Publications by authors named "Ming Yi"

283 Publications

Suppression of ventral hippocampal CA1 pyramidal neuronal activities enhances water intake.

Am J Physiol Cell Physiol 2021 Oct 27. Epub 2021 Oct 27.

Neuroscience Research Institute and Department of Neurobiology, School of Basic Medical Sciences, Peking University, Beijing, China.

Thirst is an important interoceptive response and drives water consumption. The hippocampus actively modulates food intake and energy metabolism, but direct evidence for the exact role of the hippocampus in modulating drinking behaviors is lacking. We observed decreased number of c-Fos-positive neurons in the ventral hippocampal CA1 (vCA1) after water restriction or hypertonic saline injection in rats. Suppressed vCA1 neuronal activities under the hypertonic state were further confirmed with in vivo electrophysiological recording and the level of suppression paralleled both the duration and the total amount of water consumption. Chemogenetic inhibition of vCA1 pyramidal neurons increased water consumption in rats injected with both normal and hypertonic saline. These findings suggest that suppression of vCA1 pyramidal neuronal activities enhances water intake.
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http://dx.doi.org/10.1152/ajpcell.00211.2021DOI Listing
October 2021

Roles of Microvesicles in Tumor Progression and Clinical Applications.

Int J Nanomedicine 2021 18;16:7071-7090. Epub 2021 Oct 18.

Department of Oncology, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, People's Republic of China.

Microvesicles are extracellular vesicles with diameter ranging from 100 to 1000 nm that are secreted by tumor cells or other cells in the tumor microenvironment. A growing number of studies demonstrate that tumor-derived microvesicles are involved in tumor initiation and progression, as well as drug resistance. In addition, tumor-derived microvesicles carry a variety of immunogenic molecules and inhibit tumor response to immunotherapy; therefore, they can be exploited for use in tumor vaccines. Moreover, because of their high stability, tumor-derived microvesicles extracted from body fluids can be used as biomarkers for cancer diagnosis or assessment of prognosis. Tumor-derived microvesicles can also be deployed to reverse drug resistance of tumor regenerative cells, or to deliver chemotherapeutic drugs and oncolytic adenovirus for the treatment of cancer patients. This review summarizes the general characteristics of tumor-derived microvesicles, focusing on their biological characteristics, their involvement in tumor progression, and their clinical applications.
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http://dx.doi.org/10.2147/IJN.S325448DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8536885PMC
October 2021

Development and validation of a nomogram for predicting albumin transfusion after spinal tuberculosis surgery: based on propensity score matching analysis.

World Neurosurg 2021 Oct 15. Epub 2021 Oct 15.

Spine and osteopathy ward, Guangxi Medical University First Affiliated Hospital, Nanning, Guangxi Province, China. Electronic address:

Background: There were few literature reports on the use of perioperative parameters to predict the risk of albumin transfusion after spinal tuberculosis surgery based on the application of nomogram and propensity score matching (PSM) analysis.

Purpose: The purpose was to predict the risk of albumin transfusion after spinal tuberculosis surgery based on a combination of PSM and nomogram.

Methods: The clinical data of the patients were collected in our hospital, including preoperative clinical data, preoperative laboratory tests, and postoperative clinical data. All data were divided into two groups, including the albumin transfusion group and the non-albumin transfusion group. The PSM analysis was used to adjust the baseline data of the two groups. The nomogram was further constructed. The practicability and predictive ability of the model were evaluated.

Results: A total of 494 cases were collected in this article. 102 pairs by PSM analysis were used to construct the nomogram. There were statistical differences in surgical approach, aspartate aminotransferase (AST)/ alanine aminotransferase (ALT), drainage, and kyphosis by logistic analysis, and these parameters were included in the construction of the nomogram. the C-index of the prediction model was 0.734. The area under the curve (AUC) was 0.73 and the net benefit was between 0.13 and 0.99. The calculated C-index was 0.71 by the internal verification method.

Conclusion: The PSM analysis had a good matching effect and the nomogram had a good predictive ability. Surgical approach, AST/ ALT, drainage, and kyphosis might be predictors of albumin transfusion after spinal tuberculosis surgery.
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http://dx.doi.org/10.1016/j.wneu.2021.10.102DOI Listing
October 2021

The Formation of 14H-LPSO in Mg-9Gd-2Y-2Zn-0.5Zr Alloy during Heat Treatment.

Materials (Basel) 2021 Oct 2;14(19). Epub 2021 Oct 2.

College of Materials Science and Engineering, North University of China, Taiyuan 030051, China.

There is a new long-period stacking ordered structure in Mg-RE-Zn magnesium alloys, namely the LPSO phase, which can effectively improve the yield strength, elongation, and corrosion resistance of Mg alloys. According to different types of Mg-RE-Zn alloy systems, two transformation modes are involved in the heat treatment transformation process. The first is the alloy without LPSO phase in the as-cast alloy, and the MgRE phase changes to 14H-LPSO phase. The second is the alloy containing LPSO phase in the as-cast state, and the 14H-LPSO phase is obtained by the transformations of 6H, 18R, and 24R. The effects of different solution parameters on the second phase of Mg-9Gd-2Y-2Zn-0.5Zr alloy were studied by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (XRD). The precipitation mechanism of 14H-LPSO phase during solution treatment was further clarified. At a solution time of 13 h, the grain size increased rapidly initially and then decreased slightly with increasing solution temperature. The analysis of the volume fraction of the second phase and lattice constant showed that Gd and Y elements in the alloy precipitated from the matrix and formed 14H-LPSO phase after solution treatment at 490 °C for 13 h. At this time, the hardness of the alloy reached the maximum of 74.6 HV. After solution treatment at 500 °C for 13 h, the solid solution degree of the alloy increases, and the grain size and hardness of the alloy remain basically unchanged.
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http://dx.doi.org/10.3390/ma14195758DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8510205PMC
October 2021

Environmental migration effects of air pollution: Micro-level evidence from China.

Environ Pollut 2021 Sep 30;292(Pt A):118263. Epub 2021 Sep 30.

