Publications by authors named "Min Zhou"

1,509 Publications

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Visible and near-infrared microdisk resonators on a 4H-silicon-carbide-on-insulator platform.

Opt Lett 2021 Jun;46(12):2952-2955

Wavelength-sized microdisk resonators were fabricated on a single crystalline 4H-silicon-carbide-on-insulator (4H-SiCOI) platform. By carrying out micro-photoluminescence measurements at room temperature, we show that the microdisk resonators support whispering-gallery modes (WGMs) with quality factors up to 5.25×10 and mode volumes down to 2.61×(/) at the visible and near-infrared wavelengths. Moreover, the demonstrated wavelength-sized microdisk resonators exhibit WGMs whose resonant wavelengths are compatible with the zero-phonon lines of silicon related spin defects in 4H-SiCOI, making them a promising candidate for applications in cavity quantum electrodynamics and integrated quantum photonic circuits.
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http://dx.doi.org/10.1364/OL.424540DOI Listing
June 2021

Observation of varicella zoster virus-induced acute retinal necrosis: viral load detection and visual outcome.

Eye (Lond) 2021 Jun 11. Epub 2021 Jun 11.

Department of Ophthalmology, Eye and ENT Hospital of Fudan University, Shanghai, China.

Objectives: To observe the changes of viral load in aqueous humour samples and visual outcomes in varicella zoster virus (VZV)-induced acute retinal necrosis (ARN).

Methods: Observational retrospective study. Medical records and viral load measured by real-time quantitative polymerase chain reaction (qPCR) of 20 eyes with VZV-induced ARN were reviewed.

Results: The mean viral load at presentation was 5.7 × 10 ± 9.7 × 10 copies/mL. An initial plateau phase for viral load lasting up to 2 weeks occurred in most eyes (18 eyes, 90%). In the following logarithmic reduction phase, the mean slope of the decline in viral load was -0.103 ± 0.029 log/day, and the expected time for half reduction of the initial viral load was 3.2 ± 1.0 days. At the end of the first 8-week's antiviral treatment, the viral load was below detection threshold in all 20 eyes (100.0%). The mean logarithm of the minimum angle of resolution (logMAR) best-corrected visual acuity (BCVA) improved from 1.1 ± 0.7 (Snellen equivalent 20/250) to 0.7 ± 0.6 (Snellen equivalent 20/100) after a follow-up of 8.6 ± 2.0 months. Thirteen of the 20 eyes (65.0%) suffered retinal detachment and underwent vitrectomy. The initial viral load was the independent predictive factor of logMAR BCVA at the last follow-up (β = 0.745, P < 0.001).

Conclusions: The observation of viral load changes by qPCR was useful for better monitoring of therapeutic efficacy and deciding needed antiviral duration in VZV-induced ARN patients.
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http://dx.doi.org/10.1038/s41433-021-01609-8DOI Listing
June 2021

A Highly Stable Photodetector Based on a Lead-Free Double Perovskite Operating at Different Temperatures.

J Phys Chem Lett 2021 Jun 11:5682-5688. Epub 2021 Jun 11.

College of Materials Science and Engineering, Key Laboratory of Advanced Materials of Yunnan Province, Kunming University of Science and Technology, Kunming 650093, Yunnan, China.

In recent years, considerable breakthroughs have been achieved in the explored photodetectors with improved performance and stability. However, such devices suffer from the drifting parameters (photoresponsivity, response time, and specific detectivity) in the case of evident operating temperature changes. Here, a double perovskite CsNaBiCl-based ultraviolet (UV) photodetector is developed free from thermal disturbance, exhibiting a steady photoresponsivity (≈ 67.98 mA/W) and response time (≈ 16.42 ms) within a wide temperature range (from 273 to 333 K). Further studies demonstrate that the stability of the crystal structure endows the superior photodetection capability. This result unambiguously highlights the great potential of such double perovskite CsNaBiCl compound as an environmentally friendly alternative for UV photodetectors.
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http://dx.doi.org/10.1021/acs.jpclett.1c01416DOI Listing
June 2021

Association of Gestational Diabetes Mellitus with Adverse Pregnancy Outcomes and Its Interaction with Maternal Age in Chinese Urban Women.

J Diabetes Res 2021 18;2021:5516937. Epub 2021 May 18.

Department of Obstetrics and Gynecology, Peking University First Hospital, Beijing 10034, China.

Background: The prevalence of gestational diabetes mellitus (GDM) has been dramatically increasing worldwide. The aims of this study were to examine associations of GDM with pregnancy outcomes in Chinese urban women and to evaluate the interaction between GDM and other major risk factors for the risk of adverse pregnancy outcomes.

Methods: A retrospective analysis included 8844 women who delivered live singletons at ≥28 weeks of gestation between June 2012 and March 2013 among Chinese urban women. Structured questionnaires were used to collect information on demographic characteristics, lifestyle behavior, medical history, and pregnancy outcomes. The diagnosis of GDM was made between 24 and 28 gestational weeks according to the International Association of Diabetes and Pregnancy Study Groups criteria. Logistic regression models were used to assess the association of GDM with pregnancy outcomes and to examine the interaction between GDM and other major risk factors including maternal age, prepregnancy body mass index, and gestational weight gain for the risk of pregnancy outcomes.

Results: 13.9% of women were diagnosed with GDM. We found that GDM was associated with higher risk of cesarean delivery (odds ratio (OR) = 1.69, 95% CI (confidence interval): 1.48-1.92), preterm birth (OR = 1.32, 95% CI: 1.07-1.64), macrosomia (OR = 1.69, 95% CI: 1.34-2.13), and large for gestational age (LGA, OR = 1.43, 95% CI: 1.18-1.73) after adjustment for potential confounders. We also observed the interaction between GDM and maternal age for the risk of cesarean delivery ( for interaction = 0.025), and the OR of GDM for cesarean delivery was 1.71 (95% CI: 1.49-1.97) among women aged less than 35 years.

Conclusions: GDM was associated with an increased risk of cesarean delivery, preterm birth, macrosomia, and LGA in Chinese urban women, and there was an interaction between GDM and maternal age for the risk of cesarean delivery.
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http://dx.doi.org/10.1155/2021/5516937DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8154302PMC
May 2021

CD47 blockade enhances therapeutic efficacy of cisplatin against lung carcinoma in a murine model.

Exp Cell Res 2021 Jun 7;405(2):112677. Epub 2021 Jun 7.

