Publications by authors named "Min Jiang"

1,117 Publications

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A cryoprotectant supplemented with pentoxifylline can improve the effect of freezing on the motility of human testicular sperm.

Zygote 2021 Jun 22:1-6. Epub 2021 Jun 22.

Andrology/Human Sperm Bank, West China Second University Hospital, Sichuan University, Chengdu610041, China.

This study examined the effect of a cryoprotectant with and without pentoxifylline supplementation on the motility and viability of human testicular sperm, both before and after freezing. Testicular samples were obtained from 68 patients with azoospermia who came to the Andrology Service of West China Second University Hospital, Sichuan University, for testicular biopsies from December 2019 to April 2020. All patients were assigned randomly to two groups: experimental, whose testicular sperm were added to the cryoprotectant with pentoxifylline, and the control, whose testicular sperm were added to the cryoprotectant without pentoxifylline. Both groups used the same freezing and thawing methods. Testicular sperm motility in the experimental group was significantly higher than that of the control group, both before and after cryopreservation. The recovery rate of sperm motility in the experimental group was significantly higher than that of the control group. The percentage of samples with motile testicular sperm in the experimental group was significantly higher than that of the control group after thawing. Sperm viability was unchanged between the experimental and control groups, both before and after freezing. Overall, a pentoxifylline-supplemented cryoprotectant can significantly improve the motility of testicular sperm before and after cryopreservation.
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http://dx.doi.org/10.1017/S0967199421000368DOI Listing
June 2021

A cellular nitric oxide sensor based on porous hollow fiber with flow-through configuration.

Biosens Bioelectron 2021 Jun 16;191:113442. Epub 2021 Jun 16.

College of Pharmacy, Hangzhou Normal University, China; Key Laboratory of Elemene Class Anti-Cancer Medicines, Hangzhou Normal University, China. Electronic address:

Nitric oxide plays important transmission and regulation roles in the human body, but its in-vitro concentration is extremely low with a short half-life. In this work, we developed a three-dimensional 'flow-through' configuration based on polysulfone hollow fiber (PHF) for efficient detection of cell released NO. The PHF served as the substrate for cell culture as well as the base layer of the working electrode. The carbon nanotubes-gold nanoparticles (CNT-AuNPs) composites uniformly wrapped around the PHF as the sensing layer. The CNT provided a large specific surface area, which allowed uniform distribution and high loading of AuNPs, thus enhancing the electrocatalytic activity synergistically. Compared with the conventional flow-by configuration, such configuration resulted in a higher surface area per unit volume and enhanced NO molecule capture efficiency. The CNT-AuNPs PHF sensor showed a low detection limit (91 nM), high stability, selectivity, and biocompatibility. We utilized it for real-time in-situ detection of NO released by human lung cancer cell H1299 under drug stimulation. Furthermore, owing to the unique PHF structure, we performed long-term monitoring of NO release under the treatment of Lipopolysaccharide, Nitroglycerin and Aminoguanidine, which helps to understand the kinetic process of cellular drug response.
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http://dx.doi.org/10.1016/j.bios.2021.113442DOI Listing
June 2021

Changing incidence and projections of thyroid cancer in mainland China, 1983-2032: evidence from Cancer Incidence in Five Continents.

Cancer Causes Control 2021 Jun 21. Epub 2021 Jun 21.

Department of Epidemiology and Health Statistics, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, China.

Purpose: An increasing incidence of thyroid cancer has been seen in China during the past several decades. The aim of this study was to analyze potential age, period, and cohort effects on the incidence of thyroid cancer in mainland China and to predict new cases up to 2032.

Methods: We calculated age-adjusted and age-specific incidence rates of thyroid cancer, conducted an age-period-cohort analysis of 35,037 thyroid cancer incidence cases reported to Cancer Incidence in Five Continents from 1983 to 2012 in mainland China, and predicted incidence up to 2032 using the Bayesian age-period-cohort method.

Results: The age-adjusted overall incidence rate of thyroid cancer increased from 1.93/100,000 in 1983-1987 to 12.18/100,000 in 2008-2012 among females and from 0.77/100,000 in 1983-1987 to 3.89/100,000 in 2008-2012 among males, with a female-to-male ratio of approximately 3.0 during the three decades. Strong birth cohort and period effects on the incidence of thyroid cancer were observed for both sexes, and such an increasing trend is predicted to continue for at least the next 20 years. More than 3.7 million new cases are projected in the 2028-2032 period.

Conclusion: The increasing trend of thyroid cancer in mainland China will cause a great burden in the future. In addition to the potential impact of improvement in medical diagnostics, potential exposure to risk factors have played a role in the observed rising trend. Further population-based epidemiologic studies are required to identify risk factors to aid in thyroid cancer prevention and control.
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http://dx.doi.org/10.1007/s10552-021-01458-6DOI Listing
June 2021

Efficacy of simvastatin on carotid atherosclerotic plaque and its effects on serum inflammatory factors and cardiocerebrovascular events in elderly patients.

Exp Ther Med 2021 Aug 2;22(2):819. Epub 2021 Jun 2.

Department of Geriatrics, Baoji City People's Hospital, Baoji, Shaanxi 721000, P.R. China.

To investigate the efficacy of simvastatin on carotid atherosclerotic plaque (CAP) and its effects on serum inflammatory factors and cardiocerebrovascular events in elderly patients, 130 elderly patients with CAP were randomly divided into observation (n=65) and control groups (n=65). The control group was treated with 75 mg/day aspirin enteric-coated tablets, and the observation group was administered additional 20 mg/day simvastatin. Serum total cholesterol, triglyceride, and high- and low-density lipoprotein cholesterol levels (evaluated via the endpoint method) were determined in both groups. Furthermore, the length, thickness and number of CAPs was measured using color Doppler ultrasonography. In addition, levels of inflammatory biomarkers including high-sensitivity C-reactive protein (hs-CRP), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), nitric oxide, D-dimer and fibrinogen, as well as change in microemboli count, were also compared After treatment, the observation group exhibited a significant reduction in size, thickness, and number of CAP and intima-media thickness compared with before treatment. However, no significant difference was found in the indicators of CAPs in the control group before and after treatment. The levels of total cholesterol, triglyceride, and low-density lipid cholesterol decreased, while high-density lipid cholesterol increased in the observation group after treatment, with notable changes in the observation group compared with in the control group. Overall response rate was higher in the observation group compared with the control group. TNF-α, IL-6, and hs-CRP levels in the observation group decreased after treatment compared with those before treatment and those in the control group. Furthermore, the rate of microemboli positivity was lower in the observation group than in the control group. Moreover, the overall incidence of acute cardiocerebrovascular events was lower in the observation group than in the control group. Therefore, it was demonstrated that simvastatin can reduce blood lipid levels, decrease the quantity and size of plaques, alleviate inflammatory response, reduce microemboli formation and reduce the risk of cardiocerebrovascular events in elderly patients with CAP.
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http://dx.doi.org/10.3892/etm.2021.10251DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193215PMC
August 2021

Rapid and simple detection of in strawberry using a coupled recombinase polymerase amplification-lateral flow strip assay.

