Publications by authors named "Ming Gao"

881 Publications

A novel lncRNA, RPL34-AS1, promotes proliferation and angiogenesis in glioma by regulating VEGFA.

J Cancer 2021 27;12(20):6189-6197. Epub 2021 Aug 27.

Department of Neurosurgery, The First Affiliated Hospital of Harbin Medical University, Harbin, China.

Brain gliomas are the most common primary malignant tumors of the central nervous system and one of the leading causes of death in patients with intracranial tumors. The lncRNA RPL34-AS1 is significantly upregulated in glioma tissues. However, the biological function of RPL34-AS1, especially in proliferation in glioma, remains unclear. The role of RPL34-AS1 in proliferation and angiogenesis in glioma cells was investigated using the LN229, U87, and U251 glioma cell lines. The levels of RPL34-AS1 were detected using real-time quantitative reverse transcription polymerase chain reaction. CCK-8 and colony formation assays were performed to determine the role of RPL34-AS1 in proliferation and survival, and its role in angiogenesis was assessed by an endothelial tube formation assay. Changes in protein levels were assessed by western blotting. RPL34-AS1 was upregulated in glioma tissues and was correlated with tumor grade. RPL34-AS1 expression was also higher in glioma cells than in normal astrocytes. Knockdown of RPL34-AS1 blocked glioma cell proliferation by inhibiting angiogenesis. This effect occurred through decreased ERK/AKT signaling. This study suggests that RPL34-AS1 affects cell proliferation and angiogenesis in glioma and therefore may potentially serve as a valuable diagnostic and prognostic biomarker and therapeutic target in patients with glioma.
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http://dx.doi.org/10.7150/jca.59337DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8425216PMC
August 2021

Germline and sporadic mTOR pathway mutations in low-grade oncocytic tumor of the kidney.

Mod Pathol 2021 Sep 20. Epub 2021 Sep 20.

Kidney Cancer Program at Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, Dallas, TX, USA.

Low-grade oncocytic tumor (LOT) of the kidney is a recently described entity with poorly understood pathogenesis. Using next-generation sequencing (NGS) and complementary approaches, we provide insight into its biology. We describe 22 LOT corresponding to 7 patients presenting with a median age of 75 years (range 63-86 years) and male to female ratio 2:5. All 22 tumors demonstrated prototypical microscopic features. Tumors were well-circumscribed and solid. They were composed of sheets of tumor cells in compact nests. Tumor cells had eosinophilic cytoplasm, round to oval nuclei (without nuclear membrane irregularities), focal subtle perinuclear halos, and occasional binucleation. Sharply delineated edematous stromal islands were often observed. Tumor cells were positive for PAX8, negative for CD117, and exhibited diffuse and strong cytokeratin-7 expression. Six patients presented with pT1 tumors. At a median follow-up of 29 months, four patients were alive without recurrence (three patients had died from unrelated causes). All tumors were originally classified as chromophobe renal cell carcinoma, eosinophilic variant (chRCC-eo). While none of the patients presented with known syndromic features, one patient with multiple bilateral LOTs was subsequently found to have a likely pathogenic germline TSC1 mutation. Somatic, likely activating, mutations in MTOR and RHEB were identified in all other evaluable LOTs. As assessed by phospho-S6 and phospho-4E-BP1, mTOR complex 1 (mTORC1) was activated across all cases but to different extent. MTOR mutant LOT exhibited lower levels of mTORC1 activation, possibly related to mTORC1 dimerization and the preservation of a wild-type MTOR copy (retained chromosome 1). Supporting its distinction from related entities, gene expression analyses showed that LOT clustered separately from classic chRCC, chRCC-eo, and RO. In summary, converging mTORC1 pathway mutations, mTORC1 complex activation, and a distinctive gene expression signature along with characteristic phenotypic features support LOT designation as a distinct entity with both syndromic and non-syndromic cases associated with an indolent course.
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http://dx.doi.org/10.1038/s41379-021-00896-6DOI Listing
September 2021

Bamlanivimab improves hospitalization and mortality rates in patients with COVID-19: A systematic review and meta-analysis.

J Infect 2021 Sep 14. Epub 2021 Sep 14.

Department of Neurology, the Affiliated Hospital of Southwest Jiaotong University & the Third People's Hospital of Chengdu, Sichuan, China. Electronic address:

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http://dx.doi.org/10.1016/j.jinf.2021.09.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8438996PMC
September 2021

L-citrulline inhibits body weight gain and hepatic fat accumulation by improving lipid metabolism in a rat nonalcoholic fatty liver disease model.

Food Sci Nutr 2021 Sep 16;9(9):4893-4904. Epub 2021 Jul 16.

School of Pharmaceutical Science Mukogawa Women's University Nishinomiya Japan.

Background: Body weight gain is a social issue all over the world. When body weight increased, hepatic fat accumulation also increased and it causes fatty liver disease. Therefore, developing a new treatment method and elucidating its mechanism is necessary. L-citrulline (L-Cit) is a free amino acid found mainly in watermelon. No reports regarding its effects on the improvement of hepatic steatosis and fibrogenesis are currently available. The aim of this study was to clarify the effect and the mechanism of L-Cit on inhibition of body weight gain and hepatic fat accumulation in high-fat and high-cholesterol fed SHRSP5/Dmcr rats.

Methods: L-Cit or water (controls) was administered to six-week-old male SHRSP5/Dmcr rats by gavage for nine weeks. We recorded the level of body weight and food intake while performing the administration and sacrificed rats. After that, the blood and lipid metabolism-related organs and tissues were collected and analyzed.

Results: L-Cit treatment reduced body weight gain and hepatic TC and TG levels, and serum levels of AST and ALT. L-Cit enhanced AMPK, LKB1, PKA, and hormone-sensitive lipase (HSL) protein phosphorylation levels in the epididymal fat. L-Cit treatment improved steatosis as revealed by HE staining of liver tissues and enhanced AMPK and LKB1 phosphorylation levels. Moreover, activation of Sirt1 was higher, while the liver fatty acid synthase (FAS) level was lower. Azan staining of liver sections revealed a reduction in fibrogenesis following L-Cit treatment. Further, the liver levels of TGF-β, Smad2/3, and α-SMA, fibrogenesis-related proteins and genes, were lower in the L-Cit-treated group.