Department of Anesthesiology and Surgical Intensive Care Unit, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200092, China. Electronic address:

Cisplatin (CDDP) is the first generation of platinum-based drug and is widely used to treat many cancers due to its potency. The present study aims to explore the effects of CDDP on lung carcinoma and its relationship with macrophage phagocytosis. In in vitro study, murine and human lung cancer cell lines were applied and treated with CDDP, CD47 antibody (aCD47), or CDDP plus aCD47. In in vivo study, a tumor xenograft animal model was treated with CDDP, aCD47, or CDDP plus aCD47. Real-time PCR was applied to determine the mRNA expressions. Enzyme-linked immunosorbent assay (ELISA), Western blotting, and Immunofluorescent staining were applied to determine the protein expressions. Flow cytometry was applied to analyze cell apoptosis, phagocytosis, and specific cell populations. CDDP enhanced the expressions of CD47 in lung cancer cells. Interestingly, the blockage of CD47 enhanced the macrophages' phagocytic activity on the CDDP-treated tumor cells. The treatment of CDDP and aCD47 exhibited anti-tumor effects and prolonged the LLC tumor-bearing mice survival time. Mechanistic studies revealed that the treatment of CDDP and aCD47 regulated the phagocytic activity of macrophage, percentage of CD8 T cells, and cytokines (tumor growth factor (TGF)-β, interleukin (IL)12p70, and interferon (IFN)-γ) in the tumor-bearing model. CD47 blockade enhanced therapeutic efficacy of cisplatin against lung carcinoma in vivo and in vitro.
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http://dx.doi.org/10.1016/j.yexcr.2021.112677DOI Listing
June 2021

Corneal Endothelium:A Promising Quantitative Index for Graves Ophthalmopathy Activity Evaluation.

Am J Ophthalmol 2021 Jun 5. Epub 2021 Jun 5.

Department of Ophthalmology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, Guangdong Province, China. Electronic address:

Purpose: To investigate the corneal endothelium damage in Graves ophthalmopathy (GO) and its role as a promising quantitative index to evaluate GO activity.

Design: Cross-sectional study.

Methods: This study included 128 eyes of 64 patients with GO. All subjects underwent ophthalmologic examinations, including proptosis, tear break-up time (BUT), corneal fluorescein staining, Schirmer test. Corneal endothelium was measured by noncontact specular microscope and ocular biometric parameters were measured by IOL Master 700. Each eye was assigned with a specific clinical activity score (CAS), then grouped as Active (CAS ≥ 3 points) or Inactive (CAS<3 points). Ocular parameters between the two groups were compared using generalized estimating equations accounting for intereye correlation, and receiver operating characteristic (ROC) curves were also obtained.

Main Outcome Measures: Parameters of corneal endothelium.

Results: Among the included eyes, 81 eyes had inactive GO and 47 eyes had active GO. Corneal endothelial cell morphology was altered in active GO compared with inactive GO. The coefficient variation of cell area (CV) was significantly higher in active GO compared with inactive GO (37.0(34.4-41.2)% vs. 33.9(30.9-36.8)%, P=0.001), and positively correlated with CAS (r=0.322, P<0.001). Moreover, CV showed a diagnostic capacity to differentiate the active eyes from inactive eyes. The area under the ROC curve (AUC) was 0.705.

Conclusions: Active GO had morphological changes in corneal endothelium compared with inactive GO. CV is a sensitive indicator reflected corneal endothelial function, and has the potential to be adapted as a non-invasive, objective, and quantitative index for evaluating the activity status of GO patients.
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http://dx.doi.org/10.1016/j.ajo.2021.05.020DOI Listing
June 2021

Dissemination of IncFII plasmids carrying fosA3 and bla in clinical isolates of Salmonella enteritidis.

Zoonoses Public Health 2021 Jun 4. Epub 2021 Jun 4.

Department of Food Science & Technology, School of Agriculture and Biology, State Key Lab of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai, China.

Multidrug-resistant Salmonella Enteritidis (S. Enteritidis) isolates have become a significant threat to public health, and fosfomycin has been proposed as one of the therapeutic antibiotics for serious infections by resistant pathogens. In this study, a total of 501 clinical S. Enteritidis isolates were screened and 14 (2.8%) isolates exhibited resistance to fosfomycin (MIC ≥ 1,024 μg/mL) as well as ceftriaxone (MIC ≥ 128 μg/mL). The fosA3 gene was identified in these 14 isolates. The fosA3 gene that co-transferred with bla was observed on the IncFII plasmids with sizes of ~ 78 (n = 7) or ~ 111 (n = 2) kbp in 9 transconjugants. The fosA3-bearing plasmid p12367A is 111,764 bp in length and possessed a typical IncFII backbone. A 7.6-kbp multidrug resistance region (MRR) was identified in p12367A, which was comprised of fosA3 and bla genes interspersed with ΔISEcp1 and three copies of IS26. Two typical antibiotic resistance determinants (IS26-orf3-orf2-orf1-fosA3-IS26 and IS26-orf477-bla -ΔISEcp1-IS26) shared one IS26 in the MRR. The genetic arrangement of the MRR may have resulted from the stepwise integration of IS26 mobile elements via homologous recombination. Horizontal transfer of IncFII plasmids might contribute to the dissemination of fosA3 and bla resistance genes in S. Enteritidis interspecies. These findings underline further challenges for the prevention and treatment of Enterobacteriaceae infections posed by epidemic IncFII plasmids bearing fosA3-bla .
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http://dx.doi.org/10.1111/zph.12825DOI Listing
June 2021

Role and mechanism underlying FoxO6 in skeletal muscle and .

Int J Mol Med 2021 Jul 3;48(1). Epub 2021 Jun 3.

Department of General Surgery, Chengdu Fifth People's Hospital, The Fifth People's Hospital Affiliated with Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan 611130, P.R. China.

Skeletal muscle atrophy is a common feature of patients suffering with chronic infection and other systemic diseases, including acquired immunodeficiency syndrome, chronic kidney disease and cancer. Therefore, understanding the molecular basis of muscle loss is of importance. The majority of members of the forkhead box O (FoxO) family can induce skeletal muscle atrophy; however, the effect of FoxO6 on skeletal muscle is not completely understood. The present study investigated the role of FoxO6 and . Compared with the small interfering RNA (si)‑negative control (NC) group, CC cell proliferation (Cell Counting Kit‑8 assay), myotube differentiation and myotube production were significantly decreased by FoxO6 knockdown, which was different from the known functions of other FoxO members. The immunofluorescence assay results demonstrated that si‑FoxO6 clearly downregulated the expression levels of myosin heavy chain (MyHC) in CC myotubes compared with si‑NC. The western blotting results indicated that compared with the si‑NC group, FoxO6 knockdown induced CC myotube atrophy by notably downregulating myoblast determination protein 1 (MyoD), mTOR and MyHC expression levels, and by markedly upregulating ubiquitin ligase (atrogin1) and muscle RING‑finger protein‑1 (MURF1) expression levels. Similarly, in an model of TNF‑α‑induced myotube atrophy, the western blotting results indicated that FoxO6 expression levels were decreased, whereas atrogin1, MURF1, FoxO1 and FoxO3a expression levels were increased compared with the control group. Therefore, the results indicated that, unlike FoxO1 or FoxO3a, FoxO6 maintained CC myotubes and protected against atrophy. Consistent with the data, similar results were observed . Collectively, the results of the present study suggested that FoxO6 served a critical role in muscle cell metabolism and , and might serve as a promising therapeutic target for ameliorating skeletal muscle atrophy.
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http://dx.doi.org/10.3892/ijmm.2021.4976DOI Listing
July 2021

Arsenic Combined With All-Trans Retinoic Acid for Pediatric Acute Promyelocytic Leukemia: Report From the CCLG-APL2016 Protocol Study.

