Publications by authors named "Min Wei"

1,124 Publications

  • Page 1 of 1

Comparison of Modified Broström Procedure with or without Suture Tape Augmentation Technique for the Chronic Lateral Ankle Instability.

Biomed Res Int 2022 27;2022:6172280. Epub 2022 Jul 27.

Senior Department of Orthopedics, The Fourth Medical Center of Chinese PLA General Hospital, No. 51, Fucheng Road, Haidian District, 100048 Beijing, China.

Purpose: To compare the clinical outcomes of the modified Broström repair (MBR) with or without suture tape augmentation (STA) for managing the chronic lateral ankle instability.

Methods: 72 patients with chronic lateral ankle instability treated at our hospital from January 2018 to July 2019 were included, with 37 patients receiving the MBR and 35 treated by the MBR with STA. The clinical efficacy of the two techniques was assessed in terms of VAS, AOFAS, and Karlsson scores and by physical examination in follow-ups.

Results: In all 72 patients, operations were successful, and the patients were followed up for 29.3 months on average (range, 24-43 months). There were no significant differences in preoperative pain, AOFAS, and Karlsson scores between the two groups. Compared with preoperative findings, all the functional scores were significantly improved in both groups 3 months after the operation and at the last follow-up. Three months after the operation, the STA group had significantly lower VAS and higher AOFAS scores than the isolated MBR group, suggesting that patients in the STA group suffered less pain and achieved better functional improvement. However, the VAS and functional scores at the last follow-up and the Karlsson score at 3 months postoperatively showed no intragroup difference in both groups.

Conclusion: MBR with or without STA could achieve good results for the treatment of chronic lateral ankle instability. Compared with the widely used MBR, combining with STA may be more effective in promoting rehabilitation in early term.
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http://dx.doi.org/10.1155/2022/6172280DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9348917PMC
July 2022

Research Progress on the Regulation Mechanism of Key Signal Pathways Affecting the Prognosis of Glioma.

Front Mol Neurosci 2022 22;15:910543. Epub 2022 Jul 22.

Graduate School of Dalian Medical University, Dalian, China.

As is known to all, glioma, a global difficult problem, has a high malignant degree, high recurrence rate and poor prognosis. We analyzed and summarized signal pathway of the Hippo/YAP, PI3K/AKT/mTOR, miRNA, WNT/β-catenin, Notch, Hedgehog, TGF-β, TCS/mTORC1 signal pathway, JAK/STAT signal pathway, MAPK signaling pathway, the relationship between BBB and signal pathways and the mechanism of key enzymes in glioma. It is concluded that Yap1 inhibitor may become an effective target for the treatment of glioma in the near future through efforts of generation after generation. Inhibiting PI3K/Akt/mTOR, Shh, Wnt/β-Catenin, and HIF-1α can reduce the migration ability and drug resistance of tumor cells to improve the prognosis of glioma. The analysis shows that Notch1 and Sox2 have a positive feedback regulation mechanism, and Notch4 predicts the malignant degree of glioma. In this way, notch cannot only be treated for glioma stem cells in clinic, but also be used as an evaluation index to evaluate the prognosis, and provide an exploratory attempt for the direction of glioma treatment. MiRNA plays an important role in diagnosis, and in the treatment of glioma, VPS25, KCNQ1OT1, KB-1460A1.5, and CKAP4 are promising prognostic indicators and a potential therapeutic targets for glioma, meanwhile, Rheb is also a potent activator of Signaling cross-talk etc. It is believed that these studies will help us to have a deeper understanding of glioma, so that we will find new and better treatment schemes to gradually conquer the problem of glioma.
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http://dx.doi.org/10.3389/fnmol.2022.910543DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354928PMC
July 2022

EGF promotes PKM2 O-GlcNAcylation by stimulating O-GlcNAc transferase phosphorylation at Y976 and their subsequent association.

J Biol Chem 2022 Aug 2:102340. Epub 2022 Aug 2.

Key Laboratory of Molecular Epigenetics of the Ministry of Education (MOE), Northeast Normal University, 5268 Renmin Street, Changchun, Jilin, 130024, China. Electronic address:

Epidermal growth factor (EGF) is one of the most well-characterized growth factors and plays a crucial role in cell proliferation and differentiation. Its receptor EGFR has been extensively explored as a therapeutic target against multiple types of cancers, such as lung cancer and glioblastoma. Recent studies have established a connection between deregulated EGF signaling and metabolic reprogramming, especially rewiring in aerobic glycolysis, which is also known as the Warburg effect and recognized as a hallmark in cancer. Pyruvate kinase M2 (PKM2) is a rate-limiting enzyme controlling the final step of glycolysis and serves as a major regulator of the Warburg effect. We previously showed that PKM2 T405/S406 O-GlcNAcylation, a critical mark important for PKM2 de-tetramerization and activity, was markedly upregulated by EGF. However, the mechanism by which EGF regulates PKM2 O-GlcNAcylation still remains uncharacterized. Here, we demonstrated that EGF promoted O-GlcNAc transferase (OGT) binding to PKM2 by stimulating OGT Y976 phosphorylation. As a consequence, we found PKM2 O-GlcNAcylation and de-tetramerization were upregulated, leading to a significant decrease in PKM2 activity. Moreover, distinct from PKM2, we observed that the association of additional phosphotyrosine binding proteins with OGT was also enhanced when Y976 was phosphorylated. These proteins included STAT1, STAT3, STAT5, PKCδ, and p85, which are reported to be O-GlcNAcylated. Together, we show EGF-dependent Y976 phosphorylation is critical for OGT-PKM2 interaction and propose that this post-translational modification might be important for substrate selection by OGT.
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http://dx.doi.org/10.1016/j.jbc.2022.102340DOI Listing
August 2022

Electrochemical aptasensor based on the target-induced strand displacement strategy-driven for T-2 toxin detection.

Sci Total Environ 2022 Aug 1:157769. Epub 2022 Aug 1.

School of Food Science and Technology, Henan University of Technology, Zhengzhou, Henan 450001, PR China.

