Publications by authors named "Min Peng"

256 Publications

The complete mitochondrial genome of the mudskipper, (Gobiiformes, Oxudercidae) from Beibu Bay.

Mitochondrial DNA B Resour 2021 Apr 5;6(4):1337-1338. Epub 2021 Apr 5.

Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Nanning, China.

The mudskipper, (), is an amphibious fish that lives in the intertidal mudflats. It is a cultured economic fish with nutritional and pharmacological value. Here, we report the complete mitochondrial genome sequence of , which is 17,111 base pairs (55.3% A + T content) in length and consists of 13 protein-coding genes, 22 transfer RNAs, 2 ribosomal RNAs, and a 1453 bp D-loop region. The complete mitochondrial genome of will provide useful genetic information for future phylogenetic and taxonomic classification of .
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http://dx.doi.org/10.1080/23802359.2021.1909433DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8023618PMC
April 2021

The complete mitochondrial genome of yellowfin seabream, (Percoiformes, Sparidae) from Beibu Bay.

Mitochondrial DNA B Resour 2021 Mar 31;6(4):1313-1314. Epub 2021 Mar 31.

Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning, China.

The yellowfin seabream, Houttuyn 1782, is a commercially and ecologically important species and a good model for studies of sexual differentiation. In this study, the complete mitochondrial genome of has been determined, which is 16,635 base pairs (54.3% A + T content) in length and consists of 13 protein-coding genes, 22 transfer RNAs, two ribosomal RNAs, and a 948 bp D-loop region. The phylogenetic analyses showed that has a close relationship with Bleeker 1854.
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http://dx.doi.org/10.1080/23802359.2021.1907804DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8018338PMC
March 2021

Effect of dipeptidyl peptidase-4 inhibitors on the progression of atherosclerosis in patients with type 2 diabetes mellitus: A meta-analysis of randomised controlled trials.

Int J Clin Pract 2021 Apr 5:e14213. Epub 2021 Apr 5.

Department of Traditional Chinese Medicine, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China.

Objectives: Type 2 diabetes mellitus (T2DM) can accelerate the clinical process of atherosclerosis (AS). Dipeptidyl peptidase-4 inhibitors (DPP-4Is) have potential anti-AS effects. And, we completed a meta-analysis of the changes in carotid intima-media thickness (CIMT), flow-mediated dilation (FMD) and pulse wave velocity (PWV) of DPP-4Is to research the effect of DPP-4Is in the progression of AS in T2DM patients.

Materials And Methods: We included RCTs that evaluated the impact of DPP-4Is on CIMT, FMD and PWV compared to other treatments from PubMed, Cochrane trials and Embase database before October 31, 2020. We selected the random-effect model and calculated the weighted mean difference (WMD) to evaluate the effect of CIMT, FMD and PWV in T2DM patients.

Results: Through the meta-analysis, we found that DPP-4Is can significantly reduce CIMT in T2DM patients (WMD = -0.036, 95% CI:-0.055 to-0.017; P ≤ .001). Based on the subgroup analysis, we found that CIMT was significantly decreased in patients with greater than 12 months of intervention and without cardiovascular diseases. Besides, we also found that DPP-4Is had a no significant efficacy on the improvement of FMD in T2DM patients (WMD = 0.635, 95% CI: -0.112 to 1.383, P = .097). Our subgroup analysis showed that for T2DM patients who have cardiovascular diseases, DPP-4Is can significantly increase their FMD. In addition, we also found that DPP-4Is had an insignificant influence on PWV in T2DM patients (WMD = 0.424, 95% CI: -0.198 to 1.046, P = .18), but SGLT2 inhibitors may reduce the PWV of T2DM patients.

Conclusions: DPP-4Is can alleviate the development of AS in T2DM patients to a certain extent by reducing CIMT. And, we believe that long-term use of DPP-4Is may be more helpful to alleviate the atherosclerotic development of T2DM without obvious cardiovascular history.
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http://dx.doi.org/10.1111/ijcp.14213DOI Listing
April 2021

Screening of Hub Genes Associated with Pulmonary Arterial Hypertension by Integrated Bioinformatic Analysis.

Biomed Res Int 2021 22;2021:6626094. Epub 2021 Mar 22.

China State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China.

Background: Pulmonary arterial hypertension (PAH) is a disease or pathophysiological syndrome which has a low survival rate with abnormally elevated pulmonary artery pressure caused by known or unknown reasons. In addition, the pathogenesis of PAH is not fully understood. Therefore, it has become an urgent matter to search for clinical molecular markers of PAH, study the pathogenesis of PAH, and contribute to the development of new science-based PAH diagnosis and targeted treatment methods.

Methods: In this study, the Gene Expression Omnibus (GEO) database was used to downloaded a microarray dataset about PAH, and the differentially expressed genes (DEGs) between PAH and normal control were screened out. Moreover, we performed the functional enrichment analyses and protein-protein interaction (PPI) network analyses of the DEGs. In addition, the prediction of miRNA and transcriptional factor (TF) of hub genes and construction miRNA-TF-hub gene network were performed. Besides, the ROC curve was used to evaluate the diagnostic value of hub genes. Finally, the potential drug targets for the 5 identified hub genes were screened out.

Results: 69 DEGs were identified between PAH samples and normal samples. GO and KEGG pathway analyses revealed that these DEGs were mostly enriched in the inflammatory response and cytokine-cytokine receptor interaction, respectively. The miRNA-hub genes network was conducted subsequently with 131 miRNAs, 7 TFs, and 5 hub genes (CCL5, CXCL12, VCAM1, CXCR1, and SPP1) which screened out via constructing the PPI network. 17 drugs interacted with 5 hub genes were identified.

Conclusions: Through bioinformatic analysis of microarray data sets, 5 hub genes (CCL5, CXCL12, VCAM1, CXCR1, and SPP1) were identified from DEGs between control samples and PAH samples. Studies showed that the five hub genes might play an important role in the development of PAH. These 5 hub genes might be potential biomarkers for diagnosis or targets for the treatment of PAH. In addition, our work also indicated that paying more attention on studies based on these 5 hub genes might help to understand the molecular mechanism of the development of PAH.
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http://dx.doi.org/10.1155/2021/6626094DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8010527PMC
March 2021

[Predictive factors of asymptomatic airway hyperresponsiveness in chronic rhinosinusitis with nasal polyps].

Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2021 Apr;35(4):345-350

Department of Otorhinolaryngology,Fenyang Hospital Affiliated to Shanxi Medical University,Fenyang,032200,China.

