Publications by authors named "Min Jiang"

1,290 Publications

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Solamargine Alleviated UVB-Induced Inflammation and Melanogenesis in Human Keratinocytes and Melanocytes the p38 MAPK Signaling Pathway, a Promising Agent for Post-inflammatory Hyperpigmentation.

Front Med (Lausanne) 2022 13;9:812653. Epub 2022 Jun 13.

Department of Dermatology, Huashan Hospital, Fudan University, Shanghai, China.

Post-inflammatory hyperpigmentation (PIH) is a common acquired pigmentary disorder occurring after skin inflammation or injury. Ultraviolet B irradiation could exaggerate PIH clinically due to its effect on promoting cutaneous inflammation and melanogenesis in keratinocytes and melanocytes, respectively. Solamargine (SM), a steroidal alkaloid glycoside extracted from , significantly inhibits Ultraviolet B (UVB)-induced pro-inflammatory cytokines IL-1α, IL-1β, IL-8, and IFN-γ, as well as paracrine melanogenic factors ET-1, α-MSH, and bFGF in human keratinocytes. Additionally, SM significantly attenuated UVB-induced melanin synthesis in human epidermal melanocytes through down-regulation of tyrosinase activity and expression of MITF, TRP-1, TRP-2, and tyrosinase. SM exerted an anti-inflammatory effect in UVB-irradiated keratinocytes through the p38 MAPK/Nrf2/HO-1 signaling pathway. With its anti-inflammatory and whitening effect, SM may improve PIH through paracrine regulations of keratinocytes and direct action on melanocytes, making it a promising agent for PIH.
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http://dx.doi.org/10.3389/fmed.2022.812653DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9234656PMC
June 2022

SECISBP2L-mediated selenoprotein synthesis is essential for autonomous regulation of oligodendrocyte differentiation.

J Neurosci 2022 Jun 27. Epub 2022 Jun 27.

Institute of Life Sciences, Key Laboratory of Organ Development and Regeneration of Zhejiang Province, College of Life Sciences, Hangzhou Normal University, 2318 Yuhangtang Road, Hangzhou, Zhejiang, 311121, PR China

Thyroid hormone controls the timely differentiation of oligodendrocytes (OLs), and its deficiency can delay myelin development and cause mental retardation. Previous studies showed that the active thyroid hormone T3 is converted from its prohormone T4 by the selenoprotein DIO2, whose mRNA is primarily expressed in astrocytes in the central nervous system. In the present study, we discovered that SECISBP2L is highly expressed in differentiating OLs and is required for DIO2 translation. Conditional knockout of Secisbp2l in OL lineage resulted in a decreased level of DIO2 and T3, accompanied by impaired OL differentiation, hypomyelination and motor deficits in both sexes of mice. Moreover, the defective differentiation of OLs in Secisbp2l mutants can be alleviated by T3 or its analog, but not the prohormone T4. The present study has provided strong evidence for the autonomous regulation of OL differentiation by its intrinsic T3 production mediated by the novel SECISBP2L-DIO2-T3 pathway during myelin development.Secisbp2l is specifically expressed in differentiating oligodendrocytes and is essential for selenoprotein translation in oligodendrocytes. Secisbp2l regulates Dio2 translation for active thyroid hormone T3 production in the central nervous system. Autonomous regulation of oligodendrocytes differentiation via SECISBP2L-DIO2-T3 pathway.
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http://dx.doi.org/10.1523/JNEUROSCI.2141-21.2022DOI Listing
June 2022

Floquet Spin Amplification.

Phys Rev Lett 2022 Jun;128(23):233201

Helmholtz-Institut, GSI Helmholtzzentrum für Schwerionenforschung, Mainz 55128, Germany.

Detection of weak electromagnetic waves and hypothetical particles aided by quantum amplification is important for fundamental physics and applications. However, demonstrations of quantum amplification are still limited; in particular, the physics of quantum amplification is not fully explored in periodically driven (Floquet) systems, which are generally defined by time-periodic Hamiltonians and enable observation of many exotic quantum phenomena such as time crystals. Here we investigate the magnetic-field signal amplification by periodically driven ^{129}Xe spins and observe signal amplification at frequencies of transitions between Floquet spin states. This "Floquet amplification" allows us to simultaneously enhance and measure multiple magnetic fields with at least one order of magnitude improvement, offering the capability of femtotesla-level measurements. Our findings extend the physics of quantum amplification to Floquet spin systems and can be generalized to a wide variety of existing amplifiers, enabling a previously unexplored class of "Floquet spin amplifiers".
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http://dx.doi.org/10.1103/PhysRevLett.128.233201DOI Listing
June 2022

MV-H-RKM: A Multiple View-based Hypergraph Regularized Restricted Kernel Machine for Predicting DNA-binding Proteins.

IEEE/ACM Trans Comput Biol Bioinform 2022 Jun 22;PP. Epub 2022 Jun 22.

DNA-binding proteins (DBPs) have a significant impact on many life activities, so identification of DBPs is a crucial issue. And it is greatly helpful to understand the mechanism of protein-DNA interactions. In traditional experimental methods, it is significant time-consuming and labor-consuming to identify DBPs. In recent years, many researchers have proposed lots of different DBP identification methods based on machine learning algorithm to overcome shortcomings mentioned above. However, most existing methods cannot get satisfactory results. In this paper, we focus on developing a new predictor of DBPs, called Multi-View Hypergraph Restricted Kernel Machines (MV-H-RKM). In this method, we extract five features from the three views of the proteins. To fuse these features, we couple them by means of the shared hidden vector. Besides, we employ the hypergraph regularization to enforce the structure consistency between original features and the hidden vector. Experimental results show that the accuracy of MV-H-RKM is 84.09% and 85.48% on PDB1075 and PDB186 data set respectively, and demonstrate that our proposed method performs better than other state-of-the-art approaches. The code is publicly available at https://github.com/ShixuanGG/MV-H-RKM.
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http://dx.doi.org/10.1109/TCBB.2022.3183191DOI Listing
June 2022

Rapidly regenerated CNC/TiO/MnO porous microspheres for high-efficient dye removal.

