Publications by authors named "Min Zhang"

6,018 Publications

  • Page 1 of 1

SARS-CoV-2-specific T cells associate with inflammation and reduced lung function in pulmonary post-acute sequalae of SARS-CoV-2.

PLoS Pathog 2022 May 26;18(5):e1010359. Epub 2022 May 26.

Department of Medicine, University of Colorado Anschutz Medical Campus, Aurora, Colorado, United States of America.

As of January 2022, at least 60 million individuals are estimated to develop post-acute sequelae of SARS-CoV-2 (PASC) after infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). While elevated levels of SARS-CoV-2-specific T cells have been observed in non-specific PASC, little is known about their impact on pulmonary function which is compromised in the majority of these individuals. This study compares frequencies of SARS-CoV-2-specific T cells and inflammatory markers with lung function in participants with pulmonary PASC and resolved COVID-19 (RC). Compared to RC, participants with respiratory PASC had between 6- and 105-fold higher frequencies of IFN-γ- and TNF-α-producing SARS-CoV-2-specific CD4+ and CD8+ T cells in peripheral blood, and elevated levels of plasma CRP and IL-6. Importantly, in PASC participants the frequency of TNF-α-producing SARS-CoV-2-specific CD4+ and CD8+ T cells, which exhibited the highest levels of Ki67 indicating they were activity dividing, correlated positively with plasma IL-6 and negatively with measures of lung function, including forced expiratory volume in one second (FEV1), while increased frequencies of IFN-γ-producing SARS-CoV-2-specific T cells associated with prolonged dyspnea. Statistical analyses stratified by age, number of comorbidities and hospitalization status demonstrated that none of these factors affect differences in the frequency of SARS-CoV-2 T cells and plasma IL-6 levels measured between PASC and RC cohorts. Taken together, these findings demonstrate elevated frequencies of SARS-CoV-2-specific T cells in individuals with pulmonary PASC are associated with increased systemic inflammation and decreased lung function, suggesting that SARS-CoV-2-specific T cells contribute to lingering pulmonary symptoms. These findings also provide mechanistic insight on the pathophysiology of PASC that can inform development of potential treatments to reduce symptom burden.
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http://dx.doi.org/10.1371/journal.ppat.1010359DOI Listing
May 2022

First report of causing rhizome rot on ginger in China.

Plant Dis 2022 May 25. Epub 2022 May 25.

Shandong Academy of Agricultural Sciences, 74641, Vegetable Research Institute, Jinan, China;

Ginger ( Rosc.) is a herbal plant, widely grown in China for its medicinal and culinary purposes. In July 2020, a new rhizome rot disease was observed on ginger in Laiwu, Shandong Province, China. The disease symptoms were observed on both above-ground and underground plant parts. The above ground stems and leaves becoming withered and yellow, and water-soaked symptoms were observed on the collar region. The diseased rhizomes were poorly developed with brown lesion and eventually they would rot, without offensive odors. Disease incidence was estimated at approximately 5% across the survey area. To isolate the pathogen, tissues from 30 rhizomes were cut from the border between diseased and healthy tissue, surface sterilized in 75% alcohol for 15 s, soaked in 0.1% mercuric chloride for 1 min, washed with sterile distilled water three times, and plated on potato dextrose agar (PDA) at 25°C for 2-3 days. Twenty nine fungal isolates with similar morphological characteristics were obtained and pure cultures were obtained using single spore isolation. The colony of AQJ-1, a representative isolate, on PDA was cottony, fluffy, white, and beige coloration on the reverse side at first, and subsequently many black sporangia were produced. The sporangia were black, sub-globose, and 45.2-181.7 μm (n = 50) in diameter. The sporangiospores were unequal, globose or sub-globose, about 3.2-8.7 × 4.6-12.3μm (n = 50) in diameter. For the molecular characterization, genomic DNA was extracted by modified CTAB method (Niu et al., 2008). Internal transcribed spacer (ITS) region and translation elongation factor 1-alpha (EF-1α) gene were amplified using the primer pairs ITS1/ITS4 (White et al., 1990) and MEF10/MEF4 (Abe et al., 2007), respectively. The ITS and EF-1α sequences of isolate AQJ-1 were submitted to GenBank (MN606288 and MN735220, respectively). The BLASTn analysis of the sequences showed 99%-100% similarity to the sequences of strain CBS 120.12 (MH854609, AB281529, respectively). Therefore, based on morphological and molecular characteristics, isolate AQJ-1 was identified as R. oryzae. For pathogenicity tests, thirty ginger seedlings (Laiwu Big Ginger) were grown for 30 days in plastic pots and removed from the pots and the rhizomes washed in running tap water. The rhizomes of fifteen ginger seedlings were attached to a 7 mm agar disk from a plate containing 2-day-old mycelium, and the other fifteen seedlings were attached to agar disk without mycelium as control. Then the inoculated and control seedlings were planted in pots and were kept in separate chambers in a greenhouse at 25±2 °C. After 14 days, the same symptoms of rhizome rot were observed in all inoculated plants as previously described, and no symptoms were observed on the control plants. The pathogen was re-isolated from symptomatic tissues, and was identified as R. oryzae, which full-filled the Koch's postulates. To our knowledge, this is the first report of causing rhizome rot on ginger in China. This disease may pose a potential threat to ginger production in China.
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http://dx.doi.org/10.1094/PDIS-12-21-2630-PDNDOI Listing
May 2022

Effect of sea cucumber peptides on the immune response and gut microbiota composition in ovalbumin-induced allergic mice.

Food Funct 2022 May 25. Epub 2022 May 25.

China-Russia Agricultural Processing Joint Laboratory, Tianjin Agricultural University, Tianjin 300392, P. R. China.

The prevalence of food allergies has increased in Asian countries. The aim of this study was to determine the potential value of sea cucumber peptide (SCP) for anti-allergic therapeutics in terms of their effect on immune response and gut microbiota composition. Results exhibited that SCP could significantly improve the allergy symptoms caused by ovalbumin and could reduce the risk of IgE mediated allergic disorders, as well as repair the morphological damage in the colon. Flow cytometry analysis indicated that SCP could improve the ratio of CD4+/CD8+ T lymphocytes. 16S rRNA results indicated that SCP could differently impact the composition of microbiota. The relative abundances of Bacteroidetes and Firmicutes and the Bacteroidetes/Firmicutes ratio were altered in normal mice. When compared with the OVA treated group, the SCP treated groups showed an increase in the relative abundance of Lachnospiraceae, Muribaculaceae and Ruminococcaceae, and a decrease in Bacteroidaceae, Prevotellaceae, and Lactobacillaceae. These results demonstrate that SCP exhibits potential antiallergic activities in a mouse model of ovalbumin allergy by regulating intestinal microbiota diversity and upregulating the immune response of T lymphocyte subpopulations, which might provide important evidence that SCP can be developed into a novel functional food for inhibiting ovalbumin allergy.
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http://dx.doi.org/10.1039/d2fo00536kDOI Listing
May 2022

Washed microbiota transplantation reduces serum uric acid levels in patients with hyperuricaemia.