To explore the predictive factors for asymptomatic airway hyperresponsiveness(AAHR) in patients with chronic rhinosinusitis with nasal polyps(CRSwNP). A total of 76 CRSwNP patients who were hospitalized in the Department of Otorhinolaryngology of Fenyang Hospital affiliated to Shanxi Medical University from May 2016 to October 2020 were retrospectively analyzed, including 40 patients in AAHR group and 36 patients in non-airway hyperresponsiveness(non-airway hyperresponsiveness, NAHR) group. The clinical symptoms, CT score of paranasal sinuses, eosinophil(EOS) count in peripheral blood and nasal polyp tissue were compared and analyzed. Logistic regression was used to analyze the risk factors of AAHR, and receiver operating characteristics curve was used to judge the predictive value of the parameters. Compared with NAHR group, AAHR group had more symptoms of mouth breathing and postnasal drip, higher total score of CT in ethmoid sinus, sphenoid sinus, olfactory cleft, and more EOS count in peripheral blood and nasal polyp tissue. The differences were statistically significant. There was a positive correlation between EOS count in peripheral blood and in nasal polyp tissue(=0.324, <0.01). Postnasal drip, high posterior ethmoid sinus(PE) score and nasal polyp tissue EOS count were risk factors for AAHR. The predictive value of nasal polyp tissue EOS count was higher than that of PE score(AUC=0.786 and 0.685, respectively). When the PE score was ≥1.5, the sensitivity was 80.0% and the specificity was 55.6%. When the nasal polyp tissue EOS count was ≥5.67/HPF, the sensitivity was 82.5% and the specificity was 66.7%. The occurrence of AAHR in patients with CRSwNP was related to clinical symptoms, paranasal sinus CT score, peripheral and nasal polyp tissue EOS count. PE score and nasal polyp tissue EOS count can be used to predict AAHR, however nasal polyp tissue EOS has higher predictive value.
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http://dx.doi.org/10.13201/j.issn.2096-7993.2021.04.014DOI Listing
April 2021

[Factors Affecting the Translocation and Accumulation of Cadmium in a Soil-Crop System in a Typical Karst Area of Guangxi Province, China].

Huan Jing Ke Xue 2021 Mar;42(3):1514-1522

Institute of Geophysical & Geochemical Exploration, Chinese Academy of Geological Sciences, Langfang 065000, China.

To understand the main factors influencing the translocation and accumulation of cadmium (Cd) in soil-crop systems in typical karst areas, 68 sets of paddy soil and rice grain samples were collected in Guangxi Province. These were used to analyze Cd concentrations and soil properties (pH, organic matter (OM) content, oxide content, and texture). Spearman's correlation coefficients and principal component analysis (PCA) were used to examine the effects of soil properties on Cd concentrations and identify the main influencing factors. The studied soils were highly enriched in iron oxide (TFeO), aluminum oxide (AlO), and manganese oxide (MnO) compared to background levels, with average concentrations of 20.2%, 19.0%, and 0.2%, respectively. However, the soils are relatively depleted in silica (SiO), with an average concentration of 41.0%. The soils are strongly weathered and leached in study area, giving rise to rich occurrences of Fe-Mn nodules. The concentrations of TFeO and MnO in the study soils were significantly correlated with soil Cd, rice seed Cd, and the Cd bioconcentration factor (BCF). The PCA analysis further showed that TFeO and MnO in soils were the main factors affecting the migration and enrichment of Cd while soil pH, OM, and AlO had less of an influence. Furthermore, SiO and soil texture indirectly affected the migration and enrichment of Cd. It is suggested that the Fe-Mn nodules effectively adsorb and immobilize Cd in the study area soils, acting as a heavy metal scavenger that reduced the biological accessibility of Cd.
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http://dx.doi.org/10.13227/j.hjkx.202007138DOI Listing
March 2021

[Geochemical Survey Method of Land Quality in Land Parcel Scale City: A Case Study of the Initial Area of the Xiong'an New District].

Huan Jing Ke Xue 2021 Apr;42(4):1989-2002

Institute of Geophysical & Geochemical Exploration, Chinese Academy of Geological Sciences, Langfang 065000, China.

In order to evaluate the land quality geochemical survey achievement in the service of the accurate management of urban land resources, the initial area of the Xiong'an New District as urbanization pathfinder in China is chosen as the research subject. The sample points were set by differential classification, and the spatial interpolation accuracy of the soil elements at a plot scale and a quantitative assessment of the consistency of the land plot (pattern spot) prediction evaluation were studied under the conditions of different sampling densities. The regional geochemical variation values randomly distributed on the plane can be reflected quantitatively by differential classification sampling, which can meet the basic demand of the quality attribute of a single plot (map spot) by the accurate management of urban land resources. The spatial variability of soil elements is mostly middle to moderate, and Cd, Cu, Pb, Hg, Se, N, P, and other elements of high spatial variability are affected by human industrial and agricultural production activities. Under the same sampling density, the larger the element variation coefficient, the worse the spatial interpolation accuracy. Although the interpolation accuracy of the same element index is affected by the sampling density, the increase in the sampling density could not identify the continuous component on the structure of the soil element content. The soil environment is clean, and the heavy metal content is lower than the GB15618-2018 standard. The interpolation results are basically consistent with the grading results of the measured values, while the contents of N, P, and K of the nutrient indices vary greatly, and the predicted and measured geochemical grades of the plots (map spot) differ substantially under the influence of factors such as human disturbance and spatial variability. The quantitative evaluation of the six different sampling densities indicates that the 16 points·km sampling density adopted in the geochemical survey and evaluation of urban land quality can satisfy the needs of an accurate control of urban land resources in the study area and similar areas. The research can provide key technologies to support and serve the accurate management of urban land resources for geochemical surveys and the evaluation of land quality in land parcel scale cities.
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http://dx.doi.org/10.13227/j.hjkx.202008133DOI Listing
April 2021

[Changes in Concentrations and Pollution Levels of Trace Elements of Floodplain Sediments of Poyang Lake Basin in Recent Twenty Years].

Huan Jing Ke Xue 2021 Apr;42(4):1724-1738

Institute of Geophysical & Geochemical Exploration, Chinese Academy of Geological Sciences, Langfang 065000, China.