Carbohydr Polym 2022 Sep 24;292:119644. Epub 2022 May 24.

Key Laboratory of Rubber-Plastics, Ministry of Education/Shandong Provincial Key Laboratory of Rubber-plastics, Qingdao University of Science & Technology, Qingdao 266042, China. Electronic address:

The cellulose nanocrystal (CNC)/transition metal oxide hybrid porous microspheres usually have high adsorption performance and degradability, and show promising applications in the field of wastewater treatment. However, single metal oxides are limited by the forbidden band width and electron-hole complexation, and often fail to achieve good effluent treatment. Herein, a CNC/manganese dioxide/titanium dioxide porous microspheres are prepared by the combination of bubble template and ionic crosslinking between sodium alginate and Ca ions as a matrix. Based on the synergistic effect of two kinds of transition metal oxide, the degradation efficiency of CNC/MnO/TiO porous microspheres for methylene blue is as high as 97.0%, showing easy recovery process and rapid regeneration of this material. Besides, its maximum removal of dyes reaches up to 310.2 mg/g, which is much higher than most CNC based adsorption materials. The concept of concerted catalysis can to explain the origin for excellent performance of as-prepared hybrid microspheres.
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http://dx.doi.org/10.1016/j.carbpol.2022.119644DOI Listing
September 2022

SERPINA5 may promote the development of preeclampsia by disruption of the uPA/uPAR pathway.

Transl Res 2022 Jun 15. Epub 2022 Jun 15.

Department of Laboratory, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, 510623, China. Electronic address:

Preeclampsia (PE) is the leading cause of maternal and fetal morbidity or mortality but lacks reliable methods for early diagnosis. In a previous study, serum SERPINA5 levels were higher in women with PE before the clinical manifestation of the disease. This study aimed to evaluate the efficacy of SERPINA5 in predicting PE and investigate its role in trophoblast cell biology. A multicenter, two-stage observational case-control study was performed to develop and validate an early predictive PE model based on SERPINA5, maternal characteristics, and inflammatory factors. To further understand the relationship between SERPINA5 and PE, SERPINA5 was overexpressed or knocked down in extravillous trophoblast cells (EVT) and a pregnant rat model. After development and initial validation, a model that combined SERPINA5 and inflammatory factors had a high predictive ability for PE before 20 weeks gestation with an AUC of 0.90 (95% CI 0.83-0.96). It also demonstrated that SERPINA5 inhibited primary EVT cell invasion by disrupting the urokinase-type plasminogen activator/urokinase-type plasminogen activator receptor (uPA/uPAR) pathway, in turn, is involved in the development of PE. In vivo experiments also proved that overexpression of SERPINA5 induced a PE-like syndrome (hypertension and proteinuria) in pregnant rats. Therefore, serum SERPINA5 is a promising early biomarker of PE, suggesting that it may be involved in placental development through its action on the uPA/uPAR system prior to the clinical manifestation of PE.
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http://dx.doi.org/10.1016/j.trsl.2022.06.011DOI Listing
June 2022

Evaluating an external quality assurance program for semen analysis in China during 2009-2020.

Andrology 2022 Jun 14. Epub 2022 Jun 14.

NHC Key Laboratory of Male Reproduction and Genetics, Guangdong Provincial Reproductive Science Institute (Guangdong Provincial Fertility Hospital), Guangzhou, China.

Introduction: Semen analysis (SA) plays a key role in guiding treatments of male reproductive diseases and infertility due to male factors; however, it remains challenging to conduct an accurate SA due to lack of standardization, highly subjective assessments, and problems with automated procedures. Therefore, quality assurance (QA) and teaching courses are essential for making the laboratory results more consistent.

Materials And Methods: The external quality assurance (EQA) scheme was organized by national human sperm bank technology training bases in Guangdong province in China between 2009 and 2020. Until 2020, 124 laboratories from China participated in the EQA program. The EQA scheme per year has been organized involving two semen aliquots for sperm concentration, two video recordings for motility, and two smears for sperm morphology. All samples used in the EQA scheme were obtained from different healthy donors or patients.

Results: We estimated that the median coefficient of variation (CV) of sperm concentration, ignoring the method used, was 26.6%. Using a 100 µm deep counting chamber led to a decreasing CV of 13.6%. For sperm motility, the median CV of nonprogressive motility was high (50.8%), but the CV of progressive motility (13.2%), immotile sperm (14.3%), and total motility (11.8%) were acceptable. The morphology assessment revealed large variability (44.4%) irrespective of the classification criteria.

Discussion: The reduction of interlaboratory variability is still a challenge during SA in China. Therefore, it is critical to increase awareness of joining EQA schemes and establish standardized training centers to follow WHO-recommended procedures toward Chinese standards.
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http://dx.doi.org/10.1111/andr.13205DOI Listing
June 2022

Biodegradation of polyether-polyurethane foam in yellow mealworms (Tenebrio molitor) and effects on the gut microbiome.

Chemosphere 2022 Oct 10;304:135263. Epub 2022 Jun 10.

State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, 211800, PR China. Electronic address:

Polyurethane (PU) is one of the mass-produced recalcitrant plastics with a high environmental resistance but extremely low biodegradability. Therefore, improperly disposed PU waste adds significantly to plastic pollution, which must be addressed immediately. In recent years, there has been an increasing number of reports on plastic biodegradation in insect larvae, especially those that can feed on polyethylene and polystyrene. This study revealed that yellow mealworm (Tenebrio molitor) larvae can chew and ingest polyether-PU foams efficiently, resulting in a significant mass loss of nearly 67% after 35 days at a similar survival rate compared to when fed on bran. However, polyether-PU fragments were found in the frass of T. molitor, indicating that polyether-PU biodegradation and bioconversion in intestinal tracts were not complete. The scission of ether and urethane bonds in the polyether-PU can be evidenced by comparing polymer fragments recovered from frass with the pristine ones using Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy. Gel permeation chromatography suggested the release of low-molecular-weight oligomers as a result of the biodegradation, which also resulted in poor thermal stability of the polyether-PU foam as determined by thermogravimetric analysis. High-throughput sequencing of the gut microbiome revealed significant changes in the microbial community populations due to the polyether-PU diet, for example, an increase in the families Enterobacteriaceae and Streptococcaceae, suggesting that these microorganisms may contribute to the polyether-PU biodegradation.
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http://dx.doi.org/10.1016/j.chemosphere.2022.135263DOI Listing
October 2022

Current status, challenges and prospects for lignin valorization by using Rhodococcus sp.