Phytopathol Res 2021 10;3(1):12. Epub 2021 Jun 10.

College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095 China.

is a devastating pathogen that infects a wide range of plants and causes Phytophthora rot disease, which has resulted in great economic losses in crop production. Therefore, the rapid and practicable detection of is important for disease monitoring and forecasting. In this study, we developed a lateral flow recombinase polymerase amplification (LF-RPA) assay for the sensitive visual detection of . Specific primers for were designed based on the ras-related protein gene ; all 10 isolates yielded positive detection results, whereas no cross-reaction occurred in related oomycete or fungal species. The detection limit for the LF-RPA assay was 100 fg of genomic DNA under optimized conditions. Combined with a simplified alkaline lysis method for plant DNA extraction, the LF-RPA assay successfully detected in naturally diseased strawberry samples without specialized equipment within 40 min. Thus, the LF-RPA assay developed in this study is a rapid, simple, and accurate method for the detection of , with the potential for further application in resource-limited laboratories.

Supplementary Information: The online version contains supplementary material available at 10.1186/s42483-021-00089-8.
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http://dx.doi.org/10.1186/s42483-021-00089-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8189726PMC
June 2021

Full composition granules of Huanglian (Rhizoma Coptidis) decrease the serum monocyte chemotactic protein-1 and connective tissue growth factor levels and inhibit kidney nuclear factor-κB expression in rats with high-fat diet-induced diabete.

J Tradit Chin Med 2021 Jun;41(3):424-431

Department of Traditional Chinese Medicine, the Mongolian Medical and Traditional Chinese Medicine Hospital of Wuhai, Wuhai 016000, China.

Objective: To investigate the efficacy of the full composition granules of Huanglian (Rhizoma Coptidis) (FGC) on the serum monocyte chemotactic protein-1 (MCP-1) and connective tissue growth factor (CTGF) levels and kidney nuclear factor-κB (NF-κB) expression in rats with high-fat diet-induced diabetes.

Methods: Diabetes was induced in rats by feeding a high-fat chow combined with intravenous streptozotocin injection. Forty diabetic Sprague- Dawley rats were randomly assigned to a normal group (NG), model group (MG), irbesartan group (IG), and low-, middle-, and high-dosage FGC groups (LFGC, MFGC, HFGC), with eight rats per group. The IG rats received 31.25 mg·kg-1·d-1 irbesartan tablets, whereas those in the LFGC, MFGC, and HFGC were administered 52, 312.5, and 625 mg·kg-1·d-1 FGC, respectively. After 12 weeks, bodyweight (BW), left kidney weight (KW), hemoglobin A1c (HbA1c), serum creatinine (Scre), blood urea nitrogen (BUN), and serum MCP-1 and CTGF levels were determined, pathological changes of the kidney were recorded, and kidney NF-κB p65 (A) expression was measured.

Results: The 24-h urine albumin and levels of HbA1c, Scre, BUN, and serum MCP-1 and CTGF were significantly increased in in the MG compared with the NG, as was the kidney NF-κB (p65) expression (P < 0.05). Furthermore, clear pathological changes in kidney fibrosis were observed in the MG rats. Following irbesartan and FGC administration, the 24-h urine albumin and the levels of HbA1c, Scre, and serum MCP-1 and CTGF were significantly decreased in FCG groups compared with those in the MG, which is in agreement with the change in the kidney NF-κB (p65) expression, whereas the similarly significant decrease only exist in 24-h urine albumin and the levels of serum CTGF after irbesartan administration. Hematoxylin-eosin (HE) staining results indicated that the fibrosis observed in the MG samples was alleviated through FGC treatment.

Conclusion: FGC may alleviate potential kidney injury by decreasing the serum MCP-1 and CTGF levels and inhibiting NF-kB expression in diabetic nephropathy in rats with high-fat diet-induced diabetes.
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http://dx.doi.org/10.19852/j.cnki.jtcm.2021.03.010DOI Listing
June 2021

Early Prediction Model of Gestational Hypertension by Multi-Biomarkers Before 20 Weeks Gestation.

Diabetes Metab Syndr Obes 2021 31;14:2441-2451. Epub 2021 May 31.

Department of Laboratory, Guangzhou Women and Children's Medical Centre, Guangzhou Medical University, Guangzhou, 510623, People's Republic of China.

Background: Gestational hypertension (GH), a hypertensive disorder of pregnancy (HDP), is a leading cause of maternal and fetal mortality due to the lack of clarity on its exact etiology and clinically feasible prediction models. This study was performed to discover novel biomarkers before 20 weeks gestation and thereby construct an early GH prediction model.

Methods: This study was designed based on differentially expressed protein screening followed by clinical validation. In the screening phase, a nested case-controlled study was conducted by plasma proteomic analyses using label-free LC-MS/MS and plasma samples from seven pre-GH cases before 20-week gestation and seven age- and gestational week-matched controls. In the validation phase, 10 proteins with differential expression in the screening phase were validated by ELISA or electrochemiluminescence in an independent study consisting of 29 pre-GH cases before 20-week gestation and 29 matched controls.

Results: In the screening phase, 149 proteins were found to be differentially expressed between the two groups and were predominantly involved in complement and coagulation cascades, platelet degranulation and positive regulation of cell motility. Further validation showed that serpin family C member 1 (SERPINC1), serpin family A member 5 (SERPINA5), complement factor H-related protein 5 (CFHR5), clusterin, cytokeratin 18 (CK18) and histidine-rich glycoprotein (HRG) levels were significantly higher in women who later developed GH compared to women with uncomplicated pregnancies (<0.05). Binary logistic regression analysis was used to determine the combination efficacy of models for early prediction of GH. The model with a combination of SERPINC1, CK18 and HRG had a significantly better discriminatory power (AUC = 0.91, 95% CI 0.83-0.98) compared to the models with those proteins alone as independent predictors of GH.

Conclusion: Plasma levels of SERPINC1, SERPINA5, CFHR5, clusterin, CK18 and HRG are potential novel predictive biomarkers of GH, and a prediction model using a combination of SERPINC1, CK18 and HRG has good discriminatory performance for GH before 20 weeks gestation.
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http://dx.doi.org/10.2147/DMSO.S309725DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8178612PMC
May 2021

A Thrombin-Responsive Nanoprobe for Visualization of Thrombus Formation through Three-Dimensional Optical/Computed Tomography Hybrid Imaging.