Investigating the pollution conditions of trace elements in the Poyang Lake basin, identifying the pollution sources, evaluating the ecological risks play an important part in formulating the effective strategies for protecting the basin ecosystem. To understand the concentration characteristics, pollution levels, and ecological risks of twelve trace elements (Cd, Hg, Pb, As, Cu, Zn, Cr, Ni, Mn, Sb, W, and Sn) in the floodplain sediments of the five main tributaries in Poyang Lake basin in the past twenty years, we used the enrichment factor (EF) and modified pollution index (MPI) to quantify the pollution levels of the trace elements, and analyzed and discussed the pollution sources in the areas with significant pollution level changes. The results indicated that the concentrations of cadmium, zinc, manganese, tungsten and lead in the floodplain sediments of the Poyang Lake basin during the past twenty years have increased by 134%, 26%, 41%, 25%, and 8%, respectively, and mercury, arsenic, chromium, nickel, antimony, and tin have decreased by 35%, 15%, 22%, 10%, 14%, and 13%, respectively, while copper has not changed significantly. The concentrations of cadmium in the Raohe River, Ganjiang River, and Xinjiang River basins have increased by 331%, 151%, and 107%, while mercury decreased in the Fuhe River, Ganjiang River, and Xiushui River basins by 87%, 41%, and 40%, respectively. The classification results based on the EF indicated that the changes in the pollution levels of trace elements in the Poyang Lake basin were characterized by the aggravation of cadmium pollution and the degradation of mercury pollution, the pollution level of cadmium in the Ganjiang River basin was upgraded to moderate and significant, the pollution level of cadmium in the Le'an River was upgraded to very high, and the pollution level of mercury in the Fuhe River basin was reduced to unpolluted or minimal. The MPI classification results established that the increase in the cadmium pollution had intensified the comprehensive pollution in the Poyang Lake basin, causing the proportion of severely and heavily polluted sites to be raised from 17% to 33%. The MRI classification results also demonstrated that the proportion of very high ecological risk sites have increased from 11% to 22%. The element with the highest MRI contribution rate changed from mercury to cadmium, which was caused by the reduction of the ecological risk level from very high to moderate. The results demonstrated that the significant pollution changes in the trace elements in the floodplain sediments of the Poyang Lake basin were primarily caused by human activities, such as mineral exploitation, smelting, and industrial and agricultural production. Our findings can provide scientific evidence for the prevention and control of river pollution and the optimal management of a watershed ecosystem.
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http://dx.doi.org/10.13227/j.hjkx.202008083DOI Listing
April 2021

Clinical features and long-term outcomes of interstitial lung disease with anti-neutrophil cytoplasmic antibody.

BMC Pulm Med 2021 Mar 16;21(1):88. Epub 2021 Mar 16.

Department of Respiratory and Critical Care Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, No. 1 Shuai Fu Yuan, Dongcheng District, Beijing, 100730, China.

Background: Patients with interstitial lung disease (ILD) are occasionally positive for anti-neutrophil cytoplasmic antibodies (ANCAs). Differences between ILDs secondary to microscopic polyangiitis (MPA) and isolated ANCA-positive idiopathic interstitial pneumonia (IIP) remain unclear. The aim of this study was to explore the differences in clinical features and outcomes between MPA-associated ILDs and isolated ANCA-positive IIPs.

Methods: We reviewed 1338 ILDs patients with available ANCA results and retrospectively analysed 80 patients who were ANCA-positive. MPA-associated ILDs (MPA-ILDs group) and isolated ANCA-positive IIPs (ANCA-IIPs group) were compared.

Results: Among 80 patients with ANCA-positive ILDs, 31 (38.75%) had MPA-ILDs, and 49 (61.25%) had isolated ANCA-positive IIPs. Compared with ANCA-IIPs group, patients in MPA-ILDs group had a higher proportion of fever (p = 0.006) and higher neutrophil count (p = 0.011), erythrocyte sedimentation rate (ESR) (p < 0.001) and C-reactive protein (CRP) (p = 0.005). Multivariable analysis showed that ESR level was an independent risk factor for mortality in all 80 ANCA-positive ILDs patients (HR 1.028, p = 0.001). Survival in MPA-ILDs group was lower than that in ANCA-IIPs group, and further stratified analysis revealed that ANCA-IIPs patients with elevated ESR or CRP had a worse prognosis than those with normal inflammation markers, with 5-year cumulative survival rates of 60.00%, 86.90% and 100.00% in MPA-ILDs and ANCA-IIPs with and without elevated inflammation markers, respectively.

Conclusions: Among patients with ANCA-positive ILDs, the prognoses of ANCA-IIPs with normal inflammation markers, ANCA-IIPs with elevated inflammation markers and MPA-ILDs were sequentially poorer. Therefore, stratified treatment should be considered in the management of ILDs patients positive for ANCAs.
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http://dx.doi.org/10.1186/s12890-021-01451-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7968287PMC
March 2021

Risk factors associated with loss to follow-up of breast cancer patients: A retrospective analysis.

Breast 2021 Jun 2;57:36-42. Epub 2021 Mar 2.

Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, 510120, China; Breast Tumor Center, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, Guangdong, 510120, PR China; Disease Registry Center, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, Guangdong, 510120, PR China. Electronic address:

Background: Loss to follow-up (LTFU) during post-operative surveillance of breast cancer patients is detrimental. The pattern of LTFU and its risk factors in Chinese breast cancer patients remains unknown.

Method: Eligible non-metastatic breast cancer patients who underwent surgery at our institution between 2009 and 2012 were included. The clinicopathological features, as well as the LTFU status, were retrieved from the REDCap database. LTFU was defined as the absence of patients for at least 12 months since her last contact. 5-year LTFU was defined as the LTFU status of each patients at 5 years after surgery. The incidence and potential risk factors of LTFU were analyzed. A LTFU-risk score was developed to quantify the risk of LTFU.

Results: A total of 1536 patients with breast cancer were included, and 411(26.8%) patients were 5-year LTFU. 198 patients were LTFU in the first year. Univariate and multivariate analysis revealed that age (younger and older), a lack of medical insurance, longer distance from residence to the hospital, pathology (DCIS/Paget's/Phyllodes), lymph node metastasis, the absence of endocrine therapy and fewer than five contact numbers were significantly and independently associated with the risk of LTFU. A LTFU-risk score was developed and was predictive of LTFU.

Conclusions: A series of risk factors were significantly associated with post-operative LTFU of breast cancer patients. Patients with different risks of LTFU could possibly be identified, and surveillance plans could be individualized for different patients, so as to effectively reduce the overall LTFU rate, and optimize the allocation of medical resources.
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http://dx.doi.org/10.1016/j.breast.2021.02.012DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7970119PMC
June 2021

A simple synthesis of [RuCl(NHC)(p-cymene)] complexes and their use in olefin oxidation catalysis.

Dalton Trans 2021 Mar 1;50(11):3959-3965. Epub 2021 Mar 1.

Department of Chemistry and Centre for Sustainable Chemistry Ghent University, Krijgslaan 281, S-3, 9000 Ghent, Belgium.