Biotechnol Adv 2022 Jun 9;60:108004. Epub 2022 Jun 9.

State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, PR China; Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University, Nanjing 211816, PR China. Electronic address:

Lignin represents the most abundant renewable aromatics in nature, which has complicated and heterogeneous structure. The rapid development of biotransformation technology has brought new opportunities to achieve the complete lignin valorization. Especially, Rhodococcus sp. possesses excellent capabilities to metabolize aromatic hydrocarbons degraded from lignin. Furthermore, it can convert these toxic compounds into high value added bioproducts, such as microbial lipids, polyhydroxyalkanoate and carotenoid et al. Accordingly, this review will discuss the potentials of Rhodococcus sp. as a cell factory for lignin biotransformation, including phenol tolerance, lignin depolymerization and lignin-derived aromatic hydrocarbon metabolism. The detailed metabolic mechanism for lignin biotransformation and bioproducts spectrum of Rhodococcus sp. will be comprehensively discussed. The available molecular tools for the conversion of lignin by Rhodococcus sp. will be reviewed, and the possible direction for lignin biotransformation in the future will also be proposed.
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http://dx.doi.org/10.1016/j.biotechadv.2022.108004DOI Listing
June 2022

Prognostic value of dynamic cardiac biomarkers in patients with acquired refractory thrombocytopenic purpura: A retrospective study in Chinese population.

J Clin Lab Anal 2022 Jun 11:e24547. Epub 2022 Jun 11.

Hematological Intensive Care Unit,Department of Hematology, The First Affiliated Hospital of Soochow University, Suzhou, China.

Introduction: Thrombotic thrombocytopenic purpura (TTP) is becoming a curable disease with the introduction of therapeutic plasma exchange (TPE). However, cardiovascular complications remain essential causes of mortality in patients with refractory TTP, while the association of cardiac biomarkers with the prognosis of TTP warrants further investigation.

Methods: Patients admitted to the First Affiliated Hospital of Soochow University for refractory TTP from 2013 through 2020 were included in this retrospective study. Clinical characteristics were collected from electronic health records. Biomarker levels on admission and post TPE were recorded. Logistic regression was adopted to identify risk factors for mortality.

Results: A total of 78 patients with refractory TTP were included in this study. Twenty-one patients died during hospitalization, with a mortality rate of 26.9%. High-sensitivity cardiac troponin T (hs-cTnT), N-terminal probrain natriuretic peptide (NT-proBNP), and aspartate aminotransferase (AST) and alanine aminotransferase (ALT) ratios (AAR) were increased in deceased patients compared with the survival group. Multivariate analysis showed that AAR after TPE was associated with overall mortality (OR: 4.45, 95% CI 1.09-18.19). The areas under the receiver operator characteristic curve (AUC) of AAR, hs-cTnT, and NT-proBNP for the association with mortality were 0.814, 0.840, and 0.829, respectively.

Conclusion: Higher post-TPE cardiac biomarker levels are associated with increased in-hospital mortality in patients with refractory TTP.
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http://dx.doi.org/10.1002/jcla.24547DOI Listing
June 2022

Dissemination of virulence and resistance genes among Klebsiella pneumoniae via outer membrane vesicle: An important plasmid transfer mechanism to promote the emergence of carbapenem-resistant hypervirulent Klebsiella pneumoniae.

Transbound Emerg Dis 2022 Jun 9. Epub 2022 Jun 9.

MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.

Klebsiella pneumoniae is well-known opportunistic enterobacteria involved in complex clinical infections in humans and animals. The domestic animals might be a source of the multidrug-resistant virulent K. pneumoniae to humans. K. pneumoniae infections in domestic animals are considered as an emergent global concern. The horizontal gene transfer plays essential roles in bacterial genome evolution by spread of virulence and resistance determinants. However, the virulence genes can be transferred horizontally via K. pneumoniae-derived outer membrane vesicles (OMVs) remains to be unreported. In this study, we performed complete genome sequencing of two K. pneumoniae HvK2115 and CRK3022 with hypervirulent or carbapenem-resistant traits. OMVs from K. pneumoniae HvK2115 and CRK3022 were purified and observed. The carriage of virulence or resistance genes in K. pneumoniae OMVs was identified. The influence of OMVs on the horizontal transfer of virulence-related or drug-resistant plasmids among K. pneumoniae strains was evaluated thoroughly. The plasmid transfer to recipient bacteria through OMVs was identified by polymerase chain reaction, pulsed field gel electrophoresis and Southern blot. This study revealed that OMVs could mediate the intraspecific and interspecific horizontal transfer of the virulence plasmid phvK2115. OMVs could simultaneously transfer two resistance plasmids into K. pneumoniae and Escherichia coli recipient strains. OMVs-mediated horizontal transfer of virulence plasmid phvK2115 could significantly enhance the pathogenicity of human carbapenem-resistant K. pneumoniae CRK3022. The CRK3022 acquired the virulence plasmid phvK2115 could become a CR-hvKp strain. It was critically important that OMVs-mediated horizontal transfer of phvK2115 lead to the coexistence of virulence and carbapenem-resistance genes in K. pneumoniae, resulting in the emerging of carbapenem-resistant hypervirulent K. pneumoniae.
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http://dx.doi.org/10.1111/tbed.14615DOI Listing
June 2022

CaMKII, that binds with ligustilide, as a potential drug target of Suxiao jiuxin pill, a traditional Chinese medicine to dilate thoracic aorta.

