Publications by authors named "Min Cui"

238 Publications

Temporary vaccination clinic for COVID-19 in Zhuhai, China.

Hum Vaccin Immunother 2021 Jun 11:1-3. Epub 2021 Jun 11.

Deputy Secretary of Party Committee & Vice President, Zhuhai People's Hospital (Zhuhai Hospital Affiliated with Jinan University), Zhuhai, China.

Vaccines are urgently needed to control the COVID-19 pandemic. To gradually increase the vaccination rate among residents, temporary vaccination clinic for COVID-19 plays an important role. It should be located in an area with convenient transportation and concentrated population. Functional zones including waiting and inquiry, registration and notification, injection, observation and emergency room should be established. All vaccine recipients' information should be uploaded to the national immunization information system. Medical staff at the temporary vaccination clinic should be professionally trained. A cautious disinfection and wiping are essential for the temporary vaccination clinic.
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http://dx.doi.org/10.1080/21645515.2021.1933688DOI Listing
June 2021

Variations and characteristics of carbonaceous substances emitted from a heavy fuel oil ship engine under different operating loads.

Environ Pollut 2021 May 22;284:117388. Epub 2021 May 22.

Key Lab of Geographic Information Science of the Ministry of Education, School of Geographic Sciences, East China Normal University, Shanghai, 210062, China; Institute of Eco-Chongming, 3663 North Zhongshan Road, Shanghai, 200062, China.

Heavy fuel oil (HFO) accounts for approximately 80% of the fuel consumption of ocean-going ships in the world. Multiple toxic species are found in HFO exhaust, however, carbonaceous substances emitted from low-speed marine engine exhaust at different operating loads have not been thoroughly addressed. Therefore, a bench test for a low-speed marine engine with HFO fuel under different operating modes was carried out in this study. Emission factors and characteristics of CO, CO, organic carbon (OC), elemental carbon (EC), as well as OC and EC fragments, organic matters of n-alkanes and polycyclic aromatic hydrocarbons (PAHs) are given and discussed. Combined with the correlation analysis results among the measured species and engine technical parameters, the formation processes and influence factors of carbonaceous components are also inferred in this study. Besides, together with OC to EC ratio, n-alkanes to PAHs ratio, etc., EC1 to soot-EC ratio in PM can be considered as tracer characteristic of high-sulfur-content HFO ship distinguished from diesel fuel ships. Profiles of n-alkanes and PAHs in PM can be used to distinguish shipping emission source from other combustion sources. Moreover, characteristics of carbonaceous components in size-segregated particles are also discussed, including OC, EC, OC and EC fragments, as well as organic matters. Results show that most of the particle mass, OC, EC, and organic matters are concentrated in fine particles with size of less than 1.1 μm, indicating the significance of ultrafine particles. Formation processes of OC and EC fragments, EC1 and soot-EC are also deduced and proved combined with the characteristics of OC and EC fragments, organic matters, and especially PAHs. Besides, the large variations of OC to EC ratios and speciated profiles of n-alkanes and PAHs in different particle size bins indicate that particle size should be considered when they are used as characteristic tracer in source apportionment studies.
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http://dx.doi.org/10.1016/j.envpol.2021.117388DOI Listing
May 2021

Analysis of the coverage of inactivated enterovirus 71 (EV71) vaccine and adverse events following immunization with the EV71 vaccine among children from 2016 to 2019 in Guangzhou.

Expert Rev Vaccines 2021 Jun 7:1-12. Epub 2021 Jun 7.

Ministry of immunization, Guangzhou Center For Disease Control And Prevention.

: Since 2016, China has approved the use of inactivated enterovirus 71 (EV71) vaccines produced by three manufacturers. The coverage and safety of different EV71 vaccines need to be evaluated.: The EV71 vaccination and AEFI data were collected from the Guangzhou Children's Vaccination Information Report Management System and the China AEFI Monitoring Information Management System, and the EV71 vaccine coverage rate and the AEFI incidence rate were analyzed.: From 2016 to 2019, the number of people who should have been vaccinated in Guangzhou was 2,781,618, and the coverage rates for doses 1 and 2 were 24.71% and 19.44%, respectively. The inoculation rates of vaccines from manufacturers A and B were between 3.03 and 10.46%. The reported incidence of AEFIs was 11.97 per 100,000 (147 cases), with fever (106 cases, 8.63 per 100,000) and allergic rash (59 cases, 4.80 per 100,000) being the most common reactions. There were no differences in the AEFI responses to the EV71 vaccines from the three manufacturers.: The EV71 vaccines from the three manufacturers have good safety, but the EV71 vaccine coverage rate is low. It is recommended that vaccine publicity be strengthened and that the vaccine coverage rate in children be increased.
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http://dx.doi.org/10.1080/14760584.2021.1933451DOI Listing
June 2021

Neuroprotective effect of miR-212-5p on isoflurane-induced cognitive dysfunction by inhibiting neuroinflammation.

Toxicol Mech Methods 2021 May 24:1-6. Epub 2021 May 24.

Department of Anesthesiology, Zaozhuang Municipal Hospital, Zaozhuang, Shandong, P. R. China.

Background: Isoflurane is a commonly used inhalation anesthetic in the clinic, which can induce cognitive dysfunction and neuroinflammation. miR-212-5p has been demonstrated to be involved in the neuronal system and play vital roles in memory formation. Its function in the learning and memory impairment and neuroinflammation induced by isoflurane was investigated in this study.

Methods: Cognitive dysfunction rat models were established by 3% isoflurane inhalation. The neurological function was evaluated by the modified Neurological Severity Scale. The learning and memory ability of rats was assessed by the Morris water maze test. The expression level of miR-212-5p was analyzed by RT-qPCR, and the protein levels of proinflammatory cytokines were detected by ELISA.

Results: Isoflurane induced cognitive dysfunction in rats with the neurological scores and the escape latency increased, and time spent in the target quadrant decreased. The protein levels of IL-1β, IL-6, and TNF-α were increased in isoflurane treated rats. miR-212-5p was downregulated in cognitive impairment rats. The upregulation of miR-212-5p by the agomir injection decreased the neurological scores of rats and increased the learning and memory ability of impaired rats. Moreover, the neuroinflammation was inhibited by the overexpression of miR-212-5p.

Conclusion: miR-212-5p showed a neuroprotective effect in isoflurane-induced cognitive dysfunction rats by inhibiting neuroinflammation.
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http://dx.doi.org/10.1080/15376516.2021.1919948DOI Listing
May 2021

Variability in real-world emissions and fuel consumption by diesel construction vehicles and policy implications.

Sci Total Environ 2021 Sep 22;786:147256. Epub 2021 Apr 22.

