Publications by authors named "Mintao Sun"

8 Publications

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Correction to: The CsGPA1-CsAQPs module is essential for salt tolerance of cucumber seedlings.

Plant Cell Rep 2021 Oct;40(10):2015-2016

The Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Haidian District, Zhongguancun South St, Beijing, 100081, China.

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http://dx.doi.org/10.1007/s00299-021-02782-6DOI Listing
October 2021

Sugars promote graft union development in the heterograft of cucumber onto pumpkin.

Hortic Res 2021 Jul 1;8(1):146. Epub 2021 Jul 1.

Institute of Vegetables and Flowers, Chinese Academy of Agriculture Sciences, Beijing, 100081, China.

The use of heterografts is widely applied for the production of several important commercial crops, but the molecular mechanism of graft union formation remains poorly understood. Here, cucumber grafted onto pumpkin was used to study graft union development, and genome-wide tempo-spatial gene expression at the graft interface was comprehensively investigated. Histological analysis suggested that resumption of the rootstock growth occurred after both phloem and xylem reconnection, and the scion showed evident callus production compared with the rootstock 3 days after grafting. Consistently, transcriptome data revealed specific responses between the scion and rootstock in the expression of genes related to cambium development, the cell cycle, and sugar metabolism during both vascular reconnection and healing, indicating distinct mechanisms. Additionally, lower levels of sugars and significantly changed sugar enzyme activities at the graft junction were observed during vascular reconnection. Next, we found that the healing process of grafted etiolated seedlings was significantly delayed, and graft success, xylem reconnection, and the growth of grafted plants were enhanced by exogenous glucose. This demonstrates that graft union formation requires the correct sugar content. Furthermore, we also found that graft union formation was delayed with a lower energy charge by the target of rapamycin (TOR) inhibitor AZD-8055, and xylem reconnection and the growth of grafted plants were enhanced under AZD-8055 with exogenous glucose treatment. Taken together, our results reveal that sugars play a positive role in graft union formation by promoting the growth of cucumber/pumpkin and provide useful information for understanding graft union healing and the application of heterografting in the future.
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http://dx.doi.org/10.1038/s41438-021-00580-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8245404PMC
July 2021

Adaptation of cucumber seedlings to low temperature stress by reducing nitrate to ammonium during it's transportation.

BMC Plant Biol 2021 Apr 19;21(1):189. Epub 2021 Apr 19.

The Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.

Background: Low temperature severely depresses the uptake, translocation from the root to the shoot, and metabolism of nitrate and ammonium in thermophilic plants such as cucumber (Cucumis sativus). Plant growth is inhibited accordingly. However, the availability of information on the effects of low temperature on nitrogen transport remains limited.

Results: Using non-invasive micro-test technology, the net nitrate (NO) and ammonium (NH) fluxes in the root hair zone and vascular bundles of the primary root, stem, petiole, midrib, lateral vein, and shoot tip of cucumber seedlings under normal temperature (NT; 26 °C) and low temperature (LT; 8 °C) treatment were analyzed. Under LT treatment, the net NO flux rate in the root hair zone and vascular bundles of cucumber seedlings decreased, whereas the net NH flux rate in vascular bundles of the midrib, lateral vein, and shoot tip increased. Accordingly, the relative expression of CsNRT1.4a in the petiole and midrib was down-regulated, whereas the expression of CsAMT1.2a-1.2c in the midrib was up-regulated. The results of N isotope tracing showed that NO-N and NH-N uptake of the seedlings under LT treatment decreased significantly compared with that under NT treatment, and the concentration and proportion of both NO-N and NH-N distributed in the shoot decreased. Under LT treatment, the actual nitrate reductase activity (NRA) in the root did not change significantly, whereas NRA in the stem and petiole increased by 113.2 and 96.2%, respectively.

Conclusions: The higher net NH flux rate in leaves and young tissues may reflect the higher NRA in the stem and petiole, which may result in a higher proportion of NO being reduced to NH during the upward transportation of NO. The results contribute to an improved understanding of the mechanism of changes in nitrate transportation in plants in response to low-temperature stress.
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http://dx.doi.org/10.1186/s12870-021-02918-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8056598PMC
April 2021

The CsGPA1-CsAQPs module is essential for salt tolerance of cucumber seedlings.

Plant Cell Rep 2020 Oct 9;39(10):1301-1316. Epub 2020 Jul 9.

The Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Haidian District, Zhongguancun South St, Beijing, 100081, China.

