Publications by authors named "Ming Xu"

1,397 Publications

  • Page 1 of 1

Exploration of the Hsa-miR-1587-Protein Interaction and the Inhibition to CASK.

Int J Mol Sci 2021 Oct 3;22(19). Epub 2021 Oct 3.

Beijing National Laboratory for Molecular Sciences, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.

Hsa-miR-1587 has been found to be capable of forming G-quadruplex structures and is overexpressed in multiple cancer cell lines. Here, we explored the interactions between miR-1587 and proteins. HuProt™ human proteome microarray was utilized to screen the binding proteins, and it was discovered that CASK could bind to miR-1587 on the base of the G-quadruplex structure. Moreover, reelin and p21, which are downstream of CASK, were downregulated both transcriptionally and translationally by miR-1587, uncovered by q-RT-PCR and Western blot assays. Bioinformatic analysis was performed on STRING and Panther platforms, leading to the discovery that miR-1587 may be involved in intracellular metabolic and transcriptional physiological processes. This study explores the interaction of hsa-miR-1587 with proteins and provides a new strategy for the regulation of G-rich microRNA's function.
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http://dx.doi.org/10.3390/ijms221910716DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8509502PMC
October 2021

[Discussions on Clinical Characteristics and Diagnostic Criteria of Pertussis in Children].

Sichuan Da Xue Xue Bao Yi Xue Ban 2021 Sep;52(5):839-843

Department of Pediatric Pulmonology, the Second Affiliated Hospital & Yuying Children's Hospital, Wenzhou Medical University, Wenzhou 325027, China.

Objective: To compare the clinical manifestations of pertussis in children of different ages and different immunization statuses in Wenzhou, and to explore the limitations of diagnostic criteria for pertussis.

Methods: The clinical data of 288 children diagnosed with pertussis at Yuying Children's Hospital & the Second Affiliated Hospital of Wenzhou Medical University from October 2017 to December 2019 were retrospectively analyzed. The clinical characteristics of children of different ages and different immunization statuses were analyzed. Their clinical data were compared to relevant diagnostic criteria of pertussis in children of different ages according to the and the diagnosis conformity rate was analyzed.

Results: Among the 288 children, 124 cases (43.06%) were 3 months old or younger, and 164 cases (288, 56.94%) were >3 months old. Among patients≤3 months of age, cyanosis, three-depression sign, face redness, dyspnea and peripheral blood lymphocyte ratio were significantly higher than those of patients >3 months of age. They also had higher incidence of pneumonia, higher proportion of developing severe pertussis, and longer stay at the hospital. All these findings showed statistically significant difference ( <0.05). 83 children were fully immunized (receiving the full course of vaccination), and 205 were not fully immunized (not receiving the full course of vaccination or being unvaccinated). The proportion of children presenting cyanosis, shortness of breath, three depression sign and face redness in the incomplete immunization group was higher than that in the complete immunization group. In the incomplete immunization group, the proportion of lymphocytes was higher, the level of C-reactive protein (CRP) was lower, and the length of hospitalization was longer than those of the complete immunization group. All the differences were statistically significant ( <0.05). Among patients aged ≤3 months, the conformity rate of diagnosis (112/114, 90.32%) upon admission was higher than that among patients aged >3 months (119/164, 72.56%). Among patients aged ≤3 months, 41.94% (52/124, while 54.03% (67/124) of the patients aged ≤3 months had WBC count <20×10 L .

Conclusion: Pertussis in children ≤3 months of age in Wenzhou City were more serious, showing higher rate of diagnosis conforming to the recommended clinical diagnostic criteria than that in children >3 months old. The WBC threshold in routine blood test of ≤3 months old could be lowered appropriately and the current diagnostic criteria still needed improvement.
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http://dx.doi.org/10.12182/20210960104DOI Listing
September 2021

A comparative study of asleep and awake deep brain stimulation robot-assisted surgery for Parkinson's disease.

NPJ Parkinsons Dis 2020 Oct 5;6(1):27. Epub 2020 Oct 5.

Department of Neurosurgery, The General Hospital of Northern Theater Command, 83 Wenhua Road, 110016, Shenyang, China.

To compare the differences between asleep and awake robot-assisted deep brain stimulation (DBS) surgery for Parkinson's Disease (PD), we conducted this retrospective cohort study included 153 PD patients undergoing bilateral robot-assisted DBS from June 2017 to August 2019, of which 58 cases were performed under general anesthesia (GA) and 95 cases under local anesthesia (LA). Procedure duration, stimulation parameters, electrode implantation accuracy, intracranial air, intraoperative electrophysiological signal length, complications, and Unified PD Rating Scale (UPDRS) measurements were recorded and compared. The clinical evaluation was conducted by two raters who were blinded to the choice of anesthesia. Procedure duration was significantly shorter in the GA group, while on stimulation off medication motor scores (UPDRS-III) were significantly improved in both the GA and LA group. ANCOVA covariated for the baseline UPDRS-III and levodopa challenge exhibited no significant differences. In terms of amplitude, frequency, and pulse width, the stimulation parameters used for DBS power-on were similar. There were no significant differences in electrode implantation accuracy, intraoperative electrophysiological signal length, or intracerebral hemorrhage (no occurrences in either group). The pneumocephalus volume was significantly smaller in the GA group. Six patients exhibited transient throat discomfort associated with tracheal intubation in the GA group. The occurrence of surgical incision infection was similar in both groups. Compared with the awake group, the asleep group exhibited a shorter procedure duration with a similar electrode implantation accuracy and short-term motor improvement. Robot-assisted asleep DBS surgery is a promising surgical method for PD.
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http://dx.doi.org/10.1038/s41531-020-00130-1DOI Listing
October 2020

Chinese Medicine Formula Huashibaidu Granule Early Treatment for Mild COVID-19 Patients: An Unblinded, Cluster-Randomized Clinical Trial.

Front Med (Lausanne) 2021 16;8:696976. Epub 2021 Sep 16.

Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, China.