Research Center for Eco-Environmental Engineering, Dongguan University of Technology, Dongguan 523830, China. Electronic address:

Strategically reducing the emission of non-road mobile source especially diesel construction vehicle (DCV) has a large potential in improving air quality and has attracted much scientific and public attention in recent years around the world. In this study, we explored real-world fuel consumption rate and gaseous emissions factors for multiple pollutants of three typical DCVs in China. The sampling campaign considered the operation mode, cumulative operation hour, emission standard stage and engine power. Results show that the accumulated fuel consumption per hour of vehicle weight for working, load-free moving and idling modes was 0.3, 0.2 and 0.1 kg/h·tons, respectively. The fuel-based NOx emission factor exhibited a bimodal distribution at 27 and 41 g/kg. The fuel-based emission factors for volatile organic compounds (VOCs) were in the range of 0.8 to 2.6 g/kg, where alkene and alkane were the dominant components (>80%), i.e., ethylene, acetylene, propylene, and isobutane. We observed that the ratio of toluene and benzene concentration (T/B) (1.4 ± 1.3) differed from other key emission sources and may be used as the specific indicator of DCV emission exhaust. Our estimates suggest that in 2017 the fuel consumption and NOx emissions of DCV emission accounted for 22-28% of non-road mobile sources in China; NO emissions were 2.7 times higher than those in 2006, and it is forecasted that NOx emissions would reduce by 23% between 2017 and 2025 with the implementation of stage IV and the strict supervision policy. The comprehensive dataset on DCV emissions will either guide the government to establish precise and effective policies to regulate the non-road mobile source or significantly improve our understanding of source apportionment of atmospheric NOx and VOCs, both of which are key precursors of haze and ozone pollution.
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http://dx.doi.org/10.1016/j.scitotenv.2021.147256DOI Listing
September 2021

Near-infrared intraoperative imaging of pelvic autonomic nerves: a pilot study.

Surg Endosc 2021 Apr 28. Epub 2021 Apr 28.

Zhuhai People's Hospital (Zhuhai Hospital Affiliated with Jinan University), Zhuhai, Guangdong Province, 519000, China.

Background: The pelvic autonomic nerves control and regulate anorectal and urogenital function. The dysfunction of pelvic autonomic nerves lead to disorders of anorectum, bladder and male sex organs. Thus the intraoperative identification of pelvic autonomic nerves could be crucial in complications prevention and diseases treatment. Our clinical trial aims at estimating the effectiveness and validity of intraoperative indocyanine green fluorescence imaging in pelvic autonomic nerves identification.

Methods: Intraoperative fluorescence imaging using indocyanine green was performed in ten patients and the feasibility was determined. From February 2019 to June 2019, the seven patients undergoing laparoscopic colorectal resection was administrated 4.5 mg/Kg indocyanine green 24 h before surgery. The near-infrared fluorescence imaging was conducted during surgery. A novel white light and near-infrared dual-channel laparoscopic equipment was applied. For each patient, signal-background ratio values for pelvic autonomic nerves were recorded and analyzed.

Results: We confirmed the dose and timing of indocyanine green administration was 4.5 mg/Kg and 24 h before surgery. Using the dual laparoscopic equipment, we could observe the splanchnic plexus, inferior mesenteric artery plexus, and sacral plexus successfully with a high signal background ratio value of 3.18 (standard deviation: 0.48).

Conclusion: This pilot trial shows feasibility of intraoperative indocyanine green fluorescence imaging in pelvic autonomic nerves observation. It demonstrates that nerves can be visualized using alternative imaging techniques but it is not ready yet for prime time. This technique might aid observation with white light alone. Registration number: ChiCTR1900025336.
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http://dx.doi.org/10.1007/s00464-021-08512-zDOI Listing
April 2021

A novel oral rabies vaccine enhances the immunogenicity through increasing dendritic cells activation and germinal center formation by expressing U-OMP19 in a mouse model.

Emerg Microbes Infect 2021 Dec;10(1):913-928

Key Laboratory of Preventive Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, People's Republic of China.

Rabies remains a public health threat in most parts of the world. Dogs, especially stray dogs, are the main sources of rabies transmission in developing countries, while wild animals are primarily responsible for the spread of rabies in developed countries and play an emerging role in rabies transmission in developing countries. Oral vaccination is the most practical method for rabies control in these animals, and the greatest challenge for oral vaccination is the hostile environment and large quantity of proteases in the gastrointestinal tract. In the present study, a promising adjuvant with potential protease inhibitory activity, unlipidated outer membrane protein 19 (U-OMP19), was inserted into the genome of the recombinant rabies virus (rRABV) strain LBNSE, designated LBNSE-U-OMP19, and the immunogenicity of LBNSE-U-OMP19 was investigated. LBNSE-U-OMP19 could potentially protect viral glycoprotein from digestion by gastrointestinal fluids . The expression of U-OMP19 attenuated viral pathogenicity by restricting viral replication in the central nervous system (CNS) and repressing the production of inflammatory chemokines and cytokines. After oral vaccination, LBNSE-U-OMP19 recruited dendritic cells (DCs), follicular helper T (T) cells and germinal center (GC) B cells, promoted the formation of GCs, and increased the population of plasma cells in immunized mice, resulting in higher levels of RABV-neutralizing antibodies and better protection in mice immunized with LBNSE-U-OMP19 than in those immunized with the parent virus LBNSE. Together, our data suggest that LBNSE-U-OMP19 is a promising candidate for oral rabies vaccines.
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http://dx.doi.org/10.1080/22221751.2021.1923341DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8143638PMC
December 2021

A Propensity Score Matching Study Between Microwave Ablation and Radiofrequency Ablation in Terms of Safety and Efficacy for Benign Thyroid Nodules Treatment.

Front Endocrinol (Lausanne) 2021 9;12:584972. Epub 2021 Mar 9.

Zhuhai People's Hospital (Zhuhai Hospital Affiliated with Jinan University), Zhuhai, China.

Background: Large benign thyroid nodules often lead to cosmetic problems and compression on trachea. Thermal ablation is an effective method for benign thyroid nodules treatment. Among all the thermal ablation techniques, microwave and radiofrequency are frequently used energy sources. However, treatment outcomes of the two ablation types have not been compared in detail. Therefore, we conducted this study aiming for comparing the safety and efficacy of the two ablation techniques in benign thyroid nodules treatment.

Methods: Information was retrospectively collected from patients with benign thyroid nodules, who received radiofrequency ablation or microwave ablation between January 1, 2018, and December 31, 2019, in a main hospital in South China. Patients were divided into microwave ablation group and radiofrequency ablation group according to the techniques applied. A propensity score matching was performed to balance the baseline indexes between the two groups. We also recorded and analyzed the operative variables including operative duration, intraoperative blood loss, hospitalization time, and overall costs. Postoperative quality of life, volume reduction rates, and complication rates were routinely evaluated during the follow-up by asking patients to fulfil questionnaires at the 1, 3, 6, 12, and 18 postoperative month.

