miR-19b regulates hTERT mRNA expression through targeting PITX1 mRNA in melanoma cells.
Sci Rep 2015 Feb 3;5:8201. Epub 2015 Feb 3.
1] Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University, Yonago, Tottori 683-8503, Japan [2] Chromosome Engineering Research Center, Tottori University, Yonago, Tottori 683-8503, Japan.

Highly sensitive luciferase reporter assay using a potent destabilization sequence of calpain 3.
J Biotechnol 2015 Jan 18;194:115-23. Epub 2014 Dec 18.
Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Takamatsu, Kagawa 761-0395, Japan. Electronic address:

A novel and stable mouse artificial chromosome vector.
ACS Synth Biol 2014 Dec 17;3(12):903-14. Epub 2012 Oct 17.
Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University , 86 Nishi-cho, Yonago, Tottori 683-8503, Japan.

Deacetylation of the mitotic checkpoint protein BubR1 at lysine 250 by SIRT2 and subsequent effects on BubR1 degradation during the prometaphase/anaphase transition.
Biochem Biophys Res Commun 2014 Oct 5;453(3):588-94. Epub 2014 Oct 5.
Division of Human Genome Science, Department of Molecular and Cellular Biology, School of Life Sciences, Faculty of Medicine, Tottori University, 86 Nishi-cho, Yonago, Tottori 683-8503, Japan; Chromosome Engineering Research Center, Tottori University, 86 Nishi-cho, Yonago, Tottori 683-8503, Japan. Electronic address:

Down syndrome-associated haematopoiesis abnormalities created by chromosome transfer and genome editing technologies.
Sci Rep 2014 Aug 27;4:6136. Epub 2014 Aug 27.
1] Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University, 86 Nishi-cho, Yonago, Tottori 683-8503, Japan; Tel: +81-859-38-6219 [2] Chromosome Engineering Research Center (CERC), Tottori University, 86 Nishi-cho, Yonago, Tottori 683-8503, Japan.


A novel transchromosomic system: stable maintenance of an engineered Mb-sized human genomic fragment translocated to a mouse chromosome terminal region.
Transgenic Res 2014 Jun 2;23(3):441-53. Epub 2014 Feb 2.
Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University, 86 Nishi-cho, Yonago, Tottori, 683-8503, Japan.

PITX1 is a reliable biomarker for predicting prognosis in patients with oral epithelial dysplasia.
Oncol Lett 2014 Mar 24;7(3):750-754. Epub 2013 Dec 24.
Division of Molecular Genetics and Biofunction, Institute of Regenerative Medicine and Biofunction, Tottori University Graduate School of Medical Science, Yonago, Tottori 683-8503, Japan.

Bi-HAC vector system toward gene and cell therapy.
ACS Synth Biol 2014 Feb 9;3(2):83-90. Epub 2014 Jan 9.
Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Sciences, Tottori University , 86 Nishi-cho, Yonago, Tottori 683-8503, Japan.

Highly stable maintenance of a mouse artificial chromosome in human cells and mice.
Biochem Biophys Res Commun 2013 Dec 9;442(1-2):44-50. Epub 2013 Nov 9.
Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University, 86 Nishi-cho, Yonago, Tottori 683-8503, Japan; Chromosome Engineering Research Center, Tottori University, 86 Nishi-cho, Yonago, Tottori 683-8503, Japan.

The transfer of human artificial chromosomes via cryopreserved microcells.
Cytotechnology 2013 Oct 15;65(5):803-9. Epub 2013 Mar 15.
Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University, 86 Nishi-cho, Yonago, Tottori, 683-8503, Japan.

An induced pluripotent stem cell-mediated and integration-free factor VIII expression system.
Biochem Biophys Res Commun 2013 Feb 3;431(2):336-41. Epub 2013 Jan 3.
Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University, Tottori, Japan.

