Publications by authors named "Mingyang Li"

306 Publications

Numerical Simulation and Experimental Study the Effects of Process Parameters on Filament Morphology and Mechanical Properties of FDM 3D Printed PLA/GNPs Nanocomposite.

Polymers (Basel) 2022 Jul 29;14(15). Epub 2022 Jul 29.

Industry Engineering Department, School of Mechanical Engineering, Northwestern Polytechnical University, Xi'an 710072, China.

The selection of optimal process parameters has a decisive effect on the quality of 3D printing. In this work, the numerical and experimental methods were employed to investigate the FDM printing deposition process of PLA/GNPs nanocomposite. The effect of process parameters on cross-sectional morphology and dimension of the deposited filament, as well as the mechanical property of the FDM printed specimens were studied. The extrusion and the deposition process of the molten PLA/GNPs nanocomposite was simulated as a fluid flow by the paradigm of CFD, the effects of printing temperature and shear rate on thermal-physical properties, such as viscosity and surface tension, were considered in models. Under the assumptions of non-Newtonian fluid and creep laminar flow, the deposition flow was controlled by two key parameters: the nozzle temperature and the nozzle velocity. The numerical model was verified by experiments from four aspects of thickness, width, area, and compactness of the deposited PLA/GNPs nanocomposite filament cross-section. Both the numerical simulation and experiment results show that with the increase of nozzle temperature and nozzle velocity, the thickness, area, and compactness of the deposited filament decreases. While the width of deposited filament increased with the increase of nozzle temperature and decrease of nozzle velocity. The decrease in thickness and the increase in width caused by the change of process parameters reached 10.5% and 24.7%, respectively. The tensile strength of the printed PLA/GNPs specimen was about 61.8 MPa under the higher nozzle temperatures and velocity condition, an improvement of 18.6% compared to specimen with the tensile strength of 52.1 MPa under the lower nozzle temperatures and velocity condition. In addition, the experimental results indicated that under the low nozzle velocity and nozzle temperature condition, dimensional standard deviation of the printed specimens decreased by 52.2%, 62.7%, and 68.3% in X, Y, and Z direction, respectively.
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http://dx.doi.org/10.3390/polym14153081DOI Listing
July 2022

A novel umami electrochemical biosensor based on [email protected]/TiC MXene immobilized T1R1-VFT.

Food Chem 2022 Jul 30;397:133838. Epub 2022 Jul 30.

Department of Food Science & Technology, School of Agriculture & Biology, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address:

The bioelectronic tongues based on taste receptors have been emerging with human-like taste perception. However, the practical applications of the receptor-based biosensors were restricted by their narrow and low dynamic ranges. Here, a novel immobilization strategy based on [email protected]/TiC MXene was developed to immobilize the umami ligand binding domain (T1R1-VFT), to fabricate an umami biosensor for umami substances detection. Through the synergic effect of [email protected] and TiC MXene, the capacity to load T1R1-VFT was effectively increased, and the response signal was also amplified by approximately 3 times. The proposed biosensor showed an ultrawide dynamic range of 10-10 M, and a high upper limit of detection, which was closer to the human taste threshold and suitable for detecting foods rich in umami substances. Additionally, the biosensor was successfully applied to detect real samples and analyze the synergistic effects of binary umami substances.
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http://dx.doi.org/10.1016/j.foodchem.2022.133838DOI Listing
July 2022

Cancer stem cells in esophageal squamous cell carcinoma.

Pathol Res Pract 2022 Jul 30;237:154043. Epub 2022 Jul 30.

State Key Laboratory of Cancer Biology, Department of Pathology, Xijing Hospital and School of Basic Medicine, Fourth Military Medical University, Xi'an, China. Electronic address:

Esophageal squamous cell carcinoma (ESCC) is one of the most intractable challenges among human diseases with poor prognosis and shortness of effective therapeutic options. Cancer stem cells (CSCs), a tumor sub-population, are considered the cause of tumor growth, differentiation, metastasis and therapeutic resistance, etc. In this review, we discuss the known methods for isolation and verification of ESCC CSCs, the biomarkers of ESCC CSCs and their significance in diagnosis and prognosis. Then we review the ESCC CSC signaling pathway and therapeutic resistance. In pace with the detailed studies of ESCC CSCs increasing, treatment strategies of ESCC based on CSCs are becoming more promising.
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http://dx.doi.org/10.1016/j.prp.2022.154043DOI Listing
July 2022

Developmental pattern of association fibers and their interaction with associated cortical microstructures in 0-5-month-old infants.

Neuroimage 2022 Jul 28;261:119525. Epub 2022 Jul 28.

Key Laboratory for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, College of Biomedical Engineering & Instrument Science, Zhejiang University, Hangzhou 310027, China. Electronic address:

Association fibers connect the cortical regions and experience rapid development involving myelination and axonal growth during infancy. Yet, the spatiotemporal patterns of microstructural changes along these tracts, as well as the developmental interaction between the white matter (WM) tracts and the cortical gray matter (cGM) connected to them, are mostly unknown during infancy. In this study, we performed a diffusion MRI-based tractography and microstructure study in a cohort of 89 healthy preterm-born infants with gestational age at birth between 28.1∼36.4 weeks and postmenstrual age at scan between 39.9∼59.9 weeks. Results revealed that several C-shaped fibers, such as the arcuate fasciculus, cingulum, and uncinate fasciculus, demonstrated symmetrical along-tract profiles; and the horizontally oriented running fibers, including the inferior fronto-occipital fasciculus and the inferior longitudinal fasciculus, demonstrated an anterior-posterior developmental gradient. This study characterized the along-tract profiles using fixel-based analysis and revealed that the fiber cross-section (FC) of all five association fibers demonstrated a fluctuating increase with age, while the fiber density (FD) monotonically increase with age. NODDI was utilized to analyze the microstructural development of cGM and indicated cGM connected to the anterior end of the association fibers developed faster than that of the posterior end during 0-5 months. Notably, a mediation analysis was used to explore the relation between the development of WM and associated cGM, and demonstrated a partial mediation effect of FD in WM on the development of intracellular volume (ICV) in cGM and a full mediation effect of ICV on the growth of FD in most fibers, suggesting a predominant mediation of cGM on the WM development. Furthermore, for assessing whether those results were biased by prematurity, we compared preterm- and term-born neonates with matched scan age, gender, and multiple births from the developing human connectome project (dHCP) dataset to assess the effect of preterm-birth, and the results indicated a similar developmental pattern of the association fibers and their attached cGM. These findings presented a comprehensive picture of the major association fibers during early infancy and deciphered the developmental interaction between WM and cGM in this period.
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http://dx.doi.org/10.1016/j.neuroimage.2022.119525DOI Listing
July 2022

Stochastic Local Breakdown of Oxide Film on Ni from Identical-Location Imaging: One Single Site at a Time.