School of Economics and Management, China University of Geosciences, Wuhan, PR China.

The willingness of migrating due to air pollution is widespread in China. However, there is a lack of direct evidence and discussion regarding whether this willingness has been translated into action. In this study, PM2.5 concentrations were used to represent air pollution in each city and were compared with individual migration data from the China Labor-force Dynamics Survey (CLDS) to examine population migration effects caused by air pollution. This study showed that (1) Population migration between Chinese cities shows sensitivity to air pollution, and air pollution increases the probability of moving away for local population. This finding is held under multiple robustness and endogeneity tests. (2) Population migration effects caused by air pollution were more pronounced among women, middle-aged people, those with lower educational levels, from agricultural households, Han Chinese groups, and populations in southern cities. (3) The use of individual self-rated health data verified that physical health is an important channel through which individual migration decisions are influenced by air pollution, the older an individual, the more his or her health was affected. In light of these findings, this study led to conclusions regarding targeted policy recommendations in terms of talent clustering, social equity, and demographic balance.
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http://dx.doi.org/10.1016/j.envpol.2021.118263DOI Listing
September 2021

Preoperative aspartate transaminase/alanine transaminase ratio as a prognostic biomarker in primary non-muscle-invasive bladder cancer: a propensity score-matched study.

BMC Urol 2021 Sep 27;21(1):136. Epub 2021 Sep 27.

Department of Urology, The First Affiliated Hospital of Nanchang University, Nanchang City, 330000, Jiangxi Province, China.

Purpose: To evaluate the prognostic value of the aspartate transaminase/alanine transaminase (AST/ALT) ratio in primary non-muscle-invasive bladder cancer (NMIBC) using propensity score matching (PSM) analysis.

Methods: We retrospectively collected the clinical and pathological data from 314 patients with primary NMIBC who underwent transurethral resection of bladder tumor. The full cohorts were divided into a low AST/ALT ratio group and a high AST/ALT ratio group according to the optimal cut-off value which was obtained based on the analysis of the receiver operating characteristic curve for the 3-year recurrence-free survival (RFS). After 1:1 PSM, the correlation between preoperative AST/ALT ratio and survival prognosis was evaluated by Kaplan-Meier analysis with log-rank tests. The independent prognostic factors for RFS and progression-free survival (PFS) were also analyzed.

Results: The optimum cutoff value of the preoperative AST/ALT ratio was 1.40. Before PSM, a high AST/ALT ratio was correlated with the larger proportion of age > 60 years (P = 0.007) and the worse pathological T stage (P < 0.001). After PSM, patients with a high AST/ALT ratio had poorer RFS and PFS than patients with a low AST/ALT ratio (all P < 0.001). In addition, multivariate Cox regression analysis indicated that preoperative AST/ALT ratio was considered as an independent prognostic factor of RFS (HR 2.865; 95%CI 1.873-4.381; P < 0.001) and PFS (HR 4.771; 95%CI 2.607-8.734; P < 0.001) in patients with primary NMIBC.

Conclusions: The high AST/ALT ratio group tended to have poorer RFS and PFS than the low AST/ALT ratio group. Our results also indicated that the elevated preoperative AST/ALT ratio could be seen as a useful prognostic biomarker for predicting early disease recurrence and progression in patients with primary NMIBC.
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http://dx.doi.org/10.1186/s12894-021-00901-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8474786PMC
September 2021

Whether green technology innovation is conducive to haze emission reduction: empirical evidence from China.

Environ Sci Pollut Res Int 2021 Sep 24. Epub 2021 Sep 24.

Freeman School of Business, Tulane University, New Orleans, LA, USA.

With the acceleration of industrialization, haze pollution has become a severe environmental pollution problem, and green technology innovation is one feasible way to alleviate it. Based on the PM concentration data of 30 provinces in mainland China from 2011 to 2017, we use a spatial panel model to investigate the spatial characteristics of haze pollution and examine the impact of green technology innovation on it. Results show that haze pollution has spatial correlation and a time lag. Its spatial correlation is associated with geographical distance as well as the compound influence of distance and economic development. Green technology innovation and foreign investment have inhibitory and negative spillover effects on haze pollution. Industrial structure and energy consumption structure play a partial intermediary role between green technology innovation and haze pollution, and the former has a significant negative spillover, while the latter has a positive effect. To reduce haze pollution, China should improve the level of green technology innovation, use foreign investment wisely, and enhance policy support and guidance. It should also promote the rationalization of industrial structure, optimize energy structure, and implement energy substitution. Finally, it is crucial that it should strengthen regional collaborative governance and build a multi-agent governance system.
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http://dx.doi.org/10.1007/s11356-021-16467-wDOI Listing
September 2021

Combine and conquer: manganese synergizing anti-TGF-β/PD-L1 bispecific antibody YM101 to overcome immunotherapy resistance in non-inflamed cancers.

J Hematol Oncol 2021 Sep 15;14(1):146. Epub 2021 Sep 15.

Department of Oncology, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan, 430030, People's Republic of China.

Background: Our previous work showed that the anti-TGF-β/PD-L1 bispecific antibody YM101 effectively overcame anti-PD-L1 resistance in immune-excluded tumor models. However, in immune-desert models, the efficacy of YM101 was limited. Bivalent manganese (Mn) is identified as a natural stimulator of interferon genes (STING) agonist, which might enhance cancer antigen presentation and improve the therapeutic effect of YM101.

Methods: The effect of Mn on STING pathway was validated by western blotting and enzyme-linked immunosorbent assay. Dendritic cell (DC) maturation was measured by flow cytometry. The synergistic effect between Mn and YM101 in vitro was determined by one-way mixed lymphocyte reaction, CFSE dilution assay, and cytokine detection. The in vivo antitumor effect of Mn plus YM101 therapy was assessed in CT26, EMT-6, H22, and B16 tumor models. Flow cytometry, RNA-seq, and immunofluorescent staining were adopted to investigate the alterations in the tumor microenvironment.

