Publications by authors named "Min Yang"

2,663 Publications

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[Effects of noise, bright light and mechanical stimulation on sleep, blood-brain barrier and cognitive function in septic rats].

Zhonghua Wei Zhong Bing Ji Jiu Yi Xue 2021 May;33(5):529-534

Department of Critical Care Medicine, Xiaogan Hospital Affiliated to Wuhan University of Science and Technology (Xiaogan Central Hospital), Xiaogan 432000, Hubei, China. Corresponding author: Yao Peng, Email:

Objective: To evaluate the effects of noise, bright light and mechanical stimulation on sleep, blood-brain barrier and cognitive function in septic rats.

Methods: Forty male Sprague-Dawley (SD) rats were selected and intraperitoneal injection of 10 mg/kg lipopolysaccharide (LPS) was used to establish sepsis model. 0, 30, 45, 60, 75 dB noise stimulation or 0, 50, 100, 200, 400 Lux light stimulation were given to rats (all n = 4). The serum levels of cortisol and melatonin, and the cerebral content of Evans blue (EB) were measured 96 hours after the stimulation to determine the optimal intensity of intervention. The other 40 SD rats were randomly divided into control group (Con group), LPS group, noise intervention group (LPS+60 dB group), 200 Lux light intervention group (LPS+200 Lux group) and mechanical stimulation group (LPS+MS group), with 8 rats in each group. The open fields test and fear conditioning test were used to evaluate the exploratory behavior and cognitive function 96 hours after corresponding stimulation. The enzyme linked immunosorbent assay (ELISA) was used to detect cerebral level of interleukin-6 (IL-6) and serum levels of cortisol and melatonin. The blood-brain barrier integrity was assessed by EB staining. The protein levels of ZO-1, Claudin-5 and caspase-3 in the hippocampus were detected by Western blotting to assess the blood-brain barrier integrity and neuronal apoptosis.

Results: Compared with 0 dB group or 0 Lux group, the serum melatonin concentration in 60 dB group and 200 Lux group were significantly reduced, while the serum cortisol concentration and cerebral EB content were significantly increased. Therefore, 60 dB noise and 200 Lux light were selected in the subsequent experiments. Compared with Con group, the horizontal score and vertical score in the open field test in LPS group were significantly decreased. There were no significant differences in the proportion of freezing time, the cerebral contents of EB and IL-6, the serum levels of melatonin and cortisol, and the hippocampal expressions of ZO-1, Claudin-5 and caspase-3. Compared with LPS group, the horizontal score, vertical score and the percentage of freezing time in LPS+60 dB group, LPS+200 Lux group and LPS+MS group were significantly reduced [horizontal score: 73.8±9.7, 80.3±9.4, 64.5±8.3 vs. 103.6±15.5; vertical score: 9.4±1.7, 11.2±1.9, 6.8±0.9 vs. 15.9±2.8; the percentage of freezing time: (45.3±4.7)%, (53.3±5.8)%, (42.1±5.1)% vs. (66.1±6.3)%], the serum level of melatonin was significantly decreased (ng/L: 53.62±6.20, 44.25±6.41, 45.33±5.84 vs. 74.39±7.54), the serum level of cortisol was significantly increased (nmol/L: 818.34±95.53, 710.04±65.41, 989.73±91.63 vs. 398.82±72.59), the levels of EB, IL-6 in the brain tissue were significantly increased [EB (μg/g): 2.80±0.35, 2.38±0.31, 3.24±0.42 vs. 1.59±0.26; IL-6 (ng/g): 31.56±4.11, 26.69±3.75, 37.47±4.56 vs. 16.28±2.69], the expressions of ZO-1 and Claudin-5 were significantly decreased (ZO-1/β-actin: 0.37±0.04, 0.32±0.05, 0.24±0.04 vs. 0.80±0.09; Claudin-5/β-actin: 0.62±0.08, 0.47±0.06, 0.35±0.05 vs. 0.97±0.20), and the expression of cleaved caspase-3 was significantly increased (caspase-3/β-actin: 0.56±0.06, 0.39±0.04, 0.72±0.12 vs. 0.20±0.03), with statistically significant differences (all P < 0.05).

Conclusions: 60 dB noise, 200 Lux light or mechanical stimulation for 96 hours could inhibit the secretion of serum melatonin, promote the secretion of cortisol, and activate neuroinflammation in septic rats, and lead to neuronal apoptosis in hippocampus and hyper-permeability of blood-brain barrier, which in turn could cause sleep disturbance and cognitive impairment.
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http://dx.doi.org/10.3760/cma.j.cn121430-20200630-00514DOI Listing
May 2021

Selective separation of main flavonoids by combinational use of ionic liquid-loaded microcapsules from crude extract of Tartary buckwheat.

Food Chem 2021 May 30;362:130255. Epub 2021 May 30.

School of Chemical Engineering, Sichuan University, Chengdu 610065, PR China. Electronic address:

For selective adsorption of main flavonoids from crude Tartary buckwheat extract (rutin, 0.021 mg/mL; quercetin, 0.030 mg/mL; and kaempferol, 0.011 mg/mL), new ionic liquid-based sorbents were successfully prepared by encapsulating [Bmim]Br and [Bmim]Pro in regular spherical non-magnetic and magnetic microcapsules with polysulfone content of 8%, respectively. After appropriate loading process, the microcapsules were comprehensively characterized by infrared spectroscopy, thermogravimetry analysis and scanning electron microscopy. Then the separation strategy was designed to separate rutin and quercetin from kaempferol by combinational use of two kinds of IL-loaded microcapsules (ILLMs). The effects of solid-liquid ratio of ILLMs and extract, pH, time and adsorption temperature were all investigated. The experimental data fit well with the quasi-second-order kinetics model and Langmuir model. After desorption, target flavonoids were well recovered and the ILLMs showed good stability. As the result, a new IL-based separation technology for main flavonoids from food crop was developed for the first time.
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http://dx.doi.org/10.1016/j.foodchem.2021.130255DOI Listing
May 2021

A systematic review of myasthenia gravis complicated with myocarditis.

Brain Behav 2021 Jun 9. Epub 2021 Jun 9.

Department of Respiratory and Critical Care Medicine, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China.

