Publications by authors named "Ming Zheng"

543 Publications

Mitophagy-regulated mitochondrial health strongly protects the heart against cardiac dysfunction after acute myocardial infarction.

J Cell Mol Med 2022 Jan 18. Epub 2022 Jan 18.

Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Peking University Health Science Center, and Key Laboratory of Molecular Cardiovascular Science, Ministry of Education, Beijing, China.

Autophagy including mitophagy serves as an important regulatory mechanism in the heart to maintain the cellular homeostasis and to protect against heart damages caused by myocardial infarction (MI). The current study aims to dissect roles of general autophagy and specific mitophagy in regulating cardiac function after MI. By using Beclin1 , Fundc1 knockout (KO) and Fundc1 transgenic (TG) mouse models, combined with starvation and MI models, we found that Fundc1 KO caused more severe mitochondrial and cardiac dysfunction damages than Beclin1 after MI. Interestingly, Beclin1 caused notable decrease of total autophagy without detectable change to mitophagy, and Fundc1 KO markedly suppressed mitophagy but did not change the total autophagy activity. In contrast, starvation increased total autophagy without changing mitophagy while Fundc1 TG elevated total autophagy and mitophagy in mouse hearts. As a result, Fundc1 TG provided much stronger protective effects than starvation after MI. Moreover, Beclin1 /Fundc1 TG showed increased total autophagy and mitophagy to a level comparable to Fundc1 TG per se, and completely reversed Beclin1 -caused aggravation of mitochondrial and cardiac injury after MI. Our results reveal that mitophagy but not general autophagy contributes predominantly to the cardiac protective effect through regulating mitochondrial function.
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http://dx.doi.org/10.1111/jcmm.17190DOI Listing
January 2022

Decomplexation of Cu(II)-EDTA by synergistic activation of persulfate with alkali and CuO: Kinetics and activation mechanism.

Sci Total Environ 2022 Jan 7;817:152793. Epub 2022 Jan 7.

Department of Civil and Environmental Engineering, University of Alberta, Edmonton, Alberta T6G 1H9, Canada.

Heavy metals usually coexist with a variety of chelating agents to form heavy metal complexes in industrial wastewater. The decomplexation of heavy metal complexes is the crucial step before the removal of heavy metals via alkaline precipitation process. An efficient synergistic activation of persulfate (PS) with alkali and CuO was used for the simultaneous decomplexation of Cu-ethylenediamine tetraacetic acid (Cu(II)-EDTA) (3.14 mM) and the Cu(II) precipitation. The experimental results demonstrated that nearly complete removal of Cu(II) could be achieved by synergistic activation of PS with alkali and CuO at pH 11 after 2 h of decomplexation reaction. However, sole PS could not effectively decomplex Cu(II)-EDTA (13.5%), while the alkaline activation of PS could accomplish 57.0% removal of Cu(II). Radical scavenger tests indicated that reactive oxygen species (ROS) including SO, •OH and O were responsible for the decomplexation of Cu(II)-EDTA in the synergistic activation of PS with alkali and CuO. As a heterogeneous activator, CuO possessed excellent reusability and long-lasting catalytic activity and the rate constant value (k) of Cu(II) removal showed an increase (from 0.0326 min in the first cycle to 0.0491 min in the 24 cycle) with 24 cycles experiments. Furthermore, the biotoxicity evaluation of treated solution revealed that the biotoxicity of Cu(II)-EDTA contaminated wastewater could be effectively mitigated by the synergistic activation of PS with alkali and CuO because of the efficient precipitation of Cu(II) and oxidative degradation of EDTA organic ligands, which was favorable for the subsequent biochemical treatment.
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http://dx.doi.org/10.1016/j.scitotenv.2021.152793DOI Listing
January 2022

Single Nucleotide Polymorphisms of IL-33 Gene Correlated with Renal Allograft Fibrosis in Kidney Transplant Recipients.

J Immunol Res 2021 13;2021:8029180. Epub 2021 Dec 13.

Department of Urology, The Second Affiliated Hospital with Nanjing Medical University, Nanjing 210011, China.

Background: Nowadays, renal allograft survival is confined by the development of allograft fibrosis. Previous studies have reported interleukin-33 (IL-33) upregulated significantly in patients with chronic renal allograft dysfunction, and it could induce renal tubular epithelial to mesenchymal transition (EMT), which eventually contributed to renal allograft fibrosis. Our study intended to detect the underlying association between single nucleotide polymorphisms (SNPs) of IL-33 gene and renal allograft fibrosis in kidney transplant recipients.

Methods: We collected blood samples from 200 renal transplant recipients for the identification of SNPs and transplanted kidney tissue samples for identifying differentially expressed genes (DEGs). Intersection of SNP-related genes and DEGs was conducted for further analysis. Relationships between these SNPs and renal allograft fibrosis were evaluated by the inheritance models. Immunohistochemical (IHC) staining and western blotting (WB) were used to detect the expression of IL-33 and the markers of EMT in human kidney tissues obtained from control and chronic renal allograft dysfunction (CAD) patients. In vitro, we detected the progressions of EMT-related markers and the levels of MAPK signaling pathway mediators after transfecting IL-33 mutant plasmids in HK2 cells.

Results: Three intersected genes including IL-33 genes were significantly expressed. IL-33 expression was validated in kidney tissues by IHC and WB. Thirty-nine IL-33-related SNPs were identified in targeted sequencing, in which 26 tagger SNPs were found by linkage disequilibrium analysis for further analysis. General linear models indicated sirolimus administration significantly influenced renal allograft fibrosis ( < 0.05), adjustment of which was conducted in the following analysis. By multiple inheritance model analyses, SNP rs10975519 of IL-33 gene was found closely related to renal allograft fibrosis ( < 0.005). Furthermore, HK2 cells transfected with mutated plasmid of rs10975519 showed stronger mobility and migration ability. Moreover, IL-33 mutant plasmids could promote the IL-33-induced EMT through the sustained activation of p38 MAPK signaling pathway in HK2 cells.