Results: Mn could activate STING pathway and promote the maturation of human and murine DC. The results of one-way mixed lymphocyte reaction showed that Mn synergized YM101 in T cell activation. Moreover, in multiple syngeneic murine tumor models, Mn plus YM101 therapy exhibited a durable antitumor effect and prolonged the survival of tumor-bearing mice. Relative to YM101 monotherapy and Mn plus anti-PD-L1 therapy, Mn plus YM101 treatment had a more powerful antitumor effect and a broader antitumor spectrum. Mechanistically, Mn plus YM101 strategy simultaneously regulated multiple components in the antitumor immunity and drove the shift from immune-excluded or immune-desert to immune-inflamed tumors. The investigation in the TME indicated Mn plus YM101 strategy activated innate and adaptive immunity, enhanced cancer antigen presentation, and upregulated the density and function of tumor-infiltrating lymphocytes. This normalized TME and reinvigorated antitumor immunity contributed to the superior antitumor effect of the combination therapy.

Conclusion: Combining Mn with YM101 has a synergistic antitumor effect, effectively controlling tumor growth and prolonging the survival of tumor-bearing mice. This novel cocktail strategy has the potential to be a universal regimen for inflamed and non-inflamed tumors.
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http://dx.doi.org/10.1186/s13045-021-01155-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8442312PMC
September 2021

Highly porous nanofiber-supported monolayer graphene membranes for ultrafast organic solvent nanofiltration.

Sci Adv 2021 Sep 8;7(37):eabg6263. Epub 2021 Sep 8.

Department of Chemical and Biomolecular Engineering, National University of Singapore, Singapore 117576, Singapore.

[Figure: see text].
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http://dx.doi.org/10.1126/sciadv.abg6263DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8442935PMC
September 2021

SERS-based lateral flow immunoassay for sensitive and simultaneous detection of anti-SARS-CoV-2 IgM and IgG antibodies by using gap-enhanced Raman nanotags.

Sens Actuators B Chem 2021 Dec 3;348:130706. Epub 2021 Sep 3.

College of Electrical and Electronic Engineering, Wenzhou University, Wenzhou 325035, PR China.

The lateral flow immunoassay (LFIA) has played a crucial role in early diagnosis during the current COVID-19 pandemic owing to its simplicity, speed and affordability for coronavirus antibody detection. However, the sensitivity of the commercially available LFIAs needs to be improved to better prevent the spread of the infection. Here, we developed an ultra-sensitive surface-enhanced Raman scattering-based lateral flow immunoassay (SERS-based LFIA) strip for simultaneous detection of anti-SARS-CoV-2 IgM and IgG by using gap-enhanced Raman nanotags (GERTs). The GERTs with a 1 nm gap between the core and shell were used to produce the "hot spots", which provided about 30-fold enhancement as compared to conventional nanotags. The COVID-19 recombinant antigens were conjugated on GERTs surfaces and replaced the traditional colloidal gold for the Raman sensitive detection of human IgM and IgG. The LODs of IgM and IgG were found to be 1 ng/mL and 0.1 ng/mL (about 100 times decrease was observed as compared to commercially available LFIA strips), respectively. Moreover, under the condition of common nano-surface antigen, precise SERS signals proved the unreliability of quantitation because of the interference effect of IgM on IgG.
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http://dx.doi.org/10.1016/j.snb.2021.130706DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8413105PMC
December 2021

PlantMirP2: An Accurate, Fast and Easy-To-Use Program for Plant Pre-miRNA and miRNA Prediction.

Genes (Basel) 2021 Aug 21;12(8). Epub 2021 Aug 21.

School of Mathematics and Physics, China University of Geosciences, Wuhan 430074, China.

MicroRNAs (miRNAs) are a kind of short non-coding ribonucleic acid molecules that can regulate gene expression. The computational identification of plant miRNAs is of great significance to understanding biological functions. In our previous studies, we have put firstly forward and further developed a set of knowledge-based energy features to construct two plant pre-miRNA prediction tools (plantMirP and riceMirP). However, these two tools cannot be used for miRNA prediction from NGS (Next-Generation Sequencing) data. In addition, for further improving the prediction performance and accessibility, plantMirP2 has been developed. Based on the latest dataset, plantMirP2 achieves a promising performance: 0.9968 (Area Under Curve, AUC), 0.9754 (accuracy), 0.9675 (sensitivity) and 0.9876 (specificity). Additionally, the comparisons with other plant pre-miRNA tools show that plantMirP2 performs better. Finally, the webserver and stand-alone version of plantMirP2 are available.
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http://dx.doi.org/10.3390/genes12081280DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8392394PMC
August 2021

Ccdc134 deficiency impairs cerebellar development and motor coordination.

Genes Brain Behav 2021 Sep 23;20(7):e12763. Epub 2021 Aug 23.

Department of Immunology, School of Basic Medical Sciences, Peking University, and NHC Key Laboratory of Medical Immunology (Peking University), Beijing, China.

Coiled-coil domain containing 134 (CCDC134) has been shown to serve as an immune cytokine to exert antitumor effects and to act as a novel regulator of hADA2a to affect PCAF acetyltransferase activity. While Ccdc134 loss causes abnormal brain development in mice, the significance of CCDC134 in neuronal development in vivo is controversial. Here, we report that CCDC134 is highly expressed in Purkinje cells (PCs) at all developmental stages and regulates mammalian cerebellar development in a cell type-specific manner. Selective deletion of Ccdc134 in mouse neural stem cells (NSCs) caused defects in cerebellar morphogenesis, including a decrease in the number of PCs and impairment of PC dendritic growth, as well as abnormal granule cell development. Moreover, loss of Ccdc134 caused progressive motor dysfunction with deficits in motor coordination and motor learning. Finally, Ccdc134 deficiency inhibited Wnt signaling but increased Ataxin1 levels. Our findings provide evidence that CCDC134 plays an important role in cerebellar development, possibly through regulating Wnt signaling and Ataxin1 expression levels, and in controlling cerebellar function for motor coordination and motor learning, ultimately making it a potential contributor to cerebellar pathogenesis.
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http://dx.doi.org/10.1111/gbb.12763DOI Listing
September 2021

Advances of Targeted Therapy for Hepatocellular Carcinoma.

