Publications by authors named "Mingxia Jiang"

20 Publications

  • Page 1 of 1

Dihydroartemisinin mediating PKM2-caspase-8/3-GSDME axis for pyroptosis in esophageal squamous cell carcinoma.

Chem Biol Interact 2021 Oct 13:109704. Epub 2021 Oct 13.

Department of Colorectal Surgery, Harbin Medical University Cancer Hospital, Harbin, 150081, China. Electronic address:

Pyroptosis is a novel type of pro-inflammatory programmed cell death that has been strongly reported to be related to inflammation, immune, and cancer. Dihydroartemisinin (DHA) has good anti-tumor properties. However, the exact mechanism by which DHA induces pyroptosis to inhibit esophageal squamous cell carcinoma (ESCC) remains unclear. After applying DHA treatment to ESCC, we found that some dying cells exhibited the characteristic morphology of pyroptosis, such as blowing large bubbles from the cell membrane, accompanied by downregulation of pyruvate kinase isoform M2 (PKM2), activation of caspase-8/3, and production of GSDME-NT. Meanwhile, it was accompanied by an increased release of LDH and inflammatory factors (IL-18 and IL-1β). Both knockdown of GSDME and application of caspase-8/3 specific inhibitors (z-ITED-FMK/Ac-DEVD-CHO) significantly inhibited DHA-induced pyroptosis. However, the former did not affect the activation of caspase-3. In contrast, overexpression of PKM2 inhibited caspase-8/3 activation as well as GSDME-N production. Furthermore, both si-GSDME and OE-PKM2 inhibited DHA-induced pyroptosis in vivo and in vitro. Therefore, the results suggest that DHA can induce pyroptosis of ESCC cells via the PKM2-caspase-8/3-GSDME pathway. Implication: In this study, we identified new mechanism of DHA in inhibiting ESCC development and progression, and provide a potential therapeutic agent for the treatment of ESCC.
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http://dx.doi.org/10.1016/j.cbi.2021.109704DOI Listing
October 2021

Chemotherapeutic drug-induced immunogenic cell death for nanomedicine-based cancer chemo-immunotherapy.

Nanoscale 2021 Oct 13. Epub 2021 Oct 13.

College of Pharmacy, Weifang Medical University, Weifang 261053, China.

Chemotherapy has been a conventional paradigm for cancer treatment, and multifarious chemotherapeutic drugs have been widely employed for decades with significant performances in suppressing tumors. Moreover, some of the antitumor chemotherapeutic agents, such as doxorubicin (DOX), oxaliplatin (OXA), cyclophosphamide (CPA) and paclitaxel (PTX), can also tackle tumors through the induction of immunogenic cell death (ICD) in tumor cells to trigger specific antitumor immune responses of the body and improve chemotherapy efficacy. In recent years, chemo-immunotherapy has attracted increasing attention as one of the most promising combination therapies to struggle with malignant tumors. Many effective antitumor therapies have benefited from the successful induction of ICD in tumors, which could incur the release of endogenous danger signals and tumor-associated antigens (TAAs), further stimulating antigen-presenting cells (APCs) and ultimately initiating efficient antitumor immunity. In this review, several well-characterized damage-associated molecular patterns (DAMPs) were introduced and the progress of ICD induced by representative chemotherapeutic drugs for nanomedicine-based chemo-immunotherapy was highlighted. In addition, the combination strategies involving ICD cooperated with other therapies were discussed. Finally, we shared some perspectives in chemotherapeutic drug-induced ICD for future chemo-immunotherapy. It was hoped that this review would provide worthwhile presentations and enlightenments for cancer chemo-immunotherapy.
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http://dx.doi.org/10.1039/d1nr05512gDOI Listing
October 2021

Sequentially pH-Responsive Drug-Delivery Nanosystem for Tumor Immunogenic Cell Death and Cooperating with Immune Checkpoint Blockade for Efficient Cancer Chemoimmunotherapy.

ACS Appl Mater Interfaces 2021 Sep 10;13(37):43963-43974. Epub 2021 Sep 10.

