Publications by authors named "Min Zhou"

1,784 Publications

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Associations of polychlorinated biphenyls exposure with plasma glucose and diabetes in general Chinese population: The mediating effect of lipid peroxidation.

Environ Pollut 2022 Jun 20:119660. Epub 2022 Jun 20.

Department of Occupational & Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China; Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, and State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China. Electronic address:

Polychlorinated biphenyls (PCBs) exposure has been related to the abnormal glucose metabolism and the risk of diabetes. However, the joint effects of various PCBs are uncertain and the potential mechanisms remain unclear. Our objectives were to evaluate the associations of serum PCBs with fasting plasma glucose (FPG) and the risk of diabetes among a general Chinese population, and to estimate the mediating effects of oxidative stress in the above associations. Serum levels of seven indicator-PCBs (PCB-28, 52, 101, 118, 138, 153, and 180) and FPG values were determined among 4498 subjects from the Wuhan-Zhuhai cohort. Oxidative DNA damage biomarker (urinary 8-hydroxy-2'-deoxyguanosine, 8-OHdG) and lipid peroxidation biomarker (urinary 8-isoprostane, 8-iso-PGF) were also measured. Positive relationships of serum PCBs with FPG values as well as the risk of diabetes were observed. With each 1% increment in the natural log-transformed values of wet weight serum PCBs, FPG levels increased a 0.125% for PCB-52, 0.168% for PCB-118, 0.221% for PCB-138, 0.273% for PCB-153, and 0.379% for ΣPCB (the sum of seven PCBs). The adjusted odds ratios of diabetes associated with wet weight PCBs were 1.186 for PCB-52, 1.373 for PCB-118, 1.635 for PCB-153, and 1.456 for ΣPCB. The seven serum PCBs showed positive overall effect on the risk of diabetes. Elevated PCB-28, PCB-52, PCB-118, PCB-138, PCB-153, and ΣPCB were associated with the increased urinary 8-iso-PGF, which was positively related with FPG values. Furthermore, urinary 8-iso-PGF partially mediated the positive associations between PCBs and FPG values, with the mediated proportions ranged from 3.20 to 12.93%. In conclusion, our results suggested that serum PCBs were positively related with increased oxidative stress, FPG values, and the risk of diabetes among a general Chinese population. Serum PCBs mixture had positive overall effect on the risk of diabetes. Lipid peroxidation partly mediated the FPG elevation induced by PCB exposure.
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http://dx.doi.org/10.1016/j.envpol.2022.119660DOI Listing
June 2022

Biodegradable peptide polymers as alternatives to antibiotics used in aquaculture.

Biomater Sci 2022 Jun 22. Epub 2022 Jun 22.

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.

The pressure of antimicrobial resistance has forced many countries to reduce or even prohibit the use of antibiotics in feed. Therefore, it is an urgent need to develop alternatives to antibiotics to control infectious diseases in feed and aquaculture. To address this long-lasting challenge, we prepared peptide polymers that display potent and broad-spectrum activity against common pathogenic bacteria in aquaculture, low hemolysis and low cytotoxicity, and do not induce bacteria to develop resistance or cross-resistance to antibiotics. The optimal peptide polymer demonstrates strong therapeutic potential in an adult zebrafish infection model. Moreover, the optimal peptide polymer is biodegradable by enzymes into single amino acids and dipeptides to totally lose its antibacterial activity and, therefore, will not cause antimicrobial selective pressure. Our study suggests that peptide polymers are promising alternatives to antibiotics in aquaculture and open new avenues to address the global challenge of antimicrobial resistance.
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http://dx.doi.org/10.1039/d2bm00672cDOI Listing
June 2022

Genomic Characterization of Ciprofloxacin Resistance in Laboratory-Derived Mutants of .

Foodborne Pathog Dis 2022 Jun 21. Epub 2022 Jun 21.

Department of Food Quality and Safety, School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, China.

The quinolone ciprofloxacin is a broad-spectrum bactericidal antibiotic used for human medicine as well as the aquaculture industry. The emergence of ciprofloxacin-resistant strains is currently a global public health concern. However, the mechanism of ciprofloxacin resistance in is not yet fully clarified. We generated mutants with decreased ciprofloxacin susceptibility using selection and investigated genes associated with ciprofloxacin resistance on a genetic level. Our selection process yielded mutants that possessed altered minimal inhibitory concentrations (MICs) for ciprofloxacin and other unrelated antibiotics. These included Ser83Ile mutations in GyrA and Val461Glu in ParE as well as mutations in the resistance nodulation cell division (RND) family transporter gene and the putative TetR family regulator gene upstream of the operon. Measurements of steady-state mRNA levels revealed that the ciprofloxacin-resistant mutants overexpressed . Further, the introduction of the mutated allele from H512 into the sensitive parental strain increased the MIC for ciprofloxacin 31.25-fold. Taken together, these results indicated that ciprofloxacin resistance in these mutants was due to the quinolone resistance determining region mutation as well as overexpression of caused by a loss of gene repression. This also accounted for the presence of the multidrug resistance phenotype for these mutant strains since RND efflux system can export structurally unrelated antibiotics.
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http://dx.doi.org/10.1089/fpd.2022.0015DOI Listing
June 2022

[Distribution and bioactivity of polyphenols in Opuntia ficus-indica (L. ) Mill].

Wei Sheng Yan Jiu 2022 May;51(3):463-469

West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, China.

Objective: To study the distribution of the polyphenols of Opuntia ficus-indica(L. ) Mill, and study its antioxidant and the inhibition of sugar digestive enzyme activities by vitro experiments.

Methods: Using ethanol as solvent, free polyphenols, esterified polyphenols, and insoluble bonded polyphenols were extracted from the peel, pulp and seeds of Opuntia ficus-indica(L. ) Mill, and their contents were determined by the Folin-Ciocalteu method. Antioxidant activity was measured according to their ability to scavenge DPPH, OH and ABTS~(+ )radicals, and the inhibition of α-glucosidase and α-amylase activities by various types of polyphenols in various parts was observed in vitro.