Front Oncol 2021 26;11:719896. Epub 2021 Jul 26.

Department of Oncology, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Hepatocellular carcinoma (HCC) is one of the common and fatal malignancies, which is a significant global health problem. The clinical applicability of traditional surgery and other locoregional therapies is limited, and these therapeutic strategies are far from satisfactory in improving the outcomes of advanced HCC. In the past decade, targeted therapy had made a ground-breaking progress in advanced HCC. Those targeted therapies exert antitumor effects through specific signals, including anti-angiogenesis or cell cycle progression. As a standard systemic therapy option, it tremendously improves the survival of this devastating disease. Moreover, the combination of targeted therapy with immune checkpoint inhibitor (ICI) has demonstrated more potent anticancer effects and becomes the hot topic in clinical studies. The combining medications bring about a paradigm shift in the treatment of advanced HCC. In this review, we presented all approved targeted agents for advanced HCC with an emphasis on their clinical efficacy, summarized the advances of multi-target drugs in research for HCC and potential therapeutic targets for drug development. We also discussed the exciting results of the combination between targeted therapy and ICI.
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http://dx.doi.org/10.3389/fonc.2021.719896DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8350567PMC
July 2021

Multi-Level Analyses of Genome-Wide Association Study to Reveal Significant Risk Genes and Pathways in Neuromyelitis Optica Spectrum Disorder.

Front Genet 2021 21;12:690537. Epub 2021 Jul 21.

Department of Neurology, Tianjin Neurological Institute, Tianjin Medical University General Hospital, Tianjin, China.

Background: Neuromyelitis optica spectrum disorder (NMOSD) is an inflammatory disease of the central nervous system and it is understandable that environmental and genetic factors underlie the etiology of NMOSD. However, the susceptibility genes and associated pathways of NMOSD patients who are AQP4-Ab positive and negative have not been elucidated.

Methods: Secondary analysis from a NMOSD Genome-wide association study (GWAS) dataset originally published in 2018 (215 NMOSD cases and 1244 controls) was conducted to identify potential susceptibility genes and associated pathways in AQP4-positive and negative NMOSD patients, respectively (132 AQP4-positive and 83 AQP4-negative).

Results: In AQP4-positive NMOSD cases, five shared risk genes were obtained at chromosome 6 in AQP4-positive NMOSD cases by using more stringent -Values in both methods ( < 0.05/16,532), comprising CFB, EHMT2, HLA-DQA1, MSH5, and SLC44A4. Fifty potential susceptibility gene sets were determined and 12 significant KEGG pathways were identified. Sixty-seven biological process pathways, 32 cellular-component pathways, and 29 molecular-function pathways with a -Value of <0.05 were obtained from the GO annotations of the 128 pathways identified. In the AQP4 negative NMOSD group, no significant genes were obtained by using more stringent -Values in both methods ( < 0.05/16,485). The 22 potential susceptibility gene sets were determined. There were no shared potential susceptibility genes between the AQP4-positive and negative groups, furthermore, four significant KEGG pathways were also identified. Of the GO annotations of the 165 pathways identified, 99 biological process pathways, 37 cellular-component pathways, and 29 molecular-function pathways with a -Value of <0.05 were obtained.

Conclusion: The potential molecular mechanism underlying NMOSD may be related to proteins encoded by these novel genes in complements, antigen presentation, and immune regulation. The new results may represent an improved comprehension of the genetic and molecular mechanisms underlying NMOSD.
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http://dx.doi.org/10.3389/fgene.2021.690537DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8335167PMC
July 2021

Epidemiological trends of women's cancers from 1990 to 2019 at the global, regional, and national levels: a population-based study.

Biomark Res 2021 Jul 7;9(1):55. Epub 2021 Jul 7.

Department of Oncology, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, 430030, Wuhan, China.

Background: Every year around the world, more than 2 million women are diagnosed with breast cancer and genital tract cancers. However, there are rare studies comprehensively describing the global and regional trends of incidence and mortality of women's cancers.

Methods: To study the burden and trend of women's cancers, we conducted this cross-sectional study based on the epidemiologic data of Global Burden of Disease 2019. In this study, female patients with breast cancer, cervical cancer, ovarian cancer, and uterine cancer worldwide from 1990 to 2019 were involved. The incidence, death, and disability-adjusted life-year (DALY) were used to measure the outcomes of women's cancers. The estimated annual percentage change (EAPC) was calculated to assess the changing trend of cancer burden.

Results: Among the four women's cancers, the burden of female breast cancer was highest. During the past 30 years, the incidence, death, and DALY of female breast cancer kept increasing worldwide. In most regions especially developing countries, cervical cancer was the second most common women's cancer. At the same time, ovarian cancer and uterine cancer occurred less frequently. Generally, the age-standardized incidence rates (ASIRs) of breast cancer, ovarian cancer, and uterine cancer were positively correlated to sociodemographic index (SDI) value. In contrast, the ASIR of cervical cancer was negatively correlated to SDI value.

Conclusions: Our study indicates that the incidence and mortality of women's cancers have geographical variations and change along with SDI value. The results might be helpful to policy-makers to allocate healthy resources to control women's cancers.
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http://dx.doi.org/10.1186/s40364-021-00310-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261911PMC
July 2021

Glycolysis- and immune-related novel prognostic biomarkers of Ewing's sarcoma: glucuronic acid epimerase and triosephosphate isomerase 1.

Aging (Albany NY) 2021 07 7;13(13):17516-17535. Epub 2021 Jul 7.

The First Clinical Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Province, China.

Introduction: Owing to the poor prognosis of Ewing's sarcoma, reliable prognostic biomarkers are highly warranted for clinical diagnosis of the disease.

