Publications by authors named "Minxuan Liu"

9 Publications

  • Page 1 of 1

Genetic Divergence and Population Structure in Weedy and Cultivated Broomcorn Millets ( L.) Revealed by Specific-Locus Amplified Fragment Sequencing (SLAF-Seq).

Front Plant Sci 2021 24;12:688444. Epub 2021 Jun 24.

School of Life Sciences, Jilin University, Changchun, China.

Broomcorn millet ( L.) is one of the earliest domesticated crops in the world. Weedy broomcorn millet [ (Kitag.) Chang or subsp. (Kitag.) Tzvel] is thought to be the descendant of the wild ancestor or the feral type of this cereal. The genealogical relationships and genetic divergence among these taxa have not been clarified. In this study, the genetic diversity and population structure of weedy and cultivated broomcorn millets were investigated by using the high-throughput sequencing technology, i.e., the specific-locus amplified fragment sequencing (SLAF-seq). Our analyses consistently revealed both the wild and the feral genotypes in the weedy broomcorn millets. The single nucleotide polymorphisms (SNPs) at the genomic level provided useful evidence to distinguish the wild and the endoferal/exoferal types of weedy broomcorn millets. The genetic divergence revealed between the cultivated broomcorn millet from eastern Eurasia and those from central-western Eurasia was probably derived from either the genetic introgression from weedy broomcorn millets along the spread routes or the founder effect, while the limited gene flow of broomcorn millets from eastern and central-western Eurasia was probably due to the different uses of broomcorn millets and eating habits of the local people.
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http://dx.doi.org/10.3389/fpls.2021.688444DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8264369PMC
June 2021

A broad-spectrum sensing strategy for the tetracycline family of antibiotics based on an ovalbumin-stabilized gold nanocluster and its application in a pump-free microfluidic sensing platform.

Biosens Bioelectron 2021 Jan 11;171:112701. Epub 2020 Oct 11.

College of Food Science and Technology, Hebei Agricultural University, Baoding, 071001, China; Medical College, Nankai University, Tianjin, 300500, China. Electronic address:

With increasing concerns related to the abuse of antibiotics in livestock production worldwide, simple and rapid screening methods for monitoring antibiotics in animal-derived foods are highly desirable. In this study, we propose a facile synthesis strategy for gold nanoclusters (AuNCs) exhibiting remarkable optical properties by employing ovalbumin (OVA) as the template. The OVA-stabilized AuNCs ([email protected]) manifest intriguing multicolour fluorescence and a gradually declining fluorescence intensity at 650 nm with an increasing concentration of tetracycline family antibiotics (TCs) including tetracycline, chlorotetracycline, oxytetracycline, and doxycycline, which are a widely used class of antibiotics for treating infections in food-producing animals. This performance makes [email protected] particularly attractive as a broad-spectrum detector for TCs sensing, and we demonstrate that this simple sensing procedure can be realized in real time by directly mixing the target sample and [email protected] components. Based on this sensing strategy, a microfluidic lab-on-a-chip platform was constructed for the ultrarapid detection of TCs within 30 s. The detection limit was determined to be 0.09 μg/mL in chicken muscle extract, with the recovery ranging from 86.20% to 93.57% in spiked samples. This work provides not only a broad-spectrum sensing strategy for TCs but also a pump-free microfluidic chip with the advantages of being portable, ultrarapid, and low cost, offering a viable alternative for on-the-spot ultrarapid screening of TCs.
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http://dx.doi.org/10.1016/j.bios.2020.112701DOI Listing
January 2021

Analysis of β-d-glucan biosynthetic genes in oat reveals glucan synthesis regulation by light.

Ann Bot 2021 02;127(3):371-380

Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing, China.

Background And Aims: Oat (Avena sativa) has human health benefits when consumed as a whole-grain food, attributed to the high content of (1,3;1,4)-β-d-glucan (mixed-linkage glucan [MLG]), but little is known about the synthase genes and synthesis mechanism of MLG polysaccharides in this species.

Methods: The concentration of oat MLGs under different light intensities was measured by a standard enzymatic approach and further verified by immunoelectron microscopy. The effect of light intensity on MLG synthase genes was examined by RT-qPCR and western blot analyses. The pattern of expression directed by the promoter of the oat MLG synthase gene was also investigated by histochemical β-glucuronidase (GUS) analysis.

Key Results: The oat orthologues of genes implicated in the synthesis of MLG in other cereals, including cellulose synthase-like (Csl) F, H and J gene families, were defined. Transcript profiling of these genes across oat tissues indicated that AsCslF6 transcripts dominated. Under high light intensities, the expression of AsCslF6, a major isoform of the MLG synthase genes, increased to >30 % of the dark growth control. The amount of MLG in oat rose from 0.07 to 1.06 % with increased light intensity. Histochemical tests showed that the AsCslF6 gene promoter preferentially directs GUS expression under high light intensity conditions.

