Publications by authors named "Ming Lin"

367 Publications

Autologous decellularized extracellular matrix promotes adipogenic differentiation of adipose derived stem cells in low serum culture system by regulating the ERK1/2-PPARγ pathway.

Adipocyte 2021 Dec;10(1):174-188

Deprtment of Plastic Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou City, China.

High viability and further adipogenic differentiation of adipose-derived stem cells (ADSCs) are fundamental for engraftment and growth of the transplanted adipose tissue. It has been demonstrated that extracellular matrix (ECM) regulates cell proliferation and differentiation by interacting with ERK1/2 signalling pathway. In this study, we prepared autologous decellularized extracellular matrix (d-ECM) and explored its effect on the proliferation and adipogenic ability of ADSCs in low serum culture. We found that 2% foetal bovine serum (FBS) in growth medium inhibited cell viability and DNA replication, and decreased mRNA and protein levels of PPARγ and C/EPBα compared with 10% FBS. Correspondingly, after 14-days adipogenic induction, cells cultured in 2% FBS possessed lower efficiency of adipogenesis and expressed less adipocyte differentiation markers ADIPOQ and aP2. On the contrary, the d-ECM-coated substrate continuously promoted the expression of PPARγ, and regulated the phosphorylation of ERK1/2 in different manners during differentiation. Pretreatment with ERK1/2 inhibitor PD98059 neutralized the effects of d-ECM, which suggested d-ECM might regulate the adipogenesis of ADSCs through ERK1/2-PPARγ pathway. In addition, d-ECM was revealed to regulate the transcription and expression of stemness-associated genes, such as OCT4, NANOG and SOX2, in the undifferentiated ADSCs, which might be related to the initiation of differentiation.
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http://dx.doi.org/10.1080/21623945.2021.1906509DOI Listing
December 2021

Icaritin Provides Neuroprotection in Parkinson's Disease by Attenuating Neuroinflammation, Oxidative Stress, and Energy Deficiency.

Antioxidants (Basel) 2021 Mar 29;10(4). Epub 2021 Mar 29.

National Key Research Laboratory of Natural and Biomimetic Drugs, Peking University, Beijing 100191, China.

Neuroinflammation, oxidative stress, and mitochondrial dysfunction are all important pathogenic mechanisms underlying motor dysfunction and dopaminergic neuronal damage observed in patients with Parkinson's disease (PD). However, despite extensive efforts, targeting inflammation and oxidative stress using various approaches has not led to meaningful clinical outcomes, and mitochondrial enhancers have also failed to convincingly achieve disease-modifying effects. We tested our hypothesis that treatment approaches in PD should simultaneously reduce neuroinflammation, oxidative stress, and improve alterations in neuronal energy metabolism using the flavonoid icaritin in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) mouse model of PD. Using matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI), coupled with biochemical analyses and behavioral tests, we demonstrate that icaritin improves PD by attenuating the the NOD-like receptor family pyrin domain-containing protein 3 (NLRP3) inflammasome activity and stabilizing mitochondrial function, based on our extensive analyses showing the inhibition of NLRP3 inflammasome, reduction of NLRP3-mediated IL-1β secretion, and improvements in the levels of antioxidant molecules. Our data also indicated that icaritin stabilized the levels of proteins related to mitochondrial function, such as voltage-dependent anion channel (VDAC) and ATP synthase subunit beta (ATP5B), as well as those of molecules related to energy metabolism, such as ATP and ADP, ultimately improving mitochondrial dysfunction. By employing molecular docking, we also discovered that icaritin can interact with NLRP3, VDAC, ATP5B, and several blood-brain barrier (BBB)-related proteins. These data provide insights into the promising therapeutic potential of icaritin in PD.
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http://dx.doi.org/10.3390/antiox10040529DOI Listing
March 2021

Gold Nanoparticle-Enhanced Detection of DNA Hybridization by a Block Copolymer-Templating Fiber-Optic Localized Surface Plasmon Resonance Biosensor.

Nanomaterials (Basel) 2021 Mar 1;11(3). Epub 2021 Mar 1.

College of Physics, Dalian University of Technology, Dalian 116024, China.

To overcome low surface coverage and aggregation of particles, which usually restricts the sensitivity and resolution of conventional localized surface plasmon resonance (LSPR) fiber-optic sensors, we propose a simple self-assembled templating technique that uses a nanometer thickness block copolymer (BCP) layer of poly(styrene-b-4-vinylpyridine) to form a 33 nm gold nanoparticle (AuNP) monolayer with high uniformity and density for LSPR sensing. The LSPR resonance wavelength for this PS-b-P4VP templated methodology is 592 nm and its refractive index sensitivity is up to 386.36 nm/RIU, both of which are significantly improved compared to those of conventional LSPR techniques. Calibrated by a layer-by-layer polyelectrolyte deposition procedure, the decay length of this LSPR sensor is calculated to be 78 nm, which is lower than other traditional self-assembled LSPR sensors. Furthermore, hybridization between target ssDNA, which is linked with capture ssDNA on the LSPR biosensor and DNA-AuNP conjugates, leads to a low detection limit of 67 pM. These enhanced performances are significant and valuable for high-sensitivity and cost-effective LSPR biosensing applications.
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http://dx.doi.org/10.3390/nano11030616DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7998318PMC
March 2021

Solar-Powered Photodegradation of Pollutant Dyes Using Silver-Embedded Porous TiO Nanofibers.

Nanomaterials (Basel) 2021 Mar 27;11(4). Epub 2021 Mar 27.

Institute of Materials Research and Engineering, Agency for Science, Technology and Research (A*STAR), 2 Fusionopolis Way, Singapore 138634, Singapore.