Materials And Methods: A combination of the weighted correlation network analysis and differentially expression analysis was used for initial screening; glycolysis-related genes were extracted and subjected to univariate Cox, LASSO regression, and multivariate Cox analyses to construct prognostic models. The immune cell composition of each sample was analysed using CIBERSORT software. Immunohistochemical analysis was performed for assessing the differential expression of modelled genes in Ewing's sarcoma and paraneoplastic tissues.

Results: A logistic regression model constructed for the prognosis of Ewing's sarcoma exhibited that the patient survival rate in the high-risk group is much lower than in the low-risk group. CIBERSORT analysis exhibited a strong correlation of Ewing's sarcoma with naïve B cells, CD8 T cells, activated NK cells, and M0 macrophages (P < 0.05). Immunohistochemical analysis confirmed the study findings.

Conclusions: and can be used as prognostic biomarkers to predict the prognosis of Ewing's sarcoma, and a close association of Ewing's sarcoma with naïve B cells, CD8 T cells, activated NK cells, and M0 macrophages provides a novel approach to the disease immunotherapy.
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http://dx.doi.org/10.18632/aging.203242DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8312448PMC
July 2021

Structural, magnetic, and electronic evolution of the spin-ladder system BaFeS Se with isoelectronic substitution.

Phys Rev B 2020 Jun;101(23)

Materials Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA.

We report experimental studies of a series of BaFeS Se (0 ⩽ ⩽ 3) single crystals and powder specimens using x-ray diffraction, neutron-diffraction, muon-spin-relaxation, and electrical transport measurements. A structural transformation from (BaFeS) to (BaFeSe) was identified around = 0.7 - 1. Neutron-diffraction measurements on the samples with = 0.2, 0.4, and 0.7 reveal that the Néel temperature of the stripe antiferromagnetic order is gradually suppressed from ~120 to 85 K, while the magnitude of the ordered Fe moments shows very little variation. Similarly, the block antiferromagnetic order in BaFeSe remains robust for 1.5 ⩽ ⩽ 3 with negligible variation in the ordered moment and a slight decrease of the Néel temperature from 250 K ( = 3) to 225 K ( = 1.5). The sample with = 1 near the and border shows coexisting, two-dimensional, short-range stripe- and block-type antiferromagnetic correlations. The system remains insulating for all , but the thermal activation gap shows an abrupt increase when traversing the boundary from the stripe phase to the block phase. The results demonstrate that the crystal structure, magnetic order, and electronic properties are strongly coupled in the BaFeS Se system.
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http://dx.doi.org/10.1103/PhysRevB.101.235134DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8204408PMC
June 2020

A transport channel-regulated MXene membrane organic phosphonic acids for efficient water permeation.

Chem Commun (Camb) 2021 Jun;57(51):6245-6248

Department of Materials and Environmental Chemistry, Stockholm University, Stockholm, 10691, Sweden.

A series of organic phosphonic acids (OPAs) were applied as multifunctional spacers to enlarge the inner space of carbide MXene (Ti3C2Tx) laminates. A synergistic improvement in permeance, rejection and stability is achieved via introducing OPA to create pillared laminates. This strategy provides a universal way to regulate transport channels of MXene-based membranes.
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http://dx.doi.org/10.1039/d1cc01464aDOI Listing
June 2021

The prevalence of anti-neurofascin-155 antibodies in patients with neuromyelitis optica spectrum disorders.

Clin Exp Immunol 2021 Oct 7;206(1):1-11. Epub 2021 Jun 7.

Department of Neurology, Tianjin Neurological Institute, Tianjin Medical University General Hospital, Tianjin, China.

Anti-neurofascin-155 (NF155) antibodies have been observed in two cases with neuromyelitis optica spectrum disorders (NMOSD). This study investigated the prevalence of anti-NF155 antibodies in patients with NMOSD and the clinical features of anti-NF155 antibody-positive patients. Sera from 129 patients with NMOSD were screened with anti-NF155 antibodies by cell-based assay (CBA) and re-examined using immunostaining of teased mouse sciatic nerve fibres. Fifty-six patients with multiple sclerosis (MS) and 50 healthy controls (HC) were also enrolled for detecting anti-NF155 antibodies. A total of 12.40% (16 of 129) of patients with NMOSD were positive for anti-NF155 antibodies confirmed by both CBA and immunostaining. Immunoglobulin (Ig) G1 was the predominant subclass. However, none of 56 MS patients or 50 HC were positive for anti-NF155 antibodies. Anti-NF155 antibody-positive NMOSD patients had a higher proportion of co-existing with autoimmune diseases (p < 0.001) and higher positive rates of serum non-organ-specific autoantibodies, including anti-SSA antibodies (p < 0.001), anti-SSB antibodies (p = 0.008), anti-Ro-52 antibodies (p < 0.001) and rheumatoid factor (p < 0.001). Five anti-NF155 antibody-positive NMOSD patients who took part in the nerve conduction study showed mildly abnormal results. Differences in some nerve conduction study parameters were observed between anti-NF155 antibody-positive and negative patients. Anti-NF155 antibodies occurred in a small proportion of NMOSD patients. Anti-NF155 antibody-positive NMOSD patients tended to co-exist with autoimmune diseases.
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http://dx.doi.org/10.1111/cei.13617DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8446398PMC
October 2021

TRIM68, PIKFYVE, and DYNLL2: The Possible Novel Autophagy- and Immunity-Associated Gene Biomarkers for Osteosarcoma Prognosis.

Front Oncol 2021 22;11:643104. Epub 2021 Apr 22.

Department of Spinal Orthopedics, The First Clinical Affiliated Hospital of Guangxi Medical University, Nanning, China.

Introduction: Osteosarcoma is among the most common orthopedic neoplasms, and currently, there are no adequate biomarkers to predict its prognosis. Therefore, the present study was aimed to identify the prognostic biomarkers for autophagy-and immune-related osteosarcoma using bioinformatics tools for guiding the clinical diagnosis and treatment of this disease.