J Clin Oncol 2021 Jun 2:JCO2003096. Epub 2021 Jun 2.

Anhui Provincial Children's Hospital, Hefei, China.

Purpose: Arsenic combined with all-trans retinoic acid (ATRA) is the standard of care for adult acute promyelocytic leukemia (APL). However, the safety and effectiveness of this treatment in pediatric patients with APL have not been reported on the basis of larger sample sizes.

Methods: We conducted a multicenter trial at 38 hospitals in China. Patients with newly diagnosed APL were stratified into two risk groups according to baseline WBC count and mutation. ATRA plus arsenic trioxide or oral arsenic without chemotherapy were administered to the standard-risk group, whereas ATRA, arsenic trioxide, or oral arsenic plus reduced-dose anthracycline were administered to the high-risk group. Primary end points were event-free survival and overall survival at 2 years.

Results: We enrolled 193 patients with APL. After a median follow-up of 28.9 months, the 2-year overall survival rate was 99% (95% CI, 97 to 100) in the standard-risk group and 95% (95% CI, 90 to 100) in the high-risk group ( = .088). The 2-year event-free survival was 97% (95% CI, 93 to 100) in the standard-risk group and 90% (95% CI, 83 to 96) in the high-risk group ( = .252). The plasma levels of arsenic were significantly elevated after treatment, with a stable effective level ranging from 42.9 to 63.2 ng/mL during treatment. In addition, plasma, urine, hair, and nail arsenic levels rapidly decreased to normal 6 months after the end of treatment.

Conclusion: Arsenic combined with ATRA is effective and safe in pediatric patients with APL, although long-term follow-up is still needed.
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http://dx.doi.org/10.1200/JCO.20.03096DOI Listing
June 2021

Cytokine levels and pathological characteristics of a patient with severe coronavirus disease 2019: a case report.

Chin Med J (Engl) 2021 Jun 2. Epub 2021 Jun 2.

Department of Critical Care Medicine, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, China Institute of Critical Care Medicine, The Sino Russian Medical Research Center of Harbin Medical University, Harbin, Heilongjiang 150081, China Key Laboratory of Hepatosplenic Surgery, Ministry of Education, Harbin, Heilongjiang 150001, China The Cell Transplantation Key Laboratory of National Health Commission, Harbin, Heilongjiang 150001, China Department of Critical Care Medicine, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, China Department of Critical Care Medicine, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, China Department of Critical Care Medicine, Harbin Medical University Cancer Hospital, Harbin, Heilongjiang 150081, China Department of Pathology, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, China Department of Critical Care Medicine, Shuang Kuang Hospital, Shuang Ya-shan, Heilongjiang 155100, China.

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http://dx.doi.org/10.1097/CM9.0000000000001540DOI Listing
June 2021

EHR-oriented Knowledge Graph System: Toward Efficient Utilization of Non-used Information Buried in Routine Clinical Practice.

IEEE J Biomed Health Inform 2021 May 31;PP. Epub 2021 May 31.

Non-used clinical information has negative implications on healthcare quality. Clinicians pay priority attention to clinical information relevant to their specialties during routine clinical practices but may be insensitive or less concerned about information showing disease risks beyond their professions, resulting in delayed, missed diagnoses or improper management. In this study, we introduced an EHR-oriented knowledge graph system to efficiently utilize non-used information buried in electronic health records (EHRs). EHR data were transformed into a semantic patient-centralized information model under the ontology structure of a knowledge graph. The knowledge graph then creates an EHR data trajectory and performs reasoning through semantic rules to identify important clinical findings within EHR data. A graphical reasoning pathway illustrates the reasoning footage and explains the clinical significance for clinicians to better understand the neglected information. An application study was performed to evaluate the unconsidered chronic kidney disease (CKD) reminding for non-nephrology clinicians to identify important neglected information. The study covered 71,679 patients in non-nephrology departments. The system identified 2,774 patients meeting CKD diagnosis criteria and 10,377 patients requiring high attention. A follow-up study of 5,439 patients showed 80.7% of patients meeting the diagnosis criteria and 61.4% of patients requiring high attention were confirmed to be CKD positive during follow-up research. The application demonstrated that the purposed approach is feasible and effective in clinical information utilization. Additionally, it's valuable as an explainable artificial intelligence to provide interpretable recommendations for specialist physicians to understand the importance of non-used data and make comprehensive decisions.
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http://dx.doi.org/10.1109/JBHI.2021.3085003DOI Listing
May 2021

Do inflammaging and coagul-aging play a role as conditions contributing to the co-occurrence of the severe hyper-inflammatory state and deadly coagulopathy during COVID-19 in older people?

Exp Gerontol 2021 May 26;151:111423. Epub 2021 May 26.

Department of Geriatrics, Shanghai Institute of Geriatrics, Huadong Hospital, Fudan University, Shanghai, PR China. Electronic address:

The coronavirus disease 2019 (COVID-19) is a new infectious respiratory disease, which has caused a pandemic that has become the world's leading public health emergency, threatening people of all ages worldwide, especially the elderly. Complications of COVID-19 are closely related to an upregulation of the inflammatory response revealed by the pro-inflammatory profile of plasma cytokines (to the point of causing a cytokine storm), which is also a contributing cause of the associated coagulation disorders with venous and arterial thromboembolisms, causing multiple organ dysfunction and failure. In severe fulminant cases of COVID-19, there is an activation of coagulation and consumption of clotting factors leading to a deadly disseminated intravascular coagulation (DIC). It is well established that human immune response changes with age, and also that the pro-inflammatory profile of plasma cytokines is upregulated in both healthy and diseased elderly people. In fact, normal aging is known to be associated with a subclinical, sterile, low-grade, systemic pro-inflammatory state linked to the chronic activation of the innate immune system, a phenomenon known as "inflammaging". Inflammaging may play a role as a condition contributing to the co-occurrence of the severe hyper-inflammatory state (cytokine storm) during COVID-19, and also in other severe infections (sepsis) in older people. Moreover, we must consider the impact of inflammation on coagulation due to the crosstalk between inflammation and coagulation. The systemic inflammatory state and coagulation disorders are closely related, a phenomenon that here we call "coagul-aging" (Giunta S.). In this review, we discuss the various degrees of inflammation in older adults after being infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), and the adverse effects of aging on the inflammatory response and coagulation system. It is important to note that although there is no gender difference in susceptibility to COVID-19 infection, however, due to differences in angiotensin-converting enzyme 2 (ACE2) expression, innate immunity, and comorbidities, older men exhibit more severe disease and higher mortality than older women. There are currently no FDA-approved specific antiviral drugs that can be used against the virus. Therapies used in patients with COVID-19 consist of remdesivir, dexamethasone, low-molecular-weight heparin, in addition to monoclonal antibodies against the spike protein of SARS-CoV-2 in the early phase of the disease. Future pharmacological research should also consider targeting the possible role of the underlying scenario of inflammaging in healthy older people to prevent or mitigate disease complications. It is worth mentioning that some specific cytokine antagonists and traditional Chinese medicine preparations can reduce the elderly's inflammatory state.
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http://dx.doi.org/10.1016/j.exger.2021.111423DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8149167PMC
May 2021

A new insight to characterize immunomodulation based on hepatopancreatic transcriptome and humoral immune factor analysis of the Cherax quadricarinatus infected with Aeromonas veronii.