Titanium dioxide (TiO) nanomaterials have been ubiquitously investigated as a photocatalyst for organic contaminant treatment in wastewater due to their exemplary semiconductor properties. However, their huge band gap remains a barrier for visible light absorption, limiting their utility in practical applications. The incorporation of noble metals in the TiO scaffold would help mitigate the problem via plasmonic resonance enhancements. Silver (Ag) is the chosen noble metal as it is relatively cheap and has great plasmonic effects. In this study, the use of electrospun Ag-embedded TiO nanofibers as a photocatalyst is shown to be effective in decomposing rhodamine B and methyl orange dyes under a solar simulator in 3 h, which is more efficacious as opposed to pristine TiO nanofibers. This showcases the potential of a simple and economic wastewater treatment system for the removal of organic pollutants.
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http://dx.doi.org/10.3390/nano11040856DOI Listing
March 2021

Dense-Stacking Porous Conjugated Polymer as Reactive-Type Host for High-Performance Lithium Sulfur Batteries.

Angew Chem Int Ed Engl 2021 Mar 9. Epub 2021 Mar 9.

Department of Chemistry, National University of Singapore, 3 Science Drive 3, 117543, Singapore, Singapore.

Commercialization of the lithium-sulfur battery is hampered by bottlenecks like low sulfur loading, high cathode porosity, uncontrollable Li S deposition and sluggish kinetics of Li S activation. Herein, we developed a densely stacked redox-active hexaazatrinaphthylene (HATN) polymer with a surface area of 302 m  g and a very high bulk density of ca. 1.60 g cm . Uniquely, HATN polymer has a similar redox potential window to S, which facilitates the binding of Li S and its transformation chemistry within the bulky polymer host, leading to fast Li S/S kinetics. The compact polymer/S electrode presents a high sulfur loading of ca. 15 mg  cm (200-μm thickness) with a low cathode porosity of 41 %. It delivers a high areal capacity of ca. 14 mAh cm and good cycling stability (200 cycles) at electrolyte-sulfur (E/S) ratio of 5 μL mg . The assembled pouch cell delivers a cell-level high energy density of 303 Wh kg and 392 Wh L .
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http://dx.doi.org/10.1002/anie.202016240DOI Listing
March 2021

Obesity and Mortality Among Patients Diagnosed With COVID-19: A Systematic Review and Meta-Analysis.

Front Med (Lausanne) 2021 5;8:620044. Epub 2021 Feb 5.

Graduate Institute of Biomedical Informatics, College of Medical Science and Technology, Taipei Medical University, Taipei, Taiwan.

Coronavirus disease 2019 (COVID-19) has already raised serious concern globally as the number of confirmed or suspected cases have increased rapidly. Epidemiological studies reported that obesity is associated with a higher rate of mortality in patients with COVID-19. Yet, to our knowledge, there is no comprehensive systematic review and meta-analysis to assess the effects of obesity and mortality among patients with COVID-19. We, therefore, aimed to evaluate the effect of obesity, associated comorbidities, and other factors on the risk of death due to COVID-19. We did a systematic search on PubMed, EMBASE, Google Scholar, Web of Science, and Scopus between January 1, 2020, and August 30, 2020. We followed Cochrane Guidelines to find relevant articles, and two reviewers extracted data from retrieved articles. Disagreement during those stages was resolved by discussion with the main investigator. The random-effects model was used to calculate effect sizes. We included 17 articles with a total of 543,399 patients. Obesity was significantly associated with an increased risk of mortality among patients with COVID-19 (RR: 1.42 (95%CI: 1.24-1.63, < 0.001). The pooled risk ratio for class I, class II, and class III obesity were 1.27 (95%CI: 1.05-1.54, = 0.01), 1.56 (95%CI: 1.11-2.19, < 0.01), and 1.92 (95%CI: 1.50-2.47, < 0.001), respectively). In subgroup analysis, the pooled risk ratio for the patients with stroke, CPOD, CKD, and diabetes were 1.80 (95%CI: 0.89-3.64, = 0.10), 1.57 (95%CI: 1.57-1.91, < 0.001), 1.34 (95%CI: 1.18-1.52, < 0.001), and 1.19 (1.07-1.32, = 0.001), respectively. However, patients with obesity who were more than 65 years had a higher risk of mortality (RR: 2.54; 95%CI: 1.62-3.67, < 0.001). Our study showed that obesity was associated with an increased risk of death from COVID-19, particularly in patients aged more than 65 years. Physicians should aware of these risk factors when dealing with patients with COVID-19 and take early treatment intervention to reduce the mortality of COVID-19 patients.
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http://dx.doi.org/10.3389/fmed.2021.620044DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7901910PMC
February 2021

Controlling Native Oxidation of HfS for 2D Materials Based Flash Memory and Artificial Synapse.

ACS Appl Mater Interfaces 2021 Mar 19;13(8):10639-10649. Epub 2021 Feb 19.

Joint School of National University of Singapore and Tianjin University, International Campus of Tianjin University, Binhai New City, Fuzhou 350207, P. R. China.

Two-dimensional (2D) materials based artificial synapses are important building blocks for the brain-inspired computing systems that are promising in handling large amounts of informational data with high energy-efficiency in the future. However, 2D devices usually rely on deposited or transferred insulators as the dielectric layer, resulting in various challenges in device compatibility and fabrication complexity. Here, we demonstrate a controllable and reliable oxidation process to turn 2D semiconductor HfS into native oxide, HfO, which shows good insulating property and clean interface with HfS. We then incorporate the HfO/HfS heterostructure into a flash memory device, achieving a high on/off current ratio of ∼10, a large memory window over 60 V, good endurance, and a long retention time over 10 seconds. In particular, the memory device can work as an artificial synapse to emulate basic synaptic functions and feature good linearity and symmetry in conductance change during long-term potentiation/depression processes. A simulated artificial neural network based on our synaptic device achieves a high accuracy of ∼88% in MNIST pattern recognition. Our work provides a simple and effective approach for integrating high- dielectrics into 2D material-based memory and synaptic devices.
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http://dx.doi.org/10.1021/acsami.0c22561DOI Listing
March 2021

Meta-analysis of P53 expression and sensitivity to platinum-based chemotherapy in patients with non-small cell lung cancer.

Medicine (Baltimore) 2021 Feb;100(5):e24194

Department of Respiratory and Critical Care Medicine, Fujian Provincial Hospital,Shengli Clinical Medical College of Fujian Medical University, Dongjie,Road No 134, Fuzhou, Fujian, China.