Conclusions: From the results of analysis of the epididymal fat and the liver, L-Cit inhibits body weight gain and hepatic fat accumulation by activating lipid metabolism and promoting fatty acid β-oxidation in SHRSP5/Dmcr rats.
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http://dx.doi.org/10.1002/fsn3.2439DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8441368PMC
September 2021

Circ-SFMBT2 drives the malignant phenotypes of esophageal cancer by the miR-107-dependent regulation of SLC1A5.

Cancer Cell Int 2021 Sep 16;21(1):495. Epub 2021 Sep 16.

Department of Oncology, The First Affiliated Hospital of Zhengzhou University, No.1 Jianshe East Road, Erqi District, Zhengzhou, Henan, 450052, People's Republic of China.

Background: Increasing studies focused on the regulatory roles of circular RNAs (circRNAs) in diverse cancers. This study was to evaluate the function and mechanism of circRNA Scm-like with four malignant brain tumor domains 2 (circ-SFMBT2) in esophageal cancer (EC).

Methods: The circ-SFMBT2, microRNA-107 (miR-107) and solute-linked carrier family A1 member 5 (SLC1A5) levels were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR). Cell proliferation was evaluated by 3-(4, 5-dimethylthiazol-2-y1)-2, 5-diphenyl tetrazolium bromide (MTT) assay, colony formation assay and EdU assay. Cell apoptosis and invasion were detected by flow cytometry and transwell assay. Glutamine metabolism was assessed by the corresponding kits for glutamine consumption, α-ketoglutarate production and glutamate production. Western blot was used for protein quantification. The binding analysis was performed using dual-luciferase reporter assay, RNA immunoprecipitation (RIP) and pull-down assays. The functional research of circ-SFMBT2 in vivo was performed by xenograft tumor assay. Exosomes were identified by morphological observation and protein detection.

Results: Circ-SFMBT2 was overexpressed in EC samples and cells. Circ-SFMBT2 downregulation inhibited EC cell proliferation, invasion and glutamine metabolism. Circ-SFMBT2 targeted miR-107 and the regulation of circ-SFMBT2 was achieved by sponging miR-107. SLC1A5 was a target of miR-107, and it worked as an oncogene in EC cells. MiR-107 retarded the EC progression by downregulating SLC1A5. Circ-SFMBT2 could affect the SLC1A5 expression by targeting miR-107. Circ-SFMBT2 regulated EC progression in vivo by miR-107/SLC1A5 axis. Circ-SFMBT2 was transferred by exosomes in EC cells.

Conclusion: These results suggested that circ-SFMBT2 upregulated the SLC1A5 expression to promote the malignant development of EC by serving as a miR-107 sponge.
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http://dx.doi.org/10.1186/s12935-021-02156-8DOI Listing
September 2021

Nanosensing Colon Cancer Biomarker on Zeolite-modified Gap-fingered Dielectrodes.

Biotechnol Appl Biochem 2021 Sep 15. Epub 2021 Sep 15.

Faculty of Chemical Engineering Technology.

Nanomaterial on the sensing area elevates the biomolecular immobilization by its right orientation with a proper alignment and zeolite is one of the suitable materials. In this research zeolite nanoparticle was synthesized using rice hush ash as the basic source and the prepared zeolite by the addition of sodium silicate was utilized to attach antibody as a probe on a gap-fingered dielectrode surface to identify the colon cancer biomarker, 'colon cancer secreted protein-2' (CCSP-2). FESEM and FETEM images confirmed the size of nanoparticle to be ∼15 nm and the occurrence of silica and alumina. Zeolite was modified on the electrode surface through the amine linker and then anti-CCSP-2 was attached by an aldehyde linker. On this surface, CCSP-2 was detected and attained the detection limit to be 3 nM on the linear regression curve with 3 to 5 nM of CCSP-2. Estimated by the determination co-efficient of y = 2.3952x - 4.4869 and R = 9041 with 3δ (n = 3). In addition, control proteins did not produce the notable current response representing the specific sensing of CCSP-2. This research is suitable to identify CCSP-2 at lower level in the blood-stream under the physiological condition of colon cancer patient. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/bab.2254DOI Listing
September 2021

Research trend analysis of composting based on Web of Science database.

Environ Sci Pollut Res Int 2021 Sep 9. Epub 2021 Sep 9.

School of Energy and Environmental Engineering, University of Science and Technology Beijing, Beijing, 100083, People's Republic of China.

Bibliometric analysis was used in this study for the quantitative evaluation of current research trends on composting. The research articles indexed from the Science Citation Index-Expanded in Web of Science database published from 2000 to 2019 were investigated. The USA, China and Spain were the top three countries considering the number of papers. Amongst the research institutes, CSIC of Spain, Chinese Academy of Sciences and Agriculture & Agri-Food Canada ranked the top three in total publication amount. Journals that published a significant number of literature regarding topics of composting included Environmental Sciences & Ecology, Agriculture and Engineering. In terms of research content, keywords such as heavy metal, heavy metal and biodegradation appeared frequently. In addition, the analysis of keywords revealed the following research hotspots in future studies: investigation of heavy metal passivator, optimisation of composting conditioner, development of all kinds of microorganisms, rational management of the composting process and improvement of solid waste life cycle assessment. To some extent, it helps to understand the current global status and trends of the related research.
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http://dx.doi.org/10.1007/s11356-021-16377-xDOI Listing
September 2021

Bifunctional two-dimensional copper-aluminum modified filter paper composite for efficient tetracycline removal: Synergy of adsorption and reusability by degradation.

Chemosphere 2021 Sep 2;287(Pt 2):132031. Epub 2021 Sep 2.

