Publications by authors named "Min Ji"

473 Publications

Synovial Fluid of Patient With Rheumatoid Arthritis Enhanced Osmotic Sensitivity Through the Cytotoxic Edema Module in Synoviocytes.

Front Cell Dev Biol 2021 31;9:700879. Epub 2021 Aug 31.

Department of Physiology, College of Medicine, Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon, South Korea.

Rheumatoid arthritis (RA) is an autoimmune disease that causes inflammation of the synovial membrane ultimately leading to permanent damage in the affected joints. For this study, synovial fluids from 16 patients diagnosed with either RA or osteoarthritis (OA) were used to examine volume regulation and cooperative water channels, both of which are involved in the cytotoxic edema identified in RA-fibroblast-like synoviocytes (FLS). The osmolarity and inflammatory cytokine interleukin (IL)-6 of synovial fluids from RA patients were mildly enhanced compared to that from OA patients. RA-FLS demonstrated the enhanced property of regulatory volume increase in response to IL-6 and synovial fluids from RA patients. Although there was no difference in the protein expression of the volume-associated protein sodium-potassium-chloride cotransporter1 (NKCC1), its activity was increased by treatment with IL-6. Membrane localization of NKCC1 was also increased by IL-6 treatment. Additionally, both the protein and membrane expressions of aquaporin-1 were increased in RA-FLS by IL-6 stimulation. The IL-6-mediated enhanced osmotic sensitivity of RA-FLS likely involves NKCC1 and aquaporin-1, which mainly constitute the volume-associated ion transporter and water channel elements. These results suggest that RA-FLS provide enhanced electrolytes and concomitant water movement through NKCC1 and aquaporin-1, thereby inducing cellular swelling ultimately resulting in cytotoxic edema. Attenuation of cytotoxic edema and verification of its related mechanism will provide novel therapeutic approaches to RA treatment within the scope of cytotoxic edema.
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http://dx.doi.org/10.3389/fcell.2021.700879DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8438158PMC
August 2021

Morphological changes in the trabecular meshwork and Schlemm's canal after treatment with topical intraocular pressure-lowering agents.

Sci Rep 2021 Sep 13;11(1):18169. Epub 2021 Sep 13.

Department of Ophthalmology, Korea University Ansan Hospital, 123 Jeokgeum-ro, Danwon-gu, Ansan-si, Gyeonggi-do, 15355, South Korea.

Glaucoma treatment is usually initiated with topical medication that lowers the intraocular pressure (IOP) by reducing the aqueous production, enhancing the aqueous outflow, or both. However, the effect of topical IOP-lowering medications on the microstructures of the aqueous outflow pathway are relatively unknown. In this retrospective, observational study, 56 treatment-naïve patients with primary open-angle glaucoma were enrolled. Images of the nasal and temporal corneoscleral limbus were obtained using anterior segment optical coherence tomography (AS-OCT). The conjunctival vessels and iris anatomy were used as landmarks to select the same limbal area scan, and the trabecular meshwork (TM) width, TM thickness, and Schlemm's canal (SC) area were measured before and after using the IOP-lowering agents for 3 months. Among the 56 patients enrolled, 33 patients used prostaglandin (PG) analogues, and 23 patients used dorzolamide/timolol fixed combination (DTFC). After 3 months of DTFC usage, the TM width, TM thickness, and SC area did not show significant changes in either the nasal or temporal sectors. Conversely, after prostaglandin analog usage, the TM thickness significantly increased, and the SC area significantly decreased (all P < 0.01). These findings warrant a deeper investigation into their relationship to aqueous outflow through the conventional and unconventional outflow pathways after treatment with PG analogues.
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http://dx.doi.org/10.1038/s41598-021-97746-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8437975PMC
September 2021

Percutaneous radiofrequency ablation for hepatic metastasis of colorectal cancer: assessment of tumor visibility and the feasibility of the procedure with planning ultrasonography.

Ultrasonography 2021 Jun 22. Epub 2021 Jun 22.

Department of Radiology and Center for Imaging Science, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.

Purpose: The aim of this study was to assess the incidence and causes of percutaneous radiofrequency ablation (RFA) infeasibility in cases of metastatic colorectal cancer and to evaluate factors affecting the invisibility of the tumor on planning ultrasonography (US).

Methods: This study screened 386 patients who underwent planning US using fusion imaging and/or contrast-enhanced US for percutaneous RFA for suspected metastatic colorectal cancer between January 2013 and December 2020, from whom 136 patients with a single hepatic metastasis from colorectal cancer measuring <3 cm were included. The factors related to the infeasibility of percutaneous RFA were investigated. Univariate and multivariate analyses were performed to assess the factors associated with tumor invisibility on planning US.

Results: Among the 136 patients, percutaneous RFA was considered infeasible in 24.3% (33/136) due to a high risk of the heat-sink effect caused by the abutment of a large vessel (n=12), an inconspicuous tumor on planning US (n=11), a high risk of collateral thermal damage to an adjacent organ (n=8), and the absence of a safe electrode path (n=2). In univariate and multivariate analyses, tumor size was a statistically significant factor affecting invisibility on planning US (P=0.003 and P=0.018, respectively).

Conclusion: Percutaneous RFA was infeasible in approximately one-fourth of patients with metastatic colorectal cancer. The reason for the infeasibility was mainly an unfavorable tumor location and invisibility on planning US. Small tumor size was the sole significant factor affecting the invisibility of hepatic metastases on planning US.
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http://dx.doi.org/10.14366/usg.21050DOI Listing
June 2021

Treatment Effect and Pain During Treatment With Intense Pulsed-Light Therapy According to the Light Guide in Patients With Meibomian Gland Dysfunction.

Cornea 2021 Aug 30. Epub 2021 Aug 30.

Department of Ophthalmology, Institute of Vision Research, Yonsei University College of Medicine, Seoul, South Korea; and Department of Ophthalmology, Cornea Dystrophy Research Institute, Yonsei University College of Medicine, Seoul, South Korea.

Purpose: We investigated whether there is a difference in the treatment effect and pain during the treatment of meibomian gland dysfunction (MGD) with intense pulsed-light (IPL) between new light guide and conventional light guide.

Methods: We retrospectively reviewed medical records of 85 patients (170 eyes) who underwent IPL treatment of the upper and lower eyelids 3 times, at 3-week intervals, for MGD. Patients treated with the 6-mm or 8 × 15-mm cylindrical light guide were designated as group A or group B, respectively. The ocular surface disease index (OSDI), dry eye (DE), and MGD parameters were obtained before the first and after the third IPL treatments. Visual analog scale (VAS) scores were obtained at every IPL treatment. OSDI, DE, and MGD parameters and VAS scores were compared between the groups.