Results: The peel polyphenol content of Opuntia ficus-indica(L. ) Mill was(1427.15±34.48) mg GAE/100 g, the pulp polyphenol content was(525.88±51.66) mg GAE/100 g, and the seed polyphenol content was(256.92±3.81) mg GAE/100 g. The free polyphenols, esterified polyphenols, and insoluble bonded polyphenols of different parts of Opuntia ficus-indica(L. ) Mill have the ability to scavenge DPPH, OH and ABTS~(+ )radicals, and the seeds had the strongest antioxidant capacity among all parts, the esterified polyphenols and insoluble bonded polyphenols of pulp and seeds of Opuntia ficus-indica(L. ) Mill have inhibitory effects on the activities of α-glucosidase and α-amylase, with seed polyphenols have the strongest inhibitory ability.

Conclusion: Opuntia ficus-indica(L. ) Mill has the highest content of polyphenols in the peel, and seed polyphenols have good antioxidant and the inhibition of sugar digestive enzyme activities.
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http://dx.doi.org/10.19813/j.cnki.weishengyanjiu.2022.03.020DOI Listing
May 2022

The prognostic effect on childhood acute lymphoblastic leukemia of CD34CD38 expressed in leukemia cells.

Hematology 2022 Dec;27(1):706-713

Department of Pediatrics, Huai'an First Hospital Affiliated to Nanjing Medical University, Jiangsu, People's Republic of China.

Objective: Acute lymphoblastic leukemia is the most common malignant disease in children. CD34 and CD38 are expressed in both normal and leukemia cells, but studies of their prognostic associations in childhood acute lymphoblastic leukemia are limited. The aim of this study was to investigate the prognostic effect of CD34 CD38 leukemia cells in this childhood cancer.

Methods: From January 2014 to January 2019, children with newly diagnosed acute lymphoblastic leukemia were included in this study and followed up until July 2020. The participants were divided into CD34 and CD34 groups according to CD34 expression level at diagnosis, and the CD34 group was further divided into CD34 CD38 and CD34 CD38 subgroups based on CD38 expression level. We tracked clinical biological features, therapeutic outcomes, and other patient data for comparisons.

Results: The OS and EFS did not differ significantly between the CD34 and CD34 groups (both > 0.05). CD34CD38 group and CD34CD38 group were further compared. OS differed significantly between these two groups (χ= 3.89, = 0.048), as did the recurrence rate (χ= 5.04, = 0.025), but EFS did not (χ= 1.45, > 0.05). Survival analysis in patients with recurrence showed a significantly higher OS for the CD34 CD38 group compared with the CD34 CD38 group (χ= 5.08, = 0.024). The CD34CD38 group and CD34CD38 group were matched for propensity scores. When recurrence was compared in the two groups after matching, the difference was statistically significant (< 0.001).

Conclusion: CD34 and CD34 expression does not differ by prognosis in children with acute lymphoblastic leukemia, but CD34 CD38 may indicate a poor prognosis.
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http://dx.doi.org/10.1080/16078454.2022.2080368DOI Listing
December 2022

Freeze-thaw alternations accelerate plasticizers release and pose a risk for exposed organisms.

Ecotoxicol Environ Saf 2022 Jun 6;241:113742. Epub 2022 Jun 6.

Key Laboratory of the Ministry of Education for the Ecological Environment of the Three Gorges Reservoir Area, Chongqing University, Chongqing 400030, China; Institute for Environmental Research, RWTH Aachen University, Aachen 52074, Germany; State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210093, China.

The application of plastic mulch films brings convenience to agricultural production, but also causes plastic waste that can be degraded into microplastics (MPs). However, little is known about the fate of plastic waste in agricultural ecosystem under freeze-thaw alternation in middle and high latitudes, as well as in highlands around the world. Whether the release of plasticizers, i.e. phthalate esters (PAEs), under such conditions would pose a potential risk to exposed organisms due to bioaccumulation is also unknown. To fill these data gaps, the agricultural fields in Liaoning of China with typical freeze-thaw alternation was selected as the study area. The transformation of plastic film was demonstrated by simulation freeze-thaw alternating from -30 to 20 ℃. Soil samples were collected to investigate the patterns of MP composition, abundance, and distribution. Concurrently, the concentrations of two PAEs including bis(2-ethylhexyl) phthalate (DEHP) and diethyl phthalate (DEP) in soils were analyzed to provide information on the correlation between MPs abundance and PAEs concentrations as well as potential risks. The results showed that freeze-thaw alternating can accelerate the formation of MPs and release of PAEs from plastic waste. The abundance of MPs was positively correlated with the concentration of PAEs. Soil PAEs ranged from 3268 ± 213-6351 ± 110 μg/kg, indicating that over 40 % of the PAEs were transferred from plastic films to soils. Such residual amounts could pose risk for exposed organisms. Hence, the current study suggested that special concerns should be given to the release plasticizers in plastic waste of agricultural soils.
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http://dx.doi.org/10.1016/j.ecoenv.2022.113742DOI Listing
June 2022

Bacteriostatic effects of phage F23s1 and its endolysin on Vibrio parahaemolyticus.

J Basic Microbiol 2022 Jun 3. Epub 2022 Jun 3.

Department of Food Quality and Safety, School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, China.

Vibrio parahaemolyticus is a common foodborne pathogenic bacterium and drug-resistant strains are now widespread. Phages led by drug-resistant V. parahaemolyticus strains are promising means to decrease the pressure on public health. We isolated a V. parahaemolyticus-specific bacteriophage F23s1 that was active at wide ranges of temperature (30-60°C) and pH (4-10). Phage F23s1 exhibited a specific host range; in that, only 13 of the 23 V. parahaemolyticus strains were lysed. F23s1 effectively inhibited the growth of V. parahaemolyticus strain F23 in shrimp at 25°C within 12 h at a multiplicity of infection of 1000. We sequenced the genome of phage F23s1 which comprised a 76,648-bp DNA with 105 open reading frames (ORFs) and identified an endolysin gene ORF52 that was then cloned and successfully expressed in Escherichia coli. The recombinant ORF52 protein significantly decreased OD of V. parahaemolyticus F23 from 0.978 to 0.249 when used at 20 µmol/L within 60 min. The endolysin also showed lytic activity against a panel of 23 drug-resistant V. parahaemolyticus and 12 Salmonella strains with a higher lytic ability for V. parahaemolyticus. The phage F23s1 and its endolysin will be useful for preventing and controlling V. parahaemolyticus in food safety.
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http://dx.doi.org/10.1002/jobm.202200056DOI Listing
June 2022

Oxygen Vacancy Induced Boosted Visible-Light Driven Photocatalytic CO Reduction and Electrochemical Water Oxidation Over [email protected] O @CC Architecture.