College of Pharmacy, Weifang Medical University, Weifang 261053, China.

Chemoimmunotherapy has anchored a new blueprint for cancer management. As a burgeoning approach, immunotherapy has shifted the paradigm of traditional chemotherapy and opened up new prospects for cancer treatment. Here, a sequentially pH-responsive doxorubicin (DOX) delivery nanosystem is designed for simultaneous chemotherapy and tumor immunogenic cell death (ICD). DOX is modified into pH-sensitive -aconityl-doxorubicin (CAD) for being easily adsorbed by polycationic polyethylenimine (PEI), and the PEI/CAD complexes are in situ-shielded by aldehyde-modified polyethylene glycol (PEG). The PEG/PEI/CAD nanoparticles (NPs) can keep stable in neutral physiological pH during systemic circulation but will detach PEG shielding once in slightly acidic tumor extracellular pH. The exposed positive PEI/CAD complexes are endocytosed effortlessly, and CAD is then converted back to DOX by endosomal-acidity-triggered -aconityl cleavage. The released DOX further elicits ICD, and the moribund tumor cells will release antigens and damage-associated molecular patterns to recruit dendritic cells and activate antitumor immunity. An excellent therapeutic effect is achieved when the immune checkpoint PD-1 antibody (aPD-1) is utilized to cooperate with the PEG/PEI/CAD NPs for blocking tumor immune escape and maintaining antitumor activity of the ICD-instigated T cells. The sequentially pH-responsive DOX delivery nanosystem cooperating with immune checkpoint blockade will provide a potential strategy for cancer chemoimmunotherapy.
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http://dx.doi.org/10.1021/acsami.1c10643DOI Listing
September 2021

CpG-Based Nanovaccines for Cancer Immunotherapy.

Int J Nanomedicine 2021 5;16:5281-5299. Epub 2021 Aug 5.

College of Pharmacy, Weifang Medical University, Weifang, 261053, People's Republic of China.

Cancer has been a serious health hazard to the people all over the world with its high incidence and horrible mortality. In recent years, tumor vaccines in immunotherapy have become a hotspot in cancer therapy due to their many practical advantages and good therapeutic potentials. Among the various vaccines, nanovaccine utilized nanoparticles (NPs) as the carrier and/or adjuvant has presented significant therapeutic effect in cancer treatment. For tumor nanovaccines, unmethylated cytosine-phosphate-guanine oligodeoxynucleotide (CpG ODN) is a commonly used adjuvant. It has been reported that CpG ODN was the most effective immune stimulant among the currently known adjuvants. It could be recognized by toll-like receptor 9 (TLR9) to activate humoral and cellular immunity for preventing or treating cancer. In this review, the topic of CpG-based nanovaccines for cancer immunotherapy will be focused. The types and properties of different CpG will be introduced in detail first, and then some representative tumor nanovaccines will be reviewed according to the diverse loading modes of CpG, such as electrostatic adsorption, covalent bonding, hydrophilic and hydrophobic interaction, and DNA self-assembly, for summarizing the current progress of CpG-based tumor nanovaccines. Finally, the challenges and future perspectives will be discussed. It is hoped that this review will provide valuable references for the development of nanovaccines in cancer immunotherapy.
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http://dx.doi.org/10.2147/IJN.S317626DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8352601PMC
August 2021

Selective thermotherapy of tumor by self-regulating photothermal conversion system.

J Colloid Interface Sci 2022 Jan 30;605:752-765. Epub 2021 Jul 30.