Conclusion: In our study, rs10975519 on the IL-33 gene was found to be statistically associated with the development of renal allograft fibrosis in kidney transplant recipients. This process may be related to the IL-33-induced EMT and sustained activation of p38 MAPK signaling pathway.
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http://dx.doi.org/10.1155/2021/8029180DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8689233PMC
December 2021

Research Note: Comparison on laying behavior and clutch traits among Zhedong white geese (Anser cygnoides), Sichuan white geese (Anser cygnoides), and Hungarian geese (Anser anser).

Poult Sci 2021 Dec 3;101(2):101594. Epub 2021 Dec 3.

Key Laboratory of Animal Genetics and Breeding and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China. Electronic address:

Egg laying is an important reproductive behavior of female poultry. Here, we investigated the laying behavior and clutch traits of different goose breeds, specifically, Zhedong white geese (Anser cygnoides), Sichuan white geese (Anser cygnoides) and Hungarian geese (Anser anser). A total of 108 geese in their first laying year were selected. Their laying rhythm was monitored daily by video cameras during the egg-laying period. The results showed that the prenatal behavior included nest hunting, nest site selection and nesting, as well as laying behavior included prenatal crouching, anal contraction until oviposition and postnatal resting. There were some differences in laying times among the 3 breeds. Approximately 70% of the eggs were laid between 03:00 and 12:00 in Zhedong white geese and Sichuan white geese, while the Hungarian geese laid throughout the day. Also, the clutch length and the oviposition interval differed among these 3 breeds. Their average clutch lengths were approximately 14.07, 12.14 and 19.15 days in Zhedong white geese, Sichuan white geese, and Hungarian geese, respectively. While their oviposition intervals showed 2 peaks (39 and 47 h), 3 peaks (41, 50 and 53 h), and 1 peak (46 h). Moreover, there were significant correlation between egg yield and clutch length in Zhedong white geese (r = 0.951), Sichuan white geese (r = 0.987) and Hungarian geese (r = 0.991), respectively. Taken together, the difference in laying behavior among the breeds was mainly reflected in laying time and oviposition intervals, Zhedong white geese and Sichuan white geese have egg laying time preference and short oviposition intervals, which was helpful not only to understand egg-laying process, but also to manage egg-laying geese scientifically and improve egg production.
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http://dx.doi.org/10.1016/j.psj.2021.101594DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8703062PMC
December 2021

Anti-influenza T cells in bronchoalveolar microenvironment of critically severe COVID-19 patients.

Authors:
Ming Zheng

Crit Care 2021 12 20;25(1):444. Epub 2021 Dec 20.

Institute of Military Cognition and Brain Sciences, Academy of Military Medical Sciences, 27 Taiping Road, Beijing, 100850, China.

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http://dx.doi.org/10.1186/s13054-021-03871-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8687697PMC
December 2021

Oxidative stress in human gingival fibroblasts from periodontitis versus healthy counterparts.

Oral Dis 2021 Dec 14. Epub 2021 Dec 14.

Department of Periodontology, Peking University School and Hospital of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology, Beijing, China.

Objective: Elevated p53 promotes oxidative stress and production of pro-inflammatory cytokines in liposaccharide (LPS)-treated healthy human gingival fibroblasts (HGFs). This study compared oxidative stress, production of inflammatory cytokines, and p53 expression in HGFs from patients with chronic periodontitis (CP) and healthy subjects in vitro upon LPS from Porphyromonas gingivalis challenge.

Methods: Human gingival fibroblasts were isolated from 6 biopsies-3 from healthy donors and 3 from diseased area in CP (Grade B, Stage III). HGFs were cultured with or without 1 μg/ml 24 h LPS. Oxidative stress was assessed by analyzing the level of reactive oxygen species (ROS). Mitochondrial membrane potential and respiration were determined by immunofluorescence and respirometry, respectively. Tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-1β were determined by enzyme-linked immunosorbent assay. P53 expression was monitored by Western blot and immunofluorescence.

Results: Human gingival fibroblasts from CP exhibited increased levels of mitochondrial p53, enhanced ROS production, decreased mitochondrial membrane potential, increased mitochondrial oxygen consumption, and increased secretion of TNF-α, IL-6, and IL-1β, as compared to HGFs from healthy donors. Moreover, LPS exacerbated these changes.

Conclusion: Human gingival fibroblasts from CP exhibited stronger basal and LPS-inducible oxidative stress and inflammatory response as compared to HGFs from healthy subjects by increased p53 in mitochondria.
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http://dx.doi.org/10.1111/odi.14103DOI Listing
December 2021

Using Artificial Neural Network Condensation to Facilitate Adaptation of Machine Learning in Medical Settings by Reducing Computational Burden: Model Design and Evaluation Study.

JMIR Form Res 2021 Dec 8;5(12):e20767. Epub 2021 Dec 8.

Department of Nuclear Science and Engineering, Massachusetts Institute of Technology, Cambridge, MA, United States.

Background: Machine learning applications in the health care domain can have a great impact on people's lives. At the same time, medical data is usually big, requiring a significant number of computational resources. Although this might not be a problem for the wide adoption of machine learning tools in high-income countries, the availability of computational resources can be limited in low-income countries and on mobile devices. This can limit many people from benefiting from the advancement in machine learning applications in the field of health care.

Objective: In this study, we explore three methods to increase the computational efficiency and reduce model sizes of either recurrent neural networks (RNNs) or feedforward deep neural networks (DNNs) without compromising their accuracy.

Methods: We used inpatient mortality prediction as our case analysis upon review of an intensive care unit dataset. We reduced the size of RNN and DNN by applying pruning of "unused" neurons. Additionally, we modified the RNN structure by adding a hidden layer to the RNN cell but reducing the total number of recurrent layers to accomplish a reduction of the total parameters used in the network. Finally, we implemented quantization on DNN by forcing the weights to be 8 bits instead of 32 bits.

Results: We found that all methods increased implementation efficiency, including training speed, memory size, and inference speed, without reducing the accuracy of mortality prediction.