Results: VAS scores at the first, second, and third IPL treatments were lower in group A than in group B. OSDI, DE, and MGD parameters were improved after 3 IPL treatments in both groups. There were no significant differences in OSDI, DE symptoms, and MGD parameters between before the first IPL treatment and after the third IPL treatment between the groups.

Conclusions: Using the new 6-mm cylindrical light guide for IPL treatment in patients with MGD induced less pain during treatment and had similar treatment effects to the conventional 8 × 15-mm light guide. The new 6-mm cylindrical light guide can be useful when treating patients with dark or hyperpigmented skin and for pediatric patients with low compliance.
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http://dx.doi.org/10.1097/ICO.0000000000002859DOI Listing
August 2021

Effect of exogenous N-acyl-homoserine lactones on the anammox process at 15 ℃: Nitrogen removal performance, gene expression and metagenomics analysis.

Bioresour Technol 2021 Aug 13;341:125760. Epub 2021 Aug 13.

School of Environmental Science and Engineering, Tianjin University, Tianjin 300350, China.

In this study, C6-HSL and C8-HSL were separately introduced into anammox biofilm reactors to facilitate the anammox performance at 15 ℃. After operation 138 d, total nitrogen removal efficiencies in reactors with amendment C6-HSL or C8-HSL at 15 ℃ reached 76.2% and 74.6%, respectively. Content of extracellular polymeric substances increased by 19.8%, 67.7% and 121.2% in control group, C6-HSL and C8-HSL addition group, respectively. Genes associated with nitrogen removal (i.e., hzo, hzsB, nirS, and ccsB) showed higher expression level at amendment C6-HSL or C8-HSL group. Metagenomics analysis found that amendment of C6-HSL or C8-HL resulted in an increased abundance of genes related to the tricarboxylic acid cycle, amino sugar and nucleotide sugar metabolism, and also genes associated with amino acid biosynthesis pathways. Overall, amendment C6-HSL or C8-HSL had been confirmed as the effective method to improve the performance of anammox bioreactor at 15 ℃.
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http://dx.doi.org/10.1016/j.biortech.2021.125760DOI Listing
August 2021

Effects of Photobiomodulation on Changes in Cognitive Function and Regional Cerebral Blood Flow in Patients with Mild Cognitive Impairment: A Pilot Uncontrolled Trial.

J Alzheimers Dis 2021 Aug 14. Epub 2021 Aug 14.

Research Institute for Convergence of Biomedical Science and Technology, Pusan National University Yangsan Hospital, Yangsan, Republic of Korea.

Background: Photobiomodulation (PBM) affects local blood flow regulation through nitric oxide generation, and various studies have reported on its effect on improving cognitive function in neurodegenerative diseases. However, the effect of PBM in the areas of the vertebral arteries (VA) and internal carotid arteries (ICA), which are the major blood-supplying arteries to the brain, has not been previously investigated.

Objective: We aimed to determine whether irradiating PBM in the areas of the VA and ICA, which are the major blood-supplying arteries to the brain, improved regional cerebral blood flow (rCBF) and cognitive function.

Methods: Fourteen patients with mild cognitive impairments were treated with PBM. Cognitive assessment and single-photon emission computed tomography were implemented at the baseline and at the end of PBM.

Results: Regarding rCBF, statistically significant trends were found in the medial prefrontal cortex, lateral prefrontal cortex, anterior cingulate cortex, and occipital lateral cortex. Based on the cognitive assessments, statistically significant trends were found in overall cognitive function, memory, and frontal/executive function.

Conclusion: We confirmed the possibility that PBM treatment in the VA and ICA areas could positively affect cognitive function by increasing rCBF. A study with a larger sample size is needed to validate the potential of PBM.
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http://dx.doi.org/10.3233/JAD-210386DOI Listing
August 2021

Prognosis after Curative Resection of Single Hepatocellular Carcinoma with A Focus on LI-RADS Targetoid Appearance on Preoperative Gadoxetic Acid-Enhanced MRI.

Korean J Radiol 2021 Jul 26. Epub 2021 Jul 26.

Biostatics and Clinical Epidemiology Center, Research Institute for Future Medicine, Samsung Medical Center, Seoul, Korea.

Objective: To evaluate the prognostic implications of preoperative magnetic resonance imaging (MRI) features of hepatocellular carcinoma (HCC) with a focus on those with targetoid appearance based on the Liver Imaging Reporting and Data System (LI-RADS), as well as known microvascular invasion (MVI) features.

Materials And Methods: This retrospective study included 242 patients (190 male; mean age, 57.1 years) who underwent surgical resection of a single HCC (≤ 5 cm) as well as preoperative gadoxetic acid-enhanced MRI between January 2012 and March 2015. LI-RADS category was assigned, and the LR-M category was further classified into two groups according to rim arterial-phase hyperenhancement (APHE). The imaging features associated with MVI were also assessed. The overall survival (OS), recurrence-free survival (RFS), and their associated factors were evaluated.

Results: Among the 242 HCCs, 190 (78.5%), 25 (10.3%), and 27 (11.2%) were classified as LR-4/5, LR-M with rim APHE, and LR-M without rim APHE, respectively. LR-M with rim APHE (vs. LR-4/5; hazard ratio [HR] for OS, 5.48 [ = 0.002]; HR for RFS, 2.09 [ = 0.042]) and tumor size (per cm increase; HR for OS, 6.04 [ = 0.009]; HR for RFS, 1.77 [ = 0.014]) but not MVI imaging features ( > 0.05) were independent factors associated with OS and RFS. Compared to the 5-year OS and RFS rates in the LR-4/5 group (93.9% and 66.8%, respectively), the LR-M with rim APHE group had significantly lower rates (68.0% and 45.8%, respectively, both < 0.05), while the LR-M without rim APHE group did not significantly differ in the survival rates (91.3% and 80.2%, respectively, both > 0.05).

Conclusion: Further classification of LR-M according to the presence of rim APHE may help predict the postoperative prognosis of patients with a single HCC.
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http://dx.doi.org/10.3348/kjr.2020.1428DOI Listing
July 2021

Development and Validation of Unplanned Extubation Prediction Models Using Intensive Care Unit Data: Retrospective, Comparative, Machine Learning Study.

J Med Internet Res 2021 Aug 11;23(8):e23508. Epub 2021 Aug 11.

Department of Digital Health, Samsung Advanced Institute for Health Sciences & Technology, Sungkyunkwan University, Seoul, Republic of Korea.

Background: Patient safety in the intensive care unit (ICU) is one of the most critical issues, and unplanned extubation (UE) is considered the most adverse event for patient safety. Prevention and early detection of such an event is an essential but difficult component of quality care.

Objective: This study aimed to develop and validate prediction models for UE in ICU patients using machine learning.

