Publications by authors named "Ming Chang"

266 Publications

Interactions between α-tocopherol and γ-oryzanol in oil-in-water emulsions.

Food Chem 2021 Mar 22;356:129648. Epub 2021 Mar 22.

National Engineering Research Center for Functional Food, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, PR China.

The interaction between antioxidants is affected by many factors, such as concentration, ratio and system. In this study, different concentrations of α-tocopherol and γ-oryzanol showed antagonistic effect in the oil-in-water emulsion, and the distribution of α-tocopherol increased in aqueous phase after combined with γ-oryzanol. The concentration could affect the degree of antagonism. According to fluorescence quenching, cyclic voltammetry measurements and the oxidative decomposition of antioxidants during storage, the inhibitory effect of γ-oryzanol on the regeneration of α-tocopherol was proposed to be responsible for the antagonism. This work can provide suggestions for studying the mechanism of antioxidant interaction in emulsion system.
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http://dx.doi.org/10.1016/j.foodchem.2021.129648DOI Listing
March 2021

[Characteristics and Meteorological Factors of Complex Nonattainment Pollution of Atmospheric Photochemical Oxidant (O) and PM in the Pearl River Delta Region, China].

Huan Jing Ke Xue 2021 Apr;42(4):1600-1614

Shenzhen Environmental Monitoring Center, Shenzhen 518049, China.

Based on the atmospheric pollutant data from twelve monitoring sites in the Guangdong-Hong Kong-Macao Pearl River Delta Regional Air Quality Monitoring Network, the mass concentration trends of atmospheric photochemical oxidants (O, NO+O) and PM during 2013-2017 were studied. The complex nonattainment pollution of O and PM is defined as the daily average mass concentration of NO and PM and daily maximum 8 h average (O MDA8) mass concentration of O simultaneously that exceeds the Chinese grade Ⅱ national air quality standard. The characteristics and meteorological factors that influence the complex nonattainment pollution of O and PM at different types of areas were analyzed. The results indicate that from 2013 to 2017, the annual average mass concentration of PM in the Pearl River Delta (PRD) region decreased from (44±7) μg·m to (32±4) μg·m, which met the annual standard for three consecutive years. The annual average mass concentration of O decreased from (127±14) μg·m in 2013 to (114±12) μg·m in 2016 and then showed a general rebound trend to (129±13) μg·m in 2017 when O concentrations increased significantly (10 μg·m). The proportion of pollution processes with O as the primary pollutant increased from 33% in 2013 to 78% in 2017, and the regional characteristics of simultaneous pollution in multiple cities have been highlighted. The complex nonattainment pollution of O and PM occurred 60 times during the study period, primarily in urban sites (78%) and suburban sites (22%). The largest number of days of complex nonattainment pollution occurred in autumn (52%) because of strong solar radiation that was conducive to ozone formation, and consequently, the high oxidization of the atmosphere promoted the secondary generation of PM. The weather conditions that caused the complex nonattainment pollution in the PRD mainly include outflow-high-pressures (43%), subtropical-high-pressures(30%), and tropical-depressions (27%). In terms of specific meteorological conditions, when the temperature was in the range of 20-25℃ and relative humidity was in the range of 60%-75%, the proportion of complex nonattainment pollution was the highest (22%). When O pollution was substantial, the high relative humidity and low wind speed during the nighttime caused the concentration of NO and PM to rise significantly, and then the high temperatures during the day aggravated the complex nonattainment pollution.
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http://dx.doi.org/10.13227/j.hjkx.202007286DOI Listing
April 2021

Perdisulfate-assisted advanced oxidation of 2,4-dichlorophenol by bio-inspired iron encapsulated biochar catalyst.

J Colloid Interface Sci 2021 Jun 20;592:358-370. Epub 2021 Feb 20.

State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, PR China.

To improve advanced oxidation processes (AOPs), bio-inspired iron-encapsulated biochar (bio-inspired Fe⨀BC) catalysts with superior performance were prepared from iron-rich biomass of Iris sibirica L. using a pyrolysis method under anaerobic condition. The obtained compounds were used as catalysts to activate perdisulfate (PDS) and then degradate 2,4-dichlorophenol (2,4-DCP), and synthetic iron-laden biochar (synthetic Fe-BC) was used for comparison. The highest removal rate of 2,4-DCP was 98.35%, with 37.03% of this being distinguished as the contribution of micro-electrolysis, greater than the contribution of adsorption (32.81%) or advanced oxidation (28.51%). The high performance of micro-electrolysis could be attributable to the formation of Fe (Iron, syn) and austenite (CFe) with strong electron carrier at 700 °C. During micro-electrolysis, Fe and electrons were gradually released and then used as essential active components to enhance the AOPs. The slow-releasing Fe (K = 0.0048) also inhibited the overconsumption of PDS (K = -0.00056). Furthermore, the electrons donated from Fe⨀BC-4 were able to activate PDS directly. The electrons were enriched by the porous structure of Fe⨀BC-4, and the formation of the COFe bond in the π-electron system could also accelerate the electron transfer to activate PDS. Similar reactive oxygen species (ROS) were identified during the micro-electrolysis and AOPs, leading to similar degradation pathways. The higher does concentration of O generated during micro-electrolysis than during the AOPs also led to a greater dechlorination effect.
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http://dx.doi.org/10.1016/j.jcis.2021.02.056DOI Listing
June 2021

Danggui Buxue Decoction enhances the anticancer activity of gemcitabine and alleviates gemcitabine-induced myelosuppression.

J Ethnopharmacol 2021 Jun 25;273:113965. Epub 2021 Feb 25.

Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing, 210009, China. Electronic address:

Ethnopharmacological Relevance: Danggui Buxue Decoction (DBD) as a traditional Chinese medicine (TCM) has been widely used to treat blood deficiency. With the immune regulation and hematopoietic effect, DBD improved the quality of life in non-small-cell lung cancer (NSCLC) patients. We previously reported that DBD sensitized the response of NSCLC to Gemcitabine (Gem); however, the synergism and attenuation mechanism on the combination of Gem and DBD has not yet been elucidated.

Aim Of The Study: To investigate the mechanisms of DBD in enhancing the anticancer activity of Gem and alleviating Gem-induced myelosuppression.