School of Chemistry and Chemical Engineering/Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, Shihezi University, Shihezi, China. Electronic address:

Herein, bifunctional two-dimensional copper-aluminum modified filter paper composite (2D-Cu/Al-C) was successfully prepared by simple calcination and showed ultrahigh adsorption performance and degradation potential. The adsorption removal of TC on 2D-Cu/Al-C all exceeded 92.2% under solution conditions of 10-200 mg/L TC, 100 mg/L 2D-Cu/Al-C, pH 8 and 298 K. The pseudo-second-order kinetic and Langmuir models better fitted the kinetic and isotherm data via spontaneous and exothermic process, and the maximum capacity of the 2D-Cu/Al-C was 2391.78 mg/g. Additionally, 2D-Cu/Al-C showed desired specific adsorption for TC (TC: 98.7%, norfloxacin: 5.8%, sulfamethoxazole: 2.1%, and ciprofloxacin: 1.8%) and it could effectively adsorbed TC even in the binary system (various coexisting ions or natural organic matter). After TC adsorbed on adsorbent was mineralized into CO and HO by adding peroxydisulfate to generate high electrode potential radical in another limited systems, the 2D-Cu/Al-C still had ∼89.12% on TC removal (initial concentration of 50 mg/L) after five experimental cycles. Zeta potential, FT-IR and XPS results indicated that the multi-adsorption mechanism, including electrostatic interactions, complexation, and H-bonds, played a vital role in the fast and efficient adsorption process. Thus, the way of combining adsorption and regeneration via degradation are green, non-polluting strategy which are expected to be applied for water purification in future environmental remediation.
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http://dx.doi.org/10.1016/j.chemosphere.2021.132031DOI Listing
September 2021

Improved diagnosis of thyroid cancer aided with deep learning applied to sonographic text reports: a retrospective, multi-cohort, diagnostic study.

Cancer Biol Med 2021 Sep 7. Epub 2021 Sep 7.

Department of Maxillofacial and Otorhinolaryngology Oncology, National Clinical Research Center for Cancer, Key Laboratory of Molecular Cancer Epidemiology of Tianjin, Key Laboratory of Cancer Prevention and Therapy of Tianjin, Tianjin Medical University Cancer Institute and Hospital, Tianjin Medical University, Tianjin 300060, China.

Objective: Large volume radiological text data have been accumulated since the incorporation of electronic health record (EHR) systems in clinical practice. We aimed to determine whether deep natural language processing algorithms could aid radiologists in improving thyroid cancer diagnosis.

Methods: Sonographic EHR data were obtained from the EHR database. Pathological reports were used as the gold standard for diagnosing thyroid cancer. We developed thyroid cancer diagnosis based on natural language processing (THCaDxNLP) to interpret unstructured sonographic text reports for thyroid cancer diagnosis. We used the area under the receiver operating characteristic curve (AUROC) as the primary metric to measure the performance of the THCaDxNLP. We compared the performance of thyroid ultrasound radiologists aided with THCaDxNLP those without THCaDxNLP using 5 independent test sets.

Results: We obtained a total number of 788,129 sonographic radiological reports. The number of thyroid sonographic data points was 132,277, 18,400 of which were thyroid cancer patients. Among the 5 test sets, the numbers of patients per set were 439, 186, 82, 343, and 171. THCaDxNLP achieved high performance in identifying thyroid cancer patients (the AUROC ranged from 0.857-0.932). Thyroid ultrasound radiologists aided with THCaDxNLP achieved significantly higher performances than those without THCaDxNLP in terms of accuracy (93.8% 87.2%; one-sided -test, adjusted = 0.003), precision (92.5% 86.0%; = 0.018), and F1 metric (94.2% 86.4%; = 0.007).

Conclusions: THCaDxNLP achieved a high AUROC for the identification of thyroid cancer, and improved the accuracy, sensitivity, and precision of thyroid ultrasound radiologists. This warrants further investigation of THCaDxNLP in prospective clinical trials.
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http://dx.doi.org/10.20892/j.issn.2095-3941.2020.0509DOI Listing
September 2021

Epidemiology and factors predicting survival of amyotrophic lateral sclerosis in a large Chinese cohort.

Chin Med J (Engl) 2021 Sep 2. Epub 2021 Sep 2.

Institute of Medical Innovation and Research, Peking University Third Hospital, Beijing 100191, China Department of Neurology, Peking University Third Hospital, Beijing 100191, China Department of Neurology, Affiliated Hospital of Weifang Medical University, Weifang, Shandong 261000, China Department of Anesthesiology, Fuwai Central China Cardiovascular Hospital, Zhengzhou, Henan 450000, China.

Background: Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disorder associated with loss of motor neurons. Our objective was to explore the epidemiology, clinical features, and survival factors of 1809 patients with ALS.

Methods: We analyzed 1809 ALS patients, who were recruited from the Peking University Third Hospital from January 2005 to December 2015. Demographic data and disease-related parameters were collected. Kaplan-Meier curves were used to compare survival time. Cox proportional hazards function and the hazard ratio were used to identify adjusted prognostic predictors.

Results: The results showed that the average annual incidence in Beijing alone was 0.38 cases/100,000 person-years and the mean age of onset was 48.88 ± 11.35 (95% confidence interval [CI]: 48.17-49.85) years. The median survival time from onset to death/tracheostomy was 58.89 ± 33.03 (95% CI: 51.46-63.84) months. In the adjusted Cox proportional hazard model, age of onset, diagnosis delay, rate of disease progression (Amyotrophic Lateral Sclerosis Functional Rating Scale Revised decline [points/month]), and body mass index all had an independent effect on survival in ALS.

Conclusions: Our study provides information on epidemiology, clinical features, and survival factors of patients with ALS in China. These results can be helpful in clinical practice, clinical trial design, and validation of new tools to predict disease progression.
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http://dx.doi.org/10.1097/CM9.0000000000001679DOI Listing
September 2021

Mesophilic condition is more conducive to methane production yield and tylosin removal on tylosin fermentation dreg anaerobic digestion.

Bioresour Technol 2021 Aug 19;341:125806. Epub 2021 Aug 19.

Department of Environmental Engineering, School of Energy and Environmental Engineering, University of Science and Technology Beijing, Beijing 100083, PR China; Beijing Key Laboratory on Disposal and Resource Recovery of Industry Typical Pollutants, University of Science and Technology Beijing, Beijing 100083, PR China.

Anaerobic digestion (AD) of antibiotic fermentation dreg is effective because of its satisfactory methanogenic performance and antibiotic removal rate. Thermophilic AD, an important branch, has not been studied. This study explored the effects of mesophilic and thermophilic AD on tylosin fermentation dreg (TFD) alone-digestion and co-digestion with food waste. The methane production (245-420 mL/g-VS) of mesophilic AD was 20.7%-28.6% higher than thermophilic AD, but the maximum methane production rate (R), lag period (λ), and hydrolysis rate constant (k) of thermophilic AD were 1.81, 0.236 and 3.16 times higher than those of mesophilic AD, respectively. However, the residual tylosin in mesophilic AD was always higher than that in thermophilic AD. The addition of food waste increased the methane production rate and yield under both temperature conditions. Therefore, due to the higher rate and yield of methane production, mesophilic AD, especially co-digestion, is more suitable for the treatment of TFD.
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http://dx.doi.org/10.1016/j.biortech.2021.125806DOI Listing
August 2021

MiRNA-34c Regulates Bovine Sertoli Cell Proliferation, Gene Expression, and Apoptosis by Targeting the Gene.