World J Clin Cases 2022 Apr;10(11):3401-3413

Department of Gastroenterology, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou 510030, Guangdong Province, China.

Background: Previous studies have found that hyperuricaemia (HUA) is closely related to intestinal flora imbalance.

Aim: The current study investigated the effects and safety of washed microbiota transplantation (WMT) on serum uric acid (SUA) levels in different populations.

Methods: A total of 144 patients who received WMT from July 2016 to April 2020 in the First Affiliated Hospital of Guangdong Pharmaceutical University and had SUA data before treatment were selected. Changes in SUA levels before and after treatment were retrospectively reviewed based on short-term and mid-term effects of WMT regimens. SUA levels measured in the last test within 3 mo after the first WMT represented the short-term effect, and SUA levels measured in the last test within 3-6 mo after the first WMT represented the mid-term effect. The patients were divided into an HUA group (SUA > 416 μM) and a normal uric acid (NUA) group (SUA ≥ 202 μM to ≤ 416 μM) based on pretreatment SUA levels.

Results: Average short-term SUA levels in the HUA group decreased after WMT (481.00 ± 99.85 546.81 ± 109.64 μM, = 32, < 0.05) in 25/32 patients and returned to normal in 10/32 patients. The short-term level of SUA reduction after treatment moderately correlated with SUA levels before treatment ( = 0.549, ² = 0.300, < 0.05). Average SUA levels decreased after the first and second courses of WMT (469.74 ± 97.68 540.00 ± 107.16 μM, = 35, and 465.57 ± 88.88 513.19 ± 78.14 μM, = 21, < 0.05). Short-term and mid-term SUA levels after WMT and SUA levels after the first, second and third courses of WMT were similar to the levels before WMT in the NUA group ( > 0.05). Only 1/144 patients developed mild diarrhea after WMT.

Conclusion: WMT reduces short-term SUA levels in patients with HUA with mild side effects but has no obvious effect on SUA levels in patients with NUA.
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http://dx.doi.org/10.12998/wjcc.v10.i11.3401DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9048544PMC
April 2022

Sterile alpha motif and histidine-aspartic acid domain-containing protein 1 expression and its relationship with T cell activation in human immunodeficiency virus/acquired immune deficiency syndrome patients with lung-spleen deficiency syndrome pattern.

J Tradit Chin Med 2022 Jun;42(3):451-457

Henan Key Laboratory of Viral Diseases Prevention and Treatment of Traditional Chinese Medicine, the First Affiliated Hospital of Henan University of Traditional Chinese Medicine, Zhengzhou 450000, China.

Objective: To investigate the relationship between antiviral restriction factor Sterile Alpha Motif and Histidine-Aspartic acid domain-containing protein 1 (SAMHD1) expression and T cell activation, furthermore, identifying objective indexes of lung-spleen deficiency symptom pattern.

Methods: We assessed the profile of T lymphocyte subsets, characteristics of SAMHD1 and human leukocyte antigen DR (HLA-DR) expression in lung-spleen deficiency patients. At the same time, people living with human immunodeficiency virus / acquired immune deficiency syndrome (HIV/AIDS) (PLWHA) without obvious clinical symptoms and healthy donors in this area were used as controls.

Results: Immunohematologic indexes lower CD4 count, lower CD4/CD8 ratio and higher SAMHD1 level were found in lung-spleen deficiency patients. Furthermore, we demonstrated a positive relationship between SAMHD1 and HLA-DR level as well as with interferon factor in lung-spleen deficiency syndrome and patients without obvious clinical signs and symptoms groups.

Conclusions: These data indicated the positive relationship between SAMHD1 and T cell activation which further elucidated the role of SAMHD1 in cellular immune response. Furthermore, combination of T lymphocyte subsets counts and SAMHD1 level may be used as clinical and biological reference basis for the differentiation and diagnosis of HIV / AIDS traditional Chinese medicine syndromes.
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http://dx.doi.org/10.19852/j.cnki.jtcm.20220408.004DOI Listing
June 2022

A miniature chemiluminescence spectrometric system induced by atmosphere microplasma jet to avoid using hydrogen peroxide and catalyst.

Spectrochim Acta A Mol Biomol Spectrosc 2022 May 18;279:121399. Epub 2022 May 18.

School of Control Engineering, Hebei Key Laboratory of Micro-Nano Precision Optical Sensing and Measurement Technology, Northeastern University at Qinhuangdao, Qinhuangdao 066004, China; College of Information Science and Engineering, Northeastern University, Shenyang 110819, China. Electronic address:

A miniature luminol chemiluminescence system based on atmosphere microplasma is proposed for detection without any catalysts. In our research, atmosphere microplasma jet is employed to oxidize luminol and produce chemiluminescence instead of HO. The transport of OH radicals to the plasma-liquid interface and induce the chemiluminescence. The weight of the system is only 3.6 kg (including a 1.2 kg laptop), and the power consumption of the microplasma is only 0.045 W. The mechanism of luminol chemiluminiscence induced by microplasma jet and generation of microplasma jet are investigated in this study. A 1 mL sample solution is sufficient for trace 3-NPA determination within an analysis time of 6 min. In the range of 0.03-10 mg L, 3-NPA can be quantitatively analyzed along with a detection limit of 0.008 mg L. In addition, the proposed system is employed for real-world samples detection, including water samples, brown sugar and tainted sugarcane, which demonstrates the reliability and practical feasibility of the detection method.
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http://dx.doi.org/10.1016/j.saa.2022.121399DOI Listing
May 2022

Quality of death among older adults in China: The role of medical expenditure and timely medical treatment.

J Aging Soc Policy 2022 May 24:1-16. Epub 2022 May 24.

Dean's Professor in Global Health, Rory Meyers College of Nursing, New York University, NY, USA.