College of Pharmacy, Weifang Medical University, Weifang 261053, China; Collaborative Innovation Center for Target Drug Delivery System, Weifang Medical University, Weifang 261053, China; Shandong Engineering Research Center for Smart Materials and Regenerative Medicine, Weifang Medical University, Weifang 261053, China. Electronic address:

One major challenge of photothermal therapy (PTT) is achieving thermal ablation of the tumor without damaging the normal cells and tissues. Here, we designed a self-regulating photothermal conversion system for selective thermotherapy based on self-assembling gold nanoparticles (S-AuNPs) and investigated the selectivity effect using a novel home-made in vitro selective photothermal transformation model and an in vivo skin damaging assessment model. In the in vitro selective photothermal transformation model, laser irradiation selectively increased the temperature of the internal microenvironment (pH 5.5) and resulted in an obvious temperature difference (ΔT ≥ 5 °C) with that of the external environment (pH 7.4). More importantly, in the in vivo skin damaging assessment model, S-AuNPs achieved good tumor inhibition without damaging the normal skin tissue compared with the conventional photothermal material. This work provides not only a novel validation protocol for tumor thermotherapy to achieve the biosafety of specifically killing tumor cells and normal tissue but also an evaluation methodology for other precise therapy for cancers.
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http://dx.doi.org/10.1016/j.jcis.2021.07.134DOI Listing
January 2022

eEF2K as a novel metastatic and prognostic biomarker in gastric cancer patients.

Pathol Res Pract 2021 Sep 29;225:153568. Epub 2021 Jul 29.

Department of Colorectal Surgery, Harbin Medical University Cancer Hospital, Harbin 150081, China. Electronic address:

Background: Although eukaryotic elongation factor 2 kinase (eEF2K) has been reported to be a potential oncogenic factor in many human cancers, its usefulness as a clinical prognostic biomarker for gastric cancer has not been investigated.

Methods: In this study, data about 540 patients with stomach adenocarcinoma (STAD) were analyzed from The Cancer Genome Atlas (TCGA) and the Gene Expression Omnibus (GEO) databases to determine the expression of eEF2K. Immunohistochemistry (IHC), western blots, and real-time polymerase chain reaction (RT-PCR) were also performed to determine the clinical significance of eEF2K expression in 96 postoperative patients with gastric cancer. Among the 96 patients, 36 had low expression of eEF2K and 60 had high expression.

Results: Analysis of the TCGA and GEO datasets revealed that eEF2K expression was significantly higher in the STAD tissue samples than in the non-tumorous gastric tissues. IHC, western blots, and RT-PCR confirmed these findings. The high expression level of eEF2K was found to be related to the presence of lymph node metastasis (p = 0.002). Moreover, multivariate analysis showed that eEF2K was an independent indicator of prognosis for overall survival (OS) (hazard ratio [HR] = 1.72, 95% confidence interval [CI] = 1.06-2.79; p = 0.03) and disease-free survival (DFS) (HR = 1.66, 95% CI = 0.997-2.765; p = 0.052) in patients with surgically resected STAD.

Conclusion: Collectively, our findings suggest that eEF2K is a clinical indicator of metastatic and prognostic significance for STAD survival and could serve as a potential therapeutic target.
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http://dx.doi.org/10.1016/j.prp.2021.153568DOI Listing
September 2021

Bilayer Membrane Composed of Mineralized Collagen and Chitosan Cast Film Coated With Berberine-Loaded PCL/PVP Electrospun Nanofiber Promotes Bone Regeneration.

Front Bioeng Biotechnol 2021 19;9:684335. Epub 2021 Jul 19.

Department of Microbiology, Weifang Medical University, Weifang, China.

Bone defects are difficult to repair and reconstruct as bone regeneration remains technically challenging, with exogenous factors required to accelerate this process. Biodegradable synthetic scaffolds are promising materials for stimulating bone tissue repair. In this study, we investigated whether a bilayer membrane that includes mineralized collagen (MC) and chitosan (CS) delivering berberine (BER)-a typical Chinese herbal monomer-could promote bone healing in a rat model. An MC/CS cast film was coated with polycaprolactone (PCL)/polyvinylpyrrolidone (PVP) electrospun nanofibers loaded with BER, yielding the [email protected]/PVP-MC/CS bilayer membrane. The 3-dimensional structure had nanofibers of uniform diameter and showed good hydrophilicity; the bilayer membrane showed favorable mechanical properties. [email protected]/PVP-MC/CS enhanced the proliferation and attachment of MC3T3-E1 cells and induced bone regeneration when implanted into a rat femoral bone defect. These findings provide evidence that [email protected]/PVP-MC/CS has clinical potential for effective bone repair.
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http://dx.doi.org/10.3389/fbioe.2021.684335DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8327095PMC
July 2021

Photodynamic therapy induces human esophageal carcinoma cell pyroptosis by targeting the PKM2/caspase-8/caspase-3/GSDME axis.

