Miguel Alcalde

Miguel Alcalde

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Miguel Alcalde

Miguel Alcalde

Publications by authors named "Miguel Alcalde"

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The Generation of Thermostable Fungal Laccase Chimeras by SCHEMA-RASPP Structure-Guided Recombination in Vivo.

ACS Synth Biol 2019 Apr 1;8(4):833-843. Epub 2019 Apr 1.

Department of Biocatalysis , Institute of Catalysis, CSIC , Cantoblanco , 28049 Madrid , Spain.

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http://pubs.acs.org/doi/10.1021/acssynbio.8b00509
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http://dx.doi.org/10.1021/acssynbio.8b00509DOI Listing
April 2019

Structure-Guided Immobilization of an Evolved Unspecific Peroxygenase.

Int J Mol Sci 2019 Apr 2;20(7). Epub 2019 Apr 2.

Department of Biocatalysis, Institute of Catalysis, CSIC, Cantoblanco, 28049 Madrid, Spain.

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https://www.mdpi.com/1422-0067/20/7/1627
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http://dx.doi.org/10.3390/ijms20071627DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6480235PMC
April 2019

Multienzymatic in situ hydrogen peroxide generation cascade for peroxygenase-catalysed oxyfunctionalisation reactions.

Z Naturforsch C 2019 Feb;74(3-4):101-104

Department of Biotechnology, Delft University of Technology, van der Maasweg 9, 2629HZ Delft, The Netherlands.

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http://dx.doi.org/10.1515/znc-2018-0137DOI Listing
February 2019

Expanding the Spectrum of Light-Driven Peroxygenase Reactions.

ACS Catal 2019 Feb 18;9(2):890-894. Epub 2018 Dec 18.

Department of Biotechnology, Delft University of Technology, van der Maasweg 9, 2629 HZ Delft, The Netherlands.

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http://dx.doi.org/10.1021/acscatal.8b03752DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6369655PMC
February 2019

Structural Insights into the Substrate Promiscuity of a Laboratory-Evolved Peroxygenase.

ACS Chem Biol 2018 12 16;13(12):3259-3268. Epub 2018 Nov 16.

Department of Biocatalysis, Institute of Catalysis , CSIC , 28049 Madrid , Spain.

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http://pubs.acs.org/doi/10.1021/acschembio.8b00500
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http://dx.doi.org/10.1021/acschembio.8b00500DOI Listing
December 2018

Shuffling the Neutral Drift of Unspecific Peroxygenase in Saccharomyces cerevisiae.

Appl Environ Microbiol 2018 08 17;84(15). Epub 2018 Jul 17.

Department of Biocatalysis, Institute of Catalysis, CSIC, Madrid, Spain

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http://dx.doi.org/10.1128/AEM.00808-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6052263PMC
August 2018

Functional expression of aryl-alcohol oxidase in Saccharomyces cerevisiae and Pichia pastoris by directed evolution.

Biotechnol Bioeng 2018 07 30;115(7):1666-1674. Epub 2018 Mar 30.

Department of Biocatalysis, Institute of Catalysis, CSIC, Cantoblanco, Madrid, Spain.

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http://dx.doi.org/10.1002/bit.26585DOI Listing
July 2018

Beyond the outer limits of nature by directed evolution.

Biotechnol Adv 2016 Sep-Oct;34(5):754-767. Epub 2016 Apr 5.

Department of Biocatalysis, Institute of Catalysis, CSIC, Cantoblanco, 28049 Madrid, Spain. Electronic address:

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http://dx.doi.org/10.1016/j.biotechadv.2016.03.008DOI Listing
December 2017

Laccase: a multi-purpose biocatalyst at the forefront of biotechnology.

Microb Biotechnol 2017 11 3;10(6):1457-1467. Epub 2016 Oct 3.

Department of Biocatalysis, Institute of Catalysis, CSIC, Cantoblanco, 28049, Madrid, Spain.

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http://dx.doi.org/10.1111/1751-7915.12422DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5658592PMC
November 2017

Evolved α-factor prepro-leaders for directed laccase evolution in Saccharomyces cerevisiae.

Microb Biotechnol 2017 11 14;10(6):1830-1836. Epub 2017 Aug 14.

Department of Biocatalysis, Institute of Catalysis, CSIC, Cantoblanco, 28049, Madrid, Spain.

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http://dx.doi.org/10.1111/1751-7915.12838DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5658585PMC
November 2017

Selective Activation of C-H Bonds in a Cascade Process Combining Photochemistry and Biocatalysis.