Medical expenses in the last year of life consume a large portion of healthcare expenditures, yet little is known about the relationship between medical expenditures in the last year of life and quality of death. Few empirical studies have investigated the association between timely medical treatment before dying and quality of death. This study aimed to examine the associations between medical expenditures in the last year of life, timely medical treatment before dying, and quality of death. Data derived from the Chinese Longitudinal Healthy Longevity Survey (CLHLS), the largest national representative study of the oldest-old in China. Results from multinomial logistic regression suggested that higher medical expenses in the last year of life and lack of timely medical treatment before dying are associated with lower quality of death. These findings highlight an urgent need for strengthening education on death and dying, developing hospice and palliative care services, and improving pain management at the end of life in China.
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http://dx.doi.org/10.1080/08959420.2022.2079907DOI Listing
May 2022

Effects of different low temperature pretreatments on properties of corn stover biochar for precursors of sulfonated solid acid catalysts.

Bioresour Technol 2022 May 19:127342. Epub 2022 May 19.

Faculty of Engineering, Shenyang Agricultural University, Shenyang 110866, China. Electronic address:

In this study, the effects of different pretreatment methods including phosphoric acid (PA), freeze drying (FD) and phosphoric acid-freeze drying combined (PA-FD) pretreatment on corn stover characteristics and pyrolysis of corn stover samples was investigated. The results demonstrated that the physiochemical properties of biochars varied significantly. In comparison, PA pretreatment could effectively remove a large portion of inorganics and improve the fuel characteristics. PA-CSB-600 had a greater HHV, lower O/C and H/C ratios, and a lower biochar energy yield (Y), indicating the possibility for an attractive fuel source. PA-FD pretreatment would significantly affected cell volume and caused mechanical damage to corn stover structure. As a sulfonated solid acid catalyst precursor, the results of cellulose catalytic hydrolysis indicated that the density of -SOH in FD-CSA was much higher than PA-FD-CSA, but lower surface special area. Specifically, PA-FD-CSB prepared at 600°C resulted in the maximum increase of cellulose conversion by 34.7-81.3%.
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http://dx.doi.org/10.1016/j.biortech.2022.127342DOI Listing
May 2022

Single Cell Dissection of Epithelial-Immune Cellular Interplay in Acute Kidney Injury Microenvironment.

Front Immunol 2022 4;13:857025. Epub 2022 May 4.

Department of Nephrology, First Medical Center of Chinese People's Liberation Army (PLA) of China General Hospital, Nephrology Institute of the Chinese People's Liberation Army, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing Key Laboratory of Kidney Disease Research, Beijing, China.

Background: Understanding the acute kidney injury (AKI) microenvironment changes and the complex cellular interaction is essential to elucidate the mechanisms and develop new targeted therapies for AKI.

Methods: We employed unbiased single-cell RNA sequencing to systematically resolve the cellular atlas of kidney tissue samples from mice at 1, 2 and 3 days after ischemia-reperfusion AKI and healthy control. The single-cell transcriptome findings were validated using multiplex immunostaining, western blotting, and functional experiments.

Results: We constructed a systematic single-cell transcriptome atlas covering different AKI timepoints with immune cell infiltration increasing with AKI progression. Three new proximal tubule cells (PTCs) subtypes (PTC-S1-new/PTC-S2-new/PTC-S3-new) were identified, with upregulation of injury and repair-regulated signatures such as Sox9, Vcam1, Egr1, and Klf6 while with downregulation of metabolism. PTC-S1-new exhibited pro-inflammatory and pro-fibrotic signature compared to normal PTC, and trajectory analysis revealed that proliferating PTCs were the precursor cell of PTC-S1-new, and part of PTC-S1-new cells may turn into PTC-injured and then become fibrotic. Cellular interaction analysis revealed that PTC-S1-new and PTC-injured interacted closely with infiltrating immune cells through CXCL and TNF signaling pathways. Immunostaining validated that injured PTCs expressed a high level of TNFRSF1A and Kim-1, and functional experiments revealed that the exogenous addition of TNF-α promoted kidney inflammation, dramatic injury, and specific depletion of TNFRSF1A would abrogate the injury.

Conclusions: The single-cell profiling of AKI microenvironment provides new insight for the deep understanding of molecular changes of AKI, and elucidates the mechanisms and developing new targeted therapies for AKI.
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http://dx.doi.org/10.3389/fimmu.2022.857025DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9114878PMC
May 2022

Correction: LncRNA AFAP1-AS1 promotes growth and metastasis of cholangiocarcinoma cells.

Oncotarget 2022 19;13:694. Epub 2022 May 19.

Department of Biliary-Pancreatic Surgery, Affiliated Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

[This corrects the article DOI: 10.18632/oncotarget.16880.].
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http://dx.doi.org/10.18632/oncotarget.28216DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9119686PMC
May 2022

Association Between Systemic Lupus Erythematosus and Cancer Morbidity and Mortality: Findings From Cohort Studies.

Front Oncol 2022 4;12:860794. Epub 2022 May 4.

Department of Infectious Diseases, First Affiliated Hospital, Army Medical University, Chongqing, China.

Background: Observational studies suggested that systemic lupus erythematosus (SLE) might be associated with increased cancer incidence and cancer-related death, however, the results are inconsistent. We aim to comprehensively estimate the causal relationships between SLE and cancer morbidity and mortality using a meta-analysis of cohort studies and Mendelian randomization.

Methods: A systematic search was conducted using PubMed to identify cohort studies published before January 21, 2021. Meta-analysis was performed to calculate relative risk (RR) and corresponding 95% confidence intervals (CI). In addition, we further evaluated the potentially causal relationships identified by cohort studies using two-sample Mendelian randomization.

Results: A total of 48 cohort studies involving 247,575 patients were included. We performed 31 main meta-analysis to assess the cancer risk and three meta-analyses to evaluate cancer mortality in SLE patients. Through meta-analyses, we observed an increased risk of overall cancer (RR=1.62, 95%CI, 1.47-1.79, <0.001) and cancer-related death (RR=1.52, 95%CI, 1.36-1.70, <0.001) in patients with SLE. Subgroup analysis by site-specific cancer showed that SLE was a risk factor for 17 site-specific cancers, including six digestive cancers (esophagus, colon, anus, hepatobiliary, liver, pancreatic), five hematologic cancers (lymphoma, Hodgkin's lymphoma, non-Hodgkin lymphoma, leukemia, multiple myeloma), as well as cancer in lung, larynx, cervical, vagina/vulva, renal, bladder, skin, and thyroid. In addition, further mendelian randomization analysis verified a weakly association between genetically predisposed SLE and lymphoma risk (odds ratio=1.0004, =0.0035).