Cancer Lett 2021 Nov 10;520:143-159. Epub 2021 Jul 10.

Department of Gastrointestinal Oncology, Harbin Medical University Cancer Hospital, 150 Haping St, Nangang District, Harbin, Heilongjiang, 150081, PR China. Electronic address:

Photodynamic therapy (PDT) uses a photosensitizer (PS) and visible light to induce cancer cell death. Pyroptosis is a new type of programmed cell death that is associated with the gasdermin protein family. However, the precise mechanism of pyroptosis in PDT-induced suppression of esophageal cancer remains unknown. We demonstrate that PDT can induce gasdermin E (GSDME)-mediated pyroptosis, which is characterized by the formation of pyroptotic blebs in esophageal squamous cell carcinoma (ESCC), which burst and release intracellular contents and pro-inflammatory mediators. Mechanistically, PDT may inhibit pyruvate kinase M2 (PKM2) and consequently, activate caspase-8 and caspase-3, which ultimately releases N-GSDME and triggers pyroptosis in ESCC. Moreover, PDT decreased the efficiency of pyroptosis in the presence of a glycolytic inhibitor. Overall, our results show that PDT induces pyroptosis in ESCC by targeting the PKM2/caspase-8/caspase-3/GSDME axis. This is the first in-depth study of the specific mechanism underlying PKM2-mediated pyroptosis under PDT in ESCC, and potentially has great implications for the clinical application of PDT in ESCC.
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http://dx.doi.org/10.1016/j.canlet.2021.07.014DOI Listing
November 2021

Pyroptosis, a new bridge to tumor immunity.

Cancer Sci 2021 Oct 10;112(10):3979-3994. Epub 2021 Aug 10.

Department of Gastrointestinal Oncology, Harbin Medical University Cancer Hospital, Harbin, China.

Pyroptosis refers to the process of gasdermin (GSDM)-mediated programmed cell death (PCD). Our understanding of pyroptosis has expanded beyond cells and is known to involve extracellular responses. Recently, there has been an increasing interest in pyroptosis due to its emerging role in activating the immune system. In the meantime, pyroptosis-mediated therapies, which use the immune response to kill cancer cells, have also achieved notable success in a clinical setting. In this review, we discuss that the immune response induced by pyroptosis activation is a double-edged sword that affects all stages of tumorigenesis. On the one hand, the activation of inflammasome-mediated pyroptosis and the release of pyroptosis-produced cytokines alter the immune microenvironment and promote the development of tumors by evading immune surveillance. On the other hand, pyroptosis-produced cytokines can also collect immune cells and ignite the immune system to improve the efficiency of tumor immunotherapies. Pyroptosis is also related to some immune checkpoints, especially programmed death-1 (PD-1) or programmed death- ligand 1 (PD-L1). In this review, we mainly focus on our current understanding of the interplay between the immune system and tumors that process through pyroptosis, and debate their use as potential therapeutic targets.
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http://dx.doi.org/10.1111/cas.15059DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8486185PMC
October 2021

Caspase-8: A key protein of cross-talk signal way in "PANoptosis" in cancer.

Int J Cancer 2021 10 29;149(7):1408-1420. Epub 2021 May 29.

Department of Gastrointestinal Oncology, Harbin Medical University Cancer Hospital, Harbin, Heilongjiang, China.