Ecotoxicol Environ Saf 2021 Aug 25;219:112347. Epub 2021 May 25.

Aquaculture Research Lab, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian 271018, China. Electronic address:

Cherax quadricarinatus is a type of large freshwater crayfish that is characterized by rapid growth and formidable adaptability. It has also been widely cultured and studied as a model organism. Aeromonas veronii is the dominant pathogen in aquatic environments and the primary threat to aquaculture's economic stability. To better understand the interactions between C. quadricarinatus and A. veronii, high-throughput RNA sequencing of the C. quadricarinatus hepatopancreas was carried out on a control group, susceptible group (6 h after infection), and resistant group (48 h after infection). A total of 65,850,929 genes were obtained. Compared with the control group, 2616 genes were up-regulated and 1551 genes were down-regulated in the susceptible group; while 1488 genes were up-regulated and 1712 genes were down-regulated in the resistant group. GO and KEGG analysis showed that these differentially expressed genes (DEGs) were associated with multiple immune pathways, including Toll-like receptors (TLRs), antigen processing and presentation, NOD-like receptor signaling pathway, phagosome, lysosome, JAK-STAT signaling pathway. qRT-PCR showed that infection by A. veronii changed the expression pattern of the serine proteinase inhibitor (SPI), crustacean hyperglycemic hormone (CHH), anti-lipopolysaccharide factor (ALF), and extracellular copper/zinc superoxide dismutase (SOD1), all of which were significantly higher than in the control group up to 48 h after infection. In addition, detection of superoxide dismutase (SOD), catalase (CAT), lysozyme (LZM), and phenoloxidase (PO) activity, as well as ceruloplasmin (CP) concentration at different times after infection showed diverse trends. Furthermore, pathological sections obtained 24 h after infection show lesions on the hepatopancreas and intestinal tissues caused by A. veronii. The results of this study provide a foundation for analyzing the immune mechanism of C. quadricarinatus infected with A. veronii at the transcriptional level and a theoretical basis for screening disease-resistant individuals to ensure healthy economic development of the aquatic industry.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112347DOI Listing
August 2021

Risk factors for ophthalmic artery stenosis and occlusion in patients with retinoblastoma treated with intra-arterial chemotherapy.

Br J Ophthalmol 2021 May 26. Epub 2021 May 26.

Department of Ophthalmology, Shanghai Key Laboratory of Orbital Diseases and Ocular Oncology, Shanghai Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, P. R. China

Purpose: To explore the risk factors for ophthalmic artery (OA) stenosis and occlusion after intra-arterial chemotherapy (IAC) with selective ophthalmic artery catheterisation (OAC) in the treatment of retinoblastoma.

Design: Retrospective, single centre case-control study.

Methods: The study was conducted including consecutive patients with unilateral or bilateral intraocular retinoblastoma undergoing IAC between June 2016 and June 2019 with a follow-up time of 4 years. Main outcomes are rate of IAC-induced OA occlusion and OA diameter.

Results: 346 attempted OAC infusions were successful. The total incidence of OA occlusion was 15.89%. The occlusion and control groups were similar in patients' age, sex and disease stage. Median OA diameter was 0.49 mm in those with OA occlusion, and 0.66 mm in those without occlusion. In the occlusion group, the OA diameter difference was significantly larger between the first IAC and the final IAC (0.22mm vs 0.12mm, p=0.001). In both groups, the median number of IAC treatments was 3. Multivariate Cox regression models included initial OA diameter (OR: 0.005, p=0.001), ratio of OA orifice diameter differences between first and last IAC to the initial OA orifice diameter (OR: 4.661, p=0.003), and number of IAC (OR: 1.538, p=0.042) as clinical features significantly associated with OA occlusion.

Conclusions: The OA diameter at first IAC treatment, the ratio of OA orifice diameter differences between first and last IAC to the initial OA orifice diameter and total number of IAC treatments may be three main clinical predictors for OA occlusion after IAC for retinoblastoma.
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http://dx.doi.org/10.1136/bjophthalmol-2021-319118DOI Listing
May 2021

Low-dose X-ray enhanced tumor accumulation of theranostic nanoparticles for high-performance bimodal imaging-guided photothermal therapy.

J Nanobiotechnology 2021 May 26;19(1):155. Epub 2021 May 26.

Eye Center, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310009, China.

Background: Theranostic nanoparticles (NPs) have achieved rapid development owing to their capacity for personalized multimodal diagnostic imaging and antitumor therapy. However, the efficient delivery and bulk accumulation of NPs in tumors are still the decisive factors in improving therapeutic effect. It is urgent to seek other methods to alters tumor microenvironment (like vascular permeability and density) for enhancing the efficiency of nanoparticles delivery and accumulation at the tumor site.

Methods: Herein, we developed a Raman-tagged hollow gold nanoparticle (termed as [email protected]) with surface-enhanced Raman scattering (SERS) property, which could be accumulated efficiently in tumor site with the pre-irradiation of low-dose (3 Gy) X-ray and then exerted highly antitumor effect in breast cancer model.

Results: The tumor growth inhibition (TGI) of [email protected] photothermal therapy (PTT) was increased from 60% for PTT only to 97%, and the lethal distant metastasis of 4T1 breast cancer (such as lung and liver) were effectively inhibited under the X-ray-assisted PTT treatment. Moreover, with the strong absorbance induced by localized surface plasmon resonance in near-infrared (NIR) region, the signals of Raman/photoacoustic (PA) imaging in tumor was also significantly enhanced after the administration of [email protected], indicating it could be used as the Raman/PA imaging and photothermal agent simultaneously under 808 nm laser irradiation.

Conclusions: Our studied of the as-prepared [email protected] integrated the Raman/PA imaging and PTT functions into the single platform, and showed the good prospects for clinical applications especially with the low-dose X-ray irradiation as an adjuvant, which will be a productive strategy for enhancing drug delivery and accumulation in tumor theranostics.
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http://dx.doi.org/10.1186/s12951-021-00875-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8152352PMC
May 2021

Platelet Membrane-Coated and VAR2CSA Malaria Protein-Functionalized Nanoparticles for Targeted Treatment of Primary and Metastatic Cancer.

ACS Appl Mater Interfaces 2021 Jun 26;13(22):25635-25648. Epub 2021 May 26.

Department of Pharmacy, The Second Affiliated Hospital of Army Medical University, ChongQing 400037, China.