Among many of the autoimmune diseases observed in patients with myasthenia gravis (MG), myocarditis is one of the most critical. The goal of this review is to systematically describe and investigate the characteristics of MG complicated with myocarditis. We identified 183 records in PubMed (MEDLINE), Web of Science, and EMBASE from 1948 to September 10, 2020. Studies were included if they presented clinical data on MG complicated with myocarditis. Of the 35 patients from 28 studies in this review, 57.14% (20/35) were males, with a mean age of 59.11 ± 15.87. Dyspnea was the most common cardiac symptom accounting for over 60% in the study. Among the 35 patients, 13 cases of myocarditis occurred concomitantly with MG and the longest interval between MG and myocarditis was 7 years. Forty percent of patients developed myocarditis caused by immune checkpoint inhibitors (ICI). Among the patients with myocarditis, over half of the patients were diagnosed by myocardial biopsy. After active immune regulation and symptomatic treatment, only 15 of 35 patients with MG complicated with myocarditis improved, 18 patients died during hospitalization, one patient died due to tumor progression and 1patient died 5 years later. The prognosis of patients with MG complicated with myocarditis is poor, and myocardial enzymes and other indexes need to be monitored for patients taking ICI drugs. Patients with dyspnea who are still not ideally treated by mechanical ventilation should be vigilant against the occurrence of MG complicated with myocarditis.
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http://dx.doi.org/10.1002/brb3.2242DOI Listing
June 2021

Predicting Risk of Pulmonary Arterial Hypertension in Systemic Lupus Erythematosus: CSTAR Cohort Study.

Arthritis Rheumatol 2021 Jun 9. Epub 2021 Jun 9.

Department of Rheumatology and Clinical Immunology, Chinese Academy of Medical Sciences & Peking Union Medical College, National Clinical Research Center for Dermatologic and Immunologic Diseases (NCRC-DID), Ministry of Science & Technology, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital (PUMCH), Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education, Beijing, China.

Objectives: Pulmonary arterial hypertension is a life-threatening complication of systemic lupus erythematosus. However, there is no algorithm to identify those at high risk. We aimed to develop a prediction model for pulmonary arterial hypertension in lupus patients that provides individualized risk estimates.

Methods: A multicenter, longitudinal cohort study was undertaken from January 2003 to January 2020. The study collected data on 3,624 consecutively evaluated patients diagnosed with lupus. The diagnosis of pulmonary arterial hypertension was confirmed by right heart catheterization. Cox proportional hazards regression and least absolute shrinkage and selection operator were used to fit the model. Model discrimination, calibration, and decision curve analysis were assessed for validation.

Results: Ninety-two lupus patients developed pulmonary arterial hypertension (2.54%) at a median follow-up of 4.84 years (interquartile range, 2.42-8.84). The final prediction model included five clinical variables (acute/subacute cutaneous lupus, arthritis, renal disorder, thrombocytopenia, and interstitial lung disease) and three autoantibodies (anti-RNP, anti-Ro/SSA and anti-La/SSB). A 10-year pulmonary arterial hypertension probability-predictive nomogram was established. The model was internally validated by C statistic (0.78), the Brier score (0.03), and a satisfactory calibration curve. According to the net benefit and predicted probability thresholds, we recommend annual screening in high-risk (> 4.62 %) lupus patients.

Conclusion: We developed a risk stratification model using routine clinical assessments. This new tool may effectively predict the future risk of pulmonary arterial hypertension in patients with systemic lupus erythematosus.
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http://dx.doi.org/10.1002/art.41740DOI Listing
June 2021

Rapid controlled synthesis of gold-platinum nanorods with excellent photothermal properties under 808 nm excitation.

Beilstein J Nanotechnol 2021 17;12:462-472. Epub 2021 May 17.

MicroNano System Research Center, College of Information and Computer & Key Laboratory of Advanced Transducers and Intelligent Control System of Ministry of Education and Shanxi Province, Taiyuan University of Technology, Taiyuan 030024, Shanxi, China.

Noble metal nanomaterials are particularly suitable as photothermal transduction agents (PTAs) with high photothermal conversion efficiency (PCE) due to local surface plasmon resonance (LSPR). Studies on different gold-platinum (Au-Pt) bimetal nanoparticles exhibiting the LSPR effect have provided a new idea for the synthesis of excellent PTAs. But there is no simple and scalable method for the controllable synthesis of Au-Pt nanoparticles with adjustable LSPR wavelength range. In this work, the effects of Ag and KPtCl on the deposition of Pt on the surface of gold nanorods (AuNRs) were investigated. A fast, precise, and controlled synthesis of dumbbell-like Pt-coated AuNRs ([email protected] NRs) under mild conditions is proposed. The synthesized [email protected] NRs have a longitudinal LSPR wavelength of 812 nm, which is very close to a common laser wavelength of 808 nm. The [email protected] NRs exhibit excellent photothermal properties when irradiated with a laser. The temperature increased by more than 36 °C after irradiation for 10 min, with a PCE of about 78.77%, which is much higher than that of AuNRs (57.33%). In addition, even after four on/off cycles, the [email protected] NRs are able to maintain the photothermal properties and retain their optical properties, indicating that they have excellent photothermal stability and reusability.
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http://dx.doi.org/10.3762/bjnano.12.37DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8144918PMC
May 2021

Suspect and target screening of emerging pesticides and their transformation products in an urban river using LC-QTOF-MS.

Sci Total Environ 2021 May 24;790:147978. Epub 2021 May 24.

Key Laboratory of Drinking Water Science and Technology, Research Center for Eco-Environmental Sciences, University of Chinese Academy of Sciences, Beijing 100085, China; Sino-Danish Center for Education and Research, University of Chinese Academy of Sciences, Beijing 100190, China. Electronic address:

This study sheds light on the occurrence of emerging pesticides and their transformation products (TPs) in an urban river in Beijing that is mainly supplemented with treated wastewater. To this end, suspect and non-target screening was conducted using a database of 557 commercial pesticides and over 1400 predicted TPs. Finally, 30 pesticides and 20 TPs were identified, with 12 pesticides and 10 TPs detected in all samples. Eleven pesticides and 17 TPs were detected in Beijing for the first time. Among these, 18 compounds were confirmed using authentic standards. Concentrations of the confirmed and suspected compounds were determined by quantification and semi-quantification, respectively, based on 18 authentic standards. Fungicides and their TPs constituted the largest group and exhibited the highest total concentration (26 compounds; 52.2 μg/L), followed by insecticides (14 compounds; 51.3 μg/L) and herbicides (10 compounds; 24.5 μg/L). DEET, carbendazim, prometryn, ω-carboxylic acid, 2-aminobenzimidazole, metolachlor TP, hexaconazole TP, metalaxyl TP, and azoxystrobin TP exhibited relatively high mean concentration (>100 ng/L). Among the 20 TPs, approximately 65% showed higher concentrations than their parent compounds. Correlation analysis revealed that 6 pesticides and 10 TPs in the river were mainly contributed by the discharge from a wastewater treatment plant. Although a majority of the emerging pesticides had low toxicity, 10 pesticides exhibited high risks to aquatic systems, especially invertebrates.
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http://dx.doi.org/10.1016/j.scitotenv.2021.147978DOI Listing
May 2021

circ0101675 promotes malignant process via sponging miR-1278 and upregulating WNT3A/5A in non-small cell lung cancer.

