Publications by authors named "Ming-Yu Zhao"

12 Publications

  • Page 1 of 1

Enhanced weathering as a trigger for the rise of atmospheric O level from the late Ediacaran to the early Cambrian.

Sci Rep 2019 07 23;9(1):10630. Epub 2019 Jul 23.

CAS Key Laboratory of Crust-Mantle Materials and Environments, School of Earth and Space Sciences, University of Science and Technology of China, Hefei, 230026, China.

A shift toward a higher oxygen level in both ocean and atmosphere systems during the late Ediacaran to the early Cambrian has been suggested from multiple indirect proxies. However, the mechanism and magnitude of this oxidation remain unclear. To solve this issue, we measured carbon isotopes in both carbonate and organic matter as well as their trace element compositions for an Ediacaran-Cambrian sequence in the Lower Yangtze basin, South China. The δC and δC excursions of this sequence are coupled and can be compared with contemporaneous global carbon isotope curves. A 2‰ rise in ΔC occurred from the late Ediacaran to the early Cambrian, suggesting a substantial increase in atmospheric oxygen level from 16% to 30% of the present atmospheric level (PAL). Furthermore, the distribution pattern of rare earth elements and the concentrations of water-insoluble elements in the carbonates indicate a sudden enhancement in chemical weathering of the continental crust during the early Cambrian, which may be a trigger for the rise of atmospheric O level. Both the supply of a large amount of nutrients due to the enhanced continental weathering and the contemporary increase of atmospheric oxygen concentrations may have promoted the appearance of large metazoans in the early Cambrian.
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http://dx.doi.org/10.1038/s41598-019-47142-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6650434PMC
July 2019

Structural variation of transition metal-organic frameworks using deep eutectic solvents with different hydrogen bond donors.

Dalton Trans 2019 Jul 13;48(27):10199-10209. Epub 2019 Jun 13.

Key Laboratory of Flexible Electronics (KLOFE) & Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University (NanjingTech), 30 South Puzhu Road, Nanjing 211816, P.R. China.

Deep eutectic solvents (DESs) have attracted extensive attention in the field of material synthesis as green solvents. They have similar physical and chemical properties to the traditional ionic liquids (ILs) while being much cheaper and more environmentally friendly. Herein, seven transition metal-organic frameworks, namely [NH][Zn(BTC)(NH)]·HO (1), [Cu(PDC)(NH)] (2), [Co(HBTC)(e-urea)]·(e-urea)·1/4HO (3), K(NH)[Mn(PZDC)] (4), [NH][Mn(BTC)(HO)] (5), [CHNH][Mn(HBTC)(BTC)·3HO (6), and [Co(BTC)(urea)]·2HO (7), were synthesized in deep eutectic solvents of choline chloride and urea/e-urea/m-urea (HBTC = 1,3,5-benzenetricarboxylic acid; HPDC = 2,6-pyridinedicarboxylic acid; HPZDC = 3,5-pyrazoledicarboxylic acid; e-urea = ethylene urea; m-urea = N,N-dimethylurea). Of particular interest is the fact that the utilization of different hydrogen bond donors in DES mixtures can lead to the formation of different frameworks. The multiple roles of hydrogen bond donors in the reactions were discussed. Furthermore, compound 7 exhibited catalytic activity for the oxidation of styrene, and thus it can be used as a heterogeneous catalyst due to its good stability. These results promote the understanding of the application of DESs in synthesizing novel transition metal-organic frameworks.
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http://dx.doi.org/10.1039/c9dt01050eDOI Listing
July 2019

Tracking the rise of eukaryotes to ecological dominance with zinc isotopes.

Geobiology 2018 07 5;16(4):341-352. Epub 2018 Jun 5.

Geology and Geophysics, Yale University, New Haven, Connecticut.