A simple and efficient synthetic route to [RuCl(NHC)(p-cymene)] and [Ru(CO)(NHC)(p-cymene)] complexes making use of a weak base, under aerobic conditions, is reported. This method enables access to a series of NHC-ruthenium compounds with moderate to good yields under mild conditions. The Ru pre-catalysts were successfully used in olefin oxidation catalysis at low catalyst loading and reach complete conversion in short times.
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http://dx.doi.org/10.1039/d1dt00030fDOI Listing
March 2021

Efficacy and safety of thyroxine therapy on patients with heart failure and subclinical hypothyroidism: A protocol for systematic review and meta-analysis.

Medicine (Baltimore) 2021 Jan;100(3):e23947

Department of Traditional Chinese Medicine, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, ShanDong, China.

Background: Subclinical hypothyroidism (SCH) can increase the risk of heart failure (HF) clinically. However, thyroxine therapy for patients with HF and SCH has the risk of developing tachyarrhythmias. At present, there is no sufficient evidence-based medical evidence for levothyroxine in the therapy of this situation, and the treatment issue is still controversial. Therefore, our meta-analysis aims to assess the effectiveness and safety of thyroxine therapy for patients with HF and SCH.

Methods: We searched the related randomized controlled trials that have been published in the following 7 electronic databases: PubMed, Cochrane Library, EMBASE, Chongqing VIP, China National Knowledge Infrastructure, Chinese biomedical literature database, and Wan Fang database. The treatment group was treated with routine HF therapy plus thyroxine, while the control group was treated with HF routine therapy. Main outcome measures effective rate and New York Heart Association classification; Secondary outcome measures included: left ventricular ejection fraction, quality of life score, brain natriuretic peptide / N-terminal pro brain natriuretic peptide, 6-minute walk test, and adverse events. After screening studies and extracting data, we will use Cochrane collaborative tools to evaluate the risk of bias to assess the methodological quality of the included randomized controlled trials. We will use STATA 14.0 software for data synthesis and statistical analysis. Both subgroup analysis and sensitivity analysis will be used to detect potential sources of heterogeneity. In addition, we will use sensitivity analysis to test the stability of the outcomes. If possible, we will perform a funnel chart and Eggers test evaluate publication bias. The quality of the evidence will be evaluated through the grades of recommendations assessment, development, and evaluation system.

Results: Our findings will be published in peer-reviewed journals.

Conclusion: This research will provide evidence about the efficacy and safety of thyroxine in the treatment of patients with HF and SCH. Objective to provide evidence-based medicine basis for thyroxine treatment of patients with SCH and HF.

Registration Number: INPLASY2020100062.
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http://dx.doi.org/10.1097/MD.0000000000023947DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7837952PMC
January 2021

Reply to Chen.

Clin Infect Dis 2021 02;72(3):538-539

School of Nursing, Hong Kong Polytechnic University, Hong Kong, China.

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http://dx.doi.org/10.1093/cid/ciaa690DOI Listing
February 2021

Chemerin reverses the malignant phenotype and induces differentiation of human hepatoma SMMC7721 cells.

Arch Pharm Res 2021 Feb 27;44(2):194-204. Epub 2021 Jan 27.

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China.

Chemerin exhibits an inhibitory effect on hepatocellular carcinoma; however, the underlying mechanism is unclear. Here, low chemerin expression was confirmed in samples of liver cancer patients and hepatoma cells. Chemerin altered hepatoma cell morphology but had no effect on normal hepatocytes. Chemerin inhibited proliferation of several human hepatoma cell lines. Real-time PCR detection of hepatocellular carcinoma markers showed that mRNA levels of albumin and A-type gamma-glutamyl transferase increased whereas those of alpha-fetoprotein, alkaline phosphatase, B-type gamma-glutamyl transferase, insulin-like growth factor II, and human telomerase reverse transcriptase decreased in chemerin-treated SMMC7721 cells. Western blotting revealed that chemerin up-regulated albumin and vimentin expressions, and downregulated alpha-fetoprotein expression. Phosphorylated STAT3 was significantly up-regulated, whereas phosphorylated ERK and AKT were significantly downregulated by chemerin. Chemerin decreased phosphorylated ERK and AKT expression and the cell proliferation induced by PI3K activator 740 Y-P but could not significantly alter phosphorylated STAT3 expression and the cell growth induced by STAT3 inhibitor NSC74859. In conclusion, chemerin reversed the malignant phenotype and induced SMMC7721 cell differentiation by inhibiting MAPK/ERK and PI3K/AKT signaling; growth inhibition by chemerin is not directly related to the JAK/STAT signaling pathway. Our study provides novel evidence that chemerin could be utilized for liver cancer treatment.
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http://dx.doi.org/10.1007/s12272-021-01311-zDOI Listing
February 2021

Combination of Immune Checkpoint Inhibitors and Radiotherapy for Advanced Non-Small-Cell Lung Cancer and Prostate Cancer: A Meta-Analysis.

J Oncol 2021 5;2021:6631643. Epub 2021 Jan 5.

Department of Oncology Center, Renmin Hospital of Wuhan University, Wuhan 430060, China.

Objectives: Immune checkpoint inhibitors (ICI) combined with radiotherapy (RT) have emerged as a breakthrough therapy in the treatment of various cancers. The combination has a strong rationale, but data on their efficacy and safety are still limited. Hence, we comprehensively searched the database and performed this study to elucidate the clinical manifestations of this combined strategy.

Methods: We performed a meta-analysis of randomized trials that compared ICI plus RT with placebo plus RT or ICI alone for the treatment of advanced nonsmall-cell lung cancer (NSCLC) and prostate cancer. The outcomes included overall survival (OS), progression-free survival (PFS), disease control rate (DCR), and treatment-related adverse events. A fixed-effects or random-effects model was adopted depending on between-study heterogeneity.

Results: Three trials involving 1584 patients were included. ICI plus RT was significantly associated with improvement of OS (hazard ratio [HR] = 0.81, 95% confidence interval [CI] = 0.70-0.94, =0.004), PFS (HR = 0.64; 95% CI 0.56-0.72, < 0.00001), and DCR (relative risk [RR] = 1.38; 95% CI 1.03-1.84, =0.03). A significant predictor for PFS with the combination of ICI and RT was age, as a significant improvement in PFS (HR = 0.49; 95% CI 0.37-0.64, < 0.00001) was observed in NSCLC patients aged under 65 years. In safety analyses, patients receiving ICI plus RT had a significantly higher incidence of dyspnea (RR = 2.43; 95% CI 1.16-5.08, =0.02) and pneumonitis of grade 3 or higher (RR = 2.78; 95% CI 1.32-5.85, =0.007).

Conclusion: The combination of ICI and RT was associated with improved OS, PFS, and DCR. Patients under 65 years will be the dominant beneficiaries. However, the incidence of dyspnea and pneumonia of grade 3 or higher also increased, which deserves our vigilance.
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http://dx.doi.org/10.1155/2021/6631643DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7803407PMC
January 2021

Glycolysis fuels phosphoinositide 3-kinase signaling to bolster T cell immunity.