Materials And Methods: The gene expression and clinical information data were downloaded from the Public database. The genes associated with autophagy were extracted, followed by the development of a logistic regression model for predicting the prognosis of osteosarcoma using univariate and multivariate COX regression analysis and LASSO regression analysis. The accuracy of the constructed model was verified through the ROC curves, calibration plots, and Nomogram plots. Next, immune cell typing was performed using CIBERSORT to analyze the expression of the immune cells in each sample. For the results obtained from the analysis, we used qRT-PCR validation in two strains of human osteosarcoma cells.

Results: The screening process identified a total of three genes that fulfilled all the screening criteria. The survival curves of the constructed prognostic model revealed that patients with the high risk presented significantly lower survival than the patients with low risk. Finally, the immune cell component analysis revealed that all three genes were significantly associated with the immune cells. The expressions of TRIM68, PIKFYVE, and DYNLL2 were higher in the osteosarcoma cells compared to the control cells. Finally, we used human pathological tissue sections to validate the expression of the genes modeled in osteosarcoma and paracancerous tissue.

Conclusion: The TRIM68, PIKFYVE, and DYNLL2 genes can be used as biomarkers for predicting the prognosis of osteosarcoma.
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http://dx.doi.org/10.3389/fonc.2021.643104DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8101494PMC
April 2021

The biology of combination immunotherapy in recurrent metastatic head and neck cancer.

Int J Biochem Cell Biol 2021 07 4;136:106002. Epub 2021 May 4.

Department of Oncology, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China; Department of Medical Oncology, The Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer Hospital, Zhengzhou, China. Electronic address:

Preclinical data suggest that head and neck cancer is an intrinsically immunosuppressive disease with abnormal inflammatory components in the tumor microenvironment. The development of immune checkpoint inhibitors, which are monoclonal antibodies capable of inhibiting immune suppressive signals to prime anticancer immunity, has revolutionized the therapeutic landscape in recurrent/metastatic head and neck cancer. However, patients with head and neck cancer present primary resistance to immunotherapy. Many ongoing trials include combinations of immunotherapy with different therapeutic interventions, aiming to improve response rates and overall survival. As novel therapy strategies are leveraged, the significance of immunotherapy in recurrent/metastatic head and neck cancer continues to be revealed. This review aims to summarize combinational immunotherapy in head and neck cancer.
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http://dx.doi.org/10.1016/j.biocel.2021.106002DOI Listing
July 2021

Sensitivity of Oncogenic KRAS-Expressing Cells to CDK9 Inhibition.

SLAS Discov 2021 Aug 24;26(7):922-932. Epub 2021 Apr 24.

National Cancer Institute (NCI) RAS Initiative, Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, Leidos Biomedical Research, Frederick, MD, USA.

Oncogenic forms of KRAS proteins are known to be drivers of pancreatic, colorectal, and lung cancers. The goal of this study is to identify chemical leads that inhibit oncogenic KRAS signaling. We first developed an isogenic panel of mouse embryonic fibroblast (MEF) cell lines that carry wild-type RAS, oncogenic KRAS, and oncogenic BRAF. We validated these cell lines by screening against a tool compound library of 1402 annotated inhibitors in an adenosine triphosphate (ATP)-based cell viability assay. Subsequently, this MEF panel was used to conduct a high-throughput phenotypic screen in a cell viability assay with a proprietary compound library. All 126 compounds that exhibited a selective activity against mutant KRAS were selected and prioritized based on their activities in secondary assays. Finally, five chemical clusters were chosen. They had specific activity against SW620 and LS513 over Colo320 colorectal cancer cell lines. In addition, they had no effects on BRAF, MEK1, extracellular signal-regulated kinase 2 (ERK2), phosphoinositide 3-kinase alpha (PI3Kα), AKT1, or mammalian target of rapamycin (mTOR) as tested in in vitro enzymatic activity assays. Biophysical assays demonstrated that these compounds did not bind directly to KRAS. We further identified the mechanism of action and showed that three of them have CDK9 inhibitory activity. In conclusion, we have developed and validated an isogenic MEF panel that was used successfully to identify RAS oncogenic or wild-type allele-specific vulnerabilities. Furthermore, we identified sensitivity of oncogenic KRAS-expressing cells to CDK9 inhibitors, which warrants future studies of treating KRAS-driven cancers with CDK9 inhibitors.
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http://dx.doi.org/10.1177/24725552211008853DOI Listing
August 2021

Short-Range Nematic Fluctuations in Sr_{1-x}Na_{x}Fe_{2}As_{2} Superconductors.

Phys Rev Lett 2021 Mar;126(10):107001

Department of Physics, University of California, Berkeley, California 94720, USA.

Interactions between nematic fluctuations, magnetic order and superconductivity are central to the physics of iron-based superconductors. Here we report on in-plane transverse acoustic phonons in hole-doped Sr_{1-x}Na_{x}Fe_{2}As_{2} measured via inelastic x-ray scattering, and extract both the nematic susceptibility and the nematic correlation length. By a self-contained method of analysis, for the underdoped (x=0.36) sample, which harbors a magnetically ordered tetragonal phase, we find it hosts a short nematic correlation length ξ∼10  Å and a large nematic susceptibility χ_{nem}. The optimal-doped (x=0.55) sample exhibits weaker phonon softening effects, indicative of both reduced ξ and χ_{nem}. Our results suggest short-range nematic fluctuations may favor superconductivity, placing emphasis on the nematic correlation length for understanding the iron-based superconductors.
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http://dx.doi.org/10.1103/PhysRevLett.126.107001DOI Listing
March 2021

Cometabolism of 17α-ethynylestradiol by nitrifying bacteria depends on reducing power availability and leads to elevated nitric oxide formation.

Environ Int 2021 08 25;153:106528. Epub 2021 Mar 25.

