Publications by authors named "Min Lu"

1,028 Publications

  • Page 1 of 1

Development and Comparison of Predictive Models Based on Different Types of Influencing Factors to Select the Best One for the Prediction of OSAHS Prevalence.

Front Psychiatry 2022 6;13:892737. Epub 2022 Jul 6.

Department of Emergency, Shangrao Hospital Affiliated to Nanchang University, Shangrao People's Hospital, Shangrao, China.

Objective: This study aims to retrospectively analyze numerous related clinical data to identify three types of potential influencing factors of obstructive sleep apnea-hypopnea syndrome (OSAHS) for establishing three predictive nomograms, respectively. The best performing one was screened to guide further clinical decision-making.

Methods: Correlation, difference and univariate logistic regression analysis were used to identify the influencing factors of OSAHS. Then these factors are divided into three different types according to the characteristics of the data. Lasso regression was used to filter out three types of factors to construct three nomograms, respectively. Compare the performance of the three nomograms evaluated by C-index, ROC curve and Decision Curve Analysis to select the best one. Two queues were obtained by randomly splitting the whole queue, and similar methods are used to verify the performance of the best nomogram.

Results: In total, 8 influencing factors of OSAHS have been identified and divided into three types. Lasso regression finally determined 6, 3 and 4 factors to construct mixed factors nomogram (MFN), baseline factors nomogram (BAFN) and blood factors nomogram (BLFN), respectively. MFN performed best among the three and also performed well in multiple queues.

Conclusion: Compared with BAFN and BLFN constructed by single-type factors, MFN constructed by six mixed-type factors shows better performance in predicting the risk of OSAHS.
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http://dx.doi.org/10.3389/fpsyt.2022.892737DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9340571PMC
July 2022

Artificially steering electrocatalytic oxygen evolution reaction mechanism by regulating oxygen defect contents in perovskites.

Sci Adv 2022 Jul 29;8(30):eabq3563. Epub 2022 Jul 29.

State Key Laboratory of Applied Organic Chemistry, Frontiers Science Center for Rare Isotopes, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China.

The regulation of mechanism on the electrocatalysis process with multiple reaction pathways is more efficient and essential than conventional material engineering for the enhancement of catalyst performance. Here, by using oxygen evolution reaction (OER) as a model, which has an adsorbate evolution mechanism (AEM) and a lattice oxygen oxidation mechanism (LOM), we demonstrate a general strategy for steering the two mechanisms on various LaSrCoO. By delicately controlling the oxygen defect contents, the dominant OER mechanism on LaSrCoO can be arbitrarily transformed between AEM-LOM-AEM accompanied by a volcano-type activity variation trend. Experimental and computational evidence explicitly reveal that the phenomenon is due to the fact that the increased oxygen defects alter the lattice oxygen activity with a volcano-type trend and preserve the Co state for preferably OER. Therefore, we achieve the co-optimization between the activity and stability of catalysts by altering the mechanism rather than a specific design of catalysts.
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http://dx.doi.org/10.1126/sciadv.abq3563DOI Listing
July 2022

Preparation of protamine-hyaluronic acid coated core-shell nanoparticles for enhanced solubility, permeability, and oral bioavailability of decoquinate.

Int J Biol Macromol 2022 Jul 22;218:346-355. Epub 2022 Jul 22.

National Engineering Research Center for Process Development of Active Pharmaceutical Ingredients, Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Zhejiang University of Technology, Hangzhou 310014, PR China; Key Laboratory for Green Pharmaceutical Technologies and Related Equipment of Ministry of Education, College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310014, PR China. Electronic address:

Decoquinate (DQ) has low oral bioavailability owing to its poor water solubility. In this study, a DQ solid dispersion (DQ-SD) was fabricated using mechanochemical technology to encapsulate DQ and improve its oral bioavailability. DQ-SD is easily generated via self-assembly in the aqueous phase to form micelles consisting of disodium glycyrrhizinate (NaGA) nanoparticles with a protamine (PRM) and anionic hyaluronic acid (HA) layers. The spherical DQ nanoparticles with an average diameter of 114.95 nm were obtained in an aqueous phase with a critical micelle concentration of 0.157 mg/mL, zeta potential of -38.38 mV, polydispersity index of 0.200, and drug loading of 5.66 %. The dissolution rate and cumulative release of DQ-SD were higher than those of pure DQ. Furthermore, the bioavailability of DQ-SD was approximately 6.3 times higher than that of pure DQ. Pharmacokinetic and biodistribution studies indicated that DQ-SD possessed a significantly higher concentration in the blood and preferential liver tissue accumulation, than that of pure DQ. The developed DQ-SD exhibited considerable potential for developing old DQ for a new application as a hematogenous parasite drug and provides a reference for developing more efficient delivery systems for hydrophobic bioactive agents.
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http://dx.doi.org/10.1016/j.ijbiomac.2022.07.152DOI Listing
July 2022

FGFR1 promotes tumor immune evasion via YAP-mediated PD-L1 expression upregulation in lung squamous cell carcinoma.

Cell Immunol 2022 Jul 9;379:104577. Epub 2022 Jul 9.

Shanghai Lung Cancer Center, Shanghai Chest Hospital, Shanghai Jiao Tong University, West Huaihai Road 241, Shanghai 200030, China. Electronic address:

Background: Variations in FGFR1 are common driver mutations of LSQCC. And immune checkpoint inhibitors targeting PD-1 and PD-L1 are powerful anticancer weapons. Activation of FGFR1 leads to tumorigenesis through multiple downstream molecules, including YAP, but whether and how FGFR1 regulates tumor immune evasion remain largely unclear.

Methods: LSQCC cells were modified to increase or decrease the expression of FGFR1, YAP and PD-L1, as assessed by molecular assays. After FGFR1 knockdown, cancer cells were assessed after cocultured with Jurkat T cells in vitro, and the tumor microenvironment were analyzed in C57BL/6 mice. The effect of the combination of FGFR1 knockdown and PD-1 blockade was also explored.

Results: In human LSQCC, activation of FGFR1 was positively correlated with transcription of PD-L1. In H520 and HCC95 cells, FGFR1 upregulated PD-L1 expression via YAP, and YAP initiated the transcription of PD-L1 after binding to its promoter region. FGFR1 knockdown decreased tumor growth, reduced immune escape and induced reactivation of CD8+ T cells. The combination of FGFR1 knockdown and PD-1 blockade synergistically exerted antitumor effects.