Previous research suggested that Chinese Medicine (CM) Formula Huashibaidu granule might shorten the disease course in coronavirus disease 2019 (COVID-19) patients. This research aimed to investigate the early treatment effect of Huashibaidu granule in well-managed patients with mild COVID-19. An unblinded cluster-randomized clinical trial was conducted at the Dongxihu FangCang hospital. Two cabins were randomly allocated to a CM or control group, with 204 mild COVID-19 participants in each cabin. All participants received conventional treatment over a 7 day period, while the ones in CM group were additionally given Huashibaidu granule 10 g twice daily. Participants were followed up to their clinical endpoint. The primary outcome was worsening symptoms before the clinical endpoint. The secondary outcomes were cure and discharge before the clinical endpoint and alleviation of composite symptoms after the 7 days of treatment. All 408 participants were followed up to their clinical endpoint and included in statistical analysis. Baseline characteristics were comparable between the two groups ( > 0.05). The number of worsening patients in the CM group was 5 (2.5%), and that in the control group was 16 (7.8%) with a significant difference between groups ( = 0.014). Eight foreseeable mild adverse events occurred without statistical difference between groups ( = 0.151). Seven days of early treatment with Huashibaidu granule reduced the likelihood of worsening symptoms in patients with mild COVID-19. Our study supports Huashibaidu granule as an active option for early treatment of mild COVID-19 in similar well-managed medical environments. www.chictr.org.cn/showproj.aspx?proj=49408, identifier: ChiCTR2000029763.
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http://dx.doi.org/10.3389/fmed.2021.696976DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8481869PMC
September 2021

The ineffective emotion regulation strategies of heroin use disorder patients: An event-related potential study.

Drug Alcohol Depend 2021 Sep 24;228:109076. Epub 2021 Sep 24.

Lanzhou Drug Rehabilitation Hospital, Lanzhou, China.

Background: People with substance use disorders (SUDs) usually experience emotion dysregulation, which may be a consequence of or a risk factor for the development and maintenance of substance misuse. Despite growing evidence on emotion dysregulation among people with SUDs, relatively few studies have explored emotion dysregulation in heroin use disorder (HUD) patients.

Methods: Using event-related potentials (ERP), we compared the emotion regulation ability of 33 HUD patients and 30 healthy controls according to their average electroencephalogram amplitudes of the late positive potential (LPP) component in 400-1000 ms and 1000-2000 ms time windows, while viewing neutral and unpleasant emotional pictures, and using emotion regulation strategies (expressive suppression, cognitive reappraisal, and a combination) while viewing unpleasant pictures. We recorded their mood states and how successfully they used emotion regulation strategies in each block using 7-point scales.

Results: Relative to healthy controls, the LPP amplitudes of HUD patients were significantly lower when viewing emotional stimuli (p < 0.05) and using emotion regulation strategies (all p < 0.05). The left hemisphere was more active in healthy controls (p < 0.05, p < 0.01); there were no differences in scalp position activation among HUD patients.

Discussion: Compared to healthy controls, HUD patients' emotional arousal and emotion regulation ability were impaired, as reflected by the LPP component. Their abnormal scalp activation pattern may imply abnormal brain activity. Future research could explore this with electroencephalogram source analysis techniques, functional magnetic resonance imaging, or other technologies. Intervention effects for emotion dysregulation in HUD treatment are also worth exploring.
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http://dx.doi.org/10.1016/j.drugalcdep.2021.109076DOI Listing
September 2021

Superconducting Phase Induced by a Local Structure Transition in Amorphous Sb_{2}Se_{3} under High Pressure.

Phys Rev Lett 2021 Sep;127(12):127002

Center for High Pressure Science and Technology Advanced Research (HPSTAR), Shanghai 201203, People's Republic of China.

Superconductivity and Anderson localization represent two extreme cases of electronic behavior in solids. Surprisingly, these two competing scenarios can occur in the same quantum system, e.g., in an amorphous superconductor. Although the disorder-driven quantum phase transition has attracted much attention, its structural origins remain elusive. Here, we discovered an unambiguous correlation between superconductivity and density in amorphous Sb_{2}Se_{3} at high pressure. Superconductivity first emerges in the high-density amorphous (HDA) phase at about 24 GPa, where the density of glass unexpectedly exceeds its crystalline counterpart, and then shows an enhanced critical temperature when pressure induces crystallization at 51 GPa. Ab initio simulations reveal that the bcc-like local geometry motifs form in the HDA phase, arising from distinct "metavalent bonds." Our results demonstrate that HDA phase is critical for the incipient superconductive behavior.
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http://dx.doi.org/10.1103/PhysRevLett.127.127002DOI Listing
September 2021

Chinese environmentally extended input-output database for 2017 and 2018.

Sci Data 2021 Sep 30;8(1):256. Epub 2021 Sep 30.

Research Center for Central China Economic and Social Development, Nanchang University, Nanchang, 330031, China.

Environmental footprint analyses for China have gained sustained attention in the literature, which rely on quality EEIO databases based on benchmark input-output (IO) tables. The Chinese environmentally extended input-output (CEEIO) database series provide publically available EEIO databases for China for 1992, 1997, 2002, 2007, and 2012 with consistent and transparent data sources and database structure. Based on the latest benchmark IO tables for China for 2017 and 2018, here we develop the corresponding 2017 and 2018 CEEIO databases following the same method used to develop previous CEEIO databases. The 2017 and 2018 CEEIO databases cover 44 and 28 types of environmental pressures, respectively, and consider multiple sector classifications including ones consistent with previous CEEIO databases and ones following the 2017 China's national economy industry classification standard. A notable improvement in the 2017 and 2018 CEEIO databases is the comprehensive inclusion of CO emissions from additional industrial processes. This work provides a consistent update of the CEEIO database and enables a wide range of timely environmental footprint analyses related to China.
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http://dx.doi.org/10.1038/s41597-021-01035-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8484342PMC
September 2021

Frequent germplasm exchanges drive the high genetic diversity of Chinese-cultivated common apricot germplasm.

Hortic Res 2021 Oct 1;8(1):215. Epub 2021 Oct 1.

Liaoning Institute of Pomology, Yingkou, 115009, China.

The genetic diversity of germplasm is critical for exploring genetic and phenotypic resources and has important implications for crop-breeding sustainability and improvement. However, little is known about the factors that shape and maintain genetic diversity. Here, we assembled a high-quality chromosome-level reference of the Chinese common apricot 'Yinxiangbai', and we resequenced 180 apricot accessions that cover four major ecogeographical groups in China and other accessions from occidental countries. We concluded that Chinese-cultivated common apricot germplasms possessed much higher genetic diversity than those cultivated in Western countries. We also detected seven migration events among different apricot groups, where 27% of the genome was identified as being introgressed. Remarkably, we demonstrated that these introgressed regions drove the current high level of germplasm diversity in Chinese-cultivated common apricots by introducing different genes related to distinct phenotypes from different cultivated groups. Our results highlight the consideration that introgressed regions may provide an important reservoir of genetic resources that can be used to sustain modern breeding programs.
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http://dx.doi.org/10.1038/s41438-021-00650-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8484454PMC
October 2021

miR-497 defect contributes to gastric cancer tumorigenesis and progression via regulating CDC42/ITGB1/FAK/PXN/AKT signaling.