Animals (Basel) 2021 Aug 13;11(8). Epub 2021 Aug 13.

College of Animal Science, Jilin University, Changchun 130062, China.

MicroRNAs (miRNAs) play significant roles in mammalian spermatogenesis. Sertoli cells can provide a stable microenvironment and nutritional factors for germ cells, thus playing a vital role in spermatogenesis. However, few studies elucidate the regulation of bovine testicular Sertoli cells by miRNAs. Here, we have reported that miRNA-34c (miR-34c) regulates proliferation, apoptosis, and relative transcripts abundance gene in bovine Sertoli cells. In bovine Sertoli cells, overexpression of miR-34c inhibited proliferation and relative abundance of gene transcripts while promoting apoptosis of Sertoli cells, and the effects were the opposite when miR-34c was knocked down. Receptor tyrosine kinase () was identified as a direct target gene of miR-34c in Sertoli cells, validated by analysis of the relative abundance of transcript and dual-luciferase reporter assay. The relative abundance of the transcript of genes related to male reproduction in Sertoli cells was changed after the gene was overexpressed, as demonstrated by the RT2 Profiler PCR Array results. In summary, miR-34c specifically regulated the gene by targeting a sequence in the 3'-UTR, which could influence proliferation, apoptosis, and relative abundance of the transcript of male reproduction-related genes. Therefore, miR-34c could be considered an essential regulator in the process of bull spermatogenesis.
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http://dx.doi.org/10.3390/ani11082393DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8388803PMC
August 2021

A review of root exudates and rhizosphere microbiome for crop production.

Environ Sci Pollut Res Int 2021 Aug 24. Epub 2021 Aug 24.

Department of Environmental Engineering, School of Energy and Environmental Engineering, University of Science and Technology Beijing, Beijing, 100083, China.

Increasing crop yields and ensuring food security is a major global challenge. In order to increase crop production, chemical fertilizers and pesticides are excessively used. However, the significance of root exudates is understudied. Beneficial interactions between plant and rhizosphere microbiome are critical for plant fitness and health. In this review, we discuss the application and progress of current research methods and technologies in terms of root exudates and rhizosphere microbiome. We summarize how root exudates promote plant access to nitrogen, phosphorus, and iron, and how root exudates strengthen plant immunity to cope with biotic stress by regulating the rhizosphere microbiome, and thereby reducing dependence on fertilizers and pesticides. Optimizing these interactions to increase plant nutrient uptake and resistance to biotic stresses offers one of the few untapped opportunities to confront sustainability issues in food security. To overcome the limitations of current research, combination of multi-omics, imaging technology together with synthetic communities has the potential to uncover the interaction mechanisms and to fill the knowledge gap for their applications in agriculture to achieve sustainable development.
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http://dx.doi.org/10.1007/s11356-021-15838-7DOI Listing
August 2021

Characterization of the Human Oropharyngeal Microbiomes in SARS-CoV-2 Infection and Recovery Patients.

Adv Sci (Weinh) 2021 Aug 22:e2102785. Epub 2021 Aug 22.

Gene Hospital of Henan Province; Precision Medicine Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China.

Respiratory tract microbiome is closely related to respiratory tract infections, while characterization of oropharyngeal microbiome in recovered coronavirus disease 2019 (COVID-19) patients is not studied. Herein, oropharyngeal swabs are collected from confirmed cases (CCs) with COVID-19 (73 subjects), suspected cases (SCs) (36), confirmed cases who recovered (21), suspected cases who recovered (36), and healthy controls (Hs) (140) and then completed MiSeq sequencing. Oropharyngeal microbial α-diversity is markedly reduced in CCs versus Hs. Opportunistic pathogens are increased, while butyrate-producing genera are decreased in CCs versus Hs. The classifier based on eight optimal microbial markers is constructed through a random forest model and reached great diagnostic efficacy in both discovery and validation cohorts. Notably, the classifier successfully diagnosed SCs with positive IgG antibody as CCs and is demonstrated from the perspective of the microbiome. Importantly, several genera with significant differences gradually increase and decrease along with recovery from COVID-19. Forty-four oropharyngeal operational taxonomy units (OTUs) are closely correlated with 11 clinical indicators of SARS-CoV-2 infection and Hs based on Spearman correlation analysis. Together, this research is the first to characterize oropharyngeal microbiota in recovered COVID-19 cases and suspected cases, to successfully construct and validate the diagnostic model for COVID-19 and to depict the correlations between microbial OTUs and clinical indicators.
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http://dx.doi.org/10.1002/advs.202102785DOI Listing
August 2021

Mechanistic revealing of reproductive behavior impairment in male guppy (Poecilia reticulata) induced by environmentally realistic 2,2'-dithiobis-pyridine exposure.

Chemosphere 2021 Aug 12;286(Pt 3):131839. Epub 2021 Aug 12.

Colleges of Marine Life Sciences, Ocean University of China, Qingdao, 266003, Shandong province, China.

Although (PS), the primary degradation product of emerging antifouling biocides metal pyrithiones (MePTs), can disrupt the reproductive behavior of fish at an environmentally relevant ng/L level, the underlying mechanism is still largely unknown. This study exposed sexually mature male guppy (Poecilia reticulata) to 20, 200, and 2000 ng/L (PS) to explore the compromised effect of (PS) on reproductive behavior through a realistic competing scenario. The results showed that (PS) suppressed male guppies' sexual interest to stimulus females, reduced their competitive behavior frequencies toward rival males, and decreased their mating time and frequency. (PS) exposure did not affect male guppies' secondary sexual characteristics or induce estrogenic activity. Whole-brain transcriptome sequencing identified 1070 differentially expressed genes (DEGs) with 872 up-regulated genes, which were functionally enriched into Gene Ontology terms pertaining to extracellular matrix (ECM) and extracellular region. KEGG enrichment for the DEGs uncovered that the activations of ECM-receptor interaction and focal adhesion pathways could be the underlying molecular mechanism implicated in the (PS) induced reproductive behavior impairment. This work would deliver a substantial contribution to the understanding of the ecological safety of MePTs biocides.
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http://dx.doi.org/10.1016/j.chemosphere.2021.131839DOI Listing
August 2021

Hydroxychloroquine/chloroquine in patients with COVID-19 in Wuhan, China: a retrospective cohort study.