Conclusions: Oat MLG synthesis is regulated by light. High light intensity upregulates the expression of the MLG synthase AsCslF6 gene, leading to an increase in the amount of MLG in oat leaves.
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http://dx.doi.org/10.1093/aob/mcaa185DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7872105PMC
February 2021

Novel Therapeutic Targets for Hypoxia-Related Cardiovascular Diseases: The Role of HIF-1.

Front Physiol 2020 15;11:774. Epub 2020 Jul 15.

State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang, China.

Insufficient oxygen availability (hypoxia) is a precursor to numerous cardiovascular diseases, including atherosclerosis, pulmonary hypertension, and heart failure. The main site of hypoxic injury in the human body is the mitochondria, where oxygen acts as the final electron acceptor in the process of oxidative phosphorylation. Hypoxia-inducible factor (HIF) is activated in hypoxic conditions and acts as an important modulator of diverse target genes in the human body. The downstream genes of HIF include vital modulators of cardiovascular-related signaling pathways. Therefore, it is hypothesized that HIF represents a potential therapeutic target for the treatment and prevention of cardiovascular diseases. In this short review, we introduce the pathophysiology of hypoxic injury in cardiovascular disease, and we conclude from convincing evidence that HIF can modulate relevant cardioprotective signaling pathways.
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http://dx.doi.org/10.3389/fphys.2020.00774DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7375027PMC
July 2020

Development of a Broad-Specific Competitive ELISA for First-Generation Cephalosporin Antibiotics in Animal-Derived Foods Samples.

Bull Environ Contam Toxicol 2021 Aug 29;107(2):215-220. Epub 2020 May 29.

College of Food Science and Technology, Hebei Agricultural University, Baoding, 071001, China.

The abuse of antibiotics, such as the cephalosporins in livestock and aquaculture productions, usually causes the widespread antibiotic resistance due to their growth-promoting effects. In this study, cephalexin was chosen as the hapten molecule to prepare a broad-spectrum rabbit polyclonal antibody for cephalosporin antibiotics. The obtained antibody exhibited broad cross-reactivity ranging from 0.05% to 100% with 10 cephalosporins. Based on this antibody, we developed a broad-specific indirect competitive ELISA (ic-ELISA) for cefalexin, cefradine, cefadroxil and cefazolin with the half maximal inhibitory concentration (IC) ranging from 0.72 to 2.99 ng/mL in working buffer. For animal-derived food samples with spiked cephalosporins, the ic-ELISA exhibited an excellent recovery ranging from 72.3% to 95.6%. To verify the accuracy of this proposed ic-ELISA, its detection performance was evaluated utilizing the high-performance liquid chromatography with satisfactory results. This study confirmed that: firstly, the prepared antibody can be used as a class-specific recognition element to develop immunoassays for cephalosporin antibiotics; and secondly, the developed ic-ELISA provided a new tool for broad-spectrum detection of first-generation cephalosporins in animal-derived foods.
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http://dx.doi.org/10.1007/s00128-020-02885-yDOI Listing
August 2021

Chromosome conformation capture resolved near complete genome assembly of broomcorn millet.

Nat Commun 2019 01 25;10(1):464. Epub 2019 Jan 25.

State Key Laboratory of Agrobiotechnology and National Maize Improvement Center, Department of Plant Genetics and Breeding, China Agricultural University, Beijing, 100193, P. R. China.

Broomcorn millet (Panicum miliaceum L.) has strong tolerance to abiotic stresses, and is probably one of the oldest crops, with its earliest cultivation that dated back to ca. ~10,000 years. We report here its genome assembly through a combination of PacBio sequencing, BioNano, and Hi-C (in vivo) mapping. The 18 super scaffolds cover ~95.6% of the estimated genome (~887.8 Mb). There are 63,671 protein-coding genes annotated in this tetraploid genome. About ~86.2% of the syntenic genes in foxtail millet have two homologous copies in broomcorn millet, indicating rare gene loss after tetraploidization in broomcorn millet. Phylogenetic analysis reveals that broomcorn millet and foxtail millet diverged around ~13.1 Million years ago (Mya), while the lineage specific tetraploidization of broomcorn millet may be happened within ~5.91 million years. The genome is not only beneficial for the genome assisted breeding of broomcorn millet, but also an important resource for other Panicum species.
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http://dx.doi.org/10.1038/s41467-018-07876-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6347627PMC
January 2019

Phytolith analysis for differentiating between broomcorn millet (Panicum miliaceum) and its weed/feral type (Panicum ruderale).

Sci Rep 2018 08 29;8(1):13022. Epub 2018 Aug 29.

Key Laboratory of Cenozoic Geology and Environment, Institute of Geology and Geophysics, Chinese Academy of Sciences, Beijing, 100029, China.