Conclusions: The FGFR1/YAP/PD-L1 regulatory axis mediates tumor-associated immune suppression in lung squamous cell carcinoma, and FGFR1 knockdown reactivates T cells in the tumor microenvironment. Synergistic inhibition of both FGFR1 and PD-1/PD-L1 pathways may be a possible treatment for lung cancer patients.
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http://dx.doi.org/10.1016/j.cellimm.2022.104577DOI Listing
July 2022

Concordant and Heterogeneity of Single-Cell Transcriptome in Cardiac Development of Human and Mouse.

Front Genet 2022 27;13:892766. Epub 2022 Jun 27.

Key Laboratory of Arrhythmias, Ministry of Education of China, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.

Normal heart development is vital for maintaining its function, and the development process is involved in complex interactions between different cell lineages. How mammalian hearts develop differently is still not fully understood. In this study, we identified several major types of cardiac cells, including cardiomyocytes (CMs), fibroblasts (FBs), endothelial cells (ECs), ECs/FBs, epicardial cells (EPs), and immune cells (macrophage/monocyte cluster, MACs/MONOs), based on single-cell transcriptome data from embryonic hearts of both human and mouse. Then, species-shared and species-specific marker genes were determined in the same cell type between the two species, and the genes with consistent and different expression patterns were also selected by constructing the developmental trajectories. Through a comparison of the development stage similarity of CMs, FBs, and ECs/FBs between humans and mice, it is revealed that CMs at e9.5 and e10.5 of mice are most similar to those of humans at 7 W and 9 W, respectively. Mouse FBs at e10.5, e13.5, and e14.5 are correspondingly more like the same human cells at 6, 7, and 9 W. Moreover, the e9.5-ECs/FBs of mice are most similar to that of humans at 10W. These results provide a resource for understudying cardiac cell types and the crucial markers able to trace developmental trajectories among the species, which is beneficial for finding suitable mouse models to detect human cardiac physiology and related diseases.
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http://dx.doi.org/10.3389/fgene.2022.892766DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9271823PMC
June 2022

A functional reference map of the RNF8 interactome in cancer.

Biol Direct 2022 07 13;17(1):17. Epub 2022 Jul 13.

Department of Biology and Chemistry, College of Sciences, National University of Defense Technology, Changsha, 410073, Hunan, China.

Background: RNF8 is an E3 ligase identified as a critical DNA damage-responsive protein. Recently, multiple reports have shown that RNF8 could be used as an important therapeutic target for cancer chemo/radiotherapy. However, the understanding of RNF8 remains limited due to the lack of its interactome reference map and comprehensive analysis of RNF8 in diverse cancers, which underscores the need to map the interactome of RNF8 via high-throughput methods.

Results: A two-way identification method based on LC-MS was designed for the identification of the RNF8 interactome with high-specificity. By in silico analysis and in vitro validation, we identified a new reference map of the RNF8 interactome network containing many new targets, such as YBX1, DNMT1, and HDCA1, new biological functions and the gene-disease associations of RNF8. Our results revealed a close relationship between RNF8 and neurodegenerative diseases or tumor-infiltrating immune cells using bulk RNA-seq and scRNA-seq datasets. As a proof of concept of our interactome map, we validated the direct binding between RNF8 and YBX1 and showed that RNF8 catalyzed the ubiquitination of YBX1. These results demonstrated that RNF8 might be a crucial regulator of YBX1.

Conclusions: Our work provides a unique framework for researchers and clinicians who seek to better explore or understand RNF8-regulated biological functions in cancers. This study will hopefully facilitate the rational design and further development of anti-RNF8 therapy in cancers.
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http://dx.doi.org/10.1186/s13062-022-00331-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9277853PMC
July 2022

Effects of oral administration and intravenous injection of polygalacturonic acid on the immunomodulation and gut microbiota in UC mice.

Int J Biol Macromol 2022 Jul 10;217:150-160. Epub 2022 Jul 10.

Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, Jiangsu, PR China. Electronic address:

This study aimed to compare the differences between oral administration and intravenous injection of polygalacturonic acid (PGA) in the regulation of immune and intestinal microflora in ulcerative colitis (UC) mice. PGA was administered orally or intravenously. PGA in the high-dose ig group was the most effective in treating UC by increasing colon length and downregulating disease activity index, histopathological score and proinflammatory cytokine levels. In spleen, the efficacy of PGA on restoring Th17/Treg balance in the high-dose iv group was better than that in the high-dose ig group, the opposite was observed in the lamina propria. The level of colonic IL-17A in the high-dose ig group was lower than that in the high-dose iv group, the opposite was observed for that of colonic IL-10. Western blot and immunohistochemistry analysis revealed that PGA in the high-dose ig group decreased the protein expression of RORγt, and increased that of FOXP3. Furthermore, PGA in the high-dose ig group was more effective than that in the high-dose iv group in improving the intestinal microflora structure. Our results suggest that in immune regulation, oral PGA is more effective in the lamina propria and gut microbiota while intravenous PGA is more effective in the spleen.
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http://dx.doi.org/10.1016/j.ijbiomac.2022.07.039DOI Listing
July 2022

Clinical experience and treatment strategy of leiomyosarcoma originating from the renal vein.

Eur J Med Res 2022 Jun 27;27(1):100. Epub 2022 Jun 27.

Department of Urology, Peking University Third Hospital, Haidian District, Beijing, 100191, People's Republic of China.

Background: Leiomyosarcoma originating from the renal vein (RVLMS) is extremely rare. RVLMS lacks specific clinical manifestations and specific imaging features. This article discusses the epidemiological characteristics and diagnostic difficulties of RVLMS, as well as imaging features, differential diagnosis, treatment strategy, and prognostic factors of this disease.

Method: A case of RVLMS at our center, and 55 cases from the literature based on the PubMed search.

Results: Total operation time was 224 min, and total blood loss during the surgery was 200 ml. Resected tumor was irregular in shape, with negative margins. On the 6th day after the operation, the drainage tube was removed, and the patient was discharged from the hospital. Postoperative pathological results confirmed the renal vein leiomyosarcoma: spindle cell sarcoma, diffuse severe atypia, S-100 (-), SMA ( +), desmin ( +), CD34 (-), CD99 ( +).  Twenty-seven months after the surgery, the patient is alive, and without local recurrence or distant metastases.

Conclusion: Unspecific clinical manifestations and imaging features make the diagnosis of RVLMS difficult. Most patients are diagnosed intra-operatively or following postoperative pathology. Differential diagnosis with paraganglioma (PG) and retroperitoneal sarcoma (RPS) should be made. Early and complete resection is considered as the first choice of treatment, and whether to preserve the kidney is based on the patient's condition. RVLMS is highly malignant, and may recur locally or metastasize to distant locations; therefore, adjuvant therapy and regular follow-up should be carried out after surgery.
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http://dx.doi.org/10.1186/s40001-022-00721-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9235132PMC
June 2022

Comprehensive Analysis of hsa-miR-654-5p's Tumor-Suppressing Functions.