Mol Ther Nucleic Acids 2021 Sep 8;25:567-577. Epub 2021 Aug 8.

Department of Gastroenterology, Renmin Hospital of Wuhan University, 238 Jiefang Road, Wuchang, Wuhan, Hubei 430060, PR China.

Gastric cancer (GC) is one of the leading causes of cancer-related death worldwide. MicroRNAs (miRNAs) are known to be important regulators of GC. This study aims to investigate the role of miRNA (miR)-497 in GC. We demonstrated that the expression of miR-497 was downregulated in human GC tissues. After N-methyl-N-nitrosourea treatment, the incidence of GC in miR-497 knockout mice was significantly higher than that in wild-type mice. miR-497 overexpression suppressed GC cell proliferation, cell-cycle progression, colony formation, anti-apoptosis ability, and cell migration and invasion capacity. Additionally, miR-497 overexpression decreased the expression levels of cell division cycle 42 (CDC42) and integrin β1 (ITGB1) and inhibited the phosphorylation of focal adhesion kinase (FAK), paxillin (PXN), and serine-threonine protein kinase (AKT). Furthermore, overexpression of miR-497 inhibited the metastasis of GC cells , which could be counteracted by CDC42 restoration. Furthermore, the focal adhesion of GC cells was found to be regulated by miR-497/CDC42 axis via ITGB1/FAK/PXN/AKT signaling. Collectively, it is concluded that miR-497 plays an important role in the repression of GC tumorigenesis and progression, partly via the CDC42/ITGB1/FAK/PXN/AKT pathway.
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http://dx.doi.org/10.1016/j.omtn.2021.07.025DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8463315PMC
September 2021

Versatile Polymer Nanocapsules via Redox Competition.

Angew Chem Int Ed Engl 2021 Sep 27. Epub 2021 Sep 27.

University of California San Diego, Bioengineering, UNITED STATES.

Polymer nanocapsules have demonstrated significant value in materials science and biomedical technology, but require complicated and time-consuming synthetic steps. We report here the facile synthesis of monodisperse polymer nanocapsules via a redox-mediated kinetic strategy from two simple molecules: dopamine and benzene-1,4-dithiol (BDT). Specifically, BDT forms core templates and modulates the oxidation kinetics of dopamine into polydopamine (PDA) shells. These uniform nanoparticles can be tuned between ~70 and 200 nm because the core diameter directly depends on BDT while the shell thickness depends on dopamine. The supramolecular core can then rapidly disassemble in organic solvents to produce PDA nanocapsules. Such nanocapsules exhibit enhanced physicochemical performance (e.g., loading capacity, photothermal transduction, and anti-oxidation) versus their solid counterparts. Particularly, this method enables a straightforward encapsulation of functional nanoparticles providing opportunities for designing complex nanostructures such as yolk-shell nanoparticles.
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http://dx.doi.org/10.1002/anie.202110829DOI Listing
September 2021

The response of sediment microbial communities to temporal and site-specific variations of pollution in interconnected aquaculture pond and ditch systems.

Sci Total Environ 2021 Sep 21;806(Pt 1):150498. Epub 2021 Sep 21.

Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, Ministry of Education, College of Environment, Hohai University, Nanjing 210098, China; College of Environment, Hohai University, Nanjing 210098, China.

Sediment microbial communities play critical roles in the health of fish and the biogeochemical cycling of elements in aquaculture ecosystems. However, the response of microbial communities to temporal and spatial variations in interconnected aquaculture pond and ditch systems remains unclear. In this study, 61 sediment bacterial samples were collected over one year from 11 sites (including five ponds and six ditches) in a 30-year-old fish aquaculture farm. The 16S rRNA approach was used to determine the relative abundances of microbial communities in the sediment samples. The relationships among nutrients, heavy metals, and abundant microorganisms were analyzed. Our results showed that Proteobacteria, Bacteroides and Chloroflexi were the predominant phyla in the sediments of aquaculture pond, with average abundances of 36.33%, 18.60%, and 14.58%, respectively. The microbial diversity in aquaculture sediments was negatively correlated (P < 0.05) with the concentrations of total nitrogen and total phosphorus in sediments, indicating that the microbial diversity is highly associated with the remediation of nutrients in sediments. The sediment samples with high similarities were discovered by the t-distributed stochastic neighbor embedding (t-SNE) method. The site-specific correlations between specific microorganisms and heavy metals were explored. The network analysis revealed that the microbial diversities in aquaculture ponds were more stable than that in aquaculture ditches. The network analysis also illustrated that the microbial genera with low relative abundances may become key groups of microbial communities in sediment ecosystems. Our work deepens the understanding of the relationships between microbial communities and the spatiotemporal characteristics of surface water and sediments in aquaculture farms.
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http://dx.doi.org/10.1016/j.scitotenv.2021.150498DOI Listing
September 2021

[Effect of electroacupuncture on urodynamics of neurogenic bladder and PACAP/cAMP/PKA signaling pathway in detrusor tissue of rats after suprasacral spinal cord injury].

Zhen Ci Yan Jiu 2021 Sep;46(9):728-34

College of Acupuncture and Massage, Hunan University of Chinese Medicine, Changsha 410208, China.

Objective: To observe the effect of electroacupuncture (EA) on urodynamics of neurogenic bladder and pituitary adenylate cyclase activating peptide(PACAP)/cyclic adenosine monophosphate (cAMP)/protein kinase A(PKA) signaling pathway in detrusor tissue of rats after suprasacral spinal cord injury (SCI), so as to explore its possible mechanism in improving detrusor hyperreflexia bladder function after shock stage of suprasacral SCI.

Methods: Female SD rats were randomly divided into control, sham operation, model and EA groups, with 12 rats in each group. T10 spinal cord transection (SCT) was performed by surgery. Rats in the EA group were given EA (10 Hz/50 Hz, 20 min) at "Ciliao" (BL32), "Zhongji" (CV3) and "Sanyinjiao" (SP6) once daily for 7 days. After the intervention, urodynamics testing was detected in each group. HE staining was used to observe the morphological changes of bladder detrusor. The protein and mRNA expression of PACAP38 in detrusor was detected by immunohistochemistry, Western blot and real-time quantitive PCR, respectively. The contents of cAMP and PKA were determined by ELISA.

Results: Compared with the control and sham operation groups, the maximum bladder capacity and bladder com-pliance, and the protein and mRNA expression of PACAP38, and the contents of cAMP and PKA of the model group were significantly decreased (<0.01, <0.05), while the base pressure and leakage point pressure of bladder were significantly increased (<0.01). After EA intervention, the above indexes were all reversed in the EA group relevant to those of the model group (<0.01, <0.05).