J Cancer 2021 17;12(14):4209-4217. Epub 2021 May 17.

Key Laboratory of Cancer Cellular and Molecular Pathology in Hunan Province, Cancer Research Institute, University of South China, Hengyang 421001, Hunan Province, China.

Circular RNAs (circRNAs) is one type of non-coding RNAs (ncRNAs) which have many roles in biological processes, as well as modulation intracellular gene expression modulation. Nonethless, the roles along with expression status of the most circRNAs in NSCLC (non-small cell lung cancer) remain unknown. Herein, we conducted a high-throughput microarray sequencing to identify abnormal expressed circRNAs. Circ0101675 was found upregulated in NSCLC cell lines and tissues. We carried out colony formation, transwell, CCK-8, and animal assays to investigate the functions of circ0101675. Silence of circ0101675 inhibited the migration and proliferation of NSCLC. To elucidate the mechanism, RNA immunoprecipitation assays along with luciferase enzyme reporter assays were further employed to explore the cross-talk between circ0101675 and other molecules. We discovered that circ0101675 facilitates the malignant process of growth and migration via sponging miR-1278 and upregulating WNT3A/5A expression. In conclusion, we revelaed the vital role of circ0101675-miR-1278-WNT3A/5A signaling in NSCLC progression via the competing endogenous RNAs mechanism. Therefore, circ0101675 can be used as a new and useful biomarker for monitoring and treating NSCLC.
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http://dx.doi.org/10.7150/jca.57255DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8176403PMC
May 2021

Assessment of complications and short-term outcomes of percutaneous peritoneal dialysis catheter insertion by conventional or modified Seldinger technique.

Ren Fail 2021 Dec;43(1):919-925

Department of Nephrology, The Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu Province, China.

Objective: To explore the efficacy and short-term complications of a modified technique to percutaneously insert a peritoneal dialysis catheter.

Methods: We reviewed the outcomes of 94 patients who underwent peritoneal dialysis catheterization between October 2017 and April 2020. Of these, 47 cases were placed by a conventional Seldinger technique, whereas 47 cases were placed by a modified technique based on the Seldinger method. The success rates of the catheter insertion and three-month postoperative complications were compared between these two groups.

Results: The catheter insertion success rates were comparable between the two groups: 93.6% in the conventional technique group and 97.9% in the modified technique group ( = 0.307). The incidence of postoperative catheter migration was lower using the modified technique (4.3%) than the conventional technique (18.3%) ( = 0.037). None of the patients in the modified technique group had postoperative dialysate leakage, whereas this occurred in 9.0% of patients in the conventional technique group ( = 0.036). There were no statistically significant differences in the incidence of postoperative bleeding, infection, or visceral damage between the two groups.

Conclusions: The modified Seldinger technique for percutaneous peritoneal dialysis catheter insertion reduced the short-term postoperative complications of catheter migration and dialysate leakage, with a comparable successful catheter insertion rate compared with the conventional Seldinger technique.
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http://dx.doi.org/10.1080/0886022X.2021.1925296DOI Listing
December 2021

S(vi) in three-component sulfonamide synthesis: use of sulfuric chloride as a linchpin in palladium-catalyzed Suzuki-Miyaura coupling.

Chem Sci 2021 Apr 6;12(18):6437-6441. Epub 2021 Apr 6.

School of Pharmaceutical and Materials Engineering, Institute for Advanced Studies, Taizhou University 1139 Shifu Avenue Taizhou 318000 China

Sulfuric chloride is used as the source of the -SO- group in a palladium-catalyzed three-component synthesis of sulfonamides. Suzuki-Miyaura coupling between the generated sulfamoyl chlorides and boronic acids gives rise to diverse sulfonamides in moderate to high yields with excellent reaction selectivity. Although this transformation is not workable for primary amines or anilines, the results show high functional group tolerance. With the solving of the desulfonylation problem and utilization of cheap and easily accessible sulfuric chloride as the source of sulfur dioxide, redox-neutral three-component synthesis of sulfonamides is first achieved.
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http://dx.doi.org/10.1039/d1sc01351cDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8115270PMC
April 2021

Immune-related genes and gene sets for predicting the response to anti-programmed death 1 therapy in patients with primary or metastatic non-small cell lung cancer.

Oncol Lett 2021 Jul 19;22(1):540. Epub 2021 May 19.

Thoracic Medicine Department 2, Cancer Hospital Affiliated to Xiangya Medical College, Central South University, Changsha, Hunan 410013, P.R. China.

Although antibodies targeting the immune checkpoint protein programmed death-1 (PD-1) exert therapeutic effects in patients with primary or metastatic non-small cell lung cancer (NSCLC), the majority of patients exhibit partial or complete resistance to anti-PD1 treatment. Thus, the aim of the present study was to identify reliable biomarkers for predicting the response to anti-PD-1 therapy. The present study analyzed tumor specimens isolated from 24 patients (13 with primary and 11 with metastatic NSCLC) prior to treatment with approved PD1-targeting antibodies. The expression profile of 395 immune-related genes was examined using RNA immune-oncology panel sequencing. The results demonstrated that six immune-related differently expressed genes (DEGs), including HLA-F-AS1, NCF1, RORC, DMBT1, KLRF1 and IL-18, and five DEGs, including HLA-A, HLA-DPA1, TNFSF18, IFI6 and PTK7, may be used as single biomarkers for predicting the efficacy of anti-PD-1 treatment in patients with primary and with metastatic NSCLC, respectively. In addition, two DEG sets comprising either six (HLA-F-AS1, NCF1, RORC, DMBT1, KLRF and IL-18) or two (HLA-A and TNFSF18) DEGs as potential combination biomarkers for predicting the efficacy of anti-PD-1 therapy in patients with NSCLC. Patients with a calculated expression level of the DEG sets >6.501 (primary NSCLC) or >6.741 (metastatic NSCLC) may benefit from the anti-PD-1 therapy. Overall, these findings provided a basis for the identification of additional biomarkers for predicting the response to anti-PD-1 treatment.
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http://dx.doi.org/10.3892/ol.2021.12801DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8161458PMC
July 2021

Haplopine Ameliorates 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis-Like Skin Lesions in Mice and TNF-α/IFN-γ-Induced Inflammation in Human Keratinocyte.