Cysteinyl aspartate specific proteinase (Caspase)-8 has long been considered a promoter of apoptosis and part of the mechanism by which cytotoxic drugs kill cancer cells. With the continuous exploration of the types of programmed cell death, an increasing number of studies have confirmed that caspase-8 plays an important role in cancer. Recently, scholars have proposed the term "PANoptosis," which mainly includes three programmed cell death modes, namely pyroptosis, apoptosis and necroptosis. In addition to mediating endogenous apoptotic pathways, caspase-8 can also participate in the cleavage of gasdermin (GSDM) family proteins to induce pyroptosis. Furthermore, the expression of enzymatically inactive caspase-8 (C362S) can cause embryonic lethality and inflammatory tissue destruction in mice by inducing necroptosis and pyroptosis. Therefore, the activation and deletion of caspase-8 enzyme activity, as well as the knockout of the coding gene, are closely related to "PANoptosis." In addition, caspase-8 can also improve the tumor microenvironment and enhance tumor antiimmunity. Studies have shown that caspase-8 is also associated with tumor growth and invasion, angiogenesis and metastasis, therapeutic resistance and poor clinical outcomes. Therefore, it is very important to measure the cancer-promoting and anticancer effects of caspase-8 and find a balance, and to study its role in the effect of "PANoptosis" in depth. This article reviews the role of caspase-8 in "PANoptosis" in cancer to provide new strategies and targets for cancer.
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http://dx.doi.org/10.1002/ijc.33698DOI Listing
October 2021

The caspase-3/GSDME signal pathway as a switch between apoptosis and pyroptosis in cancer.

Cell Death Discov 2020 28;6:112. Epub 2020 Oct 28.

Department of Gastrointestinal Oncology, Harbin Medical University Cancer Hospital, 150 Haping St, Nangang District, Harbin, Heilongjiang 150081 P. R. China.

Apoptosis has long been recognized as a mechanism that kills the cancer cells by cytotoxic drugs. In recent years, studies have proved that pyroptosis can also shrink tumors and inhibit cells proliferation. Both apoptosis and pyroptosis are caspase-dependent programmed cell death pathways. Cysteinyl aspartate specific proteinase-3 (Caspase-3) is a common key protein in the apoptosis and pyroptosis pathways, and when activated, the expression level of tumor suppressor gene Gasdermin E (GSDME) determines the mechanism of tumor cell death. When GSDME is highly expressed, the active caspase-3 cuts it and releases the N-terminal domain to punch holes in the cell membrane, resulting in cell swelling, rupture, and death. When the expression of GSDME is low, it will lead to the classical mechanism of tumor cell death, which is apoptosis. More interestingly, researchers have found that GSDME can also be located upstream of caspase-3, connecting extrinsic, and intrinsic apoptotic pathways. Then, promoting caspase-3 activation, and forming a self-amplifying feed-forward loop. GSDME-mediated pyroptosis is correlated with the side effects of chemotherapy and anti-tumor immunity. This article mainly reviews the caspase-3/GSDME signal pathway as a switch between apoptosis and pyroptosis in cancer, to provide new strategies and targets for cancer treatment.
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http://dx.doi.org/10.1038/s41420-020-00349-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7595122PMC
October 2020

The influence of photodynamic therapy on the Warburg effect in esophageal cancer cells.

Lasers Med Sci 2020 Oct 8;35(8):1741-1750. Epub 2020 Feb 8.

Department of Gastrointestinal Oncology, Harbin Medical University Cancer Hospital, Harbin, 150081, Heilongjiang, China.

To investigate whether the Warburg effect is a key modulator on the resistance mechanism of photodynamic therapy (PDT). Glycolysis was examined by the test of lactate product and glucose uptake at different post-PDT time points. Cell viability was detected by the CCK-8 assay and cell proliferation was detected by colony formation assay. The expression of glycolysis and related proteins were examined by western blotting. Target gene was silenced by RNAi. In the present study, we assessed the effect of PDT on cancer cell glycolysis. Our team has demonstrated that pyruvate kinase M2 (PKM2), a key speed-limiting enzyme of glycolysis, was significantly overexpressed in patients with esophageal cancer. Our results in the present study showed that PKM2 was downregulated, and lactate product and glucose uptake were inhibited in cells exposed to 5-aminolevulinic acid (5-ALA)-mediated PDT at 4 h after treatment. However, at 24 h after PDT, we observed a substantial increase in PKM2 expression, lactate product, and glucose uptake. Moreover, silencing of PKM2 gene abrogated the upregulatory effect of PDT on glycolysis at late post-PDT period. 2-Deoxy-D-glucose (2-DG) is a recognized chemical inhibitor of glycolysis. The combined treatment of 2-DG and PDT significantly inhibited tumor growth in vitro at 24 h. These results demonstrate that PDT drives the Warburg effect in a time-dependent manner, and PKM2 plays an important role in this progress, which indicated that PKM2 may be a potential molecular target to increase the sensitivity of esophageal cancer cells to PDT.
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http://dx.doi.org/10.1007/s10103-020-02966-8DOI Listing
October 2020