Nano Lett 2022 Aug 25;22(15):6313-6319. Epub 2022 Jul 25.

Department of Chemistry, The University of Texas at Austin, Austin, Texas 78712, United States.

The electrochemical breakdown of a metal oxide film can directly affect the performance of functional electrochemical devices. However, revealing the structural insight into the breakdown sites is challenging because of heterogeneity: different breakdown sites are spatially distributed over the surface. Herein, we combine scanning electrochemical cell microscopy with identical-location microscopies to reveal the heterogeneity in the breakdown of NiO film on Ni in a site-by-site manner. Local critical breakdown potential varies by ∼500 mV, corresponding to an excess energy of 0.02-0.12 J/m. Correlative composition imaging using time-of-flight secondary ion mass spectrometry shows Ni crystal grains with thinner NiO films are more resistant to breakdown. This high resistance is explained using classical nucleation theory, where the electrical energy is affected by the film thickness through the local interfacial capacitance. The correlative imaging approach overcomes the issue of heterogeneity, providing conclusive insight into the stability of the electrochemical interfaces.
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http://dx.doi.org/10.1021/acs.nanolett.2c02018DOI Listing
August 2022

Effect of Coated Diammonium Phosphate Combined with Extracts on Soil Available Nutrients, Photosynthesis-Related Enzyme Activities, Endogenous Hormones, and Maize Yield.

ACS Omega 2022 Jul 29;7(27):23566-23575. Epub 2022 Jun 29.

National Engineering Research Center for Efficient Utilization of Soil and Fertilizer Resources, College of Resources and Environment, Shandong Agricultural University, Taian 271018, Shandong, China.

Coated diammonium phosphate (CDAP) is intended to release nutrients steadily in response to the demand of crop growth. A novel biostimulant extracted from has been shown to regulate gene expression in nutrient transport, enhance nitrogen (N) and phosphorus (P) uptake, and improve nutrient use efficiency. The application of CDAP combined with the extracts (ZNC) in maize is an efficient approach for reducing waste of resources, improving nutrient supply, and maintaining production stability. The effects of CDAP combined with ZNC on photosynthesis, enzyme activities, endogenous hormone content, maize yield, and P use efficiency (PUE) were investigated in this study. In a pot experiment, CDAP and diammonium phosphate (DAP) were tested together with P levels (1.80, 1.44 g pot, PO) and two ZNC application rates (0, 4.4 μg pot), which included the control treatment that had no P fertilizer added. Results showed that the key influencing elements of maize growth and yield were the soil available-P content, endogenous hormone content, and plant photosynthesis in this study. The combination of DAP and ZNC increased the soil available-P content and the auxin content in leaves at the key stage and hence increased the yield and PUE of maize, compared with DAP. The net photosynthetic rate of CDAP combined with ZNC was higher by 23.1% than that of CDAP alone, as well as by 32.0% than that of DAP combined with ZNC. Moreover, the combination of CDAP and ZNC increased the yield and PUE by 8.2% and 15.6 percentage points compared with DAP combined with ZNC while increasing the yield and PUE compared with CDAP. In conclusion, combining CDAP with ZNC as an environmentally friendly fertilizer could improve photosynthesis-related enzyme activity and enhance the net photosynthetic rate, resulting in an increase in maize yield and PUE significantly.
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http://dx.doi.org/10.1021/acsomega.2c02102DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9281325PMC
July 2022

Nanoparticle elasticity affects systemic circulation lifetime by modulating adsorption of apolipoprotein A-I in corona formation.

Nat Commun 2022 Jul 16;13(1):4137. Epub 2022 Jul 16.

Hefei National Research Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, 230026, Anhui, China.

Nanoparticle elasticity is crucial in nanoparticles' physiological fate, but how this occurs is largely unknown. Using core-shell nanoparticles with a same PEGylated lipid bilayer shell yet cores differing in elasticity (45 kPa - 760 MPa) as models, we isolate the effects of nanoparticle elasticity from those of other physiochemical parameters and, using mouse models, observe a non-monotonic relationship of systemic circulation lifetime versus nanoparticle elasticity. Incubating our nanoparticles in mouse plasma provides protein coronas varying non-monotonically in composition depending on nanoparticle elasticity. Particularly, apolipoprotein A-I (ApoA1) is the only protein whose relative abundance in corona strongly correlates with our nanoparticles' blood clearance lifetime. Notably, similar results are observed when above nanoparticles' PEGylated lipid bilayer shell is changed to be non-PEGylated. This work unveils the mechanisms by which nanoparticle elasticity affects nanoparticles' physiological fate and suggests nanoparticle elasticity as a readily tunable parameter in future rational exploiting of protein corona.
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http://dx.doi.org/10.1038/s41467-022-31882-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9288426PMC
July 2022

The Expression of MDM2 Gene Promoted Chondrocyte Proliferation in Rats with Osteoarthritis via the Wnt/β-Catenin Pathway.

Cell Mol Biol (Noisy-le-grand) 2022 Feb 27;67(6):236-241. Epub 2022 Feb 27.

Department of Bone Traumatology, Affiliated Hospital of Hunan Academy of Chinese Medicine, Changsha, Hunan410006, China.