The biogeochemical cycling of zinc (Zn) is intimately coupled with organic carbon in the ocean. Based on an extensive new sedimentary Zn isotope record across Earth's history, we provide evidence for a fundamental shift in the marine Zn cycle ~800 million years ago. We discuss a wide range of potential drivers for this transition and propose that, within available constraints, a restructuring of marine ecosystems is the most parsimonious explanation for this shift. Using a global isotope mass balance approach, we show that a change in the organic Zn/C ratio is required to account for observed Zn isotope trends through time. Given the higher affinity of eukaryotes for Zn relative to prokaryotes, we suggest that a shift toward a more eukaryote-rich ecosystem could have provided a means of more efficiently sequestering organic-derived Zn. Despite the much earlier appearance of eukaryotes in the microfossil record (~1700 to 1600 million years ago), our data suggest a delayed rise to ecological prominence during the Neoproterozoic, consistent with the currently accepted organic biomarker records.
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http://dx.doi.org/10.1111/gbi.12289DOI Listing
July 2018

Circulating tumor DNA detection in lung cancer patients before and after surgery.

Sci Rep 2016 09 19;6:33519. Epub 2016 Sep 19.

Department of Thoracic Surgery, The General Hospital of People's Liberation Army, Beijing, China.

Circulating tumor DNA (ctDNA) in peripheral blood is a "liquid biopsy" that contains representative tumor information including gene mutations. Additionally, repeated ctDNA samples can be easily obtained to monitor response to treatment and disease progression, which may be especially valuable to lung cancer patients with tumors that cannot be easily biopsied or removed. To investigate the changes in ctDNA after surgical tumor resection, tumor and blood samples obtained before and after surgery were collected prospectively from 41 non-small lung cancer (NSCLC) patients. Somatic driver mutations in tumor DNA (tDNA) and pre- and post-op plasma ctDNA sample pairs were identified by targeted sequencing in several genes including EGFR, KRAS, and TP53 with an overall study concordance of 78.1% and sensitivity and specificity of 69.2% and 93.3%, respectively. Importantly, the frequency of 91.7% of ctDNA mutations decreased after surgery and these changes were observed as little as 2 days post-op. Moreover, the presence of ctDNA had a higher positive predictive value than that of six tumor biomarkers in current clinical use. This study demonstrates the use of targeted sequencing to reliably identify ctDNA changes in response to treatment, indicating a potential utility of this approach in the clinical management of NSCLC.
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http://dx.doi.org/10.1038/srep33519DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5027588PMC
September 2016

Circulating Tumor DNA Detection in Early-Stage Non-Small Cell Lung Cancer Patients by Targeted Sequencing.

Sci Rep 2016 08 24;6:31985. Epub 2016 Aug 24.

Department of Thoracic Surgery, Peking University People's Hospital, Beijing, China.

Circulating tumor DNA (ctDNA) isolated from peripheral blood has recently been shown to be an alternative source to detect gene mutations in primary tumors; however, most previous studies have focused on advanced stage cancers, and few have evaluated ctDNA detection in early-stage lung cancer. In the present study, blood and tumor samples were collected prospectively from 58 early-stage non-small lung cancer (NSCLC) patients (stages IA, IB, and IIA) and a targeted sequencing approach was used to detect somatic driver mutations in matched tumor DNA (tDNA) and plasma ctDNA. We identified frequent driver mutations in plasma ctDNA and tDNA in EGFR, KRAS, PIK3CA, and TP53, and less frequent mutations in other genes, with an overall study concordance of 50.4% and sensitivity and specificity of 53.8% and 47.3%, respectively. Cell-free (cfDNA) concentrations were found to be significantly associated with some clinical features, including tumor stage and subtype. Importantly, the presence of cfDNA had a higher positive predictive value than that of currently used protein tumor biomarkers. This study demonstrates the feasibility of identifying plasma ctDNA mutations in the earliest stage lung cancer patients via targeted sequencing, demonstrating a potential utility of targeted sequencing of ctDNA in the clinical management of NSCLC.
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http://dx.doi.org/10.1038/srep31985DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4995492PMC
August 2016

Seeking a geochemical identifier for authigenic carbonate.

Nat Commun 2016 Mar 7;7:10885. Epub 2016 Mar 7.

CAS Key Laboratory of Crust-Mantle Materials and Environments, School of Earth and Space Sciences, University of Science and Technology of China, Hefei 230026, China.