Conclusions: Our findings suggest that neural network condensation allows for the implementation of sophisticated neural network algorithms on devices with lower computational resources.
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http://dx.doi.org/10.2196/20767DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8701705PMC
December 2021

Rapid detection of HS gas driven by the catalysis of flower-like α-BiMoO and its visual performance: A combined experimental and theoretical study.

J Hazard Mater 2022 Feb 20;424(Pt D):127734. Epub 2021 Nov 20.

Key Laboratory of Functional Inorganic Material Chemistry, Ministry of Education, School of Chemistry and Materials Science, Heilongjiang University, Harbin 150080, China. Electronic address:

Metal oxide semiconductor (MOSs) are attractive materials for the development of HS gas sensors. However, detecting HS with short response and recovery times while also lowering the limit of detection to sub-ppb levels remains a significant challenge. We therefore developed flower-like α-BiMoO microspheres for HS gas detection that provide fast response and recovery times (3 and 22 s, respectively, for 100 ppm HS), while also reducing the limit of detection to 1 ppb. The sensor performs well in terms of sensitivity, reproducibility, long-term stability, including humidity stability. X-ray photoelectron spectroscopy (XPS) and density functional theory (DFT) calculations revealed that HS dissociates readily on the reduced α-BiMoO surface and that Mo plays a catalytic role, accelerating the rate of HS decomposition and enabling a fast response. Moreover, test strips containing α-BiMoO were also prepared, which enabled the naked eye detection of ppm-level HS gas at room temperature; a light-yellow to orange to brown color change occurs when exposed to HS, due to its conversion into stable sulfides. This work expands the application of α-BiMoO to HS gas sensing, and provides a strategy for the use of MOSs as sensor materials for the detection of other gases.
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http://dx.doi.org/10.1016/j.jhazmat.2021.127734DOI Listing
February 2022

Is the bond strength of zirconia-reinforced lithium silicate lower than that of lithium disilicate? A systematic review and metaanalysis.

J Prosthodont Res 2021 Dec 1. Epub 2021 Dec 1.

Fujian Key Laboratory of Oral Diseases & Fujian Provincial Engineering Research Center of Oral Biomaterial & Stomatological Key Laboratory of Fujian College and University, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, China.

Purpose: This study systematically reviewed the literature to compare the bond strength of resin composites with that of zirconia-reinforced lithium silicate (ZLS) and lithium disilicate (LD).

Study Selection: This review was structured based on the Preferred Reporting Items for Systematic Reviews and MetaAnalyses (PRISMA 2020) statement. This study was registered at the International Prospective Register of Systematic Reviews (CRD42021256900). Studies were searched via PubMed, Web of Science, and Google Scholar databases without language or publication year limits. In vitro studies that evaluated the bond strength of the resin composites to ZLS and LD were included. The risk of bias in all the included articles was evaluated. Statistical analyses were performed using the Review Manager software (version 5.3, Cochrane Collaboration, Oxford, UK).

Results: Of the 90 potentially related articles, the full texts of 16 articles were evaluated after screening. Finally, sevenstudies were included in the qualitative synthesis and meta-analysis. All the studies presented a medium risk of bias. The results showed no significant difference in bond strength between the ZLS and LD groups (P = 0.94, mean difference=0.08, and 95% confidence interval=-1.93 to 2.10). However, a significant difference was found in the subgroup analysis considering different types of aging treatments (P = 0.0008) and different types of bond strength tests (P < 0.00001).

Conclusion: The bond strength of resin composites was found to be similar to that of ZLS and LD, but different aging treatments and bond strength tests exhibited varying effects on the bond strength.
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http://dx.doi.org/10.2186/jpr.JPR_D_20_00112DOI Listing
December 2021

Optical Detection of Stereoselective Interactions with DNA-Wrapped Single-Wall Carbon Nanotubes.

J Am Chem Soc 2021 Dec 29;143(49):20628-20632. Epub 2021 Nov 29.

Materials Science and Engineering Division, National Institute of Standards and Technology, 100 Bureau Drive, Gaithersburg, Maryland 20899, United States.

DNA-wrapped carbon nanotubes have been explored increasingly as sensitive near-infrared fluorescence probes for biomolecules. However, notably missing in previous studies is an inquiry on stereoselective interactions between DNA-wrapped carbon nanotubes and biomolecules. Here, enantiopure (+) and (-)(6,5), and (-)(8,3) as well as achiral (11,0) carbon nanotubes wrapped with specific resolving DNA sequences are used to demonstrate their stereoselective detection of amino acid enantiomers. Furthermore, stereoselective sensing abilities are found to be retained by dispersions containing a multitude of chiral nanotube structures. The fluorescence response profiles of six different DNA-wrapped carbon nanotube dispersions to nine standard amino acids, and their enantiomers, demonstrate that DNA-wrapped carbon nanotubes are exquisitely sensitive to the stereoconfiguration and side-chain functionality of amino acids in a manner that is dependent on both DNA sequence and nanotube chirality. Implications of our findings are discussed in the context of developing a machine learning-aided multiplexed biosensing scheme called a molecular perceptron.
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http://dx.doi.org/10.1021/jacs.1c11372DOI Listing
December 2021

Abnormal mitochondrial structure and function are retained in gingival tissues and human gingival fibroblasts from patients with chronic periodontitis.

J Periodontal Res 2022 Jan 26;57(1):94-103. Epub 2021 Nov 26.

Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing, China.

Background And Objective: The abnormal structure and function of mitochondria in cells is closely associated with inflammatory diseases. However, the physiology of mitochondria within gingival tissues and human gingival fibroblasts (HGFs) in patients with chronic periodontitis (CP) remains unclear. The objective of this study was to investigate the structure profile and function of mitochondria in gingival tissues and in HGFs derived from patients with or without CP. These features of mitochondria in HGFs were further analyzed when HGFs were induced by lipopolysaccharide (LPS) from Porphyromonas gingivalis (P.g).