Clin Transl Med 2022 Jun;12(6):e907

State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin, China.

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http://dx.doi.org/10.1002/ctm2.907DOI Listing
June 2022

Exploring the Q-markers of Angelica sinensis (Oliv.) Diels of anti-platelet aggregation activity based on spectrum-effect relationships.

Biomed Chromatogr 2022 Jun 8:e5422. Epub 2022 Jun 8.

School of Pharmacy, Lanzhou University, Lanzhou, China.

The radix of Angelica sinensis (Oliv.) Diels (RAS) is widely used in medicinal and dietary applications in China, and has the function for replenishing and invigorating the blood, stopping pain and moistening the intestines. In this study, RAS from the main geoherb regions showed better efficacy in inhibiting Adenosine diphosphate- or arachidonic acid-induced platelet aggregation than those from non-geoherb regions. In addition, the HPLC fingerprints of 30 batches of RAS, as part of the comprehensive evaluation of RAS, were established and used for spectral efficiency to screen the quality markers for anti-platelet aggregation activities. Five compounds in RAS-senkyunolide I, uridine, guanine, ferulic acid and adenosine-were demonstrated to contribute significantly to the anti-platelet aggregation activity. These bioactive compounds, especially senkyunolide I and ferulic acid with stronger activities, could be used as quality markers of RAS for quality control of RAS.
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http://dx.doi.org/10.1002/bmc.5422DOI Listing
June 2022

Transcription-Associated Fluorescence-Activated Droplet Sorting for Di-rhamnolipid Hyperproducers.

ACS Synth Biol 2022 Jun 31;11(6):1992-2000. Epub 2022 May 31.

College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, China.

Rhamnolipids (RLs) are biosurfactants with great economic significance that have been used extensively in multiple industries. is a promising microorganism for sustainable RL production. However, current CTAB-MB based screening of RL-producing strains is time-consuming, labor-intensive, and unable to distinguish mono- and di-RL. In this study, we developed a novel transcription-associated fluorescence-activated droplet sorting (FADS) method to specifically target the di-RL hyperproducers. We first investigated critical factors associated with this method, including the specificity and sensitivity for discriminating di-RL overproducers from other communities. Validation of genotype-phenotype linkage between the GFP intensity, transcription, and di-RL production showed that transcription is closely correlated with di-RL production, and the GFP intensity is responsive to transcription, respectively. Using this platform, we screened out ten higher di-RL producing microorganisms, which produced 54-208% more di-RL than the model PAO1. In summary, the droplet-based microfluidic platform not only facilitates a more specific, reliable, and rapid screening of colonies with desired phenotypes, but also shows that intracellular transcription-associated GFP intensity can be used to measure the yield of di-RL between populations of droplets containing different environmental colonies. This method also can be integrated with transposon mutation libraries to target mutants.
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http://dx.doi.org/10.1021/acssynbio.1c00622DOI Listing
June 2022

Comparison of four neonatal transport scoring methods in the prediction of mortality risk in full-term, out-born infants: a single-center retrospective cohort study.

Eur J Pediatr 2022 May 26. Epub 2022 May 26.

Neonatal Center, Beijing Children's Hospital, Capital Medical University, Beijing, China.

Neonatal transport scoring systems can assess severity before and after transport, improve transport efficiency, and predict the occurrence of critical illness. The aim of this study was to compare four neonatal transport scoring methods to predict mortality risk and clinical utility within the first week after transportation. This was a single-center retrospective cohort study. All patients were full-term, out-born neonates. Each patient was assessed by the Transport Risk Index of Physiologic Stability (TRIPS), Mortality Index for Neonatal Transportation (MINT), Transport-Related Mortality Score (TREMS), and Neonatal Critical Illness Score (NCIS) scoring methods. Receiver operating characteristic (ROC) curves and decision curve analysis (DCA) for each method were compared for their utility in predicting mortality risk within the 1st week after admission. In total, 368 full-term infants were included (368/770, 47.8% of all transported infants). Within the 1st week after admission, five infants (1.36%, 5/368) died while receiving advanced life support and full treatment, and 24 infants (6.52%, 24/368) died soon after they were discharged against medical advice. The areas under the curve (AUCs) for the MINT, TRIPS, TREMS, and NCIS for the prediction of mortality were 0.822, 0.827, 0.643, and 0.731, respectively (all p < 0.05). However, the clinical net benefits for the MINT and TRIPS were far superior than those for the NCIS and TREMS.

Conclusion: It was concluded that the TRIPS and MINT might be more suitable for the prediction of mortality in full-term, out-born neonates in the neonatal intensive care unit (NICU) within the 1st week after transportation.

What Is Known: • Neonatal transport scores can assess not only the mortality risk during transportation but also the mortality risk of critically ill newborns after admission to the NICU. • The effectiveness of neonatal transport scores in predicting mortality risk is different.

What Is New: • Our data indicate that the diagnostic efficacy of the MINT, TRIPS, and NCIS in the prediction of full-term infant mortality was high. • The TRIPS and MINT scores had better clinical utility and could be used to predict mortality within the 1st week after transportation in full-term out-born neonates.
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http://dx.doi.org/10.1007/s00431-022-04506-8DOI Listing
May 2022

Dual-modality Imaging of Angiogenesis in Unstable Atherosclerotic Plaques with VEGFR2-Targeted Upconversion Nanoprobes in vivo.

Mol Imaging Biol 2022 May 23. Epub 2022 May 23.

Medical School of Chinese PLA, Department of Cardiology in Second Medical Center &, National Research Center for Geriatric Diseases, Chinese PLA General Hospital, Beijing, 100853, China.

Aim: Angiogenesis plays a major role in atherosclerotic plaque development and instability. Our study aims to develop a novel optical and magnetic resonance (MR) dual-modality molecular imaging probe to early detect unstable plaques in vivo by targeting biomarkers of angiogenesis in murine models of atherosclerosis (AS).