Methods: This study was conducted in an academic tertiary hospital in Seoul, Republic of Korea. The hospital had approximately 2000 inpatient beds and 120 ICU beds. As of January 2019, the hospital had approximately 9000 outpatients on a daily basis. The number of annual ICU admissions was approximately 10,000. We conducted a retrospective study between January 1, 2010, and December 31, 2018. A total of 6914 extubation cases were included. We developed a UE prediction model using machine learning algorithms, which included random forest (RF), logistic regression (LR), artificial neural network (ANN), and support vector machine (SVM). For evaluating the model's performance, we used the area under the receiver operating characteristic curve (AUROC). The sensitivity, specificity, positive predictive value, negative predictive value, and F1 score were also determined for each model. For performance evaluation, we also used a calibration curve, the Brier score, and the integrated calibration index (ICI) to compare different models. The potential clinical usefulness of the best model at the best threshold was assessed through a net benefit approach using a decision curve.

Results: Among the 6914 extubation cases, 248 underwent UE. In the UE group, there were more males than females, higher use of physical restraints, and fewer surgeries. The incidence of UE was higher during the night shift as compared to the planned extubation group. The rate of reintubation within 24 hours and hospital mortality were higher in the UE group. The UE prediction algorithm was developed, and the AUROC for RF was 0.787, for LR was 0.762, for ANN was 0.763, and for SVM was 0.740.

Conclusions: We successfully developed and validated machine learning-based prediction models to predict UE in ICU patients using electronic health record data. The best AUROC was 0.787 and the sensitivity was 0.949, which was obtained using the RF algorithm. The RF model was well-calibrated, and the Brier score and ICI were 0.129 and 0.048, respectively. The proposed prediction model uses widely available variables to limit the additional workload on the clinician. Further, this evaluation suggests that the model holds potential for clinical usefulness.
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http://dx.doi.org/10.2196/23508DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8387891PMC
August 2021

Design, synthesis and application of near-infrared fluorescence probe IR-780-Crizotinib in detection of ALK positive tumors.

Protein Expr Purif 2021 Nov 7;187:105952. Epub 2021 Aug 7.

College of Biological and Environmental Engineering, Shandong Provincial Engineering and Technology Research Center for Wild Plant Resources Development and Application of Yellow River Delta, Binzhou University, Binzhou, 256603, China.

At present, the early diagnosis and treatment of NSCLC has become an international research hotspot. However, how to realize the organic combination of highly sensitive and high-resolution tumor imaging diagnosis and effective treatment, and to provide effective information for the diagnosis and treatment of cancer is still a major problem in the integration of cancer diagnosis and treatment. In this study, based on the Crizotinib has a good targeted inhibitory effect on ALK positive tumor cells, the near-infrared targeted fluorescent dye IR-780 was covalently bound with the drug molecule Crizotinib, thus the near-infrared fluorescent probe IR-780-Crizotinib targeting ALK positive tumor cells was synthesized. The probe structure is confirmed by NMR and MS. The optical properties of the fluorescent probe and the imaging process in ALK positive tumor-bearing mice were analyzed using ultraviolet spectrophotometer, near-infrared fluorescence spectrometer, and near-infrared fluorescence imaging system. The results show that the probe had better photoactivity. In vivo imaging shows that the probe maintained the biological activity of Crizotinib, effectively targeting the tumor site involved with clear imaging, and ultimately excreted from the body. It was confirmed that the probe could be used for the tracking, positioning and targeted therapy of nude mice with ALK positive tumors in vivo, thus exploring a new approach for the clinical application of near-infrared fluorescent probe to detect ALK positive tumors in the future.
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http://dx.doi.org/10.1016/j.pep.2021.105952DOI Listing
November 2021

A modified LI-RADS: targetoid tumors with enhancing capsule can be diagnosed as HCC instead of LR-M lesions.

Eur Radiol 2021 Aug 4. Epub 2021 Aug 4.

Department of Mathematics, Ajou University, Suwon, Republic of Korea.

Objective: To elucidate whether the presence of enhancing capsule can be applied to establish a modified Liver Imaging Reporting and Data System (LI-RADS) to differentiate hepatocellular carcinoma (HCC) from non-HCC malignancies in extracellular contrast agent (ECA)-enhanced and hepatobiliary agent (HBA)-enhanced MRI.

Methods: We enrolled 198 participants (161 men; mean age, 56.3 years) with chronic liver disease who underwent ECA-MRI and HBA-MRI before surgery for de novo hepatic nodule(s). Two reviewers assigned LI-RADS categories (v2018). We defined a "modified LR-5 category, which emphasizes enhancing capsule (mLR-5C)" over targetoid features and classifies tumors with both targetoid appearance and enhancing capsule as HCC instead of LR-M. We compared the diagnostic performance of conventional LI-RADS and modified LI-RADS criteria for both MRIs.

Results: A total of 258 hepatic nodules (194 HCCs, 43 benign lesions, and 21 non-HCC malignancies; median size, 19 mm) were analyzed. By conventional LI-RADS, 47 (18.2%) nodules (31 HCCs and 16 non-HCC malignancies) were categorized as LR-M. The mLR-5C criterion showed superior sensitivity (ECA-MRI, 76.6% vs. 67.0%; HBA-MRI, 60.4% vs. 56.3%; both p < 0.05) while maintaining high specificity (ECA-MRI, 93.8% vs. 98.4%; HBA-MRI, 95.3% vs. 98.4%; both p > 0.05) compared with the LR-5 criterion. Using the mLR-5C criterion, ECA-MRI exhibited higher sensitivity than HBA-MRI (76.6% vs. 60.4%, p < 0.001) and similar specificity (93.8% vs. 95.3%, p > 0.99).

Conclusion: Our modified LI-RADS achieved superior sensitivity for diagnosing HCC, without compromising specificity compared with LR-5. ECA-MRI showed higher sensitivity in diagnosing HCC than HBA-MRI by applying the mLR-5C for LR-M lesions.

Key Points: • By conventional LI-RADS, 31 (16.0%) of 194 HCCs were categorized as LR-M. • Among 31 HCCs categorized as LR-M, 19 HCCs or 8 HCCs were recategorized as HCC on ECA-MRI or HBA-MRI, respectively, after applying the modified LR-5 category, which allocates targetoid lesions with enhancing capsule as mLR-5C instead of LR-M. • The mLR-5C showed superior sensitivity compared with the LR-5 in both MRIs (ECA-MRI, 76.6% vs. 67.0%; HBA-MRI, 60.4% vs. 56.3%, both p < 0.05), while maintaining high specificity (ECA-MRI, 93.8% vs. 98.4%; HBA-MRI, 95.3% vs. 98.4%; both p > 0.05).
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http://dx.doi.org/10.1007/s00330-021-08124-0DOI Listing
August 2021

Woodchips bioretention column for stormwater treatment: Nitrogen removal performance, carbon source and microbial community analysis.

