Publications by authors named "Min Yang"

3,150 Publications

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Mobile tigecycline resistance gene tet(X4) persists with different animal manure composting treatments and fertilizer receiving soils.

Chemosphere 2022 Aug 8:135866. Epub 2022 Aug 8.

State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China; University of Chinese Academy of Sciences, Beijing, 100049, China. Electronic address:

The emergence of plasmid-mediated tigecycline-resistant genes [tet (×3) to tet (×6)] in animals and humans has raised serious concerns over their possible cross-environmental dissemination. However, behavior of these emerging mobile tet(X)-variant genes in manure treatment processes, particularly for different composting treatments, has not yet been studied. Here, we explored the environmental behavior of mobile tet(X)-variant genes in two typical manure composting treatments and amended soils based on a large-scale molecular investigation across eight provinces in China. Results showed that tet (×4) was the predominant mobile tet(X)-variant gene in fresh manure, natural and thermophilic composting products with both the highest detection frequency (82.5% ± 14.7%), and absolute abundance of tet (×4) [4.26 ± 0.09) × 10] copies/g dry weight, followed by tet (×3), tet (×6), and tet (×5). The occurrence of all mobile tet(X)-variant genes, particularly tet (×4), in receiving soil following composting fertilizer application indicated their transmission from manure to soil. Paired-sampling strategy revealed no significant reduction in mobile tet(X)-variant genes by natural composting, while thermophilic composting exhibited clear efficacy in removing tet(X)-variant genes. After thermophilic composting, tet (×4) exhibited the lowest reduction (94.1%) compared with other mobile tet(X)-variant genes (96.9%-99.9%), which may be attributable to its significant correlation with ISCR2 (P < 0.05) facilitating its transfer to various hosts including persisted thermotolerant bacteria. Thus, tet (×4) showed better persistence in livestock-related environments. Collectively, this study highlights the importance of controlling the environmental dissemination of clinically relevant mobile tet(X)-variant genes by establishing a sound process and operational strategy.
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http://dx.doi.org/10.1016/j.chemosphere.2022.135866DOI Listing
August 2022

is transcriptionally activated by and suppresses hyperoxia-induced alveolar epithelial cell injury.

Exp Lung Res 2022 Aug 11:1-11. Epub 2022 Aug 11.

Department of Respiratory, Hunan Children's Hospital, Changsha, China.

Purpose: Bronchopulmonary dysplasia (BPD) is a long-term respiratory condition. More than a quarter of extremely premature newborns are harmed by BPD. At present, there are no apparent effective drugs or treatments for the condition. In this study, we aimed to investigate the functional role and mechanism of lymphoid enhancer-binding factor 1 () in BPD .

Materials And Methods: Blood samples from BPD patients and healthy volunteers were gathered, and an model of BPD was developed in alveolar epithelial cells (AECs) MLE-12 induced by hyperoxia. Then expression of krüppel-like factor 4 (/) and / were evaluated. After overexpressing plasmid and the vector were transfected into hyperoxia-induced MLE-12 cells, cell proliferation assays were carried out. Cell apoptosis was investigated by a flow cytometry assay, and apoptosis related proteins Bcl-2, cleaved-caspase 3 and 9 were analyzed by a western blot assay. The binding between and promoter predicted on the JASPAR website was verified using luciferase and ChIP assays. For further study of the mechanism of and in BPD, gain-of-function experiments were performed.

Results: The mRNA levels of / and / were diminished in clinical BPD serum samples and hyperoxia-induced MLE-12 cells. Overexpression of stimulated AEC proliferation and suppressed AEC apoptosis induced by hyperoxia. Mechanically, bound to 's promoter region and aids transcription. Moreover, the results of gain-of-function experiments supported that could impede AEC damage induced by hyperoxia via stimulating .

Conclusion: and expression levels were declined in hyperoxia-induced AECs, and could be transcriptionally activated by and protect against hyperoxia-induced AEC injury in BPD. As a result, might become a prospective therapeutic target for BPD.
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http://dx.doi.org/10.1080/01902148.2022.2108945DOI Listing
August 2022

Role and mechanism of the zinc finger protein ZNF580 in foam-cell formation.

Exp Ther Med 2022 Sep 19;24(3):579. Epub 2022 Jul 19.

Department of Cardiac Thoracic Surgery, Characteristic Medical Center of People's Armed Police Force, Tianjin 300309, P.R. China.

Coronary atherosclerotic heart disease poses a significant threat to human health. The pathological basis is atherosclerosis and foam-cell formation is the key factor in the initiation of atherosclerosis. In the present study, foam cell models were established using 50 ng/ml oxidized low-density lipoprotein to stimulate cultures of THP-1 cells for 72 h. The expression of zinc finger protein 580 (ZNF580), a Cys2-His2 zinc finger protein containing 172 amino acids that was originally cloned by screening a human aortic cDNA library, was measured in foam cells and its interaction with various regulatory factors during foam-cell formation was investigated. Oil red O staining was used to observe cell morphology and intracellular lipid levels. Lentivirus transfection was employed to either overexpress or silence ZNF580 in THP-1 cells, and an inverted fluorescence microscope was used to observe the distribution of ZNF580 immunofluorescence to determine the transfection rate. RNA and total protein were extracted and the expression levels of ZNF580, CD36, peroxisome proliferator-activated receptor-γ (PPAR-γ), ATP-binding cassette transporter A1 (ABCA1) and apolipoprotein E (ApoE) were measured by reverse transcription-quantitative PCR. The protein levels were examined by western blot analysis to evaluate the interaction between ZNF580 and associated regulatory factors. ZNF580 was able to significantly increase the expression levels of ApoE and ABCA1 and significantly decrease the expression levels of CD36 and PPAR-γ, suggesting that ZNF580-mediated inhibition of foam-cell formation is associated with the PPAR-γ-CD36 signalling pathway. Based on these findings, ZNF580 may be a potential therapeutic candidate for the treatment of coronary atherosclerotic heart disease.
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http://dx.doi.org/10.3892/etm.2022.11516DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9353537PMC
September 2022

miR-34a induces neutrophil apoptosis by regulating Cdc42-WASP-Arp2/3 pathway-mediated F-actin remodeling and ROS production.

Redox Rep 2022 Dec;27(1):167-175

Department of Gastroenterology, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, People's Republic of China.

Background: The number of neutrophils is significantly reduced in myelodysplastic syndrome (MDS), but the molecular basis remains unclear. We recently found that miR-34a was significantly increased in MDS neutrophils. Therefore, this study aims to clarify the effects of aberrant miR-34a expression on neutrophil counts.