Angew Chem Int Ed Engl 2017 11 3;56(48):15451-15455. Epub 2017 Nov 3.

Department of Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629HZ, Delft, The Netherlands.

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http://dx.doi.org/10.1002/anie.201708668DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5725739PMC
November 2017

Modification of the peroxygenative:peroxidative activity ratio in the unspecific peroxygenase from Agrocybe aegerita by structure-guided evolution.

Protein Eng Des Sel 2017 03;30(3):189-196

Department of Biocatalysis, Institute of Catalysis, CSIC, Marie Curie 2, 28049 Madrid, Spain.

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http://dx.doi.org/10.1093/protein/gzw073DOI Listing
March 2017

When directed evolution met ancestral enzyme resurrection.

Authors:
Miguel Alcalde

Microb Biotechnol 2017 01 11;10(1):22-24. Epub 2016 Nov 11.

Department of Biocatalysis, Institute of Catalysis, CSIC, Cantoblanco, 28049, Madrid, Spain.

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http://dx.doi.org/10.1111/1751-7915.12452DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5270717PMC
January 2017

Unveiling the basis of alkaline stability of an evolved versatile peroxidase.

Biochem J 2016 07 26;473(13):1917-28. Epub 2016 Apr 26.

Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, E-28040 Madrid, Spain

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http://dx.doi.org/10.1042/BCJ20160248DOI Listing
July 2016

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening.

J Vis Exp 2016 Apr 1(110):e53761. Epub 2016 Apr 1.

Department of Biocatalysis, Institute of Catalysis, CSIC;

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http://dx.doi.org/10.3791/53761DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4841332PMC
April 2016

Mutagenic Organized Recombination Process by Homologous IN vivo Grouping (MORPHING) for directed enzyme evolution.

PLoS One 2014 10;9(3):e90919. Epub 2014 Mar 10.

Departmento de Biocatálisis, Instituto de Catálisis y Petroleoquímica, Consejo Superior de Investigaciones Científicas (CSIC), Madrid, Spain.

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0090919PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3948698PMC
March 2016

Synthesis of 1-Naphthol by a Natural Peroxygenase Engineered by Directed Evolution.

Chembiochem 2016 Feb 21;17(4):341-9. Epub 2016 Jan 21.

Department of Biocatalysis, Institute of Catalysis, CSIC, C/Marie Curie n° 2L10, 28049, Madrid, Spain.

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http://dx.doi.org/10.1002/cbic.201500493DOI Listing
February 2016

Peroxygenase-Catalyzed Oxyfunctionalization Reactions Promoted by the Complete Oxidation of Methanol.

Angew Chem Int Ed Engl 2016 Jan 26;55(2):798-801. Epub 2015 Nov 26.

Department of Biotechnology, Delft University of Technology, Julianalaan 136, 2628BL Delft (The Netherlands).

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http://dx.doi.org/10.1002/anie.201507881DOI Listing
January 2016

Laccase engineering: from rational design to directed evolution.

Biotechnol Adv 2015 Jan-Feb;33(1):25-40. Epub 2014 Dec 27.

Department of Biocatalysis, Institute of Catalysis, CSIC, Cantoblanco, 28049 Madrid, Spain. Electronic address:

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http://dx.doi.org/10.1016/j.biotechadv.2014.12.007DOI Listing
December 2015

Focused Directed Evolution of Aryl-Alcohol Oxidase in Saccharomyces cerevisiae by Using Chimeric Signal Peptides.

Appl Environ Microbiol 2015 Sep 10;81(18):6451-62. Epub 2015 Jul 10.

Department of Biocatalysis, Institute of Catalysis, CSIC, Madrid, Spain

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http://dx.doi.org/10.1128/AEM.01966-15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4542223PMC
September 2015

Tandem-yeast expression system for engineering and producing unspecific peroxygenase.

Enzyme Microb Technol 2015 Jun 23;73-74:29-33. Epub 2015 Mar 23.

Department of Biocatalysis, Institute of Catalysis, CSIC, 28049 Madrid, Spain. Electronic address:

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https://linkinghub.elsevier.com/retrieve/pii/S01410229150004
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http://dx.doi.org/10.1016/j.enzmictec.2015.03.004DOI Listing
June 2015

Assembly of evolved ligninolytic genes in Saccharomyces cerevisiae.

Bioengineered 2014 Jul-Aug;5(4):254-63. Epub 2014 May 15.

Department of Biocatalysis; Institute of Catalysis, CSIC; Madrid, Spain.

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http://dx.doi.org/10.4161/bioe.29167DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4143396PMC
May 2015

Engineering the ligninolytic enzyme consortium.