Herein, an aptasensor based on target-induced strand displacement (TISD) strategy was developed for sensitive detection of T-2 toxin. Gold [email protected] aminated manganese dioxide ([email protected]) exhibited excellent electrical conductivity and provided more binding sites for aptamer (Apt). Besides, polyethyleneimine-reduced graphene oxide/gold‑platinum core-shell nanorods composites (PEI-rGO/[email protected] NRs) were used to be carriers for signaling tags, as their sufficiently large specific surface area improved the loading capacity for signal molecules. In the presence of T-2, the Apt sequence was more inclined to form an Apt-T-2 complex, and the cDNA was displaced from the Apt-cDNA duplex, while the signal tag was released, resulting in a weakened MB signal, differential pulse voltammetry (DPV) was used to record the signal change. Under optimal conditions, the signal response of the constructed electrochemical aptasensor exhibited a good linear relationship with the concentration of T-2. The detection limit was 8.74 × 10 ng mLover a wide range of concentration from 5 × 10 ng mL to 5 ng mL. Furthermore, the proposed aptasensor had excellent specificity, good stability and can be well applied to the detection of real samples. It provided a new avenue for the research and development of sensitive aptasensors in food detection and analysis.
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http://dx.doi.org/10.1016/j.scitotenv.2022.157769DOI Listing
August 2022

Lactiplantibacillus plantarum Strain FLPL05 Promotes Longevity in Mice by Improving Intestinal Barrier.

Probiotics Antimicrob Proteins 2022 Aug 3. Epub 2022 Aug 3.

Jiangxi-Oai Joint Research Institute, Nanchang University, 235 Nanjing Donglu, Nanchang, 330047, People's Republic of China.

This study aimed to evaluate the effect of oral administration of probiotic Lactiplantibacillus plantarum FLPL05 on the lifespan and intestinal barrier of aged mice. L. plantarum FLPL05 significantly prolonged the lifespan of naturally aged mice, maintained the integrity of intestinal mucosal barrier, and reduced the inflammation level. The analysis of intestinal microbiota revealed that L. plantarum FLPL05 increased the relative abundance of Firmicutes and decreased the abundance of Bacteroides, accompanied by the increased proportions of Lactobacillus and Desulfovibrio in intestinal microbiota as well as the reduced proportions of Roseburia and Parabacteroides. The intestinal proteomics revealed that the oral administration of L. plantarum FLPL05 significantly upregulated the tight junction and simultaneously inhibited the expression of apoptotic-related proteins. The immunohistochemistry results also indicated that L. plantarum FLPL05 promoted the expression of tight junction proteins (ZO-1 and occludin) and reduced the apoptosis of intestinal cells. In addition, L. plantarum FLPL05 and the fermented supernatant increased the activity of HT-29. L. plantarum FLPL05 prolonged the lifespan by improving the health of the intestinal tract after aging and may be a potential probiotic and nutritional supplement for the elderly people.
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http://dx.doi.org/10.1007/s12602-022-09933-5DOI Listing
August 2022

SYK Is Associated With Malignant Phenotype and Immune Checkpoints in Diffuse Glioma.

Front Genet 2022 15;13:899883. Epub 2022 Jul 15.

Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, China.

Diffuse glioma, the most common intracranial malignant tumor, is characterized by immunosuppression. The prognostic significance and potential therapeutic value of SYK remain obscure. Here, we explored the performance of SYK in predicting patient outcomes and as a therapeutic target. The mRNA expression and clinical data for pancancer and normal tissues and more than 2,000 glioma samples were collected from public databases. The expression level of SYK was evaluated by qPCR and IHC. The prognostic value of SYK was assessed using the Kaplan-Meier curves and univariate and multivariate Cox regression analyses. A sequence of immune and stromal infiltration analyses was calculated based on the ESTIMATE algorithm, ssGSEA algorithm, TIMER, and single-cell analysis. The SYK-related subtypes were identified a Consensus Cluster Plus analysis. SYK was significantly differentially expressed in multiple tumors and normal tissues. Importantly, high-expression SYK was enriched in malignant phenotypes of diffuse gliomas, which was further validated by qPCR and IHC. Survival analysis uncovered that SYK was an independently unfavorable prognostic marker in diffuse glioma. Functional enrichment analysis and immune and stromal infiltration analyses showed that SYK was involved in shaping the immunosuppressive microenvironment of diffuse glioma. Additionally, SYK expression was closely associated with some immune checkpoint molecules and M2 macrophage infiltration, which was validated by IHC and single-cell analysis. Diffuse glioma with Sub1 exhibited a worse prognosis, immunosuppressive microenvironment, and higher expression of immune checkpoint genes. SYK is involved in shaping the immunosuppressive microenvironment and served as a promising prognosis biomarker and immunotherapeutic target for diffuse glioma.
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http://dx.doi.org/10.3389/fgene.2022.899883DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334658PMC
July 2022

Prognostic value and potential mechanism of long non-coding RNA Lnc-SMIM20-1 in acute myeloid leukemia.

Expert Rev Anticancer Ther 2022 Aug 27;22(8):875-885. Epub 2022 Jul 27.

Department of Oncology, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, Guangdong, China.

Objectives: Acute myeloid leukemia (AML) is a common hematologic malignancy with high heterogeneity and poor prognosis. Although long non-coding RNAs (lncRNAs) have been used as biomarkers for tumors, the clinical relevance of numerous lncRNAs in AML remains to be investigated.

Research Design And Methods: Differentially expressed lncRNAs between AML and normal peripheral blood samples were identified using DESeq2. Pan-cancer analysis was performed by GEPIA tool. Kaplan-Meier survival curve was applied for prognosis analysis. KEGG pathway analysis and GSEA were used for functional enrichment. The ceRNA network was constructed by GDCRNAtools.

Results: Lnc-SMIM20-1 was most highly expressed in AML and up-regulated in the TCGA-AML cohort compared to normal tissues. Patients with high expression of Lnc-SMIM20-1 had poor overall prognosis both in the TCGA adult AML cohort and the TARGET pediatric AML cohort, no matter whether they were treated with chemotherapy or allo-HSCT. Lnc-SMIM20-1 might participate in cancer-associated signaling pathways and immune-related signaling pathways by interacting with four microRNAs and 20 mRNAs.

Conclusion: Lnc-SMIM20-1 was up-regulated in AML acting as a stable poor prognostic factor. The prognostic impact of Lnc-SMIM20-1 cannot be overcome by allo-HSCT. Our findings provide insight into the clinical relevance of Lnc-SMIM20-1 in AML; aiming to progress the development of novel therapeutics.
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http://dx.doi.org/10.1080/14737140.2022.2093720DOI Listing
August 2022

Effect of Preheat Temperature and Welding Sequence on the Temperature Distribution and Residual Stress in the Weld Overlay Repair of Hydroturbine Runner.

Materials (Basel) 2022 Jul 13;15(14). Epub 2022 Jul 13.

Hongshanzui Power Plant, Xinjiang Tianfu Energy Co., Ltd., Shihezi 832003, China.