Background: The relationship between p53 expression and chemosensitivity of non-small cell lung cancer (NSCLC) is unclear. This study aims to explore the correlation between p53 expression and sensitivity to platinum-based chemotherapy in patients with NSCLC.

Methods: Pubmed, Web of Science, EMBASE, CNKI, China Wanfang databases were searched for studies on the relationship between the p53 expression and the chemosensitivity to platinum drugs in patients with NSCLC. The last search time was May 2020. Stata 15.0 software was used for statistical analysis.

Results: A total of 21 studies were included, covering 1387 patients in total. The results showed that the pooled OR = 1.55 (95%CI: 1.05∼2.29, P < .05), for Asian population, the pooled OR = 1.67 (95%CI: 0.95∼3.09, P > .05), for Caucasian population, the pooled OR = 1.34 (95%CI: 0.74∼2.43), there was no significant difference between Asian and Caucasian. The results of subgroup analysis of publication year showed that, the pooled OR = 2.07 (95%CI: 1.39∼3.07, P < .01), the heterogeneity among the studies decreased remarkably after 2005. The subgroup analysis of advanced patients showed that the pooled OR = 1.93 (95%CI: 1.27∼2.93), the difference was statistically significant.

Conclusion: Patients with p53 negative expression is more sensitive to platinum-based chemotherapy than those with p53 positive expression in NSCLC, especially in advanced NSCLC.
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http://dx.doi.org/10.1097/MD.0000000000024194DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7870161PMC
February 2021

MicroRNA-124-3p affects myogenic differentiation of adipose-derived stem cells by targeting Caveolin-1 during pelvic floor dysfunction in Sprague Dawley rats.

Ann Transl Med 2021 Jan;9(2):161

Department of Plastic Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.

Background: The aim of this study was to investigate using myogenic differentiation of adipose stem cells for the treatment of female pelvic floor dysfunction (PFD) and aimed to further study the influences of microRNA-124-3p (miR-124-3p) in the process of myogenic differentiation of adipose-derived stem cells (ADSCs) through targeting Caveolin-1 (Cav1) during PFD in Sprague Dawley (SD) rats.

Methods: The ADSCs were separated from 6-8-week-old female SD rats (n=25) and were cultivated. Then, we observed the cell status and conducted fat and osteogenic experiments. We then constructed an ADSC-green fluorescent protein (GFP) stable transfer strain. Flow cytometry was used to identify the positive rates of CD44, CD90, and CD45 in ADSCs and ADSC-GFP. Real-time quantitative polymerase chain reaction (qRT-PCR) and western blotting were used to mRNA and protein expression levels. Myogenic differentiation of ADSCs was measured with immunofluorescence methods. A dual-luciferase reporter assay was executed to affirm whether Cav1 was a target of miR-124-3p.

Results: The isolated ADSCs cells were in good condition under the microscope. The results of flow cytometry showed that the positive rate of CD44 and CD90 was high, and the positive rate of CD45 was low in ADSCs and ADSC-GFP. Under normal culture conditions, ADSCs-GFP cells can be massively adipated and osteogenic. After 5-Aza induced ADSC-GFP myogenic differentiation, the level of miR-124-3p was significantly increased. We found that MiR-124-3p mimics promoted the myogenic differentiation of ADSCs. Moreover, we discovered that Cav1 was a target gene of miR-124-3p and was negatively regulated by miR-124-3p. The results of leak point pressure (LPP), hematoxylin and eosin (HE), and Masson showed that the collagen fiber content of the PFD group was lower than that of the control group; the collagen fiber content of ADSC-GFP, 5-Aza, or miR-124-3p mimics were increased after intervention. Furthermore, the outcomes qRT-PCR, western blotting, and immunofluorescence suggested that miR-124-3p facilitated the survival ADSC-GFP fat transplantation by regulating many key factors in vivo.

Conclusions: These results proofed that miR-124-3p could accelerate myogenic differentiation of ADSCs by down-regulating Cav1 to improve PFD in SD rats, which will pave the way for therapeutic delivery of miRNA targeting PFD disease.
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http://dx.doi.org/10.21037/atm-20-8212DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7867888PMC
January 2021

Amblyopia and Refractive Status in Congenital Ptosis: The Effect and Timing of Surgical Correction.

Ann Plast Surg 2021 Feb 1. Epub 2021 Feb 1.

From the Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine Shanghai Key Laboratory of Orbital Diseases and Ocular Oncology, Shanghai, the People's Republic of China.

Purpose: The aim of this study was to explore the effect of congenital ptosis on the visual function of patients and observe the improvement in visual function after the correction of ptosis at different surgical times.

Methods: We performed a retrospective study of 265 patients (346 eyes) with congenital ptosis at the same hospital. Each patient underwent an ophthalmic examination, including optometry and measurement of the affected palpebral fissure height and upper eyelid margin reflex distance. Preoperative clinical and morphologic data were compared with postoperative data.

Results: The refractive error of the patient population was significantly different from that of the general population (P < 0.01). The rates of strabismus and amblyopia were 12.45% and 36.98%, respectively, in the ptosis patients, both of which are higher than the rates in the general population (P < 0.01), especially for unilaterally affected patients and patients with the optical axis covered. The refractive error of patients younger than 5 years was improved postoperatively (P < 0.05). The number of cases of amblyopia and strabismus was reduced, as was the percentage of cases of with-the-rule astigmatism (P < 0.05).

Conclusion: Congenital ptosis can result in refractive error, strabismus, and amblyopia, which could seriously impair the visual function of patients. The impairment is more pronounced in unilaterally affected patients. Surgery is conducive to the recovery of visual function, especially in patients younger than 5 years. Satisfactory curative effects can be obtained before the age of 3 years; thus, unilaterally affected patients, severely affected patients, and patients with impaired visual function should undergo surgery as soon as possible.
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http://dx.doi.org/10.1097/SAP.0000000000002677DOI Listing
February 2021

Effects of body postures on the shear modulus of thoracolumbar fascia: a shear wave elastography study.

