Publications by authors named "Mingrong He"

7 Publications

  • Page 1 of 1

Optimized seeding rate and nitrogen topdressing ratio for simultaneous improvement of grain yield and bread-making quality in bread wheat sown on different dates.

J Sci Food Agric 2021 Jun 18. Epub 2021 Jun 18.

National Key Lab. of Crop Biology, Key Lab. of Crop Ecophysiology and Farming System, Ministry of Agriculture, Agronomy College of Shandong Agricultural University, Tai'an, P.R. China.

Background: Sowing date, seeding rate, and nitrogen (N) topdressing ratio have strong effects on grain yield (GY) and bread-making quality (BQ) in bread wheat. Simultaneous improvement in GY and BQ in bread wheat has long been a challenge due to the inverse relationship between GY and grain protein concentration (GPC). In this study, we investigated whether the GY and BQ of bread wheat sown on different dates could be improved simultaneously by optimizing the seeding rate and the N topdressing ratio.

Results: Delaying sowing beyond a certain period led to decreases in both GY and BQ. Optimizing the seeding rate and N topdressing ratio enhanced the N uptake during pre- and post-anthesis, as well as N remobilization during grain filling for all wheat plants sown on different dates, thereby increasing the GPC and the total N per grain (N ). Consequently, grain protein composition was improved, resulting in an increased glutenin/gliadin ratio, sodium dodecyl sulfate-insoluble glutenin/total glutenin (i.e., glutenin polymerization index), and high-molecular-weight glutenin subunit/ low-molecular-weight glutenin subunit (HMW-GS/LMW-GS) ratio. Increased GPC and improved grain protein composition enhanced BQ.

Conclusion: The mechanism underlying simultaneous improvement in GY and GPC as well as N was the greater increase in N accumulation in grains per unit area relative to increases in GY, or total grain number per unit area. The GY and BQ can be improved simultaneously regardless of sowing date by optimizing the seeding rate and N topdressing ratio via enhanced N uptake and N remobilization into grains. © 2021 Society of Chemical Industry.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/jsfa.11366DOI Listing
June 2021

Proteomic analysis of wheat seeds produced under different nitrogen levels before and after germination.

Food Chem 2021 Mar 25;340:127937. Epub 2020 Aug 25.

State Key Laboratory of Crop Biology, Agronomy College, Shandong Agricultural University, Tai'an, Shandong Province 271018, PR China. Electronic address:

The objective of this study was to investigate differentially abundant proteins (DAPs) of wheat seeds produced under two nitrogen levels (0 and 240 kg/ha) before and after germination. We selected samples at 8 and 72 h after imbibition (HAI) to identify DAPs by iTRAQ. The results showed 190 and 124 DAPs at 8 and 72 HAI, respectively. Alpha-gliadin and chlorophyll a-b binding protein showed the biggest difference in abundance before and after germination. In GO enrichment analysis, the most significantly enriched GO term was nutrient reservoir activity at 8 HAI and endopeptidase inhibitor activity at 72 HAI. Moreover, many DAPs involved in mobilization of stored nutrients and photosynthesis were mapped to KEGG pathways. Dough development time, dough stability time and seedling chlorophyll content under N240 were significantly higher than those under N0, which validated the results of proteomic analysis. These results are crucial for food nutrition and food processing.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.foodchem.2020.127937DOI Listing
March 2021

Effects of Nitrogen Level during Seed Production on Wheat Seed Vigor and Seedling Establishment at the Transcriptome Level.

Int J Mol Sci 2018 Oct 31;19(11). Epub 2018 Oct 31.

State Key Laboratory of Crop Biology, Agronomy College, Shandong Agricultural University, Tai'an 271018, China.

Nitrogen fertilizer is a critical determinant of grain yield and seed quality in wheat. However, the mechanism of nitrogen level during seed production affecting wheat seed vigor and seedling establishment at the transcriptome level remains unknown. Here, we report that wheat seeds produced under different nitrogen levels (N0, N168, N240, and N300) showed significant differences in seed vigor and seedling establishment. In grain yield and seed vigor, N0 and N240 treatments showed the minimum and maximum, respectively. Subsequently, we used RNA-seq to analyze the transcriptomes of seeds and seedlings under N0 and N240 at the early stage of seedling establishment. Gene Ontology (GO) term enrichment analysis revealed that dioxygenase-activity-related genes were dramatically upregulated in faster growing seedlings. Among these genes, the top three involved linoleate 9S-lipoxygenase (, , and ). Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis revealed that pathways involved in nutrient mobilization and the antioxidant system showed enhanced expression under N240. Moreover, seeds with faster growing seedlings had a higher gene expression level of α-amylase, which was consistent with α-amylase activity. Taken together, we propose a model for seedling establishment and seed vigor in response to nitrogen level during seed production.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ijms19113417DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6274887PMC
October 2018

Pursuing sustainable productivity with millions of smallholder farmers.

Nature 2018 03 7;555(7696):363-366. Epub 2018 Mar 7.

Institute of Agricultural Resources and Environment, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, China.