Methods: Gingival tissues and HGFs were collected from CP and healthy patients. Mitochondrial structure was assessed by transmission electron microscopy. Tissues or cells lysis was performed for mitochondrial DNA (mtDNA) quantification, and real-time polymerase chain reaction (RT-PCR) tests were used to determine mtDNA copy numbers. Western blot analysis was used to evaluate autophagy-related protein (ATG)-5, microtubule-associated protein light chain 3 (LC3), and mitochondrial matrix protein pyruvate dehydrogenase kinase isozyme 2 (PDK2) levels in tissues and HGFs from CP and healthy individuals.

Results: Tissues and HGFs from CP showed a significant greater mitochondrial structure destruction, lower mtDNA level, increased ATG5, LC3-II, and lower PDK2 protein levels than those of healthy individuals. In addition, LPS from P.g also triggered the same results in HGFs from healthy donors. Moreover, the challenge of HGFs from CP with LPS worsened these parameters.

Conclusion: Mitochondrial structure and function within gingival tissues and HGFs from CP individuals were abnormal compared to those from healthy donors, and LPS could promote mitochondrial destruction.
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http://dx.doi.org/10.1111/jre.12941DOI Listing
January 2022

COVID-19-Related Rumor Content, Transmission, and Clarification Strategies in China: Descriptive Study.

J Med Internet Res 2021 12 23;23(12):e27339. Epub 2021 Dec 23.

Department of Epidemiology and Health Statistics, Hunan Provincial Key Laboratory of Clinical Epidemiology, Xiangya School of Public Health, Central South University, Changsha, China.

Background: Given the permeation of social media throughout society, rumors spread faster than ever before, which significantly complicates government responses to public health emergencies such as the COVID-19 pandemic.

Objective: We aimed to examine the characteristics and propagation of rumors during the early months of the COVID-19 pandemic in China and evaluated the effectiveness of health authorities' release of correction announcements.

Methods: We retrieved rumors widely circulating on social media in China during the early stages of the COVID-19 pandemic and assessed the effectiveness of official government clarifications and popular science articles refuting those rumors.

Results: We show that the number of rumors related to the COVID-19 pandemic fluctuated widely in China between December 1, 2019 and April 15, 2020. Rumors mainly occurred in 3 provinces: Hubei, Zhejiang, and Guangxi. Personal social media accounts constituted the major source of media reports of the 4 most widely distributed rumors (the novel coronavirus can be prevented with "Shuanghuanglian": 7648/10,664, 71.7%; the novel coronavirus is the SARS coronavirus: 14,696/15,902, 92.4%; medical supplies intended for assisting Hubei were detained by the local government: 3911/3943, 99.2%; asymptomatically infected persons were regarded as diagnosed COVID-19 patients with symptoms in official counts: 322/323, 99.7%). The number of rumors circulating was positively associated with the severity of the COVID-19 epidemic (ρ=0.88, 95% CI 0.81-0.93). The release of correction articles was associated with a substantial decrease in the proportion of rumor reports compared to accurate reports. The proportions of negative sentiments appearing among comments by citizens in response to media articles disseminating rumors and disseminating correct information differ insignificantly (both correct reports: χ=0.315, P=.58; both rumors: χ=0.025, P=.88; first rumor and last correct report: χ=1.287, P=.26; first correct report and last rumor: χ=0.033, P=.86).

Conclusions: Our results highlight the importance and urgency of monitoring and correcting false or misleading reports on websites and personal social media accounts. The circulation of rumors can influence public health, and government bodies should establish guidelines to monitor and mitigate the negative impact of such rumors.
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http://dx.doi.org/10.2196/27339DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8709421PMC
December 2021

Assessing the effectiveness of an app-based child unintentional injury prevention intervention for caregivers of rural Chinese preschoolers: protocol for a cluster randomized controlled trial.

BMC Public Health 2021 11 20;21(1):2137. Epub 2021 Nov 20.

Department of Epidemiology and Health Statistics, Hunan Provincial Key Laboratory of Clinical Epidemiology, Xiangya School of Public Health, Central South University, Changsha, 410078, China.

Background: Compared to urban children, children living in rural areas of most countries, including China, are at higher risk of suffering unintentional injuries. Most proven injury prevention interventions, however, are rarely implemented in rural China due to lack of resources. Mobile health interventions are low-cost and easy-to-implement, facilitating implementing injury prevention in resource-limited areas (e.g., rural areas). This study is designed and implemented to examine the effectiveness of an app-based intervention for unintentional injury prevention among rural preschoolers in China.

Methods: A single-blind, 18-month, parallel-group cluster randomized controlled trial with 1:1 allocation ratio will be implemented in 2 rural areas of China (Yang County, Shaanxi Province, and Shicheng County, Jiangxi Province). In total, at least 3508 rural caregivers of preschoolers aged 3-6 years old who own a smartphone will be recruited from 24 preschools. Clusters will be randomized at the preschool level and allocated to the control group (receiving routine school-based education plus app-based parenting education excluding unintentional injury prevention) or the intervention group (receiving routine school-based education plus app-based parenting education including unintentional injury prevention). External support strategies will be adopted by local partners to minimize user fatigue, non-compliance, and attrition. Data collection will be conducted at baseline and then every 3 months during the 18-month follow-up time period. Intention-to-treat data analysis will be implemented. Missing values will be imputed by using the Expectation Maximization algorithm. Generalized estimating equation will test the overall effectiveness of the app-based intervention. A per-protocol sensitivity analysis will be conducted to test the robustness of results. Subgroup analyses will follow the strategies for primary analyses. The primary outcome measure is the incidence rate of unintentional injury among preschoolers during the study period. Secondary outcome measures comprise longitudinal changes in caregiver's attitudes, caregiver-reported supervision behaviors, and caregiver-assessed home environment safety surrounding child unintentional injury prevention in the last week using a standardized audit instrument.

Discussion: The app-based intervention is expected to be feasible and effective over the 18-month intervention period. If the app is demonstrated effective as hypothesized, we will initiate processes to generalize and popularize it broadly to rural child caregivers across China.