Med Biol Eng Comput 2021 Feb 25;59(2):383-390. Epub 2021 Jan 25.

Luoyang Orthopedics Hospital of Henan Province, Luoyang City, Henan Province, China.

This study is aimed to use shear wave elastography (SWE) to study the relationship between shear modulus and different body postures of the thoracolumbar fascia (TLF) and acquire physiologically meaningful information from the stiffness-posture graph to better quantify passive flexion responses. Seven passive postures were defined to evaluate the shear modulus of right side TLF at the third and fourth lumbar vertebra levels (L3 and L4) in twenty healthy male subjects. The TLF stiffness was significantly different among different postures (p < 0.001), and the TLF stiffness at L3 was always less than that at L4 (p < 0.001). As the forward tilt increased from 0 to 60°, the TLF stiffness increased in sitting and standing postures by 54.01% and 192.84%. In the neutral postures, the TLF stiffness in standing and sitting postures was 66.98% and 165.48% higher than that in rest posture. The above results show that the elastic properties of TLF play an important role in maintaining body static posture and that the forward tilt and sitting postures are likely to induce low back pain (LBP). In conclusion, this study provides preliminary in vivo data for the relationship between body postures and TLF stiffness.
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http://dx.doi.org/10.1007/s11517-021-02320-2DOI Listing
February 2021

A Systematic Review of the Clinical Manifestations and Diagnostic Methods for Macular Coloboma.

Curr Eye Res 2021 Jan 22:1-6. Epub 2021 Jan 22.

Department of Ophthalmology, Faculty of Medicine and University Hospital Cologne, University of Cologne , Cologne, Germany.

: To present the clinical features of and diagnostic methods used for macular coloboma (MC), and to analyze the factors associated with best-corrected visual acuity (BCVA) in patients with MC. : A systematic review using the MEDLINE (PubMed), EMBASE, LILACS, and Cochrane databases was performed. The factors associated with BCVA were analyzed. : A total of 21 patients (mean age at diagnosis, 18.1 ± 14.6 years) with 36 eyes affected by MC (5 unilateral, 16 bilateral) were included in the study. All 21 patients (100%) had undergone a good-quality fundus examination. The size of the MC lesions ranged from 1.0 × 1.2 to 4.0 × 4.0 disc diameters (DD). Twenty-seven (73%) eyes had pigmented MC, seven (19%) had non-pigmented MC, and one (3%) had an unspecific type. The diagnosis was confirmed using spectral-domain optical coherence tomography (SD-OCT) in 16 (43.2%) eyes. A positive correlation was found between BCVA and the type of MC (β = 0.876, = .006) and abnormal eye movement (β = 0.087, = .018), and a negative correlation was found between BCVA and a contributory medical history of ventricular septal defect (β = -0.327, = .001). : Pigmented MC was the most common type and had the highest possibility of causing impaired vision in the affected eyes. Additionally, joint examinations should be applied for diagnostic confirmation of MC. Furthermore, fundoscopy, electroretinogram, electrooculography, fundus fluorescein angiography, and SD-OCT are all critical for differential diagnosis of MC-like lesions.
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http://dx.doi.org/10.1080/02713683.2020.1853779DOI Listing
January 2021

Development of immunoassay methods based on monoclonal antibody and its application in the determination of cadmium ion.

J Hazard Mater 2020 Dec 31;411:124992. Epub 2020 Dec 31.

The Ministry of Education Key Laboratory of Biopesticide and Chemical Biology, Fujian Key Laboratory of Pathogenic Fungi and Mycotoxins, and School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China. Electronic address:

Owing to the threat of cadmium (Cd) to public health, it is an urgent demand to develop effective, sensitive, and rapid methods for the detection of cadmium. In this study, indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and immunochromatographic test strips (ICTS) were established for the determination of Cd based on the obtained mAb with high specificity and high affinity (Kaff = 3.0 × 10 L/moL). The linear range of ic-ELISA detection was 0.03-1.11 ng/mL and 50% inhibitive concentration (IC50) of cadmium ion was determined to be 0.15 ng/mL. The visual limit of detection (vLOD) of the AuNS-based strip was 0.375 ng/mL. The vLOD of AuNF-based strip using higher intensity reporter determined to be 0.03 ng/mL, which was enhanced 12 times compared to the traditional strip. In summary, the developed immunoassays based on mAb shows great potential for monitoring the cadmium ion in environmental samples.
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http://dx.doi.org/10.1016/j.jhazmat.2020.124992DOI Listing
December 2020

Reliability of Stereophotogrammetry for Area Measurement in the Periocular Region.

Aesthetic Plast Surg 2021 Jan 15. Epub 2021 Jan 15.

Department of Ophthalmology, Faculty of Medicine and University Hospital Cologne, University of Cologne, Kerpener Strasse 62, 50937, Cologne, Germany.

Three-dimensional (3D) stereophotography area measurements are essential for describing morphology in the periocular region. However, its reliability has not yet been sufficiently validated. The objective of this study was to evaluate the reliability of 3D stereophotogrammetric area measurements in the periocular region. Forty healthy volunteers had five flat paper objects placed at each of the seven periocular positions including the endocanthion and the upper medial, upper middle, upper lateral, lower medial, lower middle, and the lower lateral eyelid. Two series of photographic images were captured twice by the same investigator. Each image of the first series was measured twice by the same rater, while images of both series were measured once by a second rater. Differences between these measurements were calculated, and the intrarater, interrater, and intramethod reliability was evaluated for intraclass correlation coefficients (ICCs), mean absolute differences (MADs), technical errors of measurements (TEMs), relative errors of measurements (REMs), and relative TEM (rTEM). Our results showed that 21.2% of all ICCs were considered as excellent, 45.5% were good, 27.3% were moderate, and 6.1% were poor. The interrater ICC for the endocanthion location was 0.4% on a low level. MAD values for all objects were less than 0.3 mm, all TEM were less than 1 mm, the REM and rTEM were less than 2% for all objects, showing high reliability. 3D stereophotogrammetry is a highly reliable system for periocular area measurements and may be used in the clinical routine for planning oculoplastic surgeries and for evaluating changes in periocular morphology.Level of Evidence IV This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266.
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http://dx.doi.org/10.1007/s00266-020-02091-5DOI Listing
January 2021

Clinical Characteristics and Neonatal Outcomes of Pregnant Patients With COVID-19: A Systematic Review.