Int J Mol Sci 2022 Jun 8;23(12). Epub 2022 Jun 8.

Department of Biology and Chemistry, College of Liberal Arts and Sciences, National University of Defense Technology, Changsha 410073, China.

The pivotal roles of miRNAs in carcinogenesis, metastasis, and prognosis have been demonstrated recently in various cancers. This study intended to investigate the specific roles of hsa-miR-654-5p in lung cancer, which is, in general, rarely discussed. A series of closed-loop bioinformatic functional analyses were integrated with in vitro experimental validation to explore the overall biological functions and pan-cancer regulation pattern of miR-654-5p. We found that miR-654-5p abundance was significantly elevated in LUAD tissues and correlated with patients' survival. A total of 275 potential targets of miR-654-5p were then identified and the miR-654-5p- regulation axis was validated in vitro as a proof of concept. Furthermore, we revealed the tumor-suppressing roles of miR-654-5p and demonstrated that miR-654-5p inhibited the lung cancer cell epithelial-mesenchymal transition (EMT) process, cell proliferation, and migration using target-based, abundance-based, and ssGSEA-based bioinformatic methods and in vitro validation. Following the construction of a protein-protein interaction network, 11 highly interconnected hub genes were identified and a five-genes risk scoring model was developed to assess their potential prognostic ability. Our study does not only provide a basic miRNA-mRNA-phenotypes reference map for understanding the function of miR-654-5p in different cancers but also reveals the tumor-suppressing roles and prognostic values of miR-654-5p.
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http://dx.doi.org/10.3390/ijms23126411DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9224266PMC
June 2022

Surface Activation and Ni-S Stabilization in NiO/NiS for Efficient Oxygen Evolution Reaction.

Angew Chem Int Ed Engl 2022 Jun 22:e202207217. Epub 2022 Jun 22.

State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, Frontiers Science Center for Rare Isotopes, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, 730000, China.

Manipulating the active species and improving the structural stabilization of sulfur-containing catalysts during the OER process remain a tremendous challenge. Herein, we constructed NiO/NiS and Fe-NiO/NiS as catalyst models to study the effect of Fe doping. As expected, Fe-NiO/NiS exhibits a low overpotential of 270 mV at 10 mA cm . The accumulation of hydroxyl groups on the surface of materials after Fe doping can promote the formation of highly active NiOOH at a lower OER potential. Moreover, we investigated the level of corrosion of M-S bonds and compared the stability variation of M-S bonds with Fe at different locations. Interestingly, Fe bonded with S in the bulk as the sacrificial agent can alleviate the oxidation corrosion of partial Ni-S bonds and thus endow Fe-NiO/NiS long-term durability. This work could motivate the community to focus more on resolving the corrosion of sulfur-containing materials.
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http://dx.doi.org/10.1002/anie.202207217DOI Listing
June 2022

Effect of Bairui Granule on Inflammatory Mediators in Induced Sputum, Leukotriene C4, and EOS in Peripheral Blood of Children with Cough Variant Asthma.

Comput Math Methods Med 2022 8;2022:2657994. Epub 2022 Jun 8.

Department of Paediatrics, The Affiliated Suzhou Science &Technology Town Hospital of Nanjing Medical University, China.

Objective: To study the effect of Hanchuan Zupa granule combined with conventional western medicine in the treatment of children with bronchial asthma.

Methods: 98 cases in Fengrun District People's Hospital of Tangshan City from June 2018 to February 2021 were selected. The control group was given oxygen therapy, antibiotics, and aerosol inhalation of quick acting 2 receptor agonist, glucocorticoid, and other conventional western medicine treatment, while the observation group was treated with Bairui granule on the basis of the control group. The course of treatment of the two groups was 1 week.

Results: After treatment, the levels of sputum IL-4, IL-17, neu, and ECP in the two groups decreased, and the observation group was lower than the control group ( < 0.05). The levels of EOS, CXCR4, LTB4, and SDF-1 in peripheral blood of the two groups were lower than those in the control group ( < 0.05). The daytime cough, night cough, and TCM syndrome scores of the two groups were decreased, and the observation group was lower than the control group ( < 0.05).

Conclusion: Bairui granule combined with conventional western medicine in the treatment of children with bronchial asthma, the curative effect is worthy of affirmation, can effectively improve cough symptoms, reduce EOS, CXCR, LTB4, SDF-1 levels, inhibit airway inflammation, and has good clinical application value.
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http://dx.doi.org/10.1155/2022/2657994DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9200585PMC
June 2022

The Effect of Comprehensive Use of PDCA and FMEA Management Tools on the Work Efficiency, Teamwork, and Self-Identity of Medical Staff: A Cohort Study with Zhongda Hospital in China as an Example.

Contrast Media Mol Imaging 2022 23;2022:5286062. Epub 2022 May 23.

Medical Insurance Office, Zhongda Hospital, Southeast University, Nanjing 210009, China.

Objective: Taking Zhongda Hospital in China as an example, this study discusses the impact of comprehensive use of PDCA and FMEA management tools on the work efficiency, teamwork, and self-identity of medical staff.

Methods: Two hundred medical staff in our hospital from January 2020 to December 2021 were selected as the research subjects, and the 200 medical staff were divided into a control group and a research group by the digital table method, with 100 cases in each group. The medical staff in the control group implemented conventional system management methods, while the research group comprehensively used PDCA and FMEA management tools based on implementing conventional system management. The differences in work efficiency, teamwork, and self-identity of medical staff were compared.

Results: Before the study, there exhibited no significant difference in the work efficiency, teamwork, and self-identity scores of medical staff ( > 0.05). After an intervention, the work efficiency, teamwork, and self-identity scores of medical staff in the study group were higher than those in the control group ( < 0.05). After the intervention, the management quality score of the research group was higher than that of the control group ( < 0.05); after the intervention, the medical staff in the research group had lower work efficiency, insufficient professional ability, and insufficient management system cognitive behaviours than that in the control group ( < 0.05).

Conclusion: The comprehensive use of PDCA and FMEA management tools in internal hospital management can remarkably enhance the work efficiency, teamwork, and self-identity of medical staff.
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http://dx.doi.org/10.1155/2022/5286062DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9162864PMC
June 2022

Metabolomics Combined with Transcriptomics Analysis Revealed the Amino Acids, Phenolic Acids, and Flavonol Derivatives Biosynthesis Network in Developing Fruit.

Foods 2022 Jun 1;11(11). Epub 2022 Jun 1.

National Forestry and Grassland Administration Engineering Research Center for Rosa roxburghii, Guiyang 550025, China.