Results: A total of 943 patients receiving microwave ablation or radiofrequency ablation in the years of 2018 and 2019 met our inclusion criteria. After 1:1 propensity score matching, 289 pairs of patients were matched. There was no significant difference between the two groups in operative duration, intraoperative blood loss, hospitalization time, overall cost, quality of life scores, complication rates or volume reduction rates.

Conclusion: There was no significant difference between microwave and radiofrequency ablation in terms of safety and efficacy. Both of the two techniques` are ideal therapeutic methods in benign thyroid nodules treatment.

Registration Number: ChiCTR2000034764.
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http://dx.doi.org/10.3389/fendo.2021.584972DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7985541PMC
March 2021

PTP-MEG2 regulates quantal size and fusion pore opening through two distinct structural bases and substrates.

EMBO Rep 2021 05 25;22(5):e52141. Epub 2021 Mar 25.

Key Laboratory Experimental Teratology of the Ministry of Education and Department of Physiology, Shandong University School of Medicine, Jinan, China.

Tyrosine phosphorylation of secretion machinery proteins is a crucial regulatory mechanism for exocytosis. However, the participation of protein tyrosine phosphatases (PTPs) in different exocytosis stages has not been defined. Here we demonstrate that PTP-MEG2 controls multiple steps of catecholamine secretion. Biochemical and crystallographic analyses reveal key residues that govern the interaction between PTP-MEG2 and its substrate, a peptide containing the phosphorylated NSF-pY site, specify PTP-MEG2 substrate selectivity, and modulate the fusion of catecholamine-containing vesicles. Unexpectedly, delineation of PTP-MEG2 mutants along with the NSF binding interface reveals that PTP-MEG2 controls the fusion pore opening through NSF independent mechanisms. Utilizing bioinformatics search and biochemical and electrochemical screening approaches, we uncover that PTP-MEG2 regulates the opening and extension of the fusion pore by dephosphorylating the DYNAMIN2-pY and MUNC18-1-pY sites. Further structural and biochemical analyses confirmed the interaction of PTP-MEG2 with MUNC18-1-pY or DYNAMIN2-pY through a distinct structural basis compared with that of the NSF-pY site. Our studies thus provide mechanistic insights in complex exocytosis processes.
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http://dx.doi.org/10.15252/embr.202052141DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8097337PMC
May 2021

COVID-19 Emergencies in East Asian Countries: Experiences and Challenges.

Asia Pac J Public Health 2021 Mar 18:10105395211001658. Epub 2021 Mar 18.

Zhuhai People's Hospital (Zhuhai Hospital Affiliated with Jinan University), Zhuhai, Guangdong Province, China.

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http://dx.doi.org/10.1177/10105395211001658DOI Listing
March 2021

A systematic review on the application of the hybrid operating room in surgery: experiences and challenges.

Updates Surg 2021 Mar 11. Epub 2021 Mar 11.

China's Communist Party Committee, Zhuhai People's Hospital (Zhuhai Hospital Affiliated with Jinan University), No. 79 of Kangning Road, Xiangzhou District, Zhuhai City, 519000, Guangdong Province, China.

The hybrid operating room has been widely applied in different surgery sub-specialties. We aim to identify the advantages of hybrid operating rooms by focusing on intraoperative imaging and explore what to do for further improving its application. We searched related literature in websites including Pubmed, MEDLINE, Web of science, using the keywords ("hybrid operating room" or "integrated operating room" or "multifunctional operating room") and ("surgery" or "technique" or "intervention" or "radiology"). All the searched papers were screened and underwent quality evaluation. A total of 30 literature was eventually identified after full-text screening. These articles covered 10 countries and presented data for 15,558 individuals. The median sample size was 536 (range 8-12,804). Application of the hybrid operating room in general surgery, neurosurgery, thoracic surgery, urology, gynaecologic and obstetrics surgery, cardiovascular surgery, was summarized. Four different operative indicators were applied (operative duration, mortality rate, operation success rate and complication rate). A hybrid OR could significantly increase the operation success rate and reduce operative duration, mortality rates, and complication rates. Further efforts could be made to reduce radiation exposure in the hybrid operating room and increase its cost-effectiveness ratio.
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http://dx.doi.org/10.1007/s13304-021-00989-6DOI Listing
March 2021

Tannins extract from Galla Chinensis can protect mice from infection by Enterotoxigenic Escherichia coli O101.

BMC Complement Med Ther 2021 Mar 6;21(1):84. Epub 2021 Mar 6.

Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China.

Background: Enterotoxigenic Escherichia coli (ETEC) is classically associated with acute secretory diarrhea, which induces 2 million people death in developing countries over a year, predominantly children in the first years of life. Previously, tannins (47.75%) were extracted from Galla Chinensis and prepared as Galla Chinensis oral solution (GOS) which showed significant antidiarrheal activity in a castor oil-induced diarrhea in mice. Whether the tannins extract were also effective in treatment of ETEC-induced diarrhea was determined in this study.

Methods: Mice were randomly divided into 6 groups (n = 22). The mice in the normal and untreated groups were given normal saline. Three GOS-treated groups were received different concentrations of GOS (5, 10 and 15%, respectively) at a dose of 10 mL/kg. Mice in the positive control group were fed with loperamide (10 mg/kg). The treatment with GOS started 3 days before infection with ETEC and continued for 4 consecutive days after infection. On day 3, mice were all infected with one dose of LD of ETEC, except those in the normal group. Survival of mice was observed daily and recorded throughout the study. On days 4 and 7, samples were collected from 6 mice in each group.

Results: GOS could increase the survival rate up to 75%, while in the untreated group it is 43.75%. The body weights of mice treated with 15% GOS were significantly increased on day 7 in comparison with the untreated group and the normal group. GOS-treatment recovered the small intestine coefficient enhanced by ETEC-infection. The diarrhea index of mice treated with GOS was significantly decreased. GOS increased the levels of IgG and sIgA in the terminal ileum and decreased the levels of pro-inflammatory cytokines (IFN-γ, TNF-α, IL-1β, IL-6 and IL-8) in serum. GOS could increase the amount of intestinal probiotics, Lactobacilli and Bifidobacteria. GOS could alleviate colon lesions induced by ETEC-infection. GOS showed higher potency than loperamide.

Conclusions: GOS could be a promising drug candidate for treating ETEC infections.
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http://dx.doi.org/10.1186/s12906-021-03261-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7937208PMC
March 2021

Study protocol: A cross-sectional study on instrumental support for transitional care among older adults with chronic diseases.

Nurs Open 2021 07 6;8(4):1990-1997. Epub 2021 Mar 6.

School of Medical, Nantong University, Nantong, China.