Metastasis is the main cause of death in cancer patients. The efficacy of pharmacological therapy for cancer is limited by the heterogeneous nature of cancer cells and the lack of knowledge of microenvironments in metastasis. Evidence has shown that activated platelets possess both tumor-homing and metastasis-targeting properties via intrinsic cell adhesion molecules on platelets, and malaria protein VAR2CSA is able to specifically bind to oncofetal chondroitin sulfate, which is overexpressed on cancer cells with both epithelial and mesenchymal phenotypes. Inspired by these mechanisms, we developed a recombinant VAR2CSA peptide (rVAR2)-modified activated platelet-mimicking nanoparticles (rVAR2-PM/PLGA-ss-HA) by coating the surface of disulfide-containing biodegradable PLGA conjugate nanoparticles (PLGA-ss-HA) with an activated platelet membrane. The results demonstrated that the engineered 122 nm rVAR2-PM/PLGA-ss-HA inherited the innate properties of the activated platelet membrane and achieved enhanced homing to both primary and metastatic foci. The nanoparticles were endocytosed and responded to a high intracellular concentration of reduced glutathione, resulting in nanoparticle disintegration and the release of chemotherapeutic drugs to kill tumor cells. Thus, rVAR2-decorated activated platelet-targeting nanoparticles with controlled drug release provide a promising drug delivery strategy for efficient treatment of primary and metastatic cancer.
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http://dx.doi.org/10.1021/acsami.1c02581DOI Listing
June 2021

Acrylamide exposure and pulmonary function reduction in general population: The mediating effect of systemic inflammation.

Sci Total Environ 2021 Jul 9;778:146304. Epub 2021 Mar 9.

Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China; Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China. Electronic address:

Acrylamide exposure along with resultant potential adverse health effects have attracted global concern, and the World Health Organization calls for more and urgent studies on the health risks from acrylamide. However, the association and mechanism between acrylamide exposure and pulmonary dysfunction remain unclear. Our goals were to investigate the relationship of internal acrylamide exposure with lung function reduction, and the potential mediating role of systematic inflammation in that relationship. Our study was conducted within the Wuhan-Zhuhai cohort. Urinary biomarkers of acrylamide exposure (N-acetyl-S-(2-carbamoylethyl)-l-cysteine, AAMA; N-acetyl-S-(2-carbamoyl-2-hydroxyethyl)-l-cysteine, GAMA) and lung function were determined among 3271 general adults, of whom 2595 had test results of systemic inflammatory marker plasma C-reactive protein (CRP). We employed linear mixed models to assess the relations among urinary acrylamide metabolites, pulmonary function and plasma CRP, and PRODCLIN program to evaluate the mediating role of CRP. We observed that urinary acrylamide metabolites were inversely and dose-dependently related to lung function (P trend<0.05). Each 1-unit increment in log-transformed level of AAMA, GAMA, or AAMA+GAMA (ΣUAAM) was significantly (P < 0.05) related to a 59.9-, 64.2-, or 64.3-mL reduction in FVC, and a 53.9-, 59.7-, or 58.5-mL reduction in FEV, respectively. Such relationships were independent of smoking, and were significant in physically inactive rather than physically active participants. AAMA (β = 0.10), GAMA (β = 0.16) and ΣUAAM (β = 0.12) were significantly (P < 0.05) related to increased CRP, which was significantly (P < 0.05) related to reduced FVC (β = -55.3) and FEV (β = -40.6). We further found that increased CRP significantly (P < 0.05) mediated 6.34-11.1% of the urinary acrylamide metabolites-associated lung function reductions. For the first time, our findings suggested that exposure to acrylamide in daily life was related to reduced lung function and increased systemic inflammation in general population, and systemic inflammation further mediated acrylamide-associated lung function reduction, indicating a potential mechanistic role of systemic inflammation underlying pulmonary dysfunction from acrylamide exposure.
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http://dx.doi.org/10.1016/j.scitotenv.2021.146304DOI Listing
July 2021

Effects of dissolved oxygen on the sludge dewaterability and extracellular polymeric substances distribution by bioleaching.

Chemosphere 2021 May 17;281:130906. Epub 2021 May 17.

School of Environment, Henan Normal University, China.

Bioleaching is a biological conditioning technology for sludge, which not only improves sludge dewatering performance but also removes heavy metals from sludge. As the bioleaching process is a comprehensive biological and chemical process, it is necessary to explore the effects of dissolved oxygen (DO) concentrations on bioleaching efficiency. Three bioleaching experiments with different DO concentrations (T1: 0.8-3.1 mg/L, T2: 3.1-5.5 mg/L, T3: 5.5-7.5 mg/L) were conducted for five days. The sludge dewatering efficiency was evaluated using capillary suction time (CST) and specific resistance to filtration (SRF). The relationship between sludge dewaterability and extracellular polymeric substance (EPS) fraction distribution was investigated. In the treatment with the highest DO concentration, the minimum values of SRF and CST were 4.31 × 10 m/kg and 13.5 s, which occurred earlier than the treatment with the lower DO concentrations by approximately 24-48 h. A significant decrease (83.4-93.2%) in tightly bound EPS (TB-EPS) protein (PN) was observed in all treatments, while a positive correlation (r = 0.924, P < 0.01) was observed between SRF and PN content in TB-EPS. A relatively higher abundance of Acidithiobacillus was found with the increase in DO concentration. Additionally, other genera including Metallibacterium, Alicyclobacillus, Acidibacter, Acidocella, and Luteococcus also played important roles in EPS biodegradation. These results revealed that increasing the DO concentration could improve sludge dewatering performance and heavy metal removal by enhancing bioleaching microbial activity, the degradation of PN in TB-EPS, and sludge floc fragmentation, but only if sufficient energy sources were provided.
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http://dx.doi.org/10.1016/j.chemosphere.2021.130906DOI Listing
May 2021

Impact of propofol epidural anesthesia on immune function and inflammatory factors in patients undergoing gastric cancer surgery.

Am J Transl Res 2021 15;13(4):3064-3073. Epub 2021 Apr 15.

Department of Anesthesia and Operation, Yunnan Cancer Hospital, The Third Affiliated Hospital of Kunming Medical University, Yunnan Cancer Center Kunming, Yunnan Province, China.

Objective: To investigate the impact of propofol epidural anesthesia on indexes such as T lymphocytes, NK cells and inflammatory factors in patients undergoing gastric cancer surgery.

Methods: Eighty patients undergoing laparoscopic radical gastrectomy were randomly divided into the control group and the observation group, with 40 cases in each group. The control group was given propofol intravenous anesthesia, while the observation group was given propofol epidural anesthesia. The anesthetic indexes, mean arterial pressure (MAP), bispectral index (BIS), level of serum inflammatory mediators, level of T lymphocytes, level of NK cells and safety analysis were observed during anesthesia.

