Publications by authors named "Min Lu"

912 Publications

Balanced Charge Transport and Enhanced Performance of Blue Quantum Dot Light-Emitting Diodes via Electron Transport Layer Doping.

Nanotechnology 2021 May 10. Epub 2021 May 10.

Louisiana State University Shreveport, Shreveport, UNITED STATES.

The unbalanced charge transport is always a key influence factor on the device performance of quantum dot light-emitting diodes (QLEDs), particularly for the blue QLEDs due to their large optical band gap. Here, a method of electron transport layers (ETL) doping was developed to regulate the energy levels and the carrier mobility of the ETL, which result in more balanced charge injection, transport and recombination in the blue emitting CdZnS/ZnS core/shell QLEDs. Consequently, an enhanced performance of blue QLEDs were achieved by modulating the charge balance through ETL doping. The maximum external quantum efficiency and luminance have been dramatically increased from 2.2% to 7.3% and from 3786 cd m-2 to 9108 cd m-2, respectively. The results illustrate that charge transport layers doping is a simple and effective strategy to regulate the charge injection barrier and carrier mobility of QLED.
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http://dx.doi.org/10.1088/1361-6528/abff8cDOI Listing
May 2021

Individual-level morphological hippocampal networks in patients with Alzheimer's disease.

Brain Cogn 2021 May 7;151:105748. Epub 2021 May 7.

Department of Radiology, Yantai Yuhuangding Hospital, Affiliated Hospital of Qingdao University, Yantai, Shandong 264000, China. Electronic address:

In patients with Alzheimer's Disease (AD), the hippocampal network has been extensively investigated in previous studies; however, little is known about the morphological network associated with the hippocampus in the AD patients. A total of 68 patients with AD and another 68 gender and age matched healthy subjects were studied. Individual-level morphological hippocampal networks were constructed based on volume and texture features extracted from MRI to study the connections between bilateral hippocampus and 11 other subcortical gray matter structures. The relationship between morphological connections and Mini-Mental State Examination (MMSE) scores was also studied. Connections between bilateral hippocampus and bilateral thalamus, bilateral putamen were significant differences between the AD patients and controls (p < 0.05). There were significantly different in bilateral hippocampal connectivity, and for the left hippocampus, the connection to the right caudate were found to be statistically significant. The morphological connections between left hippocampus and bilateral thalamus (left: R = 0.371, p < 0.001; right: R = 0.411, p < 0.001), bilateral putamen (left: R = 0.383, p < 0.001; right: R = 0.348, p < 0.001), right hippocampus and bilateral thalamus (left: R = 0.370, p < 0.001; right: R = 0.387, p < 0.001), left putamen (R = 0.377, p < 0.001) were significantly positively correlated with the MMSE scores. Similar patterns were observed for left and right hippocampal connectivity and the connections highly associated with MMSE scores were also within the abnormal connections in AD patients.
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http://dx.doi.org/10.1016/j.bandc.2021.105748DOI Listing
May 2021

Comparative ultrastructure of trichomes on various organs of Rosa roxburghii.

Microsc Res Tech 2021 May 2. Epub 2021 May 2.

Agricultural College, Guizhou University, Guiyang, China.

Chestnut rose, R. roxburghii Tratt. (Rosaceae) (RR) is an important crop in China due to its nutritional and medicinal values. RR frequently produces trichomes on the surfaces of a diverse range of organs, however a genetic component exists to the control of trichome development, with some cultivars having significantly fewer trichomes to others. Certain varieties have fruits that are thickly covered with macroscopic trichomes, which is an undesirable trait for fruit processing and consumption. However, smooth-fruit cultivars exist, such as R. roxburghii Tratt. f. esetosa Ku (RRE). Despite their economic importance, the anatomical features of trichomes have not been explored in detail for these two chestnut rose germplasms. Here, we investigate the ultrastructure of trichomes distributed on the stem, sepal, and fruit of RR and RRE using transmission electron microscopy (TEM). The internal structure of stem prickle trichomes in RR and RRE was oval in shape and did not contain nucleoli or other organelles. The cell walls of stem prickles in RR are thick and the intercellular spaces occupied with liquid, whereas the cells wall of stem prickles in RRE are thin and have air-filled intercellular spaces. The cells of sepal acicular trichomes in RR and glandular trichomes (GTs) of sepals in RRE had similar vacuole sizes, cytoplasm content, intercellular spaces, and arrangement of plastids within cells. However, there were osmiophilic granules present in the GTs of RRE. The flagelliform trichomes in the sepals of the two germplasms are composed of oval or rod-shaped cells. Although the flagelliform trichomes in the sepals of the two germplasms had a similar internal structure, and both contained starch grains and plastids with visible thylakoid membranes, the flagelliform trichomes in the sepals of RR had a thinner cell wall and a higher proportion of cytoplasm which was more evenly distributed across the cell. There were granules that stained heavily with osmium tetroxide which occurred infrequently in the flagelliform trichomes of sepals in RRE but were not observed in RR. On the acicular trichomes of fruit in RR, the flagelliform trichomes and the GTs of fruit in RRE shared similar cell morphology, arrangement and vacuole size as well as intercellular space. Both the fruit flagelliform trichomes and GTs in RRE contain granules which stain heavily with osmium tetroxide, and the GTs contain plastids and starch grains. These differences in trichome cell ultrastructure may be related to developmental processes or biological functions of the trichomes. These results also suggest that the two chestnut rose germplasms are good candidates for further study of trichome ontogeny in the genus and subsequent breeding of the smooth organ trait in this species.
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http://dx.doi.org/10.1002/jemt.23765DOI Listing
May 2021

Alendronate-functionalized hypoxia-responsive polymeric micelles for targeted therapy of bone metastatic prostate cancer.

J Control Release 2021 Apr 29;334:303-317. Epub 2021 Apr 29.