Materials And Methods: A549 nude mice model was established to study the effect on the combination of Gem and DBD. The organ indices, peripheral blood cells and the hematopoiesis-related cytokines were analyzed in Gem-induced myelosuppressive mice. Then we studied the whole process from Gem-induced bone marrow suppression to self-healing, and the mechanism of DBD's attenuation by the experiments of bone marrow nucleated cells (BMNCs).

Results: There were an enhanced anticancer effect and an improvement of hematopoietic function by combining of Gem and DBD in A549 nude mice model. DBD regulated Hu antigen R (HuR), deoxycytidine kinase (dCK) and nuclear factor erythroid 2-related factor (Nrf2), increased the expression of thrombopoietin (TPO) and granulocyte-macrophage colony stimulating factor (GM-CSF). For Gem-induced myelosuppressive mice, DBD improved the number of peripheral blood cells and the levels of hematopoiesis-related cytokines. Moreover, DBD was observed to reduce deoxyribonucleic acid (DNA) content at the G1 phase, promoted BMNCs proliferation and up-regulated cycle-related proteins.

Conclusions: The results indicated that DBD not only improved the sensitivity of Gem but also alleviated Gem-induced myelosuppression. This study may provide a pharmacological basis for the combination of DBD and Gem in clinical application.
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http://dx.doi.org/10.1016/j.jep.2021.113965DOI Listing
June 2021

Release Mechanism, Secondary Pollutants and Denitrification Performance Comparison of Six Kinds of Agricultural Wastes as Solid Carbon Sources for Nitrate Removal.

Int J Environ Res Public Health 2021 01 29;18(3). Epub 2021 Jan 29.

Research Center of Environmental Pollution Control Technology, Chinese Research Academy of Environmental Science, Beijing 100012, China.

Agricultural wastes used as denitrification carbon sources have some drawbacks such as excessive organic carbon release and unclear release characteristics of nitrogen, phosphorus, and chromatic substances, which can cause adverse effects on the effluent quality during the denitrification process. The composition and surface characteristics, carbon release mechanisms, and secondary pollutant release properties of six kinds of agricultural wastes, i.e., rice straw (RS), wheat straw (WS), corn stalk (CS), corncob (CC), soybean stalk (SS), and soybean hull (SH) were studied and analyzed in this research. The denitrification performance of these agricultural wastes was also investigated extensively by batch experiments. The results showed that the carbon release basically followed the second-order reaction kinetic equation and Ritger-Peppas equation in the 120 h reaction, and it was mainly controlled by the diffusion process. The kinetic equation fitting results and bioavailability test suggested that the potential risk of excessive effluent COD of CC was the lowest due to the appropriate amount and degradability of its released carbon. The NH-N, TN, and TP in the leachate of RS were higher than those of the other five agriculture wastes, and the chroma in the leachate of WS and CS was heavier than that of the others. CC released the lowest pollutants, which resulted in slight fluctuations of effluent quality in the start-up period (1-11 d), and it had the best nitrogen removal capacity in the denitrification experiment. The average NO-N removal of CC was 5.12 mg for each batch in the stable period (11-27 d), which was higher than that of others, and less NO-N, NH-N, and COD were accumulated in the CC effluent during the whole denitrification process.
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http://dx.doi.org/10.3390/ijerph18031232DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7908289PMC
January 2021

The negative regulation of retinoic acid-inducible gene I (RIG-I)-like receptors (RLRs) signaling pathway in fish.

Authors:
Ming Xian Chang

Dev Comp Immunol 2021 Jun 4;119:104038. Epub 2021 Feb 4.

State Key Laboratory of Freshwater Ecology and Biotechnology, Key Laboratory of Aquaculture Disease Control, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei Province, 430072, China; Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, China; University of Chinese Academy of Sciences, Beijing, China. Electronic address:

At each stage of innate immune response, there are stimulatory and inhibitory signals that modulate the strength and character of the response. RIG-I-like receptor (RLR) signaling pathway plays pivotal roles in antiviral innate immune response. Recent studies have revealed the molecular mechanisms that viral infection leads to the activation of RLRs-mediated downstream signaling cascades and the production of type I interferons (IFNs). However, antiviral immune responses must be tightly regulated in order to prevent detrimental type I IFNs production. Previous reviews have highlighted negative regulation of RLR signaling pathway, which mainly target to directly regulate RIG-I, MDA5, MAVS and TBK1 function in mammals. In this review, we summarize recent advances in our understanding of negative regulators of RLR signaling pathway in teleost, with specific focus on piscine and viral regulatory mechanisms that directly or indirectly inhibit the function of RIG-I, MDA5, LGP2, MAVS, TRAF3, TBK1, IRF3 and IRF7 both in the steady state or upon viral infection. We also further discuss important directions for future studies, especially for non-coding RNAs and post-translational modifications via fish specific TRIM proteins. The knowledge of negative regulators of RLR signaling pathway in teleost will shed new light on the critical information for potential therapeutic purposes.
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http://dx.doi.org/10.1016/j.dci.2021.104038DOI Listing
June 2021

New perspective toward nutritional support for malnourished cancer patients: Role of lipids.

Compr Rev Food Sci Food Saf 2021 Mar 2;20(2):1381-1421. Epub 2021 Feb 2.

National Engineering Research Center for Functional Food, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, China.

To improve the difficulties related to malnutrition, nutritional support has become an essential part of multidisciplinary comprehensive treatment for cancer. Lipids are essential nutrient source for the human body, and nowadays in clinical practices, it has a positive interventional effect on patients suffering from cancer. However, contribution of lipids in nutritional support of cancer patients is still poorly understood. Moreover, the sensory and physicochemical properties of lipids can severely restrict their applications in lipid-rich formula foods. In this review article, for the first time, we have presented a summary of the existing studies which were related to the associations between different lipids and improved malnutrition in cancer patients and discussed possible mechanisms. Subsequently, we discussed the challenges and effective solutions during processing of lipids into formula foods. Further, by considering existing problems in current lipid nutritional support, we proposed a novel method for the treatment of malnutrition, including developing individualized lipid nutrition for different patients depending on the individual's genotype and enterotype. Nonetheless, this review study provides a new direction for future research on nutritional support and the development of lipid-rich formula foods for cancer patients, and probably will help to improve the efficacy of lipids in the treatment of cancer malnutrition.
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http://dx.doi.org/10.1111/1541-4337.12706DOI Listing
March 2021

Long-term Experience and Outcomes of Programmatic Antiretroviral Therapy for Human Immunodeficiency Virus Type 2 Infection in Senegal, West Africa.