Results: The anesthesia onset time, complete block time, time to resume spontaneous breathing and orientation recovery time in the observation group were reduced, and there was a difference compared with the control group (P<0.05). MAP before anesthesia (T0), after tracheal intubation (T1), at 30 min during operation (T2), at 60 min during operation (T3) and at the end of the operation (T4) all had a relatively small overall fluctuation. MAP at T2 and T3 in the observation group was lower than that in the control group (P<0.05). BIS value at T2 and T3 in the observation group was lower than that in the control group (P<0.05). Compared with the control group, the expression of IL-6 and TNF-α in the observation group decreased after tracheal intubation (S1), 1 d after the operation (S2), 3 d after the operation (S3), 5 min after extubation (S4) (P<0.05), while there was no significant difference at other time points (P>0.05). For pairwise comparison within each group, IL-1β, IL-6 and TNF-α level at S1 and S2 were upregulated compared with those at S0, and IL-1β, IL-6 and TNF-α level at S3 and S4 were downregulated compared with those at S2 (P<0.05). CD3+ T cells levels at S1, S2, S3 showed a downward trend compared to S0 (P<0.05). Compared with the control group, CD4+ T cells level at S4 increased (P<0.05). CD4+ T cells levels at S3 in the two groups both increased compared with those at S1 and S2, and CD4+ T cells levels at S4 in the two groups both increased compared with those at S1, S2 and S3 (P<0.05). Compared with the control group, CD8+ T cells levels at S3 and S4 in the observation group decreased. CD8+ T cells levels at S1, S2, S3 showed a downward trend compared to S0 (P<0.05), while those at S4 showed an upward trend compared to S1 (P<0.05). Compared with the control group, the CD4+/CD8+ at S4 in the observation group increased (P<0.05). Compared with the control group, NK cells levels at S1-S4 increased (P<0.05), and NK cells levels at S1, S2, S3 showed a downward trend compared to S0 (P<0.05). The incidence of adverse reactions in the observation group was lower than that in the control group (P<0.05).

Conclusion: The anesthetic effect of propofol epidural anesthesia was better than that of intravenous anesthesia in patients undergoing gastric cancer surgery. The main performance was that the expression of inflammatory mediators such as IL-1β, IL-6, TNF-α decreased at different time points before and after anesthesia, the proportion of CD4+ T cells, CD4+/CD8+ and NK cells increased, and the adverse reactions were less, which makes propofol epidural anesthesia worthy of clinical promotion.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8129227PMC
April 2021

Role of bronchoalveolar lavage fluid and serum interleukin-27 in the diagnosis of smear-negative pulmonary tuberculosis.

Medicine (Baltimore) 2021 May;100(20):e25821

Department of Respiratory and Critical Care Medicine.

Background: To evaluate the value of interleukin (IL)-27 measured in serum and bronchoalveolar lavage fluid (BALF) for the diagnosis of smear-negative pulmonary tuberculosis (TB).

Methods: This was a prospective study of patients planned to undergo bronchoscopy at Wuxi No.5 People's Hospital between January 2017 and September 2018. The patients were grouped as the TB and control groups. BALF and serum IL-27 were measured by ELISA. Receiver operating characteristic (ROC) curves were used to assess the diagnostic value and calculate the optimal cutoff values.

Results: There were 40 patients in the control group and 87 in the TB group. In the TB group, 20 had positive sputum smear results and 67 were negative. The area under the ROC curve (AUC) of BALF IL-27 for pulmonary TB was 0.897 (95% CI: 0.830-0.944) (P < .001). The AUC of serum IL-27 for pulmonary TB was 0.703 (95% CI: 0.616-0.781) (P < .001). In patients with negative sputum smear results, the AUCs of BALF IL-27 and serum IL-27 for pulmonary TB was 0.882 (95% confidence interval [CI]: 0.805-0.936) (P < .001) and 0.679 (95% CI: 0.601-0.782) (P < .001), respectively.

Conclusions: BALF IL-27 can be used for the diagnosis of pulmonary TB, particularly in those with a negative sputum smear result. Serum IL-27 could be an auxiliary method for TB screening.
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http://dx.doi.org/10.1097/MD.0000000000025821DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8137093PMC
May 2021

Hedgehog signaling modulates cigarette-induced COPD development.

Exp Ther Med 2021 Jul 4;22(1):729. Epub 2021 May 4.

Department of Respiratory and Critical Care Medicine, Shanghai Rui Jin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200025, P.R. China.

Hedgehog (Hh) signaling is involved in early embryogenesis and maintains quiescence in the adult lungs. The interruption of Hh signaling may lead to the development of chronic obstructive pulmonary disease (COPD). The current study aimed to assess whether the Hh pathway affects cigarette-induced emphysema and airway inflammation by regulating inflammatory cytokines. C57BL/6J mice were randomized into control, cigarette smoke (CS) or CS + cyclopamine (CSC) groups. Control mice were exposed to normal room air, CS mice were exposed to tobacco smoke and CSC mice were exposed to CS and received cyclopamine treatment. Histopathological examination of lung tissues was performed, and the expression of sonic hedgehog (HH), glioma-associated oncogene homolog 1 (Gli1), hedgehog-interacting protein (HIP) and several inflammatory mediators (intracellular adhesion molecule-1, IL-6, IL-8 and TNF-α) were compared using reverse transcription-quantitative PCR and western blotting. The emphysema of lung tissues by histopathological examination demonstrated partial amelioration in the CSC group compared with that in the CS group. Additionally, expression levels of SHH, Gli1 and inflammatory mediators were significantly higher in the CS group compared with the control group but were significantly decreased in the CSC group. The expression of HIP was decreased in the CS group, but significantly increased in the CSC group. Hh signaling may serve an important role in cigarette-induced emphysema and airway inflammation by regulating inflammatory cytokines in animal models. Therefore, diminishing the activation of the Hh signal may serve as a novel therapeutic strategy for patients suffering from smoking-related COPD.
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http://dx.doi.org/10.3892/etm.2021.10161DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8120645PMC
July 2021

MiR-146b-3p regulates proliferation of pancreatic cancer cells with stem cell-like properties by targeting MAP3K10.

J Cancer 2021 3;12(12):3726-3740. Epub 2021 May 3.

Department of Biliary-Pancreatic Surgery, Affiliated Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.

