Publications by authors named "Michał Pac"

4 Publications

  • Page 1 of 1

Selective Biological Effects of Selenium-Enriched Polysaccharide (Se-Le-30) Isolated from Mycelium on Human Immune Cells.

Biomolecules 2021 11 26;11(12). Epub 2021 Nov 26.

Department of Drug Technology and Pharmaceutical Biotechnology, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland.

A common edible mushroom is an important source of numerous biologically active substances, including polysaccharides, with immunomodulatory and antitumor properties. In the present work, the biological activity of the crude, homogenous (Se)-enriched fraction (named Se-Le-30), which has been isolated from mycelium by a modified Chihara method towards human peripheral blood mononuclear cells (PBMCs) and peripheral granulocytes, was investigated. The Se-Le-30 fraction, an analog of lentinan, significantly inhibited the proliferation of human PBMCs stimulated with anti-CD3 antibodies or allostimulated, and down-regulated the production of tumor necrosis factor (TNF)-α by CD3+ T cells. Moreover, it was found that Se-Le-30 significantly reduced the cytotoxic activity of human natural killer (NK) cells. The results suggested the selective immunosuppressive activity of this fraction, which is non-typical for mushroom derived polysaccharides.
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http://dx.doi.org/10.3390/biom11121777DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8698628PMC
November 2021

Glucocorticoid Receptor Gene Polymorphisms Are Associated with Membranous and IgA Nephropathies.

Cells 2021 11 16;10(11). Epub 2021 Nov 16.

Department of Immunology, Transplantology and Internal Diseases, Medical University of Warsaw, 02-006 Warsaw, Poland.

Glomerular diseases (GNs) are responsible for approximately 20% of chronic kidney diseases. Glucocorticoid receptor gene () single nucleotide polymorphisms (SNPs) are implicated in differences in predisposition to autoimmunity and steroid sensitivity. The aim of this study was to evaluate the frequency of the SNPs-rs6198, rs41423247 and rs17209237-in 72 IgA nephropathy (IgAN) and 38 membranous nephropathy (MN) patients compared to 175 healthy controls and to correlate the effectiveness of treatment in IgAN and MN groups defined as a reduction of proteinuria <1 g/24 h after 12 months of treatment. Real-time polymerase chain reactions and SNP array-based typing were used. We found significant rs41423247 association with MN ( = 0.026); a significant association of rs17209237 with eGFR reduction after follow-up period in all patients with GNs ( = 0.021) and with the degree of proteinuria after 1 year of therapy in all patients with a glomerulopathy ( = 0.013) and IgAN ( = 0.021); and in the same groups treated with steroids ( = 0.021; = 0.012). We also observed the association between rs41423247 and IgAN histopathologic findings ( = 0.012). In conclusion, our results indicate that polymorphisms may influence treatment susceptibility and clinical outcome in IgAN and MN.
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http://dx.doi.org/10.3390/cells10113186DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8625873PMC
November 2021

Kidney length normative values in children aged 0-19 years - a multicenter study.

Pediatr Nephrol 2021 Oct 16. Epub 2021 Oct 16.

Department of Nephrology, Kidney Transplantation and Hypertension, Children's Memorial Health Institute, al. Dzieci Polskich 20, 04-730, Warsaw, Poland.

Background: Currently used pediatric kidney length normative values are based on small single-center studies, do not include kidney function assessment, and focus mostly on newborns and infants. We aimed to develop ultrasound-based kidney length normative values derived from a large group of European Caucasian children with normal kidney function.

Methods: Out of 1,782 children aged 0-19 years, 1,758 individuals with no present or past kidney disease and normal estimated glomerular filtration rate had sonographic assessment of kidney length. The results were correlated with anthropometric parameters and estimated glomerular filtration rate. Kidney length was correlated with age, height, body surface area, and body mass index. Height-related kidney length curves and table were generated using the LMS method. Multivariate regression analysis with collinearity checks was used to evaluate kidney length predictors.

Results: There was no significant difference in kidney size in relation to height between boys and girls. We found significant (p < 0.001), but clinically unimportant (Cohen's D effect size = 0.04 and 0.06) differences between prone vs. supine position (mean paired difference = 0.64 mm, 95% CI = 0.49-0.77) and left vs. right kidneys (mean paired difference = 1.03 mm, 95% CI = 0.83-1.21), respectively. For kidney length prediction, the highest coefficient correlation was observed with height (adjusted R = 0.87, p < 0.0001).

Conclusions: We present height-related LMS-percentile curves and tables of kidney length which may serve as normative values for kidney length in children from birth to 19 years of age. The most significant predictor of kidney length was statural height.
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http://dx.doi.org/10.1007/s00467-021-05303-5DOI Listing
October 2021

Circulating Osteoprotegerin in Chronic Kidney Disease and All-Cause Mortality.

Int J Gen Med 2021 9;14:2413-2420. Epub 2021 Jun 9.

Department of Immunology, Transplantology and Internal Diseases, Medical University of Warsaw, Warsaw, Poland.

Background: Chronic kidney disease (CKD) is associated with cardiovascular disease (CKD), mineral and bone disorder (CKD-MBD) and high mortality. Bone-related factors such as osteopontin (OPN), osteocalcin (OC), osteoprotegerin (OPG) and fibroblast growth factor 23 (FGF23) were linked to cardiovascular complications of CKD and are expected to have predictive value in CKD patients.

Purpose: The aim of this study was to assess the relationship of OPN, OC, OPG and FGF23 to clinical characteristics and to evaluate their ability to predict mortality in patients with different CKD stages.

Methods: The following study groups were enrolled: subjects with end-stage renal disease (38 ESRD), CKD stages 3 and 4 (19 CKD3-4) and non-CKD controls (19), respectively. Blood was withdrawn once to perform the measurements and cardiac computed tomography was used to evaluate coronary calcium score (CS). Patients were followed for 5 years for the ascertainment of their all-cause mortality.

Results: Serum OPN, OC and OPG concentrations increased significantly along with the progression of renal disease. We found a significant positive correlation among these proteins. Additionally, OPN and OPG were significantly and positively correlated to CS. Serum OPG revealed the strongest correlation to the calcium turnover markers of GFR decline and was significantly associated with an increased risk of death in subjects with CKD3-4 or ESRD (HR 5.8, CI 95%).

Conclusion: Single measurement of osteoprotegerin is associated with 5-year all-cause mortality in patients with CKD3-4 or ESRD. We suggest assessing its concentration, preferably in combination with calcium score, to stratify mortality risks in CKD patients.
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http://dx.doi.org/10.2147/IJGM.S302251DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8200134PMC
June 2021
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