Development of a loop-mediated isothermal amplification coupled lateral flow dipstick targeting erm(41) for detection of Mycobacterium abscessus and Mycobacterium massiliense.

AMB Express 2019 Jan 23;9(1):11. Epub 2019 Jan 23.

National Tuberculosis Reference Laboratory, Chinese Center for Disease Control and Prevention, Changping, Changbai Road 155, Beijing, 102206, China.

Mycobacterium abscessus (M. abscessus) and Mycobacterium massiliense (M. massiliense) are major pathogens that cause post-surgical wound infection and chronic pulmonary disease. Although they are closely related subspecies of M. abscessus complex, their infections are associated with different drug-resistance and cure rate. In the present study, a loop-mediated isothermal amplification (LAMP) coupled with lateral flow dipstick (LFD) method was developed to simultaneous detect M. abscessus and M. massiliense, via specific erm(41) gene. The amplification was carried out at 65 °C for only 60 min, and the results could be visualized on a lateral flow strip. Positive results only occurred in M. abscessus and M. massiliense, no cross-reaction with other mycobacterial species was observed. Therefore, the cost-effective MABC (M. abscessus complex)-LAMP-LFD method developed here was able to correct the diagnose of M. abscessus and M. massiliense infection in a short time. Thus, this method could be used to guide clinicians in treatment of M. abscessus group infections.
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http://dx.doi.org/10.1186/s13568-019-0734-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6344564PMC
January 2019

ALA/LA ameliorates glucose toxicity on HK-2 cells by attenuating oxidative stress and apoptosis through the ROS/p38/TGF-β pathway.

Lipids Health Dis 2017 Nov 16;16(1):216. Epub 2017 Nov 16.

School of Public Health, Southeast University, Nanjing, 210009, China.

Background: Growing evidence indicates that oxidative stress (OS) plays a pivotal role in Diabetic nephropathy (DN). In a previous study we demonstrated that ALA/LA protected HK-2 cells against high glucose-induced cytotoxicity. So we aimed to establish the glucose injury model of HK-2 cells and investigate the beneficial effects of ALA/LA on high glucose-induced excessive production of TGF-β1 and the possible mechanisms mediating the effects.

Methods: The expression of OS markers in high glucose-induced HK-2 cells treated with ALA/LA., including the antioxidant enzymes and reactive oxygen species (ROS) production, as well as the apoptosis rate were assayed by ELISA and flow cytometry. The p38/transforming growth factor β (TGF-β) signal pathway were measured by real-time RT-PCR and western blot.

Results: The modeling condition of glucose toxicity on HK-2 cells was at the glucose concentration of 40.9 mM. ALA/LA can significantly increase the activities of antioxidant enzymes and decrease ROS production stimulated by high glucose. The study also found that ALA/LA caused a decrease in the apoptosis rate and TGF-β level of HK-2 cells under high glucose stress through the ROS/p38 pathway.

Conclusions: ALA/LA exerts protective effects in vitro through inhibition of ROS generation, down regulation of the activation of the p38MAPK pathway and the expression of TGF-β in HK-2 cells.
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http://dx.doi.org/10.1186/s12944-017-0611-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5691398PMC
November 2017

[Study on VNTR diversity of clinical Mycobacterium tuberculosis isolates from Qinghai].

Zhonghua Liu Xing Bing Xue Za Zhi 2015 Oct;36(10):1158-61

Medical School of Qinghai University, Xining 810001, China.