ACS Appl Mater Interfaces 2021 Jun 8. Epub 2021 Jun 8.

Department of Cardiology &National Clinical Research Center of Geriatric Disease, 2nd Medical Center of Chinese PLA General Hospital, Beijing 100853, China.

Early spontaneous detection of thrombin activation benefits precise theranostics for thrombotic vascular disease. Herein, a thrombin-responsive nanoprobe conjugated by a FITC dye, PEGylated FeO nanoparticles, and a thrombin-sensitive peptide (LASG) was constructed to visualize thrombin activation and subsequent thrombosis . The FITC dye was linked to the LASG coated on the FeO nanoparticles for sensing the thrombin activity via the Förster resonance energy transfer effect. fluorescence imaging showed that the fluorescence signal intensity increased significantly after incubation with thrombin in contrast to that of the control group ( < 0.05), and the signal intensity was enhanced with the increase in thrombin concentration. Further fluorescence imaging also revealed that the signal elevated markedly in the left common carotid artery (LCCA) lesion of the mice thrombosis model after nanoprobe injection, in contrast to that of the control + nanoprobe group ( < 0.05). Moreover, the thrombin inhibitor bivalirudin could decrease the filling defect of the LCCA. Three-dimensional fusion images of micro-CT and fluorescence confirmed that filling defects in the LCCA were nicely colocalized with fluorescence signal caused by nanoprobes. The nanoplatform based on a thrombin-activatable visualization system could provide smart responsive and dynamic imaging of thrombosis .
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http://dx.doi.org/10.1021/acsami.1c04065DOI Listing
June 2021

[Advances in the metabolic engineering for the production of tetracarbon organic acids].

Sheng Wu Gong Cheng Xue Bao 2021 May;37(5):1697-1720

State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, Jiangsu, China.

Tetracarbon organic acids are important platform chemicals that are widely used in the food, chemical, medicine, material industries and agriculture. Compared with the traditional petrochemical process, the production of tetracarbon organic acids by microbial fermentation is more promising due to milder reaction conditions, greener process and better environmental compatibility. This review summarizes the biosynthetic pathways and metabolic mechanisms for the production of tetracarbon organic acids, and illustrates recent advances, challenges, and future perspectives in the production of tetracarbon organic acids by naturally selected or purposefully engineered strains.
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http://dx.doi.org/10.13345/j.cjb.200727DOI Listing
May 2021

The Efficacy and Safety of Fractional 1064 nm Nd:YAG Picosecond Laser Combined With Intense Pulsed Light in the Treatment of Atrophic Acne Scar: A Split-Face Study.

Lasers Surg Med 2021 Jun 3. Epub 2021 Jun 3.

Department of Dermatology, Huashan Hospital, Shanghai Medical College, Fudan University, 12 Wulumuqi Road, Shanghai, 200040, China.

Background And Objectives: To evaluate the efficacy and safety of fractional 1064 nm Nd:YAG picosecond laser combined with intense pulsed light (IPL) in the treatment of atrophic acne scar with post-inflammatory erythema (PIE). © 2021 Wiley Periodicals LLC.

Study Design/materials And Methods: Seventeen patients received five sessions of treatment at weeks 0, 4, 8, 12, 16 and were followed up at week 28. One half of the face was randomly treated by fractional 1064 nm Nd:YAG picosecond laser combined with IPL (FxPico + IPL), and the other by IPL alone as a control.

Results: For the 15 patients who completed the study, the FxPico + IPL side demonstrated significant median Échelle D'évaluation clinique des cicatrices D'acné (ECCA) score improvement (P < 0.01), while IPL alone side did not (P = 0.1250). The pore counts for both sides decreased but more pore count reduction was seen on the FxPico + IPL side (P < 0.05). Better scar improvement was observed on the FxPico + IPL-treated side (P < 0.05) while no difference in erythema improvement was seen between the two sides. There was no difference between the two treatments in terms of overall satisfaction. Pain, erythema, edema, petechiae, crusting, reactive acneiform eruptions, and pruritus were common adverse effects and were mild to moderate.

Conclusion: FxPico + IPL is an effective, efficient, and safe treatment regimen for atrophic acne scars complicated by PIE.
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http://dx.doi.org/10.1002/lsm.23428DOI Listing
June 2021

Impact of treatment delays on vitiligo during the COVID-19 pandemic: A retrospective study.

Dermatol Ther 2021 Jun 1:e15014. Epub 2021 Jun 1.

Department of Dermatology, Huashan Hospital, Fudan University, Shanghai, China.

Due to the COVID-19 pandemic, routine treatments are delayed to some extent and their negative impacts have been widely reported. However, virtually nothing is known about vitiligo in the context of COVID-19. Therefore, we analyzed treatment delays and its impact on vitiligo, aiming to provide suggestions on vitiligo management within this special period. We performed a retrospective cohort study on 322 patients who visited our clinics at least 2 times from January to December 2020, and their medical records and photographs were reviewed. Patients were divided into normal (n = 155) and late group (n = 167) based on whether experienced treatment delays. As for the active cases, the late group showed higher progression rate than normal group (35 of 86 [40.7%] vs. 10 of 81 [12.3%]; p = 0.002). Moreover, we observed higher recurrence rate in delay group than those of normal group (26 of 81[32.1%] vs. 9 of 74 [12.2%]; p = 0.018) among stable cases. Further univariate and multivariate analysis determined treatment delays as the most important independent risk factor for disease progression and recurrence, and maintenance therapy (>2 years) as a protective factor against recurrence. This study, for the first time, revealed the independent adverse impact of treatment delays on the progression and recurrence of vitiligo and indicated the significance of continuous treatment for halting progression and long-term maintenance therapy for preventing recurrence for vitiligo, which should be highly valued in the management of vitiligo during the COVID-19 pandemic.
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http://dx.doi.org/10.1111/dth.15014DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8209886PMC
June 2021

Effect of Digestate and Straw Combined Application on Maintaining Rice Production and Paddy Environment.

Int J Environ Res Public Health 2021 May 26;18(11). Epub 2021 May 26.

Jiangsu Key Laboratory of Crop Genetics and Physiology/Jiangsu Key Laboratory of Crop Cultivation and Physiology, Agricultural College of Yangzhou University, Yangzhou 225009, China.