Science 2021 01;371(6527):405-410

Immunology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA.

Infection triggers expansion and effector differentiation of T cells specific for microbial antigens in association with metabolic reprograming. We found that the glycolytic enzyme lactate dehydrogenase A (LDHA) is induced in CD8 T effector cells through phosphoinositide 3-kinase (PI3K) signaling. In turn, ablation of LDHA inhibits PI3K-dependent phosphorylation of Akt and its transcription factor target Foxo1, causing defective antimicrobial immunity. LDHA deficiency cripples cellular redox control and diminishes adenosine triphosphate (ATP) production in effector T cells, resulting in attenuated PI3K signaling. Thus, nutrient metabolism and growth factor signaling are highly integrated processes, with glycolytic ATP serving as a rheostat to gauge PI3K-Akt-Foxo1 signaling in the control of T cell immunity. Such a bioenergetic mechanism for the regulation of signaling may explain the Warburg effect.
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http://dx.doi.org/10.1126/science.abb2683DOI Listing
January 2021

Effects of Survival Motor Neuron Protein on Germ Cell Development in Mouse and Human.

Int J Mol Sci 2021 Jan 11;22(2). Epub 2021 Jan 11.

Institute of Biotechnology, National Taiwan University, Taipei 106, Taiwan.

Survival motor neuron (SMN) is ubiquitously expressed in many cell types and its encoding gene, survival motor neuron 1 gene (SMN1), is highly conserved in various species. SMN is involved in the assembly of RNA spliceosomes, which are important for pre-mRNA splicing. A severe neurogenic disease, spinal muscular atrophy (SMA), is caused by the loss or mutation of SMN1 that specifically occurred in humans. We previously reported that SMN plays roles in stem cell biology in addition to its roles in neuron development. In this study, we investigated whether SMN can improve the propagation of spermatogonia stem cells (SSCs) and facilitate the spermatogenesis process. In in vitro culture, SSCs obtained from SMA model mice showed decreased growth rate accompanied by significantly reduced expression of spermatogonia marker promyelocytic leukemia zinc finger (PLZF) compared to those from heterozygous and wild-type littermates; whereas SMN overexpressed SSCs showed enhanced cell proliferation and improved potency. In vivo, the superior ability of homing and complete performance in differentiating progeny was shown in SMN overexpressed SSCs in host seminiferous tubule of transplant experiments compared to control groups. To gain insights into the roles of SMN in clinical infertility, we derived human induced pluripotent stem cells (hiPSCs) from azoospermia patients (AZ-hiPSCs) and from healthy control (ct-hiPSCs). Despite the otherwise comparable levels of hallmark iPCS markers, lower expression level of was found in AZ-hiPSCs compared with control hiPSCs during in vitro primordial germ cell like cells (PGCLCs) differentiation. On the other hand, overexpressing in AZ-hiPSCs led to increased level of pluripotent markers such as OCT4 and KLF4 during PGCLC differentiation. Our work reveal novel roles of SMN in mammalian spermatogenesis and suggest new therapeutic targets for azoospermia treatment.
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http://dx.doi.org/10.3390/ijms22020661DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7827477PMC
January 2021

Duration and quality of sleep during pregnancy are associated with preterm birth and small for gestational age: A prospective study.

Int J Gynaecol Obstet 2021 Jan 9. Epub 2021 Jan 9.

Department of Nutrition, Food Safety and Toxicology, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, China.

Objective: To explore the associations of duration and quality of sleep during pregnancy with preterm birth and small for gestational age (SGA).

Methods: A prospective study was carried out on 1082 healthy women with singleton pregnancies from Chengdu, China. Self-report questionnaires, including duration and quality of sleep and other information, were administered at 8-12, 24-28, and 32-36 weeks of pregnancy. Data on gestational age and weight and length of the neonates were recorded after delivery. After controlling the potential confounders, a multivariable logistic regression model was performed to evaluate whether duration and quality of sleep were associated with preterm birth and SGA.

Results: Participants with short duration of sleep during the third trimester were more likely to report preterm birth (odds ratio [OR] 2.16, 95% confidence interval [CI] 1.26-4.81) and SGA (OR 2.67, 95% CI 1.18-6.54). Participants with poor quality of sleep during the third trimester were at high risk for preterm birth (OR 2.26, 95% CI 1.29-5.84) and SGA (OR 2.08, 95% CI 1.19-5.38).

Conclusion: Short duration and poor quality of sleep during pregnancy are associated with an increased risk of preterm birth and SGA. Sleep characteristics should be assessed during prenatal evaluations to decrease adverse maternal and fetal outcomes.
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http://dx.doi.org/10.1002/ijgo.13584DOI Listing
January 2021

A Regulatory Role of Chemokine Receptor CXCR3 in the Pathogenesis of Chronic Obstructive Pulmonary Disease and Emphysema.

Inflammation 2021 Jan 7. Epub 2021 Jan 7.

Department of Pulmonary and Critical Care Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, #1 Shuaifuyuan, Dongcheng District, Beijing, 100730, China.

Chronic obstructive pulmonary disease (COPD)/pulmonary emphysema is driven by the dysregulated airway inflammation and primarily influenced by the interaction between cigarette smoking (CS) and the individual's susceptibility. The inflammation in COPD involves both innate and adaptive immunity. By binding to its specific ligands, chemokine receptor CXCR3 plays an important role in regulating tissue inflammation and damage. In acute animal model challenged with either CS or pathogens, CXCR3 knockout (KO) attenuated lung inflammation and pathology. However, the role of CXCR3 in CS-induced chronic airway inflammation and pulmonary emphysema remains unknown. In this present study, we investigated the effect of CXCR3 in CS-induced pulmonary emphysema in an animal model, and the association between CXCR3 single nucleotide polymorphisms (SNPs) and COPD susceptibility in human subjects. We found that after chronic exposure to side stream CS (SSCS) for 24 weeks, CXCR3 KO mice demonstrated significant airspace enlargement expressed by mean linear intercept (Lm) compared with the wild-type (WT) mice. Consistently, CXCR3 KO mice had significantly higher BAL fluid macrophages and neutrophils, TNFα, and lung homogenate MMP-9 and MMP-12. Through genetic analysis of CXCR3 polymorphisms in a cohort of COPD patients with Han Chinese ethnicity, one CXCR3 SNP, rs2280964, was found to be genetically related to COPD susceptibility. Furthermore, CXCR3 SNP rs2280964 was significantly associated with the levels of serum MMP-9 in COPD patients. Our data from both animal and human studies revealed a novel role of CXCR3 possibly via influencing MMP9 production in the pathogenesis and progression of CS-associated COPD/pulmonary emphysema.
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http://dx.doi.org/10.1007/s10753-020-01393-9DOI Listing
January 2021

Utilization of circulating cell-free DNA profiling to guide first-line chemotherapy in advanced lung squamous cell carcinoma.