This study aimed to investigate the regulatory mechanism of MDM2 gene expression on cartilage cell proliferation in Osteoarthritis (OA) rats. For this purpose, 22 SD rats were randomly divided into normal control (10 cases) and treated (12 cases) groups. Treated group was used for OA modelling with the modified Hulth method. After a week, RT-PCR was used to detect MDM2 in cartilage tissue of rats, Wnt 1, Wnt 3 a, Wnt 10 b and β-catenin genes mRNA expression. Rat chondrocytes were isolated and cultured, and the recombinant eukaryotic expression vector pcDNA3.1 myc-siRNA-MDM2-β-catenin and co-expression plasmid pcDNA3.1 myc-siRNA-MDM2-β-catenin was used to transfect chondrocytes and the proliferation and related gene expression levels of the transfected chondrocytes were detected by MTT method and RT-PCR. The results showed that compared with the control group, MDM2, Wnt 1, Wnt 3 a, Wnt 10b and β-catenin genes in OA rat cartilage constructed by Hulth method were increased (p<0.05). The pcDNA3.1 myc-beta-catenin transfection slowed down the proliferation of OA chondrocytes, different from the non-transfected OA group (p<0.001), and increased Wnt 1, Wnt 3a, Wnt 10b and β-catenin genes expression compared with the Control group (p<0.05), but did not affect the expression of MDM2. The transfection of siRNA-MDM2 was opposite to pcDNA3.1 myc-β-catenin. The co-expression plasmid pcDNA3.1 myc-siRNA-MDM2-beta-catenin transfection did not affect the proliferation of OA chondrocytes. In general, the high expression of MDM2 in OA rats restricts the proliferation of chondrocytes, which may be related to the main pathogenesis of the occurrence and development of OA in vivo, and the regulation of MDM2 on the proliferation of chondrocytes may be achieved through the Wnt/ β-catenin pathway.
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http://dx.doi.org/10.14715/cmb/2021.67.6.31DOI Listing
February 2022

Surgical Treatment of Intraductal Papillary Neoplasm of the Bile Duct: A Report of Two Cases and Review of the Literature.

Front Oncol 2022 23;12:916457. Epub 2022 Jun 23.

Department of Hepatobiliary Surgery, Tianjin Medical University General Hospital, Tianjin, China.

Intraductal papillary neoplasm of the bile duct (IPNB) is a rare bile duct tumor characterized by intraductal papillary or villous neoplasms covered by neoplastic epithelium with fine fibrovascular stalks in the dilated bile ducts (1). Its true etiology remains unknown. Herein, we report two cases of IPNB that underwent surgical resection. The first case was a 66-year-old male who complained of upper abdominal pain for three years. We found obstruction of the common bile duct and dilation of the intrahepatic and extrahepatic bile ducts after MRCP. Laparoscopic hepatic segmentectomy (S2, S3, S4), resection of the common bile duct, cholecystectomy, and hepaticojejunostomy were performed. The second case was a 67-year-old male with asymptomatic dilation of the intrahepatic duct. The patient underwent robot-assisted laparoscopic hepatic segmentectomy (S5, S6, S7, S8), resection of the common bile duct, hepaticojejunostomy and cholecystectomy.
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http://dx.doi.org/10.3389/fonc.2022.916457DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9259795PMC
June 2022

Comprehensive Identification of Short and Medium-Sized Peptides from Pixian Broad Bean Paste Protein Hydrolysates Using UPLC-Q-TOF-MS and UHPLC-Q Exactive HF-X.

J Agric Food Chem 2022 Jul 2;70(27):8288-8299. Epub 2022 Jul 2.

Lab of Brewing Microbiology and Applied Enzymology, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu 214122, P. R. China.

Pixian broad bean paste (PBBP) is an indispensable food widely used in many East Asian countries, yet the knowledge about bioactive peptides released from parent proteins by enzymatic hydrolysis is limited. A total of 5867 low-molecular weight peptides were identified in the highly bioactive subfractions of the PBBP alcalase hydrolysates using traditional and peptidomics approaches. 19 short peptides (3-5 amino acids) were identified by ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry, including 5 tripeptides, 8 tetrapeptides, and 6 pentapeptides. 5848 medium-sized peptides (6-10 amino acids) were characterized using the peptidomics approach, including 1484 hexapeptides, 1217 heptapeptides, 1634 octapeptides, 927 nonapeptides, and 586 decapeptides. The comprehensive method can be used for the investigation of bioactive peptides in complex food matrices.
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http://dx.doi.org/10.1021/acs.jafc.2c02487DOI Listing
July 2022

Phosphorylation of PBK/TOPK Tyr74 by JAK2 promotes Burkitt lymphoma tumor growth.

Cancer Lett 2022 Sep 30;544:215812. Epub 2022 Jun 30.

State Key Laboratory of Cancer Biology, Department of Pathology, Xijing Hospital and School of Basic Medicine, Air Force Medical University, Xi'an, China. Electronic address:

Burkitt lymphoma (BL), which is characterized by high invasiveness, is a subgroup of non-Hodgkin lymphoma. Although BL is regarded as a highly curable disease, especially for children, some patients unfortunately still do not respond adequately. The understanding of the etiology and molecular mechanisms of BL is still limited, and targeted therapies are still lacking. Here, we found that T-LAK cell-derived protein kinase (TOPK) and phosphorylated Janus kinase 2 (p-JAK2) are highly expressed in the tissues of BL patients. We report that TOPK directly binds to and is phosphorylated at Tyr74 by JAK2. Histone H3, one of the downstream targets of TOPK, is also phosphorylated in vivo and in vitro. Furthermore, we report that the phosphorylation of TOPK at Tyr74 by JAK2 plays a vital role in the proliferation of BL cells and promotes BL tumorigenesis in vivo. Phosphorylation of TOPK at Tyr74 by JAK2 enhances the stability of TOPK. Collectively, our results suggest that the JAK2/TOPK/histone H3 axis plays a key role in the proliferation of BL cells and BL tumorigenesis in vivo.
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http://dx.doi.org/10.1016/j.canlet.2022.215812DOI Listing
September 2022

Identification of Five Hub Genes Based on Single-Cell RNA Sequencing Data and Network Pharmacology in Patients With Acute Myocardial Infarction.

Front Public Health 2022 9;10:894129. Epub 2022 Jun 9.

Department of Cardiovascular Medicine, Shanghai University of Medicine and Health Sciences Affiliated Zhoupu Hospital, Shanghai, China.

Acute myocardial infarction (AMI) has a high mortality. The single-cell RNA sequencing (scRNA-seq) method was used to analyze disease heterogeneity at the single-cell level. From the Gene Expression Omnibus (GEO) database (GSE180678), AMI scRNA-seq were downloaded and preprocessed by the Seurat package. Gene expression data came from GSE182923. Cell cluster analysis was conducted. Cell types were identified. Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) analyses were performed on hub genes. Drugs were predicted by protein-protein interaction (PPI) and molecular docking. In total, 7 cell clusters were defined based on the scRNA-seq dataset, and the clusters were labeled as 5 cell types by marker genes. Hematopoietic stem cell types as a differential subgroups were higher in AMI than in healthy tissues. From available databases and PPI analysis, 52 common genets were identified. Based on 52 genes, 5 clusters were obtained using the MCODE algorithm, and genes in these 5 clusters involved in immune and inflammatory pathways were determined. Correlation analysis showed that hematopoietic stem cell types were negatively correlated with ATM, CARM1, and CASP8 but positively correlated with CASP3 and PPARG. This was reversed with immune cells. Molecular docking analysis showed that DB05490 had the lowest docking score with PPARG. We identified 5 hub genes (ATM, CARM1, CASP8, CASP3, and PPARG) involved in AMI progression. Compound DB05490 was a potential inhibitor of PPAG.
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http://dx.doi.org/10.3389/fpubh.2022.894129DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9219909PMC
June 2022

Integrated Design and Development of Intelligent Scenic Area Rural Tourism Information Service Based on Hybrid Cloud.