Sustainably feeding a growing population is a grand challenge, and one that is particularly difficult in regions that are dominated by smallholder farming. Despite local successes, mobilizing vast smallholder communities with science- and evidence-based management practices to simultaneously address production and pollution problems has been infeasible. Here we report the outcome of concerted efforts in engaging millions of Chinese smallholder farmers to adopt enhanced management practices for greater yield and environmental performance. First, we conducted field trials across China's major agroecological zones to develop locally applicable recommendations using a comprehensive decision-support program. Engaging farmers to adopt those recommendations involved the collaboration of a core network of 1,152 researchers with numerous extension agents and agribusiness personnel. From 2005 to 2015, about 20.9 million farmers in 452 counties adopted enhanced management practices in fields with a total of 37.7 million cumulative hectares over the years. Average yields (maize, rice and wheat) increased by 10.8-11.5%, generating a net grain output of 33 million tonnes (Mt). At the same time, application of nitrogen decreased by 14.7-18.1%, saving 1.2 Mt of nitrogen fertilizers. The increased grain output and decreased nitrogen fertilizer use were equivalent to US$12.2 billion. Estimated reactive nitrogen losses averaged 4.5-4.7 kg nitrogen per Megagram (Mg) with the intervention compared to 6.0-6.4 kg nitrogen per Mg without. Greenhouse gas emissions were 328 kg, 812 kg and 434 kg CO equivalent per Mg of maize, rice and wheat produced, respectively, compared to 422 kg, 941 kg and 549 kg CO equivalent per Mg without the intervention. On the basis of a large-scale survey (8.6 million farmer participants) and scenario analyses, we further demonstrate the potential impacts of implementing the enhanced management practices on China's food security and sustainability outlook.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/nature25785DOI Listing
March 2018

A loose endosperm structure of wheat seed produced under low nitrogen level promotes early germination by accelerating water uptake.

Sci Rep 2017 06 8;7(1):3116. Epub 2017 Jun 8.

State Key Laboratory of Crop Biology, Agronomy College, Shandong Agricultural University, Tai'an, Shandong Province, 271018, P.R. China.

Water uptake is the fundamental requirement for the initiation and completion of seed germination that is a vital phase in the life cycle of seed plants. We found that seeds produced under four nitrogen levels showed significantly different germination speed. The objective of this study was to study the mechanism of rapid seed germination and explore which pathways and genes play critical roles in radicle protrusion. Anatomical data revealed that seed protein content affected endosperm structure of seeds. Moreover, scanning electron microscope maps showed that faster germinated seeds had a looser endosperm structure compared with other seeds. Subsequently, high throughout RNA-seq data were used to compare the transcriptomes of imbibed seeds with different germination speed. Gene ontology (GO) term enrichment analysis revealed that cell wall metabolism related genes significantly up-regulated in faster germinated seeds. In these genes, the top four were chitinase that had about fourfold higher expression in faster germinated seeds. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that faster germinated seeds had enhanced expression in glutathione metabolism. By combining these results, we propose a model for nitrogen fertilizer affects germination speed of wheat seed, which provide new insights into seed germination.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-017-03333-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5465190PMC
June 2017

Producing more grain with lower environmental costs.

Nature 2014 Oct 3;514(7523):486-9. Epub 2014 Sep 3.

College of Resources &Environmental Sciences, China Agricultural University, Beijing 100193, China.

Agriculture faces great challenges to ensure global food security by increasing yields while reducing environmental costs. Here we address this challenge by conducting a total of 153 site-year field experiments covering the main agro-ecological areas for rice, wheat and maize production in China. A set of integrated soil-crop system management practices based on a modern understanding of crop ecophysiology and soil biogeochemistry increases average yields for rice, wheat and maize from 7.2 million grams per hectare (Mg ha(-1)), 7.2 Mg ha(-1) and 10.5 Mg ha(-1) to 8.5 Mg ha(-1), 8.9 Mg ha(-1) and 14.2 Mg ha(-1), respectively, without any increase in nitrogen fertilizer. Model simulation and life-cycle assessment show that reactive nitrogen losses and greenhouse gas emissions are reduced substantially by integrated soil-crop system management. If farmers in China could achieve average grain yields equivalent to 80% of this treatment by 2030, over the same planting area as in 2012, total production of rice, wheat and maize in China would be more than enough to meet the demand for direct human consumption and a substantially increased demand for animal feed, while decreasing the environmental costs of intensive agriculture.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/nature13609DOI Listing
October 2014

[Effects of weak light and high temperature stress after anthesis on flag leaf chlorophyll fluorescence and grain fill of wheat].

Ying Yong Sheng Tai Xue Bao 2005 Nov;16(11):2117-21

Key Laboratory of Wheat Cultivation Physiology and Genetic Improvement of Agriculture Ministry, College of Agronomy, Shandong Agricultural University, Taian, China.

A field experiment with wheat showed that after anthesis, the photosystem II (PS II), photochemical efficiency (Fv/Fm) and photosynthetic rate (Pn) of flag leaf decreased substantially, its actual quantum yield of PS II electron transport (phiPS II) and photochemical quenching co-efficient (qP) declined slightly, while its non-photochemical quenching (NPQ) increased slightly three days after shading, compared with the control. Three days after high temperature treatment, the Fv/Fm, Pn, phiPS II and qP decreased significantly, while the NPQ increased slightly. During the course of recovery after the stresses, the parameters fluorescence and Pn which were stressed by shading and high temperature at prophase recovered slightly, while those stressed at metaphase decreased all along, indicating that the damage at prophase was reversible, but the stress at metaphase accelerated the caducity of flag leaves. The grain-filling processes of different treatments were modeled with Logistic equation, which showed that the decline of per kernel weight was mainly caused by the decrease of mean filling rate, maximal filling rate, and slow-increase period filling rate. Filling duration, occurrence time of maximal filling rate, duration of slight-increase period, and slight-increase period filling rate were less affected by weak light and high temperature.
View Article and Find Full Text PDF

Download full-text PDF

Source
November 2005
-->