The hydroturbine runner is the core of the whole hydroelectric generating unit, which is employed to transform water energy into mechanical energy. In the process of service, the runner frequently suffers from abrasion due to erosion and cavitation. Defects are usually repaired by welding. To acquire suitable weld cladding repair process parameters, a combination of experimental and numerical simulation was applied to investigate the temperature and weld residual stress distribution in the repair zone under the different welding repair approaches. The results illustrate that the temperature and welding residual stress distribution of the blade and the shroud are out of symmetry, the temperature conduction rate is faster on the blade side, and the high-stress zone is predominantly concentrated in the weld and its adjacent area. When the preheating temperature is up to 150 °C, the peak value of welding residual stress reaches a minimum of 796.29 MPa. The welding sequence can adjust the distribution trend of welding residual stresses. The welding sequence of three-stage welding can effectively reduce the welding residual stresses near the shroud at the water outlet side of the blade. The results of the study will provide theoretical guidance for the welding repair of hydraulic turbine runners.
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http://dx.doi.org/10.3390/ma15144867DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9316777PMC
July 2022

Crucial role of Ca /CN signalling pathway in the antifungal activity of Disenecioyl-cis-Khellactone against Botrytis cinerea.

Pest Manag Sci 2022 Jul 22. Epub 2022 Jul 22.

Jiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, 210014, China.

Background: Botrytis cinerea causes grey mould and is one of the most destructive fungal pathogens affecting important fruit and vegetable crops. In preliminary studies, we found that Disenecioyl-cis-Khellactone(DK) had strong antifungal activity against several fungi species including B. cinerea (EC =11.0 μg·mL ). In this study, we aimed to further evaluate the antifungal activity of DK against B. cinerea and determine the role of Ca /CN signalling pathway on its antifungal effect.

Results: DK was effective against B. cinerea in both in vitro and in vivo assays. Exogenous Ca reduced the antifungal activity of DK. The combination of DK and cyclosporine A (CsA) did not exhibit an additive effect against B. cinerea. In contrast to CsA, DK reduced the intracellular Ca concentration in B. cinerea. DK bound to calcineurin A (cnA) and up-regulated the expression of PMC1 and PMR1 genes. Moreover, DK sensitivity of △bccnA significantly decreased compared with that of Bc05.10 strain.

Conclusion: DK is a promising lead compound for developing fungicides against B. cinerea. The Ca /CN signalling pathway plays a crucial role in the DK antifungal activity, and cnA is one of the targets of DK against B. cinerea. DK directly reacts with cnA, which up-regulates the transcription of Ca /CN-dependent target genes PMC1 and PMR1, decreasing the intracellular Ca concentration and disturbing the intracellular Ca balance, leading to cell death. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/ps.7085DOI Listing
July 2022

Effect of radiation processing on phenolic antioxidants in cereal and legume seeds: A review.

Food Chem 2022 Jul 12;396:133661. Epub 2022 Jul 12.

Biotechnology and Nuclear Technology Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan 610061, PR China.

Phenolic compounds in cereal and legume seeds show numerous benefits to human health mainly because of their good antioxidant capacity. However, long-term storage and some improper preservation may reduce their antioxidant potential. It is necessary to retain or modify the phenolic antioxidants with improved technology before consumption. Radiation processing is usually applied as a physical method to extend the shelf life and retain the quality of plant produce. However, the effect of radiation processing on phenolic antioxidants in cereal and legume seeds is still not well understood. This review summarizes recent research on the effect of radiation, including ionizing and nonionizing radiation on the content and profile of phenolic compounds, and antioxidant activities in cereal and legume seeds, the influencing factors and possible mechanisms are also discussed. The article will improve the understanding of radiation effect on phenolic antioxidants, and promote the radiation modification of natural phenolic compounds in cereal and legume seeds and other sources.
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http://dx.doi.org/10.1016/j.foodchem.2022.133661DOI Listing
July 2022

Antibacterial metal nanoclusters.

J Nanobiotechnology 2022 Jul 16;20(1):328. Epub 2022 Jul 16.

Institute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, 310058, Hangzhou, China.

Combating bacterial infections is one of the most important applications of nanomedicine. In the past two decades, significant efforts have been committed to tune physicochemical properties of nanomaterials for the development of various novel nanoantibiotics. Among which, metal nanoclusters (NCs) with well-defined ultrasmall size and adjustable surface chemistry are emerging as the next-generation high performance nanoantibiotics. Metal NCs can penetrate bacterial cell envelope more easily than conventional nanomaterials due to their ultrasmall size. Meanwhile, the abundant active sites of the metal NCs help to catalyze the bacterial intracellular biochemical processes, resulting in enhanced antibacterial properties. In this review, we discuss the recent developments in metal NCs as a new generation of antimicrobial agents. Based on a brief introduction to the characteristics of metal NCs, we highlight the general working mechanisms by which metal NCs combating the bacterial infections. We also emphasize central roles of core size, element composition, oxidation state, and surface chemistry of metal NCs in their antimicrobial efficacy. Finally, we present a perspective on the remaining challenges and future developments of metal NCs for antibacterial therapeutics.
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http://dx.doi.org/10.1186/s12951-022-01538-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9287886PMC
July 2022

Identification of novel candidate genes and small molecule drugs in ovarian cancer by bioinformatics strategy.

Transl Cancer Res 2022 Jun;11(6):1630-1643

Medical Frontier Innovation Research Center, The Central Laboratory, The First Hospital of Lanzhou University, The First Clinical Medical College of Lanzhou University, Lanzhou, China.

Background: Ovarian cancer (OC) is the most lethal type of malignancies in the female reproductive system. This study aimed to identify novel biomarkers and potential small molecule drugs in OC by integrating two expression profile datasets.

Methods: GSE18520 and GSE14407 from the Gene Expression Omnibus (GEO) database were selected and the overlapped differentially expressed genes (DEGs) were detected. The Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genome (KEGG) pathway enrichment analysis were performed to establish the protein-protein interaction (PPI) network of DEGs and identified the hub genes. Gene Expression Profiling Interactive Analysis (GEPIA), Oncomine database and The Human Protein Atlas (HPA) were used to validate the expression of the identified hub genes. The prognostic value of these hub genes were evaluated by the Kaplan Meier plotter online tool. The expression of NCAPG was further explored by immunohistochemistry in our OC tissues. Moreover, CMap database was used to look for prospective small compounds with therapeutic efficacy based on OC RNA-seq.