Authors:
Miguel Alcalde

Trends Biotechnol 2015 Mar 15;33(3):155-62. Epub 2015 Jan 15.

Department of Biocatalysis, Institute of Catalysis, Consejo Superior de Investigaciones Científicas (CSIC), 28049 Madrid, Spain. Electronic address:

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http://dx.doi.org/10.1016/j.tibtech.2014.12.007DOI Listing
March 2015

Directed evolution of unspecific peroxygenase from Agrocybe aegerita.

Appl Environ Microbiol 2014 Jun 28;80(11):3496-507. Epub 2014 Mar 28.

Department of Biocatalysis, Institute of Catalysis, CSIC, Madrid, Spain.

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http://dx.doi.org/10.1128/AEM.00490-14DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4018863PMC
June 2014

Bioelectrochemical oxidation of water.

J Am Chem Soc 2014 Apr 11;136(16):5892-5. Epub 2014 Apr 11.

Instituto de Catalisis y Petroleoquimica, CSIC , C/Marie Curie, 2, L10, 28049 Madrid, Spain.

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http://dx.doi.org/10.1021/ja502044jDOI Listing
April 2014

New colorimetric screening assays for the directed evolution of fungal laccases to improve the conversion of plant biomass.

BMC Biotechnol 2013 Oct 26;13:90. Epub 2013 Oct 26.

Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, 28040 Madrid, Spain.

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http://dx.doi.org/10.1186/1472-6750-13-90DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4015961PMC
October 2013

Widening the pH activity profile of a fungal laccase by directed evolution.

Chembiochem 2013 May 16;14(8):934-7. Epub 2013 Apr 16.

Department of Biocatalysis, Institute of Catalysis, CSIC, Marie Curie St. 2, 28049 Madrid, Spain.

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http://dx.doi.org/10.1002/cbic.201300102DOI Listing
May 2013

Semi-rational engineering of cellobiose dehydrogenase for improved hydrogen peroxide production.

Microb Cell Fact 2013 Apr 23;12:38. Epub 2013 Apr 23.

Vienna Institute of Biotechnology, Department of Food Sciences and Technology, BOKU-University of Natural Resources and Life Sciences, Vienna, Austria.

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http://dx.doi.org/10.1186/1475-2859-12-38DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3654988PMC
April 2013

Functional expression of a blood tolerant laccase in Pichia pastoris.

BMC Biotechnol 2013 Apr 30;13:38. Epub 2013 Apr 30.

Department of Biocatalysis, Institute of Catalysis, CSIC, Madrid 28049, Spain.

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http://dx.doi.org/10.1186/1472-6750-13-38DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3655043PMC
April 2013

Blood tolerant laccase by directed evolution.

Chem Biol 2013 Feb;20(2):223-31

Department of Biocatalysis, Institute of Catalysis, CSIC, 28049 Madrid, Spain.

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https://linkinghub.elsevier.com/retrieve/pii/S10745521130000
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http://dx.doi.org/10.1016/j.chembiol.2013.01.001DOI Listing
February 2013

Development of chimeric laccases by directed evolution.

Biotechnol Bioeng 2012 Dec 12;109(12):2978-86. Epub 2012 Jul 12.

Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, 28040 Madrid, Spain.

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http://dx.doi.org/10.1002/bit.24588DOI Listing
December 2012

Saccharomyces cerevisiae in directed evolution: An efficient tool to improve enzymes.

Bioeng Bugs 2012 May-Jun;3(3):172-7. Epub 2012 May 1.

Department of Biocatalysis, Institute of Catalysis, CSIC, Madrid, Spain.

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http://dx.doi.org/10.4161/bbug.19544DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3370936PMC
October 2012

Risk of recurrence of non-metastatic breast cancer in women under 40 years: a population-registry cancer study in a European country.

Breast J 2012 Mar-Apr;18(2):118-23. Epub 2011 Dec 30.

General and Digestive Surgery, Castellon General Hospital, Castellon, Spain.

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http://dx.doi.org/10.1111/j.1524-4741.2011.01208.xDOI Listing
August 2012

Directed evolution of a temperature-, peroxide- and alkaline pH-tolerant versatile peroxidase.

Biochem J 2012 Jan;441(1):487-98

Department of Biocatalysis, Institute of Catalysis, CSIC, Cantoblanco, 28049 Madrid, Spain.

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http://dx.doi.org/10.1042/BJ20111199DOI Listing
January 2012

Screening β-fructofuranosidases mutant libraries to enhance the transglycosylation rates of β-(2→6) fructooligosaccharides.