Conclusion: EA at BL32, CV3 and SP6 can improve the bladder function in rats with bladder detrusor hyperreflexia after SCI, which may be related to its effect in activating the PACAP/cAMP/PKA signaling pathway in detrusor tissue.
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http://dx.doi.org/10.13702/j.1000-0607.200880DOI Listing
September 2021

Quantum Yield Measurements of Photochemical Reaction-Based Afterglow Luminescence Materials.

J Phys Chem Lett 2021 Oct 24;12(39):9455-9462. Epub 2021 Sep 24.

Department of Chemistry & State Key Laboratory of Molecular Engineering of Polymers, Fudan University, Shanghai 200433, China.

Afterglow materials have become one of the most promising luminescent materials due to their long luminescence lifetime. Photoluminescence quantum yield (PLQY), as one of the most fundamental and essential parameters of luminescent materials, can directly evaluate the luminescence properties of emissive compounds. Recently, a type of afterglow material based on a photochemical reaction has been developed. However, there is no suitable method to measure the PLQY of these afterglow materials due to their special luminescence principle. Herein, we present a method to measure the PLQY for these afterglow materials by collecting the luminescent dynamic curves of emission and excitation light at specific wavelength regions using a commercial spectrometer and an integrating sphere. We found that the emitted photons of this kind of afterglow material are in direct proportion to the excitation time, which means the PLQY is irrelevant to the excitation time.
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http://dx.doi.org/10.1021/acs.jpclett.1c02715DOI Listing
October 2021

Preoperative Survival Prediction in Intrahepatic Cholangiocarcinoma Using a Ultrasound-Based Radiographic-Radiomics Signature.

J Ultrasound Med 2021 Sep 21. Epub 2021 Sep 21.

Department of Medical Ultrasonics, Institute of Diagnostic and Interventional Ultrasound, Ultrasomics Artificial Intelligence X-Lab, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China.

Objectives: To construct a preoperative model for survival prediction in intrahepatic cholangiocarcinoma (ICC) patients using ultrasound (US) based radiographic-radiomics signatures.

Methods: Between April 2010 and September 2015, 170 patients with ICC who underwent curative resection were retrospectively recruited. Overall survival (OS)-related radiographic signatures and radiomics signatures based on preoperative US were built and assessed through a time-dependent receiver operating characteristic curve analysis. A nomogram was developed based on the selected predictors from the radiographic-radiomics signatures and clinical and laboratory results of the training cohort (n = 127), validated in an independent testing cohort (n = 43) by the concordance index (C-index), and compared with the Tumor Node Metastasis (TNM) cancer staging system as well as the radiographic and radiomics nomograms.

Results: The median areas under the curve of the radiomics signature and radiographic signature were higher than that of the TNM staging system in the testing cohort, although the values were not significantly different (0.76-0.82 versus 0.62, P = .485 and .264). The preoperative nomogram with CA 19-9, sex, ascites, radiomics signature, and radiographic signature had C-indexes of 0.72 and 0.75 in the training and testing cohorts, respectively, and it had significantly higher predictive performance than the 8th TNM staging system in the testing cohort (C-index: 0.75 versus 0.67, P = .004) and a higher C-index than the radiomics nomograms (0.75 versus 0.68, P = .044).

Conclusions: The preoperative nomogram integrated with the radiographic-radiomics signature demonstrated good predictive performance for OS in ICC and was superior to the 8th TNM staging system.
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http://dx.doi.org/10.1002/jum.15833DOI Listing
September 2021

Real-time artificial intelligence for detecting focal lesions and diagnosing neoplasms of the stomach by white-light endoscopy (with videos).

Gastrointest Endosc 2021 Sep 18. Epub 2021 Sep 18.

Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan, China; Key Laboratory of Hubei Province for Digestive System Disease, Renmin Hospital of Wuhan University, Wuhan, China; Hubei Provincial Clinical Research Center for Digestive Disease Minimally Invasive Incision, Renmin Hospital of Wuhan University, Wuhan, China. Electronic address:

Background And Aims: White-light endoscopy (WLE) is the most pivotal tool to detect gastric cancer in the early stage. However, the skill among endoscopists varies greatly. Here, we aim to develop a deep learning-based system named ENDOANGEL-LD (lesion detection) to assist in detecting all focal gastric lesions and predicting neoplasms by WLE.

Methods: Endoscopic images were retrospectively obtained from Renmin Hospital of Wuhan University (RHWU) for the development, validation and internal test of the system. Additional external tests were conducted in 5 other hospitals to evaluate the robustness. Stored videos from RHWU were used for assessing and comparing the performance of ENDOANGEL-LD with that of experts. Prospective consecutive patients undergoing upper endoscopy were enrolled from May 6, 2021 to Aug 2 2021 in RHWU to assess clinical practice applicability.

Results: Over 10 thousand patients undergoing upper endoscopy were enrolled in this study. The sensitivities were 96.9% and 95.6% for detecting gastric lesions, 92.9% and 91.7% for diagnosing neoplasm in internal and external patients, respectively. In 100 videos, ENDOANGEL-LD achieved superior sensitivity and negative predictive value for detecting gastric neoplasm than that of experts (100% vs 85.5%±3.4%, p=0.003; 100% vs 86.4%±2.8%, p=0.002). In 2010 prospective consecutive patients, ENDOANGEL-LD achieved a sensitivity of 92.8% for detecting gastric lesions with 3.04±3.04 false positives per gastroscopy, and a sensitivity of 91.8% and specificity of 92.4% for diagnosing neoplasm.

Conclusions: The results show that ENDOANGEL-LD has great potential for assisting endoscopists in screening gastric lesions and suspicious neoplasms in clinical work.
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http://dx.doi.org/10.1016/j.gie.2021.09.017DOI Listing
September 2021

A critical review of the environmental impacts of manufactured nano-objects on earthworm species.

Environ Pollut 2021 Aug 31;290:118041. Epub 2021 Aug 31.