Methods: miR-34a mimics/inhibitor transfection were performed in neutrophil-like differentiated HL60 (dHL60) cells, and a FACSCalibur flow cytometer was used to measure ROS production and apoptosis. In addition, the Cdc42-WASP-Arp2/3 pathway inhibitor (ML141) and activator (CN02) treated the dHL60 cells, and then ROS production, apoptosis and related proteins expression were detected. And, luciferase reporter assay to verify the relationship of miR-34a and the Cdc42-WASP-Arp2/3 pathway.

Results: overexpression of miR-34a could induce ROS production and apoptosis, decrease the expression levels of DOCK8, p-WASP, WASP, Arp2, Arp3, and increase F-actin's expression. Meanwhile, knockdown of miR-34a could decrease ROS production and apoptosis, increase the expression of DOCK8, p-WASP, WASP, Arp2, Arp3, and decrease F-actin's expression. Immunofluorescence staining showed aberrant miR-34a and Cdc42-WASP-Arp2/3 pathway could induce F-actin membrane transfer. Luciferase reporter assay indicated that DOCK8 was a direct target gene of miR-34a.

Conclusion: These data indicates miR-34a may induce neutrophil apoptosis by regulating Cdc42-WASP-Arp2/3 pathway-mediated F-actin remodeling and ROS production.
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http://dx.doi.org/10.1080/13510002.2022.2102843DOI Listing
December 2022

Beclin1 Deficiency Suppresses Epileptic Seizures.

Front Mol Neurosci 2022 22;15:807671. Epub 2022 Jul 22.

Chongqing Key Laboratory of Neurology, Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Epilepsy is a common disease of the nervous system. Autophagy is a degradation process involved in epilepsy, and in turn, seizures can activate autophagy. Beclin1 plays a critical role in autophagy and participates in numerous physiological and pathological processes. However, the mechanism underlying the effect of Beclin1 on epilepsy remains unclear. In this study, we detected increased expression of Beclin1 in brain tissues from patients with temporal lobe epilepsy (TLE). Heterozygous disruption of decreased susceptibility to epilepsy and suppressed seizure activity in two mouse epilepsy models. We further illustrated for the first time that heterozygous disruption of suppresses excitatory synaptic transmission, which may be caused by a decreased dendritic spine density. These findings suggest for the first time that the regulation of Beclin1 may serve as a strategy for antiepileptic therapy. In addition, Beclin1 participates in synaptic transmission, and the development of dendritic spines may be a biological function of Beclin1 independent of its role in autophagy.
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http://dx.doi.org/10.3389/fnmol.2022.807671DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354790PMC
July 2022

Isoimperatorin down-regulates epithelial mesenchymal transition through modulating NF-κB signaling and CXCR4 expression in colorectal and hepatocellular carcinoma cells.

Cell Signal 2022 Aug 4:110433. Epub 2022 Aug 4.

Department of Science in Korean Medicine, Kyung Hee University, 24 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Republic of Korea. Electronic address:

Worldwide, metastatic spread of tumor is the major cause of cancer-related deaths. There are many anti-metastatic agents that can target metastasis-related pathways, but there are relatively few studies on agents targeting C-X-C chemokine receptor type 4 (CXCR4) signaling axis. Here, we have investigated whether Isoimperatorin (IIPT), derived from Angelica dahurica can modulate CXCR4 signaling axis-related epithelial-to-mesenchymal transition (EMT) and tumor metastasis process. We evaluated the influence of IIPT on CXCR4, HER2, MMPs, EMT markers, and NF-κB signaling pathway by using Western blot analysis. The cellular invasion and migration were observed by Boyden chamber and wound healing assays. IIPT has a down-regulatory effect on CXCR4, HER2, and MMP-9/2. On the contrary, imperatorin (IPT) as compared to IIPT did not alter the expression of CXCR4. IIPT down-regulated the protein levels and RNA levels of mesenchymal markers, twist, snail, and enhanced the levels of different epithelial markers. IIPT also inhibited cell migration, invasion, and proliferation. Furthermore, IIPT negatively regulated constitutive NF-κB activation and inhibited the translocation of phospho-p65 and p65 into the nuclei. IIPT can potentially function as a novel anti-metastatic agent by inhibiting EMT and metastasis process via inhibition of NF-κB activation and CXCR4 expression in colorectal and hepatocellular carcinoma cells.
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http://dx.doi.org/10.1016/j.cellsig.2022.110433DOI Listing
August 2022

Silk protein/polyvinylpyrrolidone nanofiber membranes loaded with puerarin accelerate wound healing in mice by reducing the inflammatory response.

Biomater Adv 2022 Apr 22;135:212734. Epub 2022 Apr 22.

College of traditional Chinese Medicine, Jilin Agriculture Science and Technology College, Jilin 132101, China. Electronic address:

In modern clinical applications, wound healing remains a considerable challenge. Excessive inflammatory response is associated with delayed wound healing. In this study, we prepared composite nanofibrous membranes by mixing the Chinese herbal extract puerarin (PUE) with natural silk protein (SF) and synthetic polymer polyvinylpyrrolidone (PVP) using electrostatic spinning technique, and conducted a series of studies on the structural and biological properties of the fibrous membranes. The results showed that the loading of PUE increased the diameter, porosity and hydrophilicity of nanofibers, which were more favorable for cell adhesion and proliferation. ABTS radical scavenging assay also showed that the loading of PUE enhanced the antioxidant properties of the fibrous membranes. In addition, SF/PVP/PUE nanofibers are non-toxic and can be used as wound dressings. In vitro experiments showed that SF/PVP/PUE nanofibers could effectively alleviate lipopolysaccharide (LPS)-induced inflammation in Immortalized human keratinocytes (HaCaT) cells and down-regulate pro-inflammatory cytokine expression in cells. In vivo studies further showed that the SF/PVP/PUE nanofibers could effectively accelerate wound repair. The mechanism is that SF/PVP/PUE nanofibers can inhibit the activation and transduction of toll-like receptor 4/myeloid differentiation factor88/nuclear factor kappa B (TLR4/MyD88/NF-κB) and phosphatidylinositol 3-kinase/protein kinase B (PI3K/AKT) signaling pathways, thereby reducing the inflammatory response and achieving wound healing.
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http://dx.doi.org/10.1016/j.bioadv.2022.212734DOI Listing
April 2022

Developing dynamic speed limit strategies for mixed traffic flow to reduce collision risks at freeway bottlenecks.