Comput Math Methods Med 2022 16;2022:5316304. Epub 2022 Jun 16.

Business School, Dalian University of Foreign Languages, Dalian 116044, China.

Although the "Internet+" technologies (big data and cloud computing) have been implemented in many industries, each industry involved in rural tourism economic information services has its own database, and there are still vast economic information resources that have not been exploited. Z travel agency through rural tourism enterprise third-party information services and mobile context-awareness-based Z travel has achieved good economic and social benefits by deep value mining and innovative application of the existing data of the enterprise through the third-party information service of rural tourism enterprises and mobile context-aware travel recommendation service. It clearly demonstrates that, in order to maximise the benefits of economic data, rural tourist businesses should focus not only on the application of new technologies and methodologies but also on the core of demand and data-driven and thoroughly investigate the potential value of current data. This paper mainly analyzes the problems related to how rural tourism can be upgraded under the smart tourism platform, with the aim of improving the development of China's rural tourism industry with the help of an integrated smart tourism platform, and proposes a hybrid cloud-based integrated system of smart scenic rural tourism information services, which can meet the actual use needs of rural tourism, with good shared service effect and platform application performance, and promote the development of rural tourism and resource utilization rate.
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http://dx.doi.org/10.1155/2022/5316304DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9225890PMC
June 2022

Application of a Convolutional Neural Network for Multitask Learning to Simultaneously Predict Microvascular Invasion and Vessels that Encapsulate Tumor Clusters in Hepatocellular Carcinoma.

Ann Surg Oncol 2022 Jun 26. Epub 2022 Jun 26.

Department of Hepatobiliary and Pancreatic Surgery, The First Hospital of Jilin University, Changchun, People's Republic of China.

Background: Hepatocellular carcinoma (HCC) is the fourth most common cause of cancer death worldwide, and the prognosis remains dismal. In this study, two pivotal factors, microvascular invasion (MVI) and vessels encapsulating tumor clusters (VETC) were preoperatively predicted simultaneously to assess prognosis.

Methods: A total of 133 HCC patients who underwent surgical resection and preoperative gadolinium ethoxybenzyl-diethylenetriaminepentaacetic acid (Gd-EOB-DTPA)-enhanced magnetic resonance imaging (MRI) were included. The statuses of MVI and VETC were obtained from the pathological report and CD34 immunohistochemistry, respectively. A three-dimensional convolutional neural network (3D CNN) for single-task learning aimed at MVI prediction and for multitask learning aimed at simultaneous prediction of MVI and VETC was established by using multiphase Gd-EOB-DTPA-enhanced MRI.

Results: The 3D CNN for single-task learning achieved an area under receiver operating characteristics curve (AUC) of 0.896 (95% CI: 0.797-0.994). Multitask learning with simultaneous extraction of MVI and VETC features improved the performance of MVI prediction, with an AUC value of 0.917 (95% CI: 0.825-1.000), and achieved an AUC value of 0.860 (95% CI: 0.728-0.993) for the VETC prediction. The multitask learning framework could stratify high- and low-risk groups regarding overall survival (p < 0.0001) and recurrence-free survival (p < 0.0001), revealing that patients with MVI+/VETC+ were associated with poor prognosis.

Conclusions: A deep learning framework based on 3D CNN for multitask learning to predict MVI and VETC simultaneously could improve the performance of MVI prediction while assessing the VETC status. This combined prediction can stratify prognosis and enable individualized prognostication in HCC patients before curative resection.
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http://dx.doi.org/10.1245/s10434-022-12000-6DOI Listing
June 2022

OX40 Expression in Eosinophils Aggravates OVA-Induced Eosinophilic Gastroenteritis.

Front Immunol 2022 2;13:841141. Epub 2022 Jun 2.

Department of Gastroenterology, Peking University Ninth School of Clinical Medicine, Beijing, China.

Background & Aims: Eosinophils are the main inflammatory effector cells that damage gastrointestinal tissue in eosinophilic gastrointestinal diseases (EGIDs). Activation of the OX40 pathway aggravates allergic diseases, such as asthma, but it is not clear whether OX40 is expressed in eosinophils to regulate inflammation in EGIDs. In this study, we assessed the expression and effect of OX40 on eosinophils in WT and eosinophilic gastroenteritis (EGE) mice.

Methods: Eosinophil infiltration, ovalbumin (OVA)-specific Ig production, OX40 expression and inflammatory factor levels in the intestine and bone marrow (BM) were investigated to evaluate inflammation.

Results: We confirmed that OVA-challenged mice produced high levels of mRNA and a low level of mRNA in the intestine. Increased eosinophils were observed in intestinal and lymph tissues, accompanied by significantly upregulated OX40 and Type 2 cytokine production in eosinophils of EGE mice. deficiency ameliorated OVA-induced inflammation, eosinophil infiltration, and cytokine production in the intestine. Consistently, eosinophils exhibited decreased proliferation and proinflammatory function. The stimulation of the agonistic anti-OX40 antibody, OX86, promoted the effect of OX40 on eosinophils. The present study also showed that deficiency dampened the Traf2/6-related NF-κB signaling pathway in eosinophils.

Conclusions: OX40 may play a critical role in the progress of OVA-induced EGE by promoting the maturation and function of eosinophils the Traf2/6-related NF-κB signaling pathway.
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http://dx.doi.org/10.3389/fimmu.2022.841141DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201343PMC
June 2022

Identification of a three-miRNA panel in serum for bladder cancer diagnosis by a diagnostic test.

Transl Cancer Res 2022 May;11(5):1005-1016

Department of Urology, Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Peking University Shenzhen Hospital, Clinical College of Anhui Medical University, Shenzhen, China.