Aims: In this paper, we present a study aiming to develop a questionnaire on instrumental support for transitional care as a tool for investigating services, staff, equipment and supplies, and funds of transitional care and conduct a cross-sectional study on the current instrumental support for transitional care in older adults with chronic diseases.

Design: A cross-sectional study combining instrumental support with transitional care through a mixed-method approach.

Methods: Data are collected through two sources: distribution of the questionnaire to older adults with chronic diseases and interviews with experts from different specialties such as nursing, clinical medicine, geriatrics, sociology and government.

Results: The developed questionnaire and expert interviews will be used to investigate the current instrumental support for transitional care among older adults with chronic diseases in China. These findings can help leaders identify areas for improvement in transitional care and contribute to the long-term positive development of transitional care.
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http://dx.doi.org/10.1002/nop2.837DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8186703PMC
July 2021

Association between risk factors and retinal nerve fiber layer loss in early stages of diabetic retinopathy.

Int J Ophthalmol 2021 18;14(2):255-262. Epub 2021 Feb 18.

Department of Human Anatomy, Nanjing Medical University, Nanjing 211166, Jiangsu Province, China.

Aim: To investigate the changes of retinal nerve fiber layer (RNFL) among normal individuals, diabetic patients without diabetic retinopathy (NDR) and non-proliferative diabetic retinopathy (NPDR), and explore the possible risk factors of early diabetic retinopathy (DR).

Methods: In this cross-sectional study, 107 participants were divided in three groups. Totally 31 normal individuals (control group), 40 diabetic patients without DR (NDR group) and 36 patients with NPDR (NPDR group) were included. Optical coherence tomography (OCT) was used to detect RNFL thickness and other optic disc parameters among different groups. The potential association between RNFL loss and systemic risk factors were assessed for DR, including diabetes duration, body mass index (BMI), hemoglobin A1c (HbA1c), serum lipids, and blood pressure.

Results: The average and each quadrant RNFL thickness were thinner in NPDR group compared to control group of the right (=0.00, =0.01, =0.01, =0.02, =0.04) and left eyes (=0.00, =0.00, =0.00, =0.03, =0.04). The average, superior and inferior RNFL thickness were thinner in NDR group compared to the NPDR group of the right (=0.00, =0.02, =0.03) and left eyes (=0.00, =0.00, =0.01). Diabetic duration was negatively correlated with the superior, inferior, and average RNFL thickness of the right (=-0.385, =0.001; =-0.366, =0.001; =-0.503, =0.000) and left eyes (=-0.271, =0.018; =0.278, =0.015; =-0.260, =0.023). HbA1c was negatively correlated with the superior, inferior, and average RNFL thickness of the right (=-0.316 =0.005; =-0.414, =0.000; =-0.418, =0.000) and left eyes (=-0.367, =0.001; =-0.250, =0.030; =-0.393, =0.000). Systolic pressure was negatively correlated with the inferior and average RNFL thickness of the right eye (=-0.402, =0.000; =-0.371, =0.001) and was negatively correlated with the superior and average RNFL thickness of the left eye (=-0.264, =0.021; =-0.233, =0.043).

Conclusion: RNFL loss, especially in the superior and inferior quadrants, may be the earliest structural change of the retina in diabetic patients, and is also associated with diabetic duration, HbA1c, and systolic pressure.
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http://dx.doi.org/10.18240/ijo.2021.02.12DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7840372PMC
February 2021

Sterol metabolism and protein metabolism are differentially correlated with sarcopenia in Asian Chinese men and women.

Cell Prolif 2021 Apr 20;54(4):e12989. Epub 2021 Feb 20.

Department of Health Care and Department of Health Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC), Beijing, China.

Objectives: Our aim was to investigate the prevalence and predictive variables of sarcopenia.

Methods: We recruited participants from the Peking Union Medical College Hospital Multicenter Prospective Longitudinal Sarcopenia Study (PPLSS). Muscle mass was quantified using bioimpedance, and muscle function was quantified using grip strength and gait speed. Logistic regression revealed the relationships between sarcopenia and nutritional, lifestyle, disease, psychosocial and physical variables.

Results: The prevalence of sarcopenia and sarcopenic obesity was 9.2%-16.2% and 0.26%-9.1%, respectively. Old age, single status, undernourishment, higher income, smoking, low physical activity, poor appetite and low protein diets were significantly associated with sarcopenia. Multiple logistic regression analysis showed that age was a risk factor for all stages of sarcopenia, and participants above 80 years were greater than fivefold more susceptible to sarcopenia, while lower physical activity was an independent risk factor. The optimal cut-off value for age was 71 years, which departs from the commonly accepted cut-off of 60 years. Female participants were greater than twofold less susceptible to sarcopenia than male participants. The sterol derivative 25-hydroxyvitamin D was associated with fourfold lower odds of sarcopenia in male participants. Several protein intake variables were also correlated with sarcopenia. Based on these parameters, we defined a highly predictive index for sarcopenia.

Conclusions: Our findings support a predictive index of sarcopenia, which agglomerates the complex influences that sterol metabolism and nutrition exert on male vs female participants.
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http://dx.doi.org/10.1111/cpr.12989DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8016649PMC
April 2021

High-time-resolution PM source apportionment based on multi-model with organic tracers in Beijing during haze episodes.

Sci Total Environ 2021 Jun 23;772:144766. Epub 2021 Jan 23.

Chinese Research Academy of Environmental Sciences, Beijing 100012, PR China. Electronic address:

Fine particulate matter (PM) is a prominent atmospheric pollutant that poses serious adverse effects on air quality and human health. PM source apportionment based on receptor model suggests that Beijing is polluted by mixed emission sources, but the model is limited by a lack of organic tracers and an inability to distinguish between contributions from local and regional transport. In this study, positive matrix factorization (PMF) model with organic tracers was employed to analyze refined PM pollution sources at 1-h time resolution, and the contribution of regional transport was quantified using Particulate source apportionment technology (PSAT) in the Comprehensive Air Quality Model with Extensions (CAMx). The results identified nine source types using PMF model based on offline data for PM concentrations, organic carbon, elemental carbon, water-soluble ions, trace elements and organic species. Gasoline and diesel exhausts were distinguished by adding polycyclic aromatic hydrocarbons (PAHs), C19-C24 n-alkanes as key organic tracers. In addition, levoglucosan and hexadecanoic acid are important additions for identifying biomass burning and cooking, respectively. Furthermore, the contribution of specific sources and source regions, from the formation to dissipation of two typical haze episodes (EP1 and EP2) in Beijing, was quantitatively analyzed. EP1 was primarily caused by local emissions with an average contribution rate of 67.5%, characterized by secondary source, gasoline and diesel exhausts, as well as industrial source. EP2 was dominated by secondary source from regional transport contributing approximately 50%. Short-range transport from Baoding (9.1%) and Langfang (5.8%) in Hebei Province was the largest external contributor, and long-range transport contributed 20% of the PM concentration. This study suggests that combining receptor model-based source apportionment with air quality model has practical significance for understanding the causes of haze episodes, setting city-specific emission reduction measures and improving air quality in the Beijing-Tianjin-Hebei (BTH) region.
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http://dx.doi.org/10.1016/j.scitotenv.2020.144766DOI Listing
June 2021

Efficacy and safety of tigecycline for complicated urinary tract infection: a systematic review.