Trans-chromosomic mice containing a human CYP3A cluster for prediction of xenobiotic metabolism in humans.
Hum Mol Genet 2013 Feb 2;22(3):578-92. Epub 2012 Nov 2.
Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University, 86 Nishi-cho, Yonago, Tottori 683-8503, Japan.


Prognostic significance of sirtuin 2 protein nuclear localization in glioma: an immunohistochemical study.
Oncol Rep 2012 Sep 19;28(3):923-30. Epub 2012 Jun 19.
Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University, and Department of Surgical Pathology, Toyama University Hospital, Yonago, Tottori 683-8503, Japan.


Methyl CpG-binding protein isoform MeCP2_e2 is dispensable for Rett syndrome phenotypes but essential for embryo viability and placenta development.
J Biol Chem 2012 Apr 28;287(17):13859-67. Epub 2012 Feb 28.
Department of Mental Retardation and Birth Defect Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Kodaira 187-8502, Japan.



Integration-free and stable expression of FVIII using a human artificial chromosome.
J Hum Genet 2011 Oct 11;56(10):727-33. Epub 2011 Aug 11.
Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University, Yonago, Japan.

Cysteine-rich protein 61 suppresses cell invasion via down-regulation of matrix metalloproteinase-7 expression in the human gastric carcinoma cell line MKN-45.
Mol Med Rep 2010 Jul-Aug;3(4):711-5
Division of Molecular Genetics and Biofunction, Department of Biomedical Science, Tottori University Graduate School of Medical Science, Tottori University, Tottori 683-8503, Japan.



Identification of PITX1 as a TERT suppressor gene located on human chromosome 5.
Mol Cell Biol 2011 Apr 7;31(8):1624-36. Epub 2011 Feb 7.
Department of Biomedical Science, Graduate School of Medical Science, Tottori University, 86 Nishicho, Yonago 683-8503, Japan.

A method for producing transgenic cells using a multi-integrase system on a human artificial chromosome vector.
PLoS One 2011 Feb 24;6(2):e17267. Epub 2011 Feb 24.
Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Sciences, Tottori University, Yonago, Japan.

SIRT2 down-regulation in HeLa can induce p53 accumulation via p38 MAPK activation-dependent p300 decrease, eventually leading to apoptosis.
Genes Cells 2011 Jan 9;16(1):34-45. Epub 2010 Nov 9.
Division of Human Genome Science, Department of Molecular and Cellular Biology, School of Life Sciences, Faculty of Medicine, Tottori University, 86 Nishi-cho, Yonago, Tottori 683-8503, Japan.

Human artificial chromosome with a conditional centromere for gene delivery and gene expression.
DNA Res 2010 Oct 26;17(5):293-301. Epub 2010 Aug 26.
Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Sciences, Tottori University, Nishi-cho, Yonago, Tottori, Japan.

Human chromosome 5 carries a transcriptional regulator of human telomerase reverse transcriptase (hTERT).
Biochem Biophys Res Commun 2010 Aug 16;398(4):695-701. Epub 2010 Jul 16.
Department of Biomedical Science, Graduate School of Medical Science, and Chromosome Engineering Research Center, Tottori University, 86 Nishicho, Yonago 683-8503, Japan.

Development of evaluation system for bioactive substances using human artificial chromosome-mediated osteocalcin gene expression.
J Biochem 2010 Jul 17;148(1):29-34. Epub 2010 Mar 17.
Department of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University, Nishi-cho, Yonago 683-8503, Japan.



Complete genetic correction of ips cells from Duchenne muscular dystrophy.
Mol Ther 2010 Feb 8;18(2):386-93. Epub 2009 Dec 8.
Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University, Yonago, Japan.

Introduction of a normal human chromosome 8 corrects abnormal phenotypes of Werner syndrome cells immortalized by expressing an hTERT gene.
J Radiat Res 2009 May 25;50(3):253-9. Epub 2009 Apr 25.
Radiation Biology Laboratory, Radiation Research Center, Frontier Science Innovation Center, Organization for University-Industry-Government Cooperation, Osaka Prefecture University, Sakai, Osaka, Japan.