BMC Infect Dis 2021 Aug 12;21(1):805. Epub 2021 Aug 12.

Department of Rheumatology, Beijing Hospital, National Center of Gerontology, Beijing, China.

Background: Since the COVID-19 pandemic, several therapeutic agents have been used in COVID-19 management. However, the results were controversial. Here, we aimed to evaluate the efficacy and safety of hydroxychloroquine (HCQ)/chloroquine (CQ) in COVID-19.

Methods: We retrospectively reviewed the medical charts of patients with COVID-19 admitted to an inpatient ward in Wuhan from 2020/Feb/08 to 2020/Mar/05. Patients with HCQ/CQ and age, gender, disease severity matched ones without HCQ/CQ were selected at a 1:2 ratio. The clinical, laboratory and imaging findings were compared between these two groups. The multivariate linear regression analysis was performed to identify the factors that might influence patients' virus shedding periods (VSPs).

Results: A total of 14 patients with HCQ/CQ and 21 matched ones were analyzed. The HCQ/CQ treatment lasted for an average of 10.36 ± 3.12 days. The mean VSPs were longer in the HCQ/CQ treatment group (26.57 ± 10.35 days vs. 19.10 ± 7.80 days, P = 0.020). There were 3 patients deceased during inpatient period, two patients were with HCQ/CQ treatment (P = 0.551). In the multivariate linear regression analysis, disease durations at admission (t = 3.643, P = 0.001) and HCQ/CQ treatment (t = 2.637, P = 0.013) were independent parameters for patients' VSPs. One patient with CQ had recurrent first-degree atrioventricular block (AVB) and obvious QTc elongation, another one complained about dizziness and blurred vision which disappeared after CQ discontinuation. One patient with HCQ had transient AVB.

Conclusions: In summary, we identify that the HCQ/CQ administration is not related to less mortality cases at later phase of COVID-19. More studies are needed to explore whether HCQ/CQ treatment would lead to SARS-Cov-2 RNA clearance delay or not.
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http://dx.doi.org/10.1186/s12879-021-06477-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8358550PMC
August 2021

Carbazate-modified cross-linked dextran microparticles suppress the progression of osteoarthritis by ROS scavenging.

Biomater Sci 2021 Sep 14;9(18):6236-6250. Epub 2021 Sep 14.

Guangxi Collaborative Innovation Center for Biomedicine, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China.

A series of modified polysaccharide microparticles have been fabricated and their potential application for scavenging reactive oxygen species (ROS) and their derivatives to achieve osteoarthritis (OA) treatment has been explored. These microparticles were cross-linked dextran (Sephadex) with different carbazate substitution ratios determined by the TNBS assay and elemental analysis. It has been demonstrated that they could effectively scavenge carbonylated proteins and ROS including hydroxyl radicals (˙OH), superoxide anions (˙O) and HO and their derivatives with high efficiency, improve the viability of HO-treated chondrocytes by reducing their ROS levels, as well as lower their inflammatory factors. The above ability of antioxidation and inflammation resistance improved with the increase of carbazate substitution ratio. Significantly, this work provided the proof that modified Sephadex successfully alleviated the deterioration of cartilage and the progression of OA . The proposed microparticles showed a very promising capability for reducing ROS levels and further treating OA.
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http://dx.doi.org/10.1039/d1bm00743bDOI Listing
September 2021

ASTE1 promotes shieldin-complex-mediated DNA repair by attenuating end resection.

Nat Cell Biol 2021 08 5;23(8):894-904. Epub 2021 Aug 5.

Key Laboratory of Arrhythmias of the Ministry of Education of China, Research Center for Translational Medicine, East Hospital, Tongji University School of Medicine, Shanghai, China.

The shieldin complex functions as the downstream effector of 53BP1-RIF1 to promote DNA double-strand break end-joining by restricting end resection. The SHLD2 subunit binds to single-stranded DNA ends and blocks end resection through OB-fold domains. Besides blocking end resection, it is unclear how the shieldin complex processes SHLD2-bound single-stranded DNA and promotes non-homologous end-joining. Here, we identify a downstream effector of the shieldin complex, ASTE1, as a structure-specific DNA endonuclease that specifically cleaves single-stranded DNA and 3' overhang DNA. ASTE1 localizes to DNA damage sites in a shieldin-dependent manner. Loss of ASTE1 impairs non-homologous end-joining, leads to hyper-resection and causes defective immunoglobulin class switch recombination. ASTE1 deficiency also causes resistance to poly(ADP-ribose) polymerase inhibitors in BRCA1-deficient cells owing to restoration of homologous recombination. These findings suggest that ASTE1-mediated 3' single-stranded DNA end cleavage contributes to the control of DSB repair choice by 53BP1, RIF1 and shieldin.
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http://dx.doi.org/10.1038/s41556-021-00723-9DOI Listing
August 2021

Combined exposure to environmentally relevant copper and 2,2'-dithiobis-pyridine induces significant reproductive toxicity in male guppy (Poecilia reticulata).

Sci Total Environ 2021 Jul 20;797:149131. Epub 2021 Jul 20.

Colleges of Marine Life Sciences, Ocean University of China, Qingdao 266003, Shandong Province, China. Electronic address:

Metal pyrithiones (MePTs), the most widely used biocides in antifouling paints (AFs) coated on the hulls, are usually used in combination with Cu-containing substances. In the aquatic environment, 2,2'-dithiobis-pyridine ((PS)), the main degradation product of MePTs, and Cu usually coexist. However, their combined impacts on aquatic organisms are unclear. This study exposed male guppy (Poecilia reticulata) to an environmentally realistic concentration of Cu (10 μg/L) alone or Cu (10 μg/L) combined with 20, 200, and 2000 ng/L (PS) to explore their combined reproductive toxicity. The results showed that co-exposure to Cu and (PS) increased Cu accumulation in the fish body in a dose-dependent manner and induced obvious spermatozoon apoptosis and necrosis, which was mediated by the peroxidation and caspase activation. Compared to Cu alone, co-exposure to Cu and 200, 2000 ng/L (PS) significantly decreased the testosterone level and collapsed spermatogenesis, and depressed male's sexual interest and mating behavior were observed in three co-exposure groups. Moreover, co-exposure to Cu and (PS) increased the disturbance on cyp19a and cyp19b transcription and suppressed the "display" reproductive behavior. Eventually, co-exposure to Cu and (PS) caused male reproductive failure. Therefore, the concurrence of Cu and (PS) induced significant reproductive toxicity in male guppies and would threaten the sustainability of fish populations. Considering the extensive usage of MePTs products in the AFs, their ecological risk warrants more evaluation.
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http://dx.doi.org/10.1016/j.scitotenv.2021.149131DOI Listing
July 2021

Impact of the Stress Status of Employees on the Enterprise Technology Management Cost Through Matter-Element Analysis Under Psychological Health Education.