Institute of Special Environmental Medicine, Nantong University, Nantong, People's Republic of China. Electronic address:

Bone metastasis is one of the leading causes of cancer-related death and remains incurable in spite of great efforts. Bone-targeted nanoparticle-based drug carriers can overcome the difficulties in delivering therapeutic agents to metastatic bone and endowing them with a stimuli-responsive feature for controllable drug release can further maximize their therapeutic outcome. In light of hypoxic microenvironment of bone metastasis, we herein reported a bone-targeted and hypoxia-responsive polymeric micelle system for effective treatment of bone metastatic prostate cancer. The micelles were self-assembled from a polyethylene glycol and poly-l-lysine based copolymer using alendronate as a bone-targeted moiety and azobenzene as a hypoxia-responsive linker, showing a high affinity to metastatic bone and a high sensitivity in responding to hypoxia in vitro. In vivo studies further showed that after a selective accumulation in metastatic bone, the micelles could respond to hypoxic bone metastasis for rapid drug release to an effective therapeutic dosage. As a result, the micelles could suppress tumor growth in bone and inhibit bone destruction by inhibiting osteoclast activity and promoting osteoblast activity, achieving an enhanced therapeutic outcome with relieved bone pain and prolonged survival time. Bone-targeted and hypoxia-responsive nanocarriers therefore represent a promising advancement for treating bone metastasis. To our best knowledge, it might be the first example of the application of hypoxia-responsive nanocarriers in treating bone metastasis.
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http://dx.doi.org/10.1016/j.jconrel.2021.04.035DOI Listing
April 2021

Neuroprotective Effects of Oxymatrine on PI3K/Akt/mTOR Pathway After Hypoxic-Ischemic Brain Damage in Neonatal Rats.

Front Pharmacol 2021 13;12:642415. Epub 2021 Apr 13.

Department of Pharmacology, Ningxia Medical University, Yinchuan, China.

Oxymatrine (OMT), a quinolizidine alkaloid extracted from traditional Chinese herb Ait, has drawn attention because of its beneficial bioactivities against hypoxic-ischemic brain damage (HIBD). However, the underlying molecular mechanism remains unclear. In this study, we determined the and effects of OMT on seven-day old Sprague-Dawley rats with HIBD and in a rat model of primary hippocampal neuron oxygen glucose deprivation reoxygenation (OGD/R). This study was aimed to evaluate whether OMT exerted neuroprotective effects mediated by the (phosphatidylinositol 3-kinase/protein kinase B/mammalian target of rapamycin) PI3K/Akt/mTOR pathway after HIBD. Experimental results showed that the alkaloid significantly improved the early neurofunctional development, brain water content, abnormal pathological changes, and necrosis of neurons after HIBD. Moreover, OMT enhanced the cell viability and stabilized the mitochondrial permeability transition pore in the primary hippocampal neurons after OGD/R. OMT significantly decreased the autophagosome generation, elevated the expression of PI3K, Akt, and mTOR, and simultaneously reversed the mRNA expression of microtubule-associated protein 1-light chain 3 (LC3), Beclin-1, and sequestosomel (P62) induced by hypoxia and ischemia. However, these protective effects against HIBD could be suppressed when rapamycin, a specific inhibitor of mTOR, was included. Hence, the OMT exerted neuroprotective effects against HIBD by attenuating excessive autophagy by mediating the PI3K/Akt/mTOR pathway.
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http://dx.doi.org/10.3389/fphar.2021.642415DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8077028PMC
April 2021

Juglone regulates gut microbiota and Th17/Treg balance in DSS-induced ulcerative colitis.

Int Immunopharmacol 2021 Apr 26;97:107683. Epub 2021 Apr 26.

Affliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, Jiangsu, PR China. Electronic address:

Juglone, mainly isolates from the green walnut husks of Juglans mandshurica, exhibits anti-cancer and anti-inflammaroty activities. But its protection on ulcerative colitis (UC) has never been explored. In this study, we first evaluated whether juglone ameliorated UC, and investigated its effects on gut microbiota and Th17/Treg balance in DSS-induced UC mice model. The model was established by administrating 2.7% DSS for seven days. Juglone was given daily by gavage for ten days, once a day. The disease activity index (DAI) decrease and pathological characteristics improvement demonstrated that the UC in mice was alleviated by juglone. Juglone treatment significantly inhibited the protein levels of IL-6, TNF-α and IL-1β, improved the protein expression of IL-10. In addition, juglone altered microbial diversity and gut microbiota composition, including the enhancement of the ratio of Firmicutes to Bacteroidota and the abundance of Actinobacteriota, and decrease of the abundance of Verrucomicrobiota. Juglone treatment also inhibited the protein expressions of IL-6, STAT3 and RORγt, meanwhile improved the protein level of FOXP3. Furthermore, juglone inhibited Th17 development and increased Treg generation, beneficial to Th17/Treg balance. Together, we herein provided the first evidence to support that juglone, especially the high dose, possibly protected mice against UC by modulating gut microbiota and restoring Th17/Treg homeostasis.
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http://dx.doi.org/10.1016/j.intimp.2021.107683DOI Listing
April 2021

Determination of lipid-water partition coefficient of neutral and ionic drugs by liposome electrokinetic chromatography.

Electrophoresis 2021 Apr 28. Epub 2021 Apr 28.

School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, P. R. China.

Profiling of lipid-water partition coefficients (K ) of drugs is an essential issue during the early stage of drug development. In this study, two liposomes, including 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) + cholesterol (Chol) (DSPC/Chol liposomes) and soybean lecithin (SPC) + Chol (SPC/Chol liposomes), were prepared for the liposome electrokinetic chromatography (LEKC) analysis, and the logarithm of lipid-water partition coefficients (log K ) of neutral and ionic drugs were determined based on an iterative method. The log K values determined by the SPC/Chol or DSPC/Chol liposomes LEKC were linearly fitted, which showed a good fitting coefficient (R = 0.89). Furthermore, the linear relationship between the data obtained from LEKC system and octanol-water system, immobilized artificial membrane, Caco-2 cell model, and software prediction was analyzed, respectively. Results illustrated that DSPC/Chol liposomes or SPC/Chol liposomes had a good linear relationship with Caco-2 cell model, and R was 0.81 and 0.72, respectively. Moreover, the linear free energy relationship analysis suggested that the solute volume, hydrogen bond basicity, and J were the main descriptors that drove the partition process of solutes in the SPC/Chol or DSPC/Chol LEKC system. In addition, the normalized properties of the SPC/Chol and DSPC/Chol LEKC systems through linear free energy relationship analysis were very close. In short, DSPC/Chol liposomes are more suitable for simulating cell membranes than SPC/Chol liposomes, and the developed LEKC is an effective partitioning model for measuring the log K of drugs.
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http://dx.doi.org/10.1002/elps.202000382DOI Listing
April 2021

Sec62 promotes stemness and chemoresistance of human colorectal cancer through activating Wnt/β-catenin pathway.

J Exp Clin Cancer Res 2021 Apr 15;40(1):132. Epub 2021 Apr 15.

Hepatopancreatobiliary Surgery Department I, Key laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Peking University Cancer Hospital & Institute, Beijing, 100142, China.