Cancer stem cells (CSCs) initiate and maintain tumorigenesis due to their unique pluripotency. However, pancreatic stem cell gene signatures are not completely revealed yet. Here, we isolated pancreatic cancer stem cells (P-CSCs) and exploited their distinct genome-wide mRNA and miRNA expression profiles using microarrays. CD24 CD44 ESA cells were isolated from two pancreatic xenograft cells by the flow cytometry and identified the stem cell-like properties by the tumor formation, self-renew and chemoresistance. Microarrays and qRT-PCR were used to exploit their distinct Genome-wide mRNA and miRNA expression profiles. The function and candidate target genes of key microRNA were detected after Ectopic restoration in the pancreatic cancer cell lines MIA Paca-2 (CSC) and BxPC-3 (CSC). In this study, we isolated P-CSCs from two xenografts cells. Genome-wide profiling experiments showed 479 genes and 15 microRNAs specifically expressed in the P-CSCs, including genes involved in TGF-β and p53 signaling pathways and particularly miR-146b-3p as the most significantly downregulated miRNA. We confirmed miR-146b-3p as a downregulated signature in pancreatic cancer tissues and cell line MIA Paca-2 (CSC) cells. Ectopic restoration of miR-146b-3p expression with pre-miR reduced cell proliferation, induced apoptosis, increased G1 phase and reduced S phase in cell cycle in MIA Paca-2 (CSC), but not in BxPC-3 (CSC). Re-expression of miR-146b-3p with lentivirus significantly inhibited tumorigenicity in MIA Paca-2, but slightly in BxPC-3. Furthermore, we demonstrated that miR-146b-3p directly targeted MAP3K10 and might activate Hedgehog pathway as well through DYRK2 and GLI2. These results suggest that P-CSCs have distinct gene expression profiles. MiR-146b-3p inhibits proliferation and induced apoptosis in P-CSCs high cells lines by targeting MAP3K10. Targeting P-CSCs specific genes may provide novel strategies for therapeutic purposes.
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http://dx.doi.org/10.7150/jca.48418DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8120187PMC
May 2021

Broadband tunable orbital angular momentum mode converter based on a conventional single-mode all-fiber configuration.

Opt Express 2021 May;29(10):15595-15603

A broadband tunable orbital angular momentum (OAM) mode converter based on a helical long-period fiber grating (HLPFG) inscribed in a conventional single-mode fiber (SMF) is experimentally demonstrated. The proposed all-fiber OAM mode converter is based on the core-cladding mode dual resonance near the dispersion turning point (DTP). The converter can operate with a bandwidth of 303.9 nm @ -3 dB and 182.2 nm @ -10 dB, which is, as far as we know, the widest bandwidth for a conventional SMF. Furthermore, the bandwidth of the OAM mode can be dynamically tuned within a large dynamic range (>80 nm) by simply twisting the fiber clockwise (CW) or counterclockwise (CCW). The dynamic tunability of the bandwidth of the proposed OAM mode generator may find vital applications in large-capacity optical fiber communication systems.
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http://dx.doi.org/10.1364/OE.421967DOI Listing
May 2021

Absolute Neutrophil Count in the Peripheral Blood Predicts Prognosis in Lung Cancer Patients Treated with Anlotinib.

Cancer Manag Res 2021 3;13:3619-3627. Epub 2021 May 3.

Department of Respiratory and Critical Care Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.

Purpose: Anlotinib is a multi-targeted tyrosine kinase inhibitor that inhibits tumor angiogenesis and cell proliferation. It is widely used as a third-line therapy for lung cancer. However, reliable prognostic biomarkers for predicting the efficacy of anlotinib are lacking. We conducted a retrospective study to investigate the prognostic value of serological inflammatory biomarkers in anlotinib treatment.

Patients And Methods: Patients with advanced lung cancer treated with anlotinib monotherapy were enrolled. Cox regression was conducted to analyze the significant factors related to progression-free survival (PFS) and overall survival (OS). The objective response rate (ORR) was compared based on the median cut-off value of the significant inflammation index. Meanwhile, we created survival curves to compare the two groups and performed receiver operating characteristic curve analysis to assess the predictive ability of the inflammation index.

Results: Among a total of 71 patients, the median PFS was 5.5 months and the median OS was 9.5 months. The ORR and disease control rate were 16.9% and 84.5%, respectively. According to univariate and multivariate analyses, absolute neutrophil count (ANC) was the only indicator associated with both PFS (hazard ratio [HR] =1.095, 95% confidence interval [CI] 1.030-1.163, P=0.003) and OS (HR=1.057, 95% CI 1.003-1.113, P=0.037). In the group with ANC ≥4.58, the ORR was relatively lower (8.1% vs 26.5%, P=0.057), but not statistically significant; PFS and OS were relatively shorter (median PFS 5.0 [95% CI 4.4-9.6] vs 7.0 months [95% CI 4.4-5.7], P=0.024 and median OS 7.3 [95% CI 4.7-10.0] vs 17.6 months [95% CI 12.3-22.9], P < 0.001). ANC had a relatively high discriminatory ability to predict 10-month survival, with an area under the curve of 0.729, sensitivity of 82.5%, and specificity of 67.7%.

Conclusion: Elevated pre-treatment ANC was associated with a poor prognosis. Patients with lower peripheral blood levels of ANC might benefit from anlotinib.
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http://dx.doi.org/10.2147/CMAR.S307368DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8106457PMC
May 2021

Identification and Characterization of a Novel SXT/R391 Integrative and Conjugative Element in a Food Isolate.

Foodborne Pathog Dis 2021 May 7. Epub 2021 May 7.

College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, China.

is an opportunistic human pathogen. In this study, a novel SXT/R391 integrative and conjugative element (ICE), named ICEChnS012, was identified in the multidrug-resistant strain S012 that was isolated from retail chicken in China. Whole genome sequencing revealed that ICEPChnS012 carried 22 resistance genes including , , , , and . ICEPChnS012 harbored 10 copies of IS and IS-mediated genetic new rearrangements caused variations in HS4 region. To our knowledge, an unusual gene cassette array was found in in this study for the first time. And this is the first report of identification of and in VRIII region in The conjugation experiments proved that ICEChnS012 could be transferred to EC600 through conjugation. These findings demonstrated that ICEChnS012 was a special ICE that carried the largest number of antimicrobial resistance genes in the family of SXT/R391 ICEs. This element could serve as an important vehicle for the dissemination of antibiotic resistance genes and should receive great concern.
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http://dx.doi.org/10.1089/fpd.2020.2886DOI Listing
May 2021

Intrapulmonary distal airway stem cell transplantation repairs lung injury in chronic obstructive pulmonary disease.

Cell Prolif 2021 Jun 7;54(6):e13046. Epub 2021 May 7.

East Hospital, School of Medicine, Tongji University, Shanghai, China.

Objectives: Chronic obstructive pulmonary disease (COPD) is characterized by irreversible lung tissue damage including chronic bronchitis and emphysema, which could further develop into respiratory failure. Many studies have revealed a potential regenerative function of the distal airway stem/progenitor cells (DASCs) after lung injury.

Materials And Methods: Mouse and human DASCs were expanded, analysed, and engrafted into injured mouse lungs. Single-cell analyses were performed to reveal the differentiation path of the engrafted cells. Finally, human DASCs were transplanted into COPD mice induced by porcine pancreatic elastase (PPE) and lipopolysaccharide (LPS) administration.

Results: We showed that isolated mouse and human DASCs could be indefinitely expanded and were able to further differentiate into mature alveolar structures in vitro. Single-cell analysis indicated that the engrafted cells expressed typical cellular markers of type I alveolar cells as well as the specific secreted proteins. Interestingly, transplantation of human DASCs derived from COPD patients into the lungs of NOD-SCID mice with COPD injury repaired the tissue damage and improved the pulmonary function.