Accid Anal Prev 2022 Aug 1;175:106781. Epub 2022 Aug 1.

Hunan Communication Research Institute, Changsha, Hunan 410075, China. Electronic address:

Connected and automated vehicles (CAVs) have the great potential to improve traffic flow because of their particular characteristics of connectivity and automation. This study aims to develop CAV control strategies based on car-following speed balance, which are defined as dynamic speed limit (DSL) strategies, and examine their performances on reducing freeway collision risks via microscopic simulations. The core idea of DSL strategy is to command the CAVs to slow down actively before reaching the bottlenecks, and form moving barriers to guide the following human driven vehicles to passively decelerate. Three DSL strategies are first developed for CAVs based on vehicle dynamics principles, and the influences of various position distribution patterns of CAVs on three strategies are compared in the one-lane scenario. Then, the DSL strategy with the best performance is selected based on simulation experiments, and a conventional variable speed limit control is used to compare the performance of our proposed methods. Finally, the DSL strategy based on Min control is tested in the two-lane, three-lane and four-lane scenarios to verify the effectiveness. Simulation results indicate that: (1) three DSL strategies based on CAVs can significantlyreduce collision risks when CAVs reach a certain proportion; (2) the uniform distribution of CAVs can maximize the effect of the moving barriers; (3) DSL strategy based on Min control is negatively affected by lane-changing behaviors, but still works well in the multi-lane scenario.
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http://dx.doi.org/10.1016/j.aap.2022.106781DOI Listing
August 2022

Selective halocyclization and iodosulfonylation of -benzothiazol-2-yl alkynamides under mild conditions.

Org Biomol Chem 2022 Aug 4. Epub 2022 Aug 4.

Key Laboratory of Biomaterials and Biofabrication in Tissue Engineering of Jiangxi Province, Key Laboratory of Biomedical Sensors of Ganzhou, School of Pharmacy, School of Basic Medicine, Gannan Medical University, Ganzhou, 341000, China.

The selective halocyclization and iodosulfonylation of -benzothiazol-2-yl alkynamides under mild conditions is described. An effective synthetic strategy to pyrimidobenzothiazoles a 6- halocyclization of -benzothiazol-2-yl alkynamides was developed at room temperature with a broad substrate scope. Furthermore, several multisubstituted α,β-enones were synthesized using the same starting materials.
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http://dx.doi.org/10.1039/d2ob01165dDOI Listing
August 2022

Genetic Variations in IFNGR1, BDNF and IL-10 May Predict the Susceptibility to Depression and Anxiety in Chinese Women With Breast Cancer.

Clin Breast Cancer 2022 Jul 9. Epub 2022 Jul 9.

Department of Medical Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China. Electronic address:

Background: A large number of breast cancer survivors suffer from psychological distress. The purpose of this study was to investigate the association between genetic variations in Chinese breast cancer patients and anxiety or depression, and to screen patients who are susceptible to psychological problems.

Methods: A total of 300 early-stage breast cancer patients were recruited in this prospective observational single-center cohort study. With reference to the previous literature and the mechanism concerning anxiety and depression, 9 candidate genes and 29 single-nucleotide polymorphisms (SNPs) loci were selected. The association between SNP variations and anxiety/depression were analyzed.

Results: After we incorporated meaningful clinicopathological and demographic factors, multivariate analysis showed that the A/G and G/G genotypes of IFNGR1 (rs2234711) and the T/C and T/T genotypes of BDNF (rs6265) were significantly associated with depression (HR 3.10, P = .008; HR 2.04, P = .03). The G/A and G/G genotypes of IL-10 (rs1554286) remained independent predictors of anxiety (HR 1.85, P = .019).

Conclusions: These findings suggested that variations in IL-10, IFNGR1 and BDNF were associated with anxious/depressive symptoms in early-stage breast cancer patients in China, which could help identify patients at high risk for psychological problems.
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http://dx.doi.org/10.1016/j.clbc.2022.07.002DOI Listing
July 2022

Reduced porin expression with EnvZ-OmpR, PhoPQ, BaeSR two-component system down-regulation in carbapenem resistance of Klebsiella Pneumoniae based on proteomic analysis.

Microb Pathog 2022 Jul 30;170:105686. Epub 2022 Jul 30.

Department of Laboratory Medicine, The Second Xiangya Hospital of Central South University, Changsha, Hunan, China. Electronic address:

Carbapenem-resistant Klebsiella pneumoniae (CRKP) has proven to be an urgent threat to human health. Proteomics (TMT/LC-MS/MS) and bioinformatics approaches were employed to explore the potential mechanisms underlying carbapenem resistance. Proteomic profiling of CRKP and susceptible KP (sKP) isolates revealed the involvement of outer membrane, beta-lactam resistance pathway, and two-component systems (TCSs) in carbapenem resistance. 27 CRKP strains and 27 susceptible Klebsiella pneumoniae strains were isolated from inpatients at the Second Xiangya Hospital, China to verify the mechanisms. Modified carbapenem inactivation method (mCIM) and PCR of common carbapenem resistance genes confirmed that 77.8% (21/27) of CRKP isolates were carbapenemase-producing. Porin decrease in CRKP isolates was found by SDS-PAGE and mRNA levels of major porins (OmpK35 and OmpK36). RT-qPCR detection of two-component systems (envZ, ompR, phoP, phoQ, baeS and baeR) revealed down-regulation of EnvZ-OmpR, PhoPQ, BaeSR TCSs. Expression of the TCSs, except ompR, were closely correlated with OMPs with the R-value >0.7. Together, this study reaffirmed the significance of the β-lactam resistance pathway in CRKP based on proteomic analysis. OmpK35/36 porin reduction and the controversial downregulation of EnvZ-OmpR, PhoPQ, and BaeSR TCSs were confirmed in carbapenem resistance of Klebsiella pneumoniae.
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http://dx.doi.org/10.1016/j.micpath.2022.105686DOI Listing
July 2022

Screening differential circular RNAs expression profiles in Vulvar Lichen Sclerosus.

Biomed Eng Online 2022 Aug 1;21(1):51. Epub 2022 Aug 1.

Department of Dermatology, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing, 100730, China.

Background: Vulvar lichen sclerosus (VLS) is one of the most common clinical manifestations of vulva. Thirteen percent of women have symptomatic vulvar diseases. The aim of this study is to investigate the expression profile of circular RNA (circRNAs) in vulvar lichen sclerosus, and to identify the underlying core genes of VLS.

