Publications by authors named "Ming Guan"

274 Publications

A patient with pseudohypoparathyroidism type 1A previously misdiagnosed as hereditary multiple exostosis: A case report.

Exp Ther Med 2022 Sep 28;24(3):597. Epub 2022 Jul 28.

Department of Pediatrics, Hangzhou Children's Hospital, Hangzhou, Zhejiang 310014, P.R. China.

Pseudohypoparathyroidism type 1A (PHP1A), a rare hereditary disorder, is featured by end-organ resistance to parathyroid hormone and Albright's hereditary osteodystrophy. Heterozygous mutation of guanine nucleotide-binding protein α stimulating () gene causes the half decreased bioactivity of the Gsα protein levels. Due to the diverse early clinical manifestations of PHP1A, a diagnosis of PHP1A is often easily overlooked and misdiagnosis or missed diagnosis is common. The present study described a girl who was initially diagnosed with hereditary multiple exostoses, but was afterwards confirmed with PHP1A. Moreover, genetic analysis indicated a new mutation (c2277deIC) of the gene.
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http://dx.doi.org/10.3892/etm.2022.11534DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9353500PMC
September 2022

First Report of , , and Carrying Showing High-Level Resistance to Carbapenems.

Front Microbiol 2022 7;13:916304. Epub 2022 Jul 7.

Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai, China.

The prevalence of carbapenem-resistant is increasing. Although carbapenemase production is the main resistance mechanism of to carbapenems, there are still some reports of non-carbapenemase-producing showing high-level resistance to carbapenems. In this study, we had also isolated a carbapenemase-negative carbapenem-resistant L204 from a patient with an asymptomatic urinary tract infection. Species identification was performed using MALDI-TOF MS, and carbapenemase-encoding genes were detected using both NG-test carba-5 and whole-genome sequencing. Antimicrobial susceptibility testing was performed by the broth microdilution method according to CLSI guidance. The results of antimicrobial susceptibility testing indicated that L204 was resistant to meropenem (MIC = 16 mg/L) and imipenem (MIC = 4 mg/L), but susceptible to ceftazidime-avibactam (MIC = 8 mg/L). Through whole-genome sequencing, several resistance genes had been identified, including , , , , , , , , , and . The efflux pump inhibition testing showed that the efflux pump was not involved in the resistance mechanism to carbapenems. The result of the conjugation experiment indicated that the plasmid with and was transferrable. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) demonstrated that L204 only contained outer membrane porin OmpK35.
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http://dx.doi.org/10.3389/fmicb.2022.916304DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9301006PMC
July 2022

APRIL and BAFF play a key role in differentiating vitreoretinal lymphoma from uveitis.

Clin Chim Acta 2022 Jul 9;535:1-6. Epub 2022 Jul 9.

Department of Ophthalmology, Huashan Hospital, Fudan University, Shanghai, China.

Background: Early diagnosis of vitreoretinal lymphoma (VRL) is critical for the successful treatment of this rare intraocular malignancy. However, fast and reliable diagnosis of VRL in patients presenting with intermediate or posterior non-infectious uveitis remains a challenge. A proliferation-inducing ligand (APRIL) and B-cell activating factor (BAFF) are vital factors in the pathophysiology, diagnosis, and prognosis of primary central nervous system lymphoma (PCNSL) and systemic autoimmune diseases. However, their utility as biomarkers for the diagnosis of VRL and uveitis remains unclear.

Methods: In this retrospective study, we analyzed APRIL and BAFF levels in the aqueous humor (AH) of 43 eyes of 40 patients, including 23 eyes of 20 patients with VRL, eight eyes of eight patients with uveitis, and 12 eyes of 12 patients with other ocular diseases (OODs). Additionally, we measured their levels after induction of chemotherapy in five eyes of five patients with VRL.

Results: AH levels of APRIL reliably distinguished VRL from uveitis, with a specificity of 78.3% and sensitivity of 75%. BAFF also showed similar potential. Serial AH analysis of patients with VRL during chemotherapy demonstrated a corresponding decline in AH levels of APRIL and BAFF.

Conclusion: This study extends the spectrum of valuable diagnostic biomarkers for VRL and uveitis. In patients with uveitis, the assessment of AH APRIL may help accelerate the diagnosis of VRL. Moreover, our results underline the important role of APRIL and BAFF in therapeutic monitoring of VRL.
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http://dx.doi.org/10.1016/j.cca.2022.06.014DOI Listing
July 2022

A Combined Cyanine/Carbomer Gel Enhanced Photodynamic Antimicrobial Activity and Wound Healing.

Nanomaterials (Basel) 2022 Jun 24;12(13). Epub 2022 Jun 24.

Spine Lab, Department of Orthopedic Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.

As a non-invasive and non-specific therapeutic approach, photodynamic therapy (PDT) has been used to treat antibiotic-resistant bacteria with encouraging efficacy. Inspired by light, the photosensitizers can produce excessive reactive oxygen species (ROS) and, thus, effectively destroy or kill bacteria. Cyanine (Cy), a traditional photosensitizer for PDT, has the advantages of low cytotoxicity and high ROS yield. Yet, the water solubility and photostability for Cy are poor, which substantially limit its antibacterial efficiency and clinical translation. Herein, we combined Cy with carbomer gel (CBMG) to form a photodynamic Cy-CBMG hydrogel. In this system, Cy was evenly dispersed in CBMG, and CBMG significantly improved the water solubility and photostability of Cy via electrostatic interactions. The developed Cy-CBMG gel had less photodegradation under laser irradiation and thus can effectively elevate ROS accumulation in bacteria. The Cy-CBMG compound presented remarkable ROS-induced killing efficacy against methicillin-resistant (93.0%) and extended-spectrum -lactamase-producing (88.7%) in vitro. Moreover, as a potential wound dressing material, the Cy-CBMG hydrogel exhibited excellent biocompatibility and effective antimicrobial ability to promote wound healing in vivo. Overall, this work proposed a practical strategy to synthesize a photosensitizer-excipient compound to enhance the photophysical property and antibacterial efficacy for PDT.
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http://dx.doi.org/10.3390/nano12132173DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9268119PMC
June 2022

Rapid and sensitive detection of amphetamine by SERS-based competitive immunoassay coupled with magnetic separation.

Anal Methods 2022 07 7;14(26):2608-2615. Epub 2022 Jul 7.

College of Chemistry and Chemical Engineering, Xinjiang Normal University, Urumqi 830054, China.