Antioxidants (Basel) 2021 May 19;10(5). Epub 2021 May 19.

College of Pharmacy, Pusan National University, Busan 46241, Korea.

This study aimed to investigate the anti-inflammatory, antioxidant, and anti-atopic dermatitis (AD) effects of haplopine, which is one of the active components in Haplopine (12.5 and 25 μM) inhibited the mRNA expressions of inflammatory cytokines IL-6, TSLP, GM-CSF, and G-CSF and the protein expressions of IL-6 and GM-CSF in TNF-α/INF-γ-stimulated HaCaT cells. In HO-induced Jukat T cells, haplopine (25 and 50 μM) suppressed the productions of proinflammatory cytokines (IL-4, IL-13, and COX-2) and increased the mRNA and protein expressions of oxidative stress defense enzymes (SOD, CAT, and HO-1) in a concentration-dependent manner. In vivo, haplopine significantly attenuated the development of AD symptoms in 2,4-dinitrochlorobenzene (DNCB)-stimulated Balb/c mice, as evidenced by reduced clinical dermatitis scores, skin thickness measurements, mast cell infiltration, and serum IgE concentrations. These findings demonstrate that haplopine should be considered a novel anti-atopic agent with the potential to treat AD.
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http://dx.doi.org/10.3390/antiox10050806DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8161082PMC
May 2021

Energetic-Materials-Driven Synthesis of Graphene-Encapsulated Tin Oxide Nanoparticles for Sodium-Ion Batteries.

Materials (Basel) 2021 May 14;14(10). Epub 2021 May 14.

School of Materials Science and Engineering, Beijing Institute of Technology, Beijing 100081, China.

By evenly mixing polytetrafluoroethylene-silicon energetic materials (PTFE-Si EMs) with tin oxide (SnO) particles, we demonstrate a direct synthesis of graphene-encapsulated SnO (Gr-SnO) nanoparticles through the self-propagated exothermic reaction of the EMs. The highly exothermic reaction of the PTFE-Si EMs released a huge amount of heat that induced an instantaneous temperature rise at the reaction zone, and the rapid expansion of the gaseous SiF product provided a high-speed gas flow for dispersing the molten particles into finer nanoscale particles. Furthermore, the reaction of the PTFE-NPs with Si resulted in a simultaneous synthesis of graphene that encapsulated the SnO nanoparticles in order to form the core-shell nanostructure. As sodium storage material, the graphene-encapsulated SnO nanoparticles exhibit a good cycling performance, superior rate capability, and a high initial Coulombic efficiency of 85.3%. This proves the effectiveness of our approach for the scalable synthesis of core-shell-structured graphene-encapsulated nanomaterials.
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http://dx.doi.org/10.3390/ma14102550DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8157060PMC
May 2021

Outcomes and Loop Pattern Analysis of a Road-Map Technique for ERCP with Side-Viewing Duodenoscope in Patients with Billroth II Gastrectomy (with Video).

J Pers Med 2021 May 12;11(5). Epub 2021 May 12.

Department of Gastroenterology, Ajou University School of Medicine, 164 Worldcup-ro, Yeongtong-gu, Suwon 16499, Korea.

Endoscopic retrograde cholangiopancreatography (ERCP) in patients who have undergone a Billroth II gastrectomy is a major challenge. This study aimed to evaluate the outcomes of the road-map technique for duodenal intubation using a side-viewing duodenoscope for ERCP in Billroth II gastrectomy patients with naïve papilla, and to analyze the formation and release patterns of common bowel loops that occur when the duodenoscope navigates the afferent limb. The duodenoscopy approach success rate was 85.8% (97/113). In successful duodenoscopy approach patients, there were five bowel looping patterns that occurred when the preceding catheter-connected duodenoscope was advanced into the duodenum: (1) reverse ɣ-loop (29.9%), (2) fixed reverse ɣ-loop (5.2%), (3) simple U-loop (22.7%), (4) N-loop (28.9%), and (5) reverse alpha loop (13.4%). The duodenoscopy cannulation and duodenoscopy therapeutic success rates were 81.4% (92/113) and 80.5% (91/113), respectively, while the overall cannulation and therapeutic success rates were 92.0% (104/113) and 87.6% (99/113), respectively. Bowel perforation occurred in three patients (2.7%). The road-map technique may benefit duodenoscope-based ERCP in Billroth II gastrectomy patients by minimizing the tangential axis alignment between the duodenoscopic tip and driving of the afferent limb, and by predicting and counteracting bowel loops that occur when the duodenoscope navigates the afferent limb.
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http://dx.doi.org/10.3390/jpm11050404DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8150314PMC
May 2021

Occurrence, fates, and carcinogenic risks of substituted polycyclic aromatic hydrocarbons in two coking wastewater treatment systems.

Sci Total Environ 2021 May 17;789:147808. Epub 2021 May 17.

National Engineering Laboratory for Industrial Wastewater Treatment, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

This paper reports for the first time the occurrence, fates, and carcinogenic risks of 20 substituted polycyclic aromatic hydrocarbons (SPAHs) and 16 priority PAH species in two coking wastewater treatment plants (WWTPs) (plant E and central WWTP). The measured total concentrations of PAHs and SPAHs in raw wastewater of coking plant E were 3700 and 1200 μg·L, respectively, with naphthalene (1400 μg·L), and fluoranthene (353 μg·L) as dominant PAH species and 2-methylnaphthalene (167 μg·L), anthraquinone (133 μg·L), and 1-methylnaphthalene (132 μg·L) as dominant SPAHs. For the 11 methyl-PAHs (MPAHs), 4 oxygenated-PAHs (OPAHs), and 5 nitrated-PAHs (NPAHs) investigated, the biological wastewater treatment process removed 98.6% MPAHs, 83.9% OPAHs, and 89.1% NPAHs. Mass balance analysis result revealed that transformation was the major mechanism to remove low-molecular-weight (LMW) MPAHs (59.9-77.3%), a large part of OPAHs, including anthraquinone, methylanthraquinone, and 9-fluorenone (46.7-49.6%), and some NPAHs, including 2-nitrofluorene and 9-nitroanthrancene (52.9-59.1%). Adsorption by activated sludge mainly accounted for removing high-molecular-weight (HMW) SPAHs (59.6-71.01%). The relatively high concentrations of SPAHs in excess sludge (15,000 μg·g) and treated effluent (104 μg·L) are of great concern for their potential adverse ecological impacts. SPAHS exhibited similar behaviors in central WWTP, though the influent concentrations were much lower. The concentration levels of SPAHs in the ambient air of coking plant E and central WWTP may also pose potential lung cancer risks (LCR) to the workers through inhalation, where all studied SPAHs except 3-nitrofluoranthene and 7-nitrobenz[a]anthracene exceeded the acceptable cancer risk standards (>10) recommended by U.S EPA. This study could help identify the ecological and healthy risks during coking wastewater treatment and provide useful information for policy-making.
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http://dx.doi.org/10.1016/j.scitotenv.2021.147808DOI Listing
May 2021

Shift in the Dominant Sequence Type of Carbapenem-Resistant Bloodstream Infection from ST11 to ST15 at a Medical Center in Northeast China, 2015-2020.