Beijing Key Laboratory of Farmland Soil Pollution Prevention and Remediation and College of Resources and Environmental Sciences, China Agricultural University, Beijing, 100193, PR China. Electronic address:

The presence of manufactured nano-objects (MNOs) in various consumer or their (future large-scale) use as nanoagrochemical have increased with the rapid development of nanotechnology and therefore, concerns associated with its possible ecotoxicological effects are also arising. MNOs are releasing along the product life cycle, consequently accumulating in soils and other environmental matrices, and potentially leading to adverse effects on soil biota and their associated processes. Earthworms, of the group of Oligochaetes, are an ecologically significant group of organisms and play an important role in soil remediation, as well as acting as a potential vector for trophic transfer of MNOs through the food chain. This review presents a comprehensive and critical overview of toxic effects of MNOs on earthworms in soil system. We reviewed pathways of MNOs in agriculture soil environment with its expected production, release, and bioaccumulation. Furthermore, we thoroughly examined scientific literature from last ten years and critically evaluated the potential ecotoxicity of 16 different metal oxide or carbon-based MNO types. Various adverse effects on the different earthworm life stages have been reported, including reduction in growth rate, changes in biochemical and molecular markers, reproduction and survival rate. Importantly, this literature review reveals the scarcity of long-term toxicological data needed to actually characterize MNOs risks, as well as an understanding of mechanisms causing toxicity to earthworm species. This review sheds light on this knowledge gap as investigating bio-nano interplay in soil environment improves our major understanding for safer applications of MNOs in the agriculture environment.
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http://dx.doi.org/10.1016/j.envpol.2021.118041DOI Listing
August 2021

LINC00941 promotes glycolysis in pancreatic cancer by modulating the Hippo pathway.

Mol Ther Nucleic Acids 2021 Dec 16;26:280-294. Epub 2021 Jul 16.

Department of Hepatopancreatobiliary Surgery, Translational Medical Center for Stem Cell Therapy, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200123, China.

Pancreatic ductal adenocarcinoma (PDAC) is one of most lethal cancers and is projected to be the second leading cause of cancer deaths in the United States by 2030. The lack of effective treatment and increased incidence in PDAC encourage a deeper knowledge of PDAC progression. By analyzing a long noncoding RNA (lncRNA) dataset, we found that increased LINC00941 expression led to poor outcomes in PDAC patients. Furthermore, and experiments revealed that LINC00941 promoted PDAC cancer cell growth by enhancing aerobic glycolysis. Mechanistically, LINC00941 was found to interact with mammalian STE20-like protein kinase 1 (MST1), which facilitated the protein phosphatase 2A (PP2A)-mediated dephosphorylation of MST1, resulting in Hippo pathway activation and consequently, enhanced glycolysis in PDAC. These results suggest that LINC00941 plays a key role in regulating PDAC tumorigenesis, potentially highlighting novel avenues for PDAC therapy.
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http://dx.doi.org/10.1016/j.omtn.2021.07.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8413680PMC
December 2021

UHPLC-MS/MS analysis of cAMP and cGMP in rat plasma as potential biomarkers of Yin-Yang disharmony in traditional Chinese medicine.

J Pharm Anal 2021 Aug 5;11(4):458-464. Epub 2020 Sep 5.

Laboratory of Drug Metabolism and Pharmacokinetics, School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, 110016, PR China.

Cyclic 3',5'-adenosine monophosphate (cAMP) and cyclic 3',5'-guanosine monophosphate (cGMP) are considered as potential biomarkers for Yin-Yang disharmony in traditional Chinese medicine. However, phosphodiesterase-mediated ex vivo degradation of these molecules in biological samples may result in their underestimation. In the present study, a ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was developed for determination of cAMP and cGMP in rat plasma, with special consideration of their stability ex vivo. Following precipitation of proteins from plasma samples with 0.4 M perchloric acid, the analytes were chromatographed on a Shimadzu Shim-pack-XR-ODS II column with 2.5 mM ammonium acetate and methanol in gradient mode. The MS/MS detection was performed using multiple reaction monitoring in the positive electrospray ionization mode. The lower limit of quantification was 0.27 ng/mL for cAMP and 0.37 ng/mL for cGMP. The method was used to determine the plasma cAMP and cGMP levels in normal and Yin deficiency diabetic rats treated with or without . The developed method may be useful for evaluating the regulatory effects of Chinese herbal medicine on the levels of cAMP and cGMP in the body.
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http://dx.doi.org/10.1016/j.jpha.2020.09.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8424358PMC
August 2021

Efficacy and safety of dissimilar acupuncture intervention time-points in treating stroke: a systematic review and network meta-analysis.

Ann Palliat Med 2021 Sep 6. Epub 2021 Sep 6.

College of Acupuncture-Moxibustion and Tuina, Hunan University of Chinese Medicine, Changsha, China.

Background: Impairment of motor function and activities of daily living is very common in post-stroke patients. Acupuncture has been used in stroke for a long time. Although growing evidence supports the beneficial effects of acupuncture, there is still no clear conclusion on the optimal intervention time-point for acupuncture in stroke. Thus, we tried to assess the efficacy and safety of dissimilar acupuncture intervention time-points by network meta-analysis (NMA).

Methods: A retrieval was performed in several databases from beginning to October 2020. We only enrolled randomized controlled trials (RCTs) testing acupuncture for stroke, and the outcome indicators measured were Fugl-Meyer assessment (FMA) score, Barthel Index (BI), and adverse events.

Results: Thirty-eight trials involving 3,836 participants were included in this NMA, the vast majority of which had a low or unclear risk of selection bias, detection bias, reporting bias, attrition bias, but had a high risk of performance bias. The results showed as following: (I) acupuncture treatment (Acu) is significantly more effective than non-acupuncture treatment (Non-Acu) with a good safety. (II) In terms of FMA, acupuncture within 48 h post-stroke (Acu I) was superior to acupuncture within 2-15 d post-stroke (Acu II) [standard mean difference (SMD): 7.17; 95% confidence interval (CI), 1.11 to 13.22], acupuncture within 16-30 d post-stroke (Acu III) (SMD: 20.73; 95% CI, 13.68 to 27.78), acupuncture within 1 month to half a year post-stroke (Acu IV) (SMD: 26.95; 95% CI, 14.88 to 39.02). As for BI, Acu I was the optimal timepoint, comparing with Acu III (SMD: 15.18; 95% CI, 8.97 to 21.39) and Acu IV (SMD: 22.88; 95% CI, 11.07 to 34.69). (III) Results of ranking indicated that Acu was better than Non-Acu at a similar stage in improving FMA and BI, while Acu I was the optimal intervention time-point, followed by Acu II.

Conclusions: Although the credibility of our conclusions is low, our NMA indicates that acupuncture can be beneficial for stroke survivors with good safety. In terms of improvement in FMA score and BI, the soon use of acupuncture the better the efficacy. The optimal acupuncture intervention time-point for stroke is within 48 h post-stroke, and the significant validity period lasts until 15 days after onset.
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http://dx.doi.org/10.21037/apm-21-1127DOI Listing
September 2021

Association of postoperative delirium with cognitive outcomes: A meta-analysis.