Small Methods 2022 Jun 3:e2200308. Epub 2022 Jun 3.

Hefei National Laboratory for Physical Science at the Microscale, Department of Applied Chemistry, University of Science and Technology of China, Hefei, Anhui, 230026, P. R. China.

Herein, the obtained Cu Co [email protected] O @CC-150 heterojunction (termed as Cu Co -ZFC-150) showed high hydrogen and oxygen evolution reaction (HER and OER) activities with low overpotential small Tafel slope. When employed to be the bifunctional anode and cathode, they only needed a cell voltage of 1.62 V. The composite also exhibited excellent photocatalytic performance on CO evolution into CO and CH . The enhanced OER kinetics and Z-scheme charge transfer model for photocatalytic property have been discussed based on the experiments and density functional theory (DFT) analysis. The optimized phase interfaces, abundant active sites, optional oxygen vacancy, and adjusted Gibbs free energy were beneficial for the fast electron/ion transport enhancing the water splitting performance.
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http://dx.doi.org/10.1002/smtd.202200308DOI Listing
June 2022

Five-Year Results of Aortic Remodeling for Acute, Subacute, and Chronic Type B Aortic Dissection Following Endovascular Repair.

Front Cardiovasc Med 2022 17;9:847368. Epub 2022 May 17.

Department of Vascular Surgery, Drum Tower Hospital, Affiliated to School of Medicine, Nanjing University, Nanjing, China.

Background: This study was performed to compare aortic remodeling and clinical outcomes in patients with acute, subacute, and chronic type B aortic dissection (TBAD) after thoracic endovascular aortic repair (TEVAR).

Methods: We retrospectively examined 323 consecutive patients with acute ( = 129), subacute ( = 161), and chronic ( = 33) TBAD who underwent TEVAR from June 2013 to December 2016 in in multicenter institution. Patient demographics, clinical data, and imaging characteristics were recorded and compared among the three groups.

Results: The three groups had comparable baseline characteristics. Perioperative mortality rates were similar among the acute (2.3%), subacute (0.0%), and chronic (0.0%) groups ( = 0.34). Perioperative morbidity rates, including the rates of visceral and lower limb malperfusion and cerebral infraction, were also similar. The rate of perioperative endoleak was significantly higher in the chronic group (18.1%) than in the acute (3.9%) and subacute (3.7%) groups ( = 0.02). The mean follow-up duration was 78 ± 22 months (range, 36-101 months). The mortality rates were comparable among the three groups. The rates of reintervention and lower limb malperfusion were higher in the chronic group than in the acute and subacute groups. FL diameter reduction were more robust in the acute and subacute groups than in the chronic group.

Conclusion: Patients with acute, subacute, and chronic TBAD had different outcomes in this study. Patients with acute and subacute TBAD had fewer complications than those with chronic TBAD. Aortic remodeling after TEVAR was more favorable in patients with acute and subacute TBAD than in patients with chronic TBAD. TEVAR promotes more positive remodeling at the stent graft level than at the distal level of the aorta.
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http://dx.doi.org/10.3389/fcvm.2022.847368DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9152007PMC
May 2022

An Admittance-Controlled Amplified Force Tracking Scheme for Collaborative Lumbar Puncture Surgical Robot System.

Int J Med Robot 2022 Jun 1:e2428. Epub 2022 Jun 1.

Higher Institute of Information Technology and Information Systems, Kazan, Russian Federation.

Background: The accurate sensing and display of the delicate needle-tissue interaction force to the operator is desirable for needle insertion procedures. It not only plays a significant role in the surgical treatment effect, but also has a great significance in improving surgical safety and reducing the incidence of complications. However, the direct detection of the interaction force between the tissue and needle tip by placement of a force sensor is challenging owing to the constraints of miniaturization, cost, and sterilization.

Methods: In this study, a new position-based force-to-motion controller with magnified force feedback is presented to provide augmented force perception to the operator during needle insertion on the soft tissue. Furthermore, the demonstration to the position-based low level motion controller is more suitable for needle insertion surgical requirements in the cooperative robotic system.

Results: The proposed controller was experimentally validated by a collaborative lumbar puncture robotics system. Additionally, to provide hand tremor rejection for the stable manipulation of the puncture needle, it was demonstrated that the proposed amplified feedback force controller allowed a safer object interaction with the robotic needle insertion assistance.

Conclusions: The results of the experiment show that a desirable interaction force profile is perceived by the operator during the overall insertion task operation. The admittance gain for the simplified admittance controller has a significant impact on the operator's ability to accurately control the applied force. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/rcs.2428DOI Listing
June 2022

[Lower Gastrointestinal Bleeding: Current State of Diagnosis and Treatment and Interpretation of the Clinical Guidelines].

Sichuan Da Xue Xue Bao Yi Xue Ban 2022 May;53(3):367-374

Department of Gastroenterology, West China Hospital, Sichuan University, Chengdu 610041, China.

Lower gastrointestinal bleeding (LGIB) is a common clinical emergency. However, most of the published findings on LGIB were of retrospective or observational studies, and the relevant clinical guidelines and consensuses were not published until quite recently. In clinical practice, LGIB treatment is not as standardized as the treatment of upper gastrointestinal bleeding. Herein, on the basis of the latest clinical research findings on and guidelines for LGIB, we summarized and analyzed the existent diagnosis and treatment of LGIB from the perspectives of patient assessment and endoscopic, interventional and medication treatment, intending to provide more references to support the clinical practice.
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http://dx.doi.org/10.12182/20220560207DOI Listing
May 2022

Influence of rigid wall on the nonlinear pulsation of nearby bubble.

Ultrason Sonochem 2022 Jun 13;87:106034. Epub 2022 May 13.

Key Laboratory of Modern Acoustics, Ministry of Education, Institute of Acoustics, Nanjing University, Nanjing 210093, China.