Tratt. is a specific fruit with high nutritional value and antioxidative activities. However, the key metabolites and their biosynthesis are still unknown. Herein, a main cultivated variety, 'Guinong 5' (Rr5), was chosen to analyze the metabolomics of the three developmental stages of fruit by liquid chromatography-tandem mass spectrometry (LC-MS/MS). A total of 533 metabolites were identified, of which 339 were significantly altered. Total phenols, flavonoids, and amino acids were significantly correlated to at least one in vitro antioxidant activity. The conjoint Kyoto Encyclopedia of Genes and Genomes (KEGG) co-enrichment analysis of metabolome and transcriptome was focused on amino acid, phenylpropanoid, and flavonoid biosynthesis pathways. The amino acid, phenolic acid, and flavonol biosynthesis networks were constructed with 32 structural genes, 48 , and 23 metabolites. Of these, six correlated to 9-15 metabolites in the network were selected to detect the gene expression in six different genotypes fruits. Subsequently, 21 key metabolites were identified in the in vitro antioxidant activities in the fruits at various developmental stages or in fruits of different genotypes. We found that four key were related to the significantly varied amino acids, phenolic acids, and flavonol derivatives in the network during fruit development and the key metabolites in the in vitro antioxidative activities in the fruits of six genotypes. This finding provided novel insights into the flavonoid, polyphenol, and amino acid synthesis in .
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http://dx.doi.org/10.3390/foods11111639DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9180193PMC
June 2022

Novel Dual-Color Immunochromatographic Assay Based on Chrysanthemum-like [email protected] and Colloidal Gold for Simultaneous Sensitive Detection of Paclobutrazol and Carbofuran in Fruits and Vegetables.

Foods 2022 May 26;11(11). Epub 2022 May 26.

State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.

To ensure food safety and prevent the toxic effects of paclobutrazol (PBZ) and carbofuran (CAR) on humans, a sensitive and rapid method for the detection of PBZ and CAR in fruits and vegetables is required. Herein, a highly sensitive PBZ monoclonal antibody (PBZ mAb) and CAR monoclonal antibody (CAR mAb) with half-inhibitory concentrations (IC) at 0.77 and 0.82 ng mL were prepared, respectively. We proposed a novel dual-color immunochromatographic assay (ICA) with two test lines (T and T) and an independent control line (C) based on chrysanthemum-like [email protected] ([email protected]) and colloidal gold (AuNPs) for the simultaneous and sensitive detection of PBZ and CAR with naked-eye detection limits of 10 and 5 μg kg, respectively. The limits of detection (LOD) for PBZ and CAR were 0.117 and 0.087 μg kg in orange, 0.109 and 0.056 μg kg in grape, and 0.131 and 0.094 μg kg in cabbage mustard, respectively. The average recoveries of PBZ and CAR in orange, grape, and cabbage mustard were 97.86-102.83%, with coefficients of variation from 8.94 to 11.05. The detection results of this method for 30 samples (orange, grapes, and cabbage mustard) agreed well with those of liquid chromatography-tandem mass spectrometry. The novel dual-color ICA was sensitive, rapid, and accurate for the simultaneous detection of PBZ and CAR in real samples.
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http://dx.doi.org/10.3390/foods11111564DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9180898PMC
May 2022

Molecular Detection of Ofloxacin and Kanamycin Resistance in Patients with MDR and Non-MDR TB from Suburban Districts in Hangzhou, China, 2019-2020.

Biomed Environ Sci 2022 05;35(5):468-471

Department of TB Control and Prevention, Hangzhou Center for Disease Control and Prevention, Hangzhou 310021, Zhejiang, China.

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http://dx.doi.org/10.3967/bes2022.064DOI Listing
May 2022

P2RY2-AKT activation is a therapeutically actionable consequence of XPO1 inhibition in acute myeloid leukemia.

Nat Cancer 2022 Jul 6;3(7):837-851. Epub 2022 Jun 6.

Université de Paris, Génomes, Biologie Cellulaire et Thérapeutique U944, INSERM, CNRS, Paris, France.

Selinexor is a first-in-class inhibitor of the nuclear exportin XPO1 that was recently approved by the US Food and Drug Administration for the treatment of multiple myeloma and diffuse large B-cell lymphoma. In relapsed/refractory acute myeloid leukemia (AML), selinexor has shown promising activity, suggesting that selinexor-based combination therapies may have clinical potential. Here, motivated by the hypothesis that selinexor's nuclear sequestration of diverse substrates imposes pleiotropic fitness effects on AML cells, we systematically catalog the pro- and anti-fitness consequences of selinexor treatment. We discover that selinexor activates PI3Kγ-dependent AKT signaling in AML by upregulating the purinergic receptor P2RY2. Inhibiting this axis potentiates the anti-leukemic effects of selinexor in AML cell lines, patient-derived primary cultures and multiple mouse models of AML. In a syngeneic, MLL-AF9-driven mouse model of AML, treatment with selinexor and ipatasertib outperforms both standard-of-care chemotherapy and chemotherapy with selinexor. Together, these findings establish drug-induced P2RY2-AKT signaling as an actionable consequence of XPO1 inhibition in AML.
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http://dx.doi.org/10.1038/s43018-022-00394-xDOI Listing
July 2022

Identification of heavy metal leaching patterns in municipal solid waste incineration fly ash based on an explainable machine learning approach.

J Environ Manage 2022 Sep 30;317:115387. Epub 2022 May 30.

College of Environmental & Resource Sciences, Zhejiang University, Hangzhou, 310058, China; Chinese Academy of Environmental Planning, Beijing, 100012, China. Electronic address:

The leaching risk of heavy metal (HM) in municipal solid waste incineration fly ash (MSWI-FA) leads to a big challenge for FA landfilling. In this work, the HM leaching patterns were identified via 6 highly available indices of FA, in which 160 stabilized FA samples were collected from 4 incineration plants in eastern China and an explainable machine learning approach based on boosting and game analysis was conducted to assess the leaching potentials of 6 HMs (Cr, Cd, Cu, Ni, Pb and Zn). We found that, there remained high exceeding risks of Cd and Pb in stabilized FA. In addition, the S-Cl (soluble chlorine) content was the most influential factor of the leaching behaviors of Cd, Cu, Pb and Zn, more important than pH in regard to Cu, Pb and Zn. We quantified the influence of S-Cl on the HM leaching of Cd, Cu, Pb and Zn, whereby their leaching concentrations would increase by 223.5%, 215.2%, 216.5% and 222.6%, respectively, for every 0.5 mol/L order increase in S-Cl concentration. Finally, a fast determination criterion for the FA landfill was proposed, that is, FA of which S-Cl was less than 0.412 mol/L was acceptable.
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http://dx.doi.org/10.1016/j.jenvman.2022.115387DOI Listing
September 2022

The Bioinformatical Identification of Potential Biomarkers in Heart Failure Diagnosis and Treatment.