Background: Cancer stem cell (CSC)-related chemoresistance leads to poor outcome of the patients with colorectal cancer (CRC). In this study, we identified the chemoresistance-relevant molecules and decipher the involved mechanisms to provide potential therapeutic target for CRC. We focused on Sec62, a novel target with significantly increased expression in chemoresistant CRC tissues, and further investigated its role in the progression of CRC.

Methods: Through analyzing the differentially-expressed genes between chemoresistant and chemosensitive CRCs, we selected Sec62 as a novel chemoresistance-related target in CRC. The expression and clinical significance of Sec62 were determined by immunoblotting and immunohistochemistry in tissues and cell lines of CRC. The roles of Sec62 in drug resistance, stemness and tumorigenesis were evaluated in vitro and in vivo using functional experiments. GST pull-down, western blot, coimmunoprecipitation and Me-RIP assays were performed to further explore the downstream molecular mechanisms.

Results: Sec62 upregulation was associated with the chemoresistance of CRC and poor outcome of CRC patients. Depletion of Sec62 sensitized CRC cells to chemotherapeutic drugs. Sec62 promoted the stemness of CRC cells through activating Wnt/β-catenin signaling. Mechanistically, Sec62 bound to β-catenin and inhibited the degradation of β-catenin. Sec62 competitively disrupted the interaction between β-catenin and APC to inhibit the β-catenin destruction complex assembly. Moreover, Sec62 expression was upregulated by the mA-mediated stabilization of Sec62 mRNA.

Conclusions: Sec62 upregulated by the METTL3-mediated mA modification promotes the stemness and chemoresistance of CRC by binding to β-catenin and enhancing Wnt signalling. Thus, mA modification-Sec62-β-catenin molecular axis might act as therapeutic targets in improving treatment of CRC.
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http://dx.doi.org/10.1186/s13046-021-01934-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8051072PMC
April 2021

Structure-Based Design of Selective LONP1 Inhibitors for Probing Biology.

J Med Chem 2021 Apr 6;64(8):4857-4869. Epub 2021 Apr 6.

Genomics Institute of the Novartis Research Foundation, 10675 John J. Hopkins Dr., San Diego, California 92121, United States.

LONP1 is an AAA+ protease that maintains mitochondrial homeostasis by removing damaged or misfolded proteins. Elevated activity and expression of LONP1 promotes cancer cell proliferation and resistance to apoptosis-inducing reagents. Despite the importance of LONP1 in human biology and disease, very few LONP1 inhibitors have been described in the literature. Herein, we report the development of selective boronic acid-based LONP1 inhibitors using structure-based drug design as well as the first structures of human LONP1 bound to various inhibitors. Our efforts led to several nanomolar LONP1 inhibitors with little to no activity against the 20S proteasome that serve as tool compounds to investigate LONP1 biology.
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http://dx.doi.org/10.1021/acs.jmedchem.0c02152DOI Listing
April 2021

Preparation of phytic acid modified α-Glucosidase/Cu(PO)·3HO hybrid nanoflower and its application.

Enzyme Microb Technol 2021 May 10;146:109776. Epub 2021 Mar 10.

School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, 401331, China. Electronic address:

A simple, convenient and efficient enzyme immobilization method through phytic acid (PA) modified α-Glucosidase (α-Glu)/Cu(PO)·3HO hybrid nanoflower was developed. The structural properties of the materials were studied by several characterization techniques. Subsequently, the enzymatic reaction conditions such as the pH value and temperature were optimized, and the enzyme kinetics and inhibition parameters were determined. The PA modified α-Glu/Cu(PO)·3HO hybrid nanoflower had better enzymatic activity under a wide pH range and high temperature than the free one. After seven successive cycles, the PA modified α-Glu/Cu(PO)·3HO hybrid nanoflower could still maintain approximately 63.0 % of its initial immobilized enzyme activity. The Michaelis-Menten constant (K) and the half-maximal inhibitory concentration (IC) of acarbose were determined as 0.77 mM and 15.01 μM, respectively. In addition, the material was applied to evaluate the inhibitory activity of ten phenolic compounds on α-Glu, and epicatechin gallate, gallocatechin gallate, epigallocatechin gallate and rosmarinic acid showed good inhibitory activity with % of inhibition of (53.42 ± 2.39)%, (37.28 ± 1.32)%, (37.08 ± 0.63)% and (35.53 ± 0.23)%, respectively. These results indicate that the PA modified hybrid nanoflower is an efficient method of α-Glu immobilization.
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http://dx.doi.org/10.1016/j.enzmictec.2021.109776DOI Listing
May 2021

Solubility, Permeability, Anti-Inflammatory Action and In Vivo Pharmacokinetic Properties of Several Mechanochemically Obtained Pharmaceutical Solid Dispersions of Nimesulide.

Molecules 2021 Mar 10;26(6). Epub 2021 Mar 10.

National Engineering Research Center for Process Development of Active Pharmaceutical Ingredients, Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Zhejiang University of Technology, Hangzhou 310014, China.

Nimesulide (NIM, -(4-nitro-2-phenoxyphenyl)methanesulfonamide) is a relatively new nonsteroidal anti-inflammatory analgesic drug. It is practically insoluble in water (<0.02 mg/mL). This very poor aqueous solubility of the drug may lead to low bioavailability. The objective of the present study was to investigate the possibility of improving the solubility and the bioavailability of NIM via complexation with polysaccharide arabinogalactan (AG), disodium salt of glycyrrhizic acid (NaGA), hydroxypropyl-β-cyclodextrin (HP-β-CD) and MgCO. Solid dispersions (SD) have been prepared using a mechanochemical technique. The physical properties of nimesulide SD in solid state were characterized by differential scanning calorimetry and X-ray diffraction studies. The characteristics of the water solutions which form from the obtained solid dispersions were analyzed by reverse phase and gel permeation HPLC. It was shown that solubility increases for all complexes under investigation. These phenomena are obliged by complexation with auxiliary substances, which was shown by H-NMR relaxation methods. The parallel artificial membrane permeability assay (PAMPA) was used for predicting passive intestinal absorption. Results showed that mechanochemically obtained complexes with polysaccharide AG, NaGA, and HP-β-CD enhanced permeation of NIM across an artificial membrane compared to that of the pure NIM. The complexes were examined for anti-inflammatory activity on a model of histamine edema. The substances were administered to CD-1 mice. As a result, it was found that all investigated complexes dose-dependently reduce the degree of inflammation. The best results were obtained for the complexes of NIM with NaGA and HP-β-CD. In noted case the inflammation can be diminished up to 2-fold at equal doses of NIM.
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http://dx.doi.org/10.3390/molecules26061513DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7998760PMC
March 2021

Presacral Tumor: Insights From a Decade's Experience of This Rare and Diverse Disease.