Results: A total of 433 DEGs were identified. The DEGs were mainly enriched in negative regulation of transcription and pathways in cancer. A PPI network was constructed with 344 nodes and 1,596 interactions. The top ten module genes were chosen as hub genes. Among which, survival analysis showed that patients with high expression of and had poorer survival results than those with low expression. These six genes were all overexpressed in OC tissue by means of bioinformatics analysis. In our clinical patients, the expression rate of NCAPG in OC tissues was significantly higher than that in benign serous ovarian cystadenoma and borderline serous ovarian cystadenoma tissues. Meanwhile, several small molecules with potential therapeutic efficacy against OC were identified in our study.

Conclusions: By means of bioinformatics analysis, we identified six real hub genes and indicated a group of candidate small molecule drugs as adjunctive agents for OC. They could be the potential novel biomarkers for the diagnosis and promising therapeutic targets of OC.
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http://dx.doi.org/10.21037/tcr-21-2890DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9273707PMC
June 2022

Exposure to fluopimomide at sublethal doses causes oxidative stress in Caenorhabditis elegans regulated by insulin/insulin-like growth factor 1-like signaling pathway.

Environ Toxicol 2022 Jul 14. Epub 2022 Jul 14.

Key Laboratory of Pesticide Toxicology & Application Technique, College of Plant Protection, Shandong Agricultural University, Tai'an, 271018, People's Republic of China.

Fluopimomide is an innovative pesticide, widely used for agricultural pest management; however, little is known about its effect on non-target organisms. This study was designed to assess the potential risk of fluopimomide and the molecular mechanisms using Caenorhabditis elegans, a common model animal. The oxidative stress-related indicators were analyzed in C. elegans after exposure to fluopimomide for 24 h at three sublethal doses (0.2, 1.0, and 5.0 mg/L). The results demonstrated that sublethal exposure to fluopimomide adversely affected the nematodes growth, locomotive behaviors, reproduction, and lifespan, accompanying with enhanced of reactive oxygen species (ROS) generation, lipid and lipofuscin accumulation, and malondialdehyde content. In addition, exposure to fluopimomide significantly inhibited antioxidant systems including superoxide dismutase, catalase, glutathione S-transferase, and glutathione in the nematodes. Moreover, the expression of oxidative stress-related genes of sod-3, hsp-16.1, gst-4, ctl-2, daf-16, and daf-2 were significantly down-regulated, while the expression of skn-1 was significantly up-regulated. Further evidence revealed that daf-16 and skn-1 mutant strains of C. elegans significantly decreased ROS production upon fluopimomide exposure compared with the wild-type nematodes. Overall, our findings indicated that exposure to fluopimomide at sublethal doses caused oxidative damage, mainly associated with insulin/IGF-1-like signaling pathway in C. elegans. This is the first report of potential toxic effects of fluopimomide even at low concentrations, providing a new insight into the mechanisms of toxicity to C. elegans by fluopimomide.
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http://dx.doi.org/10.1002/tox.23616DOI Listing
July 2022

Ultrasensitive detection of patulin based on a Ag-driven one-step dual signal amplification.

J Hazard Mater 2022 Jul 4;438:129530. Epub 2022 Jul 4.

School of Food Science and Technology, Henan University of Technology, Zhengzhou, Henan 450001, PR China.

Due to improper storage, the presence of patulin in fruits poses a threat to food safety. Herein, a one-step dual amplification strategy-based electrochemical aptasensor was proposed for patulin detection. Silver-palladium nanoparticles (AgPdNPs) with a hollow and branched structure were used as a supporting material for thionine to provide numerous attachment sites. AuNFs/g-CN was employed as an electrode modification material, which has been demonstrated to facilitate electron transport and improve signal label loading capacity. Ag ions were released in the presence of patulin, activating the Ag-DNAzyme on the electrode surface. The formed Ag-DNAzymes further cyclically cleaved the substrate DNA, and the released sequences were used as a new trigger to mediate the secondary recirculation. This one-step dual amplification strategy enabled double target recycling without additional procedures. The signal cascade amplification through dual target recycling, was thus available for trace detection of patulin. Under the optimal conditions, the electrochemical aptasensor achieved a satisfactory linear range from 5.0 × 10 μg L to 50 μg L with a detection limit of 0.92 fg·mL for the determination of patulin. In addition, the aptasensor exhibited favorable selectivity, reproducibility, repeatability and long-term stability, and thus can be employed for patulin detection in apple juice samples, providing excellent choice for the detection of trace patulin.
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http://dx.doi.org/10.1016/j.jhazmat.2022.129530DOI Listing
July 2022

Metal-Acid Bifunctional Catalysts toward Tandem Reaction: One-Step Hydroalkylation of Benzene to Cyclohexylbenzene.

ACS Appl Mater Interfaces 2022 Jul 6;14(28):31998-32008. Epub 2022 Jul 6.

State Key Laboratory of Chemical Resource Engineering, Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology, Beijing 100029, P.R. China.

The one-step hydroalkylation of benzene to cyclohexylbenzene (CHB) is a technically challenging and economically interesting reaction with great industrial importance, where bifunctional catalysts play a crucial role in such a tandem reaction. In this work, we report HPWO (HPW) modified Ni nanoparticles (NPs) supported on mixed metal oxides (Ni/MMOs), which are featured by HPW species localized on the surface of Ni NPs (denoted as HPW-Ni/MMOs). The optimal catalyst (0.3HPW-Ni/MMOs) exhibits a satisfactory catalytic performance toward benzene hydroalkylation to CHB with a CHB yield of up to 41.2%, which is the highest standard among previously reported catalysts to date. A combination investigation based on HR-TEM, XPS, XANES, and FT-IR verified the electron transfer from the W atom to the adjacent Ni atom, which facilitated the formation and desorption of cyclohexene (CHE) from Ni followed by the alkylation reaction of benzene and CHE at the interfacial Brønsted (B) acid sites of HPW, accounting for the significantly enhanced catalytic behavior. It is proposed that the HPW-Ni interface structure in HPW-Ni/MMOs samples provides unique adsorption sites for benzene and CHE with a moderate adsorption strength, which serve as the intrinsic active center for this reaction: the Ni site promotes the hydrogenation of benzene to CHE, while the B acid site in HPW facilitates the alkylation of CHE and benzene to produce CHB. This work provides a fundamental understanding of the metal-acid synergistic catalysis toward the hydroalkylation reaction, which can be extended to the design and preparation of high-performance catalysts used in tandem reactions.
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http://dx.doi.org/10.1021/acsami.2c07074DOI Listing
July 2022

Deubiquitinase OTUD6A promotes breast cancer progression by increasing TopBP1 stability and rendering tumor cells resistant to DNA-damaging therapy.