Amphetamine (AMP), as a psychiatric drug acting on the central nervous system, and has become one of the most common drugs of abuse in the illegal market at present, which adversely affects social public safety. We developed a SERS magnetic immunoassay with high sensitivity, specificity, and rapid and quantitative detection of AMP. We synthesized a high SERS intensity substrate ([email protected]) using the "hot spot" effect and combined it with antibodies to form SERS immunotags ([email protected]). Subsequently, the carboxyl magnetic beads were linked to label antigens as functional magnetic beads (carboxyl magnetic beads-AMP-BSA). Using the principle of competitive immunoassay, the Raman response value of the immune complex formed with SERS tags and functional magnetic beads was detected to realize the quantitative detection of AMP. The detection limit of this method for AMP was 2.28 ng mL. More importantly, a portable Raman instrument was used in this study, which can meet the requirements of point-of-care testing (POCT). Therefore, this SERS-based magnetic immunoassay can provide a favorable scientific basis for the control of drug abuse, monitoring by law enforcement agencies, and determination of drug users.
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http://dx.doi.org/10.1039/d2ay00581fDOI Listing
July 2022

Application of cerebrospinal fluid free light chain in diagnosis of primary central nervous system lymphoma and monitoring of associated chemotherapy efficacy.

Clin Chim Acta 2022 Aug 16;533:48-52. Epub 2022 Jun 16.

Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, 200040 Shanghai, China. Electronic address:

Background: Cerebrospinal fluid (CSF) free light chain (FLC) detection has been proposed as a tool for diagnosing primary central nervous system lymphoma (PCNSL), but there is no consensus on the appropriate reference range and its value for monitoring chemotherapy efficacy has not been investigated in Chinese PCNSL patients. We assessed the application potential of CSF FLC ratios for diagnosing PCNSL and monitoring associated treatment efficacy.

Methods: Kappa (κ) and lambda (λ) FLC were measured by nephelometry in CSF samples of patients with PCNSL (n = 45), other neurological diseases (n = 30), and normal controls (n = 60). Results of κ/λ FLC ratios (FLCr) were correlated with patients' diagnoses and receiver operating characteristic analysis was used to determine accuracy. In PCNSL patients, FLCr analysis was compared between PCNSL before and after treatment.

Results: κ FLC and FLCr concentrations in PCNSL were significantly higher than in patients without PCNSL (P < 0.05). The optimal cut-off for FLCr was 0.35, with diagnostic sensitivity and specificity of 78% and 72%, respectively. FLCr concentrations decreased after chemotherapy.

Conclusion: CSF FLC is a novel biomarker for diagnosis and chemotherapy efficacy monitoring in PCNSL.
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http://dx.doi.org/10.1016/j.cca.2022.06.011DOI Listing
August 2022

Establishment and Phenotypic Analysis of the Novel Gaucher Disease Mouse Model With the Partially Humanized Gene and F213I Mutation.

Front Genet 2022 27;13:892457. Epub 2022 May 27.

Key Laboratory of Metabolism and Molecular Medicine, Ministry of Education, Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai, China.

Gaucher disease (GD) is an autosomal recessive lysosomal storage disorder caused by mutations in the gene, which produces the glucocerebrosidase (GCase) protein. There are more than 500 mutations reported in , among which L444P (p.Leu444Pro) and F213I (p.Phe213Ile) are the most common in the Chinese population, while the function of F213I mutation remains elusive. This study aims to establish the GD mouse model of partially humanized gene with F213I mutation. GCase activity assays showed that the product of partially humanized gene, in which the mouse exons 5-7 were replace by the corresponding human exons, displayed similar activity with the wild-type mouse , while the F213I mutation in the humanized led to significant decrease in enzyme activity. ES cell targeting was used to establish the mice expressing the partially humanized -F213I. mice did not show obviously abnormal phenotypes, but homozygous mice died within 24 h after birth, whose epidermal stratum corneum were abnormal from the wild-type. The GCase activity in mice greatly decreased. In conclusion, our results showed that the partially humanized GD mouse model with the F213I mutation was developed and homozygous F213I mutation is lethal for newborn mice.
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http://dx.doi.org/10.3389/fgene.2022.892457DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9196271PMC
May 2022

Single-cell RNA sequencing reveals the characteristics of cerebrospinal fluid tumour environment in breast cancer and lung cancer leptomeningeal metastases.

Clin Transl Med 2022 06;12(6):e885

Department of Laboratory Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Leptomeningeal metastases (LM) occur in patients with breast cancer (BC) and lung cancer (LC) showing exceptionally poor prognosis. The cerebrospinal fluid (CSF) tumour microenvironment (TME) of LM patients is not well defined at a single-cell level. Based on the 10× genomics single-cell RNA sequencing (scRNA-seq) data from GEO database including five patient-derived CSF samples of BC-LM and LC-LM, and four patient-derived CSF samples of idiopathic intracranial hypertension (IIH) as controls, we analysed single-cell transcriptome characteristics of CSF TME in LM patients compared to controls simultaneously and comprehensively. In addition, we performed 10× genomics scRNA-seq on CSF cells derived from a BC-LM patient to help generate a solid conclusion. The CSF macrophages in LM patients showing M2-subtype signature and the emergence of regulatory T cells in LM confirmed the direction of tumour immunity toward immunosuppression. Then, the characteristics of CSF circulating tumour cells (CTCs) of breast cancer LM (BC-LM) patients were classified into five molecular subtypes by PAM50 model. The communication between macrophages and five subtype-specific CSF-CTCs showed largest number of ligand-receptor interactions. The five subtypes-specific CSF-CTCs showed great heterogeneities which were manifested in cell proliferation and cancer-testis antigens expression. Gene regulatory networks (GRNs) analysis revealed that transcription factor SREBF2 was universally activated in the five subtypes-specific CSF-CTCs. Our results will provide inspiration on new directions of the mechanism research, diagnosis and therapy of LM.
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http://dx.doi.org/10.1002/ctm2.885DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9178395PMC
June 2022

Poly(N-Isopropylacrylamide) Based Electrically Conductive Hydrogels and Their Applications.

Gels 2022 May 1;8(5). Epub 2022 May 1.

Department of Orthopedic Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.