SIRT2 downregulation confers resistance to microtubule inhibitors by prolonging chronic mitotic arrest.
Cell Cycle 2009 Apr 19;8(8):1279-91. Epub 2009 Apr 19.
Division of Human Genome Science, Department of Molecular and Cellular Biology, School of Life Sciences, Faculty of Medicine, Tottori University, Yonago, Tottori, Japan.

A noncoding RNA gene on chromosome 10p15.3 may function upstream of hTERT.
BMC Mol Biol 2009 Feb 2;10. Epub 2009 Feb 2.
Division of Pharmacotherapeutics, Department of Pathophysiological and Therapeutic Science, Faculty of Medicine, Tottori University, Yonago, Tottori 683-8503, Japan.

Enhanced apoptosis during early neuronal differentiation in mouse ES cells with autosomal imbalance.
Cell Res 2009 Feb;19(2):247-58
Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University, 86 Nishi-cho, Yonago, Tottori 683-8503, Japan.

A highly stable and nonintegrated human artificial chromosome (HAC) containing the 2.4 Mb entire human dystrophin gene.
Mol Ther 2009 Feb 25;17(2):309-17. Epub 2008 Nov 25.
Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University, Yonago, Japan.

Identification of the human homolog of the imprinted mouse Air non-coding RNA.
Genomics 2008 Dec 2;92(6):464-73. Epub 2008 Oct 2.
Ce-M-M-Research Center for Molecular Medicine of the Austrian Academy of Science, Vienna Biocenter, Dr. Bohr-Gasse 9/4, A1030 Vienna, Austria.



Telomerase-mediated life-span extension of human primary fibroblasts by human artificial chromosome (HAC) vector.
Biochem Biophys Res Commun 2008 May 5;369(3):807-11. Epub 2008 Mar 5.
Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Sciences, Tottori University, 86 Nishimachi, Yonago, Tottori 683-8503, Japan.

Apoptotic pathway induced by transduction of RUNX3 in the human gastric carcinoma cell line MKN-1.
Cancer Sci 2008 Jan 22;99(1):23-30. Epub 2007 Oct 22.
Division of Organ Pathology, Department of Microbiology and Pathology, Faculty of Medicine, Tottori University, Yonago, Tottori 683-8503, Japan.

Production of knockout mice by gene targeting in multipotent germline stem cells.
Dev Biol 2007 Dec 26;312(1):344-52. Epub 2007 Sep 26.
Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Yoshida-Konoe, Sakyo-ku, Kyoto 606-8501, Japan.


MAD1 (mitotic arrest deficiency 1) is a candidate for a tumor suppressor gene in human stomach.
Virchows Arch 2007 Oct 3;451(4):771-9. Epub 2007 Aug 3.
Division of Molecular Genetics and Biofunction, Department of Biomedical Science, Graduate School of Medicine, Tottori University, 86, Nishi-cho, Yonago 683-8503, Japan.

Ku70/80 modulates ATM and ATR signaling pathways in response to DNA double strand breaks.
J Biol Chem 2007 Apr 1;282(14):10138-45. Epub 2007 Feb 1.
Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University, 86 Nishimachi, Yonago, Tottori 683-8503, Japan.

Antigen-mediated growth control of hybridoma cells via a human artificial chromosome.
Biochim Biophys Acta 2007 Feb 6;1770(2):206-12. Epub 2006 Nov 6.
Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.

Delayed onset of beating and decreased expression of T-type Ca2+ channel in mouse ES cell-derived cardiocytes carrying human chromosome 21.
Biochem Biophys Res Commun 2006 Dec 12;351(1):126-32. Epub 2006 Oct 12.
Division of Regenerative Medicine and Therapeutics, Department of Genetic Medicine and Regenerative Therapeutics, Tottori University Graduate School of Medical Science, 86 Nishi-cho, Yonago 683-8503, Japan.