Background: Bladder cancer (BC) is the tenth most common cancer in the world. Serum microRNA (miRNA) profiles previously have been reported as non-invasive biomarkers in cancer screening. The non-invasive and reliable diagnostic biomarkers are urgently needed for detecting BC, while cystoscopy is invasive. Our study aimed to identify candidate miRNAs in serum as potential diagnostic biomarkers for BC detection.

Methods: This study was including the screening stage, training stage, and validation stage with 137 BC patients and 127 healthy controls (HCs). We identified the expression of 28 serum miRNAs from 5 BC pools and 3 HC pools in the initial screening stage. The other 112 BC patients and 112 HCs were randomly divided into training stage with 30 BC patients and 30 HCs and validation stages with 82 BC patients and 82 HCs. These HCs matched BC patients based on age and gender with P value >0.05. Identified dysregulated miRNAs were further confirmed in the training stage, and validation stages by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The diagnostic value of miRNAs was assessed by receiver operating characteristic (ROC) curves and the area under the ROC curve (AUC). Target genes of 3 candidate miRNAs were predicted by bioinformatic analysis.

Results: Five miRNAs (miR-106a-5p, miR-145-5p, miR-132-3p, miR-7-5p and miR-148b-3p) in serum were obviously dysregulated in BC patients compared to HCs. The ability to diagnose BC of 3 candidate miRNAs was estimated by AUC, with miR-132-3p (AUC =0.781; sensitivity =68.29%, specificity =81.71%), miR-7-5p (AUC =0.778; sensitivity =59.76%, specificity =84.15%) and miR-148b-3p (AUC =0.837; sensitivity =81.71%, specificity =71.95%). Combined application of these candidate miRNAs with parallel test could improve the diagnostic value (AUC =0.922; sensitivity =90.24%, specificity =81.71%). , and , considered as target genes of the three-miRNA panel, may play an important role in the process of BC development.

Conclusions: A three-miRNA panel in serum was identified for BC diagnosis in our study, which HCs were used for differential diagnosis. The three-miRNA panel (miR-132-3p, miR-7-5p, and miR-148b-3p) might be performed as a non-invasive and convenient diagnostic tool for BC screening and diagnosis.
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http://dx.doi.org/10.21037/tcr-21-2611DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9189164PMC
May 2022

Nanoscale Colocalized Electrochemical and Structural Mapping of Metal Dissolution Reaction.

Anal Chem 2022 06 14;94(25):9058-9064. Epub 2022 Jun 14.

Department of Chemistry, The University of Texas at Austin, 105 East 24th Street, Austin, Texas 78712, United States.

Understanding the structure-activity relationship in electrochemical metal dissolution reactions is fundamentally important, from designing higher density batteries to mitigating corrosions. The kinetics of metal dissolution reaction is highly dependent on surface structures, including grain boundaries and local defects. However, directly probing the electrochemical activity at these sites is difficult because the conventional bulk electrochemistry measures an averaged kinetics, obscuring the structure-activity correlation. Herein, we report the colocalized mapping of an electrochemical metal dissolution reaction using Ag as a model system. The local dissolution kinetics is voltammetrically mapped via scanning electrochemical cell microscopy (SECCM), which is correlated with local structures obtained via colocalized electron backscattering diffraction (EBSD). Individual pits of ∼200 nm are formed, and their geometries suggest dissolution is fastest in the direction parallel to the {111} planes. Enhanced dissolution kinetics is observed at the high-angle grain boundaries but not at twin boundaries, which are attributed to the different binding energy of Ag atoms. Furthermore, the faster local dissolution correlates with the geometrically necessary dislocation density. The work demonstrates the importance of nanoscale local electrochemical mapping and colocalized microscopic measurement in obtaining the structure-activity relationship for electrochemical reactions at complex interfaces.
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http://dx.doi.org/10.1021/acs.analchem.2c01283DOI Listing
June 2022

Experimental analysis of soil moisture response to rainfall in a typical grassland hillslope under different vegetation treatments.

Environ Res 2022 Oct 7;213:113608. Epub 2022 Jun 7.

The College of Water Conservancy and Civil Engineering Inner Mongolia Agricultural University, Hohhot, 010018, China.

The responses of soil moisture to rainfall are of great significance for watershed hydrological modeling. However, few studies have been done to investigate these responds on hillslope in a typical semi-arid grassland region. This study used high temporal resolution soil moisture data to explore the soil moisture dynamics, response conditions and its controls of 0-40 cm soil profile in the upslope (14°), midslope (9°), and downslope (4°) of a typical grassland inland river basin under bare ground (BG), stubble (SG), and natural grassland (CK) treatments. The results showed that soil water content and water storage increased in the downslope direction, and all showed as BG > SG > CK. The dry and wet changes in fast-changing layer (5 cm) and active layer (10 cm) were rapid, while soil moisture below 20 cm was relatively stable and fluctuated only in heavy or continuous rainfalls. The soil moisture response process varied greatly under different rainfall, rainfall intensity and antecedent soil moisture conditions, which explained 41.1% of the total difference. The rainfall replenishment threshold and the required initial soil profile water content of soil moisture response in 5 cm, 10 cm and 20 cm soil layers were 5.8 mm, 8.0 mm, 11.4 mm and 8.7 vol%, 9.4 vol%, 10.8 vol%, respectively. Soil properties, vegetation characteristics and topography could explain 38.8%, 14.5% and 5.6% of the soil moisture variation on the hillslope. In addition, under the comprehensive influence of environmental factors, changes in soil moisture of the upslope were significantly affected by soil sand content, the differences in the midslope were mainly due to soil clay content and belowground biomass, whereas the vegetation characteristics were the main factors in the downslope. This study can contribute to the further understanding of slope-scale ecohydrological processes and hydrological simulation of semi-arid grassland watersheds.
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http://dx.doi.org/10.1016/j.envres.2022.113608DOI Listing
October 2022

The effect of extensor muscle strength on meniscus damage progression in subjects without radiologic knee osteoarthritis - Data from the Osteoarthritis Initiative.

Am J Phys Med Rehabil 2021 Nov 15. Epub 2021 Nov 15.

Department of Orthopedics, Orthopedic Research Institute, West China Hospital, Sichuan University, Chengdu, Sichuan Province, China.

Background: It has been demonstrated that high extensor strength decreases knee osteoarthritis (KOA) symptomatic progression. However, few studies have detected a significant association between extensor strength and structural progression.