Poly(N-isopropylacrylamide) (PNIPAM) based electrically conductive hydrogels (PNIPAM-ECHs) have been extensively studied in recent decades due to their thermal-responsive (leading to the volume change of hydrogels) and electrically conductive performance. The incorporation of conductive components into the PNIPAM hydrogel network makes it become conductive hydrogel, and as a result, the PNIPAM hydrogel could become sensitive to an electrical signal, greatly expanding its application. In addition, conductive components usually bring new stimuli-responsive properties of PNIPAM-based hydrogels, such as near-infrared light and stress/strain responsive properties. PNIPAM-ECHs display a wide range of applications in human motion detection, actuators, controlled drug release, wound dressings, etc. To summarize recent research advances and achievements related to PNIPAM-ECHs, this manuscript first reviews the design and structure of representative PNIPAM-ECHs according to their conductive components. Then, the applications of PNIPAM-ECHs have been classified and discussed. Finally, the remaining problems related to PNIPAM-ECHs have been summarized and a future research direction is proposed which is to fabricate PNIPAM-ECHs with integrated multifunctionality.
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http://dx.doi.org/10.3390/gels8050280DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9142127PMC
May 2022

Lumbosacral Transitional Vertebra Contributed to Lumbar Spine Degeneration: An MR Study of Clinical Patients.

J Clin Med 2022 Apr 22;11(9). Epub 2022 Apr 22.

Spine Lab, Department of Orthopedic Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.

We aimed to comprehensively characterize degenerative findings associated with various types of lumbosacral transitional vertebra (LSTV) on magnetic resonance images. Three hundred and fifty patients with LSTV (52.3 ± 10.9 years), including 182 Castellvi type I, 107 type II, 43 type III, and 18 type IV, and 179 controls without LSTV (50.6 ± 13.1 years), were studied. Discs, endplates, and posterior vertebral structures were assessed and compared to those of controls for the most caudal three discs on MRIs. There were no differences in degenerative findings between patients with type I LSTV and controls. For types III and IV, the transitional discs had smaller sizes, lower Pfirrmann scores, and lower rates of disc bulging (2.3% and 5.6% vs. 39.1%), osteophytes (2.3% vs. 15.1%), disc herniation (2.3% and 5.6% vs. 31.8%), and Modic changes (2.3% and 5.6% vs. 16.8%) than controls. However, the cranial discs had more severe Pfirrmann scores, disc narrowing and spinal canal narrowing, and greater rates of disc herniation (41.9% and 50.0% vs. 25.7%), endplate defects (27.9% and 33.3% vs. 14.4%) and spondylolisthesis (18.6% vs. 7.3%) than controls. Type II LSTV was associated with degenerative findings in the cranial segments but to a lesser degree, as compared with type III/IV LSTV. Thus, Castellvi type III/IV LSTV predisposed the adjacent spinal components to degeneration and protected the transitional discs. Type II LSTV had significant effects in promoting transitional and adjacent disc degeneration. Type I LSTV was not related to spinal degeneration.
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http://dx.doi.org/10.3390/jcm11092339DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9104340PMC
April 2022

Association between ethnicity and health knowledge among the floating population in China.

Cost Eff Resour Alloc 2022 Apr 2;20(1):15. Epub 2022 Apr 2.

Family Issues Center, Xuchang University, Xuchang, Henan, China.

Background: Health equity remains a priority concerns by central government in China. This study aimed to explore ethnic gaps in access to health knowledge categories and sources based on the survey data from a publicly available dataset.

Methods: Data were from 2015 China Migrants Dynamic Survey issued by The National Health Commission in China. Descriptive analyses were performed to reflect geodemographic differences in the floating population of ethnic minority (EMFP) and Han majority (HMFP) with Chi-square test. Ethnic gaps in access to health knowledge categories and sources were explored with Poisson regressions, logistic regressions, and bivariate ordered probit regressions.

Results: In the sample, most of participants had inadequate health information literacy. There were significant differences regarding geodemographic factors between EMFP and HMFP. Illiterate EMFP had likelihood to obtain less health knowledge categories (IRR = 0.80, 95% CI 0.77-0.84) and sources (IRR = 0.83, 95% CI 0.80-0.86) as compared to illiterate HMFP. Most of correlations between health knowledge categories and sources were weak in the samples of EMFP and HMFP.

Conclusion: Ethnic disparities in access to health knowledge categories and sources among the floating population in China were confirmed. Further effective efforts should be provided to reduce ethnic disparities in access to health knowledge under the ethnicity-orientated support of public health resource.
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http://dx.doi.org/10.1186/s12962-022-00349-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8976962PMC
April 2022

Bimetallic nitrogen-doped porous graphene for highly efficient magnetic solid phase extraction of 5-nitroimidazoles in environmental water.

Anal Chim Acta 2022 Apr 8;1203:339698. Epub 2022 Mar 8.

CAS Key Laboratory of Chemistry of Northwestern Plant Resources, Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, 730000, China. Electronic address:

In this work, Fe/Ni bimetallic nitrogen-doped porous graphene (Fe/Ni-NPG) nanomaterials with rich pores, strong magnetism and good reusability were successfully prepared by one-step combustion and can be used as magnetic solid phase extraction (MSPE) adsorbents for the determination of 5-nitroimidazoles (5-NDZs) in environmental water samples. The dispersion and active sites of the materials were increased by the introduction of nitrogen. The adsorption behavior of Fe/Ni-NPG for 5-NDZs was investigated, which corresponds to the quasi-second-order kinetics and Langmuir adsorption model. The π-π electron donor-acceptor interaction (π-π EDA), hydrogen bond and electrostatic interaction between Fe/Ni-NPG and 5-NDZs are the main factors that help to obtain excellent adsorption properties. Several important conditions of MSPE are systematically optimized. Under the optimal MSPE conditions, the linear range of DMZ was 0.6-500 μg/L, the linear range of TNZ and ONZ was 0.7-500 μg/L, and the correlation coefficient R ≥ 0.9991. The limit of detection was 0.18-0.2 μg/L, the limit of quantification was 0.6-0.7 μg/L, and the RSDs of intraday and interday precision were 1.58%-4.66% and 3.77-9.69%, respectively. In the three spiked actual environmental water samples, the recovery was 78.05%-107.05% (RSDs<7.82%). The results show that this method based on Fe/Ni-NPG provides an accurate and reliable way to detect 5-NDZs in environmental water.
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http://dx.doi.org/10.1016/j.aca.2022.339698DOI Listing
April 2022

High Spatial and Temporal Resolution NIR-IIb Gastrointestinal Imaging in Mice.

Nano Lett 2022 04 24;22(7):2793-2800. Epub 2022 Mar 24.

Nanophotonics Research Center, Shenzhen Key Laboratory of Micro-Scale Optical Information Technology & Institute of Microscale Optoelectronics, Shenzhen University, Shenzhen, Guangdong 518055, China.