Trial Registration: ChiCTR2000037606 , registered on August 29, 2020.
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http://dx.doi.org/10.1186/s12889-021-12156-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8606071PMC
November 2021

Dye-polyoxometalate coordination polymer as a photo-driven electron pump for photocatalytic radical coupling reactions.

Chem Commun (Camb) 2021 Nov 30;57(95):12812-12815. Epub 2021 Nov 30.

State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, P. R. China.

To alleviate diffusion-limited photoinduced electron transfer (PET) in solution, a triphenylamine-derived dye and a Keggin polyoxometalate-type electron relay were coupled into a coordination polymer to photoinduce long-lived charge-separation pairs with enough reductive/oxidative potential to pump multiple electrons unidirectionally from external electron donors to acceptors, thus furnishing photocatalytic radical couplings to afford value-added α-amino C-H arylation products.
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http://dx.doi.org/10.1039/d1cc04209bDOI Listing
November 2021

The Gradient of Immune/Inflammatory Response and COVID-19 Prognosis with Therapeutic Implications.

Authors:
Ming Zheng

Front Immunol 2021 29;12:739482. Epub 2021 Oct 29.

Institute of Military Cognition and Brain Sciences, Academy of Military Medical Sciences, Beijing, China.

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http://dx.doi.org/10.3389/fimmu.2021.739482DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8586492PMC
November 2021

Tissue resident memory T cells in the respiratory tract.

Mucosal Immunol 2021 Oct 20. Epub 2021 Oct 20.

Department of Microbiology and Immunology, The University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Melbourne, VIC, 3000, Australia.

Owing to their capacity to rapidly spread across the population, airborne pathogens represent a significant risk to global health. Indeed, several of the past major global pandemics have been instigated by respiratory pathogens. A greater understanding of the immune cells tasked with protecting the airways from infection will allow for the development of strategies that curb the spread and impact of these airborne diseases. A specific subset of memory T-cell resident in both the upper and lower respiratory tract, termed tissue-resident memory (Trm), have been shown to play an instrumental role in local immune responses against a wide breadth of both viral and bacterial infections. In this review, we discuss factors that influence respiratory tract Trm development, longevity, and immune surveillance and explore vaccination regimes that harness these cells, such approaches represent exciting new strategies that may be utilized to tackle the next global pandemic.
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http://dx.doi.org/10.1038/s41385-021-00461-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8526531PMC
October 2021

Fully-automated SPE coupled to UHPLC-MS/MS method for multiresidue analysis of 26 trace antibiotics in environmental waters: SPE optimization and method validation.

Environ Sci Pollut Res Int 2021 Oct 16. Epub 2021 Oct 16.

Key Laboratory of Organic Compound Pollution Control Engineering, School of Environmental and Chemical Engineering, Shanghai University, Shanghai, 200444, China.

Achieving simultaneous determination of antibiotic multiresidues in environmental waters by solid phase extraction (SPE) coupled with ultra-high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) with detection limits ≤ ng L is still a huge challenge. Moreover, the offline SPE procedure was performed manually, costly, and time-consuming, while the online SPE required precision pretreatment instruments that require highly-skilled personnel. In this paper, a fully automated SPE coupled with UHPLC-MS/MS method was developed for analysis of antibiotics (sulfonamides, quinolones, and macrolides) in water matrices. Sample preparation optimization included SPE materials and configuration (HLB disks), sample volume (500-1000 mL), and pH (pH = 3) with a flow rate at 2~5 mL min, and an elution procedure with 2 × 6 mL methanol, and 2 × 6 mL acetone. Meanwhile, the parameters for UHPLC-MS/S detection of analytes were optimized, including LC retention time, and MS parameters. The instrumental limits of detection (LOD) and quantification (LOQ) of analytes ranged from 0.01-0.72 μg L and 0.05-2.39 μg L, respectively, with satisfactory linear calibration (R > 0.995) and precision (< 9.9%). Recoveries in spike samples ranged between 77.5-104.9% in pure water, 59.4-97.8% in surface water (SW), and 58.2-108.6% in wastewater effluent (WWE) with relative standard deviations ≤ 12.8%. The matrix effects observed for most analytes were suppression (0-28.1%) except for five analytes having presented enhancement (0-14.6 %) in SW or WWE. This method can basically meet the needs of trace antibiotic residues detection in waters, with examples of concentrations of detected antibiotics being lower than LOQ (LLQ) -94.47 ng L in WWEs and LLQ-15.47 ng L in SW in the lower reaches of the Yangtze River Basin.
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http://dx.doi.org/10.1007/s11356-021-15947-3DOI Listing
October 2021

Circular RNA hsa_circ_0007507 May Serve as a Biomarker for the Diagnosis and Prognosis of Gastric Cancer.

Front Oncol 2021 14;11:699625. Epub 2021 Sep 14.

Department of Laboratory Medicine, Affiliated Hospital of Nantong University, Nantong, China.

Purpose: The morbidity and mortality of gastric cancer (GC) remain high worldwide. In recent years, circular RNAs (circRNAs) have attracted widespread attention among cancer researchers due to the stable ring structure. The present work aims to find serum circRNA biomarkers that can be used in clinical applications and effective diagnosis.

Methods: Hsa_circ_0007507 was extracted through circRNA sequencing. Exonuclease digestion assay, actinomycin D, agarose gel electrophoresis (AGE), and Sanger sequencing verified the potential of hsa_circ_0007507 as a biomarker. Besides, a real-time fluorescent quantitative polymerase chain reaction (RT-qPCR) was established to detect the level of expression of hsa_circ_0007507. Twenty cases of GC and the paired adjacent tissues were collected to verify its overexpression. Then, serum samples from 30 cases of colorectal cancer, 30 cases of thyroid cancer, and 30 cases of breast cancer were collected to verify their organ specificity. Additionally, serum samples from 80 healthy people, 62 gastritis patients, 31 intestinal metaplasia patients, and 100 GC patients were collected, and the diagnostic efficacy was evaluated through analysis of the receiver operating characteristic (ROC) curve. Furthermore, 16 post-operative GC samples, samples of 65 relapsed patients and 36 non-relapsed patients were collected to evaluate the prognosis of GC.