Expression profile of LIT1/KCNQ1OT1 and epigenetic status at the KvDMR1 in colorectal cancers.
Cancer Sci 2006 Nov 5;97(11):1147-54. Epub 2006 Sep 5.
Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University, 86 Nishimachi, Yonago, Tottori 683-8503, Japan.

Telomere shortening with aging in the human pancreas.
Exp Gerontol 2006 Sep 24;41(9):882-6. Epub 2006 Jul 24.
Human Tissue Research Group, Tokyo Metropolitan Institute of Gerontology, 35-2 Sakaecho, Itabashi-ku, Tokyo 173-0015, Japan.

Production of knockout mice by random or targeted mutagenesis in spermatogonial stem cells.
Proc Natl Acad Sci U S A 2006 May 5;103(21):8018-23. Epub 2006 May 5.
Department of Molecular Genetics, Horizontal Medical Research Organization, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan. tshinoha2virus.kyoto-u.ac.jp


Heat-regulated production and secretion of insulin from a human artificial chromosome vector.
Biochem Biophys Res Commun 2006 Feb 27;340(4):1053-61. Epub 2005 Dec 27.
Department of Human Genome Science, Graduate School of Medical Science, Tottori University, 86 Nishi-cho, Yonago, Tottori 683-8503, Japan.


Evidence for a role of Collapsin response mediator protein-2 in signaling pathways that regulate the proliferation of non-neuronal cells.
Biochem Biophys Res Commun 2006 Feb 5;340(4):1244-50. Epub 2006 Jan 5.
Department of Genetic Medicine and Regenerative Therapeutics, Graduate School of Medical Science, Tottori University, 86 Nishi-cho, Yonago, Tottori 683-8503, Japan.


A novel system for simultaneous or sequential integration of multiple gene-loading vectors into a defined site of a human artificial chromosome.
PLoS One 2014 10;9(10):e110404. Epub 2014 Oct 10.
Stem Cell Project, Tokyo Metropolitan Institute of Medical Science, Kamikitazawa, Setagaya-ku, Tokyo, Japan; Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Yushima, Bunkyo-ku, Tokyo, Japan.


A novel expression system for genomic DNA loci using a human artificial chromosome vector with transformation-associated recombination cloning.
J Hum Genet 2005 18;50(11):592-9. Epub 2005 Oct 18.
Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University, 86 Nishi-cho, Yonago, Tottori 683-8503, Japan.

Deleted in Esophageal Cancer 1(DEC1) is down-regulated and contributes to migration in head and neck squamous cell carcinoma cell lines.
ORL J Otorhinolaryngol Relat Spec 2011 26;73(1):17-23. Epub 2010 Oct 26.
Division of Otolaryngology, Head and Neck Surgery, Department of Medicine of Sensory and Motor Organs, School of Medicine, Tottori University Faculty of Medicine, Tottori, Japan.

Exogenous gene expression and growth regulation of hematopoietic cells via a novel human artificial chromosome.
J Hum Genet 2006 7;51(2):147-50. Epub 2005 Dec 7.
Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University, 86 Nishi-cho, Yonago, Tottori 683-8503, Japan.

A novel human artificial chromosome vector provides effective cell lineage-specific transgene expression in human mesenchymal stem cells.
Stem Cells 2005 Nov-Dec;23(10):1608-16. Epub 2005 Sep 1.
Department of Molecular and Cell Genetics, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University, Yonago, Tottori 683-8503, Japan.


Integration-free iPS cells engineered using human artificial chromosome vectors.
PLoS One 2011 5;6(10):e25961. Epub 2011 Oct 5.
Division of Molecular and Cell Genetics, Department of Molecular and Cellular Biology, School of Life Sciences, Faculty of Medicine, Tottori University, Yonago, Japan.

OF