This paper mainly focuses on the nonlinear pulsation of a bubble near the rigid wall. Dynamics of near-wall bubble and free bubble are discussed and compared in details. Investigation reveals as the driving acoustic pressure amplitude increases, nonlinear pulsation of bubble becomes intense gradually. Besides, decreasing the viscosity of host liquid is advantageous for the nonlinear pulsation of bubble. Bifurcation diagrams of bubble radius show acoustic reflection of the rigid wall makes the initial bifurcation appear at low driving acoustic amplitude and on bubble with small ambient radius, and makes the bifurcation still exist for bubble in high-viscosity liquids. That indicates the rigid wall will produce enhancement on the nonlinearity of nearby bubble. As the bubble approaches the wall, the enhancement becomes strong. Moreover, research on the influence of driving frequency shows the rigid wall makes the frequency band corresponding to chaos around the resonant frequency of free bubble shift downward.
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http://dx.doi.org/10.1016/j.ultsonch.2022.106034DOI Listing
June 2022

A live attenuated virus-based intranasal COVID-19 vaccine provides rapid, prolonged, and broad protection against SARS-CoV-2.

Sci Bull (Beijing) 2022 May 26. Epub 2022 May 26.

State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Life Sciences, School of Public Health, Xiamen University, Xiamen 361104, China.

Remarkable progress has been made in developing intramuscular vaccines against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2); however, they are limited with respect to eliciting local immunity in the respiratory tract, which is the primary infection site for SARS-CoV-2. To overcome the limitations of intramuscular vaccines, we constructed a nasal vaccine candidate based on an influenza vector by inserting a gene encoding the receptor-binding domain (RBD) of the spike protein of SARS-CoV-2, named CA4-dNS1-nCoV-RBD (dNS1-RBD). A preclinical study showed that in hamsters challenged 1 d after single-dose vaccination or 9 months after booster vaccination, dNS1-RBD largely mitigated lung pathology, with no loss of body weight. Moreover, such cellular immunity is relatively unimpaired for the most concerning SARS-CoV-2 variants, especially for the latest Omicron variant. In addition, this vaccine also provides cross-protection against H1N1 and H5N1 influenza viruses. The protective immune mechanism of dNS1-RBD could be attributed to the innate immune response in the nasal epithelium, local RBD-specific T cell response in the lung, and RBD-specific IgA and IgG response. Thus, this study demonstrates that the intranasally delivered dNS1-RBD vaccine candidate may offer an important addition to the fight against the ongoing coronavirus disease 2019 pandemic and influenza infection, compensating limitations of current intramuscular vaccines.
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http://dx.doi.org/10.1016/j.scib.2022.05.018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9134758PMC
May 2022

GLP-1 regulates exercise endurance and skeletal muscle remodeling via GLP-1R/AMPK pathway.

Biochim Biophys Acta Mol Cell Res 2022 09 27;1869(9):119300. Epub 2022 May 27.

Research Center for Nutrition and Food Safety, Chongqing Key Laboratory of Nutrition and Food Safety, Institute of Military Preventive Medicine, Third Military Medical University (Army Medical University), NO. 30th Gao Tan Yan Street, Shapingba District, Chongqing 400038, People's Republic of China. Electronic address:

Exercise-induced physical endurance enhancement and skeletal muscle remodeling can prevent and delay the development of multiple diseases, especially metabolic syndrome. Herein, the study explored the association between glucagon-like peptide-1 (GLP-1) secretion and exercise, and its effect on skeletal muscle remodeling to enhance endurance capacity. We found both acute exercise and short-term endurance training significantly increased the secretion of GLP-1 in mice. Recombinant adeno-associated virus (AAV) encoding Gcg (proglucagon) was used to induce the overexpression of GLP-1 in skeletal muscle of mice. Overexpression of GLP-1 in skeletal muscle enhanced endurance capacity. Meanwhile, glycogen synthesis, glucose uptake, type I fibers proportion, and mitochondrial biogenesis were augmented in GLP-1-AAV skeletal muscle. Furthermore, the in vitro experiment showed that exendin-4 (a GLP-1 receptor agonist) treatment remarkably promoted glucose uptake, type I fibers formation, and mitochondrial respiration. Mechanistically, the knockdown of AMPK could reverse the effects imposed by GLP-1R activation in vitro. Taken together, these results verify that GLP-1 regulates skeletal muscle remodeling to enhance exercise endurance possibly via GLP-1R signaling-mediated phosphorylation of AMPK.
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http://dx.doi.org/10.1016/j.bbamcr.2022.119300DOI Listing
September 2022

Indole Alkaloids and Chromones from the Stem Bark of and Their Antiviral Activities.

Molecules 2022 May 13;27(10). Epub 2022 May 13.

Key Laboratory of Chemistry in Ethnic Medicinal Resources, State Ethnic Affairs Commission & Ministry of Education, Yunnan Minzu University, Kunming 650031, China.

The (Leguminosae) genus has attracted a lot of attention as a prolific source of alkaloids and chromones with diverse structures and biological properties. The aim of this study is to screen the antiviral compounds from . The extract of the stem bark of this plant was separated using silica gel, MCI, ODS C18, and Sephadex LH-20 column chromatography, as well as semi-preparative HPLC. As a result, three new indole alkaloids, alataindoleins A-C (1-3); one new chromone, alatachromone A (4); and a new dimeric chromone-indole alkaloid, alataindolein D (5) were isolated. Their structures were determined by means of HRESIMS and extensive 1D and 2D NMR spectroscopic studies. Interestingly, alataindolein D (5) represents a new type of dimeric alkaloid with an unusual N-2-C-16' linkage, which is biogenetically derived from a chromone and an indole alkaloid via an intermolecular nucleophilic substitution reaction. Compounds 1-5 were tested for their anti-tobacco mosaic virus (TMV) and anti-rotavirus activities, and the results showed that compounds 2-4 showed high anti-TMV activities with inhibition rates of 44.4%, 66.5%, and 52.3%, respectively. These rates were higher than those of the positive control (with inhibition rate of 32.8%). Compounds 1 and 5 also showed potential anti-TMV activities with inhibition rates of 26.5% and 31.8%, respectively. In addition, compounds 1-5 exhibited potential anti-rotavirus activities with therapeutic index (TI) values in the range of 9.75~15.3. The successful isolation and structure identification of the above new compounds provided materials for the screening of antivirus drugs, and contributed to the development and utilization of .
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http://dx.doi.org/10.3390/molecules27103129DOI Listing
May 2022

Compute-in-Memory for Numerical Computations.

Micromachines (Basel) 2022 May 2;13(5). Epub 2022 May 2.

State Key Laboratory of ASIC and System, School of Microelectronics, Fudan University, Shanghai 201203, China.