J Clin Anesth 2021 Sep 2;75:110496. Epub 2021 Sep 2.

Department of Critical Care Medicine, Beijing Tiantan Hospital, Capital Medical University, Beijing, China. Electronic address:

Study Objective: To determine the association between postoperative delirium (POD) and cognitive outcomes at least 1 month after surgery in elderly patients, and synthesize the dynamic risk trajectory of cognition impairment after POD.

Design: Meta-analysis searching PubMed, Cochrane and EMBASE from inception to November 1, 2020. The terms postoperative delirium, delirium after surgery, postsurgical delirium, postoperative cogniti*, postoperative cognitive dysfunction, postoperative cognition decline, cognitive decline, cognitive impair* and dement* were searched alone or in combination.

Measurements: Inclusion criteria were prospective cohort studies investigating the association between POD and cognitive outcomes in patients aged ≥60 years underwent surgery. The primary outcome was the association between POD and cognitive outcomes at 1 or more months after surgery. We considered cognitive outcomes measured up to 12 months after surgery as short-term and beyond 12 months as long-term. Two authors performed the study screening, data extraction and quality assessments. Effect sizes were calculated as Hedges g or Odds ratio (OR) based on random- and fixed-effects models. Meta-regression was conducted to analyze the role of potential contributors to heterogeneity.

Main Results: Eighteen studies were included. Our result showed a significant and medium association between POD and cognitive outcomes after at least 1 month postoperatively (g = 0.61 95% CI 0.43-0.79; I = 65.1%), indicating that patients with POD were associated with worse cognitive outcomes. The association of POD with short- and long-term cognitive impairment were also both significant (short-term: g = 0.46 95% CI 0.24-0.68; I = 53.1%; and long-term: g = 0.82 95% CI 0.57-1.06; I = 57.1%). A multivariate meta-regression suggested that age and measure of delirium were significant sources of heterogeneity. POD was also associated with the significant risk for dementia (OR = 6.08 95% CI 3.80-9.72; I = 0) as well as attention (OR = 1.74 95% CI 1.13-2.68; I = 0), executive (OR = 1.33 95% CI 1.00-1.80; I = 0) and memory impairment (OR = 1.59 95% CI 1.20-2.10; I = 43.0%). Additionally, our results showed that the risk trajectory for cognitive decline associated with POD within five years after surgery revealed exponential growth.

Conclusions: This is the first meta-analysis quantifying the association between POD and cognitive outcomes. Our results showed that POD was significantly associated with worse cognitive outcomes, including short- and long-term cognitive outcomes following surgery.
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http://dx.doi.org/10.1016/j.jclinane.2021.110496DOI Listing
September 2021

Visualization of Bubble Nucleation and Growth Confined in 2D Flakes.

Small 2021 Oct 2;17(39):e2103301. Epub 2021 Sep 2.

School of Materials Science and Engineering, Jiangxi Engineering Laboratory for Advanced Functional Thin Films, Jiangxi Key Laboratory for Two-Dimensional Materials and Jiangxi Key Laboratory for Multiscale Interdisciplinary Study, Nanchang University, Nanchang, Jiangxi, 330031, China.

The nucleation and growth of bubbles within a solid matrix is a ubiquitous phenomenon that affects many natural and synthetic processes. However, such a bubbling process is almost "invisible" to common characterization methods because it has an intrinsically multiphased nature and occurs on very short time/length scales. Using in situ transmission electron microscopy to explore the decomposition of a solid precursor that emits gaseous byproducts, the direct observation of a complete nanoscale bubbling process confined in ultrathin 2D flakes is presented here. This result suggests a three-step pathway for bubble formation in the confined environment: void formation via spinodal decomposition, bubble nucleation from the spherization of voids, and bubble growth by coalescence. Furthermore, the systematic kinetics analysis based on COMSOL simulations shows that bubble growth is actually achieved by developing metastable or unstable necks between neighboring bubbles before coalescing into one. This thorough understanding of the bubbling mechanism in a confined geometry has implications for refining modern nucleation theories and controlling bubble-related processes in the fabrication of advanced materials (i.e., topological porous materials).
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http://dx.doi.org/10.1002/smll.202103301DOI Listing
October 2021

Trade-related water scarcity risk under the Belt and Road Initiative.

Sci Total Environ 2021 Aug 20;801:149781. Epub 2021 Aug 20.

School of Management and Economics, Beijing Institute of Technology, Beijing 100081, People's Republic of China; Center for Energy and Environmental Policy Research, Beijing Institute of Technology, Beijing 100081, People's Republic of China. Electronic address:

Increasing trade cooperation under the Belt and Road (B&R) Initiative has promoted economic development and intensified the water scarcity risk transmission between China and countries along the route (B&R countries). Local water scarcity risk (LWSR, the potential direct production losses induced by local water scarcity) can transcend geographical boundaries through global supply chains and influence production activities in downstream economies. To understand the vulnerability of the Initiative to water scarcity, we investigated the impacts of LWSR in China and B&R countries on each other's economies during 2001-2013, using a global environmentally extended multi-regional input-output model. Results reveal that more than 80% of China's trade-related water scarcity risk imports (TWSR imports, the vulnerability to foreign water scarcity risk through imports) originates from B&R countries. The share of TWSR from China in total imports of B&R countries has steadily increased. In particular, India, Thailand, Iran, Pakistan and Kazakhstan have the largest TWSR exports (LWSR in each nation transmitted to other nations through its exports) to China, while South Korea, Thailand, Malaysia, Singapore and Indonesia have the largest imports from China. Water scarcity to their Agriculture sectors is responsible for TWSR transmission between them. Our study can contribute to the policy-making of governments and firms involved in mitigating the supply chain wide water scarcity risk. It also reveals the need for nations to collectively manage water resources to achieve sustainable development.
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http://dx.doi.org/10.1016/j.scitotenv.2021.149781DOI Listing
August 2021

Electroencephalogram Application for the Analysis of Stress Relief in the Seasonal Landscape.

Authors:
Yuting Wang Ming Xu

Int J Environ Res Public Health 2021 08 12;18(16). Epub 2021 Aug 12.

Henan Key Laboratory of Earth System Observation and Modeling, Kaifeng 475004, China.