Key Laboratory of Environment Remediation and Ecological Health, Ministry of Education, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China. Electronic address:

17α-ethynylestradiol (EE2) is a priority emerging contaminant (EC) in diverse environments that can be cometabolized by ammonia oxidizing bacteria (AOB). However, its transformation kinetics and the underlying molecular mechanism are unclear. In this study, kinetic parameters, including maximum specific EE2 transformation rate, EE2 half-saturation coefficient, and EE2transformation capacity of AOBwere obtained by using the model AOB strain, Nitrosomonas europaea 19718. The relationship between EE2 cometabolism and ammonia oxidation was divided into three phases according to reducing power availability, namely "activation", "coupling", and "saturation". Specifically, there was a universal lag of EE2 transformation after ammonia oxidation was initiated, suggesting that sufficient reducing power (approximately 0.95 ± 0.06 mol NADH/L) was required to activate EE2 cometabolism. Interestingly, nitric oxide emission increased by 12 ± 2% during EE2 cometabolism, along with significantly upregulated nirK cluster genes. The findings are of importance to understanding the cometabolic behavior and mechanism of EE2 in natural and engineered environments. Maintaining relatively high and stable reducing power supply from ammonia oxidation can potentially improve the cometabolic removal of EE2 and other ECs during wastewater nitrification processes.
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http://dx.doi.org/10.1016/j.envint.2021.106528DOI Listing
August 2021

Predictive biomarkers of anti-PD-1/PD-L1 therapy in NSCLC.

Exp Hematol Oncol 2021 Mar 2;10(1):18. Epub 2021 Mar 2.

Department of Medical Oncology, The Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer Hospital, Zhengzhou, 450008, China.

Immunotherapy, especially anti-programmed cell death protein 1/programmed cell death ligand 1 (PD-1/PD-L1) treatment has significantly improved the survival of non-small cell lung cancer (NSCLC) patients. However, the overall response rate remains unsatisfactory. Many factors affect the outcome of anti-PD-1/PD-L1 treatment, such as PD-L1 expression level, tumor-infiltrating lymphocytes (TILs), tumor mutation burden (TMB), neoantigens, and driver gene mutations. Further exploration of biomarkers would be favorable for the best selection of patients and precisely predict the efficacy of anti-PD-1/PD-L1 treatment. In this review, we summarized the latest advances in this field, and discussed the potential applications of these laboratory findings in the clinic.
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http://dx.doi.org/10.1186/s40164-021-00211-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7923338PMC
March 2021

Assessing the Influence of COVID-19 on the Shortwave Radiative Fluxes Over the East Asian Marginal Seas.

Geophys Res Lett 2021 Feb 2;48(3):e2020GL091699. Epub 2021 Feb 2.

Department of Meteorology University of Reading, Reading Reading Whitenights UK.

The Coronavirus Disease 2019 (COVID-19) pandemic led to a widespread reduction in aerosol emissions. Using satellite observations and climate model simulations, we study the underlying mechanisms of the large decreases in solar clear-sky reflection (3.8 W m or 7%) and aerosol optical depth (0.16 W m or 32%) observed over the East Asian Marginal Seas in March 2020. By separating the impacts from meteorology and emissions in the model simulations, we find that about one-third of the clear-sky anomalies can be attributed to pandemic-related emission reductions, and the rest to weather variability and long-term emission trends. The model is skillful at reproducing the observed interannual variations in solar all-sky reflection, but no COVID-19 signal is discerned. The current observational and modeling capabilities will be critical for monitoring, understanding, and predicting the radiative forcing and climate impacts of the ongoing crisis.
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http://dx.doi.org/10.1029/2020GL091699DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7883069PMC
February 2021

A Retrospective Study of Factors Associated with Restoration of Thoracic Kyphosis in 43 Patients with Adolescent Idiopathic Scoliosis with Lenke Type 1 Curvature.

Med Sci Monit 2021 Feb 20;27:e929149. Epub 2021 Feb 20.

Department of Spine Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China (mainland).

BACKGROUND This retrospective study aimed to identify the factors associated with successful surgical correction of thoracic kyphosis (TK) in 43 patients with adolescent idiopathic scoliosis (AIS) with Lenke type 1 curvature, in which the major curve with the largest Cobb angle was mainly in the thoracic region. MATERIAL AND METHODS We collected data from patients with Lenke 1 AIS. The following parameters were measured: Cobb angle, side-bending Cobb angle, cervical lordosis (CL), TK, lumbar lordosis (LL), pelvic incidence (PI), sacral slope (SS), pelvic tilt (PT), the sagittal vertical axis (SVA), the center of a C7 plumb line to the center sacral vertical line (C7-CSVL), correction rate, Ponte osteotomy, flexibility, and screw density. Univariate analysis and multivariate logistic regression analyses were performed. RESULTS Among the 43 cases analyzed, the mean postoperative Cobb angle at the last follow-up, C7-CSVL, SVA, CL, TK, LL, PI, SS, and PT were respectively 21.33±9.47°, 10.41±8.45 mm, 19.68±14.33 mm, 16.19±7.45°, 23.12±7.45°, 50.33±11.37°, 49.70±9.83°, 39.42±8.11°, and 10.16±6.63°. Univariate analysis suggested that preoperative TK, preoperative LL, and Ponte osteotomy were statistically significant (P<0.05), and multivariate analysis suggested that preoperative LL and Ponte osteotomy were statistically significant (P<0.05). CONCLUSIONS The results of this study demonstrated that preoperative TK, preoperative LL, and Ponte osteotomy were related factors for maintaining normal TK. Multivariate analysis suggested that preoperative LL and the use of Ponte osteotomy with full-thickness segmental resection of the spinal posterior column resulted in the successful surgical correction of TK in patients with AIS with Lenke type 1 curvature.
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http://dx.doi.org/10.12659/MSM.929149DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7903848PMC
February 2021

Elevated cerebrospinal fluid levels of beta-2-microglobulin in patients with Guillain-Barré syndrome and their correlations with clinical features.

Neurol Sci 2021 Oct 17;42(10):4249-4255. Epub 2021 Feb 17.

Department of Neurology, Tianjin Neurological Institute, Tianjin Medical University General Hospital, 154 Anshan Road, Heping District, Tianjin, 300052, China.