Authors:
Zeyu Li Min Lu

Front Oncol 2021 16;11:639028. Epub 2021 Mar 16.

Department of Colorectal Surgery, First Affiliated Hospital of China Medical University, Shenyang, China.

Background: Presacral tumors are a group of rare and heterogeneous tumors that arise from the potential presacral space between the rectum and sacrum. The low occurrence and diverse origins make the diagnosis and treatment of these tumors a challenge. The aim of the study was to retrospectively review patient demographics and to identify advantages and disadvantages in the diagnosis and treatment of these tumors.

Methods: Retrospectively collected and reviewed data from patients who received treatment of presacral tumors at the First Affiliated Hospital of China Medical University between August 2009 and June 2019.

Results: The data from forty-four patients (33 females) with a median age of 50 years who were diagnosed with a presacral/retrorectal tumor were analyzed. The majority of tumors were congenital (61.4%) and benign tumors are more common (59.1%). The median age of patients with benign tumor was significantly higher than that of malignant tumor. The most common symptoms were sacrococcygeal/perianal pain (56.8%) and mass (36.4%), and 8 out of 9 patients having lower limb symptoms diagnosed with malignant tumor. The tumor detection rate of digital rectal examination was 75% and more than 90% of all patients underwent one or more radiology imaging exams for tumor diagnosis. Every patient had a biopsy result. The most common type of tumor was presacral cyst (40.9%) with overall tumor median size of 5.6 cm. Thirty-one (70.5%) patients underwent surgery, most often the posterior route (83.9%). Posterior route surgery had significantly shorter operation time and tumors operated posterior route were significantly smaller. The survival rate after surgery was 100%. The median course of disease was 6 months and median follow-up was 25 months.

Conclusions: Presacral tumors have low occurrence and are more frequently observed in females in their 30s and 50s indicating a possible link between tumor occurrence and hormonal changes. Patients with lower limb symptoms were more likely to have a malignant presacral tumor. Posterior route was the most commonly utilized surgical approach. Supplementary iodine tincture treatment of cysts ruptured in operation could potentially be helpful in reducing the chance of recurrence.
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http://dx.doi.org/10.3389/fonc.2021.639028DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8008122PMC
March 2021

Transcriptome and lipidome profile of human mesenchymal stem cells with reduced senescence and increased trilineage differentiation ability upon drug treatment.

Aging (Albany NY) 2021 Mar 26;13(7):9991-10014. Epub 2021 Mar 26.

School of Life Science and Technology, Harbin Institute of Technology, Harbin 150080, Heilongjiang, China.

Human Mesenchymal stem cells (hMSCs) are multi-potential cells which are widely used in cell therapy. However, the frequently emerged senescence and decrease of differentiation capabilities limited the broad applications of MSC. Several strategies such as small molecules treatment have been widely studied and used to improve the stem characteristics bypassing the senescence but the exact mechanisms for them to reduce senescence have not been fully studied. In this study, hMSCs were treated by rapamycin, oltipraz, metformin, and vitamin C for the indicated time and these cells were subjected to senescence evaluation and trilineage differentiation. Furthermore, transcriptomics and lipidomics datasets of hMSCs after drug treatment were analyzed to interpret biological pathways responsible for their anti-senescence effects. Although four drugs exhibited significant activities in promoting MSC osteogenic differentiation, metformin is the optimal drug to promote trilineage differentiation. GO terms illustrated that the anti-aging effects of drugs were mainly associated with cellular senescence, mitotic and meiosis process. Biosynthesis of phosphatidylcholines (PC) and phosphatidylethanolamine (PE) were inhibited whereas production of phosphatidylinositols (PIs) and saturated fatty acids (SFA)/ mono-unsaturated fatty acids (MUFA) conversion was activated. Medium free fatty acids (FFA) was increased in hMSCs with different anti-aging phenotypes. Therefore, we established a comprehensive method in assessing drug intervention based on the results of transcriptomics and lipidomics. The method can be used to study different biological phenotypes upon drug intervention in MSC which will extend the clinical application of hMSCs.
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http://dx.doi.org/10.18632/aging.202759DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8064146PMC
March 2021

Survival Disparities in Multiple Myeloma by Health Insurance Status among US Non-Elderly Adults: A SEER-Based Comparative Analysis.

Acta Haematol 2021 Mar 30:1-9. Epub 2021 Mar 30.

Institute for Urban and Population Development, Shanghai Academy of Social Sciences, Shanghai, China.

Background/aim: The impacts of health insurance status on survival outcomes in multiple myeloma (MM) have not been addressed in depth. The present study was conducted to identify definite relationships of cancer-specific survival (CSS) and overall survival (OS) with health insurance status in MM patients.

Methods: MM patients aged 18-64 years and with complete insurance records between January 1, 2007, and December 31, 2016, were identified from 18 Surveillance, Epidemiology, and End Results (SEER) Database registries. Health insurance condition was categorized as uninsured, any Medicaid, insured, and insured (no specifics). Relationships of health insurance condition with OS/CSS were identified through Kaplan-Meier, and uni-/multivariate Cox regressions using the hazard ratio and 95% confidence interval. Potential baseline confounding was adjusted using multiple propensity score (mPS).

Results: Totally 17,981 patients were included, including 68.3% with private insurance and only 4.9% with uninsurance. Log-rank test uncovered significant difference between health insurance status and OS/CSS among MM patients. Patients with non-insurance or Medicaid coverage in comparison with private insurance tended to present poorer OS/CSS both in multivariate Cox regression and in mPS-adjusted model (non-insurance vs. private insurance [OS/CSS]: 1.33 [1.20-1.48]/1.13 [1.00-1.28] and 1.45 [1.25-1.69]/1.18 [1.04-1.33], respectively; Medicaid coverage vs. private insurance [OS/CSS]: 1.67 [1.56-1.78]/1.25 [1.16-1.36] and 1.76 [1.62-1.90]/1.23 [1.13-1.35], respectively).

Conclusions: Our observational study of exposure-outcome associations suggests that insufficient or no insurance is moderately linked with OS among MM patients aged 18-64 years. Wide insurance coverage and health-care availability may strengthen some disparate outcomes. In the future, prospective cohort research is needed to further clarify concrete risks with insurance type, owing to the lack of definite division of insurance data in SEER.
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http://dx.doi.org/10.1159/000514671DOI Listing
March 2021

Silencing of OIP5-AS1 Protects Endothelial Cells From ox-LDL-Triggered Injury by Regulating KLF5 Expression via Sponging miR-135a-5p.