Chemosphere 2021 Jul 9;285:131519. Epub 2021 Jul 9.

School of Environmental Science and Engineering, Tianjin University, Tianjin, 300350, China.

This study chose Oak woodchips and gravel as media filter to enhance the denitrification in the bioretention system (saturated zone 7.7 L) treating synthetic stormwater runoff. It revealed that the denitrification process mainly occurred during the drying phase and enlarging volume of saturated zones to retain more stormwater during storm event was the direct method to promote nitrogen removal of the bioretention system. Nevertheless, it was noted that the nitrogen and dissolved organic carbon would be released into the effluent during the wetting period. The denitrification rate with different nitrate nitrogen (NO-N) concentrations did not show the obvious change with zero order kinetics constant of 2.91 mg/L∙d on average. Furthermore, it confirmed that woodchips were degraded and converted to volatile fatty acids (VFAs), especially acetic acid as carbon source, further utilized by the denitrifying bacteria, such as Dechloromonas, Acidoborax, Pseudomonas, Denitratisoma and Acinetobacter. In addition, genera of Lachnospiraceae and Lactobacillus, which had the ability to degrade the macromolecular organic components into low molecular VFAs, were observed in the woodchips bioretention system.
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http://dx.doi.org/10.1016/j.chemosphere.2021.131519DOI Listing
July 2021

Application of different redox mediators induced bio-promoters to accelerate the recovery of denitrification and denitrifying functional microorganisms inhibited by transient Cr(VI) shock.

J Hazard Mater 2021 Jul 17;420:126664. Epub 2021 Jul 17.

School of Environmental Science and Engineering, Tianjin University, Tianjin 300350, China.

The transient hexavalent chromium (Cr(VI)) shock may directly inhibit the denitrification process of municipal wastewater treatment plants (WWTPs), which is difficult to recover in a short time. This study developed four nontoxic bio-promoters (combination of L-cysteine, flavin adenine dinucleotide (FAD), biotin, cytokinin and different redox mediators) to quickly restore the denitrification performance after high-loading Cr(VI) suppressing. After feeding with 100 mg/L of Cr(VI) for 42 cycles (T, 4 h), the removal efficiency of nitrate was reduced by 85.00%, and nitrite was accumulated simultaneously. The denitrification performance was recovered quickly with the addition of bio-promoters, introducing redox mediators showed noticeable superiority on the bio-inhibition release. Compared with sodium humate and riboflavin, the AQDS induced bio-promoter achieved the best nitrate removal recovery performance within only 28 T, and the recovery rate was 2.16 times faster than the natural recovery. Microbial analysis showed that Cr(VI) specially inhibited napA-type denitrifiers, and the OTU numbers sharply dropped by 48.74%. Redox mediators induced bio-promoters could effectively recover the abundance of napA-type and nirS-type denitrifying microorganisms, which was consistent with the change of nitrate removal efficiency. This study offers a cost-effective approach to deal with Cr(VI) shock problem, which may promote the development of bio-promoters for WWTPs.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126664DOI Listing
July 2021

PAK1 Mediates Bone Marrow Stromal Cell-Induced Drug Resistance in Acute Myeloid Leukemia via ERK1/2 Signaling Pathway.

Front Cell Dev Biol 2021 8;9:686695. Epub 2021 Jul 8.

Department of Hematology, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, China.

Background: Chemoresistance is emerging as a major barrier to successful treatment in acute myeloid leukemia (AML), and bone marrow stromal cells (BMSCs) protect leukemia cells from chemotherapy eventually leading to recurrence. This study was designed to investigate the role of p21-activated kinase 1 (PAK1) in AML progression and chemosensitivity, highlighting the mechanism of stroma-mediated chemoresistance.

Methods: The GEPIA and TCGA datasets were used to analyze the relationship between PAK1 mRNA expression and various clinical parameters of AML patients. Cell proliferation and apoptosis were examined to evaluate the role of PAK1 on chemosensitivity in AML by silencing PAK1 with shRNA or small molecular inhibitor. Human BMSC (HS-5) was utilized to mimic the leukemia bone marrow microenvironment (BMM) , and co-culture model was established to investigate the role of PAK1 in BMSC-mediated drug resistance.

Results: p21-activated kinase 1 high expression was shown to be associated with shorter overall survival in AML patients. The silence of PAK1 could repress cell proliferation, promote apoptosis, and enhance the sensitivity of AML cells to chemotherapeutic agents. More importantly, BMSCs induced PAK1 up-regulation in AML cells, subsequently activating the ERK1/2 signaling pathway. The effect of BMSC-mediated apoptotic-resistance could be partly reversed by knock down of PAK1.

Conclusion: p21-activated kinase 1 is a potential prognostic predictor for AML patients. PAK1 may play a pivotal role in mediating BMM-induced drug resistance, representing a novel therapeutic target in AML.
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http://dx.doi.org/10.3389/fcell.2021.686695DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8297649PMC
July 2021

Binary Surfactant-Mediated Tunable Nanotip Growth on Gold Nanoparticles and Applications in Photothermal Catalysis.

Front Chem 2021 7;9:699548. Epub 2021 Jul 7.

School of Physics and Information Technology, Shaanxi Normal University, Xi'an, China.

Plasmonic nanostructures with sharp tips are widely used for optical signal enhancement because of their strong light-confining abilities. These structures have a wide range of potential applications, for example, in sensing, bioimaging, and surface-enhanced Raman scattering. Au nanoparticles, which are important plasmonic materials with high photothermal conversion efficiencies in the visible to near-infrared region, have contributed greatly to the development of photothermal catalysis. However, the existing methods for synthesizing nanostructures with tips need the assistance of poly(vinylpyrrolidone), thiols, or biomolecules. This greatly hinders signal detection because of stubborn residues. Here, we propose an efficient binary surfactant-mediated method for controlling nanotip growth on Au nanoparticle surfaces. This avoids the effects of surfactants and can be used with other Au nanostructures. The Au architecture tip growth process can be controlled well by adjusting the ratio of hexadecyltrimethylammonium bromide to hexadecyltrimethylammonium chloride. This is due to the different levels of attraction between Br/Cl and Au ions. The surface-enhanced Raman scattering and catalytic abilities of the synthesized nanoparticles with tips were evaluated by electromagnetic simulation and photothermal catalysis experiments (with 4-nitrothiophenol). The results show good potential for use in surface-enhanced Raman scattering applications. This method provides a new strategy for designing plasmonic photothermal nanostructures for chemical and biological applications.
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http://dx.doi.org/10.3389/fchem.2021.699548DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8294035PMC
July 2021

Design, synthesis and preliminary bioactivity evaluation of bitopic benzopyranomorpholine analogues as selective dopamine D3 receptor ligands as anti-drug addiction therapeutic agents.