Few studies have focused on the combined application of digestate and straw and its feasibility in rice production. Therefore, we conducted a two-year field experiment, including six treatments: without nutrients and straw (Control), digestate (D), digestate + fertilizer (DF), digestate + straw (DS), digestate + fertilizer + straw (DFS) and conventional fertilizer + straw (CS), to clarify the responses of rice growth and paddy soil nutrients to different straw and fertilizer combinations. Our results showed that digestate and straw combined application (i.e., treatment DFS) increased rice yield by 2.71 t ha compared with the Control, and digestate combined with straw addition could distribute more nitrogen (N) to rice grains. Our results also showed that the straw decomposition rate at 0 cm depth under DS was 5% to 102% higher than that under CS. Activities of catalase, urease, sucrase and phosphatase at maturity under DS were all higher than that under both Control and CS. In addition, soil organic matter (SOM) and total nitrogen (TN) under DS and DFS were 20~26% and 11~12% higher than that under B and DF respectively, suggesting straw addition could benefit paddy soil quality. Moreover, coupling straw and digestate would contribute to decrease the N content in soil surface water. Overall, our results demonstrated that digestate and straw combined application could maintain rice production and have potential positive paddy environmental effects.
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http://dx.doi.org/10.3390/ijerph18115714DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8197978PMC
May 2021

Characterization of Reveals Its Role in Carotenoid Biosynthesis in Chinese Kale.

Front Plant Sci 2021 14;12:662684. Epub 2021 May 14.

College of Horticulture, Sichuan Agricultural University, Chengdu, China.

Carotenoids are organic pigments that play an important role in both plant coloration and human health; they are a critical subject in molecular breeding due to growing demand for natural molecules in both food and medicine. In this study, we focus upon characterizing , the carotenoid isomerase gene before the branch of the carotenoid biosynthetic pathway, which is expressed in all organs and developmental stages of Chinese kale, and BoaCRTISO, which is located in the chloroplast. The expression of is induced by strong light, red and blue combined light, and gibberellic acid treatment, but it is suppressed by darkness and abscisic acid treatment. We obtained -silenced plants via virus-induced gene silencing technology, and the silence efficiencies ranged from 52 to 77%. The expressions of most carotenoid and chlorophyll biosynthetic genes in -silenced plants were downregulated, and the contents of carotenoids and chlorophyll were reduced. Meanwhile, -silenced plants exhibited phenotypes of yellowing leaves and inhibited growth. This functional characterization of provides insight for the biosynthesis and regulation of carotenoid in Chinese kale.
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http://dx.doi.org/10.3389/fpls.2021.662684DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8160315PMC
May 2021

Application of the health action process approach model for reducing excessive internet use behaviors among rural adolescents in China: a school-based intervention pilot study.

BMC Public Health 2021 05 26;21(1):986. Epub 2021 May 26.

Department of Health Behavior and Social Medicine, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, China.

Objective: There are few studies regarding Internet use behaviors of Chinese rural adolescents based on behavioral theory. The aim of this study is to examine the applicability and effectiveness of the health action process approach model (HAPA) in the intervention of excessive Internet use behaviors among rural adolescents in China.

Methods: Three hundred twenty-seven participants who met the excessive Internet use criteria were involved in this study. Four interventions based on the HAPA model were conducted during 2015-2017. The structural equation model (SEM) was applied to fit the HAPA model.

Results: The rate of average daily time spent online on weekends more than 4 h dropped from 57.2 to 39.1% (P < 0.001). The rate of daily game time more than 2 h decreased from 51.1 to 35.2% (P < 0.001). The result of SEM showed that both the applicability and effectiveness of the HAPA model were well in the intervention of excessive Internet use behaviors with good fitted indicators (χ/df = 2.066, GFI = 0.889, CFI = 0.938, TLI = 0.928, IFI = 0.938, RMSEA = 0.057). The direct and indirect effects of the main pathways in the HAPA model were statistically significant (P < 0.05). The comparison analysis of HAPA model variables identified that outcome expectancy, intention, maintenance self-efficacy had been improved significantly after interventions.

Conclusion: The intervention measures based on the HAPA model can effectively reduce excessive Internet use behaviors of Chinese rural adolescents, mainly through strengthen outcome expectancy, intention, and maintenance self-efficacy.
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http://dx.doi.org/10.1186/s12889-021-10999-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8152115PMC
May 2021

Inverse Gaussian Process Modeling for Evolutionary Dynamic Multiobjective Optimization.

IEEE Trans Cybern 2021 May 25;PP. Epub 2021 May 25.

For dynamic multiobjective optimization problems (DMOPs), it is challenging to track the varying Pareto-optimal front. Most traditional approaches estimate the Pareto-optimal sets in the decision space. However, the obtained solutions do not necessarily satisfy the desired properties of decision makers in the objective space. Inverse model-based algorithms have a great potential to solve such problems. Nonetheless, the existing ones have low precision for handling DMOPs with nonlinear correlations between the objective and decision vectors, which greatly limits the application of the inverse models. In this article, an inverse Gaussian process (IGP)-based prediction approach for solving DMOPs is proposed. Unlike most traditional approaches, this approach exploits the IGP to construct a predictor that maps the historical optimal solutions from the objective space to the decision space. A sampling mechanism is developed for generating sample points in the objective space. Then, the IGP-based predictor is employed to generate an effective initial population by using these sample points. The proposed method by introducing IGP can obtain solutions with better diversity and convergence in the objective space, which is more responsive to the demand of decision makers than the traditional methods. It also has better performance than other inverse model-based methods in solving nonlinear DMOPs. To investigate the performance of the proposed approach, experiments have been conducted on 23 benchmark problems and a real-world raw ore allocation problem in mineral processing. The experimental results demonstrate that the proposed algorithm can significantly improve the dynamic optimization performance and has certain practical significance for solving real-world DMOPs.
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http://dx.doi.org/10.1109/TCYB.2021.3070434DOI Listing
May 2021

High expression of guanine nucleotide-binding protein-like-3-like is associated with poor prognosis in esophageal cancer.

Medicine (Baltimore) 2021 May;100(21):e25993

Department of Oncology, Taizhou People's Hospital, Nanjing University of Traditional Chinese Medicine, Taizhou, Jiangsu, China.

Abstract: Guanine nucleotide-binding protein-like-3-like (GNL3L) is required for processing ribosomal pre-rRNA and cell proliferation and is upregulated in many types of cancer. This study is aimed to investigate the clinical significance of GNL3L in esophageal cancer. The mRNA and protein expression levels of GNL3L were determined by using quantitative real-time polymerase chain reaction and immunohistochemistry, respectively. GNL3L was localized in both cytoplasm and nucleus. The expression levels of GNL3L in esophageal cancer tissues were significantly higher than those in adjacent nonmalignant tissues. High GNL3L expression was associated with pathologic type and poor differentiation. Patients with high GNL3L expression had shorter overall survival (OS) than those with low GNL3L expression. Multivariate Cox regression analysis revealed that GNL3L expression was an independently predictive factor for the OS of patient with esophageal cancer. The Gene Expression Profiling Interactive Analysis (GEPIA) databases also showed that GNL3L was upregulated in esophageal cancer, which was closely associated with an unfavorable prognosis of patients with esophageal cancer. Taken together, our findings suggest that GNL3L is upregulated in esophageal cancer, which is linked to the progression of the disease. As a result, GNL3L could be used as a biomarker for esophageal cancer.
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http://dx.doi.org/10.1097/MD.0000000000025993DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8154413PMC
May 2021

[Micro-morphological Characteristics of Particles on Holly and Ligustrum Leaf Surfaces and Seasonal Changes in Bacterial Communities].