Conventional biomedical imaging modalities, including endoscopy, X-rays, and magnetic resonance, are invasive and insufficient in spatial and temporal resolutions for gastrointestinal (GI) tract imaging to guide prognosis and therapy. Here we report a noninvasive method based on lanthanide-doped nanocrystals with ∼1530 nm fluorescence in the near-infrared-IIb window (NIR-IIb, 1500-1700 nm). The rational design of nanocrystals have led to an absolute quantum yield (QY) up to 48.6%. Further benefiting from the minimized scattering through the NIR-IIb window, we enhanced the spatial resolution to ∼1 mm in GI tract imaging, which is ∼3 times higher compared with the near-infrared-IIa (NIR-IIa, 1000-1500 nm) method. The approach also realized a high temporal resolution of 8 frames per second; thus the moment of mice intestinal peristalsis can be captured. Furthermore, with a light-sheet imaging system, we demonstrated a three-dimensional (3D) imaging on the GI tract. Moreover, we successfully translated these advances to diagnose inflammatory bowel disease.
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http://dx.doi.org/10.1021/acs.nanolett.1c04909DOI Listing
April 2022

promoter hypermethylation as a biomarker for the leukemic transformation of myeloproliferative neoplasms.

Epigenomics 2022 04 9;14(7):391-403. Epub 2022 Mar 9.

Department of Laboratory Medicine, Huashan Hospital, Fudan University, Shanghai, 200040, China.

To characterize the actionable biomarker for leukemic transformation (LT) of myeloproliferative neoplasms (MPNs) at the DNA damage repair promoter methylation level. Bioinformatic analysis and experimental validation were performed to identify the MPNs-LT specific biomarker out of the promoter methylation of 236 DNA damage repair genes with GSE42042 dataset and an in-house cohort of 80 MPNs. Hypermethylation of promoter was characterized as the mutation-independent epigenetic marker for MPNs-LT and repressed mRNA and protein expression, leading to olaparib hypersensitivity in the leukemic cells from MPNs-LT. Expressional silence of by promoter methylation compels the homologous recombination deficiency and vulnerability to PARP inhibition and serves as an actionable marker for targeted therapy for MPNs-LT.
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http://dx.doi.org/10.2217/epi-2022-0025DOI Listing
April 2022

Correction to: Sexual and reproductive health knowledge, sexual attitudes, and sexual behaviour of university students: Findings of a Beijing-Based Survey in 2010-2011.

Authors:
Ming Guan

Arch Public Health 2022 Feb 28;80(1):70. Epub 2022 Feb 28.

International Issues Center, Xuchang University, Road Bayi, Xuchang, 88, Henan, China.

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http://dx.doi.org/10.1186/s13690-021-00784-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8883718PMC
February 2022

Octahedral Pt-MOF with Au deposition for plasmonic effect and Schottky junction enhanced hydrogenothermal therapy of rheumatoid arthritis.

Mater Today Bio 2022 Jan 8;13:100214. Epub 2022 Feb 8.

Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou, Jiangsu, 221002, China.

Hydrogen (H) therapy is a novel and rapidly developing strategy utilized to treat inflammatory diseases. However, the therapeutic efficacy of H is largely limited with on-target off-synovium toxic effect, nonpolarity and low solubility. Herein, an intelligent H nanogenerator based upon the metal-organic framework (MOF) loaded with polydopamine and Perovskite quantum dots is constructed for the actualization of hydrogenothermal therapy. The biodegradable polydopamine with excellent photothermal conversion efficiencies is used for photothermal therapy (PTT) of rheumatoid arthritis (RA) and perovskite quantum dots (QDs) with unique photophysical properties are used as fluorescent signals for positioning [email protected]@QDs/PDA nanoparticles. In addition, the [email protected]@QDs/PDA catalyzer combines Au's surface plasmon resonance excitation with Pt-MOF Schottky junction, and exhibits extremely efficient photocatalytic H production under visible light irradiation. The [email protected]@QDs/PDA achieves the aggregation of rheumatoid synovial cells by the extravasation through "ELVIS" effect (extravasation through leaky vasculature and subsequent inflammatory cell-mediated sequestration) and extremely efficient photocatalytic H production. By combining PTT and H therapy, the [email protected]@QDs/PDA relieves the oxidative stress of RA, and shows significant improvement in joint damage and inhibition of the overall arthritis severity of collagen-induced RA mouse models. Therefore, the [email protected]@QDs/PDA shows great potential in the treatment of RA and further clinical transformation.
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http://dx.doi.org/10.1016/j.mtbio.2022.100214DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8850757PMC
January 2022

Circulating Collagen Metabolites and the Enhanced Liver Fibrosis (ELF) Score as Fibrosis Markers in Systemic Sclerosis.

Front Pharmacol 2022 1;13:805708. Epub 2022 Feb 1.

Department of Rheumatology, Huashan Hospital, Fudan University, Shanghai, China.

Serum fibrosis markers for systemic sclerosis (SSc) remain limited. The Enhanced Liver Fibrosis (ELF) score is a collagen marker set consisting of procollagen type III amino terminal propeptide (PIIINP), tissue inhibitor of metalloproteinases 1 (TIMP-1), and hyaluronic acid (HA). This longitudinal study aimed to examine the performance of the ELF score and its single analytes as surrogate outcome measures of fibrosis in SSc. Eighty-five SSc patients fulfilling the 2013 ACR/EULAR criteria with the absence of chronic liver diseases were enrolled. Serum PIIINP, TIMP-1, HA, and the ELF score were measured and correlated with clinical variables including the modified Rodnan skin score (mRSS) and interstitial lung disease (ILD). Twenty SSc patients underwent a follow-up serological testing and mRSS evaluation during treatment with immunosuppressants and/or anti-fibrotic drugs. Serum PIIINP, TIMP-1, and ELF score were significantly higher in patients with SSc than in healthy controls [PIIINP: 10.31 (7.83-14.10) vs. 5.61 (4.69-6.30), < .001; TIMP-1: 110.73 (66.21-192.45) vs. 61.81 (48.86-85.24), < .001; ELF: 10.34 (9.91-10.86) vs. 9.68 (9.38-9.99), < .001]. Even higher levels of PIIINP, TIMP-1, and ELF score were found in patients with diffuse cutaneous SSc than those with limited cutaneous SSc. At baseline, both PIIINP and ELF score showed good correlation with mRSS (PIIINP: r = .586, < .001; ELF: r = .482, < .001). Longitudinal analysis showed that change in PIIINP positively correlated with change in mRSS (r = 0.701, = .001), while change in ELF score were not related, in a statistical context, to the change in mRSS (ELF: r = .140, = .555). Serum TIMP-1 was significantly higher in SSc patients with ILD, compared to the matched group of patients without ILD [109.45 (93.05-200.09) vs. 65.50 (40.57-110.73), = 0.007]. In patients with SSc, the ELF score well correlates with the extent of skin fibrosis, while serum PIIINP is a sensitive marker for longitudinal changes of skin fibrosis. In the future, circulating collagen metabolites may potentially be used to evaluate therapeutic effects of anti-fibrotic treatments in the disease.
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http://dx.doi.org/10.3389/fphar.2022.805708DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8844460PMC
February 2022

A dual-mode strategy combining SERS with MALDI FTICR MS based on core-shell silver nanoparticles for dye identification and semi-quantification in unearthed silks from Tang Dynasty.