Bioorg Med Chem Lett 2021 Sep 17;48:128269. Epub 2021 Jul 17.

School of Biological Science & Medical Engineering, Southeast University, Nanjing 210096, PR China.

Three series of bitopic benzopyranomorpholine analogues were designed, synthesized, and evaluated as a novel class of selective ligands for the dopamine D3 receptor. Binding affinities of target compounds were determined using the method of radioligand binding assay. Most compounds demonstrated considerable binding affinities and selectivity for D3 receptor. Besides, the compounds were screened for their ability to alleviate withdrawal symptoms of opioid addiction in animal behavioral models. The results showed that compound 20h displayed nanomolar affinity for the D3R, and exhibited anti-drug addiction efficacy in the animal model of of naloxone-induced withdrawal symptoms in morphine-dependent mice.
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http://dx.doi.org/10.1016/j.bmcl.2021.128269DOI Listing
September 2021

Occurrence of antibiotics and antibiotic resistance genes in WWTP effluent-receiving water bodies and reclaimed wastewater treatment plants.

Sci Total Environ 2021 Nov 9;796:148919. Epub 2021 Jul 9.

North China Municipal Engineering Design & Research Institute Co., LTD, Olympic Road, 300381, China.

There is a growing concern on the fate and the consequent ecological or health risks of antibiotics and antibiotic resistance genes (ARGs) in natural or artificial water environment. The effluent of wastewater treatment plants (WWTPs) has been reported to be an important source of antibiotics and ARGs in the environment. WWTP effluent could be discharged into surface water bodies or recycled, either of which could lead to different exposure risks. The impact of WWTP effluents on the levels of antibiotics and ARGs in effluent-receiving water bodies and the removal efficiency of antibiotics and ARGs in reclaimed wastewater treatment plants (RWTPs) were seldom simultaneously investigated. Thus, in this study, we investigated the occurrence of antibiotics and ARGs in four WWTP effluents, and their downstream effluent-receiving water bodies and RWTPs in seasons of low-water-level. The total concentrations of ofloxacin, norfloxacin, ciprofloxacin, roxithromycin, azithromycin, erythromycin, tetracycline, oxytetracycline, chlortetracycline, and sulfamethoxazole in the secondary effluents were 1441.6-4917.6 ng L. Ofloxacin had the highest concentration. The absolute and relative abundances of total ARGs (qnrD, qnrS, ermA, ermB, tetA, tetQ, sul1, and sul2) in the secondary effluents were 10-10 copies mL and 10-10 ARG/16S rRNA. Sul1 and sul2 were the major species with the highest detection frequencies and levels. In most cases, WWTP effluents were not the major contributors to the levels and species of antibiotics and ARGs in the surface water bodies. Four RWTPs removed 43.5-98.9% of antibiotics and - 0.19-2.91 log of ARGs. Antibiotics and ARGs increased in chlorination, ozonation and filtration units. Antibiotics had significantly positive correlations with ARGs, biological oxygen demands, total phosphorus, total nitrogen, and ammonia nitrogen in the four effluent-receiving water bodies. In RWTPs, the total concentrations of antibiotics showed a significant positive correlation with the total abundance of ARGs.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148919DOI Listing
November 2021

Prediction of hypotension after postural change in robot-assisted laparoscopic prostatectomy using esophageal Doppler monitoring: a prospective observational trial.

Sci Rep 2021 Jul 16;11(1):14589. Epub 2021 Jul 16.

Department of Anesthesiology and Pain Medicine, Anesthesia and Pain Research Institute, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 03722, Republic of Korea.

Postural change from a steep Trendelenburg position to a supine position (T-off) during robot-assisted laparoscopic prostatectomy (RALP) induces a considerable abrupt decrease in the mean arterial pressure (MAP). We investigated the variables for predicting postural hypotension induced by T-off using esophageal Doppler monitoring (EDM). One hundred and twenty-five patients undergoing RALP were enrolled. Data on the MAP, heart rate, stroke volume index (SVI), cardiac index, peak velocity, corrected flow time, stroke volume variation, pulse pressure variation, arterial elastance (Ea), and dynamic arterial elastance were collected before T-off and at 1, 3, 5, 7, and 10 min after T-off using EDM. MAP < 60 mmHg within 10 min after T-off was considered to indicate hypotension, and 25 patients developed hypotension. The areas under the curves of the MAP, SVI, and Ea were 0.734 (95% confidence interval [CI] 0.623-0.846; P < 0.001), 0.712 (95% CI 0.598-0.825; P < 0.001), and 0.760 (95% CI 0.646-0.875; P < 0.001), respectively, with threshold values of ≤ 74 mmHg, ≥ 42.5 mL/m, and ≤ 1.08 mmHg/mL, respectively. If patients have MAP < 75 mmHg with SVI ≥ 42.5 mL/m or Ea ≤ 1.08 mmHg/mL before postural change from T-off during RALP, prompt management for ensuing hypotension should be considered.Trial registration: NCT03882697 (ClinicalTrial.gov, March 20, 2019).
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http://dx.doi.org/10.1038/s41598-021-93990-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8285389PMC
July 2021

Abnormal interhemispheric resting state functional connectivity in Parkinson's disease patients with impulse control disorders.

NPJ Parkinsons Dis 2021 Jul 16;7(1):60. Epub 2021 Jul 16.

Department of Neurology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Impulse control disorders (ICD) in Parkinson's disease (PD) might be attributed to misestimate of rewards or the failure to curb inappropriate choices. The mechanisms underlying ICD were reported to involve the lateralization of monoamine network. Our objective was to probe the significant role of lateralization in the pathogenesis of ICD. Twenty-one PD patients with ICD (PD-ICD), thirty-three without ICD (PD-no ICD), and thirty-seven healthy controls (HCs) were recruited and performed T1-weighted, diffusion tensor imaging (DTI) scans and resting state functional magnetic resonance imaging (rs-fMRI). By applying the Voxel-mirrored Homotopic Connectivity (VMHC) and Freesurfer, we evaluated participants' synchronicity of functional connectivity and structural changes between hemispheres. Also, tract-based spatial statistics (TBSS) was applied to compare fiber tracts differences. Relative to PD-no ICD group, PD-ICD group demonstrated reduced VMHC values in middle frontal gyrus (MFG). Compared to HCs, PD-ICD group mainly showed decreased VMHC values in MFG, middle and superior orbital frontal gyrus (OFG), inferior frontal gyrus (IFG) and caudate, which were related to reward processing and inhibitory control. The severity of impulsivity was negatively correlated with the mean VMHC values of MFG in PD-ICD group. Receiver operating characteristic (ROC) curves analyses uncovered that the mean VMHC values of MFG might be a potential marker identifying PD-ICD patients. However, we found no corresponding asymmetrical alteration in cortical thickness and no significant differences in fractional anisotropy (FA) and mean diffusivity (MD). Our results provided further evidence for asymmetry of functional connectivity in mesolimbic reward and response inhibition network in ICD.
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http://dx.doi.org/10.1038/s41531-021-00205-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8285494PMC
July 2021

Effects of water flow on performance of soil microbial fuel cells: Electricity generation, benzo[a]pyrene removal, microbial community and molecular ecological networks.