Cell Death Differ 2022 Jun 29. Epub 2022 Jun 29.

State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, China.

The DNA damage response (DDR) is critical for maintaining cellular homeostasis and genome integrity. Mounting evidence has shown that posttranslational protein modifications play vital roles in the DDR. In this study, we showed that deubiquitinase OTUD6A is involved in the DDR and is important for maintaining genomic stability. Mechanistically, in response to DNA damage, the abundance of OTUD6A was increased; meanwhile, PP2A interacted with OTUD6A and dephosphorylated OTUD6A at sites S70/71/74, which promoted nuclear localization of OTUD6A. Subsequently, OTUD6A was recruited to the damage site, where it interacted with TopBP1 and blocked the interaction between TopBP1 and its ubiquitin E3 ligase UBR5, decreasing K48-linked polyubiquitination and increasing the stability of TopBP1. OTUD6A depletion impaired CHK1 S345 phosphorylation and blocked cell cycle progression under DNA replication stress. Consistently, knockout of OTUD6A rendered mice hypersensitive to irradiation, shortened survival, and inhibited tumor growth by regulating TopBP1 in xenografted nude mice. Moreover, OTUD6A is expressed at high levels in breast cancer, and OTUD6A overexpression promotes cell proliferation, migration and invasion, indicating that dysregulation of OTUD6A expression contributes to genomic instability and is associated with tumor development. In summary, this study demonstrates that OTUD6A plays a critical role in promoting tumor cell resistance to chemoradiotherapy by deubiquitinating and stabilizing TopBP1.
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http://dx.doi.org/10.1038/s41418-022-01036-6DOI Listing
June 2022

[Clinical outcomes of arthroscopic single-tunnel double-line technique in treating tibial intercondylar eminence fractures].

Authors:
Yang Luo Min Wei

Zhongguo Gu Shang 2022 Jun;35(6):555-9

Senior Department of Orthopaedics, the Forth Medical Center of People's Liberation Army General Hospital, Beijing 100037, China.

Objective: To evaluate clinical efficacy of single-tunnel double-line technique with tendoscopy in treating tibial intercondylar eminence fractures.

Methods: From January 2016 to April 2020, 22 patients with tibial intercondylar eminence fractures were admitted, including 14 males and 8 females with a mean age of (34.8±5.9) years old ranging from 26 to 45 years old. The mean duration from injury to surgery was(11.2±4.1) days(ranged, 5 to15 d). All patients were treated with arthroscopic single-tunnel double-line technique. The fracture healing was evaluated according to X-ray. The Lysholm score, IKDC 2000 score and the anterior drawer test were used to evaluate efficacy before operation and at the latest follow-up.

Results: All patients were followed up, and the duration ranged from 12 to 75 months, with an average of(34.6±13.0) months. Lysholm score was (89.60±2.89) points at the latest follow-up, showing significant difference when compared with preoperative score which was (30.80±9.55)points(=9.67, <0.01). IKDC 2000 score was(80.00±6.17) points at the latest follow-up, showing significant difference when compared with preoperative score which was(24.60±7.21)points(=11.41, <0.01). One patient showed weakly positive of drauer test.

Conclusion: Single-channel double-line technique could effectively fix bone block of intercondylar eminence with advantage of easy operation and minimally invasive, which could be applied to various types of tibial intercondylar eminence fractures.
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http://dx.doi.org/10.12200/j.issn.1003-0034.2022.06.010DOI Listing
June 2022

Prognostic value of PD-L1 and Siglec-15 expression in patients with nasopharyngeal carcinoma.

Sci Rep 2022 Jun 21;12(1):10401. Epub 2022 Jun 21.

Department of Oncology, The Affiliated Hospital of Southwest Medical University, Luzhou, 646000, China.

Sialic acid-binding immunoglobulin-like lectin 15 (Siglec-15) might be involved in the activation of important pathways related to tumor immune escape, along with programmed death-ligand 1 (PD-L1). Here, we aimed to investigate the correlation between the expression of Siglec-15 and PD-L1 in nasopharyngeal carcinoma (NPC) patients. We determined the expression of PD-L1 via immunohistochemical staining and that of Siglec-15 via immunofluorescence staining in 182 NPC tissue samples. A significant correlation was identified between the PD-L1 and Siglec-15 expression (P = 0.000). Moreover, Kaplan-Meier survival curves showed that PD-L1 expression was associated with improved overall survival (OS) (P = 0.025) and Siglec-15 expression was associated with improved distant failure-free survival (D-FFS) (P = 0.048). Moreover, multivariate Cox analysis showed that PD-L1 and Siglec-15 were independent predictors of OS (P = 0.020) and D-FFS (P = 0.047), respectively. The results of the log-rank test and Cox regression analyses showed that patients exhibiting no PD-L1/Siglec-15 expression had significant advantages regarding OS, compared to other groups (P = 0.037). PD-L1 and Siglec-15 may represent novel biomarkers for predicting the prognosis of NPC patients. Siglec-15 may be considered as a potential target for the development of therapeutics for NPC treatment in the future.
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http://dx.doi.org/10.1038/s41598-022-13997-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9213533PMC
June 2022

Linking oxidative DNA lesion 8-OxoG to tumor development and progression.

Yi Chuan 2022 Jun;44(6):466-477

The Key Laboratory of Molecular Epigenetics of Ministry of Education, College of Life Sciences, Northeast Normal University, Changchun 130024, China.

Cells of the aerobic metabolic organism are inevitably subjected to the damage from reactive oxygen species (ROS). ROS cause multiple forms of DNA damage, among which the oxidation product of guanine G 8-hydroxyguanine (8-oxoG) is the most frequent DNA oxidative damage, recognized by the specific glycosidase OGG1 that initiates the base excision repair pathway. If left unrepaired, 8-oxoG may pair with A instead of C, leading to a mutation of G: C to T: A during replication. Thus, the accumulation of 8-oxoG or the abnormal OGG1 repair is thought to affect gene function, which in turn leads to the development of tumor or aging-related diseases. However, a series of recent studies have shown that 8-oxoG tends to be produced in regulatory regions of the genome. 8-oxoG can be regarded as an epigenetic modification, while OGG1 is a specific reader of this information. Substrate recognition, binding or resection by OGG1 can cause DNA conformation changes or affect histone modifications, causing up-regulation or down-regulation of genes with different properties. Thus, in addition to the potential genotoxicity, the association of guanine oxidative damage with development of tumors is closely related to its aberrant initiation of gene expression through epigenetic mechanisms. In this review, we summarize the underlying mechanism of 8-oxoG and repair enzyme OGG1 in tumor development and progression, with aims to interpret the relationship between DNA oxidative damage and tumor from a new perspective, and provide new ideas and targets for tumor treatment.
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http://dx.doi.org/10.16288/j.yczz.22-067DOI Listing
June 2022

Tumor-microenvironment triggered signal-to-noise boosting nanoprobes for NIR-IIb fluorescence imaging guided tumor surgery and NIR-II photothermal therapy.