Methods: We removed rRNA for sequencing, and screened the differentially expressed messenger RNA (mRNAs), long non-coding RNA (lncRNAs) and single-stranded circRNA in 20 groups of VLS tissues and 20 groups of healthy female vulvar skin tissues. Bioinformatics analysis was used to analyze its potential functions.

Results: A total of 2545 differentially expressed mRNAs were assessed in VLS patients, of which 1541 samples were up-regulated and 1004 samples were down-regulated. A total of 1453 differentially expressed lncRNAs were assessed, of which 812 samples were up-regulated and 641 samples were down-regulated. A total of 79 differentially expressed circRNAs were assessed, of which 54 were up-regulated and 25 were down-regulated. The differential expression of circRNAs was closely related to biological processes and molecular functions. The differences in circRNAs were mainly related to the "human T-cell leukemia virus 1 infection" signaling pathway and the "axon guidance" signaling pathway.

Conclusion: The profile of abnormal regulation of circRNA exists in VLS. According to biological informatics analysis, the dysregulation of circRNAs may be related to the pathogenesis and pathological process of VLS.
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http://dx.doi.org/10.1186/s12938-022-01013-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9344620PMC
August 2022

Recent Progresses in Electrochemical DNA Biosensors for SARS-CoV-2 Detection.

Front Bioeng Biotechnol 2022 15;10:952510. Epub 2022 Jul 15.

Key Laboratory of Prevention and Treatment of Cardiovascular and Cerebrovascular Diseases, Ministry of Education, Key Laboratory of Biomaterials and Biofabrication in Tissue Engineering of Jiangxi Province, Key Laboratory of Biomedical Sensors of Ganzhou, School of Public Health and Health Management, School of Medical and Information Engineering, Gannan Medical University, Ganzhou, China.

Coronavirus disease 19 (COVID-19) is still a major public health concern in many nations today. COVID-19 transmission is now controlled mostly through early discovery, isolation, and therapy. Because of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the contributing factor to COVID-19, establishing timely, sensitive, accurate, simple, and budget detection technologies for the SARS-CoV-2 is urgent for epidemic prevention. Recently, several electrochemical DNA biosensors have been developed for the rapid monitoring and detection of SARS-CoV-2. This mini-review examines the latest improvements in the detection of SARS-COV-2 utilizing electrochemical DNA biosensors. Meanwhile, this mini-review summarizes the problems faced by the existing assays and puts an outlook on future trends in the development of new assays for SARS-CoV-2, to provide researchers with a borrowing role in the generation of different assays.
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http://dx.doi.org/10.3389/fbioe.2022.952510DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9335408PMC
July 2022

Application of Dynamic F-FDG PET/CT for Distinguishing Intrapulmonary Metastases from Synchronous Multiple Primary Lung Cancer.

Mol Imaging 2022 30;2022:8081299. Epub 2022 Jun 30.

Guangdong Provincial Key Laboratory of Biomedical Imaging, The Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai, Guangdong Province 519000, China.

It has been a big challenge to distinguish synchronous multiple primary lung cancer (sMPLC) from primary lung cancer with intrapulmonary metastases (IPM). We aimed to assess the clinical application of dynamic F-FDG PET/CT in patients with multiple lung cancer nodules. We enrolled patients with multiple pulmonary nodules who had undergone dynamic F-FDG PET/CT and divided them into sMPLC and IPM groups based on comprehensive features. The SUV, fitted value based on dynamic scanning, and corresponding maximum diameter ( ) from the two largest tumors were determined in each patient. We determined the absolute between-tumor difference of SUV/ and / (ΔSUV/ ; Δ / ) and assessed the between-group differences. Further, the diagnostic accuracy was evaluated by ROC analysis and the correlation between ΔSUV/ and Δ /D from all groups was determined. There was no significant difference for ΔSUV/ between the IPM and sMPLC groups, while the IPM group had a significantly higher Δ /D than the sMPLC group. The AUC of Δ / for differentiating sMPLC from IPM was 0.80 (cut-off value of = 0.0059, sensitivity 79%, specificity 75%, < 0.001). There was a good correlation (Pearson = 0.91, 95% CI: 0.79-0.96, < 0.0001) between ΔSUV/ and Δ / in the IPM group but not in the sMPLC group (Pearson = 0.45, > 0.05). Dynamic F-FDG PET/CT could be a useful tool for distinguishing sMPLC from IPM. calculation based on Patlak graphic analysis could be more sensitive than SUV in discriminating IPM from sMPLC in patients with multiple lung cancer nodules.
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http://dx.doi.org/10.1155/2022/8081299DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9281433PMC
June 2022

Influence of the Initial Neutrophils to Lymphocytes and Platelets Ratio on the Incidence and Severity of Sepsis-Associated Acute Kidney Injury: A Double Robust Estimation Based on a Large Public Database.

Front Immunol 2022 12;13:925494. Epub 2022 Jul 12.

The 2nd Department of Intensive Care Unit, the Second Affiliated Hospital of Anhui Medical University, Hefei, China.

Background: Acute kidney injury (AKI) is a frequent consequence of sepsis and has been linked to poor prognosis. In critically ill patients, the ratio of neutrophils to lymphocytes and platelets (N/LP) has been confirmed as an inflammation-related marker connected with the development of renal dysfunction. However, the effect of the N/LP ratio on the initiation and development of AKI in patients with sepsis remained unclear. The purpose of this study was to determine if the N/LP ratio on intensive care unit (ICU) admission was associated with the occurrence of sepsis-associated AKI (S-AKI) and severe AKI.

Methods: Adult septic patients from the Medical Information Mart for Intensive Care-IV database were screened and classified into three categories (low, middle, or high) based on their N/LP ratio quartiles. The Cox proportional hazard and competing risk models were used to determine the risk of S-AKI in various N/LP groups, whilst the logistic regression model and restricted cubic splines (RCS) analysis were employed to investigate the link between N/LP ratios and the occurrence of severe AKI. Finally, we did a doubly robust estimation, a subgroup analysis, and a sensitivity analysis to determine the findings' robustness.

Results: We categorized 485, 968, and 485 septic patients into three groups based on their N/LP ratios: low, intermediate, and high. According the Cox proportional hazard model, the hazard rate (95% CI) for those in the middle and high N/LP groups on the incidence of S-AKI were 1.30(1.07, 1.58) and 1.27(1.02, 1.59), respectively, as compared to those in the low N/LP group. And the Fine-Gray proportional subdistribution hazards model indicated that mortality was not a substantial competing risk for S-AKI. Additionally, multivariate logistic regression revealed that the risk of severe AKI increased 1.83 fold in the high group compared to the low group. The RCS result also suggested that the probability of severe AKI rose significantly when N/LP > 9.5. The consistency of these findings was confirmed using doubly robust estimation. However, subgroup and sensitivity analyses revealed that the association between N/LP and the incidence of S-AKI, severe AKI varied considerably between different populations and diagnostic criteria.