Talanta 2022 May 29;241:123277. Epub 2022 Jan 29.

Beijing National Laboratory for Molecular Sciences, CAS Research/Education Center for Excellence in Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Beijing Mass Spectrum Center, Institute of Chemistry Chinese Academy of Sciences, Beijing, China; Graduate School, University of Chinese Academy of Sciences, Beijing, China. Electronic address:

Silk, as one of the representative artifacts of China, profoundly affects the communication between eastern and western civilizations, and dyes, as the color support of silks, reflected crucial historical, cultural and technological information. Surface enhanced Raman spectroscopy (SERS) characterized by vibrational information has been extensively employed in dye analysis. However, since natural plants with complex coloring compositions in ancient China were broadly applied in dying textiles, the existing SERS methods often misinterpret results in dye analysis. Besides, semi-quantification of each component was of great difficulty by SERS, limiting the exquisite comparative analyses of different historical samples. For the first time, a dual-mode strategy combining SERS with high mass resolution MALDI FTICR MS was developed in virtue of core-shell silver nanoparticles ([email protected]), which realized the precise identification and semi-quantification of complex dye mixtures, thus significantly improving the accuracy and applicability of traditional SERS method. Four typical dye components (alizarin, purpurin, berberine and indigo) have been identified and semi-quantified in unearthed dyed silks from Tang Dynasty based on the method. More interestingly, multiple dye components with different contents and their ratio could be precisely determined, which might help in further investigating their dyeing techniques. This dual-mode strategy represents a promising tool for providing solid support for cognition, evaluation and restoration of textile objects in museums and conservation centers.
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http://dx.doi.org/10.1016/j.talanta.2022.123277DOI Listing
May 2022

Single-Vesicle Electrochemistry Reveals Sex Difference in Vesicular Storage and Release of Catecholamine.

Angew Chem Int Ed Engl 2022 03 16;61(14):e202117596. Epub 2022 Feb 16.

Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences (CAS), Beijing, 100190, China.

Quantitative measurements of sex difference in vesicle chemistry (i.e., chemical storage and release) at the single-vesicle level are essential to understand sex differences in cognitive behaviors; however, such measurements are very challenging to conventional analytical methods. By using single-vesicle electrochemistry, we find the duration of single exocytotic events of chromaffin cells prepared from male rats is statistically longer than that from female rats, leading to more neurotransmitter released in the male group. Further analysis reveals that a higher percentage of vesicles in the female group release part of the neurotransmitter, i.e., partial release, during exocytosis than that in male group. This sex dimorphism in neurotransmitter release in exocytosis might relate to the sex difference in the expression of voltage-dependent calcium channels and membrane lipid composition. Our finding offers the first experimental evidence that sex dimorphism even exists in vesicle chemistry, providing a brand new viewpoint for understanding the sex dimorphism in exocytosis.
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http://dx.doi.org/10.1002/anie.202117596DOI Listing
March 2022

Recent advancements in microfluidic chip biosensor detection of foodborne pathogenic bacteria: a review.

Anal Bioanal Chem 2022 Apr 21;414(9):2883-2902. Epub 2022 Jan 21.

College of Chemistry and Chemical Engineering, Xinjiang Normal University, Urumqi, 830017, China.

Foodborne diseases caused by pathogenic bacteria pose a serious threat to human health. Early and rapid detection of foodborne pathogens is an urgent task for preventing disease outbreaks. Microfluidic devices are simple, automatic, and portable miniaturized systems. Compared with traditional techniques, microfluidic devices have attracted much attention because of their high efficiency and convenience in the concentration and detection of foodborne pathogens. This article firstly reviews the bio-recognition elements integrated on microfluidic chips in recent years and the progress of microfluidic chip development for pathogen pretreatment. Furthermore, the research progress of microfluidic technology based on optical and electrochemical sensors for the detection of foodborne pathogenic bacteria is summarized and discussed. Finally, the future prospects for the application and challenges of microfluidic chips based on biosensors are presented.
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http://dx.doi.org/10.1007/s00216-021-03872-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8782221PMC
April 2022

Application progress of magnetic molecularly imprinted polymers chemical sensors in the detection of biomarkers.

Analyst 2022 Feb 14;147(4):571-586. Epub 2022 Feb 14.

College of Chemistry and Chemical Engineering, Xinjiang Normal University, Urumqi 830054, China.

Specific recognition and highly sensitive detection of biomarkers play an essential role in identification, early diagnosis and prevention of many diseases. Magnetic molecularly imprinted polymers (MMIPs) have been widely used to capture biomimetic receptors for targets in various complex matrices due to their superior recognition ability, structural stability, and rapid separation characteristics, which overcome the existing deficiencies of traditional recognition elements such as antibodies, aptamers. The integration of MMIPs as recognition elements with chemical sensors opens new opportunities for the development of advanced analytical devices with improved selectivity and sensitivity, shorter analysis time, and lower cost. Recently, MMIPs-chemical sensors (MMIPs-CS) have made significant progress in detection, but many challenges and development spaces remain. Therefore, this review focuses on the research progress of the sensor based on biomarker detection and introduces the surface modification of the magnetic support material used to prepare high selective MMIPs, as well as the selective extraction of target biomarkers by MMIPs from the complex biological sample matrix. Based on the understanding of optical sensors and electrochemical sensors, the applications of MMIPs-optical sensors (MMIPs-OS) and MMIPs-electrochemical sensors (MMIPs-ECS) for biomarker detection were reviewed and discussed in detail. Moreover, it provides an overview of the challenges in this research area and the potential strategies for the rational design of high-performance MMIPs-CS, accelerating the development of multifunctional MMIPs-CS.
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http://dx.doi.org/10.1039/d1an01112jDOI Listing
February 2022

SERS-based boronate affinity biosensor with biomimetic specificity and versatility: Surface-imprinted magnetic polymers as recognition elements to detect glycoproteins.