Methods: Immunofluorescence and western blot were used to detect the expression of Vascular Endothelial Growth Factor Receptor 2 (VEGFR2) in activated Human Umbilical Vein Endothelial Cells (HUVECs). After synthesis and identification of novel short peptide VRBP1-targeted VEGFR2, HUVECs were co-cultured with FITC-VRBP1 to test specific affinity of VRBP1. Then VRBP1-UCNPstargeting VEGFR2 were constructed by conjugating VRBP1 to the surface of NaGdF:Yb,[email protected] nanoparticles. The characterization of the nanoparticles was performed by transmission electron microscopy (TEM), distribution of size, hydrodynamic size, zeta potential, absorption spectra, emission spectra, imaging intensity of different concentrations, binding affinity and cytotoxicity of nanoprobes in vitro. The upconversion luminescence (UCL) and MR imaging were performed to identify unstable atherosclerotic plaque in ApoE mice in vivo and ex vivo. Morphological staining was used to verify AS model and angiogenesis, and Inductively Coupled Plasma-Atomic Emission Spectrometry (ICP-AES) was used to confirm accumulation of the nanoparticles after imaging.

Results: After induced by hypoxia and ox-LDL, the expression of VEGFR2 in activated HUVECs was enhanced. FITC-VRBP1 can specifically bind to the HUVECs. Characterization of the nanoparticles showed that particles size is uniform with a stable structure, specific optical and MR signal, good binding affinity to VEGFR2 and low cytotoxicity. In vivo and ex vivo UCL imaging and quantitative analysis revealed that distinctive optical signal was observed in the regions of left carotid common arteries (LCCAs) of AS group after injection of VRBP1-UCNPs. Higher signal intensity on T1-weighted MR imaging appeared in the LCCA wall of AS group after injection. The results of morphological staining demonstrated angiogenesis in the atherosclerotic plaques, Gd ions in LCCAs, aortic arch and renal arteries bifurcations detected by ICP-AES confirmed accumulation of the nanoparticles in plaque.

Conclusions: We successfully design and synthesize a novel UCNPs using peptide VRBP1 targeting to VEGFR2. In vivo imaging demonstrates that VRBP1-UCNPs can be used to perform optical/MR dual-modality imaging targeting angiogenesis in plaques, which is a promising technique to early detect unstable atherosclerosis.
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http://dx.doi.org/10.1007/s11307-022-01721-5DOI Listing
May 2022

Rapid Multilateral and Integrated Public Health Response to a Cross-City Outbreak of Enteritidis Infections Combining Analytical, Molecular, and Genomic Epidemiological Analysis.

Front Microbiol 2022 4;13:772489. Epub 2022 May 4.

Shenzhen Major Infectious Disease Control Key Laboratory, Shenzhen Center for Disease Control and Prevention, Shenzhen, China.

On September 21, 2019, the Shenzhen and Dongguan Centers for Disease Control and Prevention received notification of a large cluster of suspected gastroenteritis involving primarily children who sought medical care at hospitals throughout two adjacent cities in China, Shenzhen, and Dongguan. A joint outbreak response was promptly initiated across jurisdictions in a concerted effort between clinical microbiologists, epidemiologists, and public health scientists. Concurrently, multiplex PCRs were used for rapid laboratory diagnosis of suspected cases; epidemiological investigations were conducted to identify the outbreak source, complemented by near real-time multicenter whole-genome analyses completed within 34 h. Epidemiological evidence indicated that all patients had consumed egg sandwiches served on September 20 as snacks to children and staff at a nursery in Dongguan, located near Shenzhen. Enteritidis was isolated from case-patients, food handlers, kitchenware, and sandwiches with kitchen-made mayonnaise. Whole-genome single-nucleotide polymorphism (SNP)-based phylogenetic analysis demonstrated a well-supported cluster with pairwise distances of ≤1 SNP between genomes for outbreak-associated isolates, providing the definitive link between all samples. In comparison with historical isolates from the same geographical region, the minimum pairwise distance was >14 SNPs, suggesting a non-local outbreak source. Genomic source tracing revealed the possible transmission dynamics of a . Enteritidis clone throughout a multi-provincial egg distribution network. The efficiency and scale with which multidisciplinary and integrated approaches were coordinated in this foodborne disease outbreak response was unprecedented in China, leading to the timely intervention of a large cross-jurisdiction outbreak.
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http://dx.doi.org/10.3389/fmicb.2022.772489DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9117964PMC
May 2022

What can AI-TENG do for Low Abundance Biosensing?

Front Bioeng Biotechnol 2022 5;10:899858. Epub 2022 May 5.

Chongqing Key Laboratory of Nonlinear Circuits and Intelligent Information Processing, College of Electronic and Information Engineering, Southwest University, Chongqing, China.

Biosensing technology helps prevent, diagnose, and treat diseases and has attracted more and more researchers in recent years. Artificial intelligence-based triboelectric nanogenerators (AI-TENG) are promising for applications in biosensors due to their myriad of merits, including high efficiency and precision, low cost, light weight, and self-powered. This article aims to show how artificial intelligence and triboelectric nanogenerators have been combined to develop biosensors. We first focus on the working principle of triboelectric nanogenerators and the method of combining them with artificial intelligence. Secondly, we highlight the representative research work of AI-TENG in biomolecules sensing, organic compounds, and complex mixture of cells. Finally, this paper concludes with a summary and prospect on the existing challenges and possible solutions in the application of AI-TENG to the field of biosensors.
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http://dx.doi.org/10.3389/fbioe.2022.899858DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9117749PMC
May 2022

Transauricular Vagal Nerve Stimulation at 40 Hz Inhibits Hippocampal P2X7R/NLRP3/Caspase-1 Signaling and Improves Spatial Learning and Memory in 6-Month-Old APP/PS1 Mice.

Neuromodulation 2022 May 17. Epub 2022 May 17.

Clinical Medical College of Acupuncture, Moxibustion, and Rehabilitation, Guangzhou University of Chinese Medicine, Guangzhou, China. Electronic address:

Objectives: Transauricular vagal nerve stimulation (taVNS) at 40 Hz attenuates hippocampal amyloid load in 6-month-old amyloid precursor protein/presenilin 1 (APP/PS1) transgenic mice, but it is unclear whether 40-Hz taVNS can improve cognition in these mice. Moreover, the underlying mechanisms are still unclear.