Huan Jing Ke Xue 2021 Jun;42(6):3063-3073

Institute of Environment and Municipal Engineering, Qingdao University of Technology, Qingdao 266033, China.

The characteristics of particles and microorganisms on leaf surfaces have great significance for the near-surface environment and ecology. Here, fresh leaves of holly and Ligustrum were examined from different functional areas in Qingdao. Environmental scanning electron microscopy was used to observe particles and microorganisms on the leaf surfaces during different seasonal, and Illumina high-throughput sequencing was performed to analyze the phyllosphere bacterial community structure. The results showed that the retention of TSP on leaves in autumn and winter was higher than in spring and summer. The leaves of the two plants were more likely to retain PM and PM; however, the dust retention capacity of holly leaves was higher than that of Ligustrum, especially for particle sizes greater than 10 μm. The numbers of particles on the leaf surfaces along an urban main road were higher than in two other locations, and were greatly affected by ground dust and automobile exhaust emissions. The community structure of phyllospheric microorganisms showed distinct seasonal variation, with different types of fungi, fungal spores, and mycelium observed on the leaf surfaces. Mycelium was more frequently detected on leathery leaves, and fungal spores with even folds were detected on the leaf epidermis of Ligustrum. The relative abundances of phyllospheric microorganisms were highest on leaves from an urban park, explained by a positive effect of humidity on growth. Significant differences in bacterial community abundance were observed between seasons. Specifically, bacterial abundance was highest in spring and lowest in summer. γ-Proteobacteria were the dominant bacteria, and the two plants shared a similar core microbial community. In addition, the phyllospheric bacterial community structure of leaves from urban arterial roads with ground dust pollution was significantly different from the leaves collected from other city areas. Our research results suggest a significant correlation between the leaf-surface particles and microbial community structure on representative plants in different areas of the city, which provides reference information for urban greening activities.
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http://dx.doi.org/10.13227/j.hjkx.202010220DOI Listing
June 2021

Comparative evaluation of six nucleic acid amplification kits for SARS-CoV-2 RNA detection.

Ann Clin Microbiol Antimicrob 2021 May 22;20(1):38. Epub 2021 May 22.

Shenzhen Center for Disease Control and Prevention, 8 Longyuan Road, Nanshan District, Shenzhen, 518055, China.

Background: SARS-CoV-2 is a newly emerged coronavirus, causing the coronavirus disease 2019 (COVID-19) outbreak in December, 2019. As drugs and vaccines of COVID-19 remain in development, accurate virus detection plays a crucial role in the current public health crisis. Quantitative real-time reverse transcriptase-polymerase chain reaction (RT-qPCR) kits have been reliably used for detection of SARS-CoV-2 RNA since the beginning of the COVID-19 outbreak, whereas isothermal nucleic acid amplification-based point-of-care automated kits have also been considered as a simpler and rapid alternative. However, as these kits have only been developed and applied clinically within a short timeframe, their clinical performance has not been adequately evaluated to date. We describe a comparative study between a newly developed cross-priming isothermal amplification (CPA) kit (Kit A) and five RT-qPCR kits (Kits B-F) to evaluate their sensitivity, specificity, predictive values and accuracy.

Methods: Fifty-two clinical samples were used including throat swabs (n = 30), nasal swabs (n = 7), nasopharyngeal swabs (n = 7) and sputum specimens (n = 8), comprising confirmed (n = 26) and negative cases (n = 26). SARS-CoV-2 detection was simultaneously performed on each sample using six nucleic acid amplification kits. The sensitivity, specificity, positive/negative predictive values (PPV/NPV) and the accuracy for each kit were assessed using clinical manifestation and molecular diagnoses as the reference standard. Reproducibility for RT-qPCR kits was evaluated in triplicate by three different operators using a SARS-CoV-2 RNA-positive sample. On the basis of the six kits' evaluation results, CPA kit (Kit A) and two RT-qPCR Kits (Kit B and F) were applied to the SARS-CoV-2 detection in close-contacts of COVID-19 patients.

Results: For Kit A, the sensitivity, specificity, PPV/NPV and accuracy were 100%. Among the five RT-qPCR kits, Kits B, C and F had good agreement with the clinical diagnostic reports (Kappa ≥ 0.75); Kits D and E were less congruent (0.4 ≤ Kappa < 0.75). Differences between all kits were statistically significant (P < 0.001). The reproducibility of RT-qPCR kits was determined using a coefficients of variation (CV) between 0.95% and 2.57%, indicating good reproducibility.

Conclusions: This is the first comparative study to evaluate CPA and RT-qPCR kits' specificity and sensitivity for SARS-CoV-2 detection, and could serve as a reference for clinical laboratories, thus informing testing protocols amid the rapidly progressing COVID-19 pandemic.
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http://dx.doi.org/10.1186/s12941-021-00443-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8140580PMC
May 2021

Mercapturate pathway metabolites of sotorasib, a covalent inhibitor of KRAS, are associated with renal toxicity in the Sprague Dawley rat.

Toxicol Appl Pharmacol 2021 07 15;423:115578. Epub 2021 May 15.

Amgen Research, Translational Safety and Bioanalytical Sciences, Thousand Oaks, CA, USA.

Sotorasib is a first-in class KRAS covalent inhibitor in clinical development for the treatment of tumors with the KRAS p.G12C mutation. In the nonclinical toxicology studies of sotorasib, the kidney was identified as a target organ of toxicity in the rat but not the dog. Renal toxicity was characterized by degeneration and necrosis of the proximal tubular epithelium localized to the outer stripe of the outer medulla (OSOM), which suggested that renal metabolism was involved. Here, we describe an in vivo mechanistic rat study designed to investigate the time course of the renal toxicity and sotorasib metabolites. Renal toxicity was dose- and time-dependent, restricted to the OSOM, and the morphologic features progressed from vacuolation and necrosis to regeneration of tubular epithelium. The renal toxicity correlated with increases in renal biomarkers of tubular injury. Using mass spectrometry and matrix-assisted laser desorption/ionization, a strong temporal and spatial association between renal toxicity and mercapturate pathway metabolites was observed. The rat is reported to be particularly susceptible to the formation of nephrotoxic metabolites via this pathway. Taken together, the data presented here and the literature support the hypothesis that sotorasib-related renal toxicity is mediated by a toxic metabolite derived from the mercapturate and β-lyase pathway. Our understanding of the etiology of the rat specific renal toxicity informs the translational risk assessment for patients.
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http://dx.doi.org/10.1016/j.taap.2021.115578DOI Listing
July 2021

Ginsenoside 20(S)-Rg3 suppresses cell viability in esophageal squamous cell carcinoma via modulating miR-324-5p-targeted PSME3.