Environ Res 2021 Jul 10;202:111658. Epub 2021 Jul 10.

School of Environmental Science and Engineering, Tianjin University, Tianjin, 300350, China.

Soil microbial fuel cells with water flow (W-SMFCs) as a driven force of substrate transport were constructed. Electricity generation, benzo[a]pyrene (BaP) removal, microbial communities and microbial molecular ecological networks were compared between W-SMFCs and their control reactors (without water flow, C-SMFCs) in 240 days of operation. The W-SMFCs started up faster than C-SMFCs (37 days vs. 50 days) and output higher startup voltage (148.45 mV vs. 111.90 mV). The water flow caused higher removal efficiency of BaP at sites >1 cm from the anode (S ) than at sites <1 cm from the anode (S ) in W-SMFCs, whereas in C-SMFCs, the removal efficiency of BaP at S was higher than that at S. The removal efficiency of BaP at S in W-SMFCs was up to 1.7 times higher than that at S in C-SMFCs on the 91 day. After 240 days of operation, the biodegradation efficiency of absolute BaP amount was 45.95% in W-SMFCs, being 20% higher than that in C-SMFCs (38.17%). Moreover, the water flow caused highly tight interaction among the microbial species, which could be beneficial to BaP biodegradation. Conclusively, the water flow in soil was very beneficial for startup and biodegradation of BaP in SMFCs.
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http://dx.doi.org/10.1016/j.envres.2021.111658DOI Listing
July 2021

Enhanced membrane fouling control and trace organic compounds removal during microfiltration by coupling coagulation and adsorption in an electric field.

Sci Total Environ 2021 Nov 2;795:148830. Epub 2021 Jul 2.

School of Environmental Science and Engineering, Tianjin University, Tianjin 300350, PR China. Electronic address:

Coupling electric field, coagulation, and powdered activated carbon (PAC) adsorption in the microfiltration (MF) process was an effective strategy for membrane fouling alleviation and trace organic compounds (TrOCs) elimination. In the electric field, the surface charges of bovine serum albumin (BSA) molecules and kaolin particles distributed along the direction of the electric field and formed electric dipoles, which lowered electrostatic repulsion between BSA-BSA, BSA-kaolin, and kaolin-kaolin, resulting in enhanced particle aggregation and turbidity reduction. Electrophoretic migration also strengthened the interaction between particles and polyaluminum chloride (PACl). Protein removal showed a significant linear correlation with the transmembrane pressure (TMP), and the slope variation of the fitting curves reflected the role of the electric field in the BSA removal and membrane fouling control. Under the synergistic function of electric field, coagulation, and PAC adsorption, the MF system achieved an 80.7% reduction on membrane fouling, an average BSA removal of 76.4%, and TrOCs elimination of 65.3%-81.7%. Electrochemical oxidation was the main contributor to the TrOCs removal when 2.5 V voltage was applied, and could also prolong the service life of PAC.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148830DOI Listing
November 2021

EASL versus LI-RADS: Intra-individual comparison of MRI with extracellular contrast and gadoxetic acid for diagnosis of small HCC.

Liver Int 2021 Jul 9. Epub 2021 Jul 9.

Department of Mathematics, Ajou University, Suwon, Republic of Korea.

Background & Aims: Liver Imaging Reporting and Data System (LI-RADS) and European Association for the Study (EASL) criteria for hepatocellular carcinoma (HCC) diagnosis have been updated in 2018. We aimed to compare the HCC diagnostic performance of LI-RADS and EASL criteria with extracellular contrast agents-MRI (ECA-MRI) and hepatobiliary agents-MRI (HBA-MRI).

Methods: We prospectively evaluated 179 participants with cirrhosis (n = 105) or non-cirrhotic chronic hepatitis B (CHB) (n = 74) who underwent both ECA-MRI and HBA-MRI before surgery for de novo nodule(s) measuring 10-30 mm. We compared the HCC diagnostic performance of EASL and LR-5 in both MRIs.

Results: In an analysis of 215 observations (175 HCCs, 17 non-HCC malignancies and 23 benign lesions) identified from cirrhotic or non-cirrhotic CHB participants, LR-5 with ECA-MRI provided the highest sensitivity (80.7%), followed by EASL with ECA-MRI (76.2%), LR-5 with HBA-MRI (67.3%) and EASL with HBA-MRI (63.0%, all P < .05). The specificities were comparable (89.4%-91.5%). When the analysis is limited to participants with pathological cirrhosis (123 observations), the sensitivity of LR-5 with ECA-MRI was similar to that of EASL with ECA-MRI (82.7% vs 80.2%, P = .156), but higher than LR-5 with HBA-MRI (65.1%) or EASL with HBA-MRI (62.8%, both P < .001), with comparable specificities (87.5%-91.7%).

Conclusions: The LR-5 with ECA-MRI yielded the highest sensitivity with a similar specificity for HCC diagnosis in cirrhosis and non-cirrhotic CHB participants, while the sensitivities of LR-5 and EASL with ECA-MRI are similar for cirrhosis participants. This indicates non-invasive diagnosis criteria can differ by contrast agents and presence of cirrhosis.
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http://dx.doi.org/10.1111/liv.15012DOI Listing
July 2021

Design, Synthesis and Activity Evaluation of New Phthalazinone PARP Inhibitors.

Chem Pharm Bull (Tokyo) 2021 ;69(7):620-629

School of Chemistry and Chemical Engineering, Southeast University.

Poly(ADP-ribose)polymerase (PARP) is a significant therapeutic target for the treatment of numerous human diseases. Olaparib has been approved as a PARP inhibitor. In this paper, a series of new compounds were designed and synthesized with Olaparib as the lead compound. In order to evaluate the inhibitory activities against PARP1 of the synthesized compounds, in vitro PARP1 inhibition assay and intracellular PARylation assay were conducted. The results showed that the inhibitory activities of the derivatives were related to the type of substituent and the length of alkyl chain connecting the aromatic ring. 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT)-based assay also proved that these compounds demonstrating strong inhibition to PARP1 also have high anti-proliferative activities against BRCA2-deficient cell line (Capan-1). Analysis of the entire results suggest that compound 23 with desirable inhibitory efficiency may hold promise for further in vivo exploration of PARP inhibition.
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http://dx.doi.org/10.1248/cpb.c20-01018DOI Listing
August 2021

Oxygen-generating glycol chitosan-manganese dioxide nanoparticles enhance the photodynamic effects of chlorin e6 on activated macrophages in hypoxic conditions.