Materials And Methods: 6-month-old C57BL/6 (wild type [WT]) and APP/PS1 mice were subjected to 40-Hz taVNS. Novel Object Recognition and the Morris Water Maze were used to evaluate cognition. Hippocampal amyloid-β (Aβ)1-40, Aβ1-42, pro-interleukin (IL)-1β, and pro-IL-18 were measured using enzyme-linked immunosorbent assays. Hippocampal Aβ42, purinergic 2X7 receptor (P2X7R), nucleotide-binding oligomerization domain-like receptor pyrin domain containing 3 (NLRP3), Caspase-1, IL-1β, and IL-18 expression were evaluated by western blotting. Histologic assessments including immunofluorescence, immunohistochemistry, Nissl staining, and Congo red staining were used to assess microglial phagocytosis, neuroprotective effects, and Aβ plaque load.

Results: 40-Hz taVNS improved spatial memory and learning in 6-month-old APP/PS1 mice but did not affect recognition memory. There were no effects on the cognitive behaviors of 6-month-old WT mice. taVNS at 40 Hz modulated microglia; significantly decreased levels of Aβ1-40, Aβ1-42, pro-IL-1β, and pro-IL-18; inhibited Aβ42, P2X7R, NLRP3, Caspase-1, IL-1β, and IL-18 expression; reduced Aβ deposits; and had neuroprotective effects in the hippocampus of 6-month-old APP/PS1 mice. These changes were not observed in 6-month-old WT mice.

Conclusion: Our results show that 40-Hz taVNS inhibits the hippocampal P2X7R/NLRP3/Caspase-1 signaling and improves spatial learning and memory in 6-month-old APP/PS1 mice.
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http://dx.doi.org/10.1016/j.neurom.2022.03.011DOI Listing
May 2022

Berberine as a Potential Agent for the Treatment of Colorectal Cancer.

Front Med (Lausanne) 2022 28;9:886996. Epub 2022 Apr 28.

Department of Gastroenterology, Shengjing Hospital of China Medical University, Shenyang, China.

Colorectal cancer (CRC) is one of the most commonly diagnosed and deadly malignancies worldwide. The incidence of CRC has been increasing, especially in young people. Although great advances have been made in managing CRC, the prognosis is unfavorable. Numerous studies have shown that berberine (BBR) is a safe and effective agent presenting significant antitumor effects. Nevertheless, the detailed underlying mechanism in treating CRC remains indistinct. In this review, we herein offer beneficial evidence for the utilization of BBR in the management and treatment of CRC, and describe the underlying mechanism(s). The review emphasizes several therapeutic effects of BBR and confirms that BBR could suppress CRC by modulating gene expression, the cell cycle, the inflammatory response, oxidative stress, and several signaling pathways. In addition, BBR also displays antitumor effects in CRC by regulating the gut microbiota and mucosal barrier function. This review emphasizes BBR as a potentially effective and safe drug for CRC therapy.
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http://dx.doi.org/10.3389/fmed.2022.886996DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9096113PMC
April 2022

Urolithin A protects human dermal fibroblasts from UVA-induced photoaging through NRF2 activation and mitophagy.

J Photochem Photobiol B 2022 Jul 11;232:112462. Epub 2022 May 11.

Department of Dermatology, Huashan Hospital, Fudan University, Shanghai, PR China. Electronic address:

Photoaging, caused by exposure to sunlight and especially UVA, has been identified as one of the culprits for age-related skin deterioration. Here, we initially demonstrated that urolithin A (UroA), a metabolite derived from intestine microflora, possessed sufficient photoprotective capacity and attenuated UVA-induced senescent phenotypes in human fibroblasts, such as growth inhibition, senescence-associated β-galactosidase activity, breakdown of extracellular matrix, synthesis of senescence-associated secretory phenotypes and cell cycle arrest. Furthermore, UroA lessened the accumulation of intracellular reactive oxygen species, which promoted the phosphorylation and afterwards nuclear translocation of NRF2, subsequently driving the activation of downstream antioxidative enzymes. In parallel, we proved that UroA restored mitochondrial function by induction of mitophagy, which was regulated by the SIRT3-FOXO3-PINK1-PARKIN network. Taken together, our results showed that UroA protected dermal fibroblast from UVA damage through NRF2/ARE activation and mitophagy process, thus supporting UroA as a potential therapeutic agent for photoaging.
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http://dx.doi.org/10.1016/j.jphotobiol.2022.112462DOI Listing
July 2022

Bio-effects of bio-based and fossil-based microplastics: Case study with lettuce-soil system.

Environ Pollut 2022 Aug 4;306:119395. Epub 2022 May 4.

Division of Energy Materials (DNL 22), Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China.

Bio-based plastics have been developed as alternative materials to solve the energy crisis brought by plastic production, but their impacts on soil ecosystems (e.g. plant and microorganisms) remain largely unknown. Here, we conducted study on the impacts of polyethylene 2,5-furan-dicarboxylate (PEF), a new bio-based plastic, on the plant-soil ecosystem, with comparison of fossil-based plastic polyethylene terephthalate (PET). Our investigation showed that, after 21 days exposure to microplastics (MPs) at doses of 0.5%, 1% and 2%, both PEF and PET MPs inhibited the growth of lettuce, where chlorophyll was found to be the most sensitive index. According to the comprehensive stress resistance indicators, PET MPs showed more severe phytotoxicity than PEF MPs. Although both PEF and PET MPs could inhibit soil enzyme activities, PET MPs exhibited significantly reduction on the diversity of rhizosphere soil bacterial community and changed the relative abundance of dominant species. Our study gave insights into the effects of PEF and PET MPs on the plant-soil system, where bio-based PEF MPs showed more friendly interaction with plant and soil than fossil-based PET MPs. Our results provided scientific data for risk assessment and useful information for the prospective application of bio-based plastics.
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http://dx.doi.org/10.1016/j.envpol.2022.119395DOI Listing
August 2022

Boosting hydrogen and oxygen evolution of porous CoP nanosheet arrays through electronic modulating with oxygen-anion-incorporation.