Conclusions: The findings demonstrated that functional lung structure could be reconstituted by intrapulmonary transplantation of DASCs, suggesting a potential therapeutic role of DASCs transplantation in treatment for chronic obstructive pulmonary disease.
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http://dx.doi.org/10.1111/cpr.13046DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8168420PMC
June 2021

Emulsion-template synthesis of mesoporous nickel oxide nanoflowers composed of crossed nanosheets for effective nitrogen reduction.

Dalton Trans 2021 May;50(17):5835-5844

College of Chemical Engineering, Zhejiang University of Technology, Hangzhou 310014, China.

A novel emulsion-template synthesis approach was developed for the preparation of nickel oxide nanoflowers (NiO-NFs) composed of crossed mesoporous nanosheets. The interface assembly process was regulated by tuning the dosage of NH3·H2O, resulting in the tunability of thickness and size of mesoporous NiO nanosheets. The as-prepared NiO-NFs were characterized by field emission scanning electron microscopy, transmission electron microscopy, Brunauer-Emmett-Teller analysis, X-ray diffraction, and X-ray photoelectron spectroscopy (XPS). The results indicate that NiO-NFs have a mesopore size of about 9.5-15 nm and a crossed nanosheet thickness of about 12.4-50 nm. XPS results demonstrated that all NiO-NF samples consisted of Ni2+ and Ni3+. Electrochemical nitrogen reduction reaction (NRR) measurements revealed that NiO-NF-3.0 showed an optimal NRR performance of NH3 yield and faradaic efficiency (16.16 μg h-1 mg-1cat. and 9.17% at -0.4 V vs. RHE) in 0.1 M Na2SO4. Interestingly, NiO-NF-3.0 also displayed the highest Ni3+ content, which correlates with the order of electrochemical NRR performance. This can be attributed to the fact that Ni3+ promotes the electropositivity of NiO-NFs, resulting in more facile adsorption of N2 gas than Ni2+, and leading to enhanced electrocatalytic properties. These enhanced NRR performances are comparable or superior to those of reported noble-metal catalysts. This study provides a novel method for the fabrication of low-cost metal oxide nanomaterials that allows the construction of electrochemical NRR catalysts to meet the needs of industrial production. Also, it provides a new approach to improve the electrochemical properties by increasing the content of high-valent metal ions in a metal oxide.
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http://dx.doi.org/10.1039/d1dt00213aDOI Listing
May 2021

Research Progress on the SERPINE1 Protein and Chronic Inflammatory Diseases of the Upper Respiratory Tract: A Literature Review.

Int Arch Allergy Immunol 2021 May 4:1-6. Epub 2021 May 4.

Department of Otolaryngology, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China.

SERPINE1 protein is one important member of the serine proteinase inhibitor E superfamily that plays a crucial role in the fibrinolytic system. It has been identified which is related to chronic inflammatory lung diseases like allergic asthma and lung fibrosis. Recently, researchers have focused on the impact of SERPINE1 and its genetic polymorphisms on inflammatory diseases of the upper respiratory tract. In this review, we conclude that SERPINE1 is widely involved in the pathological process of chronic rhinosinusitis and allergic rhinitis (AR) and may play a pivotal role in tissue remodelling in chronic rhinosinusitis without nasal polyps. It is also found that the 4G allele of SERPINE1 gene is associated with the risk of upper respiratory diseases. More studies are needed to further clarify how SERPINE1 influences chronic rhinosinusitis and AR, which would be conducive to improving the therapeutic efficacy of treatments for upper respiratory diseases.
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http://dx.doi.org/10.1159/000516195DOI Listing
May 2021

Subjective Distress about COVID-19 and Its Social Correlates: Empirical Evidence from Hubei Province of China.

Authors:
Min Zhou Wei Guo

J Affect Disord 2021 06 20;289:46-54. Epub 2021 Apr 20.

Department of Social Work and Social Policy, School of Social and Behavioral Sciences, Nanjing University, Jiangsu Province, 210023, P. R. China; The Centre for Asia-Pacific Development Studies, Nanjing University, Jiangsu Province, 210023, P. R. China. Electronic address:

Background: The COVID-19 pandemic generates negative psychological effects such as distress. Social influences on subjective distress associated with COVID-19 remain understudied in the Chinese context. Wuhan with its surrounding areas in Hubei province was not only the locale where first COVID-19 cases were detected in the world but was also the hardest hit across China. Data from Hubei provide a unique opportunity to investigate COVID-19-related subjective distress and its social correlates.

Methods: We use original data (N=3,465) from the General Social Survey on COVID-19 in Hubei, China, conducted in August 2020. Regression analysis is employed to examine the impact of socioeconomic status, family structure, and social policies on COVID-19-related subjective distress measured by the Impact of Event-Scale-Revised (IES-R).

Results: First, individuals with higher socioeconomic status are not more immune to distress, and actually it is those better-educated ones who are more distressed. Second, family structure influences distress. Divorced and widowed individuals are more prone to distress than those who are married or single. Those living with COVID-19-infected family members or living with a larger family are particularly more distressed. Third, stricter lockdown measures promote real and perceived protection and also increase individuals' psychological distance from the disease, thereby reducing subjective distress.

Limitations: The sample is not totally random so we should use caution when generalizing the findings to the general population.

Conclusions: The findings contribute to our understanding of mental health disparity during the COVID-19 pandemic. Certain social groups are at a higher risk of distress than others.
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http://dx.doi.org/10.1016/j.jad.2021.04.026DOI Listing
June 2021

An overview of therapeutic potential of N-alkylated 1-deoxynojirimycin congeners.

Carbohydr Res 2021 Jun 16;504:108317. Epub 2021 Apr 16.

School of Chemical Engineering, Nanjing University of Science & Technology, Nanjing, Jiangsu, 210094, PR China. Electronic address:

Polyhydroxylated alkaloids display a wide range of biological activities, suggesting their use in the treatment of various diseases. Their most famous representative, 1-deoxynojirimycin (DNJ), is a natural product that shows α- and β-glucosidase inhibition. This molecule has been since converted into two clinically approved drugs i.e., Zavesca® and Glyset®, targeting type I Gaucher's disease and type II diabetes mellitus, respectively. This review examines the therapeutic potential of important DNJ congeners reported in last decade and presents concise mechanism of glycosidase inhibition. A brief overview of substituents conjugation's impact on DNJ scaffold (including N-alkylated DNJ derivatives, mono-valent, di-valent and multivalent DNJ congeners, N-[5-(adamantan-1-yl-methoxy)-pentyl]-1-deoxynojirimycin (AMP-DNM) look alike DNJ based lipophilic derivatives, AMP-DNM based neoglycoconjugates, DNJ click derivatives with varying carboxylic acids and aromatic moieties, conjugates of DNJ and glucose, and N-bridged DNJ analogues) towards various enzymes such as α/β glucosidase, porcine trehalase, as F508del-CFTR correctors, α-mannosidase, human placental β-glucocerebrosidase, N370S β-GCase, α-amylase and insect trehalase as potent and selective inhibitors have been discussed with potential bioactivities, which can provide inspiration for future studies.
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http://dx.doi.org/10.1016/j.carres.2021.108317DOI Listing
June 2021