Methods: Participants in the Osteoarthritis Initiative with both muscle strength and meniscus assessment, KL grade <= 1, and BMI < 30 were enrolled. In a separate-sex analysis, participants were divided into the high strength and low strength groups, referring to the median value. Meniscus progression according to the MRI Osteoarthritis Knee Score was compared between the two groups at 12 months (393 females and 229 males) and 24 months (340 females and 208 males).

Results: In females, less overall medial meniscus progression (11.1% [17/153] vs. 23.2% [32/138], P = 0.04), less medial meniscal medial extrusion (5.2% [8/155] vs. 12.5% [18/144], P = 0.04), and less medial meniscal anterior extrusion progression (0% [0/108] vs. 5.3% [6/113], P = 0.03) were present in the high strength group at 24 months. In males, no significant difference was detected between the high strength group and the low strength group.

Conclusion: In females, higher extensor muscle strength was associated with a decreased risk of medial meniscus progression in medial and anterior extrusion.
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http://dx.doi.org/10.1097/PHM.0000000000001922DOI Listing
November 2021

Intracellular Lipid Accumulation Drives the Differentiation of Decidual Polymorphonuclear Myeloid-Derived Suppressor Cells via Arachidonic Acid Metabolism.

Front Immunol 2022 18;13:868669. Epub 2022 May 18.

Department of Obstetrics and Gynecology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Decidual polymorphonuclear myeloid-derived suppressor cells (PMN-MDSCs) are essential to immune tolerance during pregnancy. A reduction in the number of these cells is associated with unexplained recurrent pregnancy loss (URPL). In our previous study, we reported that PMN-MDSCs are a group of mature neutrophils that are activated by the decidua microenvironment. In the present study, we show that the decidua microenvironment induces substantial lipid accumulation in neutrophils during their differentiation to PMN-MDSCs. Lower levels of lipid accumulation are detected in PMN-MDSCs from URPL patients, and the amount of lipid in the PMN-MDSCs is positively correlated with the proportion of PMN-MDSCs. Next, we demonstrate that decidua-derived IL6 with the presence of arachidonic acid upregulates fatty acid-binding protein 5 (FABP5) the phosphorylation of signal transducer and activator of transcription 3 (STAT3). Fy -60ABP5 then continuously stimulates intracellular lipid accumulation. Increased intracellular lipid accumulation mediates arachidonic acid metabolism, a pathway that is significantly activated by the induction of the decidua microenvironment, to stimulate the synthesis of prostaglandin E2 (PGE2) and finally induce the differentiation of PMN-MDSCs. To summarize, decidua-derived IL6 facilitates the differentiation of PMN-MDSCs from neutrophils the pSTAT3/FABP5/PGE2 pathway. Defects in the process may result in impaired differentiation and dysfunction of PMN-MDSCs in URPL. These findings enhance our understanding of the physiological mechanisms of immune tolerance in pregnancy and provide therapeutic options for URPL.
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http://dx.doi.org/10.3389/fimmu.2022.868669DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9159278PMC
May 2022

A genetic correlation scan identifies blood proteins associated with bone mineral density.

BMC Musculoskelet Disord 2022 Jun 3;23(1):530. Epub 2022 Jun 3.

Orthopedic Research Institute, Department of Orthopedics, Sichuan University West China Hospital, 37# Guoxue Road, Chengdu, 610041, Sichuan Province, PR China.

Background: Osteoporosis is a common metabolic bone disease that is characterized by low bone mass. However, limited efforts have been made to explore the functional relevance of the blood proteome to bone mineral density across different life stages.

Methods: Using genome-wide association study summary data of the blood proteome and two independent studies of bone mineral density, we conducted a genetic correlation scan of bone mineral density and the blood proteome. Linkage disequilibrium score regression analysis was conducted to assess genetic correlations between each of the 3283 plasma proteins and bone mineral density.

Results: Linkage disequilibrium score regression identified 18 plasma proteins showing genetic correlation signals with bone mineral density in the TB-BMD cohort, such as MYOM2 (coefficient = 0.3755, P value = 0.0328) among subjects aged 0 ~ 15, POSTN (coefficient = - 0.5694, P value = 0.0192) among subjects aged 30 ~ 45 and PARK7 (coefficient = - 0.3613, P value = 0.0052) among subjects aged over 60.

Conclusions: Our results identified multiple plasma proteins associated with bone mineral density and provided novel clues for revealing the functional relevance of plasma proteins to bone mineral density.
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http://dx.doi.org/10.1186/s12891-022-05453-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9164489PMC
June 2022

Selective Photocatalytic CO Reduction to CH on Tri--triazine-Based Carbon Nitride via Defects and Crystal Regulation: Synergistic Effect of Thermodynamics and Kinetics.

ACS Appl Mater Interfaces 2022 Jun 30;14(22):25417-25426. Epub 2022 May 30.

Shanghai Engineering Research Center for Multi-Media Environmental Catalysis and Resource Utilization, Key Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering, Feringa Nobel Prize Scientist Joint Research Center, Frontiers Science Center for Materiobiology and Dynamic Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, P. R. China.

Realizing the high selectivity of CH from the photocatalytic CO reduction reaction (CO RR) remains a great challenge owing to the lower efficiency of multi-electron transfer and the similar thermodynamic properties of CH and CO. Herein, nitrogen-deficient carbon nitride two-dimensional (2D) nanosheets were prepared via the high-temperature crystalline phase transformation process. Optimizing crystallinity enhances the in-plane polarization along the -axis. Owing to the increased electron density of the N defect, the kinetic possibilities of CH production have increased. Furthermore, the potential energy of the mid-gap states introduced by the N defect favors the thermodynamics of CH production. The selectivity values of CH based on yield and electrons are 87.1 and 96.4%. This work unravels the mechanism to selectively produce CH from CO photoreduction through the crystalline phase and defect regulation and provides significant guidance for the rational design of CO reduction photocatalysts for selective CH production.
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http://dx.doi.org/10.1021/acsami.2c03913DOI Listing
June 2022

A Salt-Tolerant D2-8 from Rhizosphere Soil of Augments Soybean Tolerance to Soda Saline-Alkali Stress.

Pol J Microbiol 2022 Mar 14;71(1):43-53. Epub 2022 Mar 14.

College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing, China.