Transl Androl Urol 2021 Jan;10(1):292-299

Department of Pharmacy, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.

Background: Facing the global threat of emerging resistance to antibiotics, tigecycline, a novel glycylcycline antibiotic, is developed to against multidrug-resistant pathogens, but not recommended for the treatment of complicated urinary tract infection (cUTI). We performed a summary of the literatures to characterize and evaluate the efficacy and safety of tigecycline in patients with cUTI.

Methods: We searched PubMed, EMBASE, Cochrane and Clinical Trials using appropriate syntax to retrieve potential articles up to Jan 2020. General information, pathogen, medication regimen, comorbidities of patients from eligible literatures were recorded. Univariate logistic regression analysis was used to detect the potential factors associated with clinical cure.

Results: Nineteen articles comprising 31 cases were included. The subpopulation with transplantation (25.8% of the patients) was the most common comorbidity, and cUTIs were mainly caused by () (48.28%) in our research. Tigecycline 100 mg per day as monotherapy was most common. Clinical cure was reported as majority (77.4%), and microbiological eradication cases accounted for the most (65.2%) among the clinical cure cases. Univariate analysis showed that caused cUTI and tigecycline as a single treatment have significant meaning to clinical outcomes (P=0.044 and P=0.034, respectively).

Conclusions: Clinical and microbiological outcomes of tigecycline treatment revealed high rate of successful response. Tigecycline monotherapy may have a role in the treatment of cUTI except that caused by the pathogen . Further randomized controlled trials was still needed to evaluate tigecycline monotherapy for cUTI.
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http://dx.doi.org/10.21037/tau-20-959DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7844507PMC
January 2021

Research on Intra-Operative Indocyanine Green Angiography of the Parathyroid for Predicting Postoperative Hypoparathyroidism: A Noninferior Randomized Controlled Trial.

Authors:
Hao Jin Min Cui

Endocr Pract 2020 Dec;26(12):1469-1476

From The Second Department of General Surgery, Zhuhai People's Hospital (Zhuhai Hospital Affiliated with Jinan University), Zhuhai, China.. Electronic address:

Objective: A noninferiority randomized controlled trial was undertaken to clarify whether the postoperative measurements of serum calcium and parathyroid hormone and oral supplementation of calcium and calcitriol could be omitted if patients had at least one well-perfused parathyroid gland evaluated by intra-operative indocyanine green (ICG) angiography.

Methods: Patients with at least one parathyroid gland well-perfused by ICG angiography (ICG score >2) were randomized to the control group or test group. For the control group, oral calcium and calcitriol were systematically supplemented. For the test group, no oral calcium or calcitriol was supplemented to the patients. Levels of serum calcium and parathyroid hormone of patients on the first and 30th postoperative day were compared between the two groups.

Results: Among all 68 selected patients, 56 patients had at least one well-perfused parathyroid gland evaluated by intra-operative ICG angiography. The 56 patients were randomized to the control group or test group. There were no statistically significant differences in the levels of serum calcium and parathyroid hormone between test group and control group on the first or 30th postoperative day.

Conclusion: The postoperative measurements of serum calcium and parathyroid hormone and oral supplementation of calcium and calcitriol were evaluated as redundant, if patients had at least one well-perfused parathyroid gland evaluated by intra-operative ICG angiography.

Abbreviations: ICG = indocyanine green; NIR = near infrared; POD = postoperative day; PTH = parathyroid hormone; SBR = signal background ratio.
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http://dx.doi.org/10.4158/EP-2020-0340DOI Listing
December 2020

HIF1A Alleviates compression-induced apoptosis of nucleus pulposus derived stem cells via upregulating autophagy.

Autophagy 2021 Jan 18:1-23. Epub 2021 Jan 18.

Department of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Intervertebral disc degeneration (IDD) is the primary pathological mechanism that underlies low back pain. Overloading-induced cell death, especially endogenous stem cell death, is the leading factor that undermines intrinsic repair and aggravates IDD. Previous research has separately studied the effect of oxygen concentration and mechanical loading in IDD. However, how these two factors synergistically influence endogenous repair remains unclear. Therefore, we established and models to study the mechanisms by which hypoxia interacted with overloading-induced cell death of the nucleus pulposus derived stem cells (NPSCs). We found the content of HIF1A (hypoxia inducible factor 1 subunit alpha) and the number of NPSCs decreased with disc degeneration in both rats and human discs. Hence, we isolated this subpopulation from rat discs and treated them simultaneously with hypoxia and excessive mechanical stress. Our results demonstrated that hypoxia exerted protective effect on NPSCs under compression, partially through elevating macroautophagy/autophagy. Proteomics and knockdown experiments further revealed HIF1A-BNIP3-ATG7 axis mediated the increase in autophagy flux, in which HMOX1 and SLC2A1 were also involved. Moreover, HIF1A-overexpressing NPSCs exhibited stronger resistance to over-loading induced apoptosis . They also showed higher survival rates, along with elevated autophagy after being intra-disc transplanted into over-loaded discs. Jointly, both and experiments proved the anti-apoptotic effect of HIF1A on NPSCs under the excessive mechanical loading, suggesting that restoring hypoxia and manipulating autophagy is crucial to maintain the intrinsic repair and to retard disc degeneration. 3-MA: 3-methyladenine; ACAN: aggrecan; ATG7: autophagy related 7; BafA1: bafilomycin A; BAX: BCL2 associated X, apoptosis regulator; BECN1: beclin 1; BNIP3: BCL2 interacting protein 3; BNIP3L: BCL2 interacting protein 3 like; CASP3: caspase 3; CCK8: cell counting kit-8; CHT: chetomin; CMP: compression; CoCl: cobalt chloride; COL2A1: collagen type II alpha 1 chain; Ctrl: control; DAPI: 4,6-diamidino-2-phenylindole; DEP: differentially expressed protein; DiR: 1,1-dioctadecyl-3,3,3,3-tetramethyl indotricarbocyanine; ECM: extracellular matrix; FCM: flow cytometry; GD2: disialoganglioside GD 2; GFP: green fluorescent protein; GO: gene ontology; GSEA: gene set enrichment analysis; H&E: hematoxylin-eosin; HIF1A: hypoxia inducible factor 1 subunit alpha; HK2: hexokinase 2; HMOX1: heme oxygenase 1; HX: hypoxia mimicry; IDD: intervertebral disc degeneration; IF: immunofluorescence; IHC: immunohistochemistry; IVD: intervertebral disc; KEGG: kyoto encyclopedia of genes and genomes; LBP: low back pain; Lv: lentivirus; MAP1LC3B/LC3B: microtubule associated protein 1 light chain 3 beta; MMP: mitochondrial membrane potential; NC: negative control; NIR: near-infrared; NP: nucleus pulposus; NPC: nucleus pulposus cell; NPSC: nucleus pulposus derived stem cell; NX: normoxia; PPI: protein-protein interactions; RFP: red fluorescent protein; SLC2A1/GLUT1: solute carrier family 2 member 1; SQSTM1/p62: sequestosome 1; TEK/TIE2: TEK receptor tyrosine kinase; TEM: transmission electron microscopy; TUBB: tubulin beta class I.
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http://dx.doi.org/10.1080/15548627.2021.1872227DOI Listing
January 2021

Anti-Cancer Nanomedicines: A Revolution of Tumor Immunotherapy.