Authigenic carbonate was recently invoked as a third major global carbon sink in addition to primary marine carbonate and organic carbon. Distinguishing the two carbonate sinks is fundamental to our understanding of Earth's carbon cycle and its role in regulating the evolution of atmospheric oxygen. Here, using microscale geochemical measurements of carbonates in Early Triassic strata, we show that the growth of authigenic carbonate follows a different trajectory from primary marine carbonate in a cross-plot of uranium concentration and carbon isotope composition. Thus, a combination of the two geochemical variables is able to distinguish between the two carbonate sinks. The temporal distribution of authigenic carbonates in the Early Triassic strata suggests that the increase in the extent of carbonate authigenesis acted as a negative feedback to the elevated atmospheric CO2 concentration.
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http://dx.doi.org/10.1038/ncomms10885DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4786675PMC
March 2016

Circulating tumor DNA identified by targeted sequencing in advanced-stage non-small cell lung cancer patients.

Cancer Lett 2016 Jan 12;370(2):324-31. Epub 2015 Nov 12.

Department of Lung Cancer Surgery, Tianjin Medical University General Hospital, Tianjin, China. Electronic address:

Non-small cell lung cancers (NSCLC) have unique mutation patterns, and some of these mutations may be used to predict prognosis or guide patient treatment. Mutation profiling before and during treatment often requires repeated tumor biopsies, which is not always possible. Recently, cell-free, circulating tumor DNA (ctDNA) isolated from blood plasma has been shown to contain genetic mutations representative of those found in the primary tumor tissue DNA (tDNA), and these samples can readily be obtained using non-invasive techniques. However, there are still no standardized methods to identify mutations in ctDNA. In the current study, we used a targeted sequencing approach with a semi-conductor based next-generation sequencing (NGS) platform to identify gene mutations in matched tDNA and ctDNA samples from 42 advanced-stage NSCLC patients from China. We identified driver mutations in matched tDNA and ctDNA in EGFR, KRAS, PIK3CA, and TP53, with an overall concordance of 76%. In conclusion, targeted sequencing of plasma ctDNA may be a feasible option for clinical monitoring of NSCLC in the near future.
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http://dx.doi.org/10.1016/j.canlet.2015.11.005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7495502PMC
January 2016

Molecular cloning and characterization of interferon regulatory factor 9 (IRF9) in Japanese flounder, Paralichthys olivaceus.

Fish Shellfish Immunol 2014 Aug 14;39(2):138-44. Epub 2014 May 14.

Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao 266003, China; College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China.

Interferon regulatory factor 9 (IRF9) in mammals is known to be involved in antiviral response. In this study, we studied the structure, mRNA tissue distribution and regulation of IRF9 from Japanese flounder, Paralichthys olivaceus. The cDNA sequence of IRF9 is 3305 bp long, containing an open reading frame (ORF) of 1308 bp that encodes a peptide of 435 amino acids. The predicted protein sequence shares 33.7-72.0% identity to other fish IRF9s. Japanese flounder IRF9 possesses a DNA-binding domain (DBD), an IRF association domain (IAD), two nuclear localization signals (NLSs) and a proline-rich domain (PRD). The IRF9 transcripts were detectable in all examined tissues of healthy Japanese flounders, with higher levels in the head kidney, kidney, liver and spleen. The IRF9 mRNA levels were up-regulated in the gills, head kidney, spleen and muscle when challenged with polyinosinic:polycytidylic acid (poly I:C) or lymphocystis disease virus (LCDV). The up-regulations were stronger and arose earlier in the case of poly I:C treatment in most tested organs in a 7-day time course, with maximum increases ranging from 1.37- to 8.59-fold and peak time points from 3 h to 3 d post injection depending on different organs, relative to those in the case of LCDV treatment which ranged from 1.32- to 3.21-fold and from 18 h to 3 d post injection, respectively. The highest and earliest inductions were detected in the spleen in both challenge cases, while the inductions by LCDV in the muscle were quite faint. These results demonstrate a role of Japanese flounder IRF9 in the host's antiviral responses.
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http://dx.doi.org/10.1016/j.fsi.2014.05.002DOI Listing
August 2014

The transcription factor AtDOF4.2 regulates shoot branching and seed coat formation in Arabidopsis.

Biochem J 2013 Jan;449(2):373-88

State Key Lab of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.