Clin Infect Dis 2021 Feb;72(3):369-378

Division of Allergy and Infectious Diseases, Department of Medicine, University of Washington, Seattle, Washington, USA.

Background: Programmatic treatment outcome data for people living with human immunodeficiency virus type 2 (HIV-2) in West Africa, where the virus is most prevalent, are scarce.

Methods: Adults with HIV-2 initiating or receiving antiretroviral therapy (ART) through the Senegalese national AIDS program were invited to participate in this prospective, longitudinal observational cohort study. We analyzed HIV-2 viral loads, CD4 cell counts, antiretroviral drug resistance, loss to follow-up, and mortality. We also examined changes in treatment guidelines over time and assessed progress toward the Joint United Nations Programme on HIV/AIDS (UNAIDS) 90-90-90 targets for HIV-2.

Results: We enrolled 291 participants at 2 sites for 926.0 person-years of follow-up over 13 years. Median follow-up time was 2.2 years per participant. There were 21 deaths reported (7.2%), and 117 individuals (40.2%) were lost to follow-up, including 43 (14.7%) who had an initial visit but never returned for follow-up. CD4 counts and HIV-2 viral suppression (< 50 copies/mL) at enrollment increased over calendar time. Over the study period, 76.7% of plasma viral loads for participants receiving ART were suppressed, and median CD4 gain was 84 cells/μL in participants' first 2 years on study. Since the UNAIDS 90-90-90 strategy was published, 88.1% of viral loads were suppressed. Fifteen percent of patients experienced virologic failure with no known resistance mutations, while 56% had evidence of multiclass drug resistance.

Conclusions: Participants in the Senegalese national AIDS program are initiating ART earlier in the course of disease, and more modern therapeutic regimens have improved outcomes among those receiving therapy. Despite these achievements, HIV-2 treatment remains suboptimal, and significant challenges to improving care remain.
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http://dx.doi.org/10.1093/cid/ciaa277DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7850514PMC
February 2021

Enzymatic enrichment of n-3 polyunsaturated fatty acid glycerides by selective hydrolysis.

Food Chem 2021 Jun 1;346:128743. Epub 2020 Dec 1.

Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, National Engineering Research Center for Functional Food, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road Wuxi, Jiangsu 214122, PR China. Electronic address:

Most natural oils are low in n-3 polyunsaturated fatty acids (n-3 PUFAs) content, which limits their application in health products. In this study, n-3 PUFAs in glyceride form were selectively enriched by lipase-mediated hydrolysis of n-3 PUFA-containing oils. First, commercial lipases were screened, and the lipase AY "Amano" 400SD from Candida cylindracea was the best choice in producing n-3 PUFA glycerides from tuna oil. Subsequently, the hydrolysis conditions were optimized. Under the optimal conditions, the highest n-3 PUFA content in the glyceride fraction was found to be 57.7% after enzymatic hydrolysis. Addition of Ca to the system significantly shortened the reaction time from 10 to 4 h. When algal oil was used as substrate, total PUFA contents in the glyceride fraction were 89.9%. This study provides an efficient enzymatic process to produce n-3 PUFA-enriched glyceride concentrates and demonstrates that AY "Amano" 400SD can effectively discriminate against n-3 PUFAs during hydrolysis.
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http://dx.doi.org/10.1016/j.foodchem.2020.128743DOI Listing
June 2021

The spatial distribution characteristics of coseismic landslides triggered by the Ms7.0 Lushan earthquake and Ms7.0 Jiuzhaigou earthquake in southwest China.

Environ Sci Pollut Res Int 2021 Jan 6. Epub 2021 Jan 6.

State Key Laboratory of GeoHazard Prevention and GeoEnvironment Protection, Chengdu University of Technology, Chengdu, 610059, China.

In recent years, a series of earthquakes have occurred in Sichuan province, southwest China. On April 20, 2013, 1013 landslides were triggered in the Lushan earthquake within the research area of 1215.87 km. On August 8, 2017, 821 landslides were induced in the Jiuzhaigou earthquake within the study area of 541.61 km. The slope, aspect, elevation, distance to the river, distance to fault, stratum lithology, curvature, and PGA as evaluation factors were chosen to research the distribution characteristics of coseismic landslides. The relationship between influence factors and distribution of coseismic landslides were analyzed using landslide area density (LAD), defined as the ratio between the coseismic landslides area and the total area under the specific classification of eight factors, and landslide number density (LND), interpreted as the number of landslides per square kilometer affected by an earthquake. Both the LAD and LND curves show the common correlations between distribution characteristics of coseismic landslides and the classification of eight factors. The high density of coseismic landslides was found at locations where the valley evolves from U-shaped to V-shaped, along slopes with an inclination between 20 and 50°. Due to the effect of river cutting erosion and human engineering activities, massive coseismic landslides appeared within the scope of 200 m from the river system. Eight hundred sixty small shallow coseismic landslides were found less than 3 km from the seismogenic fault in both earthquakes. Both concave and convex terrain has the same effect of promoting coseismic landslides. The strata of the Carboniferous and Cretaceous are more prone to coseismic landslides occurrence. In the event of another earthquake of similar magnitude in southwest China in the future, the research results can quickly predict the distribution of coseismic landslides and provide a scientific basis for emergency rescue.
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http://dx.doi.org/10.1007/s11356-020-11826-5DOI Listing
January 2021

Dietary oleic acid supplementation and blood inflammatory markers: a systematic review and meta-analysis of randomized controlled trials.

Crit Rev Food Sci Nutr 2020 Dec 11:1-18. Epub 2020 Dec 11.

Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, School of Food Science and Technology, Jiangnan University, Wuxi, China.