In recent years, compute-in-memory (CIM) has been extensively studied to improve the energy efficiency of computing by reducing data movement. At present, CIM is frequently used in data-intensive computing. Data-intensive computing applications, such as all kinds of neural networks (NNs) in machine learning (ML), are regarded as 'soft' computing tasks. The 'soft' computing tasks are computations that can tolerate low computing precision with little accuracy degradation. However, 'hard' tasks aimed at numerical computations require high-precision computing and are also accompanied by energy efficiency problems. Numerical computations exist in lots of applications, including partial differential equations (PDEs) and large-scale matrix multiplication. Therefore, it is necessary to study CIM for numerical computations. This article reviews the recent developments of CIM for numerical computations. The different kinds of numerical methods solving partial differential equations and the transformation of matrixes are deduced in detail. This paper also discusses the iterative computation of a large-scale matrix, which tremendously affects the efficiency of numerical computations. The working procedure of the ReRAM-based partial differential equation solver is emphatically introduced. Moreover, other PDEs solvers, and other research about CIM for numerical computations, are also summarized. Finally, prospects and the future of CIM for numerical computations with high accuracy are discussed.
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http://dx.doi.org/10.3390/mi13050731DOI Listing
May 2022

Experimental Study on Mechanical Properties of Root-Soil Composite Reinforced by MICP.

Materials (Basel) 2022 May 17;15(10). Epub 2022 May 17.

School of Civil Engineering and Architecture, Chongqing University of Science and Technology, Chongqing 401331, China.

Mechanical properties of undisturbed root-soil composites were investigated through direct shear tests under different cementation concentrations by microbially induced carbonate precipitation (MICP). The results show that MICP has a significant strengthening effect on the undisturbed root-soil composite, and the maximum shear strength increases by about 160% after grouting. The shear strength of root-soil composites increases with the increase in calcium chloride concentration, and the shear strength increases the most when the concentration is 0.75M. Calcium carbonate formed by MICP treatment has cementitious properties, which increases the cohesion and internal friction angle of the root-soil composite by about 400% and 120%, respectively. The results show that it is feasible to solidify slope and control soil erosion together with microbial and vegetation roots. The research results can serve as a scientific basis and reference for the application of MICP technology in vegetation slope protection engineering.
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http://dx.doi.org/10.3390/ma15103586DOI Listing
May 2022

Acid rain-dependent detailed leaching characteristics and simultaneous immobilization of Pb, Zn, Cr, and Cd from hazardous lead-zinc tailing.

Environ Pollut 2022 Aug 24;307:119529. Epub 2022 May 24.

School of Resource and Environment Science, Wuhan University, Wuhan, 430072, PR China; Hubei Environmental Remediation Material Engineering Technology Research Center, Wuhan, 430072, PR China. Electronic address:

In acidic medium, hazardous heavy metals of lead-zinc tailing (LZT) are easily leachable and mobilizable. Thus, the hazard, amount, form, and complexity of the leached heavy metals under acidic precipitation become a major environmental concern. This work investigates the gangue minerals, toxicity, speciation, leaching characteristics of heavy metals in LZT under simulated acid rain, as well as immobilization effects and mechanisms using a sustainable binder. In LZT, dolomite, quartz, calcite, and muscovite are the main gangue minerals, tiny hazardous metallic minerals were absorbed in the surface. The results revealed that Pb, Zn, Cr, and Cd were the predominant harmful elements, particularly Pb and Zn. Zn is leached completely and is the concerned hazardous element under simulated acid rain. In the acid rain neutralization ability test, the amount of leachable Pb, Cr, Ca, and Si maintained in equilibrium, leached Zn, Cd, Al, and Mg depended on the addition of acid. Pb and Ca were sedimented in residues. Immobilization of Pb, Zn, Cr, and Cd depended on the stability of Ca(OH)/C-S-H of hydrates, and 70% LZTHP after curing 7 days can be used for some practical engineering projects. This work opens up deeply understandings for the leached heavy metals under acidic precipitation and improves the sustainable and safe in the field of immobilization of heavy metals.
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http://dx.doi.org/10.1016/j.envpol.2022.119529DOI Listing
August 2022

CF Plasma-Generated LiF-Li C Artificial Layers for Dendrite-Free Lithium-Metal Anodes.

Adv Sci (Weinh) 2022 May 26:e2201147. Epub 2022 May 26.

State Key Laboratory of Advanced Electromagnetic Engineering and Technology, School of Electrical and Electronic Engineering, Huazhong University of Science and Technology, Wuhan, 430074, China.

Lithium metal anodes have long been considered as "holy grail" in the field of energy storage batteries, but dendrite growth and large volume changes hinder their practical applications. Herein, a facile and eco-friendly CF plasma treatment is employed for the surface modification of Li anodes, and an artificial layer consisting of LiF and Li C is fabricated for the first time. Experimental results and theoretical calculations reveal that the high adsorption energy of LiF and low Li diffusion barriers in Li C induce uniform nucleation and planar growth of Li, guaranteeing a stable and dendrite-free Li structure during the repeated plating/stripping process of cycling. Symmetric cells using CF plasma-treated Li operate stably for more than 6500 h (at 2 mA cm and 1 mAh cm ) or 950 h (at 1 mA cm and 10 mAh cm ). When paired with a LiFePO cathode, full batteries deliver a high reversible capacity of 136 mAh g (at 1 C) with considerable cycling stability (97.2% capacity retention over 200 cycles) and rate performance (116 mAh g up to 5 C). This powerful application of plasma technology toward novel LiF-Li C artificial layers provide new routes for constructing environment-friendly and high-performance energy storage devices.
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http://dx.doi.org/10.1002/advs.202201147DOI Listing
May 2022

A 2-bp deletion mutation in gene leading to lysosomal acid sphingomyelinase deficiency in a Chinese consanguineous pedigree.

J Pediatr Endocrinol Metab 2022 May 27. Epub 2022 May 27.

Prenatal Diagnosis Department, Chengdu Women's and Children's Central Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China.

Background: Niemann-Pick disease type A (NPDA, MIM: 257200) is an autosomal recessive sphingolipidosis caused by lysosomal acid sphingomyelinase (ASM) deficiency. A cluster of genes located at chromosome 11p15 have been reported to be imprinted genes, such as TSSC5, TSSC3, and ZNF215 that flanking SMPD1 gene. It was reported by a few recent studies that SMPD1 gene was paternally imprinted and maternally preferentially expressed.