Front Med (Lausanne) 2020 3;7:573468. Epub 2020 Dec 3.

College of Medical Science and Technology, Graduate Institute of Biomedical Informatics, Taipei Medical University, Taipei, Taiwan.

Coronavirus disease 2019 (COVID-19) characterized by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has created serious concerns about its potential adverse effects. There are limited data on clinical, radiological, and neonatal outcomes of pregnant women with COVID-19 pneumonia. This study aimed to assess clinical manifestations and neonatal outcomes of pregnant women with COVID-19. We conducted a systematic article search of PubMed, EMBASE, Scopus, Google Scholar, and Web of Science for studies that discussed pregnant patients with confirmed COVID-19 between January 1, 2020, and April 20, 2020, with no restriction on language. Articles were independently evaluated by two expert authors. We included all retrospective studies that reported the clinical features and outcomes of pregnant patients with COVID-19. Forty-seven articles were assessed for eligibility; 13 articles met the inclusion criteria for the systematic review. Data is reported for 235 pregnant women with COVID-19. The age range of patients was 25-40 years, and the gestational age ranged from 8 to 40 weeks plus 6 days. Clinical characteristics were fever [138/235 (58.72%)], cough [111/235 (47.23%)], and sore throat [21/235 (8.93%)]. One hundred fifty six out of 235 (66.38%) pregnant women had cesarean section, and 79 (33.62%) had a vaginal delivery. All the patients showed lung abnormalities in CT scan images, and none of the patients died. Neutrophil cell count, C-reactive protein (CRP) concentration, ALT, and AST were increased but lymphocyte count and albumin levels were decreased. Amniotic fluid, neonatal throat swab, and breastmilk samples were taken to test for SARS-CoV-2 but all found negativ results. Recent published evidence showed the possibility of vertical transmission up to 30%, and neonatal death up to 2.5%. Pre-eclampsia, fetal distress, PROM, pre-mature delivery were the major complications of pregnant women with COVID-19. Our study findings show that the clinical, laboratory and radiological characteristics of pregnant women with COVID-19 were similar to those of the general populations. The possibility of vertical transmission cannot be ignored but C-section should not be routinely recommended anymore according to latest evidences and, in any case, decisions should be taken after proper discussion with the family. Future studies are needed to confirm or refute these findings with a larger number of sample sizes and a long-term follow-up period.
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http://dx.doi.org/10.3389/fmed.2020.573468DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7772992PMC
December 2020

Gold-decorated TiO nanofibrous hybrid for improved solar-driven photocatalytic pollutant degradation.

Chemosphere 2021 Feb 25;265:129114. Epub 2020 Nov 25.

Institute of Materials Research and Engineering, Agency for Science, Technology and Research (A∗STAR), 2 Fusionopolis Way, 138634, Singapore. Electronic address:

TiO-based nanomaterials are among the most promising photocatalysts for degrading organic dye pollutants. In this work, Au-TiO nanofibers were fabricated by the electrospinning technique, followed by calcination in air at 500 °C. Morphological and structural analyses revealed that the composite consists of TiO nanofibers with embedded Au nanoparticles that are extensively distributed throughout the porous fibrous structure of TiO. The photocatalytic performance of these Au-embedded TiO nanofibers was evaluated in the photodegradation of Rhodamine B and methylene blue under solar simulator irradiation. Compared with pristine TiO nanofibers, the Au-embedded TiO nanofibers displayed far better photocatalytic degradation efficiency. The plasmon resonance absorption of Au nanoparticles in the visible spectral region and the effective charge separation at the heterojunction of the Au-TiO hybrid are the key factors that have led to the considerable enhancement of the photocatalytic activity. The results of this study clearly demonstrate the potential of Au-TiO electrospun nanofibers as solar-light-responsive photocatalysts for the effective removal of dye contaminants from aquatic environments.
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http://dx.doi.org/10.1016/j.chemosphere.2020.129114DOI Listing
February 2021

A Case of Tuberous Sclerosis Complex with Lymphangioleiomyomatosis and Renal Cell Carcinoma.

Onco Targets Ther 2020 2;13:12421-12426. Epub 2020 Dec 2.

Department of Respiratory Medicine, Fujian Provincial Hospital, Fujian Medical University, Fuzhou, Fujian 350001, People's Republic of China.

Lymphangioleiomyomatosis (LAM) is a rare disease affecting young women, which occurs sporadically or in patients with tuberous sclerosis complex (TSC). The main manifestations of TSC in the kidney include cysts and angiomyolipoma (AML). Although renal cell carcinoma (RCC) is not a manifestation of TSC, it has a 2-4% incidence in TSC patients. Furthermore, LAM is rare in patients with RCC. Herein, we present a case of a 40-year-old woman with LAM and RCC in the right kidney. We checked for mutations in the and genes from both blood and kidney lesions and found a heterozygous mutation of c.1717-30G> A in intron 16 of gene. In TSC patients, the diagnosis of RCC is challenging because the cancer is rare, and it is often difficult to distinguish it from AML with conventional imaging techniques. Therefore, it is recommended that patients with TSC undergo renal imaging follow-ups annually for kidney masses.
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http://dx.doi.org/10.2147/OTT.S276627DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7719313PMC
December 2020

Duodenal plasma cells correspond to serum IgA in common variable immunodeficiency.

Pathology 2020 Nov 17. Epub 2020 Nov 17.