Front Cardiovasc Med 2021 12;8:596506. Epub 2021 Mar 12.

Department of Cardiovascular Comprehensive Ward II, Henan Provincial People's Hospital, Zhengzhou, China.

Long non-coding RNAs (lncRNAs) have been implicated in the pathogenesis of atherosclerosis. LncRNA OIP5 antisense RNA 1 (OIP5-AS1) has been found to be associated with the development of atherosclerosis. In this study, we further investigated the molecular basis of OIP5-AS1 in atherosclerosis pathogenesis. Oxidative low-density lipoprotein (ox-LDL) was used to treat human umbilical vein endothelial cells (HUVECs). The levels of OIP5-AS1, miR-135a-5p, and Krüppel-like factor 5 (KLF5) were detected by quantitative real-time polymerase chain reaction (qRT-PCR) or western blot. Cell viability, migration, and apoptosis were evaluated using the Cell Counting Kit-8 (CCK-8), Transwell, and flow cytometry, respectively. The levels of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and malondialdehyde (MDA) were determined with enzyme-linked immunosorbent assay (ELISA). Targeted interactions among OIP5-AS1, miR-135a-5p, and KLF5 were confirmed by dual-luciferase reporter and RNA immunoprecipitation (RIP) assays. Animal studies were performed to assess the role of OIP5-AS1 in atherosclerosis progression . Our data showed the significant upregulation of OIP5-AS1 in atherosclerosis serum and ox-LDL-stimulated HUVECs. The silencing of OIP5-AS1 protected against ox-LDL-triggered cytotoxicity in HUVECs and diminished lipids secretion in ApoE mice. Moreover, OIP5-AS1 functioned as a molecular sponge of miR-135a-5p, and miR-135a-5p was a functional mediator of OIP5-AS1 in regulating ox-LDL-induced HUVEC injury. KLF5 was a direct target of miR-135a-5p, and the increased expression of miR-135a-5p alleviated ox-LDL-induced cytotoxicity by downregulating KLF5. Furthermore, OIP5-AS1 influenced KLF5 expression through sponging miR-135a-5p. The current work identified that the silencing of OIP5-AS1 protected against ox-LDL-triggered cytotoxicity in HUVECs at least in part by influencing KLF5 expression via acting as a miR-135a-5p sponge.
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http://dx.doi.org/10.3389/fcvm.2021.596506DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7994260PMC
March 2021

Recent Development of Oxygen Evolution Electrocatalysts in Acidic Environment.

Adv Mater 2021 Mar 25:e2006328. Epub 2021 Mar 25.

State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, 730000, China.

The proton exchange membrane (PEM) water electrolysis is one of the most promising hydrogen production techniques. The oxygen evolution reaction (OER) occurring at the anode dominates the overall efficiency. Developing active and robust electrocatalysts for OER in acid is a longstanding challenge for PEM water electrolyzers. Most catalysts show unsatisfied stability under strong acidic and oxidative conditions. Such a stability challenge also leads to difficulties for a better understanding of mechanisms. This review aims to provide the current progress on understanding of OER mechanisms in acid, analyze the promising strategies to enhance both activity and stability, and summarize the state-of-the-art catalysts for OER in acid. First, the prevailing OER mechanisms are reviewed to establish the physicochemical structure-activity relationships for guiding the design of highly efficient OER electrocatalysts in acid with stable performance. The reported approaches to improve the activity, from macroview to microview, are then discussed. To analyze the problem of instability, the key factors affecting catalyst stability are summarized and the surface reconstruction is discussed. Various noble-metal-based OER catalysts and the current progress of non-noble-metal-based catalysts are reviewed. Finally, the challenges and perspectives for the development of active and robust OER catalysts in acid are discussed.
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http://dx.doi.org/10.1002/adma.202006328DOI Listing
March 2021

Bacteria-specific pro-photosensitizer kills multidrug-resistant Staphylococcus aureus and Pseudomonas aeruginosa.

Commun Biol 2021 Mar 25;4(1):408. Epub 2021 Mar 25.

Wellman Center for Photomedicine, Massachusetts General Hospital, Department of Dermatology, Harvard Medical School, Boston, MA, USA.

The emergence of multidrug-resistant bacteria has become a real threat and we are fast running out of treatment options. A combinatory strategy is explored here to eradicate multidrug-resistant Staphlococcus aureus and Pseudomonas aeruginosa including planktonic cells, established biofilms, and persisters as high as 7.5 log bacteria in less than 30 min. Blue-laser and thymol together rapidly sterilized acute infected or biofilm-associated wounds and successfully prevented systematic dissemination in mice. Mechanistically, blue-laser and thymol instigated oxidative bursts exclusively in bacteria owing to abundant proporphyrin-like compounds produced in bacteria over mammalian cells, which transformed harmless thymol into blue-laser sensitizers, thymoquinone and thymohydroquinone. Photo-excitations of thymoquinone and thymohydroquinone augmented reactive oxygen species production and initiated a torrent of cytotoxic events in bacteria while completely sparing the host tissue. The investigation unravels a previously unappreciated property of thymol as a pro-photosensitizer analogous to a prodrug that is activated only in bacteria.
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http://dx.doi.org/10.1038/s42003-021-01956-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7994569PMC
March 2021

Heterologous arenavirus vector prime-boost overrules self-tolerance for efficient tumor-specific CD8 T cell attack.

Cell Rep Med 2021 Mar 3;2(3):100209. Epub 2021 Mar 3.

University of Basel, Department of Biomedicine, Basel, Switzerland.

Therapeutic vaccination regimens inducing clinically effective tumor-specific CD8 T lymphocyte (CTL) responses are an unmet medical need. We engineer two distantly related arenaviruses, Pichinde virus and lymphocytic choriomeningitis virus, for therapeutic cancer vaccination. In mice, life-replicating vector formats of these two viruses delivering a self-antigen in a heterologous prime-boost regimen induce tumor-specific CTL responses up to 50% of the circulating CD8 T cell pool. This CTL attack eliminates established solid tumors in a significant proportion of animals, accompanied by protection against tumor rechallenge. The magnitude of CTL responses is alarmin driven and requires combining two genealogically distantly related arenaviruses. Vector-neutralizing antibodies do not inhibit booster immunizations by the same vector or by closely related vectors. Rather, CTL immunodominance hierarchies favor vector backbone-targeted responses at the expense of self-reactive CTLs. These findings establish an arenavirus-based immunotherapy regimen that allows reshuffling of immunodominance hierarchies and breaking self-directed tolerance for efficient tumor control.
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http://dx.doi.org/10.1016/j.xcrm.2021.100209DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7974551PMC
March 2021

Isolation, Identification, and Analysis of Potential Functions of Culturable Bacteria Associated with an Invasive Gall Wasp, Leptocybe invasa.