Case Presentation: A five-month-old boy with severe anemia, hepatosplenomegly and bone marrow foam cells was recruited from a complete cousin couple. To determine whether boy suffered from NPDA, ASM activity and SMPD1 gene sequencing were performed on available individuals of this pedigree including the proband, his parents and sister. The ASM activities of proband and parents showed deficiency (17.7 nmol/h/g-protein) and about 50% decreased (83.3 nmol/h/g-protein), respectively, compared with normal controls (204.5 nmol/h/g-protein). SMPD1 gene sequencing in the proband revealed a homozygous mutation c.1420_1421del, which leads to an open reading frameshift and a premature stop codon. The parents and some individuals of this family demonstrated heterozygous mutation at this locus. To investigate whether SMPD1 gene is imprinted as reported previously, the expression of RNA level was studied in the whole family members available. The members with heterozygous mutation for c.1420_1421del showed that both paternal and maternal inherited alleles were expressed.

Conclusions: This study reported a c.1420_1421del mutation in SMPD1 gene which caused ASM activity decrease and this locus was biallelically expressed in heterozygous subjects implicating SMPD1 is not imprinted in this family.
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http://dx.doi.org/10.1515/jpem-2021-0480DOI Listing
May 2022

SDG-oriented multi-scenario sustainable land-use simulation under the background of urban expansion.

Environ Sci Pollut Res Int 2022 May 25. Epub 2022 May 25.

College of Public Administration, Huazhong University of Science and Technology, Hongshan District, Number 1037, Luoyu Road, Wuhan, China.

With the continuous improvement of urbanization level and the continuous expansion of city scale, there are some unreasonable land development and utilization problems, which will make the contradiction between people and land more prominent and the risk of ecological environment deterioration more serious. It hinders the sustainable land use (SLU) and then affects the realization of regional Sustainable Development Goals (SDGs). SDGs have become a programmatic document for all countries in the world to implement sustainable development. It provides a guideline and direction for the sustainable urban expansion. The sustainable urban expansion promotes the realization of SDGs worldwide. By combining the SDGs with the urban SLU, this paper obtains the optimized future land-use demand of the multi-scenario city and the urban expansion simulation scenario to the SDGs under the multi-scenario through the uncertain mathematical model (MIFCCP) and the spatial simulation model (PLUS). We find that firstly, the net profit of land use (LNB) reaches the highest value under the economic development scenario (ED), when the probability of environmental constraint violation p = 0.01 and p = 0.15, LNB = [2625.48, 3244.98] × 10 CNY, and [2646.95, 3271.51] × 10 CNY. Ecosystem service value (ESV) reached the highest value under the sustainable development scenario (SD), when p = 0.01 and p = 0.15, ESV = [75.34, 93.12] × 10 CNY, and [72.62, 95.56] × 10 CNY. The net carbon emissions from land use (LNC) reached the minimum value in SD scenario, and when p = 0.01 and p = 0.15, the LNC reached [57.46, 71.02] × 10 ton and [56.12, 76.04] × 10 ton. Secondly, the contribution degree of 15 driving factors to the change of local types is excavated, among which, the driving factors of traffic stations have the highest contribution degree to the change of construction land and cultivated land, and the third-class roads have the highest contribution degree to the change of wetland area. Furthermore, by analyzing the indicators related to SLU in SDGs, we can build an optimization model of land use quantity structure under uncertain conditions, and the optimized results can meet the targets of economic benefits, ecological benefits, and net carbon emissions of land use under different development plans. By linking SDGs and SLU, the coupled model framework can provide scientific basis for urban land expansion strategy based on ecological environment constraints and scientific support for sustainable management of land use.
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http://dx.doi.org/10.1007/s11356-022-20904-9DOI Listing
May 2022

Correction: LncRNA AFAP1-AS1 promotes growth and metastasis of cholangiocarcinoma cells.

Oncotarget 2022 19;13:694. Epub 2022 May 19.

Department of Biliary-Pancreatic Surgery, Affiliated Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

[This corrects the article DOI: 10.18632/oncotarget.16880.].
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http://dx.doi.org/10.18632/oncotarget.28216DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9119686PMC
May 2022

BMAL1 modulates smooth muscle cells phenotypic switch towards fibroblast-like cells and stabilizes atherosclerotic plaques by upregulating YAP1.

Biochim Biophys Acta Mol Basis Dis 2022 Sep 20;1868(9):166450. Epub 2022 May 20.

Department of Vascular Surgery, Institute of Vascular Surgery, National Clinical Research Center for Interventional Medicine, Zhongshan Hospital, Fudan University, 180 Fenglin Rd., Shanghai 200032, China. Electronic address:

Background: Ischemic heart diseases and ischemic stroke are closely related to circadian clock and unstable atherosclerotic plaques. Vascular smooth muscle cells (VSMCs) can stabilize or destabilize an atherosclerotic lesion through phenotypic switch. BMAL1 is not only an indispensable core component in circadian clock but also an important regulator in atherosclerosis and VSMCs proliferation. However, little is known about the modulation mechanisms of BMAL1 in VSMCs phenotypic switch and atherosclerotic plaque stability.

Methods: We integrated histological analysis of human plaques, in vivo experiments of VSMC-specific Bmal1 mice, in vitro experiments, and gene set enrichment analysis (GSEA) of public datasets of human plaques to explore the function of BMAL1 in VSMCs phonotypic switch and plaque stability.

Findings: Comparing to human unstable plaques, BMAL1 was higher in stable plaques, accompanied by elevated YAP1 and fibroblast maker FSP1 which were positively correlated with BMAL1. In response to Methyl-β-cyclodextrin-cholesterol, oxidized-low-density-lipoprotein and platelet-derived-growth-factor-BB, VSMCs embarked on phenotypic switch and upregulated BMAL, YAP1 and FSP1. Besides, BMAL1 overexpression promoted VSMCs phonotypic switch towards fibroblast-like cells by transcriptionally upregulating the expression of YAP1. BMAL1 or YAP1 knock-down inhibited VSMCs phonotypic switch and downregulated FSP1. Furthermore, VSMC-specific Bmal1 mice exhibited VSMCs with lower YAP1 and FSP1 levels, and more vulnerable plaques with less collagen content. In addition, BMAL1 suppressed the migration of VSMCs. The GSEA results of public datasets were consistent with our laboratory findings.