This study proposes an integrated approach to assess the psychological and physiological responses of people in natural seasonal landscapes. The questionnaire of restoration outcomes scale (ROS), willingness to visit (WTV), cultural ecosystem services (CES) cognitive classification, and the neuroscientific technique based on electroencephalogram (EEG) measurements were applied. The effects of different landscapes on human perception were studied by comparing the EEG data of different landscape types and different seasons. The coupling relationship between EEG data and stress recovery was also examined. The results showed the following: First, there was a significant difference between the winter landscape and the summer natural landscape. Second, only the winter landscape showed significant gender differences. Third, the values of ROS and WTV in the summer landscape were greater than those in the winter landscape. Fourth, the number of CES in the summer landscape was significantly higher than that in the winter landscape, and the number of CES in water was higher than that in the forest and grassland. Thus, brain wave data and quantified values from questionnaires including ROS, WTV, and CES showed significant seasonality. Therefore, an EEG can be used as a new, more objective tool and method for landscape evaluation and planning in the future.
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http://dx.doi.org/10.3390/ijerph18168522DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8395011PMC
August 2021

Combination of Hua Shi Bai Du granule (Q-14) and standard care in the treatment of patients with coronavirus disease 2019 (COVID-19): A single-center, open-label, randomized controlled trial.

Phytomedicine 2021 Oct 17;91:153671. Epub 2021 Jul 17.

China Academy of Chinese Medical Sciences Guanganmen Hospital, Beijing, China.

Objective: To evaluate the efficacy and safety of Hua Shi Bai Du Granule (Q-14) plus standard care compared with standard care alone in adults with coronavirus disease (COVID-19).

Study Design: A single-center, open-label, randomized controlled trial.

Setting: Wuhan Jinyintan Hospital, Wuhan, China, February 27 to March 27, 2020.

Participants: A total of 204 patients with laboratory-confirmed COVID-19 were randomized into the treatment group and control group, consisting of 102 patients in each group.

Interventions: In the treatment group, Q-14 was administered at 10 g (granules) twice daily for 14 days, plus standard care. In the control group, patients were provided standard care alone for 14 days.

Main Outcome Measure: The primary outcome was the conversion time for the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral assay. Adverse events were analyzed in the safety population.

Results: Among the 204 patients, 195 were analyzed according to the intention-to-treat principle. A total of 149 patients (71 vs. 78 in the treatment and control groups, respectively) tested negative via the SARS-CoV-2 viral assay. There was no statistical significance in the conversion time between the treatment group and control group (Full analysis set: Median [interquartile range]: 10.00 [9.00-11.00] vs. 10.00 [9.00-11.00]; Mean rank: 67.92 vs. 81.44; P = 0.051). The recovery time for fever was shorter in the treatment group than in the control group. The disappearance rate of symptoms like cough, fatigue, and chest discomfort was significantly higher in the treatment group. In chest computed tomography (CT) examinations, the overall evaluation of chest CT examination after treatment compared with baseline showed that more patients improved in the treatment group. There were no significant differences in the other outcomes.

Conclusion: The combination of Q-14 and standard care for COVID-19 was useful for the improvement of symptoms (such as fever, cough, fatigue, and chest discomfort), but did not result in a significantly higher probability of negative conversion in the SARS-CoV-2 viral assay. No serious adverse events were observed.

Trial Registration: ChiCTR2000030288.
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http://dx.doi.org/10.1016/j.phymed.2021.153671DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8285932PMC
October 2021

MicroRNA-23a-5p Is Involved in the Regulation of Lipopolysaccharide-Induced Acute Lung Injury by Targeting HSP20/ASK1.

Oxid Med Cell Longev 2021 11;2021:9942557. Epub 2021 Aug 11.

Department of Thoracic Surgery, Zhongnan Hospital of Wuhan University, Wuhan, 430071 Hubei, China.

Inflammation and oxidative stress contribute to the progression of acute lung injury (ALI). MicroRNA-23a-5p (miR-23a-5p) has been reported to regulate inflammation and oxidative stress; however, its role in ALI is still poorly elucidated. Mice were intravenously treated with the miR-23a-5p antagomir, agomir, or the negative controls for 3 consecutive days and then received a single intratracheal injection of lipopolysaccharide (LPS, 5 mg/kg) to induce ALI. Pulmonary function, bronchoalveolar lavage fluids (BALFs), arterial blood gas, and molecular biomarkers associated with inflammation and oxidative stress were analyzed. In addition, murine peritoneal macrophages were isolated and treated with LPS to verify the role of miR-23a-5p in vitro. We detected an elevation of miR-23a-5p expression in the lungs from ALI mice. The miR-23a-5p antagomir was prevented, whereas the miR-23a-5p agomir aggravated inflammation, oxidative stress, lung tissue injury, and pulmonary dysfunction in LPS-treated mice. Besides, the miR-23a-5p antagomir also reduced the productions of proinflammatory cytokines and free radicals in LPS-treated primary macrophages, which were further augmented in cells following the miR-23a-5p agomir treatment. Additional findings demonstrated that the miR-23a-5p agomir exacerbated LPS-induced ALI via activating apoptosis signal-regulating kinase 1 (ASK1), and that pharmacological or genetic inhibition of ASK1 significantly repressed the deleterious effects of the miR-23a-5p agomir. Moreover, we proved that the miR-23a-5p agomir activated ASK1 via directly reducing heat shock protein 20 (HSP20) expression. miR-23a-5p is involved in the regulation of LPS-induced inflammation, oxidative stress, lung tissue injury, and pulmonary dysfunction by targeting HSP20/ASK1, and it is a valuable therapeutic candidate for the treatment of ALI.
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http://dx.doi.org/10.1155/2021/9942557DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8376430PMC
August 2021

Response of net reduction rate in vegetation carbon uptake to climate change across a unique gradient zone on the Tibetan Plateau.

Environ Res 2021 Aug 18;203:111894. Epub 2021 Aug 18.

Arid Land Research Center, Tottori University, Tottori, 6800001, Japan. Electronic address:

The Tibetan Plateau (TP) has a variety of vegetation types that range from alpine tundra to tropic evergreen forest, which play an important role in the global carbon (C) cycle and is extremely vulnerable to climate change. The vegetation C uptake is crucial to the ecosystem C sequestration. Moreover, net reduction in vegetation C uptake (NRVCU) will strongly affect the C balance of terrestrial ecosystem. Until now, there is limited knowledge on the recovery process of vegetation net C uptake and the spatial-temporal patterns of NRVCU after the disturbance that caused by climate change and human activities. Here, we used the MODIS-derived net primary production to characterize the spatial-temporal patterns of NRVCU. We further explored the influence factors of the net reduction rate in vegetation C uptake (NRRVCU) and recovery processes of vegetation net C uptake across a unique gradient zone on the TP. Results showed that the total net reduction amount of vegetation C uptake gradually decreased from 2000 to 2015 on the TP (Slope = -0.002, P < 0.05). Specifically, an increasing gradient zone of multi-year average of net reduction rate in vegetation carbon uptake (MYANRRVCU) from east to west was observed. In addition, we found that the recovery of vegetation net C uptake after the disturbance caused by climate change and anthropogenic disturbance in the gradient zone were primarily dominated by precipitation and temperature. The findings revealed that the effects of climate change on MYANRRVCU and vegetation net C uptake recovery differed significantly across geographical space and vegetation types. Our results highlight that the biogeographic characteristics of the TP should be considered for combating future climate change.
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http://dx.doi.org/10.1016/j.envres.2021.111894DOI Listing
August 2021

Electrocatalytic upcycling of polyethylene terephthalate to commodity chemicals and H fuel.