Conclusions: Findings from our study suggest an important role of SLE in carcinogenesis, especially for lymphoma.

Systematic Review Registration: https://www.crd.york.ac.uk/PROSPERO/, CRD42021243635.
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http://dx.doi.org/10.3389/fonc.2022.860794DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9115099PMC
May 2022

Functional analysis of long noncoding RNAs involved in alkaline stress responses in wheat.

J Exp Bot 2022 May 20. Epub 2022 May 20.

Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Science, Shandong University, Qingdao 266237, China.

Saline-alkali soil is a key environmental problem affecting crop productivity. One of the most effective approaches to combat it is to breed stress-tolerant plants through genetic engineering. Shanrong No. 4 (SR4) is an alkaline-tolerant bread wheat cultivar derived from asymmetric somatic hybridization between the common wheat cultivar Jinan 177 (JN177) and tall wheatgrass. We aimed to explore the structure and function of alkalinity stress-responsive long noncoding RNAs (lncRNAs) in wheat. In this study, lncRNA sequencing was employed to identify stress tolerance-associated lncRNAs and their corresponding targets. Approximately 19,000 novel lncRNA sequences were detected in SR4 and JN177. Upon exposure to alkaline stress, SR4 differentially expressed 5691 lncRNAs, whereas JN177 differentially expressed 5932 lncRNAs. We selected five differentially expressed lncRNAs (L0760, L6247, L0208, L2098 and L3065) and generated transiently knocked down strains of wheat seedlings using the virus-induced gene silencing method. L0760 and L2098 knockdown caused the plants to exhibit sensitivity to alkaline stress, whereas L6247, L0208 and L3065 knockdown increased the ability of plants to tolerate alkaline stress. lncRNA-miRNA-target mRNA network and alkali-response-related lncRNA-target mRNA association networks were constructed to analyze the function of lncRNAs. Collectively, our results demonstrate that lncRNAs may have different roles under alkaline stress conditions.
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http://dx.doi.org/10.1093/jxb/erac211DOI Listing
May 2022

Effect of testing protocol and within-pen prevalence on the detection of Mycoplasma hyopneumoniae DNA in oral fluid samples.

Prev Vet Med 2022 May 13;204:105670. Epub 2022 May 13.

Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, IA, USA.

Combinations of 2 nucleic acid extractions and 3 Mycoplasma hyopneumoniae (MHP) PCRs (namely Protocol 1, 2, 3, and 4) were compared in terms of the probability of detecting DNA in pen-based oral fluid samples as a function of within-pen MHP prevalence. Oral fluid samples were created by randomly assigning 39 7-week old pigs to one of 5 pens, i.e., negative control pen (3 pigs) and 4 pens of 9 pigs each that differed in the proportion of MHP-inoculated pigs (1, 3, 6, or 9). Deep tracheal swabs were collected twice weekly to establish individual pig MHP infection status and derive within-pen prevalence estimation. On DPI 3, tracheal swabs from 15 of 19 inoculated pigs were MHP DNA positive. Oral fluids (n = 320) were collected daily from - 4 to 59 days post inoculation (DPI). Using a piecewise exponential model to account for within-pen transmission dynamics followed by a mixed-effect logistic regression, the probability of detecting MHP DNA in oral fluids was positively associated with within-pen prevalence (P < 0.0001) and differed among test protocols. MHP DNA was detected in 173 oral fluid samples with Protocol 3 versus 148, 134, and 101 with Protocols 4, 2, and 1, respectively. At 100% within-pen prevalence, the probability of detecting MHP DNA in oral fluids was highest using Protocol 3 (95.7%), followed by Protocols 4 (70.1%), 2 (60.1%), and 1 (34.0%). The fact that PCR protocols performed differently suggests that further improvements in extraction methods and MHP PCRs are possible. In the field, the dynamics of MHP infections should be taken into account if using oral fluid samples in surveillance.
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http://dx.doi.org/10.1016/j.prevetmed.2022.105670DOI Listing
May 2022

Boosting Li-Ion Diffusion Kinetics of NaTiMoO via Coherent Dimensional Engineering and Lattice Tailoring: An Alternative High-Rate Anode.

ACS Nano 2022 May 20. Epub 2022 May 20.

State Key Laboratory of Solidification Processing, Center for Nano Energy Materials, School of Materials Science and Engineering, Shaanxi Joint Laboratory of Graphene, Northwestern Polytechnical University, Xi'an 710072, P. R. China.

Featured with an exposed active facet, favorable ion diffusion pathway, and tailorable interfacial properties, low-dimensional structures are extensively explored as alternative electroactive materials with game-changing redox properties. Through a stepwise "proton exchange-insertion-exfoliation" procedure, in this article, we develop NaTiMoO (NTMO) nanosheets with weakened out-of-plane bonding and in-plane Mo doping of the tunnel structure. Real-time phase tracking of the laminated NTMO structures upon the lithiation/delithiation process suggests mitigated lattice variation; meanwhile, the kinetics simulation shows a mitigated Li-ion diffusion barrier along the [010] orientation. At an industrial-level areal capacity loading (2.5 mAh cm), the NTMO electrode maintains robust cycling endurance (91% capacity retention for 2000 cycles) even at 40 C, as well as the high energy/power densities in the as-constructed NTMO||LiFePO full cell prototype. The dimensional and lattice modifications presented in this study thus encourage further exploration of the tailored cation diffusion pathway for the construction of fast-charging batteries.
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http://dx.doi.org/10.1021/acsnano.2c01200DOI Listing
May 2022

Effects of physical exercise on muscle metabolism and meat quality characteristics of Mongolian sheep.

Food Sci Nutr 2022 May 1;10(5):1494-1509. Epub 2022 Mar 1.

College of Food Science and Engineering Inner Mongolia Agriculture University Hohhot China.