Infect Drug Resist 2021 21;14:1855-1863. Epub 2021 May 21.

Department of Laboratory Medicine, The First Affiliated Hospital of China Medical University, Shenyang, People's Republic of China.

Objective: To investigate the clinical characteristics and molecular epidemiology of carbapenem-resistant (CRKP) bloodstream infection at a medical center in northeast China, especially after coronavirus disease (COVID-19) pandemic.

Methods: Fifty-one patients were diagnosed with CRKP bloodstream infection between January 2015 and December 2020, among which 42 isolates were available for further study. Species identification and antibiotic susceptibilities were tested with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and VITEK 2 systems. Carbapenemase genes, virulence genes and MLST genes were detected by polymerase chain reaction. Moreover, the string test and serum killing assay were performed to evaluate the virulence of the CRKP isolates.

Results: During the six-year period, the detection rate of CRKP in bloodstream infection showed an increasing trend, with the intensive care unit, hematology and respiratory medicine wards mainly affected. Molecular epidemiology analyses showed that KPC-2 was the dominant carbapenemase gene. In addition, the dominant sequence type (ST) of CRKP shifted from ST11 to ST15 strains, which were all sensitive to amikacin in contrast to the ST11 stains. Furthermore, ST15 CRKP strains were positive for the KfuB virulence gene and more resistant to serum killing compared to the ST11 CRKP strains. Nonetheless, the mortality rate of patients infected with ST11 and ST15 CRKP did not show any significant differences.

Conclusion: A shift in the dominant sequence type of CRKP bloodstream infections from ST11 to ST15 was observed during the years 2015-2020. Compared to ST11, the ST15 CRKP strains showed amikacin sensitivity, positivity for KfuB gene, and serum resistance, which may indicate stronger virulence.
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http://dx.doi.org/10.2147/IDR.S311968DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8158045PMC
May 2021

Biocompatible sulfur nitrogen co-doped carbon quantum dots for highly sensitive and selective detection of dopamine.

Colloids Surf B Biointerfaces 2021 May 23;205:111874. Epub 2021 May 23.

College of Food Science and Engineering, Northwest University, Xi'an 710069, PR China. Electronic address:

In this work, sulfur and nitrogen co-doped carbon quantum dots (S,N-CQDs) were prepared via one-pot hydrothermal treatment of EDTA disodium and sodium sulfide. The prepared S,N-CQDs were characterized by TEM, XRD, FT-IR, XPS, UV-vis absorption and fluorescence spectra to characterize their morphology, crystal structure, functional groups, elemental composition, and optical properties. It was found that S and N elements were successfully doped into the CQDs and the morphology was approximately spherical with an average particle size of 2.16 nm, in which the excitation/emission wavelengths were 350 and 420 nm, respectively. Compared with single element doped CQDs, double element doped CQDs have a higher quantum yield and excellent optical stability. Cell experiments showed that S,N-CQDs had good biocompatibility because they had no obvious toxicity on both normal cell lines and cancer cell lines. More importantly, based on the synergy of static quenching and dynamic quenching, the S,N-CQDs were used as effective fluorescent probes for sensitive detection of DA, with high anti-interference and low limit of detection. Based on the good biocompatibility of S,N-CQDs, the detection of dopamine in actual serum samples were carried out and the results showed an excellent recovery rate. Therefore, this work provides a dopamine sensor with a practical application prospect.
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http://dx.doi.org/10.1016/j.colsurfb.2021.111874DOI Listing
May 2021

[Cannulation of the Portal Vein during Endoscopic Retrograde Cholangiopancreatography in a Patient with Choledocholithiasis].

Korean J Gastroenterol 2021 May;77(5):253-257

Department of Internal Medicine, Presbyterian Medical Center, Jeonju, Korea.

Cannulation of the portal vein is a rare complication of ERCP. This paper reports a case of portal vein catheterization during ERCP in a patient with choledocholithiasis. A 62-year-old man was admitted to the Presbyterian Medical Center with right upper quadrant pain and jaundice. ERCP was performed under the suspicion of obstructive jaundice caused by a radiolucent stone. Bile duct cannulation using a pull-type papillotome was attempted, but it failed. After needle-knife fistulotomy, wire-guided cannulation was performed successfully, and 10 mL contrast was injected. On the other hand, the fluoroscopy image showed that the contrast medium disappeared very quickly. Pure blood was collected when the catheter was aspirated to identify the bile reflux, indicating possible cannulation of the portal vein. The procedure was terminated immediately and abdominal computed tomography showed air in the portal vein. One day after, a follow-up CT scan showed no air in the portal vein. The patient underwent repeated ERCP, and the common bile duct was cannulated. In most cases, isolated portal vein cannulation does not result in severe morbidity. However, it is important to aware of this rare complication so that no further invasive procedure is performed on the patient.
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http://dx.doi.org/10.4166/kjg.2021.030DOI Listing
May 2021

Assessing the effect of treated erythromycin fermentation residue on antibiotic resistome in soybean planting soil: In situ field study.

Sci Total Environ 2021 Jul 9;779:146329. Epub 2021 Mar 9.