Results: The level of expression of hsa_circ_0007507 in GC tissues was up-regulated ( = 0.0121), which was consistent with the results of circRNA sequencing. Exonuclease digestion assay and actinomycin D confirmed that hsa_circ_0007507 had a stable structure and a longer half-life. In the analysis of organ specificity experiments, serum hsa_circ_0007507 did not have specificity for patients with colorectal cancer ( = 0.5319), thyroid cancer ( = 0.5422), or breast cancer ( = 0.5178). Analysis of diagnostic efficacy indicated that the expression of hsa_circ_0007507 was significantly higher than that of normal people (0.0001); the area under the ROC (AUC) was 0.832 (95% CI: 0.771-0.892); the diagnostic power of hsa_circ_0007507 was higher than that of CEA (AUC = 0.765, 95% CI: 0.697-0.833) and CA199 (AUC = 0.587, 95% CI: 0.504-0.67). Through diagnosis using a combination of the three, GC patients could be distinguished from normal people (AUC = 0.849), and higher diagnostic efficiency could be achieved. The expression of serum hsa_circ_0007507 in GC patients significantly decreased after surgery ( = 0.001). Besides, the expression of serum hsa_circ_0007507 in patients with post-operative recurrence was significantly up-regulated again ( = 0.0139).

Conclusions: Serum hsa_circ_0007507 is differentially expressed in GC patients, post-operative GC patients, gastritis patients, intestinal metaplasia patients and relapsed patients, suggesting that serum hsa_circ_0007507 can be used as a new diagnostic and dynamic monitoring biomarker for GC.
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http://dx.doi.org/10.3389/fonc.2021.699625DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8477006PMC
September 2021

Carbonylative coupling of simple alkanes and alkenes enabled by organic photoredox catalysis.

Chem Commun (Camb) 2021 Oct 5;57(79):10210-10213. Epub 2021 Oct 5.

College of Chemical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.

A visible-light-driven direct carbonylative coupling of simple alkanes and alkenes the combination of a hydrogen atom transfer process and photoredox catalysis has been demonstrated. Employing the -alkoxyazinium salt as the oxidant and the precursor of an oxygen radical, a variety of α,β-unsaturated ketones could be obtained in a metal-free fashion.
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http://dx.doi.org/10.1039/d1cc04138jDOI Listing
October 2021

Polymorphisms in the gene significantly affect the pharmacokinetics of sirolimus after kidney transplantation.

Pharmacogenomics 2021 09 15;22(14):903-912. Epub 2021 Sep 15.

Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, PR China.

Sirolimus (SIR) is an immunosuppressant with limitations, including a narrow treatment window, multiple adverse reactions and large differences within and among individuals. The correlation between numerous SNPs and SIR in terms of trough concentration in the early stage after kidney transplantation was analyzed. A retrospective cohort study involving 69 kidney transplantation recipients was designed. Blood samples were collected to extract total DNAs, and trough SIR concentrations were measured. Logistic regression was used to analyze the association between SNPs and SIR trough concentrations. At 7 days, 1 month and 3 months, the mean SIR trough concentration of patients in the rs4646453-CC group was significantly higher than that in the rs4646453-AA and rs4646453-CA groups (p < 0.001) and rs15524-AA group was significantly higher than that in the rs15524-AG and rs15524-GG groups (p < 0.001). Our study indicated that both rs4646453 and rs15524 had a certain influence on SIR trough concentration at 7 days, 1 month and 3 months.
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http://dx.doi.org/10.2217/pgs-2021-0083DOI Listing
September 2021

CRISPR/Cas9-targeted mutagenesis of the BnaA03.BP gene confers semi-dwarf and compact architecture to rapeseed (Brassica napus L.).

Plant Biotechnol J 2021 Dec 20;19(12):2383-2385. Epub 2021 Sep 20.

Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Wuhan, China.

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http://dx.doi.org/10.1111/pbi.13703DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8633515PMC
December 2021

Elucidating the Role of Serum tRF-31-U5YKFN8DYDZDD as a Novel Diagnostic Biomarker in Gastric Cancer (GC).

Front Oncol 2021 23;11:723753. Epub 2021 Aug 23.

Medical School of Nantong University, Nantong, China.

Background: Gastric cancer (GC) is one of the malignant tumors with the highest morbidity and mortality in the world. Early diagnosis combined with surgical treatment can significantly improve the prognosis of patients. Therefore, it is urgent to seek higher sensitivity and specificity biomarkers in GC. tRNA-derived small RNAs are a new non-coding small RNA that widely exists in tumor cells and body fluids. In this study, we explore the expression and biological significance of tRNA-derived small RNAs in GC.

Materials And Methods: First of all, we screened the differentially expressed tRNA-derived small RNAs in tumor tissues by high-throughput sequencing. Agarose gel electrophoresis (AGE), Sanger sequencing, and Nuclear and Cytoplasmic RNA Separation Assay were used to screen tRF-31-U5YKFN8DYDZDD as a potential tumor biomarker for the diagnosis of GC. Then, we detected the different expressions of tRF-31-U5YKFN8DYDZDD in 24 pairs of GC and paracancerous tissues, the serum of 111 GC patients at first diagnosis, 89 normal subjects, 48 superficial gastritis patients, and 28 postoperative GC patients by quantitative real-time PCR (qRT-PCR). Finally, we used the receiver operating characteristic (ROC) curve to analyze its diagnostic efficacy.

Results: The expression of tRF-31-U5YKFN8DYDZDD has good stability and easy detection. tRF-31-U5YKFN8DYDZDD was highly expressed in tumor tissue, serum, and cell lines of GC, and the expression was significantly related to TNM stage, depth of tumor invasion, lymph node metastasis, and vascular invasion. The expression of serum tRF-31-U5YKFN8DYDZDD in the GC patients decreased after the operation ( = 0.0003). Combined with ROC curve analysis, tRF-31-U5YKFN8DYDZDD has better detection efficiency than conventional markers.