Theranostics 2021 1;11(1):257-267. Epub 2021 Jan 1.

Department of Oncology, Qingdao Municipal Hospital, Qingdao, Shandong, China.

Platinum-based chemotherapy is one of treatment mainstay for patients with advanced lung squamous cell carcinoma (LUSC) but it is still a "one-size fits all" approach. Here, we aimed to investigate the predictive and monitoring role of circulating cell-free DNA (cfDNA) profiling for the outcome of first-line chemotherapy in patients with advanced LUSC. Peripheral blood samples of 155 patients from a phase IV trial and 42 cases from an external real-world cohort were prospectively collected. We generated a copy number variations-based classifier via machine learning algorithm to integrate molecular profiling of cfDNA, named RESPONSE SCORE (RS) to predict the treatment outcome. To monitor the treatment efficacy, cfDNA samples collected at different time points were subjected to an ultra-deep sequencing platform. The results showed that patients with high RS showed substantially higher objective response rate than those with low RS in training set ( < 0.001), validation set ( < 0.001) and real-world cohort ( = 0.019). Furthermore, a significant difference was observed in both progression-free survival (training set, < 0.001; validation set: < 0.001; real-world cohort: = 0.019) and overall survival (training set, < 0.001; validation set: = 0.037) between high and low RS group. Notably, variant allele frequency (VAF) calculated from an ultra-deep sequencing platform significantly reduced in patients experienced a complete or partial response after 2 cycles of chemotherapy ( < 0.001), while it significantly increased in these of non-responder ( < 0.001). Moreover, VAF undetectable after 2 cycles of chemotherapy was correlated with markedly better objective response rate ( < 0.001) and progression-free survival ( < 0.001) than those with detectable VAF. These findings indicated that the RS, a circulating cfDNA sequencing-based stratification index, could help to guide first-line chemotherapy in advanced LUSC. The change of VAF is valuable to monitor the treatment response.
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http://dx.doi.org/10.7150/thno.51243DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7681090PMC
January 2021

Perinatal Depression of Exposed Maternal Women in the COVID-19 Pandemic in Wuhan, China.

Front Psychiatry 2020 16;11:551812. Epub 2020 Dec 16.

Obstetrics Department, Maternal and Child Health Hospital of Hubei Province, Huazhong University of Science and Technology, Wuhan, China.

This study aims to investigate perinatal depression in women who gave birth during the COVID-19 pandemic in Wuhan, and to evaluate the effect of the pandemic on perinatal depression prevalence. A cross-sectional investigation was conducted into women hospitalized for delivery in Hubei Maternity and Child Healthcare Hospital from December 31, 2019 to March 22, 2020, a period which encompasses the entire time frame of the COVID-19 pandemic in Wuhan. The Edinburgh Postnatal Depression Scale (EPDS) was adopted to evaluate perinatal depression status. A Chi-square test and logistic regression model were utilized for data analysis. A total of 2,883 participants were included, 33.71% of whom were found to suffer from depressive symptoms. In detail, 27.02%, 5.24%, and 1.46% were designated as having mild, moderate, and severe depressive symptoms, respectively. The perinatal depression prevalence increased as the COVID-19 pandemic worsened. Compared to the period from December 31, 2019 to January 12, 2020, perinatal depression risk significantly decreased within the 3 weeks of March 2-22, 2020 (1st week: OR = 0.39, 95% CI: 0.20, 0.78; 2nd week: OR = 0.35, 95% CI: 0.17, 0.73; and 3rd week: OR = 0.48, 95% CI: 0.25, 0.94); and the postnatal depression risk significantly rose within the four weeks of January 27-February 23, 2020 (1st week: OR = 1.78, 95% CI: 1.18, 2.68; 2nd week: OR = 2.03, 95% CI: 1.35, 3.04; 3rd week: OR = 1.48, 95% CI: 1.02, 2.14; and 4th week: OR = 1.73, 95% CI: 1.20, 2.48). The dynamic change of perinatal depression was associated with the progression of the COVID-19 pandemic among new mothers who were exposed to the pandemic. An elevated risk of postnatal depression was also observed during the COVID-19 pandemic.
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http://dx.doi.org/10.3389/fpsyt.2020.551812DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7772463PMC
December 2020

[Selenium Threshold for the Delimitation of Natural Selenium-Enriched Land].

Huan Jing Ke Xue 2021 Jan;42(1):333-342

Institute of Geophysical & Geochemical Exploration, Chinese Academy of Geological Sciences, Langfang 065000, China.

The nationwide Se-enriched threshold plays a key role in identifying China's selenium-enriched land resources and developing characteristic agricultural practices. In this study, we used the cooperative data of 10222 sets of crops and roots in China for the past 10 years with a systematic analysis of the selenium content characteristics of the soil and the status of selenium-enriched agricultural products. The preliminary estimates of the selenium-enriched threshold based on a bulk crop-soil linear model and population selenium-intake are presented. Finally, a collaborative analysis model of soil selenium-enrichment rate and crop selenium-enrichment rate is established, coming up with the nationwide Se-enriched threshold:total selenium ≥ 0.40 μg·g in paddy soil, and total selenium ≥ 0.30 μg·g in dryland soil. The threshold passed the feasibility test in 13 provinces, providing strong support for the China Geological Survey to formulate and promulgate this technical standard for the delimitation of the natural selenium-enriched land.
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http://dx.doi.org/10.13227/j.hjkx.202005148DOI Listing
January 2021

Human Stress and StO2: Database, Features, and Classification of Emotional and Physical Stress.

Entropy (Basel) 2020 Aug 31;22(9). Epub 2020 Aug 31.

Chongqing Key Laboratory of Non-Linear Circuit and Intelligent Information Processing, Southwest University, Chongqing 400715, China.

Emotional and physical stress can cause various health problems. In this paper, we used tissue blood oxygen saturation (StO2), a newly proposed physiological signal, to classify the human stress. We firstly constructed a public StO2 database including 42 volunteers subjected to two types of stress. During the physical stress experiment, we observed that the facial StO2 right after the stress can be either increased or decreased comparing to the baseline. We investigated the StO2 feature combinations for the classification and found that the average StO2 values from left cheek, chin, and the middle of the eyebrow can provide the highest classification rate of 95.56%. Comparison with other stress classification method shows that StO2 based method can provide best classification performance with lowest feature dimension. These results suggest that facial StO2 can be used as a promising features to identify stress states, including emotional and physical stress.
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http://dx.doi.org/10.3390/e22090962DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7597254PMC
August 2020

Identification of a quantitative trait loci (QTL) associated with ammonia tolerance in the Pacific white shrimp (Litopenaeus vannamei).