Genet Res (Camb) 2022 16;2022:8727566. Epub 2022 May 16.

Department of Cardiology, Changshu No. 2 People's Hospital, 18 Taishan Road, Changshu, Jiangsu 215000, China.

Background: Heart failure (HF) is defined as the inability of the heart's systolic and diastolic function to properly discharge blood flow from the veins to the heart. The goal of our research is to look into the possible mechanism that causes HF.

Methods: The GSE5406 database was used for screening the differentially expressed genes (DEGs). Gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Protein-Protein Interaction (PPI) network were applied to analyze DEGs. Besides, cell counting Kit-8 (CCK-8) was conducted to observe the knockdown effect of hub genes on cell proliferation.

Results: Finally, 377 upregulated and 461 downregulated DEGs came out, enriched in the extracellular matrix organization and gap junction. According to GSEA results, Hoft cd4 positive alpha beta memory t cell bcg vaccine age 18-45 yo id 7 dy top 100 deg ex vivo up, Sobolev t cell pandemrix age 18-64 yo 7 dy dn, and so on were significantly related to gene set GSE5406. 7 hub genes, such as COL1A1, UBB, COL3A1, HSP90AA1, MYC, STAT3 and MAPK1, were selected from PPI networks. CCK-8 indicated silencing of STAT3 promoted the proliferation of H9C2 cells and silencing of UBB inhibited the proliferation of H9C2 cells.

Conclusion: Our analysis reveals that COL1A1, UBB, COL3A1, HSP90AA1, MYC, STAT3, and MAPK1 might promote the progression of HF and become the biomarkers for diagnosis and treatment of HF.
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http://dx.doi.org/10.1155/2022/8727566DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9126668PMC
June 2022

The involvement of nitric oxide and ethylene on the formation of endodermal barriers in response to Cd in hyperaccumulator Sedum alfredii.

Environ Pollut 2022 Aug 27;307:119530. Epub 2022 May 27.

Ministry of Education Key Laboratory of Environmental Remediation and Ecological Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, 310058, China; National Demonstration Center for Experimental Environment and Resources Education, Zhejiang University, Hangzhou, 310058, China; Zhejiang Provincial Key Laboratory of Agricultural Resources and Environment, Hangzhou, 310058, China. Electronic address:

Nitric oxide (NO) and ethylene are both important signaling molecules which participate in numerous plant development processes and environmental stress resistance. Here, we investigate whether and how NO interacts with ethylene during the development of endodermal barriers that have major consequences for the apoplastic uptake of cadmium (Cd) in the hyperaccumulator Sedum alfredii. In response to Cd, an increased NO accumulation, while a decrease in ethylene production was observed in the roots of S. alfredii. Exogenous supplementation of NO donor SNP (sodium nitroprusside) decreased the ethylene production in roots, while NO scavenger cPTIO (2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide) had the opposite effect. The exogenous addition of NO affected the ethylene production through regulating the expression of genes related to ethylene synthesis. However, upon exogenous ethylene addition, roots retained their NO accumulation. The abovementioned results suggest that ethylene is downstream of the NO signaling pathway in S. alfredii. Regardless of Cd, addition of SNP promoted the deposition of endodermal barriers via regulating the genes related to Casparian strips deposition and suberization. Correlation analyses indicate that NO positively modifies the formation of endodermal barriers via the NO-ethylene signaling pathway, Cd-induced NO accumulation interferes with the synthesis of ethylene, leading to a deposition of endodermal barriers in S. alfredii.
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http://dx.doi.org/10.1016/j.envpol.2022.119530DOI Listing
August 2022

A 24-to-30 GHz Ultra-High-Linearity Down-Conversion Mixer for 5G Applications Using a New Linearization Method.

Sensors (Basel) 2022 May 17;22(10). Epub 2022 May 17.

State Key Laboratory of Mobile Network and Mobile Multimedia Technology, ZTE Corporation, Shenzhen 518000, China.

The linearity of active mixers is usually determined by the input transistors, and many works have been proposed to improve it by modified input stages at the cost of a more complex structure or more power consumption. A new linearization method of active mixers is proposed in this paper; the input 1 dB compression point (IP1dB) and output 1 dB compression point (OP1dB) are greatly improved by exploiting the "reverse uplift" phenomenon. Compared with other linearization methods, the proposed one is simpler, more efficient, and sacrifices less conversion gain. Using this method, an ultra-high-linearity double-balanced down-conversion mixer with wide IF bandwidth is designed and fabricated in a 130 nm SiGe BiCMOS process. The proposed mixer includes a Gilbert-cell, a pair of phase-adjusting inductors, and a Marchand-balun-based output network. Under a 1.6 V supply voltage, the measurement results show that the mixer exhibits an excellent IP1dB of +7.2~+10.1 dBm, an average OP1dB of +5.4 dBm, which is the state-of-the-art linearity performance in mixers under a silicon-based process, whether active or passive. Moreover, a wide IF bandwidth of 8 GHz from 3 GHz to 11 GHz was achieved. The circuit consumes 19.8 mW and occupies 0.48 mm, including all pads. The use of the "reverse uplift" allows us to implement high-linearity circuits more efficiently, which is helpful for the design of 5G high-speed communication transceivers.
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http://dx.doi.org/10.3390/s22103802DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9145764PMC
May 2022

Preparation of DNC Solid Dispersion by a Mechanochemical Method with Glycyrrhizic Acid and Polyvinylpyrrolidone to Enhance Bioavailability and Activity.

Polymers (Basel) 2022 May 16;14(10). Epub 2022 May 16.

National Engineering Research Center for Process Development of Active Pharmaceutical Ingredients, Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Zhejiang University of Technology, Hangzhou 310000, China.

To exploit aqueous-soluble formulation and improve the anticoccidial activity of 4,4'-dinitrocarbanilide (DNC, active component of nicarbazin), this paper prepared DNC/GA/PVP K30 solid dispersion (SD) with glycyrrhizic acid (GA) and polyvinylpyrrolidone (PVP) K30 by a mechanical ball milling method without using any organic solvent. Fourier transform infrared spectroscopy, X-ray diffraction, differential scanning calorimetry, and scanning electron microscopy were used for the solid state characterization. High performance liquid chromatography, critical micelle concentration, particle characterization, and transmission electron microscopy were used to evaluate the behavior in aqueous solution. In addition, the oral bioavailability, tissue distribution, and anticoccidial activity of DNC/GA/PVP K30 SD were investigated as well. Compared with free drug, the novel formulation not only improved the solubility and dissolution rate of DNC, but also inhibited the fecal output of oocysts and enhanced the therapeutic effect of coccidiosis. According to the experiment results, the DNC/GA/PVP K30 SD increased 4.64-fold in oral bioavailability and dramatically enhanced the concentration in liver which provided a basis for further research in schistosomiasis. In summary, our findings suggested that DNC/GA/PVP K30 SD may have promising applications in the treatment of coccidiosis.
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http://dx.doi.org/10.3390/polym14102037DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9145361PMC
May 2022

Clinical Experience and Management Strategy of Retroperitoneal Tumor With Venous Tumor Thrombus Involvement.