Objective: To investigate the variable number tandem repeats (VNTR) genetic polymorphisms, genotyping and distribution pattern of clinical Mycobacterium (M.) tuberculosis isolates from Qinghai province.

Methods: The clinical M. tuberculosis strains isolated from the patients with tuberculosis and related background data were collected from Qinghai Provincial Center for Disease Control and Prevention from 2009 to 2012. Genotyping was conducted by using multiple locus VNTR analysis (MLVA). Genomic DNA was extracted and 15 VNTR loci were amplified with PCR and the PCR products were detected with gel electrophoresis. The VNTR diversity and clusters of genotyping were analyzed with BioNumerics (Version 5.0).

Results: A total of 251 clinical M. tuberculosis isolates were analyzed with 15 VNTR loci showing that there were great genetic diversity in these isolates. Six of the 15 VNTR loci, showed that the Hunter-Gaston index (HGI) were higher than 0.6, in which the highest resolution was MIRU26. The clusters of genotyping showed that these isolates could be categorized into four gene clusters and 238 genotypes. The four gene clusters accounted for 4.9%, 91.9%, 1.6% and 1.6% of the clinical isolates, respectively.

Conclusion: The results showed that there is great variety of VNTR genetic polymorphisms in clinical M. tuberculosis isolates in Qinghai province.
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October 2015

[Genotyping of Mycobacterium tuberculosis isolates circulating in Qinghai province by spoligotyping].

Zhonghua Yu Fang Yi Xue Za Zhi 2015 Jun;49(6):565-7

Departnrent of public health, medical college of Qinghai university, Xihing 810008, China; Email:

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June 2015

[Effect of grain-bean package, grain-bean package dietary fiber and single whole grain dietary fiber on dyslipidemia rats].

Wei Sheng Yan Jiu 2014 May;43(3):372-7

Objective: To observe and compare the effects of grain-bean package, dietary fiber (DF) extracted from grain-bean package, and DF from grain corn on the blood lipids and fatty acid synthase (FAS) activity in high-fat, high-cholesterol feeding induced dyslipidemia rats, and observe its effects on regulation of sterol regulatory element protein-1c (SREBP-1c) mRNA expression in rat liver.

Methods: Consolidation 50 SD rats of clean grade feeding adaptation for one week, randomly assigned into normal control group, hyperlipidemia model group, grain-bean package group, grain-bean package DF group and grain corn group. Feed with corresponding diets for 8 weeks, and measure the total cholesterol (TC), triglyceridaemia (TG), high density lipoprotein cholesterol (HDL-C), fasting blood glucose (FBG), FAS, SREBP-1c mRNA of all groups.

Result: Compared with control group, TC, TG, FBG levels of hyperlipidemia model group were significantly increased (P < 0.05). Compared with model group, TC, TG, FBG levels of grain-bean package group, grain-bean package DF group were significantly decreased, HDL-C levels significantly increased, and activity of FAS, regulation of SREBP-1c were significantly decreased (P < 0.05).

Conclusion: The Grain-bean package dietary fiber can improve blood lipids levels of dyslipidemia rats, and decrease FAS activity and SREBP-1c mRNA expression.
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May 2014

[Research of the whole grain-soybean compound package to regulate the cholesterol metabolism by SREBP-2, LDLR and visfatin].

Wei Sheng Yan Jiu 2013 Mar;42(2):196-202

Department of Nutrition and Food Hygiene, School of Public Health, Southeast University, Nanjing 210009, China.

Objective: To discuss the possible mechanism of the whole grain-soybean compound package on dyslipidaemia rats.

Methods: 44 SD rats were randomly divided into four groups: the hyperlipidaemic group, the rice-flour group, the whole grain-soybean compound package group and the negative control group by lipid profiles, fed with corresponding feed for eight weeks. Serum total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C) were measured before and after the test. At the end of the experiment, blood samples were taken from the femoral artery, the rat organs were collected and weighted. Serum Visfatin levels and SREBP-2 mRNA and LDLR mRNA in the liver were measured.