J Colloid Interface Sci 2022 Sep 20;622:239-249. Epub 2022 Apr 20.

State Key Laboratory for Powder Metallurgy, Central South University, Changsha 410083, PR China. Electronic address:

The exploration of earth-abundant single catalyst with high-efficiency bifunctional catalytic active sites for accelerated hydrogen and oxygen evolution reactions (HER and OER) is highly desirable but challenging. Herein, the synthesis of self-supported directional flake oxygen-incorporated cobalt phosphide arrays (O-CoP) with efficient bifunctional catalytic active sites was achieved by in situ oxidation followed by phosphorization of cobalt metal-organic framework nanosheet arrays (Co-MOF). By controlling the phosphating time, the P/O atomic ratio in the oxygen-incorporated cobalt phosphide could be adjusted, leading to the change of Co/Co couples, and thus affecting the electronic environment of the cobalt active site. Benefiting from the tunable electronic structure and unique array architecture, the synthesized catalysts exhibited excellent electrocatalytic water decomposition for both HER and OER. Moreover, in the HER and OER couple system (HER||OER), the optimal O-CoP-40 catalyst delivers a low overpotential of only 1.54 V to obtain the 10 mA cm and stably-running for 36 h. Theoretical calculations demonstrated that the electron-rich P-3p and O-2p orbitals could co-modulate the electronic environment of Co sites, which boosted water dissociation in the HER process and balanced the adsorption/desorption of intermediates in the OER pathway, resulting in a good overall water splitting. This research provides an effective strategy for the construction of efficient bifunctional phosphide electrocatalysts, as well as contributes to the understanding of anion incorporating to regulate the electronic structure of phosphides.
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http://dx.doi.org/10.1016/j.jcis.2022.04.013DOI Listing
September 2022

Isolated splenic metastasis of endometrial cancer 12 years after treatment: A case report and literature review.

Medicine (Baltimore) 2022 Apr 29;101(17):e29178. Epub 2022 Apr 29.

Department of Pathology, Taizhou People's Hospital Affiliated to Dalian Medical University, Taizhou, Jiangsu, China.

Rationale: The spleen is an uncommon metastatic organ for malignant solid tumors because of its special anatomy and microenvironment. Isolated splenic metastasis of endometrial cancer is an extremely rare clinical event, with only 17 cases reported in literature.

Patient Concerns: We report the case of a 58-year-old woman with abdominal distension and nausea for 7 months who had undergone surgery and chemotherapy for endometrioid adenocarcinoma 12 years previously. A space-occupying lesion in the upper pole of the spleen was observed on an abdominal ultrasound.

Diagnosis: The spleen was resected, and splenic metastasis of endometrial adenocarcinoma was histologically confirmed.

Interventions: Splenectomy was performed, and no lymph nodes or other metastases were observed. The patient received postoperative chemotherapy with 6 cycles of docetaxel and carboplatin.

Outcomes: The patient recovered well 11 months postoperatively, with no evidence of recurrence or metastatic disease.

Lesson: Since the time interval between the diagnosis of primary endometrial cancer and splenic metastasis may be very long, it may be necessary to monitor the recurrence of endometrial cancer after primary treatment.
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http://dx.doi.org/10.1097/MD.0000000000029178DOI Listing
April 2022

ING4 Promotes Stemness Enrichment of Human Renal Cell Carcinoma Cells Through Inhibiting DUSP4 Expression to Activate the p38 MAPK/type I IFN-Stimulated Gene Signaling Pathway.

Front Pharmacol 2022 14;13:845097. Epub 2022 Apr 14.

Key Laboratory of Human Genetics and Environmental Medicine, School of Public Health, Xuzhou Medical University, Xuzhou, China.

Renal cell carcinoma (RCC) recurs frequently due to high metastatic spread, resulting in a high mortality. Cancer stem cells play a critical role in initiating the tumor metastasis. Inhibitor of growth 4 (ING4) is a member of the ING family, but its impact on cancer stem cells in RCC is still unknown. In this study, we found that ING4 significantly promoted the sphere-forming size and number of RCC cells under an ultralow-attachment culture condition , tumor growth and metastasis , and the expression of some stem-like or pluripotent biomarkers CD44, MYC, OCT4, and NANOG, indicating that ING4 increased the stemness enrichment of RCC cells. Mechanistically, the ING4-activated p38 MAPK pathway possibly upregulated the expression of type I IFN-stimulated genes to promote the formation of RCC stem cells. ING4 could inhibit the expression of DUSP4 to activate p38 MAPK. In addition, selective pharmacological p38 MAPK inhibitors could significantly inhibit stemness enrichment only in ING4-overexpressed RCC cells, suggesting that the p38 MAPK inhibitors might be effective in patients with high ING4 expression in RCC tissue. Taken together, our findings proposed that ING4 might serve as a potential therapeutic target for metastatic RCC, particularly RCC stem cells.
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http://dx.doi.org/10.3389/fphar.2022.845097DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9046557PMC
April 2022

The recombinant defensin/HSA fusion protein that inhibits NF-κb associated with intensive macropinocytosis shows potent efficacy against pancreatic cancer.

Biochem Pharmacol 2022 07 28;201:115057. Epub 2022 Apr 28.

Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China. Electronic address:

KRAS mutation and NF-κB both play crucial role in pancreatic cancer; in addition, defensin, the peptide mediator in innate immunity, can inhibit NF-κB. Assuming a strategy that targets both NF-κB and concomitantly the mutated KRAS indirectly via intensive macropinocytosis, we designed and generated a recombinant protein DF-HSA which consists of two molecules of human beta-defensin 2 (HBD2) and a moiety of human serum albumin (HSA). As shown, the recombinant protein DF-HSA markedly down-regulated NF-κB in both KRAS mutant MIA PaCa-2 cells and wild type BxPC-3 cells. Determined by confocal microscopy, the uptake of DF-HSA in MIA PaCa-2 cells was more intense than that in BxPC-3 cells. The uptake was blocked by the specific inhibitor EIPA, indicating that DF-HSA internalized via macropinocytosis. DF-HSA displayed more potent cytotoxicity to cancer cells than HBD2. DF-HSA induced apoptosis in cancer cells. Notably, DF-HSA inhibited tumor cell spheroid formation, an effect comparable to that of salinomycin. DF-HSA inhibited tumor cell migration and invasion. As detected with scanning electron microscopy, DF-HSA strongly depleted filopodia on cell surface; and salinomycin induced similar changes. By in vivo imaging, DF-HSA displayed intense tumor-site accumulation and lasting retention for over 14 days; however, HBD2 showed much less tumor-site accumulation and a shorter retention time for only 24 h. DF-HSA suppressed the growth of pancreatic cancer MIA PaCa-2 xenograft in athymic mice; and its combination with gemcitabine achieved higher antitumor efficacy. In summary, the recombinant defensin/HSA fusion protein that inhibits NF-κb associated with intensive macropinocytosis is highly effective against pancreatic cancer.
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http://dx.doi.org/10.1016/j.bcp.2022.115057DOI Listing
July 2022

Optically levitated conveyor belt based on polarization-dependent metasurface lens arrays.

Opt Lett 2022 May;47(9):2194-2197

In this Letter, we have proposed an optically levitated conveyor belt based on periodic arrays of a polarization-dependent nanoslit-based metasurface lens (NBML) that is capable of realizing far-field capture, transport, and sorting. The NBML in arrays can be lit up in a relay way by rotating the polarization angle of the excitation beam and thereby provide a better stiffness for transporting particles. When excited at the wavelength of 1064 nm and power density of 0.3 mW/µm, the particles will follow the directional movement of hot spots with an alternative switch of polarization angle and the success ratio of transport can be up to 97.0% with the consideration of Brownian motion. Furthermore, the influence of polarization switching time and incident optical power densities on the efficiency of transport are investigated numerically from a statistical point of view. The sorting of particles with different sizes has also been proved in a given power density. With the analysis of numerical results, our research provides a new approach, to the best of our knowledge, for particle trapping and transport, which is beneficial to on-chip optofluidic applications.
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http://dx.doi.org/10.1364/OL.457314DOI Listing
May 2022

Untargeted metabolomics profiling in a mouse model of lung cancer treated with thermal ablation.

Bioengineered 2022 04;13(4):11258-11268

Department of Oncology, Dongfang Hospital Beijing University of Chinese Medicine, Beijing, China.

Thermal ablation is widely used in the treatment of lung cancer and is beneficial for the overall survival of patients in clinic. However, there is barely a priority in which ablation system should be chosen under different periods of tumor progression in lung cancer. The present study investigated different modes of thermal ablation systems in mice with transplanted Lewis lung carcinoma tumors and their various biological effects in local regions using untargeted metabolomics. The results showed that thermal ablation could significantly suppress tumor growth and the differentially expressed metabolites of tumors after ablation relative to untreated tumors concentrated on organic compounds, organic acids and derivatives, nucleosides, nucleotides, and lipids. The upregulated metabolites indicated an inflammatory reaction in the ablation groups at an early stage after ablation. Steroid hormone and tryptophan metabolism, which are associated with immune responses, were modulated after both cryoablation and hyperthermal ablation. Characteristically, the results also indicated that cryoablation suppressed glucose oxidation and carbohydrate metabolism of tumor, while hyperthermal ablation suppressed lipid metabolism of tumor. In conclusion, thermal ablation could inhibit tumor growth under either freezing or heating modes with characteristic different biological effects on tumors.
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http://dx.doi.org/10.1080/21655979.2022.2065742DOI Listing
April 2022

Comparative Proteomic Analysis of Membrane Vesicles from Clinical Isolates with Differential Antibiotic Resistance.

Clin Cosmet Investig Dermatol 2022 17;15:703-712. Epub 2022 Apr 17.

Department of Dermatology, Huashan Hospital, Fudan University, Shanghai, 200040, People's Republic of China.

Purpose: () is closely associated with the pathogenesis of acne, and antibiotics targeting have been widely used for decades. However, antibiotic resistance has been increasing rapidly. Membrane vesicles (MVs) have been found to play important roles in antibiotic resistance in some bacteria. We aimed to explore the mechanism of antibiotic resistance and the virulence components within -derived MVs.

Materials And Methods: We isolated clinical strains from the lesions of acne patients who were sensitive or resistant to the antibiotics erythromycin and clindamycin. We analyzed the proteome of MVs from four sensitive isolates and three resistant isolates by LC-MS/MS.

Results: We identified 543 proteins within the MVs of clinical strains. Several lipases, NlpC/P60, CAMP factor, and Hta domain protein were detected as virulence factors in the -derived MVs. The levels of two lipases and FtsZ were significantly higher in resistant -derived MVs compared with sensitive strains (p < 0.05).

Conclusion: According to the implications of this study, improper antibiotic use might not only increase antibiotic resistance in but could also further alter the cutaneous lipid composition and aggravate host inflammation, thus resulting in worse clinical manifestations in acne patients. This study re-emphasizes that the improper use of antibiotics should be treated more seriously in clinical practice. Furthermore, to combat multidrug resistance in , this study suggests that FtsZ inhibitors could be useful.
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http://dx.doi.org/10.2147/CCID.S363537DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9022742PMC
April 2022

Corrigendum: Downregulation of GLYAT Facilitates Tumor Growth and Metastasis and Poor Clinical Outcomes Through the PI3K/AKT/Snail Pathway in Human Breast Cancer.

Front Oncol 2022 6;12:793448. Epub 2022 Apr 6.

Department of Oncology, Shengjing Hospital of China Medical University, Shenyang, China.

[This corrects the article DOI: 10.3389/fonc.2021.641399.].
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http://dx.doi.org/10.3389/fonc.2022.793448DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9020255PMC
April 2022
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