Front Psychol 2021 16;12:593813. Epub 2021 Jul 16.

School of Law, Chongqing University, Chongqing, China.

In this study, in order to analyze the stress sources and stress-coping strategies of employees in construction enterprises, to explore the influencing factors of enterprise technical management cost, and to offer suggestions for mental health education of employees, 372 employees of Shandong Construction Engineering Group Co., Ltd. were selected for a questionnaire survey. The influences of stress sources and stress-coping strategies on the mental health of employees were compared, based on different demographic variables. The evaluation model was constructed using the matter-element analysis to rank the factors influencing the enterprise technology management cost. The results showed that the stress value of work characteristics was the highest (4.26 ± 0.511), followed by the organizational structure and atmosphere (4.15 ± 0.382); stress-coping strategies at the individual level (1.84 ± 0.315) scored higher than that at the organizational level (1.67 ± 0.248) ( < 0.05). Notable differences were observed in balance between work and family between males and females ( < 0.05); in work characteristics, role orientation, personal relationship, and balance between work and family between subjects of different ages ( < 0.05); in work characteristics, and balance between work and family between the married and the unmarried ( < 0.05); and in role stress and work characteristics between subjects in different positions ( < 0.05). The evaluation results revealed that the factors influencing the technology management cost of enterprises included price index, development cost, fixed investment proportion, power equipment rate, mechanical artificial intelligence, labor cost, rate of technical equipment, the output value, informatization of technology management, and national policy. In conclusion, the two major sources of stress for employees in Luoyang Construction Engineering Group Co., Ltd. were as follows: (1) work characteristics and (2) organizational structure and atmosphere. Besides, many employees adopted the stress-coping strategies at the individual level, and enterprises needed to strengthen the psychological health education for employees at the organizational level. In practice, the enterprise needed to add importance to the development of mechanical artificial intelligence, informatization of technology management, and national policy.
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http://dx.doi.org/10.3389/fpsyg.2021.593813DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8322736PMC
July 2021

A Randomized, Phase III Study of Lenvatinib in Chinese Patients with Radioiodine-Refractory Differentiated Thyroid Cancer.

Clin Cancer Res 2021 Jul 29. Epub 2021 Jul 29.

Department of Thyroid and Neck Tumor, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin, China.

Purpose: Lenvatinib has shown efficacy in treating radioiodine-refractory differentiated thyroid cancer (RR-DTC) in the multinational phase III SELECT study; however, it has not been tested in Chinese patients with RR-DTC.

Experimental Design: Chinese patients with confirmed RR-DTC ( = 151) were randomly assigned 2:1 to receive lenvatinib 24 mg/day or placebo in 28-day cycles. The primary endpoint was progression-free survival, and key secondary endpoints included objective response rate and safety. Analyses for progression-free survival and objective response rate were conducted using Response Evaluation Criteria in Solid Tumors v1.1 and confirmed by independent imaging review. All adverse events were assessed and monitored.

Results: Progression-free survival was significantly longer with lenvatinib treatment [ = 103; median 23.9 months; 95% confidence interval (CI), 12.9-not estimable] versus placebo ( = 48; median 3.7 months; 95% CI, 1.9-5.6; hazard ratio = 0.16; 95% CI, 0.10-0.26; < 0.0001). The objective response rate was 69.9% (95% CI, 61.0-78.8) in the lenvatinib arm and 0% (95% CI, 0-0) in the placebo arm. At data cutoff, 60.2% of patients receiving lenvatinib remained on treatment; treatment-emergent adverse events led to lenvatinib discontinuation in 8.7% of patients. Overall, treatment-emergent adverse events of grade ≥3 occurred in 87.4% of patients in the lenvatinib arm, the most common being hypertension (62.1%) and proteinuria (23.3%).

Conclusions: Lenvatinib at a starting dose of 24 mg/day significantly improved progression-free survival and objective response rate in Chinese patients with RR-DTC versus placebo. There were no new or unexpected toxicities. Results are consistent with those from SELECT involving patients with RR-DTC.
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http://dx.doi.org/10.1158/1078-0432.CCR-21-0761DOI Listing
July 2021

Sequential Catalytic Functionalization of Aryltriazenyl Aldehydes for the Synthesis of Complex Benzenes.

ACS Catal 2021 May 5;11(10):6091-6098. Epub 2021 May 5.

Department of Chemistry, Chemistry Research Laboratory, University of Oxford, Mansfield Road, Oxford, OX1 3TA, United Kingdom.

We demonstrate that aryltriazenes can promote three distinctive types of C-H functionalization reactions, allowing the preparation of complex benzene molecules with diverse substitution patterns. 2-Triazenylbenzaldehydes are shown to be efficient substrates for Rh(I)-catalyzed intermolecular alkyne hydroacylation reactions. The resulting triazene-substituted ketone products can then undergo either a Rh(III)-catalyzed C-H activation, or an electrophilic aromatic substitution reaction, achieving multifunctionalization of the benzene core. Subsequent triazene derivatization provides traceless products.
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http://dx.doi.org/10.1021/acscatal.1c01722DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8291607PMC
May 2021

AGTH-Net: Attention-Based Graph Convolution-Guided Third-Order Hourglass Network for Sports Video Classification.

J Healthc Eng 2021 6;2021:8517161. Epub 2021 Jul 6.

College of Sports Engineering and Information Technology, Wuhan Sports University, Wuhan 430079, China.