Conclusion: A raised initial N/LP level may induce the development of S-AKI and severe AKI within 7 days after ICU admission in septic patients. These influences were enhanced in elder, male, septic shock, and those with poor health condition. Furthermore, high NLP was more strongly connected to the risk of S-AKI and severe AKI in sepsis patients on the urine output-based AKI criteria than on the serum creatinine-based criteria.
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http://dx.doi.org/10.3389/fimmu.2022.925494DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9320191PMC
August 2022

Feasibility of in vivo CAR T cells tracking using streptavidin-biotin-paired positron emission tomography.

Eur J Nucl Med Mol Imaging 2022 Jul 29. Epub 2022 Jul 29.

NHC Key Laboratory of Nuclear Medicine, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi, Jiangsu, 214063, China.

Background: A novel reporter system, streptavidin (SA)- [ Ga]Ga-labeled biotin ([ Ga]Ga-DOTA-biotin), was constructed and its ability for PET imaging the behaviors of CAR T cells were also evaluated in this study.

Methods: In vitro activity and cytotoxicity of the SA transduced anti-CD19-CAR T (denoted as SA-CD19-CAR T) cells were determined. The feasibility of monitoring proliferation profiles of SA-CD19-CAR T cells using [ Ga]Ga-DOTA-biotin was firstly investigated in a solid tumor model. Also, the pharmacodynamics and pharmacokinetics of the CAR T cells in whole-body hematologic neoplasms were evaluated by bioluminescence imaging and [ Ga]Ga-DOTA-biotin PET imaging simultaneously.

Results: After transduction with SA, the activity and cytotoxicity of the modified CAR T cells were not affected. PET images revealed that the uptakes of [ Ga]Ga-DOTA-biotin in CD19 K562 solid tumors were 0.67 ± 0.32 ID%/g and 1.26 ± 0.13 ID%/g at 30 min and 96 h p.i. after administration of SA-CD19-CAR T cells respectively. It confirmed that the SA-CD19-CAR T cells could effectively inhibit the growth of Raji hematologic tumors. However, low radioactivity related to the proliferation of CD19-CAR T cells was detected in the Raji model.

Conclusion: SA-CD19-CAR T cells were constructed successfully without disturbing the antitumor functions of the cells. The proliferation of the CAR T cells in solid tumors could be early detected by [ Ga]Ga-DOTA-biotin PET imaging.
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http://dx.doi.org/10.1007/s00259-022-05923-5DOI Listing
July 2022

Few-layered BiOI nanosheets enclosed by {1 0-1} facets with oxygen vacancies for highly-efficient removal of water contaminants.

J Hazard Mater 2022 Sep 2;437:129274. Epub 2022 Jun 2.

Key Laboratory of Drinking Water Science and Technology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; Institute of Environmental Research at Greater Bay, Key Laboratory for Water Quality and Conservation of the Pearl River Delta, Ministry of Education, Guangzhou University, Guangzhou 510006, China. Electronic address:

Few-layered BiOI nanosheets (FL-BiOI) were synthesized by intergrowth with BiOCO under room temperature. The photoactivity of FL-BiOI was 2.5 and 9.5 times higher than that of BiOI nanoflakes (NF-BiOI, about 30 nm thickness) and standard visible-light-driven N-TiO, respectively. Moreover, FL-BiOI exhibited a wide pH application range (3.0 - 10.0) and excellent photostability. The characterization results showed FL-BiOI was consisted of 5 - 8 layers with thickness of 4 - 7 nm and enclosed by {1 0 - 1} facets. The ultrathin characteristics could accelerate the charge transfer to the surface due to the shortened transport distance. Compared to NF-BiOI, surface oxygen vacancies and the more negative CB potential were formed on FL-BiOI. The photogenerated electrons were confirmed to be captured by surface oxygen vacancies to effectively reduce surface adsorbed O into HO/O, leaving more h to oxidize organic pollutants. This process was further facilitated by the more negative CB potential of FL-BiOI, resulting in the highly efficient removal of pollutants.
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http://dx.doi.org/10.1016/j.jhazmat.2022.129274DOI Listing
September 2022

ArPNO-catalyzed acylative kinetic resolution of tertiary alcohols: access to 3-hydroxy-3-substituted oxindoles.

Org Biomol Chem 2022 Jul 27. Epub 2022 Jul 27.

NMPA Key Laboratory for Research and Evaluation of Innovative Drug, Key Laboratory of Green Chemical Media and Reactions, Ministry of Education, Collaborative Innovation Center of Henan Province for Green Manufacturing of Fine Chemicals, School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang, Henan 453007, China.

Bifunctional chiral 4-aryl-pyridine--oxides (ArPNO) were reported for the acylative kinetic resolution of 3-hydroxy-3-substituted oxindoles, where the oxygen acts as the nucleophilic site. Using less sterically hindered acetic anhydride, both the recovered tertiary heterocyclic alcohols and the ester products exhibited good to excellent results with -factors up to 167. Control experiments supported the dual activation manner, where the -oxide group and N-H proton in ArPNO were crucial for high selectivity and enhanced catalytic reactivity. Compared with the extensively used chiral NHC, isochalcogenourea, and DMAP catalysts, we found that chiral ArPNO were also efficient organocatalysts in the kinetic resolution of tertiary alcohols.
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http://dx.doi.org/10.1039/d2ob01205gDOI Listing
July 2022

7--Methylluteolin Suppresses the 2,4-Dinitrochlorobenzene-Induced Nrf2/HO-1 Pathway and Atopic Dermatitis-like Lesions.

Antioxidants (Basel) 2022 Jul 8;11(7). Epub 2022 Jul 8.

Department of Pharmacy, College of Pharmacy, Pusan National University, Busan 46241, Korea.