Front Oncol 2022 10;12:873729. Epub 2022 May 10.

Department of Urology, Peking University Third Hospital, Beijing, China.

Background: This study aims to report the surgical management, complications, and outcomes for patients with retroperitoneal tumor and venous thrombus.

Methods: We retrospectively analyzed 19 cases of retroperitoneal tumor with venous tumor thrombus from August 2015 to March 2021. A new tumor thrombus PUTH-RT grading system was proposed on the basis of the characteristics of the surgical techniques.

Results: Two cases of PUTH-RT-1a, two cases of PUTH-RT-1b, six cases of PUTH-RT-2, six cases of PUTH-RT-3, and three cases of PUTH-RT-4 were included. Surgeries were successfully performed in all 19 patients. Among them, five cases (26.3%) were operated a completely laparoscopic approach and 13 cases (68.4%) an open approach. One case (5.3%) was converted from laparoscopic to open approach. Five cases (26.3%) experienced postoperative complications. All patients were followed for a median of 14 months. Cancer-associated death occurred in three cases. Distant metastases occurred in seven cases.

Conclusions: We propose a new tumor thrombus grading system based on the anatomical characteristics of retroperitoneal tumors with venous tumor thrombus. Retroperitoneal tumor resection and removal of venous tumor thrombi are safe and effective for the treatment of such diseases.
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http://dx.doi.org/10.3389/fonc.2022.873729DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9128060PMC
May 2022

Task specific adsorbent based on porous monolith for efficient capture of synthetic colorants in beverages and preserved fruits prior to chromatographic analysis.

J Chromatogr A 2022 Jul 14;1675:463144. Epub 2022 May 14.

Fujian Provincial Key Laboratory for Coastal Ecology and Environmental Studies; Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems; College of the Environment and Ecology, Xiamen University, Xiamen 361005, China. Electronic address:

Efficient capture is an indispensable step in the highly sensitive quantification of synthetic colorants (SCs) in food industry. In this scenario, a new task specific adsorbent based on porous monolith (TSA) was conveniently fabricated and utilized as the adsorptive medium of multiple monolithic fibers solid phase microextraction (MF-SPME) for entrapment of SCs in beverages and preserved fruit samples. Various techniques were employed to characterize the prepared TSA and evidenced there were abundant functional groups, pores and satisfactory surface area. The introduced TSA/MF-SPME presented good entrapment performance towards SCs including sunset yellow, allura red and para red through multiple interactions. The enrichment factors were in ranges of 88-106 and 71-101 for beverage and preserved fruit, respectively. Under the optimized fabrication conditions of TSA and extraction parameters, sensitive and reliable method for the quantification of studied SCs in beverage and preserved fruit samples was developed by the combination of TSA/MF-SPME with HPLC/DAD. Limits of detection were 0.015-0.018 µg/L for beverage and 0.057-0.069 µg/kg for preserved fruit samples. The established approach also possessed some features such as wide linear ranges, good precision, low consumptions of sample and organic solvent. The applicability of suggestion method was verified by the quantification of investigated analytes in various beverage and preserved fruit samples, and good recoveries with different fortified concentrations were achieved.
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http://dx.doi.org/10.1016/j.chroma.2022.463144DOI Listing
July 2022

Inhibition of D-2HG leads to upregulation of a proinflammatory gene signature in a novel HLA-A2/HLA-DR1 transgenic mouse model of IDH1R132H-expressing glioma.

J Immunother Cancer 2022 05;10(5)

Neurological Surgery, University of California San Francisco, San Francisco, California, USA

Background: Long-term prognosis of WHO grade II, isocitrate dehydrogenase (IDH)-mutated low-grade glioma (LGG) is poor due to high risks of recurrence and malignant transformation into high-grade glioma. Immunotherapy strategies are attractive given the relatively intact immune system of patients with LGG and the slow tumor growth rate. However, accumulation of the oncometabolite D-2-hydroxyglutarate (D-2HG) in IDH-mutated gliomas leads to suppression of inflammatory pathways in the tumor microenvironment, thereby contributing to the 'cold' tumor phenotype. Inhibiting D-2HG production presents an opportunity to generate a robust antitumor response following tumor antigen vaccination and immune checkpoint blockade.

Methods: An IDH1 glioma model was created in syngeneic -transgenic mice, allowing us to evaluate the vaccination with the human leukocyte antigens (HLA)-DR1-restricted, IDH1 mutation-derived neoepitope. The effects of an orally available inhibitor of mutant IDH1 and IDH2, AG-881, were evaluated as monotherapy and in combination with the IDH1 peptide vaccination or anti-PD-1 immune checkpoint blockade.

Results: The -syngeneic IDH1 cell line expressed the IDH1 mutant protein and formed D-2HG producing orthotopic gliomas in vivo. Treatment of tumor-bearing mice with AG-881 resulted in a reduction of D-2HG levels in IDH1 glioma cells (10 fold) and tumor-associated myeloid cells, which demonstrated high levels of intracellular D-2HG in the IDH1 gliomas. AG-881 monotherapy suppressed the progression of IDH1 gliomas in a CD4 and CD8 cell-dependent manner, enhanced proinflammatory IFNγ-related gene expression, and increased the number of CD4 tumor-infiltrating T-cells. Prophylactic vaccination with the HLA-DR1-restricted IDH1 peptide or tumor-associated HLA-A2-restricted peptides did not enhance survival of tumor-bearing animals; however, vaccination with both HLA-A2-IDH1 and DR1-IDH1 peptides in combination with the IDH inhibitor significantly prolonged survival. Finally, tumor-bearing mice treated with both AG-881 and a PD-1 blocking antibody demonstrated improved survival when compared with either treatment alone.

Conclusion: The development of effective IDH1-targeting vaccine may be enhanced by integration with HLA class I-restricted cytotoxic T cell epitopes and AG-881. Our -syngeneic IDH1 glioma model should allow us to evaluate key translational questions related to the development of novel strategies for patients with IDH-mutant glioma.
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http://dx.doi.org/10.1136/jitc-2022-004644DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9174833PMC
May 2022

An Integrated Immune-Related Bioinformatics Analysis in Glioma: Prognostic Signature's Identification and Multi-Omics Mechanisms' Exploration.