State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

As a by-product in the pharmaceutical industry, antibiotic fermentation residue is expected to be able to be utilized after effectively removing the antibiotics. However, evaluation of the effect of fermentation residue application on soil, especially the in situ environmental consequences considering not only the antibiotic resistance gene (ARG) abundance but also the resistome risk, has still not been sufficiently evaluated. Herein, the impact of treated erythromycin fermentation residue (EFR) on the resistome and risk score in soybean planting soil was investigated. Treated EFR application with dosages of 3750 kg (EFR250) and 7500 kg (EFR500) per hm soil did not increase the diversity (Shannon index, 2.84-3.38) or relative abundance (0.086-0.142 copies/16S rRNA gene) of the soil resistome compared with the Control (CK: 2.92-3.2, 0.088-0.096 copies/16S rRNA gene). Soil resistome risk scores calculated by metagenomic assembly, showing the dissemination potential of ARGs, ranged from 22.9 to 25.0, and were also not significantly different between treated EFR amended soil and the Control. Notably, the diversity of the resistome increased at the sprout stage (Mann-Whitney U test, P < 0.05) and the abundance of some ARG types (macrolide-lincosamide-streptogramin, aminoglycoside and tetracycline, etc.) shifted along the course of soybean growth (Kruskal-Wallis test, P < 0.05). Structural equation model analysis showed that the soybean growth period affected the composition of ARGs by affecting the microbial community, which was further supported by Procrustes analysis (P < 0.05) and metagenomic binning. Our findings emphasized that soil ARG abundance and resistome risk did not increase during one-time field application of treated EFR at the studied dosage. Comprehensive consideration including resistome risk and multiple influencing factors also should be given for further assessment of fermentation residue application.
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http://dx.doi.org/10.1016/j.scitotenv.2021.146329DOI Listing
July 2021

The mechanism and function of super enhancer RNA.

Genesis 2021 May 24:e23422. Epub 2021 May 24.

Department of Rheumatology and Immunology, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Super enhancer (SE) is a cluster of enhancers that has a stronger ability to promote transcription compared to the typical enhancer (TE). Similar to TE, which can transcribe enhancer RNA (eRNA), SE produces super enhancer RNA (seRNA). The activation of SE is cell and tissue-specific, and the SE-associated genes are mostly linked to the cell fate. This is demonstrated by the important role-played by SE in the embryonic stem cell (ESC) and multiple cancer development. SeRNA regulates transcription in both cis and trans configuration, and those located in the cytoplasm mediates various cell activities. However, the functions of seRNAs are unclear, and most of them have a synergistic effect with SE and SE-associated genes. In this mini review, we summarized the mechanisms of seRNA and functions of both SE and seRNA.
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http://dx.doi.org/10.1002/dvg.23422DOI Listing
May 2021

Integrated Analysis of Nine Prognostic RNA-Binding Proteins in Soft Tissue Sarcoma.

Front Oncol 2021 7;11:633024. Epub 2021 May 7.

Department of Spine and Orthopedic Oncology, Zhongnan Hospital of Wuhan University, Wuhan, China.

RNA-binding proteins (RBPs) have been shown to be dysregulated in cancer transcription and translation, but few studies have investigated their mechanism of action in soft tissue sarcoma (STS). Here, The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEx) databases were used to identify differentially expressed RBPs in STS and normal tissues. Through a series of biological information analyses, 329 differentially expressed RBPs were identified. Functional enrichment analysis showed that differentially expressed RBPs were mainly involved in RNA transport, RNA splicing, mRNA monitoring pathways, ribosome biogenesis and translation regulation. Through Cox regression analyses, 9 RBPs (BYSL, IGF2BP3, DNMT3B, TERT, CD3EAP, SRSF12, TLR7, TRIM21 and MEX3A) were all up-regulated in STS as prognosis-related genes, and a prognostic model was established. The model calculated a risk score based on the expression of 9 hub RBPs. The risk score could be used for risk stratification of patients and had a high prognostic value based on the receiver operating characteristic (ROC) curve. We also established a nomogram containing risk scores and 9 key RBPs to predict the 1-year, 3-year, and 5-year survival rates of patients in STS. Afterwards, methylation analysis showed significant changes in the methylation degree of BYSL, CD3EAP and MEX2A. Furthermore, the expression of 9 hub RBPs was closely related to immune infiltration rather than tumor purity. Based on the above studies, these findings may provide new insights into the pathogenesis of STS and will provide candidate biomarkers for the prognosis of STS.
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http://dx.doi.org/10.3389/fonc.2021.633024DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8138553PMC
May 2021

Microplastic sampling techniques in freshwaters and sediments: a review.

Environ Chem Lett 2021 May 18:1-28. Epub 2021 May 18.

University of Tehran, Tehran, Islamic Republic of Iran.

Pollution by microplastics is of increasing concern due to their ubiquitous presence in most biological and environmental media, their potential toxicity and their ability to carry other contaminants. Knowledge on microplastics in freshwaters is still in its infancy. Here we reviewed 150 investigations to identify the common methods and tools for sampling microplastics, waters and sediments in freshwater ecosystems. Manta trawls are the main sampling tool for microplastic separation from surface water, whereas shovel, trowel, spade, scoop and spatula are the most frequently used devices in microplastic studies of sediments. Van Veen grab is common for deep sediment sampling. There is a need to develop optimal methods for reducing identification time and effort and to detect smaller-sized plastic particles.
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http://dx.doi.org/10.1007/s10311-021-01227-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8130988PMC
May 2021

Choline attenuates heat stress-induced oxidative injury and apoptosis in bovine mammary epithelial cells by modulating PERK/Nrf-2 signaling pathway.

Mol Immunol 2021 Jul 19;135:388-397. Epub 2021 May 19.

College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China. Electronic address:

Heat stress-induced decline in milk production and mammary glands dysfunction are economically important challenges facing the dairy industry, especially in summer. Choline is an organic water-soluble compound that can regulate a series of vital biological process, including cellular structural integrity and oxidative stress. However, it is unclear whether choline plays an anti-apoptosis and antioxidant effect in heat stress-induced mammary epithelial cells. This study aimed to determine the antioxidant effect of choline on heat stress-induced apoptosis and oxidative stress and its underlying molecular mechanism in bovine mammary epithelial cells (MAC-T cells). The MAC-T cells were divided into four treatment groups: control (37℃), choline (37℃), heat stress (HS, 42℃), and HS + choline. The results showed that heat stress up-regulated the HSP70 and HSP90 expression both in mRNA and protein, enhanced ROS accumulation, increased malondialdehyde (MDA) content, reduced the superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) activity, significantly increased the expression of caspase-3 and upregulated the ratio of Bax/Bcl-2 and ultimately lead to oxidative stress and apoptosis in MAC-T cells. However, choline pretreatment reversed the above phenomenon compared with the HS group. The HS + choline group inhibited heat stress-induced phosphorylation of PERK, nuclear translocation of Nrf-2 and the protein expression of GRP78. In addition, the ratio of Bax/Bcl-2 and the expression of caspase-3 were significantly reduced in HS + choline group, thereby reduced the HS-induced oxidative stress and apoptosis in MAC-T cells. In conclusion, choline attenuates heat stress-induced oxidative stress and apoptosis of MAC-T cells by modulating PERK/Nrf-2 pathway.
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http://dx.doi.org/10.1016/j.molimm.2021.05.002DOI Listing
July 2021

Self-rectifying resistive memory in passive crossbar arrays.