Soil salinity and alkalization limit plant growth and agricultural productivity worldwide. The application of salt-tolerant plant growth-promoting rhizobacteria (PGPR) effectively improved plant tolerance to saline-alkali stress. To obtain the beneficial actinomyces resources with salt tolerance, thirteen isolates were isolated from rhizosphere saline and alkaline soil of . Among these isolates, D2-8 was moderately halophilic to NaCl and showed 120 mmol soda saline-alkali solution tolerance. Moreover, the plant growth-promoting test demonstrated that D2-8 produced siderophore, IAA, 1-aminocyclopropane-1-carboxylate deaminase (ACCD), and organic acids. D2-8 showed 99.4% homology with the type strain NBRC 14887 and shared the same branch, and, therefore, it was designated . D2-8. Its genome was sequenced to gain insight into the mechanism of growth-promoting and saline-alkali tolerance of D2-8. IAA and siderophore biosynthesis pathway, genes encoding ACC deaminase, together with six antibiotics biosynthesis gene clusters with antifungal or antibacterial activity, were identified. The compatible solute ectoine biosynthesis gene cluster, production, and uptake of choline and glycine betaine cluster in the D2-8 genome may contribute to the saline-alkali tolerance of the strain. Furthermore, D2-8 significantly promoted the seedling growth even under soda saline-alkali stress, and seed coating with D2-8 isolate increased by 5.88% of the soybean yield in the field. These results imply its significant potential to improve soybean soda saline-alkali tolerance and promote crop health in alkaline soil.
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http://dx.doi.org/10.33073/pjm-2022-006DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9152913PMC
March 2022

Photocatalytic Removal of Cr(VI) by Thiourea Modified Sodium Alginate/Biochar Composite Gel.

Gels 2022 May 9;8(5). Epub 2022 May 9.

Anhui Province Key Laboratory of Metallurgical Engineering & Resources Recycling, Anhui University of Technology, Maanshan 243002, China.

Heavy metal pollution is an important problem in current water treatments. Traditional methods for treating chromium-containing wastewater have limitations such as having complicated processes and causing secondary pollution. Therefore, seeking efficient and fast processing methods is an important research topic at present. Photocatalysis is an efficient method to remove Cr(VI) from aqueous solutions; however, conventional photocatalysts suffer from a low metal absorption capacity, high investment cost, and slow desorption of trivalent chromium from the catalyst surface. In this study, a novel composite gel was synthesized by chemically modifying thiourea onto sodium alginate, which was then mixed with biochar. The composite gel (T-BSA) can effectively remove 99.98% of Cr(VI) in aqueous solution through synergistic adsorption and photocatalytic reduction under UV light irradiation. The removal mechanism of Cr(VI) was analyzed by FT-IR, FESEM, UV-DRS and XPS. The results show that under acidic conditions, the amino group introduced by chemical modification can be protonated to adsorb Cr(VI) through electrostatic interaction. In addition, the biochar as a functional material has a large specific surface area and pore structure, which can provide active sites for the adsorption of Cr(VI), while the photo-reduced Cr(III) is released into the solution through electrostatic repulsion, regenerating the adsorption sites, thereby improving the removal performance of Cr(VI). Biochar significantly intensifies the Cr(VI) removal performance by providing a porous structure and transferring electrons during photoreduction. This study demonstrates that polysaccharide-derived materials can serve as efficient photocatalysts for wastewater treatment.
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http://dx.doi.org/10.3390/gels8050293DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9141709PMC
May 2022

Rapid detection method of Skeletonema pseudocostatum and preparation of test strip.

Environ Sci Pollut Res Int 2022 May 18. Epub 2022 May 18.

Key Laboratory of Ministry of Education for Coastal and Wetland Ecosystems, College of the Environment and Ecology, Xiamen University, Xiamen, 361102, Fujian, China.

In eutrophic waters, harmful algal blooms (HAB) are particularly prone to occur, which will affect the ecological environment and public health and safety. How to quickly detect and monitor marine microalgae is the key to preventing and managing HAB. Our innovative application of colloidal gold immunochromatography (GICG) technology to detect the dominant species in red tide, Skeletonema pseudocostatum, to monitor the outbreak of red tide. The experimental results show that the method and the prepared test strips are extremely sensitive and can specifically detect the presence of Skeletonema pseudocostatum. The approximate concentration of algae cells is judged by establishing a fitting relationship between the degree of color development and the concentration of algae cells. This test strip provides a quick and easy method for routine environmental monitoring, fishery water quality monitoring, and field testing of red tide monitoring. It effectively warns of the outbreak of red tides and also provides a new application direction for GICG technology.
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http://dx.doi.org/10.1007/s11356-022-20040-4DOI Listing
May 2022

Spatiotemporal hysteresis distribution and decomposition of solar activities and climatic oscillation during 1900-2020.

Environ Res 2022 09 14;212(Pt C):113435. Epub 2022 May 14.

Department of Biological and Agricultural Engineering & Zachry Department of Civil Engineering, Texas A& M University, College Station, TX, 77843, USA.

Solar radiation is the external driving force of the Earth's climate system. In different spatial and temporal scales, meteorological elements have different responses and lag periods to solar activity (SA), climatic oscillation (CO), geographic factors (GF) and other influencing factors. However, such studies are not abundant and in-depth in the world. To further understand the "solar-climate-water resource" system, this study considers China as the study area and investigates the monthly data of temperature (T) and precipitation (P) during 1900-2020 that were obtained from 3836 grid stations. The strong interaction and lag distribution between T or P with SA and CO were studied and influence weights of SA, CO, and geographical factors (GF) of each grid station were calculated. A multivariate hysteretic decomposition model was established to simulate and quantitatively decompose the periodic lag considering the factors of the earth's revolution. It is found that the strong interaction/lag periods obtained in a long-time scale can be decomposed into several periods shorter than the SA period. The distribution of strong interaction/lag periods is nested with topography and echoes with cities. The underlying surface conditions and urbanization are also important factors affecting the T and P lag. There are two distinct dividing lines in the lag period and influencing factor pattern of T and P. The T dividing line moves through valleys where water or mountain ranges meet, where the gap facilitates monsoon movement across regions, while the P dividing line is a zone of dramatic terrain, where tall mountains block water vapor transport. In the lag trend of T, the northern region of China has the longest lag period, and the lag period of surrounding regions tends to converge to the northern region. The lag period caused by SN in southwest China is larger than that in northwest China, while the lag effect of CO is opposite in the above two regions. The lag trend of P also has the above characteristics, but the difference is that the lag period in central China is the longest.
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http://dx.doi.org/10.1016/j.envres.2022.113435DOI Listing
September 2022

SIRT5 is involved in the proliferation and metastasis of breast cancer by promoting aerobic glycolysis.

Pathol Res Pract 2022 Jul 11;235:153943. Epub 2022 May 11.