Front Genet 2022 3;13:889629. Epub 2022 May 3.

Department of Emergency Medicine, Shangrao Hospital Affiliated to Nanchang University, Shangrao People's Hospital, Shangrao, China.

As the traditional treatment for glioma, the most common central nervous system malignancy with poor prognosis, the efficacy of high-intensity surgery combined with radiotherapy and chemotherapy is not satisfactory. The development of individualized scientific treatment strategy urgently requires the guidance of signature with clinical predictive value. In this study, five prognosis-related differentially expressed immune-related genes (PR-DE-IRGs) (CCNA2, HMGB2, CASP3, APOBEC3C, and BMP2) highly associated with glioma were identified for a prognostic model through weighted gene co-expression network analysis, univariate Cox and lasso regression. Kaplan-Meier survival curves, receiver operating characteristic curves and other methods have shown that the model has good performance in predicting the glioma patients' prognosis. Further combined nomogram provided better predictive performance. The signature's guiding value in clinical treatment has also been verified by multiple analysis results. We also constructed a comprehensive competing endogenous RNA (ceRNA) regulatory network based on the protective factor BMP2 to further explore its potential role in glioma progression. Numerous immune-related biological functions and pathways were enriched in a high-risk population. Further multi-omics integrative analysis revealed a strong correlation between tumor immunosuppressive environment/IDH1 mutation and signature, suggesting that their cooperation plays an important role in glioma progression.
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http://dx.doi.org/10.3389/fgene.2022.889629DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9114310PMC
May 2022

The effects of Jiawei Duhuo Jisheng mixture on Wnt/β-catenin signaling pathway in the synovium inflamed by knee osteoarthritis: An in vitro and in vivo experiment.

J Ethnopharmacol 2022 Aug 10;294:115363. Epub 2022 May 10.

Hunan University of TCM, Changsha, Hunan, 410208, China; Department of Orthopedic Surgery, Affiliated Hospital of Hunan Academy of Chinese Medical Science, Changsha, Hunan, 410006, China. Electronic address:

Ethnopharmacological Relevance: Knee osteoarthritis (KOA) is one of the common age-degenerative diseases. Recent studies have demonstrated that the pathogenesis of KOA is closely related to synovial lesions. Jiawei Duhuo Jisheng mixture (JDJM) has shown great potential in the treatment of KOA. However, the effect and mechanism of JDJM on synovial lesions of KOA remain unclear.

Aim Of The Study: The regulatory effect of JDJM on the Wnt/β-catenin signaling pathway in KOA inflamed synovium was studied via in vitro and in vivo experiments, respectively.

Materials And Methods: For the in vitro experiment, fibroblasts were isolated from the rabbit synovium with KOA. The fibroblasts were grouped as follows: the vehicle group was given 0.5% FBS; the inhibitor group was treated with 0.5% volume fraction of XAV939; the normal serum groups and JDJM serum groups were treated with 5%, 10%, and 20% volume fractions of normal serum and JDJM-containing serum. The expression levels of Wnt3a, β-catenin, Cyclin D1, metalloproteinase-7(MMP-7) and cyclooxygenase-2(COX-2) were detected by different assays 48 and 72 h after the intervention. For the in vivo experiment, the rabbit KOA model was prepared using the improved Hulth modeling method, whereby all rabbits were randomly divided into normal control, model control, positive control, and traditional Chinese medicine (TCM) groups. The expression levels of Wnt3a, β-catenin, Cyclin D1, MMP-7 and COX-2 were detected by different assays in the 2, 4, and 8 weeks of treatment.

Results: In the two test results of in vitro experiments, the normal serum group was compared with the JDJM-containing serum group with the same volume fraction, demonstrating that mRNA transcription and protein expression levels of Wnt3a, β-catenin, Cyclin D1, MMP-7, and COX-2 in the latter decreased (P < 0.05), with more pronounced effects observed in the group treated with 20% volume fraction of JDJM serum. Compared with the inhibitor group, there was no significant difference (P > 0.05) in the mRNA transcription and protein expression levels, i.e., Wnt3a, β-catenin, Cyclin D1, and MMP-7 were observed in the JDJM serum groups, except for a significant decrease (P < 0.05) in the level of mRNA transcription and protein expression of COX-2. Based on the in vivo experiment, compared to the model control group, articular cartilage, synovial hyperplasia, and the inflammatory reaction of the TCM group at different treatment times were significantly improved. The mRNA transcription level of Wnt3a, β-catenin, Cyclin D1, MMP-7 and COX-2 detected by RT-qPCR and the protein expression level of Wnt3a, β-catenin, Cyclin D1, MMP-7 and COX-2 detected by Western blot were significantly reduced (P < 0.05), and the effect was more evident at the eighth week.

Conclusion: JDJM can regulate the synovial Wnt/β-catenin signaling pathway in KOA models, reduce the mRNA transcription and protein expression levels of Wnt3a, β-catenin, Cyclin D1, MMP-7, and COX-2 in the synovium, thus inhibiting synovial inflammation and protecting articular cartilage, which could be the key mechanism of action in treating this disease.
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http://dx.doi.org/10.1016/j.jep.2022.115363DOI Listing
August 2022

Hypoxia-Responsive Stereocomplex Polymeric Micelles with Improved Drug Loading Inhibit Breast Cancer Metastasis in an Orthotopic Murine Model.

ACS Appl Mater Interfaces 2022 May 27;14(18):20551-20565. Epub 2022 Apr 27.

Institute of Special Environmental Medicine and Co-innovation Center of Neuroregeneration, Nantong University, Nantong 226019, People's Republic of China.

Tumor metastasis is a leading cause of breast cancer-related death. Taxane-loaded polymeric formulations, such as Genexol PM and Nanoxel M using poly(ethylene glycol)-poly(d,l-lactide) (PEG-PLA) micelles as drug carriers, have been approved for the treatment of metastatic breast cancer. Unfortunately, the physical instability of PEG-PLA micelles, leading to poor drug loading, premature drug leakage, and consequently limited drug delivery to tumors, largely hinders their therapeutic outcome. Inspired by the enantiomeric nature of PLA, this work developed stereocomplex PEG-PLA micelles through stereoselective interactions of enantiomeric PLA, which are further incorporated with a hypoxia-responsive moiety used as a hypoxia-cleavable linker of PEG and PLA, to maximize therapeutic outcomes. The results showed that the obtained micelles had high structural stability, showing improved drug loading for effective drug delivery to tumors as well as other tissues. Especially, they were capable of sensitively responding to the hypoxic tumor environment for drug release, reversing hypoxia-induced drug resistance and hypoxia-promoted cell migration for enhanced bioavailability under hypoxia. results further showed that the micelles, especially at a high dose, inhibited the growth of the primary tumor and improved tumor pathological conditions, consequently remarkably inhibiting its metastasis to the lungs and liver, while not causing any systemic toxicity. Hypoxia-responsive stereocomplex micelles thus emerge as a reliable drug delivery system to treat breast cancer metastasis.
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http://dx.doi.org/10.1021/acsami.1c23737DOI Listing
May 2022

Clinicopathological Features of Papillary Renal Cell Carcinoma With Venous Tumor Thrombus: Case Series from a Large Chinese Center.