Anal Chim Acta 2022 Jan 17;1191:339289. Epub 2021 Nov 17.

College of Chemistry and Chemical Engineering, Xinjiang Normal University, Urumqi, 830054, China. Electronic address:

Glycoproteins are a class of proteins with significant biological functions and clinical implications. Due to glycoproteins' reliability for the quantitative analysis, they have been used as biomarkers and therapeutic targets for disease diagnosis. We propose a sandwich structure-based boronate affinity biosensor that can separate and detect target glycoproteins by magnetic separation and Surface-enhanced Raman scattering (SERS) probes. The biosensor relies on boronic acid affinity magnetic molecularly imprinted polymer (MMIPs) with pH response as "capturing probe" for glycoproteins, and [email protected] modified with 4-mercaptophenylboronic acid (MPBA) as SERS probes, among which, MPBA has both strong SERS activity and can specifically recognize and bind to glycoproteins. MMIPs ensured specific and rapid analysis, and SERS detection provided high sensitivity. The proposed boronate affinity SERS strategy exhibited universal applicability and provided high sensitivity with limit of detection of 0.053 ng/mL and 0.078 ng/mL for horseradish peroxidase and acid phosphatase, respectively. Ultimately, the boronate affinity SERS strategy was successfully applied in detection of glycoprotein in spiked serum sample with recovery between 90.6% and 103.4%, respectively. In addition, this study used a portable Raman meter, which can meet the requirements of point-of-care testing. The biosensor presented here also has advantages in terms of cost-effectiveness, stability, and detection speed.
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http://dx.doi.org/10.1016/j.aca.2021.339289DOI Listing
January 2022

Nanobody-Functionalized Cellulose for Capturing SARS-CoV-2.

Appl Environ Microbiol 2022 03 5;88(5):e0230321. Epub 2022 Jan 5.

Department of Bioengineering, Northeastern Universitygrid.261112.7, Boston, Massachusetts, USA.

The highly transmissible severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has infected more than 253 million people, claiming ∼5.1 million lives to date. Although mandatory quarantines, lockdowns, and vaccinations help curb viral transmission, there is a pressing need for cost-effective systems to mitigate the viral spread. Here, we present a generic strategy for capturing SARS-CoV-2 through functionalized cellulose materials. Specifically, we developed a bifunctional fusion protein consisting of a cellulose-binding domain and a nanobody (Nb) targeting the receptor-binding domain of SARS-CoV-2. The immobilization of the fusion proteins on cellulose substrates enhanced the capture efficiency of Nbs against SARS-CoV-2 pseudoviruses of the wild type and the D614G variant, the latter of which has been shown to confer higher infectivity. Furthermore, the fusion protein was integrated into a customizable chromatography with highly porous cellulose to capture viruses from complex fluids in a continuous fashion. By capturing and containing viruses through the Nb-functionalized cellulose, our work may find utilities in virus sampling and filtration through the development of paper-based diagnostics, environmental tracking of viral spread, and reducing the viral load from infected individuals. The ongoing efforts to address the COVID-19 pandemic center around the development of diagnostics, preventative measures, and therapeutic strategies. In comparison to existing work, we have provided a complementary strategy to capture SARS-CoV-2 by functionalized cellulose materials through paper-based diagnostics as well as virus filtration in perishable samples. Specifically, we developed a bifunctional fusion protein consisting of both a cellulose-binding domain and a nanobody specific for the receptor-binding domain of SARS-CoV-2. As a proof of concept, the fusion protein-coated cellulose substrates exhibited enhanced capture efficiency against SARS-CoV-2 pseudovirus of both the wild type and the D614G variant, the latter of which has been shown to confer higher infectivity. Furthermore, the fusion protein was integrated into a customizable chromatography for binding viruses from complex biological fluids in a highly continuous and cost-effective manner. Such antigen-specific capture can potentially immobilize viruses of interest for viral detection and removal, which contrasts with the common size- or affinity-based filtration devices that bind a broad range of bacteria, viruses, fungi, and cytokines present in blood (https://clinicaltrials.gov/ct2/show/NCT04413955). Additionally, since our work focuses on capturing and concentrating viruses from surfaces and fluids as a means to improve detection, it can serve as an "add-on" technology to complement existing viral detection methods, many of which have been largely focusing on improving intrinsic sensitivities.
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http://dx.doi.org/10.1128/aem.02303-21DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8904043PMC
March 2022

Associations of fruit & vegetable intake and physical activity with poor self-rated health among Chinese older adults.

Authors:
Ming Guan

BMC Geriatr 2022 01 3;22(1):10. Epub 2022 Jan 3.

Family Issues Center, Xuchang University, Road Bayi 88, Xuchang, Henan, China.

Background: Despite the existing literature highlights the central roles of sociodemographic factors, fruit & vegetable (F&V) intake, and physical activities for maintaining good health, less is known about the associations in the Chinese context. This study attempted to explore the associations of servings of F&V intake and levels of physical activities with poor self-rated health (SRH) among Chinese older adults.

Methods: Data were drawn from the Study on Global Ageing and Adult Health-China (SAGE-China) issued by the World Health Organization and included 7560 respondents aged ≥60 years in China. After screening out the potential confounding factors, multiple logistic regression models were adopted to explore the associations of sociodemographic factors, servings of F&V intake, and levels of physical activities with poor SRH.

Results: Among the sample, nearly a quarter reported poor health status. There were significant gender differences in the case of servings of F&V intake and levels of physical activities. Logistic regressions indicated that higher fruit intake was associated with lower likelihood of vigorous level of physical activity as compared to zero intake. Likewise, higher vegetable intake (≥10 servings) was associated with a higher likelihood of vigorous & moderate level of physical activity when compared to lower intake (≤ 4 servings). Higher fruit intake was associated with a lower likelihood of poor SRH. Similarly, vegetable intake (5 servings: AOR = 0.69, 95%CI: 0.58-0.83; 6-9 servings: AOR = 0.72, 95%CI: 0.59-0.87) was significantly associated with poor SRH. Additionally, vigorous level of physical activity (AOR = 0.79, 95%CI: 0.65-0.97) and vigorous fitness/leisure (AOR = 0.57, 95%CI: 0.39-0.84) were significantly associated with poor SRH.