Plant-specific DOF (DNA-binding with one finger)-type transcription factors regulate various biological processes. In the present study we characterized a silique-abundant gene AtDOF (Arabidopsis thaliana DOF) 4.2 for its functions in Arabidopsis. AtDOF4.2 is localized in the nuclear region and has transcriptional activation activity in both yeast and plant protoplast assays. The T-M-D motif in AtDOF4.2 is essential for its activation. AtDOF4.2-overexpressing plants exhibit an increased branching phenotype and mutation of the T-M-D motif in AtDOF4.2 significantly reduces branching in transgenic plants. AtDOF4.2 may achieve this function through the up-regulation of three branching-related genes, AtSTM (A. thaliana SHOOT MERISTEMLESS), AtTFL1 (A. thaliana TERMINAL FLOWER1) and AtCYP83B1 (A. thaliana CYTOCHROME P450 83B1). The seeds of an AtDOF4.2-overexpressing plant show a collapse-like morphology in the epidermal cells of the seed coat. The mucilage contents and the concentration and composition of mucilage monosaccharides are significantly changed in the seed coat of transgenic plants. AtDOF4.2 may exert its effects on the seed epidermis through the direct binding and activation of the cell wall loosening-related gene AtEXPA9 (A. thaliana EXPANSIN-A9). The dof4.2 mutant did not exhibit changes in branching or its seed coat; however, the silique length and seed yield were increased. AtDOF4.4, which is a close homologue of AtDOF4.2, also promotes shoot branching and affects silique size and seed yield. Manipulation of these genes should have a practical use in the improvement of agronomic traits in important crops.
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http://dx.doi.org/10.1042/BJ20110060DOI Listing
January 2013

NIMA-related kinase NEK6 affects plant growth and stress response in Arabidopsis.

Plant J 2011 Dec 14;68(5):830-43. Epub 2011 Sep 14.

State Key Lab of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.

The NIMA-related kinases (NEKs) are a family of serine/threonine kinases involved largely in cell cycle control in fungi, mammals and other eukaryotes. In Arabidopsis, NEK6 is involved in the regulation of epidermal cell morphogenesis. However, other roles of NEK6 in plants are less well understood. Here we report functions of NEK6 in plant growth, development and stress responses in Arabidopsis. NEK6 transcripts and proteins are induced by ethylene precursor ACC and salt stress. Expression of other NEK genes except NEK5 is also responsive to the two treatments. Overexpression and mutant analysis disclose that the NEK6 gene increases rosette growth, seed yield and lateral root formation. However, NEK6 appears to play a negative role in the control of seed size. The gene also promotes plant tolerance to salt stress and osmotic stress in its overexpressing plants. The NEK6 gene may achieve its function through suppression of ethylene biosynthesis and activation of CYCB1;1 and CYCA3;1 expression. Our present study reveals new functions of the NEK6 gene in plant growth and stress tolerance, and manipulation of NEK6 may improve important agronomic traits in crop plants.
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http://dx.doi.org/10.1111/j.1365-313X.2011.04733.xDOI Listing
December 2011

[Treatment of child ultra-radius distal fracture of back to back with manipulative reduction and external fixation of splint and plaster].

Zhongguo Gu Shang 2009 Aug;22(8):635-6

Zhengzhou Orthopaedics Hospitol, Zhengzhou 450052, Henan, China.

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August 2009

[Effect of Ce3+ on volatile fatty acid concentrations during anaerobic granular sludge digestion].

Huan Jing Ke Xue 2009 Apr;30(4):1115-9

State Key Laboratory of Pollution Control and Resource Reuse, School of Environment, Nanjing University, Nanjing 210093, China.

Batch experiments were conducted to investigate the effect of Ce3+ on volatile fatty acid(VFA) concentrations by anaerobic granular sludge digestion using D-Glucose and acetic sodium as substrate in the state of stabilization and restart-up. Results show that when the concentration of Ce3+ is lower than 1 mg/L, VFA concentration decreases, which suggests the transformation of butyric acid to acetic acid and acetic acid to methane is promoted. When the concentration of Ce3+ is 1-10 mg/L, the bacterial activity decreases and decomposition of the acetic acid and butyric acid becomes more difficult compared with the control. Adding Ce3+ brings little change in the constitution of VFA: 96% of VFA is acetic acid and butyric acid, while the propionic acid accounts for less than 3%. With the acetic sodium as the sole carbon and energy source, adding 0.05 mg/L Ce3+ could accelerate acetate degradation. After being conserved for 4 months, the activity of the Ce-containing anaerobic granular sludge is higher than that of the Ce-free sludge. The present of Ce contributes to the restart-up of anaerobic reactors.
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April 2009