The aim of this systematic review and meta-analysis was to analyze data from randomized controlled trials (RCTs) assessing the effects of oleic acid (OA) supplementation on blood inflammatory markers in adults. PubMed, EMBASE and Cochrane Library databases were systematically searched from 1950 to 2019, with adults and a minimum intervention duration of 4 weeks. The effect size was estimated, adopting standardized mean difference (SMD) and 95% confidence interval (CI). Of the 719 identified studies, thirty-one RCTs involving 1634 subjects were eligible. The results of this study revealed that increasing OA supplementation significantly reduced C-reactive protein (CRP) (SMD: -0.11, 95% CI: -0.21, -0.01, P = 0.038). However, dietary OA consumption did not significantly affect tumor necrosis factor (TNF) (SMD: -0.05, 95% CI: -0.19, 0.10, P = 0.534), interleukin 6 (IL-6) (SMD: 0.01, 95% CI: -0.10, 0.13, P = 0.849), fibrinogen (SMD: 0.08, 95% CI: -0.16, 0.31, P = 0.520), plasminogen activator inhibitor type 1 (PAI-1) activity (SMD: -0.11, 95% CI: -0.34, 0.12, P = 0.355), soluble intercellular adhesion molecule-1 (sICAM-1) (SMD: -0.06, 95% CI: -0.26, 0.13, P = 0.595) or soluble vascular cell adhesion molecule-1 (sVCAM-1) (SMD: -0.04, 95% CI: -0.27, 0.18, P = 0.701). Overall, the meta-analysis demonstrated that dietary OA supplementation significantly reduced CRP, yet did not affect other inflammatory markers including TNF, IL-6, fibrinogen, PAI-1 activity, sICAM-1or sVCAM-1.
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http://dx.doi.org/10.1080/10408398.2020.1854673DOI Listing
December 2020

Pneumonia in endangered aquatic mammals and the need for developing low-coverage vaccination for their management and conservation.

Anim Health Res Rev 2020 12 9;21(2):122-130. Epub 2020 Dec 9.

State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, The Chinese Academy of Sciences, Wuhan430072, Hubei Province, People's Republic of China.

Anthropogenic activities can lead to several devastating effects on the environment. The pollutants, which include the discharge of effluents, runoffs in the form of different lethal and sub-lethal concentrations of pesticides, heavy metals, and other contaminants, can harm exposed fauna and flora. The aquatic environment is the ultimate destination for many pollutants which negatively affect aquatic biodiversity and even can cause a species to become extinct. A pollutant can directly affect the behavior of an animal, disrupt cellular systems, and impair the immune system. This harm can be reduced and even mitigated by adopting proper approaches for the conservation of the target biota. Among aquatic organisms, cetaceans, such as the Yangtze finless porpoise, Irrawaddy dolphin, Ganges River dolphin, Amazon River dolphin, and Indus River dolphin, are at a higher risk of extinction because of lack of knowledge and research, and thus insufficient information with respect to their conservation status, management, and policies. Pneumonia is one of the leading causes of mass mortalities of cetaceans. This article reviews the limited research reported on stress and pneumonia induced by pollution, stress-induced pneumonia and immunosuppression, pneumonia-caused mass mortalities of aquatic mammals, and vaccination in wildlife with a specific focus on aquatic mammals, the role of genomics in vaccine development and vaccination, and the major challenges in vaccine development for biodiversity conservation.
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http://dx.doi.org/10.1017/S1466252320000158DOI Listing
December 2020

The function of zebrafish gpbar1 in antiviral response and lipid metabolism.

Dev Comp Immunol 2021 Mar 5;116:103955. Epub 2020 Dec 5.

State Key Laboratory of Freshwater Ecology and Biotechnology, Key Laboratory of Aquaculture Disease Control, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei Province, 430072, China; Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, China; University of Chinese Academy of Sciences, Beijing, China. Electronic address:

G protein-coupled bile acids receptor 1 (GPBAR1 or TGR5) has been widely studied as a metabolic regulator involved in bile acids synthesis, glucose metabolism and energy homeostasis. Several recent studies have shown that mammalian GPBAR1 is also involved in antiviral innate immune responses. However, the functions of piscine GPBAR1 in antibacterial or antiviral immune responses and lipid metabolism remain unclear. In the present study, we report the functional characterization of zebrafish gpbar1. Similar to mammalian GPBAR1, zebrafish gpbar1 contains similar domain composition, shows a dose-dependent activation by bile acids including INT777, LCA, DCA, CDCA and CA, and can be induced by viral infection. Compared with corresponding control groups, a significant antiviral activity against spring viremia of carp virus (SVCV) infection was observed in ZF4 cells overexpressing zebrafish gpbar1 with INT777 treatment, but not in ZF4 cells overexpressing zebrafish gpbar1 without INT777 treatment. The activation of zebrafish gpbar1 had no significant antibacterial effect against Edwardsiella piscicida infection in ZF4 cells in vitro. Transcriptome analysis revealed that zebrafish gpbar1 activation played a crucial role in activating RLR signaling pathway and inducing the production of ISGs, but not for bile acid biosynthesis and transportation. The co-occurrence analysis for antiviral-related and bile acids metabolism-related DEGs suggested a strong interaction among 2 bile acid receptors (gpbar1 and nr1h4), slco2b1 and the antiviral DEGs. The lipidomic analysis showed that zebrafish gpbar1 activation in ZF4 cells resulted a change of glycerophospholipids, but none of bile acids nor their derivatives, which were different from mammalian GPBAR1. All together, these results firstly demonstrate the conserved antiviral role of gpbar1 and its function in regulating glycerophospholipids metabolism in teleost.
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http://dx.doi.org/10.1016/j.dci.2020.103955DOI Listing
March 2021

Evaluating the effects of ground-level O on rice yield and economic losses in Southern China.

Environ Pollut 2020 Dec 22;267:115694. Epub 2020 Sep 22.