Biomaterials 2022 Jun 16;287:121636. Epub 2022 Jun 16.

Key Laboratory of Design and Assembly of Functional Nanostructures, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, 350002, PR China; The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou, 350025, PR China; Department of Translational Medicine, Xiamen Institute of Rare Earth Materials, Chinese Academy of Sciences, Xiamen, 361024, PR China. Electronic address:

High quantum yield quantum dots (QDs) with the emission in the sub-second near infrared window (NIR-IIb, 1500-1700 nm) can afford higher resolution, a deeper penetration depth and zero auto-fluorescence for bio-imaging. However, low tumor accumulation, the rapid renal clearance and potential toxicity impeding their biomedical applications. Here, we report a tumor microenvironment responsive hollowed virus-bionic MnO nanoshell with IR1061 loading in the cavity and QDs ([email protected]) anchoring on the surface for precise NIR-IIb fluorescence imaging guided tumor surgery and efficient NIR-II photothermal therapy. This QDs based nanoprobe could efficiently adhere on tumor cells to realize efficient tumor tissue accumulation. NIR-IIb fluorescence of tumor margin could be successfully delineating after extracellular weak acid triggered MnO biodegradation for IR1061 release with remarkable NIR-IIb signal-to-noise boosting. Then, it could facilitate complete dissection of various tumor models with the assistance of NIR-IIb fluorescence imaging. Moreover, the fascinating efficacy for micro-metastasis eradication via NIR-II photothermal effects can be achieved under NIR-IIb fluorescence imaging guidance. Specifically, in combination with negligible system toxicity, our nanoprobes showed great potential as a versatile NIR-IIb fluorescent imaging platform for precise tumor surgery and tumor therapy guidance for future clinical translation.
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http://dx.doi.org/10.1016/j.biomaterials.2022.121636DOI Listing
June 2022

Long non-coding RNA LOC644135 is a potential prognostic indicator in cytogenetically normal acute myeloid leukemia.

Expert Rev Hematol 2022 Jul 22;15(7):657-665. Epub 2022 Jun 22.

Department of Oncology, the Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, Guangdong, China.

Objectives: Acute myeloid leukemia (AML) is a hematological malignancy with highly clinical heterogeneity resulting in poor outcomes. We aim to identify novel prognostic lncRNA in AML expecting to provide new clues for therapy in AML.

Methods: Three cohorts were enrolled in this study. Differentially expressed lncRNAs between TCGA-AML cohort and GTEx cohort was identified by DESeq2. The relationship between expression level of LOC644135 and prognosis in AML was analyzed by multiple methods.

Results: Pan-cancer analysis indicated that LOC644135 was most highly expressed in AML across 33 types of cancer. Patients with high expression of LOC644135 had poor overall prognosis in both TCGA-AML cohort and the TARGET-AML cohort. Especially, high expression of LOC644135 indicated inferior overall survival and event-free survival in CN-AML patients in the TCGA-AML cohort. Besides, CN-AML patients had higher expression of LOC644135 than normal samples. Multivariable analysis suggested that LOC644135 was an independent prognostic factor in AML. GSEA analysis showed that LOC644135 was associated with some immune-related pathways. Besides, high expression of LOC644135 was associated with less infiltration of CD8 T cell.

Conclusion: Our findings indicated that LOC644135 was an independent prognostic factor in AML and provided a new idea in the development of therapy in AML.
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http://dx.doi.org/10.1080/17474086.2022.2091542DOI Listing
July 2022

Genome-Wide Identification and Functional Characterization of Auxin Response Factor (ARF) Genes in Eggplant.

Int J Mol Sci 2022 Jun 1;23(11). Epub 2022 Jun 1.

State Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an 271018, China.

Auxin response factors (ARFs) are important plant transcription factors that are differentially expressed in response to auxin and various abiotic stresses. ARFs play important roles in mediating plant growth and stress responses; however, these factors have not been studied in eggplants. In this study, genome-wide identification and the functional analysis of the ARF gene family in eggplants ( L.) were performed. A total of 20 () genes were identified and phylogenetically classified into three groups. Our analysis revealed four functional domains and 10 motifs in these proteins. Subcellular localization showed that the SmARFs localized in the nucleus. To investigate the biological functions of the under 2,4-D and salt stress treatments, quantitative real-time RT-PCR (qRT-PCR) was conducted. Most genes exhibited changes in expression in response to 2,4-D treatments in the flowers, especially and . All genes quickly responded to salt stress, except and in leaves, and in roots, and , , and in stems. These results helped to elucidate the role of ARFs in auxin signaling under 2,4-D and salt stress in eggplants.
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http://dx.doi.org/10.3390/ijms23116219DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9181582PMC
June 2022

Ultrasound microbubble-mediated delivery of ANLN silencing-repressed EZH2 expression alleviates cervical cancer progression.

Tissue Cell 2022 Aug 27;77:101843. Epub 2022 May 27.

Ultrasonic Diagnosis Center, Northwest Women's and Children's Hospital, Xi'an 710061, China. Electronic address:

Ultrasound-targeted microbubble destruction (UTMD) is a new gene therapy method that uses ultrasound and microbubbles carrying target genes to achieve gene transfection. However, whether UTMD-mediated ANLN silencing transfection helps to restrain the growth of cervical cancer (CC) is obscure. ANLN level in tumor tissues, adjacent tissues, and cells was tested using the database, qRT-PCR, and western blot. The optimal concentration of SF6 was determined by MTT assay. Mechanical index (MI) was selected by flow cytometry. After transfection with liposome or UTMD-mediated liposome, cell function experiments, qRT-PCR, and western blot were employed to assess CC cell biological behaviors and EZH2 level. Epithelial-mesenchymal transition (EMT)-related marker and apoptosis-related marker expressions were examined utilizing qRT-PCR and western blot. 10% SF6 and MI of 0.28 were selected for subsequent tests. ANLN was highly expressed in CC and cells. The transfection efficiency of the UTMD-siANLN group was higher than that of the L-siANLN group. Moreover, the repression of UTMD-siANLN on CC cell malignant phenotypes was stronger than L-siANLN. UTMD-siANLN attenuated EZH2 expression in CC cells. The modulatory role of UTMD-siANLN on EMT- and apoptosis-related markers was reversed by EZH2 overexpression. UTMD can improve the efficiency of siANLN transfection into CC cells to induce suppression of CC cell malignant phenotypes, which may become a new target of gene therapy for CC.
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http://dx.doi.org/10.1016/j.tice.2022.101843DOI Listing
August 2022

Highly-efficient RuNi single-atom alloy catalysts toward chemoselective hydrogenation of nitroarenes.