The objective of this study was to investigate the effects of exercise training on muscle metabolism, fatty acid composition, carcass traits, and meat quality characteristics of Mongolian sheep. Fourteen Mongolian sheep were randomly divided into two groups (7 sheep in each) and placed in two adjacent livestock pens. One group of sheep was kept in the pen (Control [C] group) and the other group of sheep (Training [T] group) were driven away in a field to walk twice a day. The results showed a reduction in pH measured 45 min post mortem, L*, a*, and b* value, intramuscular fat, and carcass length, and an increase in the ultimate pH value and shear force in the meat of T group in comparison with that of C group (< .050). Also, exercise training moderately affected the fatty acid composition of LT muscle. Compared with C group, the concentrations of myristoleic acid (C14:1) and stearic acid (C18:0) were increased (< .050), while the concentrations of C20:3 n-6, neurolic acid (C24:1), and n-3 polyunsaturated fatty acid (PUFA) were decreased in T group (< .050). Transcriptome analysis highlighted 621 genes differentially expressed in two groups, including 385 were up-regulated (e.g., and ) and 236 were down-regulated (e.g., and ) in T with respect to C group. Besides, considering these genes, a number of enrichment pathways related to muscle metabolic processes, involving carbohydrate metabolism, lipid metabolism, oxidation reduction process, and muscle tissue development, were highlighted. In conclusion, these results contributed to a better understanding of the possible biological and molecular processes underlying the effects of exercise training on muscle metabolism and meat quality in Mongolian sheep, and provide useful information for contributing to understand the phenotypic and functional differences in meat quality of sheep.
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http://dx.doi.org/10.1002/fsn3.2768DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9094461PMC
May 2022

Engineered Chimeric Peptides with IGF-1 and Titanium-Binding Functions to Enhance Osteogenic Differentiation In Vitro under T2DM Condition.

Materials (Basel) 2022 Apr 26;15(9). Epub 2022 Apr 26.

State Key Laboratory of Military Stomatology, National Clinical Research Center for Oral Diseases, Shaanxi International Joint Research Center for Oral Diseases, Department of General Dentistry and Emergency, School of Stomatology, Air Force Medical University, Xi'an 710032, China.

Due to the complexity of the biomolecules and titanium (Ti) combination, it is a challenge to modify the implant surface with biological cytokines. The study proposed a new method for immobilizing cytokines on implant surface to solve the problem of low osseointegration under type 2 diabetes mellitus (T2DM) condition. This new modified protein that connected Ti-binding artificial aptamer minTBP-1 with Insulin-like growth factor I (IGF-I), had a special strong affinity with Ti and a therapeutic effect on diabetic bone loss. According to the copies of minTBP-1, three proteins were prepared, namely minTBP-1-IGF-1, 2minTBP-1-IGF-1 and 3minTBP-1-IGF-1. Compared with the other modified proteins, 3minTBP-1-IGF-1 adsorbed most on the Ti surface. Additionally, this biointerface demonstrated the most uniform state and the strongest hydrophilicity. In vitro results showed that the 3minTBP-1-IGF-1 significantly increased the adhesion, proliferation, and mineralization activity of osteoblasts under T2DM conditions when compared with the control group and the other modified IGF-1s groups. Real-time PCR assay results confirmed that 3minTBP-1-IGF-1 could effectively promote the expression of osteogenic genes, that is, ALP, BMP-2, OCN, OPG, and Runx2. All these data indicated that the 3minTBP-1-IGF-1 had the most efficacious effect in promoting osteoblasts osteogenesis in diabetic conditions, and may be a promising option for further clinical use.
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http://dx.doi.org/10.3390/ma15093134DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9105221PMC
April 2022

Feature Optimization Method of Material Identification for Loose Particles Inside Sealed Relays.

Sensors (Basel) 2022 May 7;22(9). Epub 2022 May 7.

Reliability Institute for Electric Apparatus and Electronics, Harbin Institute of Technology, Harbin 150001, China.

Existing material identification for loose particles inside sealed relays focuses on the selection and optimization of classification algorithms, which ignores the features in the material dataset. In this paper, we propose a feature optimization method of material identification for loose particles inside sealed relays. First, for the missing value problem, multiple methods were used to process the material dataset. By comparing the identification accuracy achieved by a Random-Forest-based classifier (RF classifier) on the different processed datasets, the optimal direct-discarding method was obtained. Second, for the uneven data distribution problem, multiple methods were used to process the material dataset. By comparing the achieved identification accuracy, the optimal min-max standardization method was obtained. Then, for the feature selection problem, an innovative multi-index-fusion feature selection method was designed, and its superiority was verified through several tests. Test results show that the identification accuracy achieved by RF classifier on the dataset was improved from 59.63% to 63.60%. Test results of ten material verification datasets show that the identification accuracies achieved by RF classifier were greatly improved, with an average improvement of 3.01%. This strongly promotes research progress in loose particle material identification and is an important supplement to existing loose particle detection research. This is also the highest loose particle material identification accuracy achieved to in aerospace engineering, which has important practical value for improving the reliability of aerospace systems. Theoretically, it can be applied to feature optimization in machine learning.
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http://dx.doi.org/10.3390/s22093566DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9102643PMC
May 2022

Restorative biodegradable two-layered hybrid microneedles for melanoma photothermal/chemo co-therapy and wound healing.

J Nanobiotechnology 2022 May 19;20(1):238. Epub 2022 May 19.

State Key Laboratory of Oral Diseases, National Clinical Research Centre for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, People's Republic of China.

Tumor killing and wound healing are two complementary and influential processes during the treatment of melanoma. Herein, a two-layered microneedle platform was developed with bifunctional effect of chemo-photothermal synergistic melanoma therapy and skin regeneration. The bifunctional platform composed of embeddable curcumin nanodrugs/new Indocyanine Green/hyaluronic acid (Cur NDs/IR820/HA) microneedles and sodium alginate/gelatin/hyaluronic acid (SA/Ge/HA) supporting backing layer was prepared through a two-step casting process. With uniform incorporation of curcumin nanodrugs and IR820, the microneedles exhibited excellent photothermal performance under external near-infrared (NIR) light stimulation and tumor co-therapy ability. Once the embeddable microneedles were inserted into skin, they would rapidly dissolve and activate drug release successfully for tumor treatment. Moreover, the SA/Ge/HA supporting backing layer was left behind to cover the wound and promote the proliferation of endothelial and fibroblasts cells for enhanced skin regeneration. The two-layered microneedles platform can simultaneously eliminate the tumor and accelerate wounding healing, which may be potentially employed as a competitive strategy for the treatment of melanoma.
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http://dx.doi.org/10.1186/s12951-022-01426-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9118597PMC
May 2022

Spatiotemporal propagating decadal signal of ocean heat content and thermocline depth identified in the tropical Pacific.

Sci Total Environ 2022 May 16:155972. Epub 2022 May 16.