Key Message: CsGPA1 interacts with CsTIP1.1 (a member of CsAQPs) and suppression of CsGPA1 results the reverse expression of CsAQPs in leaves and roots, resulting in declining water content of cucumber seedlings under salt stress. Salt stress seriously affects cucumber growth and development. Whether the G-protein alpha subunit functions in cucumber during salt stress and its regulation mechanism remains unknown. We interrogated CsGPA1-RNAi lines to identify the role of CsGPA1 during salt stress. Phenotypically, compared with wild type, leaves were severely withered, and root cells showed signs of senescence under salt stress for RNAi lines. Compared with WT, SOD and CAT activity, soluble protein and proline contents all decreased in RNAi lines, while malondialdehyde and relative electrical conductivity increased. Through screening the yeast two-hybrid library and combined with yeast two-hybrid and GST pull-down, the interaction of CsGPA1 with CsTIP1.1 was found the first time in a plant. Then, the expression of aquaporin (AQP) family genes was detected. The expression of CsAQP genes in leaves and roots was primarily up-regulated in WT under salt stress. However, interference by CsGPA1 resulted in enhanced expression of CsAQPs except for CsTIP3.2 in leaves, but reduced expression of some CsAQPs in roots under salt stress. Furthermore, principal component analysis of CsAQP expression profiles and linear regression analysis between CsGPA1 and CsAQPs revealed that CsGPA1 reversely regulated the expression of CsAQPs in leaves and roots under salt stress. Moreover, the water content in leaves and roots of RNAi seedlings significantly decreased compared with WT under salt stress. Overall, CsGPA1 interacts with CsTIP1.1 and suppression of CsGPA1 results in opposite patterns of expression of CsAQPs in leaves and roots, resulting in declining water content of cucumber under salt stress.
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http://dx.doi.org/10.1007/s00299-020-02565-5DOI Listing
October 2020

LncRNA regulates tomato fruit cracking by coordinating gene expression via a hormone-redox-cell wall network.

BMC Plant Biol 2020 Apr 15;20(1):162. Epub 2020 Apr 15.

College of Horticulture, Nanjing Agricultural University, Weigang NO 1, Nanjing, 210095, Xuanwu District, China.

Background: Fruit cracking occurs easily under unsuitable environmental conditions and is one of the main types of damage that occurs in fruit production. It is widely accepted that plants have developed defence mechanisms and regulatory networks that respond to abiotic stress, which involves perceiving, integrating and responding to stress signals by modulating the expression of related genes. Fruit cracking is also a physiological disease caused by abiotic stress. It has been reported that a single or several genes may regulate fruit cracking. However, almost none of these reports have involved cracking regulatory networks.

Results: Here, RNA expression in 0 h, 8 h and 30 h saturated irrigation-treated fruits from two contrasting tomato genotypes, 'LA1698' (cracking-resistant, CR) and 'LA2683' (cracking-susceptible, CS), was analysed by mRNA and lncRNA sequencing. The GO pathways of the differentially expressed mRNAs were mainly enriched in the 'hormone metabolic process', 'cell wall organization', 'oxidoreductase activity' and 'catalytic activity' categories. According to the gene expression analysis, significantly differentially expressed genes included Solyc02g080530.3 (Peroxide, POD), Solyc01g008710.3 (Mannan endo-1,4-beta-mannosidase, MAN), Solyc08g077910.3 (Expanded, EXP), Solyc09g075330.3 (Pectinesterase, PE), Solyc07g055990.3 (Xyloglucan endotransglucosylase-hydrolase 7, XTH7), Solyc12g011030.2 (Xyloglucan endotransglucosylase-hydrolase 9, XTH9), Solyc10g080210.2 (Polygalacturonase-2, PG2), Solyc08g081010.2 (Gamma-glutamylcysteine synthetase, gamma-GCS), Solyc09g008720.2 (Ethylene receptor, ER), Solyc11g042560.2 (Ethylene-responsive transcription factor 4, ERF4) etc. In addition, the lncRNAs (XLOC_16662 and XLOC_033910, etc) regulated the expression of their neighbouring genes, and genes related to tomato cracking were selected to construct a lncRNA-mRNA network influencing tomato cracking.

Conclusions: This study provides insight into the responsive network for water-induced cracking in tomato fruit. Specifically, lncRNAs regulate the hormone-redox-cell wall network, including plant hormone (auxin, ethylene) and ROS (HO) signal transduction and many cell wall-related mRNAs (EXP, PG, XTH), as well as some lncRNAs (XLOC_16662 and XLOC_033910, etc.).
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http://dx.doi.org/10.1186/s12870-020-02373-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7161180PMC
April 2020

Identification of heat-tolerance QTLs and high-temperature stress-responsive genes through conventional QTL mapping, QTL-seq and RNA-seq in tomato.

BMC Plant Biol 2019 Sep 11;19(1):398. Epub 2019 Sep 11.

College of Horticulture, Nanjing Agricultural University, Weigang NO 1, Nanjing, 210095, China.

Background: High temperature is one of the major abiotic stresses in tomato and greatly reduces fruit yield and quality. Identifying high-temperature stress-responsive (HSR) genes and breeding heat-tolerant varieties is an effective way to address this issue. However, there are few reports on the fine mapping of heat-tolerance quantitative trait locus (QTL) and the identification of HSR genes in tomato. Here, we applied three heat tolerance-related physiological indexes, namely, relative electrical conductivity (REC), chlorophyll content (CC) and maximum photochemical quantum efficiency (F/F) of PSII (photosystem II), as well as the phenotypic index, the heat injury index (HII), and conventional QTL analysis combined with QTL-seq technology to comprehensively detect heat-tolerance QTLs in tomato seedlings. In addition, we integrated the QTL mapping results with RNA-seq to identify key HSR genes within the major QTLs.