Nat Commun 2021 May 20;12(1):2968. Epub 2021 May 20.

Division of Advanced Materials, Korea Research Institute of Chemical Technology (KRICT) 141 Gajeong-Ro, Yuseong-Gu, Daejeon, Republic of Korea.

Conventional computing architectures are poor suited to the unique workload demands of deep learning, which has led to a surge in interest in memory-centric computing. Herein, a trilayer (HfSiO/AlO/HfSiO)-based self-rectifying resistive memory cell (SRMC) that exhibits (i) large selectivity (ca. 10), (ii) two-bit operation, (iii) low read power (4 and 0.8 nW for low and high resistance states, respectively), (iv) read latency (<10 μs), (v) excellent non-volatility (data retention >10 s at 85 °C), and (vi) complementary metal-oxide-semiconductor compatibility (maximum supply voltage ≤5 V) is introduced, which outperforms previously reported SRMCs. These characteristics render the SRMC highly suitable for the main memory for memory-centric computing which can improve deep learning acceleration. Furthermore, the low programming power (ca. 18 nW), latency (100 μs), and endurance (>10) highlight the energy-efficiency and highly reliable random-access memory of our SRMC. The feasible operation of individual SRMCs in passive crossbar arrays of different sizes (30 × 30, 160 × 160, and 320 × 320) is attributed to the large asymmetry and nonlinearity in the current-voltage behavior of the proposed SRMC, verifying its potential for application in large-scale and high-density non-volatile memory for memory-centric computing.
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http://dx.doi.org/10.1038/s41467-021-23180-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8137934PMC
May 2021

Parabacteroides produces acetate to alleviate heparanase-exacerbated acute pancreatitis through reducing neutrophil infiltration.

Microbiome 2021 05 20;9(1):115. Epub 2021 May 20.

Department of Gastroenterology, Third Military Medical University Second Affiliated Hospital, Chongqing, 400037, China.

Background: The endoglycosidase heparanase which degrades heparan sulfate proteoglycans, exerts a pro-inflammatory mediator in various inflammatory disorders. However, the function and underlying mechanism of heparanase in acute pancreatitis remain poorly understood. Here, we investigated the interplay between heparanase and the gut microbiota in the development of acute pancreatitis.

Methods: Acute pancreatitis was induced in wild-type and heparanase-transgenic mice by administration of caerulein. The differences in gut microbiota were analyzed by 16S ribosomal RNA sequencing. Antibiotic cocktail experiment, fecal microbiota transplantation, and cohousing experiments were used to assess the role of gut microbiota.

Results: As compared with wild-type mice, acute pancreatitis was exacerbated in heparanase-transgenic mice. Moreover, the gut microbiota differed between heparanase-transgenic and wild-type mice. Heparanase exacerbated acute pancreatitis in a gut microbiota-dependent manner. Specially, the commensal Parabacteroides contributed most to distinguish the differences between wild-type and heparanase-transgenic mice. Administration of Parabacteroides alleviated acute pancreatitis in wild-type and heparanase-transgenic mice. In addition, Parabacteroides produced acetate to alleviate heparanase-exacerbated acute pancreatitis through reducing neutrophil infiltration.

Conclusions: The gut-pancreas axis played an important role in the development of acute pancreatitis and the acetate produced by Parabacteroides may be beneficial for acute pancreatitis treatment. Video abstract.
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http://dx.doi.org/10.1186/s40168-021-01065-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8138927PMC
May 2021

Risk factors for postoperative sepsis-induced cardiomyopathy in patients undergoing general thoracic surgery: a single center experience.

J Thorac Dis 2021 Apr;13(4):2486-2494

Department of Cardiology, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai, China.

Background: The current study aimed to investigate the incidence of sepsis-induced cardiomyopathy (SICM) in patients who received general thoracic surgery, along with the risk factors and management strategies for this complication.

Methods: The clinical records of 163 patients with postoperative sepsis were retrospectively reviewed. After propensity score matching, 144 patients were divided into 2 groups by stroke volume: the SICM group (n=72) and the non-SICM group (n=72).

Results: The overall incidence of postoperative SICM was 53.99%. Multiple logistic regression analysis showed that stroke volume and C-reactive protein were independent predictors of mortality in patients with postoperative sepsis. Statistical analysis by -test and χ test indicated that mortality (P=0.000), B-type natriuretic peptide (P=0.001), left ventricular ejection fraction (P=0.000), the mitral peak velocity of early filling/early diastolic mitral annular velocity (E/e') (P=0.049), C-reactive protein (P=0.016), procalcitonin (P=0.013), serum creatinine (P=0.016), platelets (P=0.028), and lactic acid (P=0.002) were significantly associated with the occurrence of postoperative SICM. Among these parameters, B-type natriuretic peptide was identified as the best biomarker for predicting SICM by receiver operating characteristic (ROC) curve analysis.

Conclusions: It is vital to improve the diagnosis and standard management of SICM. A combined strategy comprising early detection of suspected infection, adequate use of antibiotics, close monitoring, effective drainage, and supportive care may improve the outcomes of patients with postoperative SICM.
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http://dx.doi.org/10.21037/jtd-21-492DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107539PMC
April 2021

Fibroblast growth factor 21 (FGF21) is a sensitive marker of osteoporosis in haemodialysis patients: a cross-sectional observational study.

BMC Nephrol 2021 May 19;22(1):183. Epub 2021 May 19.

Institute of Nephrology, Zhong Da Hospital, Southeast University School of Medicine, Nanjing, Jiangsu, China.

Introduction: Osteoporosis is one of the important bone abnormalities in chronic kidney disease-mineral and bone disorder (CKD-MBD) and still lacks a sensitive biomarker to diagnose. Fibroblast growth factor 21 (FGF21) can stimulate bone loss in patients with diabetes and increase in CKD patients. In this study, we investigated whether FGF21 could serve as a biomarker to predict osteoporosis in a haemodialysis cohort.

Methods: We recorded demographic information, biochemical data, and serum FGF21 and FGF23 levels and measured the CT attenuation values of 339 haemodialysis patients from two large medical centres. We assessed the correlation of CT attenuation values with serum FGF21 and FGF23 levels and tested whether they were independent factors for osteoporosis. ROC curves were constructed to compare the prognostic value of FGF21 and FGF23 for osteoporosis.