Conclusion: This study suggested that older adults with high fruit intake had lower probability of performing vigorous & moderate level of physical activity, while those with high vegetable intake had higher probability of performing vigorous & moderate level of physical activity. Likewise, the older adults with high F&V intake and higher probability of performing vigorous level of physical activity, walk/bike activity, and vigorous/moderate fitness/leisure had less likelihood to face the risk for poor SRH outcomes. The appropriate servings of F&V intake and levels of physical activity should be highlighted.
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http://dx.doi.org/10.1186/s12877-021-02709-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8722069PMC
January 2022

Panel Associations Between Newly Dead, Healed, Recovered, and Confirmed Cases During COVID-19 Pandemic.

Authors:
Ming Guan

J Epidemiol Glob Health 2022 03 11;12(1):40-55. Epub 2021 Dec 11.

International Issues Center, Xuchang University, No. 88 Road Bayi, Xuchang, Henan, China.

Background: Currently, the knowledge of associations among newly recovered cases (NR), newly healed cases (NH), newly confirmed cases (NC), and newly dead cases (ND) can help to monitor, evaluate, predict, control, and curb the spreading of coronavirus disease 2019 (COVID-19). This study aimed to explore the panel associations of ND, NH, and NR with NC.

Methods: Data from China Data Lab in Harvard Dataverse with China (January 15, 2020 to January 14, 2021), the United States of America (the USA, January 21, 2020 to April 5, 2021), and the World (January 22, 2020 to March 20, 2021) had been analyzed. The main variables included in the present analysis were ND, NH, NR, and NC. Pooled regression, stacked within-transformed linear regression, quantile regression for panel data, random-effects negative binomial regression, and random-effects Poisson regression were conducted to reflect the associations of ND, NH, and NR with NC. Event study analyses were performed to explore how the key events influenced NC.

Results: Descriptive analyses showed that mean value of ND/NC ratio regarding China was more than those regarding the USA and the World. The results from tentative analysis reported the significant relationships among ND, NH, NR, and NC regarding China, the USA, and the World. Panel regressions confirmed associations of ND, NH, and NR with NC regarding China, the USA, and the World. Panel event study showed that key events influenced NC regarding USA and the World more greatly than that regarding China.

Conclusion: The findings in this study confirmed the panel associations of ND, NH, and NR with NC in the three datasets. The efficiencies of various control strategies of COVID-19 pandemic across the globe were compared by the regression outcomes. Future direction of research work could explore the influencing mechanisms of the panel associations.
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http://dx.doi.org/10.1007/s44197-021-00019-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8664669PMC
March 2022

Sexual and reproductive health knowledge, sexual attitudes, and sexual behaviour of university students: Findings of a Beijing-Based Survey in 2010-2011.

Authors:
Ming Guan

Arch Public Health 2021 Nov 29;79(1):215. Epub 2021 Nov 29.

International Issues Center, Xuchang University, Road Bayi 88, Xuchang, Henan, China.

Background: Although several studies have attempted investigating sex-related knowledge, attitudes, and practices among young people in China, deeper insights were still needed to further understand how this population could be supported to make healthy and safe sexual choices. Against this background, this study used a large set of secondary data to examine associations among sexual and reproductive health (SRH) knowledge, sexual attitudes, and sexual behaviour.

Methods: A cross-sectional study was employed to explore the associations among SRH knowledge, sexual attitudes, and sexual behaviour with a publicly available survey data among the 1196 university students from freshmen to Ph.D. candidates. Descriptive analysis was used to describe the sociodemographic characteristics of the university students by gender. Associations of sociodemographic factors with sources and categories of SRH knowledge, categories of contraceptive knowledge, sexual attitudes, and sexual behavior were explored with Poisson regressions and logistic regressions, respectively. The mediating effects of sociodemographic factors on the associations between SRH knowledge and sexual behavior, observed sexual attitudes on the associations of SRH knowledge with sexual behavior, and latent sexual attitudes on the relationship between SRH knowledge and sexual behavior were analyzed in order.

Results: Descriptive analysis showed that the sample was dominated by age group (18 to 24 years), undergraduates, females, limited contraceptive knowledge, unfavorable sexual attitudes, and insufficient knowledge sources. Regression analyses showed that sociodemographic factors had significant associations with SRH knowledge, sexual attitudes, and sexual behavior. Subsequently, the mediating effects of sociodemographic factors on the associations of SRH knowledge and sexual attitudes with sexual behavior were confirmed. Controlling for sociodemographic factors, the effects of sexual attitudes on the associations between SRH knowledge and sexual behaviour could be verified. Structural equation modeling indicated that the linear sequence of sources and categories of SRH knowledge → sexual attitudes → sexual behaviour model and the triangle mediating effects of sexual behaviour → sexual attitudes → SRH knowledge model existed.

Conclusions: Sociodemographic factors and observed sexual attitudes mediated the associations between SRH knowledge and sexual behaviour. The sequence relationship: sources and categories of SRH knowledge → sexual attitudes → sexual behavior and the mediating relationship: sexual behavior → sexual attitude → sources and categories of SRH knowledge & sexual behavior → sources and categories of SRH knowledge and sexual behavior → sexual attitude → categories of contraceptive knowledge & sexual behavior → categories of contraceptive knowledge were confirmed in the sample. This study also identified an urgent need for the university students to access to SRH comprehensive knowledge.
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http://dx.doi.org/10.1186/s13690-021-00739-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8628385PMC
November 2021

Detection of 2 Gene Methylation in Extramammary Paget's Disease by Methylation-Sensitive High-Resolution Melting Analysis.

J Oncol 2021 1;2021:5514426. Epub 2021 Nov 1.

Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical School, Fudan University, Shanghai, China.

Background: Extramammary Paget's disease (EMPD) is a rare skin tumor. Hypermethylation in the 2 promoter resulting in the downregulation of its protein expression shows a high detection rate in EMPD tumor tissue, which indicates that the methylation of 2 may play an important role in the pathogenesis of EMPD.

Objective: This study aims to establish a rapid analysis strategy based on the methylation-sensitive high-resolution melting curve (MS-HRM) to detect the methylation level of the 2 promoter.

Methods: With the use of universal methylated human DNA products, we established the MS-HRM standard curve to quantitatively detect the methylation level of the 2 promoter. Then, all 57 EMPD tumor DNA samples were analyzed. Pyrosequencing assay was also carried out to test the accuracy and efficacy of MS-HRM. Besides, a total of 54 human normal and other cancerous tissues were included in this study to test the reliability and versatility of the MS-HRM standard curve.