First Institute of Oceanography, Key Laboratory of Marine Sciences and Numerical Modeling, Ministry of Natural Resources, Qingdao, China; Laboratory for Regional Oceanography and Numerical Modeling, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, China; Shandong Key Laboratory of Marine Sciences and Numerical Modeling, Qingdao, China. Electronic address:

Tropical Pacific decadal variability (TPDV) and its mechanisms are essential for understanding long-term variations in global climate. The spatiotemporal pattern of this decadal variation has yet to be clarified. Here, on the basis of observational data with the help of the adaptive data analysis method, we extracted and investigated the spatiotemporal evolution of the tropical Pacific decadal variability in upper ocean heat content (UOHC) and thermocline depth. The tropical decadal signal propagated eastward along the equator from the western Pacific to the eastern Pacific after the 1970s, with a speed of 4-5 cm s yielding a decadal oscillation of approximately 11-13 years. This decadal variability of the thermocline fluctuations (UOHC) was proven to be closely correlated with western wind anomalies since the 1970s and may have been induced by the regime shift of the Pacific decadal oscillation. These peaks of decadal signals corresponded well with the strong El Niño-Southern Oscillation (ENSO) events, reflecting nonlinear rectification of ENSOs on TPDV. Moreover, the TPDV showed a modulating signal on moderate and weak ENSO events.
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http://dx.doi.org/10.1016/j.scitotenv.2022.155972DOI Listing
May 2022

Serum Atrial Natriuretic Peptide, Promoter Methylation, and Cardiovascular Disease: A 10-year Follow-Up Study in Chinese Adults.

Glob Heart 2022 7;17(1):27. Epub 2022 Apr 7.

Department of Epidemiology, School of Public Health, Medical College of Soochow University, Suzhou, China.

Background: Atrial natriuretic peptide (ANP) has been associated with cardiovascular disease (CVD) and related risk factors, but the clinical application is limited and the underlying mechanisms are not very clear. Here, we aimed to examine whether proANP and its coding gene methylation were associated with CVD in the Chinese population.

Methods: Serum proANP and peripheral blood DNA methylation of natriuretic peptide A gene () promoter was quantified at baseline for 2,498 community members (mean aged 53 years, 38% men) in the Gusu cohort. CVD events were obtained during 10 years of follow-up. A competing-risks survival regression model was applied to examine the prospective associations of proANP and promoter methylation with incident CVD.

Results: During follow-up, 210 participants developed CVD events, 50 participants died from non-cardiovascular causes, and 214 participants were lost. Per 1-nmol/L increment of serum proANP was associated with a 22% (HR = 1.22, 95%CI: 1.03-1.44, = 0.025) higher risk of CVD during follow-up. Of the 9 CpG sites assayed, per 2-fold increment of DNA methylation at CpG3 (located at Chr1:11908299) was significantly associated with a half lower risk of CVD (HR = 0.50, 95%CI: 0.30-0.82, = 0.006). The gene-based analysis found that DNA methylation of the 9 CpGs at promoter as a whole was significantly associated with incident CVD ( < 0.05).

Conclusions: Increased proANP and hypomethylation at promoter at baseline predicted an increased future risk of CVD in Chinese adults. Aberrant DNA methylation of the gene may participate in the mechanisms of CVD.
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http://dx.doi.org/10.5334/gh.1116DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8992767PMC
May 2022

New ZW4864 Derivatives as Small-Molecule Inhibitors for the β-Catenin/BCL9 Protein-Protein Interaction.

ACS Med Chem Lett 2022 May 25;13(5):865-870. Epub 2022 Apr 25.

Drug Discovery Department, H. Lee Moffitt Cancer Center and Research Institute, Tampa, Florida 33612-9497, United States.

A series of 1-(3-(2-amino-2-oxoethoxy)phenyl)piperidine-3-carboxamide derivatives was reported as new small-molecule β-catenin/B-cell lymphoma 9 (BCL9) protein-protein interaction (PPI) inhibitors. Compounds - were discovered to inhibit the β-catenin/BCL9 PPI with = 0.85-2.7 μM. The effects of on the β-catenin/BCL9 PPI in cellular context were demonstrated by β-catenin/BCL9 pull-down inhibition and dose-dependent suppression of Wnt/β-catenin signal transactivation. Notably, compound is more potent than , a previously reported analogue, in modulating transcription and expression of β-catenin target genes and suppressing survival of β-catenin-dependent cancer cells. The cellular on-target efficacy of was demonstrated by β-catenin rescue experiments. Compound represents a promising starting point for further optimization of β-catenin/BCL9 PPI inhibitors.
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http://dx.doi.org/10.1021/acsmedchemlett.2c00068DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9109161PMC
May 2022

Characterization of aspartyl aminopeptidase from Schistosoma japonicum.

Acta Trop 2022 May 16;232:106519. Epub 2022 May 16.

National Institute of Parasitic Diseases, Chinese Center for Diseases Control and Prevention (Chinese Center for Tropical Diseases Research); National Health Commission of the People's Republic of China (NHC) Key Laboratory of Parasite and Vector Biology; World Health Organization (WHO) Collaborating Center for Tropical Diseases; National Center for International Research on Tropical Diseases, Shanghai 200025, China. Electronic address:

The tegument of schistosomes is the interface between the worm and the host environment. Some molecules distributed on the tegument participate in host-parasite interactions. Aspartyl aminopeptidase (AAP), identified on the tegument of Schistosoma japonicum (S. japonicum), facilitate protein turnover by acting in concert with other aminopeptidases. In this study, the gene encoding S. japonicum aspartyl aminopeptidase (SjAAP) was cloned, expressed and characterized. Quantitative real-time PCR analysis showed that SjAAP was expressed in all studied developmental stages. The transcript level was higher in 8, 14, 21, and 28 days old worms than the other detected stages. Moreover, the level of expression in 42-day-old male worms was significantly higher than that in females. The recombinant SjAAP (rSjAAP) was expressed as both supernatant and inclusion bodies in Escherichia coli BL21 cells. The enzymatic activity of rSjAAP was 4.45 U/mg. The Km and Vmax values for H-Asp-pNA hydrolysis were discovered to be 5.93 mM and 0.018 mM·min. Immunofluorescence analysis revealed that SjAAP is primarily distributed on the tegument and parenchyma of schistosomes. Western blot showed that rSjAAP possessed good immunogenicity. Although specific antibodies were produced in BALB/c mice vaccinated with rSjAAP emulsified with ISA 206 adjuvant, no significant reduction of worm burden and number of eggs in the liver was observed. Therefore, rSjAAP may not be suitable to act as a potential vaccine candidate against schistosomiasis japonica in mice. However, this study provides some foundation for further exploration of the biological function of this molecule.
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http://dx.doi.org/10.1016/j.actatropica.2022.106519DOI Listing
May 2022

Self-organization of zinc ions with a photosensitizer for enhanced antibiofilm and infected wound healing.