Interpretation: Our results highlight the importance of BMAL1 as a major regulator in VSMCs phenotypic switch towards fibroblast-like cells which stabilize an atherosclerotic plaque.
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http://dx.doi.org/10.1016/j.bbadis.2022.166450DOI Listing
September 2022

Post-Stroke Administration of L-4F Promotes Neurovascular and White Matter Remodeling in Type-2 Diabetic Stroke Mice.

Front Neurol 2022 28;13:863934. Epub 2022 Apr 28.

Department of Neurology, Henry Ford Hospital, Detroit, MI, United States.

Patients with type 2 diabetes mellitus (T2DM) exhibit a distinct and high risk of ischemic stroke with worse post-stroke neurovascular and white matter (WM) prognosis than the non-diabetic population. In the central nervous system, the ATP-binding cassette transporter member A 1 (ABCA1), a reverse cholesterol transporter that efflux cellular cholesterol, plays an important role in high-density lipoprotein (HDL) biogenesis and in maintaining neurovascular stability and WM integrity. Our previous study shows that L-4F, an economical apolipoprotein A member I (ApoA-I) mimetic peptide, has neuroprotective effects alleviating neurovascular and WM impairments in the brain of db/db-T2DM stroke mice. To further investigate whether L-4F has neurorestorative benefits in the ischemic brain after stroke in T2DM and elucidate the underlying molecular mechanisms, we subjected middle-aged, brain-ABCA1 deficient (ABCA1), and ABCA1-floxed (ABCA1) T2DM control mice to distal middle cerebral artery occlusion. L-4F (16 mg/kg, subcutaneous) treatment was initiated 24 h after stroke and administered once daily for 21 days. Treatment of T2DM-stroke with L-4F improved neurological functional outcome, and decreased hemorrhage, mortality, and BBB leakage identified by decreased albumin infiltration and increased tight-junction and astrocyte end-feet densities, increased cerebral arteriole diameter and smooth muscle cell number, and increased WM density and oligodendrogenesis in the ischemic brain in both ABCA1 and ABCA1 T2DM-stroke mice compared with vehicle-control mice, respectively ( < 0.05, = 9 or 21/group). The L-4F treatment reduced macrophage infiltration and neuroinflammation identified by decreases in ED-1, monocyte chemoattractant protein-1 (MCP-1), and toll-like receptor 4 (TLR4) expression, and increases in anti-inflammatory factor Insulin-like growth factor 1 (IGF-1) and its receptor IGF-1 receptor β (IGF-1Rβ) in the ischemic brain ( < 0.05, = 6/group). These results suggest that post-stroke administration of L-4F may provide a restorative strategy for T2DM-stroke by promoting neurovascular and WM remodeling. Reducing neuroinflammation in the injured brain may contribute at least partially to the restorative effects of L-4F independent of the ABCA1 signaling pathway.
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http://dx.doi.org/10.3389/fneur.2022.863934DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9100936PMC
April 2022

Statistic Copolymers Working as Growth Factor-Binding Mimics of Fibronectin.

Adv Sci (Weinh) 2022 May 15:e2200775. Epub 2022 May 15.

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China.

Growth factors (GFs) play important roles in biological system and are widely used in tissue regeneration. However, their application is greatly hindered by short in vivo lifetime of GFs. GFs are bound to fibronectin dynamically in the extracellular matrix, which inspired the authors to mimic the GF binding domain of fibronectin and design GF-binding amphiphilic copolymers bearing positive charges. The optimal amino acid polymer can bind to a variety of representative GFs, such as bone morphogenetic protein-2 (BMP-2) and TGF-β1 from the transforming growth factor-β superfamily, PDGF-AA and PDGF-BB from the platelet-derived growth factor family, FGF-10 and FGF-21 from the fibroblast growth factor family, epidermal growth factor from the EGF family and hepatocyte growth factor from the plasminogen-related growth factor family, with binding affinities up to the nanomolar level. 3D scaffolds immobilized with the optimal copolymer enable sustained release of loaded BMP-2 without burst release and significantly enhances the in vivo function of BMP-2 for bone formation. This strategy opens new avenues in designing GF-binding copolymers as synthetic mimics of fibronectin for diverse applications.
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http://dx.doi.org/10.1002/advs.202200775DOI Listing
May 2022

Generation of an RNF1-deficient human pluripotent stem cell line using CRISPR/Cas9 technology.

Stem Cell Res 2022 Jul 8;62:102809. Epub 2022 May 8.

CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

RNF1 (RING1A) is a catalytic component of the polycomb repressive complex 1 (PRC1) involved in regulation of, among others, embryonic development and disease progression. However, the exact role of RNF1 in self-renewal and differentiation of human embryonic stem cells (ESCs) remains unknown. Here, we derive one RNF1 knockout human ESC line using CRISPR/Cas9 system. The cell line retains the canonical stem cell morphology and normal karyotype. Moreover, the cell line highly expresses pluripotency genes and has three germ-layer differentiation potential. The RNF1 -/- cell line will be useful for studies on the function and role of RNF1 in human embryonic stem cell fate decisions.
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http://dx.doi.org/10.1016/j.scr.2022.102809DOI Listing
July 2022

Ultrasensitive refractometer based on helical long-period fiber grating near the dispersion turning point.

Opt Lett 2022 May;47(10):2602-2605

Precise and accurate measurements of the optical refractive index (RI) for liquids are increasingly finding applications in biochemistry and biomedicine. Here, we demonstrate a dual-resonance helical long-period fiber grating (HLPFG) near the dispersion turning point (DTP), which exhibits an ultrahigh RI sensitivity (∼25546 nm/RIU at ∼1.440). The achieved RI sensitivity is, to the best of our knowledge, more than one order of magnitude higher than a conventional HLPFG. The ultrahigh RI sensitivity can improve the RI measurement precision and accuracy significantly. Furthermore, ultralow wavelength shifts (nearly zero) with temperature and strain ranging from 20 to 100°C and 0 to 2226 µε, respectively, are also demonstrated for the proposed HLPFG, which may be a good candidate for developing new low-cross-talk sensors.
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http://dx.doi.org/10.1364/OL.456571DOI Listing
May 2022

Application of an infrared thermography-based model to detect pressure injuries: a prospective cohort study.

Br J Dermatol 2022 May 13. Epub 2022 May 13.