Institute for Environmental and Climate Research, Jinan University, Guangzhou, China; Guangdong-Hongkong-Macau Joint Laboratory of Collaborative Innovation for Environmental Quality, Jinan University, Guangzhou, China; Research Center on Low-carbon Economy for Guangzhou Region, Jinan University, Guangzhou, China. Electronic address:

Ground-level ozone (O) pollution and its impact on crop growth and yield have become one of the serious environmental problems in recent years, especially in economically active and densely populated areas. In this study, rice yield and the associated economic losses due to O were estimated by using observational O concentration ([O]) data during growing seasons in Southern China. O-induced yield losses were calculated by using O exposure metrics of AOT40 and M7. The spatial distribution of these two metrics is relatively consistent, the highest areas located in the Yangtze River Basin. Under the current O level, during double-early rice, double-late rice and single rice growing seasons, the relative yield losses estimated with AOT40 (M7) were 6.8% (1.2%), 10.2% (1.9%) and 10.4% (2.0%), respectively. O-induced rice production loss for double-early rice, double-late rice and single rice totaled 2.4 million metric tons (0.4 million metric tons), 4.3 million metric tons (0.7 million metric tons) and 11.0 million metric tons (1.9 million metric tons) and associated economic losses were 108.1 million USD (18.3 million USD), 190.2 million USD (32.4 million USD) and 486.4 million USD (82.9 million USD) based on AOT40 (M7) metric. This study indicates that regional risks to rice from O exposure and provide quantitative evidence of O-induced impacts on rice yields and economic losses across Southern China. Therefore, the establishment of scientific O risk assessment method is of great significance to prevent yield production and economic losses caused by O exposure. Policymakers should strengthen supervision of emissions of O precursors to mitigate the rise of O concentration, thereby reducing O damage to agricultural production.
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http://dx.doi.org/10.1016/j.envpol.2020.115694DOI Listing
December 2020

Characterization and determination of free phytosterols and phytosterol conjugates: The potential phytochemicals to classify different rice bran oil and rice bran.

Food Chem 2021 May 12;344:128624. Epub 2020 Nov 12.

National Engineering Research Center for Functional Food, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China.

Phytosterols are important beneficial compounds found in rice bran (RB) and rice bran oil (RBO). Although relationships have been confirmed between the forms of phytosterols and their bioactivities, the analysis of different forms of phytosterols in RB and RBO has been lacking. In this study, high temperature gas chromatography-mass spectrometry (HTGC-MS) was combined with the single standard to determine multi-components (SSDMC) method to determine free sterols (FSs) and steryl glycosides (SGs) in RB and RBO. High-performance liquid chromatography (HPLC) was used to determine steryl ferulates (SFs). There was clear variation in the composition of FS, SF and SG, indicating that different forms of phytosterols can discriminate between different RB and RBO. The developed method may be also useful for the detection of other compounds of interest in oils, oil seeds or cereals.
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http://dx.doi.org/10.1016/j.foodchem.2020.128624DOI Listing
May 2021

Characterization of fatty acids, triacylglycerols, phytosterols and tocopherols in peony seed oil from five different major areas in China.

Food Res Int 2020 11 9;137:109416. Epub 2020 Jun 9.

Synergetic Innovation Center of Food Safety and Nutrition, State Key Laboratory of Food Science and Technology, International Joint Research Laboratory for Lipid Nutrition and Safety, School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, People's Republic of China. Electronic address:

Peony (Paeonia lactiflora pall) is a unique plant in China, which is planted on a large scale in different areas. The study characterized the triacylglycerol (TAG), fatty acid (FA), phytosterol, and tocopherol in peony seed oil (PSO) from five cultivation areas in China. The FAs in PSO were mainly C fatty acyl chains, especially α-linolenic acid (C). The triacylglycerol of PSO was identified by UPLC-Q-ToF/MS, and the predominant TAGs were LnLnLn, LLnLn, LLLn, LnLO/LLL, and OOLn/OLL. As for the micronutrients, γ-tocopherol and β-sitosterol were main components of tocopherol and phytosterol, respectively. The obtained data were further analyzed by principal components analysis (PCA), which allowed differentiation of five PSOs by their cultivation areas. For the great potential in unsaturated fatty acid and micronutrients, PSO could be a good dietary resource of plant oil.
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http://dx.doi.org/10.1016/j.foodres.2020.109416DOI Listing
November 2020

Identification and in vitro anti-inflammatory activity of different forms of phenolic compounds in Camellia oleifera oil.

Food Chem 2021 May 18;344:128660. Epub 2020 Nov 18.

School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, Jiangsu, China.

Camellia oleifera (C. oleifera) oil is known as "oriental olive oil". We previously reported the anti-inflammatory activity of C. oleifera oil was mainly attributed to the phenolic compounds, but the specific compounds remain uncovered. In this study, phenolic compounds in the form of free (11.92 μg GAE/g), esterified (37.57 μg GAE/g), glycosylated (128.71 μg GAE/g), and insoluble (47.53 μg GAE/g) were prepared from C. oleifera oil. Their anti-inflammatory activities were evaluated by lipopolysaccharide induced RAW 264.7 macrophage. Glycosylated fraction showed the highest anti-inflammatory activity as indicated by the low production of nitric oxide (NO), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6). Subsequently, 13 different glycosylated polyphenols were identified by UPLC-Q-TOF/MS, and the major compounds were purified for anti-inflammatory re-evaluation. Lower anti-inflammatory activities of compound 3 and compound 6 were observed when compared to kaempferol. Overall, these results would promote the utilization of phenolic compounds in C. oleifera oil.
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http://dx.doi.org/10.1016/j.foodchem.2020.128660DOI Listing
May 2021

Astaxanthin and its Effects in Inflammatory Responses and Inflammation-Associated Diseases: Recent Advances and Future Directions.

Molecules 2020 Nov 16;25(22). Epub 2020 Nov 16.

State Key Laboratory of Freshwater Ecology and Biotechnology, Key Laboratory of Aquaculture Disease Control, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.

Astaxanthin is a natural lipid-soluble and red-orange carotenoid. Due to its strong antioxidant property, anti-inflammatory, anti-apoptotic, and immune modulation, astaxanthin has gained growing interest as a multi-target pharmacological agent against various diseases. In the current review, the anti-inflammation mechanisms of astaxanthin involved in targeting for inflammatory biomarkers and multiple signaling pathways, including PI3K/AKT, Nrf2, NF-κB, ERK1/2, JNK, p38 MAPK, and JAK-2/STAT-3, have been described. Furthermore, the applications of anti-inflammatory effects of astaxanthin in neurological diseases, diabetes, gastrointestinal diseases, hepatic and renal diseases, eye and skin disorders, are highlighted. In addition to the protective effects of astaxanthin in various chronic and acute diseases, we also summarize recent advances for the inconsistent roles of astaxanthin in infectious diseases, and give our view that the exact function of astaxanthin in response to different pathogen infection and the potential protective effects of astaxanthin in viral infectious diseases should be important research directions in the future.
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http://dx.doi.org/10.3390/molecules25225342DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7696511PMC
November 2020

Antioxidant interaction of α-tocopherol, γ-oryzanol and phytosterol in rice bran oil.