Departments of Immunology and Immunopathology, Westmead Hospital, Sydney, NSW, Australia; The University of Sydney, Faculty of Medicine, Sydney, NSW, Australia; NSW Health Pathology, Sydney, NSW, Australia. Electronic address:

Common variable immunodeficiency (CVID) can be associated with a range of serum IgA concentrations, from absent, to variably reduced, and in some patients classified as 'possible CVID', even normal. The aim of this study was to assess the proportion of duodenal plasma cells in patients with CVID and determine whether there was an association with serum IgA concentration. Duodenal biopsies obtained at upper endoscopy from 35 patients with CVID were assessed for the presence of plasma cells and compared with serum IgA concentrations. A reduction or absence of duodenal plasma cells in 60% of patients with CVID and an association between the proportions of duodenal plasma cells and serum IgA concentrations was demonstrated. The presence of duodenal plasma cells associated with numbers of isotype switched memory B cells in the peripheral blood. A reduction in serum IgA over time was observed in 19% of CVID patients. The gastrointestinal tract provides a window into the immune system in CVID, and these results reinforce the association between gastrointestinal plasma cells and serum IgA concentrations. Preservation of gastrointestinal plasma cells and serum IgA in some patients with CVID, and the sequential decline of both in others, highlight the heterogeneity of this disorder.
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http://dx.doi.org/10.1016/j.pathol.2020.08.014DOI Listing
November 2020

2H-MoS on MoCT MXene Nanohybrid for Efficient and Durable Electrocatalytic Hydrogen Evolution.

ACS Nano 2020 Nov 13;14(11):16140-16155. Epub 2020 Nov 13.

Institute of Materials Research and Engineering, Agency for Science, Technology and Research (A*STAR), 2 Fusionopolis Way, Innovis, Singapore 138634, Singapore.

The development of highly efficient and durable earth-abundant hydrogen evolution reaction (HER) catalysts is crucial for the extensive implementation of the hydrogen economy. Members of the 2D MXenes family, particularly MoCT, have recently been identified as promising HER catalysts. However, their inherent oxidative instability in air and aqueous electrolyte solutions is hindering their widespread use. Herein, we present a simple and scalable method to circumvent adventitious oxidation in MoCT MXenes sulfidation to form a MoCT/2H-MoS nanohybrid. The intimate epitaxial coupling at the MoCT/2H-MoS nanohybrid interface afforded superior HER activities, requiring only 119 or 182 mV overpotential to yield -10 or -100 mA cm current densities, respectively. Density functional theory calculations reveal strongest interfacial adhesion was found within the nanohybrid structure as compared to the physisorbed nanohybrid, and the possibility to tune the HER overpotential through manipulating the extent of MXene sulfidation. Critically, the presence of 2H-MoS suppresses further oxidation of the MXene layer, enabling the nanohybrid to sustain industrially relevant current densities of over -450 mA cm with exceptional durability. Less than 30 mV overpotential degradation was observed after 10 continuous days of electrolysis at a fixed -10 mA cm current density or 100,000 successive cyclic voltammetry cycles. The exceptional HER durability of the MoCT/2H-MoS nanohybrid presents a major step forward to realize practical implementation of MXenes as noble metal free catalysts for broad-based applications in water splitting and energy conversion.
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http://dx.doi.org/10.1021/acsnano.0c08671DOI Listing
November 2020

Endoscopy-guided diode laser-assisted transcaruncular StopLoss Jones tube implantation for canalicular obstructions in primary surgery.

Graefes Arch Clin Exp Ophthalmol 2020 Dec 6;258(12):2809-2817. Epub 2020 Oct 6.

Department of Ophthalmology, University of Cologne, Faculty of Medicine and University Hospital Cologne, Cologne, Germany.

Purpose: To introduce and evaluate a minimally-invasive endoscopy-guided transcaruncular laser-assisted StopLoss Jones tube (SLJT) implantation technique for severe canalicular obstructions in primary surgeries.

Methods: We retrospectively identified 12 adult patients (12 eyes) with severe epiphora secondary to long-segment canalicular obstructions. All the 12 eyes underwent an endoscopy-guided transcaruncular SLJT implantation with an 810-nm diode laser's assistance as the primary surgical approach. Surgical and functional success rates, intraoperative and postoperative complications, as well as the need for secondary surgery, are evaluated.

Results: Primary surgical success was achieved in 11 of the 12 cases (92%); one patient (8%) required secondary surgery to replace an SLJT with a shorter one. Ultimately, all cases showed well-placed functioning tubes. Three of the 12 cases (25%) presented conjunctival scarring, conjunctival granulation tissue, with or without tube-associated irritation of the ocular surface. We observed no sink-in, extrusion, nor crack of the tube. Complete functional success was achieved in 83%, and moderate functional success in 17% of all patients. The functionally unsuccessful outcome was not present in this study.

Conclusion: Endoscopy-guided transcaruncular diode laser-assisted SLJT implantation seems to be a promising minimally invasive approach for primary treatment of severe canalicular dacryostenosis. This novel technique shows high functional success rates. It seems to avoid the risk of tube malposition and extrusion, septal and turbinate injury, nasal adhesion, drainage failure, ethmoiditis, postoperative bleeding, and cutaneous scars.
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http://dx.doi.org/10.1007/s00417-020-04942-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7677269PMC
December 2020

Complement Factor H Displays Opposite Expression Patterns Under Two Situations of Methamphetamine Administration: Acute Exposure and Chronic Dependence.

Neurosci Bull 2020 12 7;36(12):1558-1562. Epub 2020 Sep 7.

National Key Research Laboratory of Natural and Biomimetic Drugs, Peking University, Beijing, 100191, China.

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http://dx.doi.org/10.1007/s12264-020-00576-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7719133PMC
December 2020

Ophthalmological features and treatments in five cases of Waardenburg syndrome.

Exp Ther Med 2020 Oct 23;20(4):3072-3077. Epub 2020 Jul 23.

Department of Ophthalmology, Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, Shanghai 200011, P.R. China.