Int J Biol Macromol 2021 Aug 10;184:20-28. Epub 2021 Jun 10.

Department of Systems Biotechnology, Chung-Ang University, Anseong-si, Gyeonggi-do 17546, Republic of Korea. Electronic address:

This study aimed to investigate the use of glycol chitosan (GC) for the synthesis of MnO nanoparticles (NPs) and to evaluate whether the prepared GC-MnO NPs enhance the light-triggered photodynamic effects of chlorin e6 (Ce6) via the generation of oxygen and alleviation of hypoxia in lipopolysaccharide (LPS)-activated macrophages (RAW 264.7), which produce excessive amounts of reactive oxygen species (ROS). GC-MnO NPs were synthesized by a simple reaction between GC and KMnO in water. The prepared GC-MnO NPs were spherical in shape, with a mean diameter of approximately 60 nm. The particles effectively generated oxygen via HO-induced degradation under hypoxic conditions, which led to an increase in the singlet oxygen levels upon laser irradiation. Furthermore, GC-MnO NPs significantly enhanced the light-triggered photodynamic effects of Ce6 on activated macrophages under hypoxic conditions, as shown by the increased levels of cell death and cell membrane damage in activated macrophages. Therefore, these results suggest that GC can be used as an alternative natural polymer for the synthesis of MnO NPs and that oxygen-generating GC-MnO NPs enhance the light-triggered photodynamic effects of Ce6 on activated macrophages by alleviating hypoxia.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.06.036DOI Listing
August 2021

Sutureless Transplantation of Amniotic Membrane Using a Visible Light-Curable Protein Bioadhesive for Ocular Surface Reconstruction.

Adv Healthc Mater 2021 07 29;10(13):e2100100. Epub 2021 May 29.

Department of Chemical Engineering, Pohang University of Science and Technology, Pohang, 37673, Republic of Korea.

The conjunctiva is a thin mucous membrane of the eye. Pterygium, a commonly appearing disease on the ocular surface, requires surgery to excise the conjunctiva to prevent visual deterioration. Recently, transplantation of the amniotic membrane (AM), which is the innermost membrane of the placenta, has been highlighted as an efficient method to cure conjunctiva defects because of its advantages of no side effects compared to mitomycin C treatment and not leaving additional scars on donor site compared to conjunctival autografting. However, to minimize additional damage to the ocular surface by suturing, AM transplantation (AMT) needs to be simplified by using a less invasive, time-saving method. In this work, a visible light-curable protein bioadhesive (named FixLight) for efficient sutureless AMT is applied. FixLight, which is based on bioengineered mussel adhesive protein (MAP), is easily applied between damaged ocular surfaces and transplanted AM, and rapidly cured by harmless blue light activation. Through in vivo evaluation using a rabbit model, the authors demonstrated that FixLight enabled facile, fast, and strong attachment of AM on sclera and promoted ocular surface reconstruction with good biocompatibility. Thus, FixLight can be successfully used as a promising clinical bioadhesive in opthalmological surgeries that require sutureless and rapid operation.
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http://dx.doi.org/10.1002/adhm.202100100DOI Listing
July 2021

Optimization of electrocoagulation process parameters for enhancing phosphate removal in a biofilm-electrocoagulation system.

Water Sci Technol 2021 May;83(10):2560-2574

Tianjin Water Engineering Co., Ltd, Tianjin, 300222, China.

This study aimed to enhance the removal of phosphate in synthetic rural sewage by using a continuous electrocoagulation (EC) combined with biofilm process in an integrated system. Characteristic indexes of biofilm process effluent covering pH, dissolved oxygen (DO), suspended solids (SS), chemical oxygen demand (COD) and phosphate maintained a narrow fluctuation range and tended not readily to influence the phosphate removal of subsequent electrocoagulation. Three parameters including inter-electrode distance, current intensity and reaction time were selected to investigate the performance of enhancing phosphate removal. On the strength of single-factor tests, the Box-Behnken design (BBD) coupled with response surface methodology (RSM) was applied to investigate the individual and mutual interaction impacts of the major operating parameters and to optimize conditions. The optimum conditions were found to be inter-electrode distance of 1.8 cm, current density of 2.1 mA/cm and EC reaction time of 34 min, and phosphate removal efficiency of 90.24% was achieved along with less than 1 mg/L in case of periodic polarity switching mode, which raised removal efficiency by 10.10% and reduced operating cost by 0.13 CNY/g PO compared to non-switching mode. The combination of biofilm processing and electrocoagulation treatment was proven to be a valid and feasible method for enhancing phosphate removal.
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http://dx.doi.org/10.2166/wst.2021.132DOI Listing
May 2021

DNA origami single crystals with Wulff shapes.

Nat Commun 2021 05 21;12(1):3011. Epub 2021 May 21.

National Laboratory of Solid State Microstructures, College of Engineering and Applied Sciences, State Key Laboratory of Analytical Chemistry for Life Science, Jiangsu Key Laboratory of Artificial Functional Materials, Nanjing University, Nanjing, China.

DNA origami technology has proven to be an excellent tool for precisely manipulating molecules and colloidal elements in a three-dimensional manner. However, fabrication of single crystals with well-defined facets from highly programmable, complex DNA origami units is a great challenge. Here, we report the successful fabrication of DNA origami single crystals with Wulff shapes and high yield. By regulating the symmetries and binding modes of the DNA origami building blocks, the crystalline shapes can be designed and well-controlled. The single crystals are then used to induce precise growth of an ultrathin layer of silica on the edges, resulting in mechanically reinforced silica-DNA hybrid structures that preserve the details of the single crystals without distortion. The silica-infused microcrystals can be directly observed in the dry state, which allows meticulous analysis of the crystal facets and tomographic 3D reconstruction of the single crystals by high-resolution electron microscopy.
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http://dx.doi.org/10.1038/s41467-021-23332-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8140131PMC
May 2021

Quantitative analysis of contrast-enhanced ultrasonography following living donor liver transplantation: early diagnosis of middle hepatic venous occlusion.

Med Ultrason 2021 May 20. Epub 2021 May 20.

Department of Surgery, Samsung Medical Center.

Aim: This study aimed to evaluate whether a quantitative contrast-enhanced ultrasonography (CEUS) study is feasible to diagnose middle hepatic venous occlusion after living donor liver transplantation (LDLT).