Front Immunol 2020 21;11:601497. Epub 2020 Dec 21.

Department of General Surgery, Zhuhai People's Hospital, Zhuhai Hospital Affiliated with Jinan University, Jinan University, Zhuhai, China.

Immunotherapies have been accelerating the development of anti-cancer clinical treatment, but its low objective responses and severe off-target immune-related adverse events (irAEs) limit the range of application. Strategies to remove these obstacles primarily focus on the combination of different therapies and the exploitation of new immunotherapeutic agents. Nanomedicine potentiates the effects of activating immune cells selectively and reversing tumor induced immune deficiency microenvironment through multiple mechanisms. In the last decade, a variety of nano-enabled tumor immunotherapies was under clinical investigation. As time goes by, the advantages of nanomedicine are increasingly prominent. With the continuous development of nanotechnology, nanomedicine will offer more distinctive perspectives in imaging diagnosis and treatment of tumors. In this Review, we wish to provide an overview of tumor immunotherapy and the mechanisms of nanomaterials that aim to enhance the efficacy of tumor immunotherapy under development or in clinic treatment.
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http://dx.doi.org/10.3389/fimmu.2020.601497DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7779686PMC
June 2021

COVID-19 emergencies around the globe: China's experience in controlling COVID-19 and lessons learned.

Int J Qual Health Care 2021 Feb;33(1)

Vice president, Vice secretary of Communist Party Committee, Zhuhai People's Hospital (Zhuhai Hospital affiliated with Jinan University), No. 79 Kangning Road, Xiangzhou District, Zhuhai, 519000, GP, China.

Motivation: Nations around the world have been significantly impacted during the COVID-19 pandemic. China's strategies for controlling COVID-19 offer valuable lessons for the global community. By learning from China's experience and lessons, other countries could also find appropriate methods to control the pandemic.

Problem Statement: What measures has China taken to control the pandemic? What lessons has China learned through this pandemic?

Approach/methods: The literature on China's lessons and experience in controlling the COVID-19 pandemic was searched and reviewed. Related newspapers and magazines were also searched.

Results: China's experience can be summed up as establishing temporary hospitals, strict isolation, experts with a knowledge of COVID-19, and measures that increase social distancing.

Conclusions: By learning from the experience of China, other countries in the world could eventually find the methods to control the COVID-19 pandemic. An emergency response system should be established in each country. Doctors and nurses are not alone in fighting COVID-19, and the entire world is helping them. With cooperation, current difficulties could be overcome.
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http://dx.doi.org/10.1093/intqhc/mzaa143DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7799047PMC
February 2021

Current therapeutic options for coronavirus disease 2019 (COVID-19)-lessons learned from severe acute respiratory syndrome (SARS) and Middle East Respiratory Syndrome (MERS) therapy: a systematic review protocol.

Ann Transl Med 2020 Nov;8(22):1527

Department of Pharmacy, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Background: Coronavirus disease 2019 (COVID-19), also known as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, first manifested in December 2019, and spread rapidly worldwide. Facing this lethal disease, there is an urgent need to develop potent therapies against SARS-CoV-2 infection. SARS-CoV-2 phylogenetically and symptomatically resembles SARS-CoV and Middle East Respiratory Syndrome Coronavirus (MERS-CoV). Numerous agents have been utilised during the severe acute respiratory syndrome (SARS) and Middle East Respiratory Syndrome (MERS) epidemics, which may show some benefit against SARS-CoV-2.

Methods: MEDLINE, EMBASE, Cochrane Library, CBM Disc, China National Knowledge Infrastructure, Wanfang Data, and the China Science and Technology Journal Database will be searched. Manual searches will be conducted by searching pre-printing websites, clinical trial registers, and screening the reference lists of inclusive studies. The screening of all citations and the selection of inclusive articles will be conducted by two reviewers. Randomised controlled trials (RCTs) and controlled cohort studies reporting antiviral therapies, including ribavirin, remdesivir, lopinavir/ritonavir, arbidol, chloroquine, hydroxychloroquine, and interferon, for SARS, MERS, and COVID-19 will be included. The primary outcomes will be mortality, incidence of acute respiratory distress syndrome, and utilisation of mechanical ventilation and intensive care unit admission. The secondary outcomes will be improvement in symptoms and chest radiography results, virus clearance, changes in blood test results, and serum tests. The quality of the retrieved RCTs and observational studies will be appraised according to the Cochrane risk of bias tool and the Newcastle-Ottawa Scale, respectively. If feasible, we will perform a fixed- or random-effects meta-analysis.

Discussion: This systematic review and meta-analysis will summarise all the available evidence for the efficacy and safety of current therapeutic options in SARS-CoV, MERS-CoV, or SARS-CoV-2-infected patients. The findings of this study may inform subsequent antiviral interventions for patients with COVID-19.

Study Registration: The protocol of this study has been submitted to the PROSPERO platform (https://www.crd.york.ac.uk/PROSPERO/), and the registration number is CRD42020168639.
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http://dx.doi.org/10.21037/atm-20-2340DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7729372PMC
November 2020

Role of PTHrP nuclear localization and carboxyl terminus sequences in postnatal spinal cord development.

Dev Neurobiol 2021 Jan 15;81(1):47-62. Epub 2020 Dec 15.

Department of Human Anatomy, Nanjing Medical University, Nanjing, P.R. China.