The present study aimed to investigate the ocular characteristics and treatment of Waardenburg syndrome (WS). A total of five patients with Waardenburg syndrome from our hospital, aged between 1 and 8 years, were included in the present study. The clinical data of these patients were analyzed, and the ocular manifestations and treatments were described. The general manifestations included hearing loss (1/5), broad high nasal root (2/5) and hypoplasia of alae nasi (2/5). Ophthalmological evaluations revealed ptosis (1/5), strabismus 1 (1/5), synophrys (2/5), telecanthus (5/5), iris hypopigmentation (5/5), high intraocular pressure (1/5) and choroidal hypopigmentation (1/5). For patients with characteristic external eye abnormalities, including ptosis, ocular plastic surgery was performed. For patients with only symptoms of iris heterochromia, no special treatment was required. The findings from the present study suggest that patients with WS may have several characteristic ocular manifestations. Abnormalities in the eyelid can be corrected by ocular plastic surgery, which is beneficial to children's physical and mental development.
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http://dx.doi.org/10.3892/etm.2020.9035DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7444341PMC
October 2020

MoS /Polymer Heterostructures Enabling Stable Resistive Switching and Multistate Randomness.

Adv Mater 2020 Oct 26;32(42):e2002704. Epub 2020 Aug 26.

Institute of Materials Research and Engineering, Agency for Science, Technology and Research (A*STAR), Innovis, 2 Fusionopolis Way, Singapore, 138634, Singapore.

Resistive random-access memories (ReRAMs) based on transition metal dichalcogenide layers are promising physical sources for random number generation (RNG). However, most ReRAM devices undergo performance degradation from cycle to cycle, which makes preserving a normal probability distribution during operation a challenging task. Here, ReRAM devices with excellent stability are reported by using a MoS /polymer heterostructure as active layer. The stability enhancement manifests in outstanding cumulative probabilities for both high- and low-resistivity states of the memory cells. Moreover, the intrinsic values of the high-resistivity state are found to be an excellent source of randomness as suggested by a Chi-square test. It is demonstrated that one of these cells alone can generate ten distinct random states, in contrast to the four conventional binary cells that would be required for an equivalent number of states. This work unravels a scalable interface engineering process for the production of high-performance ReRAM devices, and sheds light on their promising application as reliable RNGs for enhanced cybersecurity in the big data era.
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http://dx.doi.org/10.1002/adma.202002704DOI Listing
October 2020

Impact of Anxiety or Depression Symptoms on Propofol Requirements for Sedation in Females: A Prospective Cohort Study.

J Clin Pharmacol 2020 10 23;60(10):1376-1384. Epub 2020 May 23.

Department of Anesthesiology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China.

Mental disorders are thought to affect various clinical outcomes during the perioperative period. Among them, anxiety and depression are 2 of the most common types. However, the impacts of anxiety or depression on propofol requirements remain unclear. This study aimed to investigate the effects of anxiety or depression symptoms on the propofol requirements for sedation in females. This study recruited female patients aged 18 to 65 years, with American Society of Anesthesiologists physical status classification of 1 to 2, who were scheduled for hysteroscopic surgery under propofol-based intravenous anesthesia. The day before surgery, the Hospital Anxiety and Depression Scale (HADS) was used to assess the symptoms of anxiety and depression within the past 6 months. Target-controlled propofol was gradually titrated to achieve 3 desired levels of sedation: Modified Observer's Assessment of Alertness/Sedation scale (MOAA/S) score 3, MOAA/S score 1, and MOAA/S score 1 and Narcotrend Index <65. The effect-site concentration of propofol correlated with HADS-Anxiety scores for the sedation levels of MOAA/S 3 and 1 (r = .249, P = .008; and r = .190, P = .045, respectively). However, the propofol requirements did not correlate with HADS-Depression scores at any sedation level. In conclusion, female patients with anxiety symptoms, but not depression symptoms, required a higher dose of propofol for sedation in hysteroscopy.
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http://dx.doi.org/10.1002/jcph.1631DOI Listing
October 2020

Clinical efficacy and safety of somatostatin in the treatment of early postoperative inflammatory small bowel obstruction: A protocol for systematic review and meta analysis.

Medicine (Baltimore) 2020 May;99(20):e20288

Department of Critical Care Medicine, The Second Affiliated Hospital of Hainan Medical University, Longhua District, Haikou.

Background: As one of the complications after abdominal operation, early postoperative inflammatory small bowel obstruction (EPISBO) is a great trouble for many patients. The use of somatostatin in treating this disease had been widely reported, but its efficacy and safety were controversial. Therefore, the present research carried out a systematic review of the clinical efficacy and safety of somatostatin in treating EPISBO.

Methods: Computer retrieval was conducted in foreign databases (including PubMed, The Cochrane Library, and Embase) and Chinese database (including Sino Med, CNKI, VIP, and WangFang Data), supplementary search for the literatures included was performed, and manual retrieval was performed in abstracts, books, and non-electronic magazines related to the present research to ensure the recall rate. Among all republished relevant experimental studies in Chinese and English from January 1, 1996 to February 1, 2020, randomized controlled trials on the curative efficacy of somatostatin for EPISBO were collected. The evaluation measures included patients' total effective rate, peristaltic sound recovery time, time of disappearance of abdominal pain, time of first defecation after operation, drainage of gastrointestinal decompression and length of stay after treatment. The literatures were selected by two investigators independently to extract data according to the inclusion and exclusion criteria, Bias Risk Assessment Tool recommended by Cochrane Review Hand book 5.2 was used to assess literature quality, and meta-analysis was carried out by using soft Stata 12.0.

Results: This study will scientifically and effectively analyze the clinical efficacy and safety of somatostatin in the treatment of EPISBO through systematic review and meta-analysis.

Ethics And Dissemination: The results of this study will be published in a peer-reviewed SCI journal to provide evidence-based medical evidence for the clinical treatment of early postoperative inflammatory bowel obstruction.

Protocol And Registration: Open Science Framework (https://osf.io, OSF), registration number: ryd2g.
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http://dx.doi.org/10.1097/MD.0000000000020288DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7253791PMC
May 2020

Surrounded catalysts prepared by ion-exchange inverse loading.

Sci Adv 2020 May 13;6(20):eaay7031. Epub 2020 May 13.

Key Lab of Mesoscopic Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.