Results: Compared with the hyperlipidaemic group and rice-flour group, the body weight, serum TC, TG and LDL-C of whole grain-soybean compound package group were significantly decreased (P < 0.05), HDL-C was significantly increased (P < 0.05). Visfatin levels were significantly decreased (P < 0.05). Gene expressions of SREBP-2 and LDLR were significantly increased (P < 0.05). Gene expression of Visfatin in whole grain-soybean compound package group was significantly lower than that in hyperlipidaemia group and rice-flour group (P < 0.05).

Conclusion: Whole grain-soybean compound package can improve the serum lipid profiles and Visfatin of rats fed with a high fat diet. The possible mechanism is that the whole grain-soybean compound package can activate the expression of SREBP-2, LDLR and Visfatin. And then enhance the expressing activity of regulate the cholesterol metabolism by SREBP-2, LDLR and Visfatin. Ultimately, to reduce the level of rats' cholesterol, and then ameliorate the dyslipidaemia of rats.
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March 2013

[Effect of compound whole grain-soybean on oxidative stress].

Wei Sheng Yan Jiu 2013 Jan;42(1):1-5, 9

School of Public Health, Southeast University, Nanjing 210009, China.

Objective: To observe the in vitro oxidation resistance of compound whole grain and the effect on improving the disorder of lipid metabolism and the oxidative stress in rats.

Methods: Make extracting of compound whole grain, rice, flour and black rice, method use chemical colorimetry to detect total antioxidant capacity, hydroxyl radical (*OH) and superoxide anion (O2-*). Forty-four male SD rats were divided into four groups in random: negative control group, model control group, white rice-flour group and compound whole grain. All 4 groups were fed for 8 weeks with different experimental diets. Weight, total cholesterol (TC), triglyceride (TG ), high density lipoprotein cholesterol (HDL-C) were detected in serum. Malondialdehyde (MDA), total antioxidant capacity (T-AOC), super oxygen dehydrogenizes (SOD), glutathione peroxidase (GSH-Px) were detected in serum and liver.

Results: The T-AOC, the ability of body cleaning hydroxyl radical and superoxide anion were enhanced,quite with the black rice. In all 3 treatment groups, compound whole grain group had higher HDL-C, T-AOC, SOD, GSH-Px, while TC, TG, MDA were lower. Compared with negative control groups, there is no significant difference.

Conclusion: Compound whole grain can have good effect on oxidative stress. This effect is the important mechanism of lipid metabolism disorders.
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January 2013

[Protective effect of ALA on high glucose induced cellular injury of LLC-PK1 cell].

Wei Sheng Yan Jiu 2012 Mar;41(2):191-4

School of Public Health, Southeast University, Nanjing 210009, China.

Objective: Made LLC-PK1 damage model induced by high glucose and observing the protection effect and its mechanisms of LLC-PK1 injury induced by high glucose.

Methods: The proliferation of LLC-PK1 induced by high glucose was tested by CCK-8 and the apoptosis rat and the contents of reactive oxygen species (ROS) of LLC-PK1 damaged by high glucose was observed by flow cytometry after administration of different concentration alpha-linolenic acid (ALA).

Result: High glucose could obviously inhibit the proliferation of LLC-PK1 The apoptotic rates of LLC-PK1 intervened by ALA (50-100 micromol/L) in the preconditioning group and the persistent intervention group were lower than those in the positive control group (P < 0.05). The contents of ROS of LLC-PK1 in the persistent intervention group were lower than those in the positive control group when the concentration of ALA were from 10 micromol/L to 100 micromol/L (P < 0.05, P < 0.01). The contents of ROS of LLC-PK1 in the preconditioning group were lower than those in the positive control group when the concentration of ALA was 50 micromol/L (P < 0.05).

Conclusion: The model of LLC-PK1 induced by high glucose provided fine chances for the intervention of renal tubular epithelial cells in DN. ALA were expected to be a protectant to prevent high glucose damage of renal tubulars. Decreasing the active oxygen generation may be one of the mechanism of the protective effects on LLC-PK1 by ALA.
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March 2012
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