Microb Ecol 2021 Mar 23. Epub 2021 Mar 23.

State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, China.

Symbioses between invasive insects and bacteria are one of the key drivers of insect invasion success. Gall-inducing insects stimulate host plants to produce galls, which affects the normal growth of plants. Leptocybe invasa Fisher et La Salle, an invasive gall-inducing wasp, mainly damages Eucalyptus plantations in Southern China, but little is known about its associated bacteria. The aim of this study was to assess the diversity of bacterial communities at different developmental stages of L. invasa and to identify possible ecological functions of the associated bacteria. Bacteria associated with L. invasa were isolated using culture-dependent methods and their taxonomic statuses were determined by sequencing the 16S rRNA gene. A total of 88 species belonging to four phyla, 27 families, and 44 genera were identified by phylogenetic analysis. The four phyla were Proteobacteria, Actinobacteria, Firmicutes, and Bacteroidetes, mainly from the genera Pantoea, Enterobacter, Pseudomonas, Bacillus, Acinetobacter, Curtobacterium, Sphingobium, Klebsiella, and Rhizobium. Among them, 72 species were isolated in the insect gall stage and 46 species were isolated from the adult stage. The most abundant bacterial species were γ-Proteobacteria. We found significant differences in total bacterial counts and community compositions at different developmental stages, and identified possible ecological roles of L. invasa-associated bacteria. This study is the first to systematically investigate the associated bacteria of L. invasa using culture-dependent methods, and provides a reference for other gall-inducing insects and associated bacteria.
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http://dx.doi.org/10.1007/s00248-021-01715-wDOI Listing
March 2021

Genome-Wide Analysis of Genes and Their Response to Abiotic Stress in Rose ().

Front Genet 2021 4;12:538733. Epub 2021 Mar 4.

College of Agriculture, Guizhou University, Guiyang, China.

Dof (DNA binding with one finger) proteins play important roles in plant development and defense regulatory networks. In the present study, we report a genome-wide analysis of rose genes (), including phylogenetic inferences, gene structures, chromosomal locations, gene duplications, and expression diversity. A total of 24 full-length genes were identified in , which were assigned to nine distinct subgroups. These genes were unevenly distributed on rose chromosomes. The genome-scale analysis of synteny indicated that segmental duplication events may have played a major role in the evolution of the gene family. Analysis of -acting elements revealed putative functions of in rose during development as well as under numerous biotic and abiotic stress conditions. Moreover, the expression profiles derived from qRT-PCR experiments demonstrated distinct expression patterns in various tissues, and gene expression divergence existed among the duplicated genes, suggesting a fundamentally functional divergence of the duplicated paralogs in rose. The gene expression analysis of s under drought and salt stress conditions was also performed. The present study offered novel insights into the evolution of s and can aid in the further functional characterization of its candidate genes.
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http://dx.doi.org/10.3389/fgene.2021.538733DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7969895PMC
March 2021

Physiological and metabolomics responses of two wheat (Triticum aestivum L.) genotypes differing in grain cadmium accumulation.

Sci Total Environ 2021 May 22;769:145345. Epub 2021 Jan 22.

Key Laboratory of Environmental Remediation and Ecosystem Health, Ministry of Education (MOE), College of Environmental and Resources Sciences, Zhejiang University, Hangzhou 310058, PR China. Electronic address:

To reduce cadmium (Cd) pollution of food chains, screening and breeding of low-Cd-accumulating genotypes have received increasing attention. However, the mechanisms involving Cd tolerance and accumulation are not fully understood. Here, we investigated the physiological responses and metabolomics profiling on two wheat (Triticum aestivum L.) genotypes, a low-Cd-accumulating genotype in grains (Aikang58, AK58) and a high-Cd-accumulating genotype in grains (Zhenmai10, ZM10), in hydroponic culture treated without/with Cd for 7 days. The results showed that AK58 was a Cd tolerant genotype with higher capacity of antioxidant systems in root. In addition, the concentrations of Cd bound to root cell walls were higher in AK58 than ZM10, of which pectin and hemicellulose played important roles in Cd binding. Moreover, subcellular distribution manifested that Cd sequestrated in the vacuoles was another tolerance mechanism in AK58. Simultaneously, metabolomics profiling showed that, in AK58, phenylalanine metabolism, alanine, aspartate and glutamate metabolism, isoquinoline alkaloid biosynthesis, arginine and proline metabolism, arginine biosynthesis and glyoxylate and dicarboxylate metabolism are highly related to antioxidant defense system, cell wall biosynthesis and metabolisms of phytochelatins together with other organic ligands, playing crucial roles in Cd tolerance and Cd fixation mechanisms in roots. These novel findings should be useful for molecular assisted screening and breeding of low Cd-accumulating genotypes for wheat crop.
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http://dx.doi.org/10.1016/j.scitotenv.2021.145345DOI Listing
May 2021

Variation of gut microbiota caused by an imbalance diet is detrimental to bugs' survival.

Sci Total Environ 2021 Jun 27;771:144880. Epub 2021 Jan 27.

State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan 430062, China. Electronic address:

Diet is an important factor in shaping and influencing both an insect's phenotype and gut bacterial community, which commonly establishes diversely symbiotic interactions with the host. Efforts to leverage the connection between diet, insects, and gut microbiome primarily focus on how diet alters insect's phenotype or gut microbial composition and relatively few studies have illuminated the link between the diet-induced insect phenotypic difference and variation of gut microbiota. Mirids bugs, Adelphocoris suturalis, are plant sap-feeding omnivores that sometimes complementarily prey on other insects, like aphids (the dietary regime is referred to hereafter as balanced diet). In this study, we found that an imbalanced diet (exclusive ingestion of aphids) induced significantly higher mortality in A. suturalis (86.66%). Further gut microbial community analysis showed that the dietary difference significantly changed both the abundance and composition of the bug's gut microbiome. Most notably, an abundance of entomopathogenic Serratia bacteria in the A. suturalis gut was positively correlated with the proportion of aphids in A. suturalis diet, and Serratia marcescens was found to transfer into the hemocoels of carnivorous bugs. Injection of S. marcescens to the hemocoels further confirmed its detrimental effect to the bugs. Collectively, our study suggests that the diet-altered variation of gut microbiota may be detrimental to host insect, advancing the knowledge of omnivorous insects' strategy in forage allocation of different foods.
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http://dx.doi.org/10.1016/j.scitotenv.2020.144880DOI Listing
June 2021

Phytoavailability, translocation and soil thresholds derivation of cadmium for food safety through soil-wheat (Triticum aestivum L.) system.