Comb Chem High Throughput Screen 2011 Sep;14(8):730-8

Centro de Biología Molecular Severo Ochoa, Departamento de Biología Molecular (UAM-CSIC), Universidad Autónoma Madrid, Madrid, Spain.

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September 2011

Directed evolution of fungal laccases.

Curr Genomics 2011 Apr;12(2):113-22

Department of Biocatalysis, Institute of Catalysis, CSIC, Cantoblanco, 28049 Madrid, Spain.

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http://dx.doi.org/10.2174/138920211795564322DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3129045PMC
April 2011

Mutagenesis protocols in Saccharomyces cerevisiae by in vivo overlap extension.

Authors:
Miguel Alcalde

Methods Mol Biol 2010 ;634:3-14

Instituto de Catálisis y Petroleoquímica (CSIC), Madrid, Spain.

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http://dx.doi.org/10.1007/978-1-60761-652-8_1DOI Listing
December 2010

Laboratory evolution of high-redox potential laccases.

Chem Biol 2010 Sep;17(9):1030-41

Department of Biocatalysis, Institute of Catalysis, CSIC, Cantoblanco, Madrid, Spain.

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http://dx.doi.org/10.1016/j.chembiol.2010.07.010DOI Listing
September 2010

Evolving thermostability in mutant libraries of ligninolytic oxidoreductases expressed in yeast.

Microb Cell Fact 2010 Mar 18;9:17. Epub 2010 Mar 18.

Department of Biocatalysis, Institute of Catalysis, Madrid, Spain.

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http://dx.doi.org/10.1186/1475-2859-9-17DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2847542PMC
March 2010

Laccases and their applications: a patent review.

Recent Pat Biotechnol 2008 ;2(1):10-24

Departamento de Biocatálisis, Instituto de Catálisis y Petroleoquímica, CSIC, Cantoblanco, 28049 Madrid, Spain.

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March 2009

Engineering and Applications of fungal laccases for organic synthesis.

Microb Cell Fact 2008 Nov 20;7:32. Epub 2008 Nov 20.

Departamento de Biocatálisis, Instituto de Catálisis y Petroleoquímica, CSIC, Marie Curie 2, 28049 Madrid, Spain.

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http://dx.doi.org/10.1186/1475-2859-7-32DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2613868PMC
November 2008

Altering the laccase functionality by in vivo assembly of mutant libraries with different mutational spectra.

Proteins 2008 Apr;71(1):250-60

Instituto de Catálisis y Petroleoquímica, CSIC, Cantoblanco, Madrid 28049, Spain.

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http://dx.doi.org/10.1002/prot.21699DOI Listing
April 2008

In vitro evolution of a fungal laccase in high concentrations of organic cosolvents.

Chem Biol 2007 Sep;14(9):1052-64

Department of Biocatalysis, Institute of Catalysis, Consejo Superior de Investigaciones Cientificas, Cantoblanco, 28049 Madrid, Spain.

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http://dx.doi.org/10.1016/j.chembiol.2007.08.010DOI Listing
September 2007

Transformation of polycyclic aromatic hydrocarbons by laccase is strongly enhanced by phenolic compounds present in soil.

Environ Sci Technol 2007 Apr;41(8):2964-71

Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, 28040 Madrid, Spain.

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http://dx.doi.org/10.1021/es062328jDOI Listing
April 2007

Combinatorial saturation mutagenesis by in vivo overlap extension for the engineering of fungal laccases.

Comb Chem High Throughput Screen 2006 Dec;9(10):719-27

Departamento de Biocatálisis, Instituto de Catálisis y Petroleoquímica, CSIC, Cantoblanco 28049, Madrid, Spain.

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December 2006

Synthesis of methyl alpha-D-glucooligosaccharides by entrapped dextransucrase from Leuconostoc mesenteroides B-1299.

J Biotechnol 2006 Jul 2;124(2):439-45. Epub 2006 Mar 2.

Departamento de Biocatálisis, Instituto de Catálisis y Petroleoquímica, CSIC, Cantoblanco, Madrid, Spain.

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http://dx.doi.org/10.1016/j.jbiotec.2005.12.031DOI Listing
July 2006

Environmental biocatalysis: from remediation with enzymes to novel green processes.

Trends Biotechnol 2006 Jun 2;24(6):281-7. Epub 2006 May 2.

Departmento de Biocatálisis, Instituto de Catálisis y Petroleoquímica, CSIC, Cantoblanco, 28049 Madrid, Spain.