BMC Genomics 2020 Dec 2;21(1):857. Epub 2020 Dec 2.

Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning, 530021, China.

Background: Ammonia is one of the most common toxicological environment factors affecting shrimp health. Although ammonia tolerance in shrimp is closely related to successful industrial production, few genetic studies of this trait are available.

Results: In this study, we constructed a high-density genetic map of the Pacific white shrimp (Litopenaeus vannamei) using specific length amplified fragment sequencing (SLAF-seq). The constructed genetic map contained 17,338 polymorphic markers spanning 44 linkage groups, with a total distance of 6360.12 centimorgans (cM) and an average distance of 0.37 cM. Using this genetic map, we identified a quantitative trait locus (QTL) that explained 7.41-8.46% of the phenotypic variance in L. vannamei survival time under acute ammonia stress. We then sequenced the transcriptomes of the most ammonia-tolerant and the most ammonia-sensitive individuals from each of four genetically distinct L. vannamei families. We found that 7546 genes were differentially expressed between the ammonia-tolerant and ammonia-sensitive individuals. Using QTL analysis and the transcriptomes, we identified one candidate gene (annotated as an ATP synthase g subunit) associated with ammonia tolerance.

Conclusions: In this study, we constructed a high-density genetic map of L. vannamei and identified a QTL for ammonia tolerance. By combining QTL and transcriptome analyses, we identified a candidate gene associated with ammonia tolerance. Our work provides the basis for future genetic studies focused on molecular marker-assisted selective breeding.
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http://dx.doi.org/10.1186/s12864-020-07254-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7709431PMC
December 2020

Effect of magnesium sulfate combined with labetalol on serum sFlt-1/PlGF ratio in patients with early-onset severe pre-eclampsia.

Exp Ther Med 2020 Dec 27;20(6):276. Epub 2020 Oct 27.

Department of Obstetrics, Maternity and Child Health Care Hospital of Hubei, Wuhan, Hubei 430000, P.R. China.

The aim of the present study was to investigate the therapeutic effect of magnesium sulfate combined with labetalol on the early-onset severe pre-eclampsia (ES-PE) and explore the role of soluble fms-like tyrosine kinase-1 (sFlT-1), placental growth factor (PlGF), and sFlt-1/PlGF ratio in the treatment. A total of 164 ES-PE patients admitted to the Maternity and Child Health Care Hospital of Hubei (Wuhan, China) were assigned to this observational study. Among them, 83 patients were enrolled in group A and treated with magnesium sulfate combined with labetalol hydrochloride, and 81 patients were enrolled in group B and treated with magnesium sulfate. The therapeutic effect, adverse reactions and pregnancy outcomes in the two groups were analyzed. Serum sFlt-1 and PlGF concentrations, before and after treatment, were measured by enzyme-linked immunosorbent assay (ELISA). Receiver operating characteristic (ROC) curve analysis was performed to assess the predictive value of pre-treatment serum sFlt-1/PlGF ratio for the clinical outcome. The effective rate was significantly higher in group A than that in group B. Group A presented superior pregnancy outcomes over group B. The serum sFlt-1 concentration and sFlt-1/PlGF ratio after treatment were significantly lower than those before treatment in groups A and B, whereas PlGF concentration was significantly higher after treatment in both groups. After treatment, group A had markedly lower serum sFlt-1 concentration and sFlt-1/PlGF ratio than group B, and markedly higher PlGF concentration than group B. The area under curve (AUC) of serum sFlt-1/PlGF ratio before treatment for the prediction of the clinical efficacy was 0.737. In conclusion, magnesium sulfate combined with labetalol could be effectively used for the treatment of ES-PE. The results of ELISA revealed that the balance of sFlT-1 and PlGF was improved after treatment and the sFlT-1/PlGF ratio was decreased. The assessment of sFlt-1/PlGF ratio before treatment was shown to have a certain predictive value for the efficacy of ES-PE treatment.
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http://dx.doi.org/10.3892/etm.2020.9406DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7664615PMC
December 2020

Construction of a High-Density Genetic Map and Identification of Quantitative Trait Loci for Nitrite Tolerance in the Pacific White Shrimp ().

Front Genet 2020 24;11:571880. Epub 2020 Sep 24.

Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning, China.

Nitrite is a major environmental toxin in aquaculture systems that disrupts multiple physiological functions in aquatic animals. Although nitrite tolerance in shrimp is closely related to successful industrial production, few genetic studies of this trait are available. In this study, we constructed a high-density genetic map of with 17,242 single nucleotide polymorphism markers spanning 6,828.06 centimorgans (cM), with an average distance of 0.4 cM between adjacent markers on 44 linkage groups (LGs). Using this genetic map, we identified two markers associated with nitrite tolerance. We then sequenced the transcriptomes of the most nitrite-tolerant and nitrite-sensitive individuals from each of four genetically distinct families (LV-I-4). We found 2,002, 1,983, 1,954, and 1,867 differentially expressed genes in families LV-1, LV-2, LV-3, and LV-4, respectively. By integrating QTL and transcriptomics analyses, we identified a candidate gene associated with nitrite tolerance. This gene was annotated as solute carrier family 26 member 6 (). RNA interference (RNAi) analysis demonstrated that was critical for nitrite tolerance in . The present study increases our understanding of the molecular mechanisms underlying nitrite tolerance in shrimp and provides a basis for molecular-marker-assisted shrimp breeding.
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http://dx.doi.org/10.3389/fgene.2020.571880DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7541944PMC
September 2020

Replication Gaps Underlie BRCA Deficiency and Therapy Response.

Cancer Res 2021 03 12;81(5):1388-1397. Epub 2020 Nov 12.

University of Massachusetts Medical School, Worcester, Massachusetts.