Hum Exp Toxicol 2021 May 18:9603271211017311. Epub 2021 May 18.

Department of Pathology, Taizhou People's Hospital Affiliated to Nanjing University of Chinese Medicine, Taizhou, Jiangsu, China.

Ginsenoside 20(S)-Rg3 is identified as an active saponin monomer which derived from red ginseng and is demonstrated to play an anti-tumor role in diverse cancers. MicroRNAs (miRNAs) are important regulators in the progression of cancers, containing esophageal squamous cell carcinoma (ESCC). It was reported that microRNA 324-5p (miR-324-5p) exerted critical functions in some cancers; however, the detailed molecular mechanism of miR-324-5p mediated by 20(S)-Rg3 to suppress cell viability in ESCC has not been explored. Herein, we explored the function of 20(S)-Rg3 or miR-324-5p on ESCC cell viability by MTT assay, colony formation assay, flow cytometry analysis and western blot analysis. The binding of miR-324-5p to its target gene, proteasome activator subunit 3 (PSME3), was confirmed through RNA pull down and luciferase reporter assays. The results indicated that 20(S)-Rg3 significantly inhibited cell viability and the cell cycle and facilitated cell apoptosis. Furthermore, this effect was strengthened with the increased concentration of 20(S)-Rg3. Moreover, we found that miR-324-5p level was increased under 20(S)-Rg3 treatment. Additionally, overexpressed miR-324-5p inhibited ESCC cell viability, and downregulated miR-324-5p recovered inhibited cell viability caused by 20(S)-Rg3. Further exploration verified that miR-324-5p targeted PSME3, and PSME3 deficiency countervailed the effect of miR-324-5p inhibition on ESCC cell viability under 20(S)-Rg3 treatment. Conclusively, 20(S)-Rg3 suppresses cell viability in ESCC via mediating miR-324-5p-targeted PSME3.
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http://dx.doi.org/10.1177/09603271211017311DOI Listing
May 2021

Identification of a novel bluetongue virus 1 specific B cell epitope using monoclonal antibodies against the VP2 protein.

Int J Biol Macromol 2021 May 11;183:1393-1401. Epub 2021 May 11.

School of Life Sciences, Zhengzhou University, Zhengzhou 450001, China. Electronic address:

Bluetongue (BT) is a non-contact infectious disease caused by Bluetongue virus (BTV), which can be transmitted by vector insects such as Culicoides and Aedes mosquitoes. The BTV VP2 protein encoded by the L2 gene is located at the outermost layer of the virus particle, plays a key role on mediating the adsorption and entry of virus, and it is also a main antigenic protein widely used for vaccine development. In this study, the BTV1 VP2 gene was cloned into pFastBac™Dual vector, and expressed in insect Sf21 cells. Immunized mice with purified recombinant VP2 protein can induce higher levels of antibodies. Three anti BTV1 VP2 monoclonal antibodies (mAbs) were generated (17E9C6, 17E9C8, 17E9H12), and showed high specific reactivity with recombinant VP2 protein and inactivated BTV1 virus. Finally, a novel linear B-cell epitope KEPAD on recombinant VP2 protein was identified by using three mAbs react with a series of continue-truncated peptides. The results of this study may provide new information on the structure and function of BTV1 VP2 protein and lay a foundation for the development of BTV1 diagnostic and prophylactic methods.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.05.053DOI Listing
May 2021

Efficacy and Safety of Sea Salt-Derived Physiological Saline Nasal Spray as Add-On Therapy in Patients with Acute Upper Respiratory Infection: A Multicenter Retrospective Cohort Study.

Med Sci Monit 2021 May 11;27:e929714. Epub 2021 May 11.

Department of Respiratory and Critical Care Medicine, Jiangxi Provincial People's Hospital Affiliated to Nanchang University, Nanchang, Jiangxi, China (mainland).

BACKGROUND The purpose of this study was to assess the effects of seawater on nasal congestion and runny nose symptoms in adults with an acute upper respiratory infection (URI). MATERIAL AND METHODS This was a multicenter retrospective cohort trial of patients with acute URI and symptoms of nasal congestion and runny nose. The patients were assigned to 2 groups and were administered regular non-drug supportive treatment or supportive treatment with nasal irrigation with sea salt-derived physiological saline. The primary efficacy endpoint was the effective rate (percentage of patients with ≥30% symptom score reduction from baseline for nasal congestion and runny nose). RESULTS In total, 144 patients were enrolled, including 72 in each group, and 143 patients completed the study. Both groups had similar demographics and vital signs. The effective rates for nasal congestion and runny nose were significantly increased in the seawater group compared with patients in the control group (87.3% vs 59.7% for nasal congestion; 85.9% vs 61.1% for runny nose; both P<0.001). In addition, the 2 groups showed markedly different degrees of patient symptom score improvement in sleep quality and appetite (both P<0.01), but not in cough and fatigue (both P>0.05). There were no adverse events in either group. CONCLUSIONS The sea salt-derived physiological saline nasal spray device satisfactorily improved nasal congestion, runny nose, sleep quality, and appetite in adults with URI, with no adverse effects.
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http://dx.doi.org/10.12659/MSM.929714DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8122848PMC
May 2021

FMRP regulates STAT3 mRNA localization to cellular protrusions and local translation to promote hepatocellular carcinoma metastasis.

Commun Biol 2021 May 10;4(1):540. Epub 2021 May 10.

Cancer Metastasis Alert and Prevention Center, Fujian Provincial Key Laboratory of Cancer Metastasis Chemoprevention and Chemotherapy, National & Local Joint Biomedical Engineering Research Center on Photodynamic Technologies, State Key Laboratory of Photocatalysis on Energy and Environment, College of Chemistry, Fuzhou University, Fuzhou, China.