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http://dx.doi.org/10.1016/j.tibtech.2006.04.002DOI Listing
June 2006

Beet sugar syrup and molasses as low-cost feedstock for the enzymatic production of fructo-oligosaccharides.

J Agric Food Chem 2006 Apr;54(8):2964-8

Departamento de Biocatalisis, Instituto de Catalisis y Petroleoquímica, CSIC, Cantoblanco, 28049 Madrid, Spain.

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http://dx.doi.org/10.1021/jf053023bDOI Listing
April 2006

Screening mutant libraries of fungal laccases in the presence of organic solvents.

J Biomol Screen 2005 Sep 15;10(6):624-31. Epub 2005 Aug 15.

Departamento de Biocatálisis, Instituto de Catálisis y Petroleoquímica, CSIC, Madrid, Spain.

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http://dx.doi.org/10.1177/1087057105277058DOI Listing
September 2005

Immobilization of dextransucrase from Leuconostoc mesenteroides NRRL B-512F on Eupergit C supports.

Biotechnol Prog 2004 Sep-Oct;20(5):1414-20

Departamento de Biocatálisis and Departamento de Ingeniería de Procesos Catalíticos, Instituto de Catálisis, CSIC, Cantoblanco, 28049 Madrid, Spain.

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http://dx.doi.org/10.1021/bp0400083DOI Listing
May 2005

Use and improvement of microbial redox enzymes for environmental purposes.

Microb Cell Fact 2004 Aug 2;3(1):10. Epub 2004 Aug 2.

Departamento de Biocatálisis, Instituto de Catálisis, CSIC, Cantoblanco, 28049 Madrid, Spain.

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http://dx.doi.org/10.1186/1475-2859-3-10DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC509422PMC
August 2004

Effect of carbohydrate fatty acid esters on Streptococcus sobrinus and glucosyltransferase activity.

Carbohydr Res 2004 Apr;339(6):1029-34

School of Dentistry, University of Southern California, 925 W 34th Street, Los Angeles, CA 90089-0641, USA.

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http://dx.doi.org/10.1016/j.carres.2004.01.007DOI Listing
April 2004

Colorimetric high-throughput assay for alkene epoxidation catalyzed by cytochrome P450 BM-3 variant 139-3.

J Biomol Screen 2004 Mar;9(2):141-6

Departamento de Biocatalisis, Instituto de Catalisis y Petroleoquimica (CSIC), Campus Universidad Autonoma de Madrid, Cantoblanco, Madrid, Spain.

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http://dx.doi.org/10.1177/1087057103261913DOI Listing
March 2004

Preparing libraries in Saccharomyces cerevisiae.

Methods Mol Biol 2003 ;231:17-22

Department of Chemical Engineering, University of California at Los Angeles, USA.

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http://dx.doi.org/10.1385/1-59259-395-X:17DOI Listing
November 2003

Screening mutant libraries in Saccharomyces cerevisiae.

Methods Mol Biol 2003 ;230:99-107

Department of Chemical Engineering, University of California at Los Angeles, Los Angeles, CA, USA.

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http://dx.doi.org/10.1385/1-59259-396-8:99DOI Listing
October 2003

Colorimetric assays for screening laccases.

Methods Mol Biol 2003 ;230:193-201

Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA, USA.

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http://dx.doi.org/10.1385/1-59259-396-8:193DOI Listing
October 2003

Functional expression of a fungal laccase in Saccharomyces cerevisiae by directed evolution.

Appl Environ Microbiol 2003 Feb;69(2):987-95

Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125,USA.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC143632PMC
http://dx.doi.org/10.1128/aem.69.2.987-995.2003DOI Listing
February 2003

Colorimetric assays for biodegradation of polycyclic aromatic hydrocarbons by fungal laccases.

J Biomol Screen 2002 Dec;7(6):547-53

Division of Chemistry and Chemical Engineering 210-41, California Institute of Technology, Pasadena, CA, USA.

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http://dx.doi.org/10.1177/1087057102238629DOI Listing
December 2002

Covalent immobilization of cyclodextrin glucosyltransferase (CGTase) in activated silica and Sepharose.

Indian J Biochem Biophys 2002 Aug;39(4):229-34

Departamento de Biocatálisis, Instituto de Catálisis, CSIC, Campus UAM Cantoblanco, 28049 Madrid, Spain.

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August 2002

Modification of galactose oxidase to introduce glucose 6-oxidase activity.

Chembiochem 2002 Aug;3(8):781-3

Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA.

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http://dx.doi.org/10.1002/1439-7633(20020802)3:8<781::AID-CBIC781>3.0.CO;2-8DOI Listing
August 2002