Clin Med Insights Oncol 2022 17;16:11795549221092217. Epub 2022 Apr 17.

Department of Urology, Peking University Third Hospital, Beijing, P.R. China.

Background: Few studies have reported the influence of the histological classification of type-2 papillary renal cell carcinoma (PRCC), which may differ from that of clear cell renal carcinoma (ccRCC), on the prognosis of renal cell carcinoma with tumor thrombus. We investigated the clinicopathological features and prognosis of type-2 PRCC associated with venous tumor thrombi (PRCC-TT).

Methods: We retrospectively analyzed 163 patients with renal cell carcinoma with venous tumor thrombus (RCC-TT) admitted to Peking University Third Hospital between June 2016 and June 2020. Twenty-five patients had type-2 PRCC-TT and 138 had ccRCC combined with tumor thrombus; there were 125 males and 38 females. All the included patients underwent radical nephrectomy and thrombectomy under either complete laparoscopic surgery or open surgery. Univariate and multivariate Cox regression analysis were performed to evaluate the prognostic significance of each variable on cancer-specific survival (CSS). Cancer-specific survival was calculated from the date of surgery to death or the last follow-up using the Kaplan-Meier method.

Results: The blood vessels of type-2 PRCC-TT presented on CT images were not as abundant as those of ccRCC-TT. Slight enhancement was observed in the corticomedullary phase. While wash-out symptoms were observed, contrast agent extinction was not obvious in the nephrographic and excretory phases. We compared the macroscopic and microscopic appearances of the 2 cohorts. Compared to the ccRCC-TT cohort, lymph node invasion was more prevalent in the PRCC-TT cohort (88.0% vs. 60.9%,  = .009). Multivariate analysis revealed that sarcomatoid differentiation, distant metastasis, and pathological type were the independent predictors of poor CSS. The Kaplan-Meier analysis showed that the CSS of type-2 PRCC-TT and ccRCC-TT were 23.5 and 38.4 months, respectively, with statistical significance ( = .002).

Conclusion: Type-2 PRCC-TT varies with common ccRCC-TT in imaging manifestation and pathological characteristics. The prognosis of type-2 PRCC-TT patients was worse than that of ccRCC-TT patients.
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http://dx.doi.org/10.1177/11795549221092217DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9019393PMC
April 2022

Preoperative Metabolic Syndrome and HDL-C Level Predict the Prognosis of Patients Following Radical Cystectomy: A Propensity Score Matching Study.

Front Oncol 2022 5;12:833305. Epub 2022 Apr 5.

Department of Urology, Peking University Third Hospital, Beijing, China.

Objective: To investigate the prognostic significance of metabolic syndrome (MetS) and its components in patients with bladder cancer (BCa) treated with radical cystectomy (RC).

Methods: A total of 335 BCa patients who underwent RC between 2004 and 2019 at Peking University Third Hospital (PUTH) were analyzed retrospectively. The Kaplan-Meier method with the log-rank test was performed to assess overall survival (OS) and progression-free survival (PFS). Univariate and multivariate Cox proportional hazard models were conducted to identify the prognostic factors of OS and PFS before and after propensity score matching (PSM).

Results: Enrolled patients were allocated into two groups according to the presence or absence of MetS (n=84 MetS vs n=251 non-MetS), and 82 new matched pairs were identified to balance the baseline characteristics after 1:1 PSM. In the Kaplan-Meier analysis, MetS was associated with better OS (P=0.031) than the group without MetS. In addition, a body mass index (BMI) ≥ 25 was associated with better OS (P=0.011) and PFS (P=0.031), while low high-density lipoprotein cholesterol (HDL-C) was associated with worse OS (P=0.033) and PFS (P=0.010). In all patients, multivariate Cox analysis showed that hemoglobin, pathologic tumor stage and lymph node status were identified as independent prognostic factors for both OS and PFS, while age, MetS and HDL-C were independent prognostic factors only for OS. Reproducible results of multivariate analysis can still be observed in propensity matched patients. The results of further subgroup analysis revealed that the association of MetS with increased OS (P=0.043) and BMI ≥25 with increased OS (P=0.015) and PFS (P=0.029) was observed in non-muscle invasive bladder cancer (NMIBC) patients.

Conclusions: MetS was independently associated with better OS in BCa patients after RC, and HDL-C was the only component of MetS that was independently associated with worse OS. MetS and HDL-C may become reliable prognostic biomarkers of OS in BCa patients after RC to provide individualized prognostication and assist in the formulation of clinical treatment strategies.
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http://dx.doi.org/10.3389/fonc.2022.833305DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9022107PMC
April 2022

Controllable synthesis of hollow spherical nickel chalcogenide (NiS and NiSe) decorated with graphene for efficient supercapacitor electrodes.

RSC Adv 2021 Mar 23;11(20):11786-11792. Epub 2021 Mar 23.

Key Laboratory of Bio-Inspired Smart Interfacial Science and Technology of Ministry of Education, School of Chemistry, Beihang University Beijing 100191 P. R. China

New carbon-loaded nickel chalcogenide electrode materials (NiS/GO and NiSe/rGO) have been synthesized through an easy-to-operate process: NiSe was obtained based on NiS hollow spheres, and was successfully synthesized with l-cysteine assistance under the hydrothermal method at 120 °C. GO of different mass fraction was added together with l-cysteine. The electrochemical performance of NiS/GO and NiSe/rGO has been greatly improved because the formation of a carbon-loaded layer effectively increased the specific surface area and reduced the charge transport resistance. Compared with pure NiS and NiSe, NiS/GO and NiSe/rGO presented much better specific capacitance (1020 F g and 722 F g respectively at a current density of 1 A g) and more superior rate capability (when the current density was raised to 5 A g the specific capacitance remained at 569 F g and 302 F g). This work highlights the advantages of nickel compounds through a very simple experimental method, and contributes to providing a good reference for preparation of superior supercapacitor materials with high performance.
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http://dx.doi.org/10.1039/d0ra10659cDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8696559PMC
March 2021
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