Nat Commun 2021 Aug 17;12(1):4679. Epub 2021 Aug 17.

Department of Chemistry, Tsinghua University, Beijing, China.

Plastic wastes represent a largely untapped resource for manufacturing chemicals and fuels, particularly considering their environmental and biological threats. Here we report electrocatalytic upcycling of polyethylene terephthalate (PET) plastic to valuable commodity chemicals (potassium diformate and terephthalic acid) and H fuel. Preliminary techno-economic analysis suggests the profitability of this process when the ethylene glycol (EG) component of PET is selectively electrooxidized to formate (>80% selectivity) at high current density (>100 mA cm). A nickel-modified cobalt phosphide (CoNiP) electrocatalyst is developed to achieve a current density of 500 mA cm at 1.8 V in a membrane-electrode assembly reactor with >80% of Faradaic efficiency and selectivity to formate. Detailed characterizations reveal the in-situ evolution of CoNiP catalyst into a low-crystalline metal oxy(hydroxide) as an active state during EG oxidation, which might be responsible for its advantageous performances. This work demonstrates a sustainable way to implement waste PET upcycling to value-added products.
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http://dx.doi.org/10.1038/s41467-021-25048-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371182PMC
August 2021

Roles and Mechanisms of TGR5 in the Modulation of CD4 T Cell Functions in Myocardial Infarction.

J Cardiovasc Transl Res 2021 Aug 16. Epub 2021 Aug 16.

Department of Cardiology and Institute of Vascular Medicine, Peking University Third Hospital, Beijing, 100191, China.

Bile acid receptor TGR5 has been proved to play protective roles in the process of myocardial infarction (MI). Recently, we found spleen weight of Tgr5 mice was increased at 7-day post-MI but not in Tgr5 mice. Since the spleen is one of the main resources of immune and inflammatory cells post-MI, we conducted flow cytometry analysis of multiple immune cells in the heart post-MI. It showed the recruitment of CD4 T cells and CD8 T cells was continuously more in the heart of Tgr5 mice post-MI until 7 days after MI. Furthermore, CD4-specific TGR5 depletion mice exhibited aggravated ischemic injury. The mRNA expressions of the markers of Th1 and Treg were upregulated in the heart of Tgr5 mice at 7-day post-MI. These results suggested TGR5 modulates CD4 T cell functions and subsets distribution in the heart, and plays protective roles in myocardial infarction.
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http://dx.doi.org/10.1007/s12265-021-10164-2DOI Listing
August 2021

CircTHBS1 facilitates the progression of interstitial cystitis depending on the regulation of miR-139-5p/MFN2 axis.

Drug Dev Res 2021 Aug 9. Epub 2021 Aug 9.

Department of Urology, The Second Affiliated Hospital of Soochow University, Suzhou, China.

Circular RNA (circRNA) have been found to play an important role in the progression of many diseases, including interstitial cystitis (IC). However, the role of circTHBS1 in IC progression is still unclear. Exploring the role and potential molecular mechanism of circTHBS1 in the development of IC. The enzyme-linked immunosorbent assay was used to assess the levels of inflammatory cytokines. The expression levels of circTHBS1, microRNA (miR)-139-5p, and mitofusin 2 (MFN2) were evaluated using quantitative real-time PCR. Cell proliferation and migration were determined using MTT assay, Edu staining, and transwell assay. The protein levels of epithelial-mesenchymal transition (EMT) markers and MFN2 were examined using western blot analysis. The relationship between miR-139-5p and circTHBS1 or MFN2 was confirmed using the dual-luciferase reporter assay and RIP assay. CircTHBS1 was highly repressed in IC tissues and cells, and its expression was positively correlated with the inflammatory response of IC patients. CircTHBS1 could promote the proliferation, migration, EMT process, and inflammation of IC cells, while its knockdown had an opposite effect. CircTHBS1 could serve as a sponge of miR-139-5p, and miR-139-5p could participate in the regulation of circTHBS1 on IC cell progression. In addition, miR-139-5p could target MFN2, and it could inhibit the progression of IC cells by targeting MFN2. Furthermore, circTHBS1 sponged miR-139-5p to positively regulate MFN2. CircTHBS1 promoted IC cell proliferation, migration, EMT process, and inflammation by regulating the miR-139-5p/MFN2 axis indicating that circTHBS1 might be a potential target for IC treatment.
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http://dx.doi.org/10.1002/ddr.21864DOI Listing
August 2021

How Can Agricultural Corporate Build Sustainable Competitive Advantage through Green Intellectual Capital? A New Environmental Management Approach to Green Agriculture.

Int J Environ Res Public Health 2021 07 26;18(15). Epub 2021 Jul 26.

Yatai School of Business Administration, Jilin University of Finance and Economics, 3699 Jingyue Street, Changchun 130117, China.

Based on natural resource-based theory, this study constructed a relational model between green intellectual capital, green innovation, and an agricultural corporate sustainable competitive advantage. The samples included a total of 341 agricultural companies in China, and multiple regression methods are used for the analysis. The results showed that green product innovation and green process innovation had a mediation effect between green human capital, green structural capital, green relational capital, and the sustainable competitive advantage of agricultural corporate. Beyond the simple moderation effect, a new integrated moderated-mediation effect model was established. It was shown that environmental leadership, green organizational identification, and green dynamic capability had different moderated-mediation effects under different conditions. The study is expected to close the previous research gaps and insufficiency in agricultural corporate environmental management and green agricultural. The empirical results and conclusions bring enlightenment and meaningful theoretical guidance to managers, researchers, practitioners, and policy makers in the green and sustainable development of agricultural corporates. The new environmental management path can help agricultural corporates conduct green innovation effectively, adapt to the green agricultural products market, and achieve sustainable competitive advantage. Ultimately, this will help to accelerate the development of green agriculture.
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http://dx.doi.org/10.3390/ijerph18157900DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8345645PMC
July 2021
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