The supported catalyst featuring highly dispersed active phase on support is the most important kind of industrial catalyst. Extensive research has demonstrated the critical role (in catalysis) of the interfacial interaction/perimeter sites between the active phase and support. However, the supported catalyst prepared by traditional methods generally presents low interface density because of limit contact area. Here, an ion-exchange inverse loading (IEIL) method has been developed, in which the precursor of support is controllably deposited onto the precursor of active phase by ion-exchange reaction, leading to an active core surrounded (by support) catalyst with various structures. The unique surrounded structure presents not only high interface density and mutually changed interface but also high stability due to the physical isolation of active phase, revealing superior catalytic performances to the traditional supported catalysts, suggesting the great potential of this new surrounded catalyst as the upgrade of supported catalyst in heterogeneous catalysis.
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http://dx.doi.org/10.1126/sciadv.aay7031DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7220309PMC
May 2020

Self-Driving Car Location Estimation Based on a Particle-Aided Unscented Kalman Filter.

Sensors (Basel) 2020 Apr 29;20(9). Epub 2020 Apr 29.

Department of Electrical Engineering, University of Ulsan, 93 Daehak-ro, Nam-gu, Ulsan 44610, Korea.

Localization is one of the key components in the operation of self-driving cars. Owing to the noisy global positioning system (GPS) signal and multipath routing in urban environments, a novel, practical approach is needed. In this study, a sensor fusion approach for self-driving cars was developed. To localize the vehicle position, we propose a particle-aided unscented Kalman filter (PAUKF) algorithm. The unscented Kalman filter updates the vehicle state, which includes the vehicle motion model and non-Gaussian noise affection. The particle filter provides additional updated position measurement information based on an onboard sensor and a high definition (HD) map. The simulations showed that our method achieves better precision and comparable stability in localization performance compared to previous approaches.
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http://dx.doi.org/10.3390/s20092544DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7249166PMC
April 2020

Long noncoding RNA PLAC2 regulates PTEN in retinoblastoma and participates in the regulation of cancer cell apoptosis.

Oncol Lett 2020 Mar 17;19(3):2489-2494. Epub 2020 Jan 17.

Department of Ophthalmology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, P.R. China.

Long noncoding RNA placenta-specific 2 (PLAC2) blocks the cancer cell cycle in glioma, suggesting its tumor-suppressive role. The present study aimed to investigate the role of PLAC2 in retinoblastoma (Rb). It was found that PLAC2 was downregulated in Rb tissues and was not affected by the development of Rb. PTEN was also downregulated in Rb and positively correlated with PLAC2. In Rb cells, PLAC2 over-expression resulted in the upregulated expression of PTEN, while PTEN over-expression did not affect PLAC2 expression. PLAC2 and PTEN over-expression caused an increased apoptotic rate of Rb cells. PTEN small interfering RNA silencing led to a decreased apoptotic rate and attenuated the effects of PLAC2 over-expression. Therefore, PLAC2 regulates PTEN in Rb and participates in the regulation of cancer cell apoptosis.
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http://dx.doi.org/10.3892/ol.2020.11314DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7039102PMC
March 2020

Development of Novel Mixed Halide/Superhalide Tin-Based Perovskites for Mesoscopic Carbon-Based Solar Cells.

J Phys Chem Lett 2020 Apr 13;11(7):2443-2448. Epub 2020 Mar 13.

Department of Applied Chemistry and Institute of Molecular Science, National Chiao Tung University, 1001 Ta-Hsueh Road, Hsinchu 30010, Taiwan.

Tin perovskites suffer from poor stability and a self-doping effect. To solve this problem, we synthesized novel tin perovskites based on superhalide with varied ratios of tetrafluoroborate to iodide and implemented them into solar cells based on a mesoscopic carbon-electrode architecture because film formation was an issue in applying this material for a planar heterojunction device structure. We undertook quantum-chemical calculations based on plane-wave density functional theory (DFT) methods and explored the structural and electronic properties of tin perovskites FASnI(BF) in the series = 0, 1, 2, and 3. We found that only the = 2 case, FASnI(BF), was successfully produced, beyond the standard FASnI. The electrochemical impedance and X-ray photoelectron spectra indicate that the addition of tin tetrafluoroborate instead of SnI suppressed trap-assisted recombination by decreasing the Sn content. The power conversion efficiency of the FASnI(BF) device with FAI and Sn(BF) in an equimolar ratio improved 72% relative to that of a standard FASnI solar cell, with satisfactory photostability under ambient air conditions.
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http://dx.doi.org/10.1021/acs.jpclett.0c00479DOI Listing
April 2020

Robust RNA-Seq of aRNA-amplified single cell material collected by patch clamp.

Sci Rep 2020 02 6;10(1):1979. Epub 2020 Feb 6.

Zhilka Neurogenetic institute, University of Southern California, 1501 San Pablo St, Los Angeles, CA, 90033, USA.

Most single cell RNA sequencing protocols start with single cells dispersed from intact tissue. High-throughput processing of the separated cells is enabled using microfluidics platforms. However, dissociation of tissue results in loss of information about cell location and morphology and potentially alters the transcriptome. An alternative approach for collecting RNA from single cells is to re-purpose the electrophysiological technique of patch clamp recording. A hollow patch pipette is attached to individual cells, enabling the recording of electrical activity, after which the cytoplasm may be extracted for single cell RNA-Seq ("Patch-Seq"). Since the tissue is not disaggregated, the location of cells is readily determined, and the morphology of the cells is maintained, making possible the correlation of single cell transcriptomes with cell location, morphology and electrophysiology. Recent Patch-Seq studies utilizes PCR amplification to increase amount of nucleic acid material to the level required for current sequencing technologies. PCR is prone to create biased libraries - especially with the extremely high degrees of exponential amplification required for single cell amounts of RNA. We compared a PCR-based approach with linear amplifications and demonstrate that aRNA amplification (in vitro transcription, IVT) is more sensitive and robust for single cell RNA collected by a patch clamp pipette.
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http://dx.doi.org/10.1038/s41598-020-58715-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7004989PMC
February 2020