Nanoscale 2022 May 18. Epub 2022 May 18.

Joint Centre of Translational Medicine, Department of Orthopaedics, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province, 325000, P. R. China.

Antimicrobial materials have been developed to combat bacteria more effectively and promote infected wound healing. However, it is widely recognized that the potential toxic effects and complexity of the synthesis process hinder their practical applications. In this work, we introduced a strategy for fighting bacteria and promoting wound healing caused by () infection by the self-combination of Zn and clinically applied 5-aminolevulinic acid hydrochloride (ALA) in the microbes. The clinical ALA could target and accumulate in the biofilm as well as contribute to the low-dose Zn penetrating the biofilm due to the self-organized formation of Zn protoporphyrin IX . Upon exposing to a 635 nm laser, the self-combination of ALA and Zn significantly inhibited and eliminated the biofilm a synergistic biofilm eradication mechanism that enhanced photodynamic inactivation and aggravated cell wall/membrane disruption. In addition, the combination of ALA and Zn could accelerate wound repair and reduce inflammatory response without causing cytotoxicity. The proposed strategy in this study illustrates the clinical prospects of eradicating biofilms and repairing infected wounds and demonstrates good biocompatibility towards infectious diseases.
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http://dx.doi.org/10.1039/d2nr01404aDOI Listing
May 2022

Correction: Homogeneous and heterogeneous risk and prognostic factors for lung metastasis in colorectal cancer patients.

BMC Gastroenterol 2022 May 17;22(1):249. Epub 2022 May 17.

Department of Pharmacology, College of Pharmacy, Chongqing Medical University, 1 Yixueyuan Road, Yuzhong District, Chongqing, 400016, China.

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http://dx.doi.org/10.1186/s12876-022-02306-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9115934PMC
May 2022

Association of soluble suppression of tumorigenicity 2 with new-onset atrial fibrillation in acute myocardial infarction.

Cardiology 2022 May 17. Epub 2022 May 17.

Background The combination of acute myocardial infarction (AMI) and atrial fibrillation (AF) is still a thorny problem in the clinic. At present, there are few reports on the role of soluble suppression of tumorigenicity 2 (sST2) in AF after AMI. This study was to explore the predictive value of sST2 in patients with AMI for new-onset AF. Methods This is a single-center retrospective clinical observation study. We continuously included AMI patients from September 2019 to November 2021. The concentration of sST2 in blood samples was determined. During admission, suspicious heart rhythm was recorded by electrocardiogram (ECG) monitoring, and new-onset AF was confirmed by immediate body surface ECG. Results After multiple factors were included, age, right coronary artery (RCA), high sensitivity c-reactive protein (hs-CRP), left ventricular ejection fraction (LVEF), and sST2 were still risk factors for new-onset AF. The area under curve (AUC) value of age and sST2 was more than 0.7, which showed good diagnostic value. For re-evaluation, the sST2 was added to the clinical new-onset AF prediction model. It was found that the integrated discrimination improvement (IDI) and net reclassification index (NRI) in the model were improved significantly. Conclusion sST2 is an independent predictor of new-onset AF in patients with AMI and can improve the accuracy of the AF risk model.
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http://dx.doi.org/10.1159/000524765DOI Listing
May 2022

Efficacy and safety of drug-coated balloons in the treatment of coronary lesions in very small vessels: a prospective, multicenter, single-arm trial.

Ann Transl Med 2022 Apr;10(8):445

Department of Cardiology, the Affiliated Hospital of Zunyi Medical College, Zunyi, China.

Background: Evidence of the use of drug-coated balloons (DCB) in large or small coronary lesions and in-stent restenosis has accumulated over the past years. Due to their anatomical peculiarity, the treatment of very small vessels (VSV) (lumen diameter <2 mm) is still a controversial issue. Studies that examine the use of DCB in VSV are limited. We investigated the efficacy and safety of using DCBs for the coronary lesions in VSV undergoing percutaneous coronary intervention (PCI).

Methods: In this prospective, single-arm study, we enrolled adult patients with coronary artery disease from six centers in China. A total of 29 patients had VSV with a target lesion stenosis ≥70% were included. All patients were treated with DCB. The primary endpoint was late lumen loss (LLL) at 9 months of follow-up. The secondary endpoints were major adverse cardiac events including target lesion revascularization, death, or myocardial infarction at 9 months of follow-up.

Results: Twenty-nine eligible patients with VSV were enrolled between November 2019 to May 2020. Angiographic and clinical follow-up were completed at 9 months in 18 (56.25%) patients (7 patients refused to final angiography; 2 failed to finish DCB angioplasty; 1 patient request; 1 other causes of death). The mean diameter of the reference vessel of the target lesion was 1.71±0.27 mm, the minimum lumen diameter (MLD) of the target lesion before operation was 0.31±0.24 mm, the average LLL of the target lesion was 0.13±0.28 mm, and the MLD of the target vessel immediately after operation was (1.19±0.20 mm) and at the 9-month follow-up (1.06±0.31 mm) were significantly higher than those before operation (P=0.043). One patient (5.56%) underwent revascularization. No myocardial infarction or death occurred during follow-up after treatment with DCBs.

Conclusions: DCB can be a safe and effective alternative in the treatment of coronary lesions in VSV.
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http://dx.doi.org/10.21037/atm-22-1266DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9096399PMC
April 2022

Analysis of World-Scale Mitochondrial DNA Reveals the Origin and Migration Route of East Asia Goats.

Front Genet 2022 26;13:796979. Epub 2022 Apr 26.

College of Life Science and Agronomy, Zhoukou Normal University, Zhoukou, China.