7--methylluteolin (7-ML) is a flavonoid isolated from the aerial parts of (). We describe the anti-atopic dermatitis (AD) effects of 7-ML in tert-butyl hydroperoxide (tBHP)-induced HepG2 cells and 2,4-dinitrochlorobenzene (DNCB)-induced SKH-1 hairless mice. Results demonstrated that 7-ML dose-dependently inhibited the activation of Nrf2 (nuclear factor-erythroid 2-related factor 2) in tBHP-induced HepG2 cells. 7-ML applied topically to our DNCB-induced mouse model upregulated the antioxidant protein expression (phosphorylated Nrf2 (pNrf2), Nrf2, and heme oxygenase-1 (HO-1)) in skin tissues, improved epidermal thickness, and reduced mast cell infiltration into the skin. In addition, 7-ML reduced the serum levels of immunoglobulin E (IgE) and interleukin-4 (IL-4) and improved skin barrier functions. These results suggest that 7-ML should be considered a novel antioxidant and anti-AD agent.
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http://dx.doi.org/10.3390/antiox11071344DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9311504PMC
July 2022

Skin-inspired injectable adhesive gelatin/HA biocomposite hydrogel for hemostasis and full-thickness dermal wound healing.

Biomater Adv 2022 Aug 13;139:212983. Epub 2022 Jun 13.

College of Bioresources Chemical and Materials Engineering, Institute of Biomass & Functional Materials, Shaanxi University of Science &Technology, Xi'an 710021, China. Electronic address:

An insufficient adhesion to wet surfaces and biased functions for therapeutic efficacy are limitations to the application of gelatin and hyaluronic acid. Herein, we developed a simple double-injection approach to prepare a skin-inspired gelatin/HA-based injectable remoistenable adhesive hydrogel (HI/DA-Gel) through a simultaneous crosslinking and bio-compositing strategy of genipin incorporated with dopamine (DA) grafted gelatin and N-hydroxy succinimide (NHS) merged with hyaluronic acid. The integrative crosslinking and bio-compositing strategy led to the formation of a HI/DA-Gel with a highly skin-bionic interconnected internal double network 3D-structure with elevated surface wettability, thermal-stablity, adhesive and mechanical properties as expected. In vitro/in vivo biostudies showed that HI/DA-Gel enhanced collagen deposition, hemostatic effects and upregulated the production of CD31, showing an effective hemostasis and full-thickness dermal wound healing strategy. This work proposes a novel facile double-injection approach for the design of gelatin/ hyaluronic acid multi-functional injectable bio-composite hydrogels for integrated therapeutic effects.
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http://dx.doi.org/10.1016/j.bioadv.2022.212983DOI Listing
August 2022

Eosinophil and Mast Cell Counts in the Stomach and Duodenum of Patients with Functional Dyspepsia without a infection.

Korean J Gastroenterol 2022 07;80(1):28-33

Department of Internal Medicine, Konyang University College of Medicine, Daejeon, Korea.

Background/aims: Symptom-based subtyping of functional dyspepsia (FD) is used to segregate patients into groups with homogenous pathophysiological mechanisms. This study examined whether subtyping could reflect the duodenal and gastric microinflammation in FD patients.

Methods: Twenty-one FD patients without infection were recruited. An endoscopic biopsy was performed in the duodenum 2nd portion, stomach antrum, and body. The eosinophil and mast cell counts per high-power field (×40) were investigated by H&E and c-kit staining, respectively. The degree of inflammatory cell infiltration, atrophy, and intestinal metaplasia was also determined by H&E staining in the stomach. The baseline characteristics and eosinophil and mast cell infiltrations were compared among the three groups (epigastric pain syndrome, postprandial distress syndrome, and overlap).

Results: According to the symptom assessment, seven subjects were classified into the epigastric pain syndrome group, 10 into the postprandial syndrome group, and four into the overlap group. The baseline variables were similar in the three groups. Eosinophil infiltration was more prominent in the duodenum than in the stomach. In contrast, mast cell infiltration was similar in the duodenum and stomach. The eosinophil counts in the duodenum were similar in the three groups. The eosinophil counts in the stomach and mast cell counts in the duodenum and stomach were also similar in the three groups.

Conclusions: Duodenal eosinophil infiltration was prominent in FD patients, but the eosinophil counts were similar regardless of the symptom-based subtypes of FD. Hence, the current symptom-based subtyping of FD does not reflect duodenal eosinophil and mast cell infiltration.
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http://dx.doi.org/10.4166/kjg.2022.036DOI Listing
July 2022

Role of multistage inoculation on the co-composting of food waste and biogas residue.

Bioresour Technol 2022 Jul 22;361:127681. Epub 2022 Jul 22.

School of Energy and Environmental Engineering, University of Science and Technology Beijing, Beijing 100083, China; Beijing Key Laboratory of Resource-oriented Treatment of Industrial Pollutants, Beijing 100083, China. Electronic address:

Effect of diverse Lactobacillus amylophilus, Geobacillus thermoleovorans, and Bacillus subtilis inoculation patterns on the co-composting performance of food waste and biogas residue was explored. Experimental results revealed that, compared to the single-stage inoculation and non-inoculation groups, the multistage inoculation pattern prolonged the thermophilic period during composting, consequently improving organic matter decomposition and humification [with a high germination index (120.9%)]. In addition, it could promote the development of humic substances [with a high humus index (4.3) and biological index (1.4)] and lower emissions of carbon dioxide (CO), methane (CH), and ammonia (NH). Additionally, it could improve the microbial variety and the amounts of functional bacteria (i.e., Chloroflexi) in compost, which might be advantageous for the decomposition of refractory organic materials and plant growth. Therefore, the multistage inoculation pattern is recommended for organic waste composting in terms of its gas emissions, compost quality and efficacy benefits.
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http://dx.doi.org/10.1016/j.biortech.2022.127681DOI Listing
July 2022

Anticoagulants for Acute Ischemic Stroke: Cochrane Review.

Stroke 2022 Aug 25;53(8):e385-e387. Epub 2022 Jul 25.

The George Institute for Global Health, Faculty of Medicine, University of New South Wales, Sydney, Australia (X.W., M.O., L.S., C.S.A.).

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http://dx.doi.org/10.1161/STROKEAHA.121.038015DOI Listing
August 2022

Cyclophosphamide for Treatment of Refractory Chronic Inflammatory Demyelinating Polyradiculoneuropathy: A Systematic Review and Meta-analysis.

Clin Ther 2022 Jul 21. Epub 2022 Jul 21.

Department of Rheumatology and Immunology, Nanchong Central Hospital, The Affiliated Nanchong Central Hospital of North Sichuan Medical College, Nanchong, China; Inflammation and Immunology Key Laboratory of Nanchong City, Sichuan, China. Electronic address:

Purpose: This study evaluates the tolerability and efficacy of cyclophosphamide (CYC) for the treatment of refractory chronic inflammatory demyelinating polyradiculoneuropathy (CIDP).