As a hot research topic, sports video classification research has a wide range of applications in switched TV, video on demand, smart TV, and other fields and is closely related to people's lives. Under this background, sports video classification research has aroused great interest in people. However, the existing methods usually use manual video classification, which the workers themselves often influence. It is challenging to ensure the accuracy of the results, leading to the wrong classification. Due to these limitations, we introduce neural network technology to the automatic classification of sports. This paper proposed a novel attention-based graph convolution-guided third-order hourglass network (AGTH-Net) classification model. First, we designed a kind of figure convolution model based on the attention mechanism. The model is the key to introduce the attention mechanism for neighborhood node weights' allocation. It reduces the impact of error nodes in the neighborhood while avoiding manual weight assignment. Second, according to the sports complex video image characteristics, we use the third-order hourglass network structure. It is used for the extraction and fusion of multiscale characteristics of sports. In addition, in the hourglass, internal network residual-intensive modules are introduced, realizing characteristics in different levels of network transfer and reuse. It is helpful for maximum details to feature extracting and enhancing the network expression ability. Comparison and ablation experiments are also carried out to prove the effectiveness and superiority of the proposed algorithm.
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http://dx.doi.org/10.1155/2021/8517161DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8279861PMC
July 2021

Nonlinear Dynamic Analysis of Axially Moving Laminated Shape Memory Alloy Beam with 1:3 Internal Resonance.

Materials (Basel) 2021 Jul 18;14(14). Epub 2021 Jul 18.

Laboratory of Test and Testing Technology, Tianjin Key Laboratory of Microgravity and Hypogravity Environment Simulation Technology, Tianjin 300384, China.

The remarkable properties of shape memory alloys (SMA) are attracting significant technological interest in many fields of science and engineering. In this paper, a nonlinear dynamic analytical model is developed for a laminated beam with a shape memory alloy layer. The model is derived based on Falk's polynomial model for SMAs combined with Timoshenko beam theory. In addition, axial velocity, axial pressure, temperature, and complex boundary conditions are also parameters that have been taken into account in the creation of the SMA dynamical equation. The nonlinear vibration characteristics of SMA laminated beams under 1:3 internal resonance are studied. The multi-scale method is used to solve the discretized modal equation system, the characteristic equation of vibration modes coupled to each other in the case of internal resonance, as well as the time-history and phase diagrams of the common resonance amplitude in the system are obtained. The effects of axial velocity and initial conditions on the nonlinear internal resonance characteristics of the system were also studied.
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http://dx.doi.org/10.3390/ma14144022DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8304594PMC
July 2021

Upregulation of MicroRNA-34a Sensitizes Ovarian Cancer Cells to Resveratrol by Targeting Bcl-2.

Yonsei Med J 2021 Aug;62(8):691-701

Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Anhui Medical University, Hefei, China.

Purpose: Resveratrol (REV), a natural compound found in red wine, exhibits antitumor activity in various cancers, including ovarian cancer (OC). However, its potential anti-tumor mechanisms in OC are not well characterized. Here, we tried to elucidate the underlying mechanisms of REV in OC cells.

Materials And Methods: The anti-proliferative effects of REV against OC cells were measured using CCK-8 assay. Apoptosis was measured using an Annexin V-FITC/PI apoptosis detection kit. The anti-metastasis effects of REV were evaluated by invasion assay and wound healing assay. The miRNA profiles in REV-treated cells were determined by microarray assay.

Results: Our results showed that REV treatment suppresses the proliferation, induces the apoptosis, and inhibits the invasion and migration of OV-90 and SKOV-3 cells. miR-34a was selected for further study due to its tumor suppressive roles in various human cancers. We found miR-34a overexpression enhanced the inhibitory effects of REV on OC cells, whereas miR-34a inhibition had the opposite effect in OC cells. In addition, we verified that , an anti-apoptotic gene, was found directly targeted by miR-34a. We also found that REV reduced the expression of Bcl-2 in OC cells. Further investigations revealed that overexpression of Bcl-2 significantly abolished the anti-tumor effects of REV on OC cells.

Conclusion: Overall, these results demonstrated that REV exerts anti-cancer effects on OC cells through an miR-34a/Bcl-2 axis, highlighting the therapeutic potential of REV for treatment of OC.
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http://dx.doi.org/10.3349/ymj.2021.62.8.691DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8298871PMC
August 2021

Progress in research and development of particle electrodes for three-dimensional electrochemical treatment of wastewater: a review.

Environ Sci Pollut Res Int 2021 Sep 22;28(35):47800-47824. Epub 2021 Jul 22.

Department of Environmental Science and Engineering, School of Energy and Environmental Engineering, University of Science and Technology Beijing, 30 Xueyuan Road, Haidian District, Beijing, 100083, China.

A three-dimensional (3D) electrochemical technology is regarded as a very effective industrial wastewater treatment method as it has high treatment efficiency, high current efficiency, and low energy consumption, and especially can completely mineralize nonbiodegradable organic pollutants. The core of the 3D electrochemical technology is a particle electrode, and the particle electrode plays several important roles for removing pollutants during the electrochemical reaction process. Many types of particle electrodes have been developed and used for different types of wastewater treatment. In this paper, a comprehensive review on the research and development of particle electrodes of the 3D electrochemical reactors for wastewater treatment is conducted. Specifically, the role that the particle electrode plays during the 3D electrochemical treatment of wastewater is thoroughly investigated and systematized. In addition, the different types of particle electrodes used in the 3D electrochemical wastewater treatment are classified into several types according to the presence or absence of a catalyst and the main components of the particle electrode or carrier. Also, focusing on the recent research results, the structural characteristics, performance, advantages and defects, and the role of catalyst components of each particle electrodes are evaluated. Finally, the direction and prospect of future research on the particle electrode is presented.
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http://dx.doi.org/10.1007/s11356-021-13785-xDOI Listing
September 2021

Magnetic Resonance Imaging-Based Structural Covariance Changes of the Striatum in Lifelong Premature Ejaculation Patients.

J Magn Reson Imaging 2021 Jul 22. Epub 2021 Jul 22.

Life Sciences Research Center, School of Life Science and Technology, Xidian University, Xi'an, China.

Background: The striatum has been reported to be implicated in various neurological diseases, including lifelong premature ejaculation (LPE). Altered striatum-related functional connectivity was investigated in LPE patients in previous studies; however, structural abnormalities in the striatum have been less studied in LPE.

Purpose: To identify the gray matter volume (GMV) and structural covariance patterns of the striatum between LPE patients and healthy controls (HCs).

Study Type: Prospective.

Subjects: Forty-three LPE patients and 31 male HCs.

Field Strength/sequence: 3.0 T magnetic resonance imaging (MRI) scanner; T1-weighted imaging using a spoiled gradient recalled echo sequence.