Domestication of broomcorn millet (Panicum miliaceum) is one of the most significant events in prehistoric East Asia, providing sufficient food supply for the explosive growth of Neolithic populations and the transition into complex societies. However, to date, the process of broomcorn millet domestication is still largely unknown, partly due to the lack of clear diagnostic tools for distinguishing between millet and its related wild grasses in archaeological samples. Here, we examined the percentage of silicified epidermal long-cell undulated patterns in the glume and palea from inflorescence bracts in 21 modern varieties of broomcorn millet and 12 weed/feral-type Panicum ruderale collected across northern China. Our results show that the percentage of ηIII patterns in domesticated broomcorn millet (23.0% ± 5.9%; n = 63) is about 10% higher than in P. ruderale (10.8% ± 5.8%; n = 36), with quartiles of 17.2-28.3% and 5.1-15.5%, respectively. Owing to the increase in ηIII pattern percentage correlates significantly with a decrease in the grain length/width ratio, in the absence of exact wild ancestors of broomcorn millet, the characterization of phytolith differences between P. ruderale and P. miliaceum thus becomes an alternative approach to provide insight into origin of broomcorn millet.
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http://dx.doi.org/10.1038/s41598-018-31467-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6115419PMC
August 2018

Transcriptome analysis of filling stage seeds among three buckwheat species with emphasis on rutin accumulation.

PLoS One 2017 20;12(12):e0189672. Epub 2017 Dec 20.

The Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.

Buckwheat is an important minor crop with pharmaceutical functions due to rutin enrichment in the seed. Seeds of common buckwheat cultivars (Fagopyrum esculentum, Fes) usually have much lower rutin content than tartary buckwheat (F. tartaricum, Ft). We previously found a wild species of common buckwheat (F. esculentum ssp. ancestrale, Fea), with seeds that are high in rutin, similar to Ft. In the present study, we investigated the mechanism by which rutin production varies among different buckwheat cultivars, Fea, a Ft variety (Xide) and a Fes variety (No.2 Pingqiao) using RNA sequencing of filling stage seeds. Sequencing data generated approximately 43.78-Gb of clean bases, all these data were pooled together and assembled 180,568 transcripts, and 109,952 unigenes. We established seed gene expression profiles of each buckwheat sample and assessed genes involved in flavonoid biosynthesis, storage proteins production, CYP450 family, starch and sucrose metabolism, and transcription factors. Differentially expressed genes between Fea and Fes were further analyzed due to their close relationship than with Ft. Expression levels of flavonoid biosynthesis gene FLS1 (Flavonol synthase 1) were similar in Fea and Ft, and much higher than in Fes, which was validated by qRT-PCR. This suggests that FLS1 transcript levels may be associated with rutin accumulation in filling stage seeds of buckwheat species. Further, we explored transcription factors by iTAK, and multiple gene families were identified as being involved in the coordinate regulation of metabolism and development. Our extensive transcriptomic data sets provide a complete description of metabolically related genes that are differentially expressed in filling stage buckwheat seeds and suggests that FLS1 is a key controller of rutin synthesis in buckwheat species. FLS1 can effectively convert dihydroflavonoids into flavonol products. These findings provide a basis for further studies of flavonoid biosynthesis in buckwheat breeding to help accelerate flavonoid metabolic engineering that would increase rutin content in cultivars of common buckwheat.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0189672PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5738128PMC
January 2018

Genetic Diversity and Population Structure of Broomcorn Millet (Panicum miliaceum L.) Cultivars and Landraces in China Based on Microsatellite Markers.

Int J Mol Sci 2016 Mar 14;17(3):370. Epub 2016 Mar 14.

Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.

Broomcorn millet (Panicum miliaceum L.), one of the first domesticated crops, has been grown in Northern China for at least 10,000 years. The species is presently a minor crop, and evaluation of its genetic diversity has been very limited. In this study, we analyzed the genetic diversity of 88 accessions of broomcorn millet collected from various provinces of China. Amplification with 67 simple sequence repeat (SSR) primers revealed moderate levels of diversity in the investigated accessions. A total of 179 alleles were detected, with an average of 2.7 alleles per locus. Polymorphism information content and expected heterozygosity ranged from 0.043 to 0.729 (mean = 0.376) and 0.045 to 0.771 (mean = 0.445), respectively. Cluster analysis based on the unweighted pair group method of mathematical averages separated the 88 accessions into four groups at a genetic similarity level of 0.633. A genetic structure assay indicated a close correlation between geographical regions and genetic diversity. The uncovered information will be valuable for defining gene pools and developing breeding programs for broomcorn millet. Furthermore, the millet-specific SSR markers developed in this study should serve as useful tools for assessment of genetic diversity and elucidation of population structure in broomcorn millet.
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http://dx.doi.org/10.3390/ijms17030370DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4813230PMC
March 2016
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