Conclusions: The expressions of tRF-31-U5YKFN8DYDZDD in the tumor and paracancerous tissues, the serum of GC patients and healthy people, and the serum of GC patients before and after operation were different. tRF-31-U5YKFN8DYDZDD is not only a diagnostic biomarker of GC but also a predictor of poor prognosis.
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http://dx.doi.org/10.3389/fonc.2021.723753DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8419412PMC
August 2021

Genomic insights into the origin, domestication and diversification of Brassica juncea.

Nat Genet 2021 09 6;53(9):1392-1402. Epub 2021 Sep 6.

College of Agronomy, Hunan Agricultural University, Changsha, China.

Despite early domestication around 3000 BC, the evolutionary history of the ancient allotetraploid species Brassica juncea (L.) Czern & Coss remains uncertain. Here, we report a chromosome-scale de novo assembly of a yellow-seeded B. juncea genome by integrating long-read and short-read sequencing, optical mapping and Hi-C technologies. Nuclear and organelle phylogenies of 480 accessions worldwide supported that B. juncea is most likely a single origin in West Asia, 8,000-14,000 years ago, via natural interspecific hybridization. Subsequently, new crop types evolved through spontaneous gene mutations and introgressions along three independent routes of eastward expansion. Selective sweeps, genome-wide trait associations and tissue-specific RNA-sequencing analysis shed light on the domestication history of flowering time and seed weight, and on human selection for morphological diversification in this versatile species. Our data provide a comprehensive insight into the origin and domestication and a foundation for genomics-based breeding of B. juncea.
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http://dx.doi.org/10.1038/s41588-021-00922-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8423626PMC
September 2021

Programmed cell death 5 improves skeletal muscle insulin resistance by inhibiting IRS-1 ubiquitination through stabilization of MDM2.

Life Sci 2021 Nov 1;285:119918. Epub 2021 Sep 1.

Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, P.R. China. Electronic address:

Aims: Insulin resistance is defined as the decreased sensitivity of tissues and organs to insulin and it is the main pathological basis of metabolic syndrome. PDCD5 is widely expressed in tissues including skeletal muscle and liver, but its exact function and the role in insulin resistance has not been studied. The present study is to explore the effect of PDCD5 on insulin resistance in skeletal muscle, the largest target organ of insulin, and its mechanism.

Materials And Methods: Mice were fed with high-fat diet to establish obesity model. C2C12 myoblasts differentiated into myotubes and then were treated with palmitate to induce insulin resistance. Gain-of-function and loss-of-function experiments were performed by infecting C2C12 with adenovirus containing PDCD5 cDNA or PDCD5 shRNA.

Key Findings: PDCD5 protein was first increased and then decreased in the skeletal muscle from high-fat diet induced obese mice and consistently in palmitate induced insulin resistance C2C12 myotubes. Overexpression of PDCD5 in C2C12 cells did not affect the sensitivity to insulin but inhibited the palmitate induced insulin resistance, while knockdown of PDCD5 aggravated the insulin resistance. Mechanistically, PDCD5 interacted with ubiquitin ligase MDM2; overexpression of PDCD5 decreased MDM2 protein level, inhibited the increased interaction of MDM2 with IRS-1 and the degradation of IRS-1 by palmitate stimulation.

Significance: PDCD5 is upregulated during the early stage of insulin resistance in skeletal muscle. The increased PDCD5 inhibits IRS-1 ubiquitination, increases the stability of IRS-1 by interacting with and degrading MDM2, thus providing a protective effect on insulin resistance in skeletal muscle.
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http://dx.doi.org/10.1016/j.lfs.2021.119918DOI Listing
November 2021

Fetoscopy-guided bipolar cord coagulation in selective fetal reduction with complicated monochorionic diamniotic twins: a prospective cohort study.

J Matern Fetal Neonatal Med 2021 Sep 1:1-4. Epub 2021 Sep 1.

Department of Obstetrics and Gynecology, the Affiliated Drum and Tower Hospital of Medical School of Nanjing University, Nanjing, China.

Objective: To assess the feasibility of fetoscopy-guided bipolar umbilical cord coagulation for selective fetal reduction in complicated monochorionic diamniotic (MCDA) twin pregnancies.

Methods: MCDA twins undergoing fetoscopy-guided bipolar cord coagulation (BCC) were enrolled prospectively between December 2015 to March 2020 in a fetal medicine center.

Results: Twenty-three cases undergoing fetoscopy-guided BCC were finally analyzed, including 11 cases for type 2 selective intrauterine growth restriction, 4 cases for twin-twin transfusion syndrome, and 8 cases for a severe discordant anomaly. The overall survival rate was 78.3% (18/23).

Conclusions: Fetoscopy-guided BCC is effective for selective fetal reduction in complicated monochorionic twin pregnancies.
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http://dx.doi.org/10.1080/14767058.2021.1961725DOI Listing
September 2021

CircRNAs and their regulatory roles in cancers.

Mol Med 2021 08 26;27(1):94. Epub 2021 Aug 26.

Department of Laboratory Medicine, Affiliated Hospital of Nantong University, Xisi Road, No.20, Nantong, 226001, Jiangsu, China.

