Publications by authors named "Michał Kulus"

6 Publications

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Assessing weaning stress - Relations between enamel hypoplasia, δO and δC values in human teeth obtained from early modern cemeteries in Wroclaw, Poland.

Ann Anat 2020 Nov 20;232:151546. Epub 2020 Jun 20.

Institute of Geological Sciences, Polish Academy of Sciences, Research Centre in Warszawa, Wroclaw, Poland.

Objectives: The aim of this paper is to assess linear enamel hypoplasia (LEH) on canines of adults from early modern Wroclaw cemeteries to compare δC and δO values in enamel formed before, during and after LEH formation, and to attempt to find a link between the selected indicators of the weaning process.

Material And Methods: 15 permanent canines with visible LEH were selected for analysis. Age at LEH formation was estimated using the Reid and Dean method. The perikymata count was used to estimate the duration of LEH formation. Incremental stable oxygen and carbon isotopes analysis was performed on tooth enamel to infer stress related to weaning and dietary patterns. Kruskal-Wallis ANOVA, Mann-Whitney U test and Spearman correlation were used in statistical inference.

Results: The average age of LEH formation was 3.14 years, with an average duration of 111 days. Stable carbon isotope values consistently increase in 73% of the individuals sampled. The values of δO do not show a dominant pattern. The average value of δC increases over the time of enamel formation. The δC values collected below LEH were negatively correlated with δO values and the duration of LEH termination.

Conclusions: Marking of carbon and oxygen isotopes indicates different stages of weaning. Advancements in diet change (determined by higher δC) corresponds with faster recovery after physiological stress episode. There is no evidence for different breastfeeding models between distinct parishes in early modern Wroclaw.
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http://dx.doi.org/10.1016/j.aanat.2020.151546DOI Listing
November 2020

A Case of Syphilis with High Bone Arsenic Concentration from Early Modern Cemetery (Wroclaw, Poland).

Open Life Sci 2019 Jan 17;14:427-439. Epub 2019 Nov 17.

Department of Human Morphology and Embryology, Division of Histology and Embryology, Wroclaw Medical University, ul. Chalubinskiego 6a, 50-368 Wroclaw, Poland.

Venereal syphilis is a sexually transmitted disease caused by Treponema pallidum - Gram-negative, slowly growing bacteria. The spread of the disease in the Old World was due to increased birth rate, urban population growth, migration and lack of knowledge concerning the epidemiology. In the past, the treatment was mainly symptomatic and included application of mercury compounds. The goal of the study was to present the case of advanced venereal syphilis found in early modern (16th-18thc) graveyard localized in Wroclaw, Poland. The object of the study is a cranium of a male whose age at death has been estimated to be over 55. In order to observe the morphological and paleopathological characteristics of the examined material, anthropometrics, computed tomography, spectrometry and microscopic methods were incorporated. Microscopic analysis revealed the presence of the extensive inflammatory lesions. Analyses indicate tertiary stage of venereal syphilis as the most probable cause of the observed lesions. Concentration of arsenic (16.17±0.58 μg/g) in examined bone samples was about hundred times bigger than average arsenic concentration in bones reported in other studies. Advanced stage of observed lesions along with high arsenic level may suggest long-lasting palliative care and usage of arsenic compound in therapeutic treatment of this chronic disease.
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http://dx.doi.org/10.1515/biol-2019-0048DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7874782PMC
January 2019

Diagnodent and VistaCam may be unsuitable for the evaluation of dental caries in archeological teeth.

Am J Phys Anthropol 2019 04 29;168(4):797-808. Epub 2019 Jan 29.

Department of Conservative Dentistry and Pedodontics, Wroclaw Medical University, Wroclaw, Poland.

Objectives: The aim of the study was to compare the usefulness of fluorescence-based caries detection systems (Diagnodent and VistaCam) for the assessment of carious lesions on archeological molars.

Materials And Methods: The study material consisted of teeth from the Cemetery of St. Mary Magdalene (Cmentarz św. Marii Magdaleny) in Wrocław, Poland. A sample of 178 permanent molars from 38 skulls were examined. Five surfaces of teeth (occlusal, mesial, distal, buccal, and lingual) were assessed on either basically cleaned or sandblasted teeth. Six diagnostic methods were used to detect carious lesions: the visual classification of the International Caries Detection and Assessment System (ICDAS II), fluorescent methods (Diagnodent and VistaCam), X-ray, cone beam computed tomography and histological sections. The sensitivity and specificity of the methods were determined using receiver operating characteristic (ROC) curves and the correlation between the severity of dental caries and the readouts obtained with each method.