Food Chem 2021 May 22;343:128431. Epub 2020 Oct 22.

National Engineering Research Center for Functional Food, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu PR China.

In this study, purified rice bran oil (RBO) was used as a lipid matrix model to study the individual and binary antioxidant capacity of the minor constituents (α-tocopherol, γ-oryzanol and phytosterol) added at different concentrations and ratios. The results revealed that concentration influenced on the oxidation stability and scavenging capacity, while ratio mainly affected the type of interaction or the degree of synergism or antagonism. It was important to notice that the antioxidant capacity of α-tocopherol would decrease under high concentration. Besides, the inhibition of phytosterol on α-tocopherol and the formation of hydrogen bond between γ-oryzanol and phytosterol were speculated by the interactions of these minor constituents. This work helps to select efficient combinations for stabilizing the anti-oxidation of nutrient enriched RBO or provide suggestions for moderate retain of minor constituents in RBO.
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http://dx.doi.org/10.1016/j.foodchem.2020.128431DOI Listing
May 2021

A Novel Transcript Isoform of TBK1 Negatively Regulates Type I IFN Production by Promoting Proteasomal Degradation of TBK1 and Lysosomal Degradation of IRF3.

Front Immunol 2020 30;11:580864. Epub 2020 Sep 30.

State Key Laboratory of Freshwater Ecology and Biotechnology, Key Laboratory of Aquaculture Disease Control, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China.

TANK-binding kinase 1 (TBK1), an IKK-related serine/threonine kinase, is pivotal for the induction of antiviral type I interferon (IFN) by TLR and RLR signaling pathways. In a previous study, we demonstrated that TBK1 spliced isoforms (TBK1_tv1 and TBK1_tv2) from zebrafish were dominant negative regulators in the RLR antiviral pathway by targeting the functional TBK1-IRF3 complex formation. In this study, we show that the third TBK1 isoform (namely TBK1_tv3) inhibits zebrafish type I IFN production by promoting TBK1 and IRF3 degradation. First, ectopic expression of TBK1_tv3 suppresses poly(I:C)- and Spring viremia of carp virus-induced type I IFN response, and also inhibits the up-regulation of IFN promoter activities stimulated by RIG-I, MDA5, MAVS, TBK1, and IRF3. Second, TBK1_tv3 targets TBK1 and IRF3 to impair the formation of TBK1 dimer, TBK1-IRF3 complex, and IRF3 dimer. Notably, TBK1_tv3 promotes the degradation of TBK1 through the ubiquitin-proteasome pathway and the degradation of IRF3 through the lysosomal pathway. Further analysis demonstrates that TBK1_tv3 promotes the degradation of TBK1 for K48-linked ubiquitination by targeting the K251, K256, and K271 sites of TBK1. Collectively, our results suggest a novel TBK1 isoform-mediated negative regulation mechanism, which serves to balance the production of type I IFN and ISGs.
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http://dx.doi.org/10.3389/fimmu.2020.580864DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7554342PMC
September 2020

IFRD1 regulates the asthmatic responses of airway via NF-κB pathway.

Mol Immunol 2020 11 29;127:186-192. Epub 2020 Sep 29.

Department of Pediatrics, An'kang Central Hospital, Ankang, 725000, Shaanxi, China. Electronic address:

Asthma is a chronic respiratory disease which is susceptible to children and causes great harm to them. Recently, Interferon-related developmental regulator 1 (IFRD1) was proved to be participant in regulating lung diseases, and its abnormal expression was shown in pathological airway tissues. Our study aimed to demonstrate the role and modulatory mechanism of IFRD1 in the pathogenesis of asthma. First, we evaluated the expression of IFRD1 in the lungs of asthmatic patients. C57BL/6 mice and human bronchial epithelioid (HBE) cells were respectively induced by ovalbumin (OVA) and lipopolysaccharide (LPS) to construct asthma models in vivo and in vitro. Using adenovirus and pcDNA vectors, we carried out overexpression assays on mice and cell models. Additionally, the potential mechanism of IFRD1 on regulating asthma process was elucidated by targeting NF-κB pathway. The results showed that IFRD1 was significantly down-regulated in asthma lung tissues, as well as the in vivo and in vitro models of asthma. Besides, OVA induced the inflammation responses and hyperreactivity of airway in mice, and LPS also caused inflammatory cytokine secretion and apoptosis of HBE cells, while cell viability was inhibited. However, IFRD1 overexpression dramatically reversed the effects of OVA and LPS. We subsequently discovered that the NF-κB pathway was activated in asthmatic cells, and NF-κB signaling activation was involved in IFRD1 regulated asthma responses of HBE cells. In conclusion, our study indicated that IFRD1 inhibited the asthmatic responses of airway via the NF-κB pathway inactivation. The evidence presented herein might provide a novel sight for asthma therapy.
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http://dx.doi.org/10.1016/j.molimm.2020.09.010DOI Listing
November 2020

Temporal and spatial patterns of nitrogen wet deposition in different weather types in the Pearl River Delta (PRD), China.

Sci Total Environ 2020 Oct 3;740:139936. Epub 2020 Jun 3.

Guangdong-Hongkong-Macau Joint Laboratory of Collaborative Innovation for Environmental Quality, Institute for Environmental and Climate Research, Jinan University, Guangzhou 510632, PR China. Electronic address:

The temporal and spatial patterns of nitrogen wet deposition were investigated in the Pearl River Delta (PRD) under different weather types. The study was carried out in 27 monitoring sites with reliable meteorological data from 2010 to 2017. Large spatial variation data showed that both annual volume weighted mean (VWM) concentrations and fluxes were higher in the central PRD while lower in the outer area. The annual mean concentrations and fluxes were in the range of 0.8-1.3 mg N L and 10.9-20.6 kg N ha yr, respectively. The monthly mean concentrations and fluxes ranged from 0.1 to 0.2 mg N L and 0.4 to 2.4 kg N ha, respectively. Further the study data revealed that the ratio of NH/NO was 1.1 which was much lower than the ratio reported in other regions like Northern China, Sichuan Basin, the US and Europe. The flux of NH in urban sites was comparable to rural sites, implying that potential non-agricultural NH emissions were likely to be high in the PRD. The top three weather types were E, C and SE, with the total contribution of more than a half to the flux. Multiple linear regression was used to set up an equation to predict the variation of annual fluxes under the changes in weather conditions. The result hints that the variation on annual fluxes in the PRD tends to be stable in the next 30 years. Considering the increasing impact on the ecosystem, more effort should be exerted to reduce nitrogen wet deposition in the future.
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http://dx.doi.org/10.1016/j.scitotenv.2020.139936DOI Listing
October 2020

Genetic Testing and Surveillance of Young Breast Cancer Survivors and Blood Relatives: A Cluster Randomized Trial.