Results: A total of five major QTLs were detected: qHII-1-1, qHII-1-2, qHII-1-3, qHII-2-1 and qCC-1-5 (qREC-1-3). qHII-1-1, qHII-1-2 and qHII-1-3 were located, respectively, in the intervals of 1.43, 1.17 and 1.19 Mb on chromosome 1, while the interval of qHII-2-1 was located in the intervals of 1.87 Mb on chromosome 2. The locations observed with conventional QTL mapping and QTL-seq were consistent. qCC-1-5 and qREC-1-3 for CC and REC, respectively, were located at the same position by conventional QTL mapping. Although qCC-1-5 was not detected in QTL-seq analysis, its phenotypic variation (16.48%) and positive additive effect (0.22) were the highest among all heat tolerance QTLs. To investigate the genes involved in heat tolerance within the major QTLs in tomato, RNA-seq analysis was performed, and four candidate genes (SlCathB2, SlGST, SlUBC5, and SlARG1) associated with heat tolerance were finally detected within the major QTLs by DEG analysis, qRT-PCR screening and biological function analysis.

Conclusions: In conclusion, this study demonstrated that the combination of conventional QTL mapping, QTL-seq analysis and RNA-seq can rapidly identify candidate genes within major QTLs for a complex trait of interest to replace the fine-mapping process, thus greatly shortening the breeding process and improving breeding efficiency. The results have important applications for the fine mapping and identification of HSR genes and breeding for improved thermotolerance.
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http://dx.doi.org/10.1186/s12870-019-2008-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6739936PMC
September 2019

Respiratory burst oxidase homologue-dependent H O and chloroplast H O are essential for the maintenance of acquired thermotolerance during recovery after acclimation.

Plant Cell Environ 2018 10 3;41(10):2373-2389. Epub 2018 Jul 3.

College of Horticulture, Nanjing Agricultural University, Nanjing, China.

Thermotolerance is improved by heat stress (HS) acclimation, and the thermotolerance level is "remembered" by plants. However, the underlying signalling mechanisms remain largely unknown. Here, we showed NADPH oxidase-mediated H O (NADPH-H O ), and chloroplast-H O promoted the sustained expression of HS-responsive genes and programmed cell death (PCD) genes, respectively, during recovery after HS acclimation. When spraying the NADPH oxidase inhibitor, diphenylene iodonium, after HS acclimation, the NADPH-H O level significantly decreased, resulting in a decrease in the expression of HS-responsive genes and the loss of maintenance of acquired thermotolerance (MAT). In contrast, compared with HS acclimation, NADPH-H O declined but chloroplast-H O further enhanced during recovery after HS over-acclimation, resulting in the reduced expression of HS-responsive genes and substantial production of PCD. Notably, the further inhibition of NADPH-H O after HS over-acclimation also inhibited chloroplast-H O , alleviating the severe PCD and surpassing the MAT of HS over-acclimation treatment. Due to the change in subcellular H O after HS acclimation, the tomato seedlings maintained a constant H O level during recovery, resulting in stable and lower total H O levels during a tester HS challenge conducted after recovery. We conclude that tomato seedlings increase their MAT by enhancing NADPH-H O content and controlling chloroplast-H O production during recovery, which enhances the expression of HS-responsive genes and balances PCD levels, respectively.
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http://dx.doi.org/10.1111/pce.13351DOI Listing
October 2018

Identification of miRNAs and their targets in wild tomato at moderately and acutely elevated temperatures by high-throughput sequencing and degradome analysis.

Sci Rep 2016 Sep 22;6:33777. Epub 2016 Sep 22.

College of Horticulture, Nanjing Agricultural University, Weigang No. 1, Nanjing, 210095, China.

MicroRNAs (miRNAs) are 19-24 nucleotide (nt) noncoding RNAs that play important roles in abiotic stress responses in plants. High temperatures have been the subject of considerable attention due to their negative effects on plant growth and development. Heat-responsive miRNAs have been identified in some plants. However, there have been no reports on the global identification of miRNAs and their targets in tomato at high temperatures, especially at different elevated temperatures. Here, three small-RNA libraries and three degradome libraries were constructed from the leaves of the heat-tolerant tomato at normal, moderately and acutely elevated temperatures (26/18 °C, 33/33 °C and 40/40 °C, respectively). Following high-throughput sequencing, 662 conserved and 97 novel miRNAs were identified in total with 469 conserved and 91 novel miRNAs shared in the three small-RNA libraries. Of these miRNAs, 96 and 150 miRNAs were responsive to the moderately and acutely elevated temperature, respectively. Following degradome sequencing, 349 sequences were identified as targets of 138 conserved miRNAs, and 13 sequences were identified as targets of eight novel miRNAs. The expression levels of seven miRNAs and six target genes obtained by quantitative real-time PCR (qRT-PCR) were largely consistent with the sequencing results. This study enriches the number of heat-responsive miRNAs and lays a foundation for the elucidation of the miRNA-mediated regulatory mechanism in tomatoes at elevated temperatures.
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http://dx.doi.org/10.1038/srep33777DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5031959PMC
September 2016
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