Despite much attention on the history of goat evolution, information on origin, demographic history, and expansion route remains controversial. To address these questions, we collected 4,189 published goat DNA sequences including 1,228 sequences from 57 breeds in China and 2,961 sequences including 193 goat breeds from 71 other countries and carried out an integrated analysis. We found goat breeds from South China had the highest genetic diversity of lineage B, and subclades B2 only were found in Southwest China, suggesting that lineage B (particularly, subclade B2) probably originated from Southwest China and its surrounding areas. In addition, in this study, we found that lineage A from South China also presented higher genetic diversity and earlier expansion time (10, 606 years ago), even earlier than breeds from the Middle East. Hence, we speculated that South China and surrounding areas were the origin of lineage B and also the transportation hub for lineage A spreading to North China and Southwest Asia. Furthermore, according to the analysis of correlation between genetic differentiation value λ1 and λ2 and geographical distance, we further confirmed two phases of migration in goat breeds of North China. These results will contribute to a better understanding of the origin and migration history of domestic goat.
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http://dx.doi.org/10.3389/fgene.2022.796979DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9101309PMC
April 2022

Defective MNiFeO (M = Cu, Zn, Co, Mn) NRs derived from cation-exchanged FeNi-MOFs for catalytic nitroarene hydrogenation.

J Colloid Interface Sci 2022 May 6;623:63-76. Epub 2022 May 6.

Ministry Key Laboratory of Oil and Gas Fine Chemicals, School of Chemical Engineering and Technology, Xinjiang University, Urumqi 830046, China; Xinjiang De'an Environmental Protection Technologies Inc, Urumqi 830046, China.

Catalytic hydrogenation reduction provides a potential route to detoxify nitro compounds. Spinel NiFeO (NiFeO) serves as a natural abundance and low-cost catalyst but suffers from low catalytic activity due to poor redox nature and limited active sites. Herein, defective MNiFeO (M = Cu, Zn, Co, Mn) nanorods (NRs) were synthesized by pyrolysis of cation-exchanged FeNi-MOFs. The introduction of M modified the electronic structure of NiFeO and thus accelerated the electron transfer and proton transport in the hydrogenation of nitro aromatics. CuNiFeO exhibited the highest catalytic activity with a turn frequency of 2.89 × 10h, giving 308- and 422-fold larger than that of NiFeO-V and NiFeO in the reduction of 4-nitrophenol (4-NP). CuNiFeO NRs also presented exceptional performances in the reduction of the other nitroarenes. The remarkable improvement in activity of mesoporous CuNiFeO may be attributed to its ternary composites, and increased oxygen vacancies. This work not only provides a simple route to constructing mesoporous MNiFeO NRs with oxygen vacancies, but also further discusses the effects of metal component and oxygen defects in the catalytic reduction of 4-NP.
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http://dx.doi.org/10.1016/j.jcis.2022.04.177DOI Listing
May 2022

Mammalian eIF4E2-GSK3β maintains basal phosphorylation of p53 to resist senescence under hypoxia.

Cell Death Dis 2022 May 14;13(5):459. Epub 2022 May 14.

College of Life Science and Technology, College of Biomedicine and Health, Huazhong Agricultural University, Wuhan, 430070, China.

Hypoxia modulates senescence, but their physiological link remains unclear. Here, we found that eIF4E2, a hypoxia-activated translation initiation factor, interacted with GSK3β to maintain phosphorylation of p53, thus resisting senescence under hypoxia. RNA-binding protein RBM38 interacted with eIF4E to inhibit the translation of p53, but GSK3β-mediated Ser195 phosphorylation disrupted the RBM38-eIF4E interaction. Through investigation of RBM38 phosphorylation, we found that the eIF4E2-GSK3β pathway specifically regulated proline-directed serine/threonine phosphorylation (S/T-P). Importantly, peptides e2-I or G3-I that blocking eIF4E2-GSK3β interaction can inhibit the basal S/T-P phosphorylation of p53 at multiple sites, therby inducing senescence through transcriptional inhibition. Additionally, a nanobody was screened via the domain where eIF4E2 bound to GSK3β, and this nanobody inhibited S/T-P phosphorylation to promote senescence. Furthermore, hypoxia inhibited eIF4E2-GSK3β pathway by mediating S-Nitrosylation of GSK3β. Blocking eIF4E2-GSK3β interaction promoted liver senescence under hypoxia, thus leading to liver fibrosis, eventually accelerating N, N-diethylnitrosamine (DEN)-induced tumorigenesis. Interestingly, eIF4E2 isoforms with GSK3β-binding motif exclusively exist in mammals, which protect zebrafish heart against hypoxia. Together, this study reveals a mammalian eIF4E2-GSK3β pathway that prevents senescence by maintaining basal S/T-P phosphorylation of p53, which underlies hypoxia adaptation of tissues.
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http://dx.doi.org/10.1038/s41419-022-04897-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9107480PMC
May 2022

Akkermansia muciniphila and its outer membrane protein Amuc_1100 prophylactically attenuate 5-fluorouracil-induced intestinal mucositis.

Biochem Biophys Res Commun 2022 May 3;614:34-40. Epub 2022 May 3.

School of Life Sciences, Anhui University, Hefei, 230601, Anhui, China; Key Laboratory of Human Microenvironment and Precision Medicine of Anhui Higher Education Institutes, Anhui University, Hefei, 230601, Anhui, China. Electronic address:

5-Fluorouracil (5-FU) is a chemotherapy drug used to treat tumors. Previous studies have shown that Akkermansia muciniphila (A. muciniphila) and its outer membrane protein, Amuc_1100, alleviate dextran sodium sulfate (DSS)-induced colitis in mice. We investigated the effects of both A. muciniphila and Amuc_1100 on 5-FU-induced intestinal mucosal damage in mice. C57BL/6 mice were gavaged with A. muciniphila or Amuc_1100 daily before, during, and after 5-FU injection for a total of 14 days. By evaluating diarrheal toxicity scores, body weight changes, colonic anatomy images, and histopathology of intestinal injury in these mice, we found that A. muciniphila and Amuc_1100 alleviated 5-FU-induced intestinal mucositis. Quantitative polymerase chain reaction assays of intestinal cytokine mRNA levels demonstrated that both A. muciniphila and Amuc_1100 attenuated the upregulation of intestinal Tumor Necrosis Factor-α (TNF-α) and interleukin-6 (IL-6) induced by 5-FU treatment. In addition, they both reduced 5-FU-induced the NLR family pyrin domain containing 3 (NLRP3) inflammatory vesicle activation. Furthermore, by monitoring the mRNA expression of tight junction proteins in the intestine, we found that A. muciniphila and Amuc_1100 were capable of restoring the damaged intestinal barrier. Notably, A. muciniphila and Amuc_1100 also played a role in altering the structure of the intestinal microbial community. The present study revealed the protective role of both A. muciniphila and Amuc_1100 in the intestinal mucositis caused by 5-FU, providing new insights into treatment options.
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http://dx.doi.org/10.1016/j.bbrc.2022.04.135DOI Listing
May 2022
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