Methods: We searched PubMed, Embase, Cochrane Library, and 2 Chinese databases (Chinese National Knowledge Infrastructure and SinoMed) for studies published between database inception and September 30, 2021. Articles obtained using the appropriate keywords were independently selected by 2 reviewers on the basis of the established inclusion and exclusion criteria.

Findings: In total, 240 records that were eventually curtailed to 13 studies with 83 patients were retrieved and subsequently included in this evaluation. All 13 studies were included in the systematic review, 7 of which were included in the meta-analysis. The pooled estimate of the response rate was 68% (95% CI, 45%-90%). The pooled estimate of the adverse reaction rate was 8% (95% CI, 0%-15%). The disease duration before the first CYC treatment was negatively correlated with the reduction in the modified Rankin Scale score (r = -0.76, P < 0.001). However, the response rates did not differ significantly between patients of different sexes (P = 0.716) or between patients who received and those who did not receive concurrent glucocorticoids (P = 0.617).

Implications: CYC might be a recommended therapeutic option for patients with refractory CIDP, especially those who are unable to accept rituximab treatment. Earlier CYC treatment yields better therapeutic outcomes in patients with refractory CIDP without CYC-related contraindications.
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http://dx.doi.org/10.1016/j.clinthera.2022.06.008DOI Listing
July 2022

[Analysis of the Characteristics of the Outcomes of Job-Transfer Subspecialty Training Program in Pediatrics in Sichuan].

Sichuan Da Xue Xue Bao Yi Xue Ban 2022 Jul;53(4):597-601

Department of Pediatrics and Key Laboratory of Birth Defects and Related Disease of Women and Children of the Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu 610041, China.

Objective: To discuss the characteristics of physician trainee outcomes after completion of the job-transfer subspecialty training in pediatrics, a program designed to increase the number of pediatricians, in Sichuan Province and to provide countermeasures for alleviating the shortage of pediatricians.

Methods: We collected with questionnaire surveys information on changes in the workload and salaries experienced by physicians who completed the job-transfer subspecialty training program in pediatrics between February 2017 and May 2020 in Sichuan Province. Then, we compared the characteristics of physicians who successful became pediatricians and those who did no.

Results: A total of 208 physicians completed the job-transfer subspecialty training program in pediatrics. Among them, 178, accounting for 85.6%, completed the questionnaire survey, and 120, accounting for 67.4%, had a background in other subspecialties than pediatrics. The majority (>90%) of physicians who participated in the training program came from secondary or lower levels of hospitals from the cities and prefectures all over Sichuan Province. In this study, we found that the rate of successful job transfer from being a physician to being a pediatrician in Sichuan Province in the past four years was 85.0% (102/120), with the year-by-year results being 88.2% (15/17) in 2017, 72.7% (16/22) in 2018, 86.7% (39/45) in 2019, and 94.% (32/34) in 2020. There was no significant difference between physicians who had successful job transfer and became pediatricians and those who failed to do so in terms of gender, age, hospital level, specialization prior to the job transfer, whether or not the hospital had a pediatrics department, amount of support for the pediatrics department, whether or not the physician was working at a new hospital after the job transfer, salaries, and changes of responsibilities during COVID-19 (all >0.05). There was significant difference in the change of workload after completion of the training program between physicians who had successful job transfer and became pediatricians and those who failed to do so ( =9.037, =0.003), and 78.4% of the trainees stated that their workload had increased after the job transfer. There was a moderate correlation between successful job transfer and changes in workload after the transfer (|Phi[ψ] |=0.729).

Conclusions: The policy of government-supported job-transfer subspecialty training in pediatrics has played an active and important role in the swift resolution of the shortage of pediatricians. However, finding the root cause of and addressing the problem of the overwhelming workload of pediatricians remain challenging issues to be resolved.
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http://dx.doi.org/10.12182/20220760301DOI Listing
July 2022

Bioconversion of biowaste into renewable energy and resources: A sustainable strategy.

Environ Res 2022 Jul 19;214(Pt 2):113929. Epub 2022 Jul 19.

School of Energy and Environmental Engineering, University of Science and Technology Beijing, Beijing, 100083, China; Beijing Key Laboratory of Resource-oriented Treatment of Industrial Pollutants, Beijing, 100083, China. Electronic address:

Due to its high amount of organic and biodegradable components that can be recycled, biowaste is not only a major cause of environmental contamination, but also a vast store of useful materials. The transformation of biowaste into energy and resources via biorefinery is an unavoidable trend, which could aid in reducing carbon emissions and alleviating the energy crisis in light of dwindling energy supplies and mounting environmental difficulties related with solid waste. In addition, the current pandemic and the difficult worldwide situation, with their effects on the economic, social, and environmental aspects of human life, have offered an opportunity to promote the transition to greener energy and sources. In this context, the current advancements and possible trends of utilizing widely available biowaste to produce key biofuels (such as biogas and biodiesel) and resources (such as organic acid, biodegradable plastic, protein product, biopesticide, bioflocculant, and compost) are studied in this review. To achieve the goal of circular bioeconomy, it is necessary to turn biowaste into high-value energy and resources utilizing biological processes. In addition, the usage of recycling technologies and the incorporation of bioconversion to enhance process performance are analyzed critically. Lastly, this work seeks to reduce a number of enduring obstacles to the recycling of biowaste for future use in the circular economy. Although it could alleviate the global energy issue, additional study, market analysis, and finance are necessary to commercialize alternative products and promote their future use. Utilization of biowaste should incorporate a comprehensive approach and a methodical style of thinking, which can facilitate product enhancement and decision optimization through multidisciplinary integration and data-driven techniques.
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http://dx.doi.org/10.1016/j.envres.2022.113929DOI Listing
July 2022

Gut Microbiota and Transcriptomics Reveal the Effect of Human Norovirus Bioaccumulation on Oysters (Crassostrea gigas).

Microbiol Spectr 2022 Jul 5:e0016122. Epub 2022 Jul 5.

Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Laboratory for Marine Drugs and Bioproducts of Pilot National Laboratory for Marine Science and Technology, Qingdao, China.