Parathyroid hormone-related peptide (PTHrP) acts under physiological conditions to regulate normal development of several tissues and organs. The role of PTHrP in spinal cord development has not been characterized. Pthrp knock in (Pthrp KI) mice were genetically modified to produce PTHrP in which there is a deficiency of the nuclear localization sequence (NLS) and C-terminus. Using this genetically modified mouse model, we have characterized its effect on spinal cord development early postnatally. The spinal cords from Pthrp KI mice displayed a significant reduction in its length, weight, and cross-sectional area compared to wild-type controls. Histologically, there was a decreased development of neurons and glial cells that caused decreased cell proliferation and increased apoptosis. The neural stem cells (NSCs) cultures also revealed decreased cell proliferation and differentiation and increased apoptosis. The proposed mechanism of delayed spinal cord development in Pthrp KI mice may be due to alteration in associated pathways in regulation of cell-division cycles and apoptosis. There was significant downregulation of Bmi-1 and upregulation of cyclin-dependent kinase inhibitors p27, p21, and p16 in Pthrp KI animals. We conclude that NLS and C-terminus peptide segments of PTHrP play an important role in inhibiting cell apoptosis and stimulation of cellular proliferation necessary for normal spinal cord development.
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http://dx.doi.org/10.1002/dneu.22798DOI Listing
January 2021

Effects of nutrition supplementation and physical exercise on muscle mass, muscle strength and fat mass among sarcopenic elderly: a randomized controlled trial.

Appl Physiol Nutr Metab 2021 May 20;46(5):494-500. Epub 2020 Nov 20.

Department of Clinical Nutrition and Department of Health Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences (CAMS), Peking Union Medical College (PUMC), Beijing 100730, China.

This study aimed to determine the impact of nutrition supplementation (whey protein, fish oil, vitamin D) and physical exercise (resistance and aerobic exercise) on muscle mass, muscle strength and fat mass among sarcopenic elderly. Participants ( = 241) with sarcopenia (aged ≥ 60 y) enrolled from 2 centers were randomized into groups undergoing nutrition supplementation (Nutr), physical exercise (Ex), nutrition combined with exercise (Nutr+Ex), or routine consultation for 12 weeks. Muscle-related indicators, grip strength as well as fat-related indicators were compared pre- and post-intervention. The per-protocol analysis showed that all indicators were significantly different between groups ( < 0.001). Further pairwise comparisons showed that compared with controls, appendicular muscle mass and grip strength were significantly higher in Nutr ( < 0.001, 95% CI: 0.578, 1.475; < 0.001, 95% CI: 3.614∼9.118), Ex ( = 0.010, 95% CI: 0.157, 1.153; < 0.001, 95% CI: 2.904, 8.732), and Nutr+Ex ( < 0.001, 95% CI: 0.564, 1.471; < 0.001, 95% CI: 3.441, 8.907) groups, while fat mass was significantly lower in the Nutr ( < 0.001, 95% CI: -4.676, -2.358) and Nutr+Ex ( < 0.001, 95% CI: -4.717,-1.790) groups. When compared with Ex, fat mass decreased significantly in the Nutr ( = 0.001, 95% CI: -4.856,-1.359) and Nutr+Ex ( = 0.005, 95% CI: -4.810, -0.878) groups. The findings indicate that nutrition supplementation and physical exercise could improve muscle mass, strength and fat mass among sarcopenic elderly. Nutrition supplementation might be better at decreasing fat mass in this population. ClinicalTrials.gov registration no.: NCT02873676. Nutrition supplementation improved muscle mass, strength and fat mass among sarcopenic elderly. Aerobic and resistance exercise improved muscle mass and strength in sarcopenic elderly. Combined nutrition supplementation and physical exercise improved muscle mass, strength and fat mass among sarcopenic elderly.
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http://dx.doi.org/10.1139/apnm-2020-0643DOI Listing
May 2021

Development of an informational support questionnaire of transitional care for aged patients with chronic disease.

BMJ Open 2020 11 17;10(11):e036573. Epub 2020 Nov 17.

Department of Information Management, Affiliated Hospital of Nantong University, Nantong, China.

Objectives: We developed an informational support questionnaire of transitional care (ISQTC) for aged patients with chronic disease and investigated its reliability and validity.

Setting: This study was conducted in three large general hospitals in Nantong, Jiangsu Province, China.

Participants: A total of 130 aged patients with chronic diseases, admitted into outpatient and inpatient departments from three hospitals in China, participated in the study. The inclusion criteria were: (1) patients must provide consent to participate; (2) being 60 years and above; (3) being diagnosed with at least one chronic disease and hospitalised more than two times within the last 1 year; (4) being able to listen, speak, read and write. The exclusion criteria were: (1) refusing to participate; (2) language expression and communication barriers (and having no caregiver to assist in participation); (3) being in intensive care or long-term hospitalisation.

Primary And Secondary Outcome Measures: The developed questionnaire was validated and tested for reliability. The content validity of the questionnaire was determined through experts' interviews and Delphi expert consultation, and the structure validity of the questionnaire was determined by performing exploratory factor analysis. The coefficient of reliability of the questionnaire was measured using Cronbach's alpha.

Results: Through Delphi expert consultation and exploratory factor analysis, the questionnaire was reduced from four dimensions and 12 items to three dimensions and 11 items. A total of 130 patients responded to the questionnaire. The alpha coefficient was 0.747.

Conclusion: The ISQTC is a reliable and valid instrument for evaluating aged patients with chronic disease in transitional care.

Trial Registration Details: ChiCTR1900020923. The trial was registered on 22 January 2019.
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http://dx.doi.org/10.1136/bmjopen-2019-036573DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7674111PMC
November 2020

Conventional thyroidectomy vs thyroid thermal ablation on postoperative quality of life and satisfaction for patients with benign thyroid nodules.

Eur J Endocrinol 2021 Jan;184(1):131-141

Zhuhai People's Hospital (Zhuhai Hospital Affiliated with Jinan University), Zhuhai, Guangdong Province, China.

Objective: The complications and treatment effects of conventional thyroidectomy and thyroid thermal ablation should be compared in order to identify the best intervention for patients with benign thyroid nodules.

Methods: Patients (18-50 years old) who had benign thyroid nodules and were eligible for both thyroidectomy and thyroid thermal ablation were randomly allocated (1:1) to either conventional thyroidectomy group or thyroid thermal ablation group. Patients' satisfaction and condition-specific quality of life were measured with the Thyroid-Specific Quality-of-Life Questionnaire Scale (QoL) at the 15th post-randomization month and were set as the co-primary outcome.

Results: A total of 450 patients were enrolled and randomized (225 patients in each group). At the 15th month after randomization, more patients in the thyroid thermal ablation group were satisfied with the treatment effects compared to those in the conventional thyroidectomy group. More patients in the thyroid thermal ablation group have a QoL score of 410 (QOL scores ranges from 0 to 410) than patients in conventional thyroidectomy. Eight (4%) of the 209 patients in conventional thyroidectomy group and 6 (3%) of the 208 patients in thyroid thermal ablation group had at least one severe postoperative complication. The time to achieve volume reduction was longer in the thermal ablation group.