Cancers (Basel) 2020 Sep 5;12(9). Epub 2020 Sep 5.

School of Public Health, University of Michigan, Ann Arbor, MI 48109-5618, USA.

We compared a tailored and a targeted intervention designed to increase genetic testing, clinical breast exam (CBE), and mammography in young breast cancer survivors (YBCS) (diagnosed <45 years old) and their blood relatives. A two-arm cluster randomized trial recruited a random sample of YBCS from the Michigan cancer registry and up to two of their blood relatives. Participants were stratified according to race and randomly assigned as family units to the tailored ( = 637) or the targeted ( = 595) intervention. Approximately 40% of participants were Black. Based on intention-to-treat analyses, YBCS in the tailored arm reported higher self-efficacy for genetic services ( = 0.0205) at 8-months follow-up. Genetic testing increased approximately 5% for YBCS in the tailored and the targeted arm ( ≤ 0.001; < 0.001) and for Black and White/Other YBCS ( < 0.001; < 0.001). CBEs and mammograms increased significantly in both arms, 5% for YBCS and 10% for relatives and were similar for Blacks and White/Others. YBCS and relatives needing less support from providers reported significantly higher self-efficacy and intention for genetic testing and surveillance. Black participants reported significantly higher satisfaction and acceptability. Effects of these two low-resource interventions were comparable to previous studies. Materials are suitable for Black women at risk for hereditary breast/ovarian cancer (HBOC).
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http://dx.doi.org/10.3390/cancers12092526DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7563571PMC
September 2020

The expanding and function of NLRC3 or NLRC3-like in teleost fish: Recent advances and novel insights.

Dev Comp Immunol 2021 Jan 5;114:103859. Epub 2020 Sep 5.

State Key Laboratory of Freshwater Ecology and Biotechnology, Key Laboratory of Aquaculture Disease Control, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei Province, 430072, China.

The nucleotide-binding domain and leucine-rich repeat-containing family (NLR) proteins are innate immune sensors which recognize highly conserved pathogen-associated molecular patterns (PAMPs). Mammals have small numbers of NLR proteins, whereas in some species such as in invertebrates and jawless vertebrates, NLRs have expanded into very large families. Nearly 400 NLR proteins are identified in the zebrafish genome. Members of the NLR family can be divided into two functional sub-groups based on their ability to either positively or negatively regulate host immune response or inflammatory signaling cascades. Mammalian NLRC3 has been identified as an inhibitory NLR, and serves as a negative regulator in the NF-κB-mediated inflammatory response, STING-mediated DNA sensing and PI3K-mTOR pathways. Different from mammalian NLRC3, the analysis from genomes or transcriptomes revealed that the expansions of NLRC3 existed in different species of fish. Furthermore, piscine NLRC3-like genes were confirmed to have a negative or positive regulatory function in response to different kinds of pathogen infections and in the production of proinflammatory cytokines. In this review, we summarize recent advances in our understanding of the expanding and function of NLRC3 or NLRC3-like genes in teleost fish, and give our view of important directions for future studies. The knowledge of piscine NLRC3 or expansive NLRC3-like genes-mediated biological functions in homeostasis and diseases will shed new light on the prevention and control of inflammatory and/or infectious diseases.
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http://dx.doi.org/10.1016/j.dci.2020.103859DOI Listing
January 2021

Time-resolved RNA-seq provided a new understanding of intestinal immune response of European eel (Anguilla anguilla) following infection with Aeromonas hydrophila.

Fish Shellfish Immunol 2020 Oct 21;105:297-309. Epub 2020 Jul 21.

State Key Laboratory of Freshwater Ecology and Biotechnology, Key Laboratory of Aquaculture Disease Control, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei Province, 430072, China; Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, China; University of Chinese Academy of Sciences, Beijing, China. Electronic address:

No studies systematically examined the intestinal immune response for yellow stage of European eel (Anguilla anguilla) with Aeromonas hydrophila infection by time-resolved RNA-seq. Here, we examined transcriptional profiles of the intestines at three-time points following infection with A. hydrophila. Intraperitoneal injections caused mortalities within 48 h post-injection (hpi), with the survival rate 87.5% at 24 hpi and 83.9% at 48 hpi. The result from KEGG pathway enrichment analysis showed that the immune related "cytosolic DNA-sensing pathway" was significantly enriched at the first and second time points (6 hpi and 18 hpi), with the up-regulated expression of irf3, il1b, tnfaip3, cxcl8a, ap1-2, c-fos, polr3d, polr3g and polr3k both at 6 hpi and 18 hpi, but not at the third time point (36 hpi). According to the KEGG annotation, 326 immune and inflammation-related DEGs were found. The co-expression network of those 326 DEGs revealed the existence of three modules, and tlr1 was found to be in the center of the biggest module which contained massive DEGs from "signal transduction" and "transport and catabolism". The c3 isoforms showed different expression pattern among the three time points, indicating a unique activation of complement systems at 18 hpi. Furthermore, two cathelicidins (aaCATH_1 and aaCATH_2) were highly up-regulated at the first two time points, and the bacterial growth inhibition assay revealed their antibacterial properties against A. hydrophila. Our data indicated the important roles of cytosolic DNA-sensing pathway, as well as transcripts including tlr1, c3, polr and cathelicidins in the intestine of A. anguilla in response to A. hydrophila infection. The present study will provide leads for functional studies of host-pathogen interactions.
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http://dx.doi.org/10.1016/j.fsi.2020.06.059DOI Listing
October 2020

Long non-coding RNA LINC00299 knockdown inhibits ox-LDL-induced T/G HAVSMC injury by regulating miR-135a-5p/XBP1 axis in atherosclerosis.