Materials And Methods: From December 2018 to July 2019, the CEUS study on the first postoperative day had been conducted in patients who underwent LDLT. 46 patients were finally included in the study. To obtain CEUS parameters from time-intensity curves (TICs) on the hepaticparenchyma, the two regions of interests (ROIs) were located in the right hepatic vein (RHV) territory and middle hepatic vein (MHV) territory of the right hepatic graft. The measured CEUS parameters were wash-in slope (WIS), peak intensity (PI), time to peak (TTP), and area under the curve (AUC). The subjects were classified into the occlusion and non-occlusion groups. In each group, the parameters measured in the RHV and MHV territories were compared with paired-sample Student'st-tests.

Results: Hepatic venous occlusion was diagnosed in 25 patients (54%). The WIS, TTP, and AUC of the MHV territory (2.95 dB/sec; 22.39 sec; 204.27 dB·sec, respectively) were significantly different from those of the RHV territory (2.16 dB/sec; 25.81 sec; 165.66 dB·sec; all p<0.05). There were no statistically significant differences in PI between the MHV and RHV territories (19.08 dB vs. 18.27 dB, respectively; p=0.259). In the non-occlusion group, there was no parameter which was significantly different between MHV and RHV territories (p>0.05).

Conclusion: The parametric analysis of CEUS can help diagnose middle hepatic venous occlusion after LDLT.
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http://dx.doi.org/10.11152/mu-2906DOI Listing
May 2021

Clinical significance of heart rate variability for the monitoring of cardiac autonomic neuropathy in end-stage renal disease patients.

Nutr Metab Cardiovasc Dis 2021 06 26;31(7):2089-2098. Epub 2021 Mar 26.

Division of Nephrology, Department of Internal Medicine, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, South Korea. Electronic address:

Background And Aims: The aim of this study is to determine whether the measurement of continuous heart rate variability (HRV) is useful in the evaluation of cardiac autonomic neuropathy (CAN) in end-stage renal disease (ESRD) patients.

Methods And Results: This cross-sectional study was performed at Seoul St. Mary's hospital between June 2017 and February 2018. Seventy-seven ESRD patients, and 29 healthy controls (HCs) were asked to wear a continuous ambulatory HRV monitor for 24 h. General cardiac function was evaluated using transthoracic echocardiogram (TTE), pulse wave velocity (PWV), coronary calcium scoring (CCS), and 24-h ambulatory blood pressure monitoring (ABPM). HRV parameters of ESRD patients and HCs, and the correlation of HRV parameters with cardiovascular screening methods were observed. All HRV parameters were significantly decreased in ESRD patients compared to HCs (P < 0.001). In the correlation analysis between TTE results and HRV parameters, 24-h standard deviation of all N-N intervals (24SDNN), 24-h standard deviation of sequential 5-min N-N interval means (24DANN) and Low Frequency Power/High Frequency Power (LF/HF) ratio showed negative correlations with E/e', LAVI and TR velocity which are representative indices for the diastolic function of the heart (P < 0.05). HRV parameters showed negative correlations with baPWV, CCS, and 24-h ABPM results as well (P < 0.05). Hemoglobin and serum albumin showed positive correlations with HRV parameters, and glucose, BUN, creatinine, and iPTH levels showed negative correlations (P < 0.05).

Conclusion: Continuous HRV monitoring may be a useful tool for the evaluation of CAN in ESRD.
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http://dx.doi.org/10.1016/j.numecd.2021.03.016DOI Listing
June 2021

Sarcomatoid carcinoma of the pancreas - multimodality imaging findings with serial imaging follow-up: A case report and review of literature.

World J Clin Cases 2021 May;9(13):3102-3113

Department of Pathology, Cancer Research Institute, College of Medicine, Chungnam National University, Daejeon 35015, South Korea.

Background: Sarcomatoid carcinoma of the pancreas is extremely rare and has an extremely poor prognosis. Although a few cases of sarcomatoid carcinoma of pancreas have been reported, most are focused on a histopathological review. To the best of our knowledge, there are no reports documenting multimodality imaging characteristics and chronological changes with emphasis on radiologic features.

Case Summary: A 64-year-old woman was admitted to Chungnam National University Hospital with acute appendicitis. Contrast-enhanced computed tomography of the abdomen revealed a 2.6 cm × 2.8 cm multilobular cystic mass in the pancreatic tail. The pancreatic lesion showed suspected mural nodules and thin septa. Hence, mucinous cystic neoplasm of pancreas was considered. After 7 mo, the patient was readmitted for repeated epigastric abdominal pain and nausea. Follow-up contrast-enhanced computed tomography of the abdomen and magnetic resonance imaging revealed a marked enlargement (5.4 cm × 4 cm), with a predominant internal solid component. The mass showed low signal intensity on a T1-weighted image and heterogeneously intermediate high signal intensity on a T2-weighted image. It showed diffusion restriction and peripheral rim enhancement on an arterial phase image, and progressive enhancement on portal venous and delayed phase images. Distal pancreatectomy was performed. Based on the morphology and immunohistochemical staining of the specimen, pancreatic sarcomatoid carcinoma was diagnosed.

Conclusion: We present the computed tomography, magnetic resonance imaging, and positron emission tomography computed tomography findings, pathologic features, and chronological changes for preoperative diagnosis.
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http://dx.doi.org/10.12998/wjcc.v9.i13.3102DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8080743PMC
May 2021

Recyclable and Magnetically Functionalized Metal-Organic Framework Catalyst: IL/[email protected] for the Cycloaddition Reaction of CO with Epoxides.

ACS Appl Mater Interfaces 2021 May 9;13(19):22836-22844. Epub 2021 May 9.

School of Chemistry, Faculty of Chemical, Environmental and Biological Science and Technology, Dalian University of Technology, Dalian 116023, P.R. China.

A recyclable and magnetic nanocomposite catalyst (IL/[email protected]) was synthesized via grafting ionic liquid (IL) [AEMIm]BF into magnetically functionalized metal-organic framework [email protected] in a water-ethanol media. The properties of IL/[email protected]UST-1 were fully characterized by powder X-ray diffraction, electron microscopy, Fourier-transform infrared spectroscopy, nitrogen adsorption-desorption, density-functional theory, and a magnetic property measurement system. IL/[email protected] showed high activity in the solvent-free cycloaddition of CO with epoxides under mild conditions. Furthermore, the catalyst can be easily separated from the reaction mixture, and the recycled catalyst maintained high performance for several cycles. The synergistic effect of the Lewis acid and base sites in IL/[email protected] contributes to its greater reactivity than individual IL or HKUST-1.
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http://dx.doi.org/10.1021/acsami.1c03345DOI Listing
May 2021
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