Assessment: Preprocessing of structural MRI data and the striatum-seeded GMV computation were conducted using SPM12.

Statistical Tests: Two sample t-test was used to compare differences in GMV of the striatum between patients and HCs. Regions showing altered between-group GMV were considered as seeds for structural covariance analysis in two groups. Additionally, correlations between GMV findings and clinical features were assessed with age and total intracranial volume (TIV) as covariates and with age, TIV, anxiety, and depression scores as covariates in the patient group, P < 0.05 was considered statistically significant.

Results: Compared to HCs, LPE patients had significantly decreased GMV in four regions located in the bilateral caudate and putamen. Distinct striatum-based structural covariance patterns in the two groups were mainly related to the thalamus, amygdala, insula, anterior cingulate cortex, middle cingulate cortex, medial prefrontal cortex, primary motor cortex, and precuneus/cuneus. LPE patients showed that GMV in the bilateral caudate negatively correlated with the premature ejaculation diagnostic tool (PEDT) scores (r = -0.369, r = -0.377, respectively).

Data Conclusion: Our findings indicated that LPE patients had altered GMV and structural covariance patterns in the striatum compared to HCs. The correlations between abnormal GMV and PEDT were also shown in the present findings. These findings may contribute to enhancing the understanding of the pathophysiology of LPE.

Level Of Evidence: 1 TECHNICAL EFFICACY: Stage 3.
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http://dx.doi.org/10.1002/jmri.27851DOI Listing
July 2021

Levo-tetrahydropalmatine inhibits α4β2 nicotinic receptor response to nicotine in cultured SH-EP1 cells.

Acta Pharmacol Sin 2021 Jul 12. Epub 2021 Jul 12.

Department of Neurobiology, Barrow Neurological Institute, St. Joseph's Hospital and Medical Center, Phoenix, AZ, 85013, USA.

Nicotine, a major component of tobacco, is highly addictive and acts on nicotinic acetylcholine receptors (nAChRs) to stimulate reward-associated circuits in the brain. It is well known that nAChRs play critical roles in mediating nicotine reward and addiction. Current FDA-approved medications for smoking cessation are the antidepressant bupropion and the nicotinic partial agonist varenicline, yet both are limited by adverse side effects and moderate efficacy. Thus, development of more efficacious medications with fewer side effects for nicotine addiction and smoking cessation is urgently needed. l-Tetrahydropalmatine (l-THP) is an active ingredient of the Chinese medicinal herb Corydalis ambigua that possesses rich neuropharmacological actions on dopamine (DA) receptors in the mesocorticolimbic dopaminergic reward pathway. L-THP has been explored as anti-addiction treatments for drug abuse including nicotine. However, the targets and mechanisms of l-THP-caused anti-nicotine effects are largely unknown. In this study we address this question by elucidating the effects of l-THP on human neuronal nAChRs using patch-clamp recordings. Human neuronal α4β2-nAChRs were heterologously expressed in SH-EP1 human epithelial cells. Bath application of nicotine (0.1-100 μM) induced inward currents, co-application of l-THP (3 μM) inhibited nicotine-induced currents in the transfected cells. L-THP-caused inhibition was concentration-dependent (the EC values for inhibiting the peak and steady-state current were 18 and 2.1 μM, respectively) and non-competitive. Kinetic analysis of the whole-cell currents showed that l-THP slowed rising time and accelerated decay time constants. L-THP specifically modulated α4β2-nAChRs, as it did not affect α7-nAChRs or α1*-nAChRs (muscle type). Interestingly, two putative α4β2-nAChR isoforms, namely sazetidine A-activated, high-sensitive one (α4β2-nAChR) and cytisine-activated, low-sensitive one (α4β2-nAChR) were pharmacologically separated, and the low-sensitive one was more susceptible to l-THP inhibition than the high-sensitive one. In conclusion, we demonstrate that l-THP blocks neuronal α4β2-nAChR function, which may underlie its inhibition on nicotine addiction.
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http://dx.doi.org/10.1038/s41401-021-00709-1DOI Listing
July 2021

Higher inter-hemispheric homotopic connectivity in lifelong premature ejaculation patients: a pilot resting-state fMRI study.

Quant Imaging Med Surg 2021 Jul;11(7):3234-3243

Life Science Research Center, School of Life Science and Technology, Xidian University, Xi'an, China.

Background: Lifelong premature ejaculation (PE) is one common male sexual dysfunction and is implicated in widespread structural and functional abnormalities of bilateral hemispheres. However, whether the inter-hemisphere functional connectivity (FC) of lifelong PE patients was altered still remain unclear.

Methods: Thirty-four lifelong PE patients and 30 healthy controls (HCs) were enrolled in this study and all underwent T1-weighted and resting-state functional MRI (fMRI) scan. The voxel-mirrored homotopic connectivity (VMHC) measure and independent sample -test were applied to examine the alterations of VMHC values in the patients relative to HCs with the significant threshold at P<0.05, false discovery rates corrected. Correlation analysis was adopted to calculate the relationships between the imaging results and clinical characteristics of patients (P<0.05, Bonferroni corrected). Receiver operating characteristic (ROC) curve analysis was performed to investigate the possible biomarkers for distinguishing the patients from the HCs using the VMHC values of inter-group differences.

Results: The results revealed that compared with HCs, lifelong PE patients had higher VMHC values in the precentral gyrus (PG), primary somatosensory cortex (S1), supplementary motor area (SMA), precuneus, middle temporal cortex (MTC), superior temporal pole (STP), thalamus, caudate and middle cingulate cortex (MCC). Correlation analysis showed that the mean VMHC values in the S1 negatively correlated with intravaginal ejaculation latency time (IELT) in the patient group. Furthermore, the caudate revealed the well classification power from the ROC analysis.

Conclusions: The present study showed the abnormal inter-hemisphere interaction and integration of information involved in ejaculation inhibitory control, sensorimotor mediation and self-reference processing including the thalamus, caudate, MCC, widespread parietal cortex and temporal cortex in lifelong PE patients compared with HCs. Correlation analysis and ROC analysis revealed the importance of S1 and caudate in lifelong PE. Notably, the ROC result of caudate might show the core roles of caudate played in the pathophysiology of lifelong PE.
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http://dx.doi.org/10.21037/qims-20-1103DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8250033PMC
July 2021
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