Panminerva Med 2020 Jul 22. Epub 2020 Jul 22.

Departmen of Cardiology, The First Affiliated Hospital of Qiqihar Medical College, Qiqihar, Heilongjiang, China.

Background: Atherosclerosis (AS) is a highly relevant social problem. Long noncoding RNA (lncRNA) long intergenic non-coding00299 (LINC00299) participates in the regulation of AS development. Therefore, this study was to explore the potential role and mechanism of LINC00299 in AS.

Methods: Human aortic vascular smooth muscle cells (T/G HA-VSMCs) were treated with oxidized low-density lipoprotein (ox-LDL). Cell viability and migration were measured by 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide (MTT) and transwell assays, severally. The activities of SOD and MDA were detected by total superoxide dismutase assay kit and malondialdehyde assay kit. The protein levels of ki67, matrix metalloproteinase 9 (MMP9) and X-box binding protein 1 (XBP1) were detected by western blot assay. The expression levels of LINC00299, microRNA-135a-5p (miR-135a-5p) and XBP1 were detected by real-time quantitative polymerase chain reaction (RT-qPCR). The binding relationship between miR-135a-5p and LINC00299 or XBP1 was predicted by miRcode and Starbase3.0 then verified by the dual-luciferase reporter assay.

Results: Ox-LDL induced cell viability, oxidative damage and migration of T/G HA-VSMCs. LINC00299 knockdown weakened ox-LDL-induced T/G HA-VSMCs injury. Mechanical analysis confirmed that LINC00299 improved XBP1 expression by interacting with miR-135a-5p. Furthermore, rescue assays showed that LINC00299 regulated ox-LDL-induced T/G HA-VSMCs injury through the miR-135a-5p/XBP1 axis.

Conclusions: Our studies revealed the regulatory function of LINC00299/miR-135a-5p/XBP1 axis in AS development, suggesting a potential therapeutic strategy for AS treatment.
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http://dx.doi.org/10.23736/S0031-0808.20.03942-7DOI Listing
July 2020

Safety, pharmacokinetics and causal prophylactic efficacy of KAF156 in a Plasmodium falciparum human infection study.

Clin Infect Dis 2020 Jul 9. Epub 2020 Jul 9.

Novartis Institutes for BioMedical Research, Emeryville, California, USA.

Background: KAF156 is a novel antimalarial drug that is active against both liver- and blood- stage Plasmodium parasites, including drug-resistant strains. Here, we investigated the causal prophylactic efficacy of KAF156 in a controlled human malaria infection (CHMI) model.

Methods: In Part 1, healthy, malaria-naïve participants received 800 mg KAF156 or placebo three hr before CHMI with Pf-infected mosquitoes. In Part 2, KAF156 was administered as single doses of 800, 300, 100, 50, or 20 mg 21 hr post-CHMI. All participants received atovaquone/proguanil treatment if blood-stage infection was detected or on day 29. For each cohort, 7-14 subjects were enrolled to KAF156 treatment and up to four subjects to placebo.

Results: KAF156 at all dose levels was safe and well tolerated. Two serious adverse events were reported - both resolved without sequelae and neither was considered related to KAF156. In Part 1, all participants treated with KAF156 and none of those randomized to placebo were protected against malaria infection. In Part 2, all participants treated with placebo or 20 mg KAF156 developed malaria infection. In contrast, 50 mg KAF156 protected 3/14 participants from infection, and doses of 800, 300, and 100 mg KAF156 protected all subjects against infection. An exposure-response analysis suggested that a 24-hr post-dose concentration of KAF156 of 21·5 ng/mL (90% CI 17.66 to 25.32 ng/mL) would ensure a 95% chance of protection from malaria parasite infection.

Conclusions: KAF156 was safe and well tolerated and demonstrated high levels of pre- and post-CHMI protective efficacy.
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http://dx.doi.org/10.1093/cid/ciaa952DOI Listing
July 2020

NLRC3-like 1 inhibits NOD1-RIPK2 pathway via targeting RIPK2.

Dev Comp Immunol 2020 11 4;112:103769. Epub 2020 Jul 4.

State Key Laboratory of Freshwater Ecology and Biotechnology, Key Laboratory of Aquaculture Disease Control, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei Province, 430072, China; Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, China; University of Chinese Academy of Sciences, Beijing, China. Electronic address:

Both NLRC3 and NOD1 belong to regulatory NLR subfamily based on their best-characterized function. In mammals, NLRC3 was reported to function by attenuating signaling cascades initiated by other families of PRRs. In teleosts, multiple NLRC3-like genes were identified through transcriptome sequencing. However, the functions of many NLRC3-like genes, especially the fish-specific NLRC3-like genes, remain unclear. In the present study, we report the functional characterization of a novel category of NLRC3-like proteins (named as NLRC3-like 1) from the zebrafish, which consists of a fish-specific FISNA, a conserved NACHT and five C-terminal LRRs domains. The expression of zebrafish NLRC3-like 1 was inducible in response to Edwardsiella piscicida infection. During bacterial infection, the in vitro and in vivo studies revealed that zebrafish NLRC3-like 1 overexpression facilitated bacterial growth and dissemination, together with the decreased survival rate of zebrafish larvae infected with E. piscicida. The attenuated response by zebrafish NLRC3-like 1 in response to bacterial infection were characterized by the impaired expression of antibacterial genes, proinflammatory cytokines and Nox genes. Furthermore, zebrafish NLRC3-like 1 interacted with the adaptor protein RIPK2 of NODs signaling via the FISNA (Fish-specific NACHT associated domain) and NACHT domains. However, the interaction between zebrafish NLRC3-like 1 and RIPK2 inhibited the assembly of the NOD1-RIPK2 complex. Importantly, zebrafish NLRC3-like 1 inhibited NOD1-mediated antibacterial activity, NF-κB and MAPK pathways and proinflammatory cytokine production. All together, these results firstly demonstrate that zebrafish NLRC3-like 1 inhibits NOD1-RIPK2 antibacterial pathway via targeting the adaptor protein RIPK2.
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http://dx.doi.org/10.1016/j.dci.2020.103769DOI Listing
November 2020