Most hepatocellular carcinoma (HCC)-associated mortalities are related to the metastasis of cancer cells. The localization of mRNAs and their products to cell protrusions has been reported to play a crucial role in the metastasis. Our previous findings demonstrated that STAT3 mRNA accumulated in the protrusions of metastatic HCC cells. However, the underlying mechanism and functional significance of this localization of STAT3 mRNA has remained unexplored. Here we show that fragile X mental retardation protein (FMRP) modulates the localization and translation of STAT3 mRNA, accelerating HCC metastasis. The results of molecular analyses reveal that the 3'UTR of STAT3 mRNA is responsible for the localization of STAT3 mRNA to cell protrusions. FMRP is able to interact with the 3'UTR of STAT3 mRNA and facilitates its localization to protrusions. Importantly, FMRP could promote the IL-6-mediated translation of STAT3, and serine 114 of FMRP is identified as a potential phosphorylation site required for IL-6-mediated STAT3 translation. Furthermore, FMRP is highly expressed in HCC tissues and FMRP knockdown efficiently suppresses HCC metastasis in vitro and in vivo. Collectively, our findings provide further insights into the mechanism of HCC metastasis associated with the regulation of STAT3 mRNA localization and translation.
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http://dx.doi.org/10.1038/s42003-021-02071-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8110961PMC
May 2021

Downregulation of GLYAT Facilitates Tumor Growth and Metastasis and Poor Clinical Outcomes Through the PI3K/AKT/Snail Pathway in Human Breast Cancer.

Front Oncol 2021 22;11:641399. Epub 2021 Apr 22.

Department of Oncology, Shengjing Hospital of China Medical University, Shenyang, China.

Background: The Glycine N-acyltransferase (GLYAT) gene encodes a protein that catalyzes the transfer of acyl groups from acyl CoA to glycine, resulting in acyl glycine and coenzyme A. Aberrant GLYAT expression is associated with several malignant tumors, but its clinical importance in human breast cancer (BC), has yet to be fully addressed. This study aims to evaluate the clinical function of GLYAT in BC patients.

Methods: GLYAT expression was determined by immune blot and immunohistochemistry in three BC cell lines and primary cancer tissues. The MDA-MB 231 cell line was used for GLYAT gene knockdown experiments while the MCF7 cell line for overexpression experiments. Colony formation experiments, soft agar experiments, and transwell assays were utilized for further inspection of cell proliferation and migration capabilities. Immunofluorescence and western blot were used to detect markers of the epithelial-mesenchymal transition (EMT) and changes in the PI3K/AKT/Snail pathway. The role of GLYAT in tumor growth and metastasis was also assessed in nude mice . Also, a correlation analysis was performed between clinicopathological features and GLYAT expression in BC patients.

Results: GLYAT was decreased in human BC tissues and cell lines. Functional analysis showed that knockdown of GLYAT augmented BC cell proliferation and . However, this phenomenon was reversed when GLYAT was overexpressed in the transfected cells. Moreover, downregulation of GLYAT promoted the migratory properties of BC cells, likely through the activation of PI3K/AKT/Snail signaling, which subsequently induced the EMT. IHC analysis indicated that GLYAT was decreased in human BC tissues and lower GLYAT expression was correlated with histological grade, tumor TNM stage, Ki-67 status, and poorer survival in BC patients. Furthermore, lower GLYAT expression seemed as an independent risk factor related to poor prognosis in BC patients based on Cox regression analyses.

Conclusion: Our findings demonstrate that downregulation of GLYAT expression in human breast cancer is correlated with EMT the PI3K/AKT/Snail pathway and is also associated with histological grade, tumor TNM stage, Ki-67 status, and poor survival in breast cancer patients.
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http://dx.doi.org/10.3389/fonc.2021.641399DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8100313PMC
April 2021

The hemostatic mechanism of "Treated the Spleen" therapy on immune thrombocytopenia based on the characteristics of vasoactive factors.

Ann Palliat Med 2021 Apr;10(4):4612-4622

Department of Hematology, Dongfang Hospital Affiliated to Beijing University of Chinese Medicine, Beijing, China.

Background: To explore the effect mechanism of "treat the spleen" therapy on immune thrombocytopenia (ITP) based on the characteristics of vasoactive factors.

Methods: The ITP mice model was established by passive immunomodeling. 120 successfully modeled BALB/c mice were randomly divided into 6 groups: normal group, model group, prednisone group, Guipi Decoction group, Jianpi Yiqi group, and Jianpi Shexue group. These mice were treated with medicine for 16 days. After treatment, the platelet (PLT) counts and the degree of bleeding were evaluated, and the serum ET-1, NO, NOS3, TXA2, PGI2, vWF, VCAM-1, and TM contents of the model mice were detected by enzyme-linked immunosorbent assay (ELISA).

Results: The PLT counts in peripheral blood of mice in each experimental group significantly decreased compared with that in the normal group (P<0.01). On the 8th day after commencing administration, compared with the model group, PLT counts of mice in each experimental group significantly increased (P<0.01). Before administration, all groups had different bleeding tendencies except for the normal group. On the 6th and 8th day of drug intervention, compared with the model group, the bleeding grade of treated groups was significantly decreased (P<0.01). The values of ET-1, vWF, and VCAM-1 in each experimental group significantly decreased compared with that in the normal group, while the TXA2 values were up-regulated compared with that in the normal and model groups (P<0.01). The values of NO and TM in each experimental group significantly decreased compared with that in the normal group (P<0.05). In addition to the Guipi Decoction group, the NOS3 values in each group were significantly decreased compared with that in the normal group (P<0.05). The PGI2 values of the "treat the spleen" groups were significantly decreased compared to the normal group (P<0.01).

Conclusions: In addition to increasing the PLT counts in peripheral blood of ITP model mice to achieve a hemostatic effect, the "treat the spleen" recipes up-regulated the levels of TXA2 and VCAM-1, while down-regulating the levels of PGI2 and TM. Therefore, balancing the procoagulant and anticoagulant factors might be one of the effective mechanisms of hemostasis.
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http://dx.doi.org/10.21037/apm-21-765DOI Listing
April 2021

Delayed KCNQ1/KCNE1 assembly on the cell surface helps I fulfil its function as a repolarization reserve in the heart.

J Physiol 2021 May 7. Epub 2021 May 7.

Department of Physiology & Biophysics, Virginia Commonwealth University, Richmond, VA, USA.

Key Points: In adult ventricular myocytes, the slow delayed rectifier (I ) channels are distributed on the surface sarcolemma, not t-tubules. In adult ventricular myocytes, KCNQ1 and KCNE1 have distinct cell surface and cytoplasmic pools. KCNQ1 and KCNE1 traffic from the endoplasmic reticulum to the plasma membrane by separate routes, and assemble into I channels on the cell surface. Liquid chromatography/tandem mass spectrometry applied to affinity-purified KCNQ1 and KCNE1 interacting proteins reveals novel interactors involved in protein trafficking and assembly. Microtubule plus-end binding protein 1 (EB1) binds KCNQ1 preferentially in its dimer form, and promotes KCNQ1 to reach the cell surface. An LQT1-associated mutation, Y111C, reduces KCNQ1 binding to EB1 dimer.