Nat Commun 2022 Jun 8;13(1):3188. Epub 2022 Jun 8.

State Key Laboratory of Chemical Resource Engineering, Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology, Beijing, 100029, P. R. China.

The design and exploitation of high-performance catalysts have gained considerable attention in selective hydrogenation reactions, but remain a huge challenge. Herein, we report a RuNi single atom alloy (SAA) in which Ru single atoms are anchored onto Ni nanoparticle surface via Ru-Ni coordination accompanied with electron transfer from sub-surface Ni to Ru. The optimal catalyst 0.4% RuNi SAA exhibits simultaneously improved activity (TOF value: 4293 h) and chemoselectivity toward selective hydrogenation of 4-nitrostyrene to 4-aminostyrene (yield: >99%), which is, to the best of our knowledge, the highest level compared with reported heterogeneous catalysts. In situ experiments and theoretical calculations reveal that the Ru-Ni interfacial sites as intrinsic active centers facilitate the preferential cleavage of N-O bond with a decreased energy barrier by 0.28 eV. In addition, the Ru-Ni synergistic catalysis promotes the formation of intermediates (CHNO* and CHNOH*) and accelerates the rate-determining step (hydrogenation of CHNOH*).
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http://dx.doi.org/10.1038/s41467-022-30536-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9178046PMC
June 2022

Cell-based reporter assays for measurements of antibody-mediated cellular cytotoxicity and phagocytosis against SARS-CoV-2 spike protein.

J Virol Methods 2022 Sep 6;307:114564. Epub 2022 Jun 6.

State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen, Fujian, PR China; National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Life Sciences, Xiamen University, Xiamen, Fujian, PR China.

The COVID-19 pandemic caused by SARS-CoV-2 infections has led to excess deaths worldwide. Neutralizing antibodies (nAbs) against viral spike protein acquired from natural infections or vaccinations contribute to protection against new- and re-infections. Besides neutralization, antibody-mediated cellular cytotoxicity (ADCC) and phagocytosis (ADCP) are also important for viral clearance. However, due to the lack of convenient methods, the ADCC and ADCP responses elicited by viral infections or vaccinations remain to be explored. Here, we developed cell-based assays using target cells stably expressing SARS-CoV-2 spikes and Jurkat-NFAT-CD16a/CD32a effector cells for ADCC/ADCP measurements of monoclonal antibodies and human convalescent COVID-19 plasmas (HCPs). In control samples (n = 190), the specificity was 99.5% (95%CI: 98.4-100%) and 97.4% (95%CI: 95.1-99.6%) for the ADCC and ADCP assays, respectively. Among 87 COVID-19 HCPs, 83 (sensitivity: 95.4%, 95%CI: 91.0-99.8%) and 81 (sensitivity: 93.1%, 95%CI: 87.8-98.4%) showed detectable ADCC (titer range: 7.4-1721.6) and ADCP activities (titer range: 4-523.2). Notably, both ADCC and ADCP antibody titers positively correlated with the nAb titers in HCPs. In summary, we developed new tools for quantitative ADCC and ADCP analysis against SARS-CoV-2, which may facilitate further evaluations of Fc-mediated effector functions in preventing and treating against SARS-CoV-2.
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http://dx.doi.org/10.1016/j.jviromet.2022.114564DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9167684PMC
September 2022

A ratiometric fluorescent aptamer homogeneous biosensor based on hairpin structure aptamer for AFB1 detection.

J Fluoresc 2022 Jun 4. Epub 2022 Jun 4.

College of Food Science and Technology, Henan Key Laboratory of Cereal and Oil Food Safety Inspection and Control, Henan University of Technology, 450001, Zhengzhou, PR China.

On the basis of aptamer (Apt) with hairpin structure and fluorescence resonance energy transfer (FRET), a ratio fluorescent aptamer homogeneous sensor was prepared for the determination of Aflatoxin B (AFB1). Initially, the Apt labeled simultaneously with Cy5, BHQ2, and cDNA labeled with Cy3 were formed a double-stranded DNA through complementary base pairing. The fluorescence signal of Cy3 and Cy5 were restored and quenched respectively. Thus, the ratio change of F to F was used to realized the detection of AFB1 with wider detection range and lower limit of detection (LOD). The response of the optimized protocol for AFB1 detection was wider linear range from 0.05 ng/mL to 100 ng/mL and the LOD was 12.6 pg/mL. The sensor designed in this strategy has the advantages of simple preparation and fast signal response. It has been used for the detection of AFB1 in labeled corn and wine, and has good potential for application in real samples.
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http://dx.doi.org/10.1007/s10895-022-02972-9DOI Listing
June 2022

Comparison of foramen magnum decompression with and without duraplasty in the treatment of adult Chiari malformation Type I: a meta-analysis and systematic review.

Turk Neurosurg 2022 Jan 10. Epub 2022 Jan 10.

xuanwu hospital.

Aim: Some meta-analyses have focused on foramen magnum decompression with duraplasty (PFDD) and without duraplasty (PFD) in paediatric or mixed populations. Nevertheless, no meta-analysis has evaluated adults only. This study aimed to include new relevant findings in a systematic review to provide the first comparison of PFDD and PFD in adult CM-I.

Material And Methods: We retrospectively searched Web of Science, PubMed, Embase and ClinicalTrials.gov to summarize all relevant published papers on adults. A systemic review was adopted to evaluate clinical or radiological improvement, surgical complications, and reoperation rates between the PFD and PFDD groups.