Environ Sci Pollut Res Int 2021 Mar 15. Epub 2021 Mar 15.

Key Laboratory of Environmental Remediation and Ecosystem Health, Ministry of Education (MOE), College of Environmental and Resources Sciences, Zhejiang University, Hangzhou, 310058, People's Republic of China.

Cadmium (Cd) pollution in cultivated soils has posed a great risk to human health through the soil-plant-human pathway. Therefore, it is important to derive soil thresholds for the low-Cd accumulating genotype of wheat (Triticum aestivum L.) to promote its application in agricultural production on Cd-contaminated sites. Here, a pot experiment was performed to explore the transfer characteristics of Cd in two contrasting wheat genotypes at three different soils and the effect of soil parameters together with soil safety Cd thresholds derivation. Generally, grain Cd highly accumulating wheat genotype (Zhenmai10, HT) showed higher Cd accumulation in grains than grain Cd weakly accumulating wheat genotype (Aikang58, LT). Stepwise multiple linear regression (SMLR) analysis (log-transformed Freundlich-type) indicated that Cd accumulation in wheat grains was strongly related to soil total Cd concentration and pH for both genotypes (R = 0.907*** for HT; R = 0.910*** for LT). Combining the simple regression model of soil-plant transfer system with the risk assessment method based on human health, soil total Cd thresholds for three soils were calculated with the values of 0.62, 0.82, and 0.62 mg kg in LT genotype and 0.31, 0.77, and 0.49 mg kg in HT genotype. Therefore, we suggested that when deducing soil thresholds, the ability of wheat genotypes to accumulate Cd and soil properties should be considered because of the large differences in soil thresholds between different genotypes and types of soils. We believe our results will promote the application of low-Cd wheat genotypes to agricultural production, thereby ensuring the safety of their products.
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http://dx.doi.org/10.1007/s11356-021-13385-9DOI Listing
March 2021

Metabolic and immunological effects of gut microbiota in leaf beetles at the local and systemic levels.

Integr Zool 2021 May 25;16(3):313-323. Epub 2021 Mar 25.

State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, China.

Insects' intestinal microbes have profound effects on the host's physiological traits, which can impact their physiology at both the local (gut) and systemic (body) levels. Nevertheless, the molecular mechanisms underlying host-microbiota interactions, especially in non-model insects, remain elusive. Recently, tissue-specific transcriptomic analysis has been highlighted as a robust tool in studying host-microbe interactions. Plagiodera versicolora is a worldwide leaf-eating pest that primarily feeds on willows and poplar. The interplay between gut microflora and this host beetle has yet to be studied. Herein, we investigate the effects of the gut microbiota on the body mass of P. versicolora larvae, compare the nutrition status of larvae in absence and presence of gut microbiota, and profile gut bacterial loads throughout its developmental larval stages. We then perform comparative transcriptomic analysis of gut and body tissues in axenic and non-axenic larvae. Finally, we confirm the expression patterns of representative genes in nutritional metabolism and immunity. Results show that weight growth is retarded in conventional larvae, with a concomitant increase of total bacterial load by the 5 development day, and germ-free larvae have a higher glucose content than conventional-reared larvae. Both nutritional and immunological analyses indicate that gut bacteria are a burden in the beetle's larval development. These findings elucidate the impacts of gut microbiota on P. versicolora, and provide insight into tissue-specific responses to gut microflora in this pest at the genetic level, boosting our understanding of the molecular mechanisms underlying host-microbe interactions in leaf beetles and beyond.
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http://dx.doi.org/10.1111/1749-4877.12528DOI Listing
May 2021

Modelling homosexual and heterosexual transmissions of hepatitis B virus in China.

J Biol Dyn 2021 Dec;15(1):177-194

School of Mathematics, Sichuan University, Chengdu, Sichuan, People's Republic of China.

Studies have shown that sexual transmission, both heterosexually and homosexually, is one of the main ways of HBV infection. Based on this fact, we propose a mathematical model to study the sexual transmission of HBV among adults by classifying adults into men and women and considering both same-sex and opposite-sex transmissions of HBV in adults. Firstly, we calculate the basic reproduction number and the disease-free equilibrium point . Secondly, by analysing the sensitivity of in terms of model parameters, we find that the infection rate among people who have same-sex partners, the frequency of homosexual contact and the immunity rate of adults play important roles in the transmission of HBV. Moreover, we use our model to fit the reported data in China and forecast the trend of hepatitis B. Our results demonstrate that popularizing the basic knowledge of HBV among residents, advocating healthy and reasonable sexual life style, reducing the number of adult carriers, and increasing the immunization rate of adults are effective measures to prevent and control hepatitis B.
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http://dx.doi.org/10.1080/17513758.2021.1896797DOI Listing
December 2021

Lead-Free Halide Perovskites for Light Emission: Recent Advances and Perspectives.

Adv Sci (Weinh) 2021 Feb 4;8(4):2003334. Epub 2021 Jan 4.

State Key Laboratory of Integrated Optoelectronics and College of Electronic Science and Engineering Jilin University Changchun 130012 China.

Lead-based halide perovskites have received great attention in light-emitting applications due to their excellent properties, including high photoluminescence quantum yield (PLQY), tunable emission wavelength, and facile solution preparation. In spite of excellent characteristics, the presence of toxic element lead directly obstructs their further commercial development. Hence, exploiting lead-free halide perovskite materials with superior properties is urgent and necessary. In this review, the deep-seated reasons that benefit light emission for halide perovskites, which help to develop lead-free halide perovskites with excellent performance, are first emphasized. Recent advances in lead-free halide perovskite materials (single crystals, thin films, and nanocrystals with different dimensionalities) from synthesis, crystal structures, optical and optoelectronic properties to applications are then systematically summarized. In particular, phosphor-converted LEDs and electroluminescent LEDs using lead-free halide perovskites are fully examined. Ultimately, based on current development of lead-free halide perovskites, the future directions of lead-free halide perovskites in terms of materials and light-emitting devices are discussed.
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http://dx.doi.org/10.1002/advs.202003334DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7887601PMC
February 2021

Cloning and expression of the ChGstα and ChGstκ genes in the gills of Crassostrea hongkongensis under nanoparticulate and ionic Zn stress.

Comp Biochem Physiol C Toxicol Pharmacol 2021 Jun 18;244:109007. Epub 2021 Feb 18.

Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Beibu Gulf University, Guangxi 535011, PR China. Electronic address:

Nanoparticulate and ionic Zn have potential impacts on the detoxification systems of organisms, and Gst genes play key roles in the detoxification of xenobiotics. In this study, we cloned the ChGstα and ChGstκ genes of C. hongkongensis, and studied their expression in gills under nanoparticulate and ionic Zn stress. The results showed that the coding sequences of the ChGstα and ChGstκ genes were 684 and 675 bp, respectively, and had no signal peptide; ChGstα was cytoplasmic, while ChGstκ was mitochondrial. The two genes were expressed in all 8 tested samples, with the most abundant expression observed in hemocytes for ChGstα and digestive glands for ChGstκ. After ZnCl or ZnoNP challenge, the expression of ChGstα decreased significantly in the ZnCl groups, and its expression was higher in the ZnoNP groups than in the ZnCl groups. The expression of ChGstκ was significantly decreased in the ZnCl and ZnoNP groups, and its expression was higher in the ZnoNP groups than in the ZnCl groups except at 3 h post metal Zn stress, which suggested that ChGstα and ChGstκ were more sensitive to ZnoNP than ZnCl.
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http://dx.doi.org/10.1016/j.cbpc.2021.109007DOI Listing
June 2021

Antimicrobial stewardship capacity and manpower needs in the Asia Pacific.

J Glob Antimicrob Resist 2021 Mar 3;24:387-394. Epub 2021 Feb 3.

Asia Pacific Foundation for Infectious Diseases, Seoul, Republic of Korea; CHA Bio Group, Gyeonggi-do, Republic of Korea.

Objectives: Antimicrobial stewardship is a strategy to combat antimicrobial resistance in hospitals. Given the burden and impact of antimicrobial resistance in the Asia Pacific, it is important to document capacity and gaps in antimicrobial stewardship programmes (ASP). We aimed to understand existing capacities and practices, and define the resources needed to establish antimicrobial stewardship where it is lacking.

Methods: An anonymous online survey, consisting of questions on antimicrobial control at country, hospital and programme levels, was circulated to healthcare providers in the field of infectious diseases and microbiology through Asian Network for Surveillance of Resistant Pathogens, ReAct Group and the Australasian Society for infectious Diseases.

Results: 139 participants from 16 countries or regions completed the survey. The majority of participants were adult infectious diseases physicians (61/139, 43.9%) and microbiologists (31/139, 22.3%). Participants from 7 countries reported that antimicrobials can be obtained without prescriptions. Despite the high percentage (75.5%) of respondents working in large hospitals, only 22/139 participants (15.8%) from Australia, China, Singapore, Taiwan, Thailand and Vietnam reported having more than 10 infectious diseases physicians. Hospital empiric antimicrobial guidelines for common infections were available according to 110/139 (79.1%) participants. Pre-authorisation of antimicrobials was reported by 88/113 (77.9%) respondents while prospective audit and feedback was reported by 93/114 (81.6%). Automatic stop orders and culture-guided de-escalation were reported by only 52/113 (46.0%) and 27/112 (24.1%) respectively.

Conclusion: The survey reveals a wide range of ASP development in Asia Pacific. Establishing national workgroups and guidelines will help advance antimicrobial stewardship in this diverse region.
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http://dx.doi.org/10.1016/j.jgar.2021.01.013DOI Listing
March 2021

MiR-133b inhibits colorectal cancer metastasis via lncRNA-LUCAT1.

Future Oncol 2021 Mar 5;17(9):1013-1023. Epub 2021 Feb 5.

Department of Gastrointestinal Surgery, The Third Xiangya Hospital of Central South University, Changsha, 410013, China.

Colorectal cancer (CRC) is a common malignant tumor of the digestive system. Metastasis is the leading cause of poor prognosis of CRC patients, warranting further study of the molecular mechanism of metastasis in CRC and identification of new therapeutic targets. MiR-133b has been proven to play an important role in tumorigenesis by directly targeting coding genes. However, whether miR-133b can regulate tumorigenesis via long noncoding RNA (lncRNA) remains unclear. We systematically analyzed the expression level and correlation of miR-133b and LUCAT1 in cancer tissues and adjacent tissues from 30 patients with CRC. The effects of miR-133b and LUCAT1 on the invasive ability of CRC cells were detected by a transwell assay. The relationship between miR-133b and LUCAT1 was investigated by cells transfection experiments, rescue experiments and luciferase reporter assays. The binding of LUCAT1 and EZH2 was detected by RNA immunoprecipitation assay. MiR-133b was expressed at low levels in CRC tissues, and LUCAT1 was highly expressed, with an inverse correlation between them. LUCAT1 promoted the migration and invasion of HCT116 and SW620 cells. Overexpression of LUCAT1 attenuated the inhibition of cell migration and invasion induced by miR-133b. However, the dual luciferase assay showed that miR-133b did not directly target LUCAT1. MiR-133b affects CRC metastasis via the LUCAT1/EZH2 complex. MiR-133b and LUCAT1 may be potential targets for antimetastasis therapy in CRC.
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http://dx.doi.org/10.2217/fon-2020-0420DOI Listing
March 2021

The electrogenic sodium bicarbonate cotransporter and its roles in the myocardial ischemia-reperfusion induced cardiac diseases.

Life Sci 2021 Apr 1;270:119153. Epub 2021 Feb 1.

Beijing Tiantan Hospital, Capital Medical University, Beijing 100070, China; Advanced Innovation Center for Human Brain Protection, Capital Medical University, Beijing 100070, China; National Clinical Research Center for Neurological Disease, Beijing 100070, China; School of Chemistry, Chemical Engineering and Life Science, Wuhan University of Technology, Wuhan 430070, China. Electronic address:

Cardiac tissue ischemia/hypoxia increases glycolysis and lactic acid accumulation in cardiomyocytes, leading to intracellular metabolic acidosis. Sodium bicarbonate cotransporters (NBCs) play a vital role in modulating intracellular pH and maintaining sodium ion concentrations in cardiomyocytes. Cardiomyocytes mainly express electrogenic sodium bicarbonate cotransporter (NBCe1), which has been demonstrated to participate in myocardial ischemia/reperfusion (I/R) injury. This review outlines the structural and functional properties of NBCe1, summarizes the signaling pathways and factors that may regulate the activity of NBCe1, and reviews the roles of NBCe1 in the pathogenesis of I/R-induced cardiac diseases. Further studies revealing the regulatory mechanisms of NBCe1 activity should provide novel therapeutic targets for preventing I/R-induced cardiac diseases.
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http://dx.doi.org/10.1016/j.lfs.2021.119153DOI Listing
April 2021