Results: Based on the CT attenuation value, serum FGF21 levels were higher in our osteoporosis group (median 640.86 pg/ml vs. 245.46 pg/ml, P ˂ 0.01). Meanwhile, FGF21 (r = -0.136, P < 0.05) and FGF23 (r = -0.151, P < 0.05) were both negatively associated with osteoporosis. Moreover, FGF21 (β = -0.067, P < 0.05) was an independent factor for osteoporosis. Furthermore, FGF21 combined with age yielded a marked specificity (90.5 %) and sensitivity (61.8 %) in predicting osteoporosis of haemodialysis patients with less residual renal function.

Conclusions: FGF21 has a positive relationship with the incidence of osteoporosis in patients on haemodialysis. FGF21 combined with age is a good predictive biomarker for osteoporosis in patients on haemodialysis, especially those with less residual renal function.
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http://dx.doi.org/10.1186/s12882-021-02393-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8135985PMC
May 2021

Identification and characterization of factors associated with short stature and pre-shortness in Chinese preschool-aged children.

Endocr Connect 2021 Jun 14;10(6):607-619. Epub 2021 Jun 14.

International Medical Services, China-Japan Friendship Hospital, Beijing, China.

Objectives: We aimed to identify and characterize potential factors, both individually and jointly as a nomogram, associated with short stature and pre-shortness in Chinese preschool-aged children.

Methods: Total of 9501 children aged 3-6 years were recruited from 30 kindergartens in Beijing and Tangshan from September to December 2020 using a stratified random sampling method. Effect-size estimates are expressed as odds ratio (OR) and 95% CI.

Results: The prevalence of short stature and pre-shortness in preschool-aged children was 3.9% (n = 375) and 13.1% (n = 1616), respectively. Factors simultaneously associated with the significant risk for short stature, pre-shortness and both included BMI, paternal height, maternal height, birth weight, birth height, latter birth order (≥2) and less parental patience to children. Besides, breastfeeding duration (≥12 months) was exclusively associated with pre-shortness (OR, 95% CI, P: 1.16, 1.01 to 1.33, 0.037), and childhood obesity with both short stature (3.45, 2.62 to 4.54, <0.001) and short stature/pre-shortness (1.37, 1.15 to 1.64, <0.001). Modeling of significant factors in nomograms had descent prediction accuracies, with the C-index being 77.0, 70.1 and 71.2% for short stature, pre-shortness and both, respectively (all P < 0.001).

Conclusions: Our findings indicate the joint contribution of inherited characteristics, nutrition status from the uterus to childhood, and family psychological environment to short stature and pre-shortness in Chinese preschool-aged children. Further validation in other independent groups is warranted.
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http://dx.doi.org/10.1530/EC-21-0147DOI Listing
June 2021

Long-type double-balloon enteroscopy-assisted ERCP using hand-made accessories in Roux-en-Y hepaticojejunostomy.

Hepatobiliary Pancreat Dis Int 2021 Apr 27. Epub 2021 Apr 27.

Department of Gastroenterology, Ajou University School of Medicine, Suwon, South Korea. Electronic address:

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http://dx.doi.org/10.1016/j.hbpd.2021.04.012DOI Listing
April 2021

The nuclear receptor HNF4 drives a brush border gene program conserved across murine intestine, kidney, and embryonic yolk sac.

Nat Commun 2021 05 17;12(1):2886. Epub 2021 May 17.

Department of Genetics, Human Genetics Institute of New Jersey, Rutgers University, Piscataway, NJ, USA.

The brush border is comprised of microvilli surface protrusions on the apical surface of epithelia. This specialized structure greatly increases absorptive surface area and plays crucial roles in human health. However, transcriptional regulatory networks controlling brush border genes are not fully understood. Here, we identify that hepatocyte nuclear factor 4 (HNF4) transcription factor is a conserved and important regulator of brush border gene program in multiple organs, such as intestine, kidney and yolk sac. Compromised brush border gene signatures and impaired transport were observed in these tissues upon HNF4 loss. By ChIP-seq, we find HNF4 binds and activates brush border genes in the intestine and kidney. H3K4me3 HiChIP-seq identifies that HNF4 loss results in impaired chromatin looping between enhancers and promoters at gene loci of brush border genes, and instead enhanced chromatin looping at gene loci of stress fiber genes in the intestine. This study provides comprehensive transcriptional regulatory mechanisms and a functional demonstration of a critical role for HNF4 in brush border gene regulation across multiple murine epithelial tissues.
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http://dx.doi.org/10.1038/s41467-021-22761-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8129143PMC
May 2021

Disrupted rhythms of life, work and entertainment and their associations with psychological impacts under the stress of the COVID-19 pandemic: A survey in 5854 Chinese people with different sociodemographic backgrounds.

PLoS One 2021 17;16(5):e0250770. Epub 2021 May 17.

Army Medical Center of PLA, Daping Hospital, Army Medical University, Chongqing, P.R. China.

Background & Aim: The coronavirus disease 2019 (COVID-19) pandemic has affected the life and work of people worldwide. The present study aimed to evaluate the rhythm disruptions of life, work, and entertainment, and their associations with the psychological impacts during the initial phase of the COVID-19 pandemic.

Method: A cross-sectional study was conducted from the 10th to 17th March 2020 in China. A structured e-questionnaire containing general information, the Chinese version of Brief Social Rhythm Scale, and Zung's self-rating scales of depression and anxiety (SDS and SAS) was posted and collected online through a public media (i.e. EQxiu online questionnaire platform). Scores in sleeping, getting up, and socializing (SGS) rhythm and eating, physical practice, and entertainment (EPE) rhythm were compared among and between participants with different sociodemographic backgrounds including gender, age, education, current occupation, annual income, health status, and chronic disease status. Correlations of SDS and SAS with SGS-scale and EPE-scale were also analyzed.

Results: Overall, 5854 participants were included. There were significant differences in the scores of SGS-scale and EPE-scale among people with different sociodemographic backgrounds. The scores were significantly higher in the groups with female gender, low education level, lower or higher than average income, poor health status, ages of 26-30 years or older than 61 years, nurses and subjects with divorce or widow status. There were also significant differences in SAS and SDS scores among people with different sociodemographic backgrounds (all P< 0.05). The overall prevalence of depression and anxiety was 24.3% and 12.6%, respectively, with nurses having the highest rates of depression (32.94%) and anxiety (18.98%) among the different occupational groups. SGS-scale was moderately correlated with SDS and SAS, and disruption of SGS rhythm was an independent risk factor for depression and anxiety.

Conclusion: Social rhythm disruption was independently associated with depression and anxiety. Interventions should be applied to people vulnerable to the rhythm disruption during the COVID-19 pandemic.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0250770PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8128272PMC
May 2021