Circular RNAs (circRNAs), a novel type of non-coding RNAs (ncRNAs), have a covalently closed circular structure resulting from pre-mRNA back splicing via spliceosome and ribozymes. They can be classified differently in accordance with different criteria. As circRNAs are abundant, conserved, and stable, they can be used as diagnostic markers in various diseases and targets to develop new therapies. There are various functions of circRNAs, including sponge for miR/proteins, role of scaffolds, templates for translation, and regulators of mRNA translation and stability. Without m7G cap and poly-A tail, circRNAs can still be degraded in several ways, including RNase L, Ago-dependent, and Ago-independent degradation. Increasing evidence indicates that circRNAs can be modified by N-6 methylation (m6A) in many aspects such as biogenesis, nuclear export, translation, and degradation. In addition, they have been proved to play a regulatory role in the progression of various cancers. Recently, methods of detecting circRNAs with high sensitivity and specificity have also been reported. This review presents a detailed overview of circRNAs regarding biogenesis, biomarker, functions, degradation, and dynamic modification as well as their regulatory roles in various cancers. It's particularly summarized in detail in the biogenesis of circRNAs, regulation of circRNAs by m6A modification and mechanisms by which circRNAs affect tumor progression respectively. Moreover, existing circRNA detection methods and their characteristics are also mentioned.
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http://dx.doi.org/10.1186/s10020-021-00359-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8393742PMC
August 2021

Prevalence and risk factors of allergic rhinitis and asthma in the southern edge of the plateau grassland region of northern China: A cross-sectional study.

World Allergy Organ J 2021 Jul 25;14(7):100537. Epub 2021 Jun 25.

Department of Otolaryngology Head and Neck Surgery, Beijing TongRen Hospital, Capital Medical University, Beijing Key Laboratory of Nasal Diseases, Beijing Institute of Otolaryngology, Beijing, China.

Background: The prevalence rates of allergic rhinitis (AR) and asthma in the border region of China may be different from those in the central region of plateau grasslands. A survey was performed to investigate the prevalence and risk factors for AR, asthma, and AR combined with asthma among adults (age ≥ 20 years) residing in the southern border of plateau grasslands in northern China.

Methods: From May to August 2018, a cross-sectional survey was completed by subjects that were selected using a cluster random sampling method. The subjects completed a questionnaire and were administered skin prick tests (SPTs). Risk factors for AR, asthma, and AR combined with asthma were examined by multivariate logistic regression analyses.

Results: A total of 1815 adult subjects in the selected region completed study. The prevalence rates of physician-diagnosed AR, asthma, and AR combined with asthma were 13.9% (253), 9.8% (177), and 2.9% (52), respectively. Among the patients with AR, 20.6% were found to have concurrent asthma; among the patients with asthma, 29.4% were found to have concurrent AR. and pollen were the most common sensitizing pollen types. Approximately 70% of subjects with AR and <30% of asthma patients were sensitized to and pollen. Symptoms of AR and asthma mainly appeared during August. A multivariable logistic regression analysis identified sensitization pollen as an independent risk factor for both AR and AR combined with asthma (AR: OR = 16.23, 95% CI: 10.15-25.96; AR combined with asthma: OR = 6.16, 95% CI: 1.28-29.66). An age >40 years old, family history of asthma, moderate-to-severe AR, adverse food reactions, and mold allergies were independent risk factors for AR combined with asthma.

Conclusions: This study identified the prevalence rates of AR and asthma in the southern borders of the plateau grassland in northern China (>1500 m above sea level). Sensitization pollen is an independent risk factor for AR and AR combined with asthma.
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http://dx.doi.org/10.1016/j.waojou.2021.100537DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8356121PMC
July 2021

Research on endoplasmic reticulum-targeting fluorescent probes and endoplasmic reticulum stress-mediated nanoanticancer strategies: A review.

Colloids Surf B Biointerfaces 2021 Dec 16;208:112046. Epub 2021 Aug 16.

School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang 453007, PR China. Electronic address:

Subcellular localization of organelles can achieve accurate drug delivery and maximize drug efficacy. As the largest organelle in eukaryotic cells, the endoplasmic reticulum (ER) plays an important role in protein synthesis, folding, and posttranslational modification; lipid biosynthesis; and calcium homeostasis. Observing the changes in various metal ions, active substances, and the microenvironment in the ER is crucial for diagnosing and treating many diseases, including cancer. Excessive endoplasmic reticulum stress (ERS) can have a killing effect on malignant cells and can mediate cell apoptosis, proper modulation of ERS can provide new perspectives for the treatment of many diseases, including cancer. Therefore, the ER is used as a new anticancer target in cancer treatment. This review discusses ER-targeting fluorescent probes and ERS-mediated nanoanticancer strategies.
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http://dx.doi.org/10.1016/j.colsurfb.2021.112046DOI Listing
December 2021

Investigating lymphangiogenesis in vitro and in vivo using engineered human lymphatic vessel networks.

Proc Natl Acad Sci U S A 2021 08;118(31)

Department of Biomedical Engineering, Technion - Israel Institute of Technology, Haifa 32000, Israel;

The lymphatic system is involved in various biological processes, including fluid transport from the interstitium into the venous circulation, lipid absorption, and immune cell trafficking. Despite its critical role in homeostasis, lymphangiogenesis (lymphatic vessel formation) is less widely studied than its counterpart, angiogenesis (blood vessel formation). Although the incorporation of lymphatic vasculature in engineered tissues or organoids would enable more precise mimicry of native tissue, few studies have focused on creating engineered tissues containing lymphatic vessels. Here, we populated thick collagen sheets with human lymphatic endothelial cells, combined with supporting cells and blood endothelial cells, and examined lymphangiogenesis within the resulting constructs. Our model required just a few days to develop a functional lymphatic vessel network, in contrast to other reported models requiring several weeks. Coculture of lymphatic endothelial cells with the appropriate supporting cells and intact PDGFR-β signaling proved essential for the lymphangiogenesis process. Additionally, subjecting the constructs to cyclic stretch enabled the creation of complex muscle tissue aligned with the lymphatic and blood vessel networks, more precisely biomimicking native tissue. Interestingly, the response of developing lymphatic vessels to tensile forces was different from that of blood vessels; while blood vessels oriented perpendicularly to the stretch direction, lymphatic vessels mostly oriented in parallel to the stretch direction. Implantation of the engineered lymphatic constructs into a mouse abdominal wall muscle resulted in anastomosis between host and implant lymphatic vasculatures, demonstrating the engineered construct's potential functionality in vivo. Overall, this model provides a potential platform for investigating lymphangiogenesis and lymphatic disease mechanisms.
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http://dx.doi.org/10.1073/pnas.2101931118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8346860PMC
August 2021
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