Abstract: Slow delayed rectifier (I ) channels consist of KCNQ1 and KCNE1. I functions as a 'repolarization reserve' in the heart by providing extra current for ventricular action potential shortening during β-adrenergic stimulation. There has been much debate about how KCNQ1 and KCNE1 traffic in cells, where they associate to form I channels, and the distribution pattern of I channels relative to β-adrenergic signalling complex. We used experimental strategies not previously applied to KCNQ1, KCNE1 or I , to provide new insights into these issues. 'Retention-using-selected-hook' experiments showed that newly translated KCNE1 constitutively trafficked through the conventional secretory path to the cell surface. KCNQ1 largely stayed in the endoplasmic reticulum, although dynamic KCNQ1 vesicles were observed in the submembrane region. Disulphide-bonded KCNQ1/KCNE1 constructs reported preferential association after they had reached cell surface. An in situ proximity ligation assay detected I channels in surface sarcolemma but not t-tubules of ventricular myocytes, similar to the reported location of adenylate cyclase 9/yotiao. Fluorescent protein-tagged KCNQ1 and KCNE1, in conjunction with antibodies targeting their extracellular epitopes, detected distinct cell surface and cytoplasmic pools of both proteins in myocytes. We conclude that, in cardiomyocytes, KCNQ1 and KCNE1 traffic by different routes to surface sarcolemma where they assemble into I channels. This mode of delayed channel assembly helps I fulfil its function of repolarization reserve. Proteomic experiments revealed a novel KCNQ1 interactor, microtubule plus-end binding protein 1 (EB1). EB1 dimer (active form) bound KCNQ1 and increased its surface level. An LQT1 mutation, Y111C, reduced KCNQ1 binding to EB1 dimer.
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http://dx.doi.org/10.1113/JP281773DOI Listing
May 2021

The Incremental Prognostic Value of the Clinical Residual SYNTAX Score for Patients With Chronic Renal Insufficiency Undergoing Percutaneous Coronary Intervention.

Front Cardiovasc Med 2021 15;8:647720. Epub 2021 Apr 15.

The Second Medical Center & National Clinical Research Center for Geriatric Diseases, Chinese PLA General Hospital, Beijing, China.

The residual SYNTAX score (RSS) is considered a powerful prognostic indicator for determining a reasonable revascularization strategy in patients undergoing percutaneous coronary intervention (PCI), but the absence of clinical parameters is one of the limitations of RSS, especially in the chronic renal insufficiency (CRI) comorbidity setting. The present work aimed to investigate the incremental prognostic value of clinical residual SYNTAX score (CRSS) compared with RSS in CRI cases after PCI. Totally 2,468 consecutive CRI cases who underwent PCI from January 2014 to September 2017 were included in this retrospective analysis. CRSS was obtained by multiplying RSS by the modified ACEF score. Individuals with CRSS >0 were considered to have incomplete revascularization and stratified by CRSS tertiles, the remaining cases constituted the complete revascularization (CR) group. The outcomes between these groups were compared. At a median follow-up of 3 years, compared with CR group, individuals with CRSS >12 showed elevated rates of all clinical outcomes, and those with CRSS ≤ 12 showed similar all-cause and cardiac mortality rates. In multivariable analysis, CRSS was a powerful independent predictive factor of all clinical outcomes. The net reclassification improvement levels of CRSS over RSS for all-cause and cardiac mortality rates were 10.3% ( = 0.007) and 16.4% ( < 0.001), respectively. Compared with RSS, CRSS markedly ameliorated all-cause and cardiac mortality risk stratification. Compared with RSS, CRSS has incremental predictability for long-term all-cause and cardiac mortality in CRI cases following PCI.
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http://dx.doi.org/10.3389/fcvm.2021.647720DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8082103PMC
April 2021

Emerging Applications of 3D Printing in Biomanufacturing.

Trends Biotechnol 2021 Apr 27. Epub 2021 Apr 27.

College of Biotechnology and Pharmaceutical Engineering, State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Tech University, Nanjing 211816, PR China; Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University, Nanjing 211816, PR China.

The combination of 3D printing and synthetic biology is a sustainable strategy to fabricate biological objects and systems that behave in a preprogrammed manner. Many microorganisms have been genetically engineered as cell factories for the biosynthesis of chemicals using fermentation, and 3D printing of living materials using these cells could lead to a new paradigm for biomanufacturing.
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http://dx.doi.org/10.1016/j.tibtech.2021.04.005DOI Listing
April 2021

Influence of uric acid on the correlation between waist circumference and triglyceride glucose index: an analysis from CHARLS.

Lipids Health Dis 2021 Apr 30;20(1):42. Epub 2021 Apr 30.

National Office for Maternal and Child Health Surveillance of China, Department of Obstetrics, West China Second University Hospital, Sichuan University, Chengdu, 610041, P. R. China.

Background: Waist circumference (WC) and uric acid (UA) are significantly related. Still, their temporal sequence and how the sequence works on future risk of triglyceride glucose (TyG) are unknown, especially in the Chinese population.

Methods: Cross-lagged panel model was used to analyze the reciprocal, longitudinal relationships among a set of interrelated variables. The mediation model was constructed to test the effect of the relationship between WC and UA on TyG.

Results: A total of 5727 subjects were enrolled in our study population, of which 53.5% were women, and the mean age was 59.0 (standard deviation, 8.62) years. After adjusting for traditional confounding factors, the results showed that a higher level of baseline WC was significantly associated with a higher level of follow-up UA (β = 0.003, P = 0.031) and follow-up TyG (β = 0.003, P < 0.001);. Simultaneously, there was no statistical association between the level of baseline UA and the level of follow-up WC (β = - 0.009, P = 0.951). The mediation effects of UA on WC-TyG were estimated to be 18.1% in adults, and 36.2% in women.

Conclusions: The current study demonstrated that higher baseline level of WC probably preceded UA' level in general population. In addition, UA mediated the relationship of WC to TyG, especially in females. And the possible mechanism would require further clarification.
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http://dx.doi.org/10.1186/s12944-021-01474-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8086271PMC
April 2021

Corrigendum: Decreased DUSP26 Expression Promotes Malignant Behavior in Glioblastoma Cells Deregulation of MAPK and Akt Signaling Pathway.

Front Oncol 2021 12;11:676647. Epub 2021 Apr 12.

Department of Neurosurgery, Shengjing Hospital of China Medical University, Shenyang, China.

[This corrects the article DOI: 10.3389/fonc.2021.622826.].
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http://dx.doi.org/10.3389/fonc.2021.676647DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8075805PMC
April 2021