Conclusion: Thyroid thermal ablation is superior to conventional thyroidectomy in terms of patients satisfaction, post-operative quality of life, and shorter hospital stay but takes longer to achieve BTNs volume reduction. The complication rates between the two groups were similar.
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http://dx.doi.org/10.1530/EJE-20-0562DOI Listing
January 2021

SOCS Proteins Participate in the Regulation of Innate Immune Response Caused by Viruses.

Front Immunol 2020 25;11:558341. Epub 2020 Sep 25.

Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.

The host immune system has multiple innate immune receptors that can identify, distinguish and react to viral infections. In innate immune response, the host recognizes pathogen-associated molecular patterns (PAMP) in nucleic acids or viral proteins through pathogen recognition receptors (PRRs), especially toll-like receptors (TLRs) and induces immune cells or infected cells to produce type I Interferons (IFN-I) and pro-inflammatory cytokines, thus when the virus invades the host, innate immunity is the earliest immune mechanism. Besides, cytokine-mediated cell communication is necessary for the proper regulation of immune responses. Therefore, the appropriate activation of innate immunity is necessary for the normal life activities of cells. The suppressor of the cytokine signaling proteins (SOCS) family is one of the main regulators of the innate immune response induced by microbial pathogens. They mainly participate in the negative feedback regulation of cytokine signal transduction through Janus kinase signal transducer and transcriptional activator (JAK/STAT) and other signal pathways. Taken together, this paper reviews the SOCS proteins structures and the function of each domain, as well as the latest knowledge of the role of SOCS proteins in innate immune caused by viral infections and the mechanisms by which SOCS proteins assist viruses to escape host innate immunity. Finally, we discuss potential values of these proteins in future targeted therapies.
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http://dx.doi.org/10.3389/fimmu.2020.558341DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7544739PMC
April 2021

Durable clinical benefit from pyrotinib combined with carboplatin in HER2-positive relapsed breast cancer previously treated with taxanes, anthracyclines, and trastuzumab.

Ann Palliat Med 2020 Sep;9(5):3684-3689

Zhuhai People's Hospital (Zhuhai Hospital Affiliated with Jinan University), Zhuhai, China.

Patients with human epidermal growth factor receptor 2 (HER2)-positive breast cancer inevitably progressed after a short response to initial trastuzumab treatment, suggesting a possibility of acquired-resistance to trastuzumab. Pyrotinib, an irreversible pan-ErbB receptor tyrosine kinase inhibitor (TKI), has been reported as an effective and safe drug for the treatment of HER2-positive relapsed or metastatic breast cancer. Pyrotinib combined with capecitabine is widely used to treat HER2-positive metastatic breast cancer in patients who have been previously treated with anthracyclines, taxanes, and trastuzumab. However, the efficacy of pyrotinib combined with other chemotherapy drugs is still unclear. Here we report pyrotinib combined with carboplatin in treating a patient with HER2-positive relapsed breast cancer who had acquired resistance to trastuzumab. The patient received three cycles of treatments of pyrotinib (400 mg, orally once per day, days 1-21) combined with carboplatin (600 mg, iv drip, day 1, cycled every 21 days). The patient showed an excellent response to the therapy, including faded rashes on the skin of her breast, no obvious signs of recurrence from the breast magnetic resonance imaging (MRI), decreased skin thickness and cord shadow of the right breast, unchanged degree of right pleural effusion, and no enlarged LN. The patient had a stable disease time of more than four months. Our case provides evidence for the feasibility and efficacy of pyrotinib with carboplatin in treating patients with HER2-positive relapsed or metastatic breast cancer who may develop resistance to trastuzumab.
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http://dx.doi.org/10.21037/apm-20-1363DOI Listing
September 2020

Association between dietary antioxidants and risk for diabetic retinopathy in a Chinese population.

Eye (Lond) 2020 Oct 2. Epub 2020 Oct 2.

Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing, China.

Background: Diabetic retinopathy (DR) is related to oxidative stress and insufficient intake of dietary antioxidants may be associated with the onset and progression of DR. This study aimed to detect the association between main dietary antioxidants intake and the risk for DR.

Methods: This is a cross-sectional study of a Chinese urban population. Four hundred and fifty-five subjects with type 2 diabetes were recruited and divided into diabetic patients without retinopathy (DWR) group and DR group based on their retinal status. CSMO (clinically significant macular oedema) was diagnosed by stereoscopic photography. Demographic and lifestyle characteristics were ascertained by questionnaire. General physical and ophthalmic examinations were completed for all subjects. Dietary antioxidants were assessed by 3-day food records. Subjects who have taken any type of vitamin supplements were excluded from the study. The association of dietary antioxidants with the risk for DR was analysed by logistic regression with adjustment of other factors. The dietary antioxidants levels of the CSMO subjects and non-CSMO subjects were compared using the Wilcoxon rank sum test.

Results: One hundred and nineteen subjects in DR group and 336 subjects in DWR group participated in the study. Only ten DR subjects had CSMO. The results showed that higher vitamin E (OR (95% CI):0.97 (0.95, 1.00), P = 0.036) and selenium (OR (95% CI):0.98 (0.96, 1.00), P = 0.017) intake appear to be the protective factors of DR. The dietary antioxidants levels of CSMO and non-CSMO subjects had no statistical differences (P > 0.05).

Conclusions: Dietary antioxidants intake, particularly vitamin E and selenium, were observed to have protective effects on DR.
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http://dx.doi.org/10.1038/s41433-020-01208-zDOI Listing
October 2020

Evolutionary dynamics and transmission patterns of Newcastle disease virus in China through Bayesian phylogeographical analysis.

PLoS One 2020 29;15(9):e0239809. Epub 2020 Sep 29.

Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, People's Republic of China.

The Chinese poultry industry has experienced outbreaks of Newcastle disease (ND) dating back to the 1920s. However, the epidemic has exhibited a downtrend in recent years. In this study, both observational and genetic data [fusion (F) and haemagglutinin-neuraminidase genes (HN)] were analyzed, and phylogeographic analysis based on prevalent genotypes of Newcastle disease virus (NDV) was conducted for better understanding of the evolution and spatiotemporal dynamics of ND in China. In line with the observed trend of epidemic outbreaks, the effective population size of F and HN genes of circulating NDV is no longer growing since 2000, which is supported by 95% highest posterior diversity (HPD) intervals. Phylogeographic analysis indicated that the two eastern coastal provinces, Shandong and Jiangsu were the most relevant hubs for NDV migration, and the geographical regions with active NDV diffusion seemed to be constrained to southern and eastern China. The live poultry trade may play an important role in viral spread. Interestingly, no migration links from wild birds to poultry received Bayes factor support (BF > 3), while the migration links from poultry to wild birds accounted for 64% in all effective migrations. This may indicate that the sporadic cases of ND in wild bird likely spillover events from poultry. These findings contribute to predictive models of NDV transmission, and potentially help in the prevention of future outbreaks.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0239809PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7523974PMC
November 2020