Results: Nine papers containing information on 497 adult participants met the criteria. PFDD was related to a lower revision rate (RR=2.96, 95% CI: 1.34-6.51, P=0.007) but a higher complication rate (RR=0.35, 95% CI: 0.22-0.55, P 0.00001). No significant difference was noted between PFD and PFDD in terms of overall symptom improvement (RR=0.93, 95% CI: 0.84-1.03, P = 0.17) or syringomyelia reduction (RR=0.84, 95% CI: 0.63-1.12, P = 0.24). No significant difference in symptom improvement was observed between patients with syringomyelia (RR=0.86, 95% CI: 0.69-1.08, P = 0.20) and patients without syringomyelia (RR=0.94, 95% CI: 0.68-1.30, P = 0.73).

Conclusion: This systematic review of observational studies reveals that PFDD may provide lower revision rates but pose a higher risk than PFD in the management of CM-I in adults. However, PFD is similar to PFDD in clinical and radiological improvements.
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http://dx.doi.org/10.5137/1019-5149.JTN.35727-21.5DOI Listing
January 2022

Identification and relative expression analysis of gene family in pepper.

3 Biotech 2022 Jun 24;12(6):137. Epub 2022 May 24.

College of Horticulture, Gansu Agricultural University, Lanzhou, 730070 China.

Fructokinase is the main catalytic enzyme for fructose phosphorylation and can also act as a glucose receptor and signal molecule to regulate the metabolism of plants, which plays an important role in plant growth and development. In this study, the gene family and their molecular characteristics are systematically identified and analyzed, and the specific expression of under different tissues, abiotic stresses and hormone treatments were explored. Nine genes were authenticated in pepper genome database, which were dispersedly distributed on eight reference chromosomes and predicted to localize in the cytoplasm. Many -acting elements that respond to light, different stresses, hormones and tissue-specific expression were found in the promoters of . FRK proteins of four species including , , and were divided into four groups via phylogenetic analysis. The collinearity analysis showed that there were two collinear gene pairs between and . In addition, it was significantly found that expressed far higher in flower than other tissues, and the relative expression of was gradually enhanced with the development of flower buds in fertile accessions, 8B, R1 and F. Nevertheless, hardly expressed in all stages of cytoplasmic male sterile lines. Based on the quantitative real-time PCR, most of genes showed significant up-regulation under low-temperature, NaCl and PEG6000 treatments. On the contrary, the expression levels of most revealed a various trend in response to hormone treatments (IAA, ABA, GA, SA and MeJA). This study systematically analyzed gene family and studied its expression pattern, which lay the foundation of CaFRK genes cloning and functional verification response to abiotic stresses, and provides new insights into exploring the genes on the pollen development in pepper.

Supplementary Information: The online version contains supplementary material available at 10.1007/s13205-022-03196-1.
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http://dx.doi.org/10.1007/s13205-022-03196-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9130412PMC
June 2022

Prediction of the Short-Term Therapeutic Effect of Anti-VEGF Therapy for Diabetic Macular Edema Using a Generative Adversarial Network with OCT Images.

J Clin Med 2022 May 19;11(10). Epub 2022 May 19.

Department of Ophthalmology, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250012, China.

Purpose: To generate and evaluate individualized post-therapeutic optical coherence tomography (OCT) images that could predict the short-term response of anti-vascular endothelial growth factor (VEGF) therapy for diabetic macular edema (DME) based on pre-therapeutic images using generative adversarial network (GAN).

Methods: Real-world imaging data were collected at the Department of Ophthalmology, Qilu Hospital. A total of 561 pairs of pre-therapeutic and post-therapeutic OCT images of patients with DME were retrospectively included in the training set, 71 pre-therapeutic OCT images were included in the validation set, and their corresponding post-therapeutic OCT images were used to evaluate the synthetic images. A pix2pixHD method was adopted to predict post-therapeutic OCT images in DME patients that received anti-VEGF therapy. The quality and similarity of synthetic OCT images were evaluated independently by a screening experiment and an evaluation experiment.

Results: The post-therapeutic OCT images generated by the GAN model based on big data were comparable to the actual images, and the response of edema resorption was also close to the ground truth. Most synthetic images (65/71) were difficult to differentiate from the actual OCT images by retinal specialists. The mean absolute error (MAE) of the central macular thickness (CMT) between the synthetic OCT images and the actual images was 24.51 ± 18.56 μm.

Conclusions: The application of GAN can objectively demonstrate the individual short-term response of anti-VEGF therapy one month in advance based on OCT images with high accuracy, which could potentially help to improve treatment compliance of DME patients, identify patients who are not responding well to treatment and optimize the treatment program.
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http://dx.doi.org/10.3390/jcm11102878DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9144043PMC
May 2022

The effect of talus osteochondral defects of different area size on ankle joint stability: a finite element analysis.

BMC Musculoskelet Disord 2022 May 27;23(1):500. Epub 2022 May 27.

National Clinical Research Center for Orthopaedics, Sports Medicine & Rehabilitation, Beijing, China.

Background: Osteochondral lesion of the talus (OLT) is one of the most common ankle injuries, which will lead to biomechanical changes in the ankle joint and ultimately affect ankle function. Finite element analysis (FEA) is used to clarify the effect of talus osteochondral defects on the stability of the ankle joint at different depths. However, no research has been conducted on talus osteochondral defect areas that require prompt intervention. In this research, FEA was used to simulate the effect of the area size of talus osteochondral defect on the stress and stability of the ankle joint under a specific depth defect.

Methods: Different area sizes (normal, 2 mm* 2 mm, 4 mm* 4 mm, 6 mm* 6 mm, 8 mm* 8 mm, 10 mm* 10 mm, and 12 mm* 12 mm) of the three-dimensional finite element model of osteochondral defects were established. The model was used to simulate and calculate joint stress and displacement of the articular surface of the distal tibia and the proximal talus when the ankle joint was in the heel-strike, midstance, and push-off phases.

Results: When OLT occurred, the contact pressure of the articular surface, the equivalent stress of the proximal talus, the tibial cartilage, and the talus cartilage did not change significantly with an increase in the size of the osteochondral defect area when the heel-strike phase was below 6 mm * 6 mm. Gradual increases started at 6 mm * 6 mm in the midstance and push-off phases. Maximum changes were reached when the defect area size was 12 mm * 12 mm. The same patterns were observed in the talus displacement.

Conclusions: The effect of the defect area of the ankle talus cartilage on the ankle biomechanics is evident in the midstance and push-off phases. When the size of the defect reaches 6 mm * 6 mm, the most apparent change in the stability of the ankle joint occurs, and the effect does not increase linearly with the increase in the size of the defect.
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http://dx.doi.org/10.1186/s12891-022-05450-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9137113PMC
May 2022
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