Defects in DNA repair and the protection of stalled DNA replication forks are thought to underlie the chemosensitivity of tumors deficient in the hereditary breast cancer genes and (BRCA). Challenging this assumption are recent findings that indicate chemotherapies, such as cisplatin used to treat BRCA-deficient tumors, do not initially cause DNA double-strand breaks (DSB). Here, we show that ssDNA replication gaps underlie the hypersensitivity of BRCA-deficient cancer and that defects in homologous recombination (HR) or fork protection (FP) do not. In BRCA-deficient cells, ssDNA gaps developed because replication was not effectively restrained in response to stress. Gap suppression by either restoration of fork restraint or gap filling conferred therapy resistance in tissue culture and BRCA patient tumors. In contrast, restored FP and HR could be uncoupled from therapy resistance when gaps were present. Moreover, DSBs were not detected after therapy when apoptosis was inhibited, supporting a framework in which DSBs are not directly induced by genotoxic agents, but rather are induced from cell death nucleases and are not fundamental to the mechanism of action of genotoxic agents. Together, these data indicate that ssDNA replication gaps underlie the BRCA cancer phenotype, "BRCAness," and we propose they are fundamental to the mechanism of action of genotoxic chemotherapies. SIGNIFICANCE: This study suggests that ssDNA replication gaps are fundamental to the toxicity of genotoxic agents and underlie the BRCA-cancer phenotype "BRCAness," yielding promising biomarkers, targets, and opportunities to resensitize refractory disease..
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http://dx.doi.org/10.1158/0008-5472.CAN-20-1602DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8026497PMC
March 2021

Determinants of Maternal Behavior of Mobile Phone Use during Pregnancy.

J Healthc Eng 2020 23;2020:9465019. Epub 2020 Oct 23.

Department of Obstetrics, Maternal and Child Health Hospital of Hubei Province, Wuhan, China.

Excessive use of mobile phones might bring negative physical and psychological consequences to pregnant women. This study aims to explore the potential determinants of pregnant women's mobile phone use behavior to assist healthcare providers in the development of guideline programs. In order to explain the behavior, we developed a theoretical model based on the widely applied theory of planned behavior (TPB) by incorporating two additional constructs of personal habit and perceived risk. Structural equation modeling technique is employed to estimate the model based on questionnaire survey. Research results clearly show that behavior attitude and perceived behavior control play dominant roles in determining the intention and behavior. It is interesting to find that perceived risk and personal habit are less important in determining pregnant women's behavior of mobile phone use. Finally, suggestions are put forward to reduce the risk of mobile phone use during pregnancy.
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http://dx.doi.org/10.1155/2020/9465019DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7603607PMC
October 2020

[Ecological Risk Assessment of Heavy Metals at Township Scale in the High Background of Heavy Metals, Southwestern, China].

Huan Jing Ke Xue 2020 Sep;41(9):4197-4209

Research Center of Geochemical Survey and Assessment on Land Quality, China Geological Survey, Langfang 065000, China.

Heavy metals (HMs) are naturally occurring elements that have high natural background levels in the environment. Therefore, it is important to conduct ecological risk assessment and identify potential sources of HMs. In the past, studies were conducted at the regional scale. The accuracy of those studies could not meet the needs of spatial planning and natural resource management. Therefore, it is necessary to conduct ecological risk assessment at the township scale. In this study, 1092 soil samples (from 0-20 cm depth) were collected in the town of Reshui, an area with high background levels of soil HMs with the parent material of carbonatite, which is commonly found in Southwest China. The town of Reshui is a multi-ecological risk superimposed area where the ecological risk is high. In this study, concentrations of HMs (Cd, Cr, As, Hg, Pb, Cu, Zn, and Ni) in the topsoil were analyzed, and statistical analysis (SA), geographic information system (GIS) modeling, and positive matrix factorization (PMF) analysis were performed. The geoaccumulation index () and potential ecological risk index (PERI) were applied for the ecological risk assessment and quantification of the sources of the soil HMs. The mean values of HM concentrations in the topsoil were 18.1, 1.18, 174.1, 202.2, 0.09, 71.1, 34.9, and 167.2 mg ·kgfor As, Cd, Cr, Cu, Hg, Ni, Pb, and Zn, respectively, which were considerably higher than the average background value (ABV) in soils in Yunnan Province except for As and Pb. The average concentrations of Cd, Cr, Cu, and Ni exceeded the screening values specified in the soil contamination risk in agricultural land (GB 15618-2018) by 5.82, 1.16, 4.04, and 1.02 times, respectively. The value shows that the major pollutant is Cu in the surface soil of the study area, followed by Cr, and Cd. Speciation analysis of HMs indicates that HMs (Cr, As, Pb, Cu, Zn, and Ni) mainly exist in the residual form, mostly from the geological background with low bioavailability. The potential effective components of Hg have higher levels, but the total amount of Hg and its pollution risk are lower. Cd has a high bioavailability ratio, is easy to enter the soil solution and be absorbed by crops, and is the HM with the highest pollution risk in the study area. The PERI shows that the proportions of low ecological risk, moderate risk, and high risk soil samples are 44.23%, 54.40%, and 1.37% of the total number of samples, respectively. Hg and Cd were the major sources of risk because of their high toxicity coefficient. The PMF analysis indicates that there are four major sources of HMs in the study area: human activity, natural sources, coal mining and traffic emissions, and agricultural sources with the risk contribution ratios of 9.29%, 53.67%, 11.23%, and 25.81%, respectively. The PMF analysis effectively quantified the ecological risk from these sources, providing a reference for further pollution control and prevention measures.
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http://dx.doi.org/10.13227/j.hjkx.201912241DOI Listing
September 2020

[Discussion on Key Issues of Geochemical Monitoring of Soil Heavy Metal in Xiong'an New District].

Huan Jing Ke Xue 2020 Sep;41(9):4169-4179

Institute of Geophysical&Geochemical Exploration, Chinese Academy of Geological Sciences, Langfang 065000, China.

To build a soil environmental quality monitoring network and collect the systematic soil geochemical data of heavy metals before and after the construction of Xiong'an New District(XND), long-term variations in soil heavy metal levels during the XND construction were monitored based on the land quality geochemical survey monitoring data and the geochemical distribution of heavy metals. Parameters such as sampling density, sampling quantity, and sampling period were estimated to construct the geochemical monitoring network, which provides a reference for the future long-term monitoring of soil heavy metal geochemistry in XND. The results indicate that variations in the amount of heavy metals As, Cd, Cu, Hg, and Pb are greater than 36%, while the nugget coefficients of Cd, Cu, and Pb are greater than 75% because of the superposition of manmade sources on the high natural background levels. At the 95% and 90% confidence levels, while the allowable error of the sample mean value is 5%, 10%, and 15% and heavy metals as As, Cd, Cu, Hg,Ni, Pb and Zn is monitored in soil environmental quality monitoring network, the proper numbers in the study area are 1077, 270, 120 and 767, 192, 86, respectively. The minimum detectable change(MDC)of each heavy metal species is between 0.0025 and 2.54 mg ·kg. Soil heavy metal monitoring requires different revisit intervals under different annual change rates, but in the study area, revisit intervals of soil monitoring are approximately two years under the current conditions of constant annual change rates.
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http://dx.doi.org/10.13227/j.hjkx.202002134DOI Listing
September 2020