Human norovirus (HuNoV) is a major foodborne pathogen that causes acute viral gastroenteritis, and oysters are one of the main carriers of HuNoV transmission. While progress has been made toward understanding the pattern of oyster-bioaccumulated HuNoV, the response of oysters to HuNoV bioaccumulation, including changes in gene expression and gut microbiota, is unclear. In this study, histo-blood group antigen (HBGA)-like molecule expression and gene regulation features and the HuNoV-microbiome interactions of oysters during HuNoV bioaccumulation were characterized. With the prolongation of bioaccumulation time, the HuNoV content and expression of type A HBGA-like molecules in oysters increased and stabilized. HuNoV also altered the expression of immunity- and glycosphingolipid biosynthesis-related genes. Prolonged bioaccumulation of HuNoV can reduce the abundance and change the composition of the oyster gut microbiota. In particular, with the extension of bioaccumulation time, the abundance of , , , , , , and decreased, while the abundance of and increased. This study provides potential candidates for identifying functional genes involved in the bioaccumulation of HuNoV in oysters. More importantly, it provides the first description of the changes in gut microbiota during HuNoV bioaccumulation in oysters. The role of the oyster gut microbiota in HuNoV bioaccumulation is poorly understood. This study revealed, for the first time, the changes in gut microbiota and gene expression of oysters with HuNoV bioaccumulation. This study enriches the understanding of the impact of HuNoV bioaccumulation on oysters and provides a new direction for the study of the molecular mechanism of HuNoV bioaccumulation in oysters.
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http://dx.doi.org/10.1128/spectrum.00161-22DOI Listing
July 2022

Poloxamer 407 and Hyaluronic Acid Thermosensitive Hydrogel-Encapsulated Ginsenoside Rg3 to Promote Skin Wound Healing.

Front Bioeng Biotechnol 2022 5;10:831007. Epub 2022 Jul 5.

College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, China.

Ginsenoside Rg3 has shown beneficial effects in various skin diseases. The current interest in designing and developing hydrogels for biomedical applications continues to grow, inspiring the further development of drug-loaded hydrogels for tissue repair and localized drug delivery. The aim of the present study was to develop an effective and safe hydrogel (Rg3-Gel), using ginsenoside Rg3, and we evaluated the wound-healing potential and therapeutic mechanism of Rg3-Gel. The results indicated that the optimized Rg3-Gel underwent discontinuous phase transition at low and high temperatures. Rg3-Gel also exhibited good network structures, swelling water retention capacity, sustainable release performance, and excellent biocompatibility. Subsequently, the good antibacterial and antioxidant properties of Rg3-Gel were confirmed by tests. In full-thickness skin defect wounded models, Rg3-Gel significantly accelerated the wound contraction, promoted epithelial and tissue regeneration, and promoted collagen deposition and angiogenesis. In addition, Rg3-Gel increased the expression of autophagy proteins by inhibiting the MAPK and NF-KB pathways . It simultaneously regulated host immunity by increasing the abundance of beneficial bacteria and the diversity of the wound surface flora. From these preliminary evaluations, it is possible to conclude that Rg3-Gel has excellent application potential in wound-healing drug delivery systems.
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http://dx.doi.org/10.3389/fbioe.2022.831007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9294355PMC
July 2022

Evaluating the Microsatellite Instability of Colorectal Cancer Based on Multimodal Deep Learning Integrating Histopathological and Molecular Data.

Front Oncol 2022 5;12:925079. Epub 2022 Jul 5.

Science System Department, Geneis Beijing Co., Ltd., Beijing, China.

Microsatellite instability (MSI), an important biomarker for immunotherapy and the diagnosis of Lynch syndrome, refers to the change of microsatellite (MS) sequence length caused by insertion or deletion during DNA replication. However, traditional wet-lab experiment-based MSI detection is time-consuming and relies on experimental conditions. In addition, a comprehensive study on the associations between MSI status and various molecules like mRNA and miRNA has not been performed. In this study, we first studied the association between MSI status and several molecules including mRNA, miRNA, lncRNA, DNA methylation, and copy number variation (CNV) using colorectal cancer data from The Cancer Genome Atlas (TCGA). Then, we developed a novel deep learning framework to predict MSI status based solely on hematoxylin and eosin (H&E) staining images, and combined the H&E image with the above-mentioned molecules by multimodal compact bilinear pooling. Our results showed that there were significant differences in mRNA, miRNA, and lncRNA between the high microsatellite instability (MSI-H) patient group and the low microsatellite instability or microsatellite stability (MSI-L/MSS) patient group. By using the H&E image alone, one can predict MSI status with an acceptable prediction area under the curve (AUC) of 0.809 in 5-fold cross-validation. The fusion models integrating H&E image with a single type of molecule have higher prediction accuracies than that using H&E image alone, with the highest AUC of 0.952 achieved when combining H&E image with DNA methylation data. However, prediction accuracy will decrease when combining H&E image with all types of molecular data. In conclusion, combining H&E image with deep learning can predict the MSI status of colorectal cancer, the accuracy of which can further be improved by integrating appropriate molecular data. This study may have clinical significance in practice.
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http://dx.doi.org/10.3389/fonc.2022.925079DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9295995PMC
July 2022

A new insight into the influencing factors of natural attenuation of chlorinated hydrocarbons contaminated groundwater: A long-term field study of a retired pesticide site.

J Hazard Mater 2022 Jul 13;439:129595. Epub 2022 Jul 13.

Nanjing Institute of Environmental Science, Ministry of Ecology and Environment, Nanjing 210042, China; Key Laboratory of Soil Environmental Management and Pollution Control, Ministry of Ecology and Environment, Nanjing 210042, China. Electronic address:

Natural attenuation of contaminants has been increasingly applied as a strategy to manage the retired pesticide manufacturing sites due to the increasing restrictions on the reuse of contaminated sites in China. However, the influencing factors to enhance natural attenuation for chlorinated hydrocarbons in retired pesticide sites were not well studied. In this paper, monitoring of pollutants, environmental factors and microbial community was conducted from 2016 to 2021 in a retired pesticide site in Jiangsu Province undergoing natural attenuation, where the groundwater was severely contaminated with chlorinated hydrocarbons. The spatial variation of main pollutants, including chlorinated ethenes and ethanes, indicated that the site could be divided into the source area, diffusion area, and the end of diffusion area, where organohalide-respiring bacteria (OHRB) were detected. Pollutants and environmental factors influenced the OHRB community structure, which explained 7.6% and 33.2% of the variation, respectively. The abundances of obligate and facultative OHRB were affected in opposite ways by pollutants and environmental factors. Dehalococcoides and Dehalogenimonas in obligate OHRB were significantly inhibited by sulfate (r = -0.448, p < 0.05). The spatial-temporal characteristics of pollutants and the reveal of microbial community structure and its restricting factors in different areas make the foundation for strengthening the implementation of natural attenuation.
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http://dx.doi.org/10.1016/j.jhazmat.2022.129595DOI Listing
July 2022
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