State Key Laboratory of Cancer Biology, Department of Pathology, Xijing Hospital and School of Basic Medicine, Fourth Military Medical University, Xi'an, China. Electronic address:

Objective: Breast cancer (BC) is the most commonly diagnosed cancer among females and has a poor prognosis, breast invasive ductal carcinoma is the most common histological type. The occurrence and development of BC is closely related to aberrant glucose metabolism. In the hyperglycemic environment caused by abnormal glucose metabolism, hypoxia-inducible factor-1 alpha (HIF-1α) enables tumor cells to absorb large amounts of glucose and enhance glycolysis by inducing the expression of glucose transporter type1 (GLUT1) and glycolysis genes, thus promoting tumor cell proliferation and metastasis. Mitochondrial Sirtuin5 (SIRT5) plays a role in the rewiring of glucose metabolism during the progression of cancers. Thus, we aimed to elucidate whether SIRT5 promotes BC proliferation and metastasis by facilitating aerobic glycolysis in BC.

Methods: The expression of SIRT5 in breast carcinoma tissue and cells was evaluated using immunohistochemical staining, western blot and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis to confirm the biological role of SIRT5 in breast carcinoma. We established a stable cell line with SIRT5 knockdown using lentiviral transduction in T47D cells to reduce SIRT5 expression and then evaluated the effect of SIRT5 on cells cultured in the presence of high glucose (4500 mg/L) and normal glucose (2000 mg/L) concentrations. Cell proliferation was detected using the CCK-8 assay, the cell cycle and cell apoptosis were measured using flow cytometry and Annexin V staining, and cell migration was tested by performing Celigo scratch and Transwell assays. The expression of PKM2, HK2, mTOR and HIF-1α, which play roles in aerobic glycolysis, was investigated using western blot.

Results: SIRT5 was overexpressed in BC tissues compared with paired normal tissues. Prognostic and OS analyses showed that the SIRT5 expression level was an individual prognostic factor for patients with BC. SIRT5 knockdown inhibited proliferation and metastasis and slightly increased apoptosis in T47D cells under high-glucose conditions. Furthermore, the downregulation of HK2 and HIF-1α caused by SIRT5 knockdown was a high glucose-dependent process, while the downregulation of PKM2 was mediated by a high glucose-independent process.

Conclusions: SIRT5 is an independent prognostic factor for BC and contributes to cell proliferation and metastasis in a high glucose-dependent manner to some degree, which might be mediated by promoting aerobic glycolysis.
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http://dx.doi.org/10.1016/j.prp.2022.153943DOI Listing
July 2022

Prognostic Characteristics and Immune Effects of N-Methyladenosine and 5-Methylcytosine-Related Regulatory Factors in Clear Cell Renal Cell Carcinoma.

Front Genet 2022 27;13:864383. Epub 2022 Apr 27.

Department of Urology, Shengjing Hospital of China Medical University, Shenyang, China.

In recent years, methylation modification regulators have been found to have essential roles in various tumor mechanisms. However, the relationships between N-methyladenosine (m6A) and 5-methylcytosine (m5C) regulators and clear cell renal cell carcinoma (ccRCC) remain unknown. This study investigated these relationships using the data from The Cancer Genome Atlas database. We calculated risk scores using a Lasso regression analysis and divided the patient samples into two risk groups (tumor vs. normal tissues). Furthermore, we used univariate and multivariate Cox analyses to determine independent prognostic indicators and explore correlations between the regulatory factors and immune infiltrating cell characteristics. Finally, quantitative reverse transcriptase-polymerase chain reaction (PCR) and The Human Protein Atlas were used to verify signature-related gene expression in clinical samples. We identified expression differences in 35 regulatory factors between the tumor and normal tissue groups. Next, we constructed a five-gene risk score signature (NOP2 nucleolar protein [], methyltransferase 14, N6-adenosine-methyltransferase subunit [], NOP2/Sun RNA methyltransferase 5 [], heterogeneous nuclear ribonucleoprotein A2/B1 [], and zinc finger CCCH-type containing 13 []) using the screening criteria ( < 0.01), and then divided the cases into high- and low-risk groups based on their median risk score. We also screened for independent prognostic factors related to age, tumor grade, and risk score. Furthermore, we constructed a Norman diagram prognostic model by combining two clinicopathological characteristics, which demonstrated good prediction efficiency with prognostic markers. Then, we used a single-sample gene set enrichment analysis and the cell-type identification by estimating relative subsets of RNA transcripts (CIBERSORT) method to evaluate the tumor microenvironment of the regulatory factor prognostic characteristics. Moreover, we evaluated five risk subgroups with different genetic signatures for personalized prognoses. Finally, we analyzed the immunotherapy and immune infiltration response and demonstrated that the high-risk group was more sensitive to immunotherapy than the low-risk group. The PCR results showed that and expression was higher in tumor tissues than in normal tissues. In conclusion, we identified five m6A and m5C regulatory factors that might be promising biomarkers for future research.
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http://dx.doi.org/10.3389/fgene.2022.864383DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9092148PMC
April 2022

5-methylcytosine RNA methyltransferases and their potential roles in cancer.

J Transl Med 2022 05 13;20(1):214. Epub 2022 May 13.

Department of Urology, Shengjing Hospital of China Medical University, No. 36 Sanhao Street, Heping District, Shenyang, 110004, Liaoning, People's Republic of China.

In recent years, 5-methylcytosine (mC) RNA modification has emerged as a key player in regulating RNA metabolism and function through coding as well as non-coding RNAs. Accumulating evidence has shown that mC modulates the stability, translation, transcription, nuclear export, and cleavage of RNAs to mediate cell proliferation, differentiation, apoptosis, stress responses, and other biological functions. In humans, mC RNA modification is catalyzed by the NOL1/NOP2/sun (NSUN) family and DNA methyltransferase 2 (DNMT2). These RNA modifiers regulate the expression of multiple oncogenes such as fizzy-related-1, forkhead box protein C2, Grb associated-binding protein 2, and TEA domain transcription factor 1, facilitating the pathogenesis and progression of cancers. Furthermore, the aberrant expression of methyltransferases have been identified in various cancers and used to predict the prognosis of patients. In this review, we present a comprehensive overview of mC RNA methyltransferases. We specifically highlight the potential mechanism of action of mC in cancer. Finally, we discuss the prospect of mC-relative studies.
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http://dx.doi.org/10.1186/s12967-022-03427-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9102922PMC
May 2022
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