College of Nursing, Wenzhou Medical University, Wenzhou, China.

Background: It is challenging to detect pressure injuries at an early stage of their development.

Objectives: To assess the ability of an infrared thermography (IRT)-based model, constructed using a convolution neural network, to reliably detect pressure injuries.

Methods: A prospective cohort study compared validity in patients with pressure injury (n = 58) and without pressure injury (n = 205) using different methods. Each patient was followed up for 10 days.

Results: The optimal cut-off values of the IRT-based model were 0·53 for identifying tissue damage 1 day before visual detection of pressure injury and 0·88 for pressure injury detection on the day visual detection is possible. Kaplan-Meier curves and Cox proportional hazard regression model analysis showed that the risk of pressure injury increased 13-fold 1 day before visual detection with a cut-off value higher than 0·53 [hazard ratio (HR) 13·04, 95% confidence interval (CI) 6·32-26·91; P < 0·001]. The ability of the IRT-based model to detect pressure injuries [area under the receiver operating characteristic curve (AUC) , 0·98, 95% CI 0·95-1·00] was better than that of other methods.

Conclusions: The IRT-based model is a useful and reliable method for clinical dermatologists and nurses to detect pressure injuries. It can objectively and accurately detect pressure injuries 1 day before visual detection and is therefore able to guide prevention earlier than would otherwise be possible. What is already known about this topic? Detection of pressure injuries at an early stage is challenging. Infrared thermography can be used for the physiological and anatomical evaluation of subcutaneous tissue abnormalities. A convolutional neural network is increasingly used in medical imaging analysis. What does this study add? The optimal cut-off values of the IRT-based model were 0·53 for identifying tissue damage 1 day before visual detection of pressure injury and 0·88 for pressure injury detection on the day visual detection is possible. Infrared thermography-based models can be used by clinical dermatologists and nurses to detect pressure injuries at an early stage objectively and accurately.
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http://dx.doi.org/10.1111/bjd.21665DOI Listing
May 2022

Development and Comparison of Multimodal Models for Preoperative Prediction of Outcomes After Endovascular Aneurysm Repair.

Front Cardiovasc Med 2022 26;9:870132. Epub 2022 Apr 26.

Department of Vascular Surgery, Zhongshan Hospital, Institute of Vascular Surgery, Fudan University, National Clinical Research Center for Interventional Medicine, Shanghai, China.

Objective: The aim of this study was to develop and compare multimodal models for predicting outcomes after endovascular abdominal aortic aneurysm repair (EVAR) based on morphological, deep learning (DL), and radiomic features.

Methods: We retrospectively reviewed 979 patients (January 2010-December 2019) with infrarenal abdominal aortic aneurysms (AAAs) who underwent elective EVAR procedures. A total of 486 patients (January 2010-December 2015) were used for morphological feature model development and optimization. Univariable and multivariable analyses were conducted to determine significant morphological features of EVAR-related severe adverse events (SAEs) and to build a morphological feature model based on different machine learning algorithms. Subsequently, to develop the morphological feature model more easily and better compare with other modal models, 340 patients of AAA with intraluminal thrombosis (ILT) were used for automatic segmentation of ILT based on deep convolutional neural networks (DCNNs). Notably, 493 patients (January 2016-December 2019) were used for the development and comparison of multimodal models (optimized morphological feature, DL, and radiomic models). Of note, 80% of patients were classified as the training set and 20% of patients were classified as the test set. The area under the curve (AUC) was used to evaluate the predictive abilities of different modal models.

Results: The mean age of the patients was 69.9 years, the mean follow-up was 54 months, and 307 (31.4%) patients experienced SAEs. Statistical analysis revealed that short neck, angulated neck, conical neck, ILT, ILT percentage ≥51.6%, luminal calcification, double iliac sign, and common iliac artery index ≥1.255 were associated with SAEs. The morphological feature model based on the support vector machine had a better predictive performance with an AUC of 0.76, an accuracy of 0.76, and an F1 score of 0.82. Our DCNN model achieved a mean intersection over union score of more than 90.78% for the segmentation of ILT and AAA aortic lumen. The multimodal model result showed that the radiomic model based on logistics regression had better predictive performance (AUC 0.93, accuracy 0.86, and F1 score 0.91) than the optimized morphological feature model (AUC 0.62, accuracy 0.69, and F1 score 0.81) and the DL model (AUC 0.82, accuracy 0.85, and F1 score 0.89).

Conclusion: The radiomic model has better predictive performance for patient status after EVAR. The morphological feature model and DL model have their own advantages and could also be used to predict outcomes after EVAR.
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http://dx.doi.org/10.3389/fcvm.2022.870132DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9086541PMC
April 2022

Multiple environmental risk assessments of heavy metals and optimization of sludge dewatering: Red mud-reed straw biochar combined with Fe activated HO.

J Environ Manage 2022 Aug 10;316:115210. Epub 2022 May 10.

School of Resource and Environmental Science, Wuhan University, Wuhan, 430072, Hubei, China. Electronic address:

In this study, Fe-rich biochar (RMRS-BC) was prepared from red mud and reed straw to improve sludge dewatering and transformation of heavy metals (HMs, including Cd, Cr, Cu, Pb, and Zn). The optimal concentrations of RMRS-BC, Fe, and HO to promote sludge dewaterability were identified by response surface methodology (RSM). The optimal dosages of RMRS-BC, Fe, and HO were 74.0, 104.9, and 75.7 mg/g dry solids (DS), respectively. The corresponding capillary suction time (CST) and water content of sludge cake were 14.3 s and 51.25 wt%. For the improvement mechanism, heterogeneous and homogeneous Fenton reactions occurred due to RMRS-BC and Fe activating HO. The extracellular polymeric substances (EPS) decomposed into dissolved organic matter (proteins and polysaccharides), thereby promoting the transformation of bound water to free water and further reducing the water content of the sludge cake. The research quantitatively assessed the environmental risk of heavy metals in the conditioned sludge cake based on bioavailability and ecotoxicity, pollution levels and potential ecological risks. Compound conditioning using RMRS-BC, Fe, and HO could significantly improve the solubility and reduce the leaching toxicity of HMs. In general, RMRS-BC combined with Fe to activate HO provided an effective method to enhance sludge dewaterability and reduce HMs risk.
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http://dx.doi.org/10.1016/j.jenvman.2022.115210DOI Listing
August 2022
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