Backgrounds: Beta-2-microglobulin (β2-MG) levels vary in many infectious and autoimmune diseases. We investigated plasma and cerebrospinal fluid (CSF) β2-MG levels in patients with Guillain-Barré syndrome (GBS) and their correlations with clinical parameters.

Methods: CSF samples from 50 patients with GBS including 19 acute inflammatory demyelinating polyneuropathy (AIDP), 6 acute motor axonal neuropathy (AMAN), 10 acute motor-sensory axonal neuropathy (AMSAN), 7 Miller-Fisher syndrome (MFS), and 8 unclassified patients were collected. Moreover, 23 CSF samples from patients with non-inflammatory neurological disorders (NIND) as controls were collected. Plasma samples from 42 enrolled patients and 29 healthy individuals were also collected. The β2-MG levels were measured by immunoturbidimetry on automatic biochemical analyser. Besides, clinical data were extracted from electronic patient documentation system.

Results: CSF levels of β2-MG, lactate dehydrogenase (LDH), and lactate were significantly increased in patients with GBS (p = 0.004, p = 0.041, p = 0.040, respectively), particularly in patients with AIDP (p < 0.001, p = 0.001, p = 0.015, respectively), whereas no statistically significant difference was found in plasma levels of β2-MG. Furthermore, CSF levels of β2-MG were positively correlated with Hughes functional score (r = 0.493, p = 0.032), LDH (r = 0.796, p < 0.001), and lactate (r = 0.481, p = 0.037) but not with protein (r = - 0.090, p = 0.713) in AIDP patients.

Conclusions: CSF β2-MG levels may help identify AIDP and indicate clinical severity. CSF LDH and lactate levels correlate with CSF β2-MG levels; interaction among these biomarkers would need further investigation.
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http://dx.doi.org/10.1007/s10072-021-05108-2DOI Listing
October 2021

The construction, expression, and enhanced anti-tumor activity of YM101: a bispecific antibody simultaneously targeting TGF-β and PD-L1.

J Hematol Oncol 2021 02 16;14(1):27. Epub 2021 Feb 16.

Department of Oncology, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan, 430030, People's Republic of China.

Background: Therapeutic antibodies targeting programmed cell death protein 1 (PD-1)/programmed death-ligand 1 (PD-L1) axis induce potent and durable anti-tumor responses in multiple types of cancers. However, only a subset of patients benefits from anti-PD-1/PD-L1 therapies. As a negative regulator of anti-tumor immunity, TGF-β impairs the efficacy of anti-PD-1/PD-L1 and induces drug resistance. Developing a novel treatment strategy to simultaneously block PD-1/PD-L1 and TGF-β would be valuable to enhance the effect of anti-PD-1/PD-L1 and relieve drug resistance.

Methods: Based on the Check-BODY™ technology platform, we developed an anti-TGF-β/PD-L1 bispecific antibody YM101. The bioactivity of the anti-TGF-β moiety was determined by Smad-luciferase reporter assay, transwell assay, western blotting, CCK-8, and flow cytometry. The bioactivity of the anti-PD-L1 moiety was measured by T cell activation assays. EMT-6, CT26, and 3LL tumor models were used to investigate the anti-tumor activity of YM101 in vivo. RNA-seq, immunohistochemical staining, and flow cytometry were utilized to analyze the effect of YM101 on the tumor microenvironment.

Results: YM101 could bind to TGF-β and PD-L1 specifically. In vitro experiments showed that YM101 effectively counteracted the biological effects of TGF-β and PD-1/PD-L1 pathway, including activating Smad signaling, inducing epithelial-mesenchymal transition, and immunosuppression. Besides, in vivo experiments indicated the anti-tumor activity of YM101 was superior to anti-TGF-β and anti-PD-L1 monotherapies. Mechanistically, YM101 promoted the formation of 'hot tumor': increasing the numbers of tumor infiltrating lymphocytes and dendritic cells, elevating the ratio of M1/M2, and enhancing cytokine production in T cells. This normalized tumor immune microenvironment and enhanced anti-tumor immune response might contribute to the robust anti-tumor effect of YM101.

Conclusion: Our results demonstrated that YM101 could simultaneously block TGF-β and PD-L1 pathways and had a superior anti-tumor effect compared to the monotherapies.
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http://dx.doi.org/10.1186/s13045-021-01045-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7885589PMC
February 2021

Efficacy and safety of different dosages of rituximab for refractory generalized AChR myasthenia gravis: A meta-analysis.

J Clin Neurosci 2021 Mar 2;85:6-12. Epub 2021 Jan 2.

Department of Neurology, Tianjin Neurological Institute, Tianjin Medical University General Hospital, Tianjin 300052, China. Electronic address:

Background: Rituximab (RTX) is a mouse-human chimeric anti-CD20 monoclonal antibody and has been increasingly used for preventing relapses in myasthenia gravis (MG). However, the appropriate dose for maximizing the beneficial effects in refractory MG with acetylcholine receptor (AChR) autoantibody is a long-standing and critical debating question.

Methods: We performed a meta-analysis to evaluate the efficacy and safety of the different doses of RTX in 260 refractory AChR-MG patients.

Results: The AChR-MG patients were divided into low or routine RTX dose groups. An overall proportion of 77% (p = 0.000) AChR-MG patients demonstrated improved clinical status as indicated by the Myasthenia Gravis Foundation of America post-intervention scale (MGFA-PIS). There were 77.1% patients showed improved clinical status in lower dose of RTX group (p = 0.000) and 76.8% in routine protocol group (p = 0.000). Although we found there was no significant difference in the proportion of AChR-MG patients with improved clinical status or adverse reactions between the two groups, adverse reactions might be lower in the lower dose RTX group.

Conclusion: Most of refractory MG patients with anti-AChR autoantibody were well responsive and tolerated to RTX treatment. Repeated application of lower dose of RTX was effective and might be more appropriate for refractory AChR-MG patients with potential lower side effects.
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http://dx.doi.org/10.1016/j.jocn.2020.11.043DOI Listing
March 2021
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