Results: In this study, by using the established MS-HRM, we found that 96.5% (55/57) EMPD tumor samples had varying methylation levels in the 2 promoter ranging from 0% to 30%. Then, the methylation data were compared to the results obtained from pyrosequencing, which showed a high correlation between these two techniques by Pearson's correlation ( = 0.9425) and Bland-Altman plots (mean difference = -0.1069) indicating that the methylation levels analyzed by MS-HRM were consistent with DNA pyrosequencing. Furthermore, in 23 normal and 31 other cancerous tissue samples, there were two colorectal cancer (CRC) tissues that tested 2 methylation positive (1% and 5%) which confirmed that our established MS-HRM can be widely applied to various types of samples.

Conclusion: MS-HRM standard curve can be used for the detection of the methylation level of 2 in EMPD tumor samples and other cancerous tissues potentially, which presents a promising candidate as a quantitative assay to analyze 2 promoter methylation in routine pathological procedure.
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http://dx.doi.org/10.1155/2021/5514426DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8575627PMC
November 2021

Evaluation of analytic and clinical performance of two immunoassays for detecting thyroid-stimulating receptor antibody in the diagnosis of Graves' disease.

J Clin Lab Anal 2021 Dec 9;35(12):e23950. Epub 2021 Nov 9.

Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, China.

Objective: To evaluate the analytical and clinical performance of two immunoassays for diagnosis of Graves' disease (GD), the Immulite thyroid-stimulating immunoglobulin (TSI), and Elecsys Anti-TSH receptor (TSHR) assay.

Methods: Precision and analytical measurement range were assessed using pooled samples of patients. The comparison between the two methods was evaluated using 579 clinical samples, and receiver operating characteristic (ROC) curves were drawn using the final diagnosis as reference. Clinical sensitivity and specificity, accuracy, positive predictive value (PPV), and negative predictive value (NPV) were calculated for the two tests.

Results: The repeatability and intermediate imprecision coefficient of variation (CV%) of the TSI assay were 3.8% and 4.1% at 0.95 IU/L, and 3.5% and3.6% at 19.5 IU/L, respectively. The assays were linear over a range 0.27-38.5 IU/L. There was a high correlation between the quantitative results of the two methods (correlation coefficient r = 0.930). The cut-off value obtained by ROC analysis for TSI assay was 0.7 IU/L with sensitivity of 93.7% and specificity of 85.1%. An overall qualitative agreement of 91.5% between two methods was observed. Among 44 patients with discordant qualitative results, the TSI assay provided more satisfactory results consistent with clinical diagnoses.

Conclusion: The TSI assay showed excellent analytical performance and provided a high PPV for GD.
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http://dx.doi.org/10.1002/jcla.23950DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8649345PMC
December 2021

Quality Management for Point-Of-Care Testing of Pathogen Nucleic Acids: Chinese Expert Consensus.

Front Cell Infect Microbiol 2021 13;11:755508. Epub 2021 Oct 13.

Department of Laboratory Medicine, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.

COVID-19 continues to circulate globally in 2021, while under the precise policy implementation of China's public health system, the epidemic was quickly controlled, and society and the economy have recovered. During the pandemic response, nucleic acid detection of SARS-CoV-2 has played an indispensable role in the first line of defence. In the cases of emergency operations or patients presenting at fever clinics, nucleic acid detection is required to be performed and reported quickly. Therefore, nucleic acid point-of-care testing (POCT) technology for SARS-CoV-2 identification has emerged, and has been widely carried out at all levels of medical institutions. SARS-CoV-2 POCT has served as a complementary test to conventional polymerase chain reaction (PCR) batch tests, thus forming an experimental diagnosis platform that not only guarantees medical safety but also improves quality services. However, in view of the complexity of molecular diagnosis and the biosafety requirements involved, pathogen nucleic acid POCT is different from traditional blood-based physical and chemical index detection. No guidelines currently exist for POCT quality management, and there have been inconsistencies documented in practical operation. Therefore, Shanghai Society of Molecular Diagnostics, Shanghai Society of Laboratory Medicine, Clinical Microbiology Division of Shanghai Society of Microbiology and Shanghai Center for Clinical Laboratory have cooperated with experts in laboratory medicine to generate the present expert consensus. Based on the current spectrum of major infectious diseases in China, the whole-process operation management of pathogen POCT, including its application scenarios, biosafety management, personnel qualification, performance verification, quality control, and result reporting, are described here. This expert consensus will aid in promoting the rational application and robust development of this technology in public health defence and hospital infection management.
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http://dx.doi.org/10.3389/fcimb.2021.755508DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8548827PMC
November 2021

Stratified Disk Microrobots with Dynamic Maneuverability and Proton-Activatable Luminescence for Imaging.

ACS Nano 2021 12 29;15(12):19924-19937. Epub 2021 Oct 29.

Institute for Biomedical Materials & Devices, Faculty of Science, The University of Technology Sydney, Ultimo, New South Wales 2007, Australia.

Microrobots can expand our abilities to access remote, confined, and enclosed spaces. Their potential applications inside our body are obvious, .., to diagnose diseases, deliver medicine, and monitor treatment efficacy. However, critical requirements exist in relation to their operations in gastrointestinal environments, including resistance to strong gastric acid, responsivity to a narrow proton variation window, and locomotion in confined cavities with hierarchical terrains. Here, we report a proton-activatable microrobot to enable real-time, repeated, and site-selective pH sensing and monitoring in physiological relevant environments. This is achieved by stratifying a hydrogel disk to combine a range of functional nanomaterials, including proton-responsive molecular switches, upconversion nanoparticles, and near-infrared (NIR) emitters. By leveraging the 3D magnetic gradient fields and the anisotropic composition, the microrobot can be steered to locomote as a gyrating "Euler's disk", .., aslant relative to the surface and along its low-friction outer circumference, exhibiting a high motility of up to 60 body lengths/s. The enhanced magnetomotility can boost the pH-sensing kinetics by 2-fold. The fluorescence of the molecular switch can respond to pH variations with over 600-fold enhancement when the pH decreases from 8 to 1, and the integration of upconversion nanoparticles further allows both the efficient sensitization of NIR light through deep tissue and energy transfer to activate the pH probes. Moreover, the embedded down-shifting NIR emitters provide sufficient contrast for imaging of a single microrobot inside a live mouse. This work suggests great potential in developing multifunctional microrobots to perform generic site-selective tasks .
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http://dx.doi.org/10.1021/acsnano.1c07431DOI Listing
December 2021
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