Results: In most cases, Diagnodent and VistaCam yielded unsatisfactory specificity and sensitivity values. The area under curve (AUC) values in ROC curves for Diagnodent and Vistacam were lower than the AUC values obtained for the ICDAS II visual classification.

Conclusions: According to our results, in the case of archeological teeth, neither Diagnodent nor VistaCam can be regarded as a better diagnostic method than the ICDAS II visual classification of caries.
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http://dx.doi.org/10.1002/ajpa.23785DOI Listing
April 2019

The expression of IL10RA in colorectal cancer and its correlation with the proliferation index and the clinical stage of the disease.

Cytokine 2018 10 3;110:116-125. Epub 2018 May 3.

Department of Human Morphology and Embryology, Wroclaw Medical University, Wroclaw, Poland.

Introduction: Despite the widely described role of IL10 in immune response regulation during carcinogenesis, there is no established model describing the role of its receptor. The aim of this study is to elucidate the relationship between the subunit alpha of IL10 receptor (IL10RA) in the pathogenesis of colorectal cancer (CRC).

Methods: The study was conducted on archived paraffin blocks of 125 CRC patients, from which tissue microarrays (TMA) were made. These were subsequently used for immunohistochemistry to assess the expression of IL10RA, IL10, phosphorylated STAT3 (pSTAT3) and the Ki67 proliferation index. The intensity of both reactions was assessed by independent researchers using two approaches: digital image analysis and the Remmele and Stegner score (IRS). To assess the possible correlations between the two investigated markers and the clinical stage of CRC, the Pearson correlation coefficient was calculated. The expression of aforementioned proteins was assessed in tumor samples, healthy surgical margins and healthy control samples, obtained from cadavers during autopsy from the Department of Forensic Medicine. Statistical analysis was conducted using Statistica ver. 13.05 software.

Results: The final analysis included 105 CRC patients with complete clinical and pathological data, for whom the expressions of IL10RA, IL10, pSTAT3 and Ki67 were assessed using two independent methods. There was a positive correlation between the IL10RA expression and Ki-67 proliferation index (R = 0.63, p < 0.001) and a negative correlation between the IL10RA expression and the clinical stage of CRC (R = -0.21, p = 0.022). IL10RA correlated positively with pSTAT3 and IL10 in neoplastic tissue and tumor margin (with p < 0.01 for all correlations). We also observed a significantly higher expression of IL10RA in healthy surgical margins when compared to the actual tumor (p = 0.023, the paired t-test). The expression of IL10 was significantly higher in tumors than in healthy intestinal endothelium from control group.

Conclusions: The correlations between the expression of IL10RA and the proliferation index or the clinical stage of CRC seem to confirm the importance of IL10RA in the pathogenesis of CRC. The higher expression of IL10RA in healthy surgical margins than in the tumor itself may suggest that IL10RA plays a role in regulating immune response to the neoplasm.
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http://dx.doi.org/10.1016/j.cyto.2018.04.030DOI Listing
October 2018

Association Between Interleukin-10 Receptors and the CD45-Immunophenotype of Central Nervous System Tumors: A Preliminary Study.

Anticancer Res 2017 10;37(10):5777-5783

Department of Histology and Embryology, Wroclaw Medical University, Wroclaw, Poland.

Background/aim: One of the current hypotheses assumes that brain tumors exert an immunosuppressive influence on the surrounding cellular environment. Interleukin-10 (IL-10) is one of the immunosuppressive cytokines modifying the biological activity of cancer. The aim of this study was to assess the expression of IL10R in CD45 cells within primary brain tumors and metastases and establish its association with tumor basic immunophenotype.

Patients And Methods: Tissue samples were obtained intraoperatively during surgeries of 32 patients suffering from meningiomas (n=9), gliomas (n=12) and metastatic tumors (n=11). Expression was assessed with flow cytometry and immunohistochemical reactions.

Results: Expression of IL10R subunits within the leukocyte population (CD45 cells) was significantly higher in primary tumors than in metastases.

Conclusion: To the best of our knowledge, this is the first study describing a correlation between the IL10R expression on leukocytes and histological types of brain tumors.
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http://dx.doi.org/10.21873/anticanres.12019DOI Listing
October 2017

Association Between Interleukin-10 Receptors and the CD45-Immunophenotype of Central Nervous System Tumors: A Preliminary Study.

Anticancer Res 2017 10;37(10):5777-5783

Department of Histology and Embryology, Wroclaw Medical University, Wroclaw, Poland.

Background/aim: One of the current hypotheses assumes that brain tumors exert an immunosuppressive influence on the surrounding cellular environment. Interleukin-10 (IL-10) is one of the immunosuppressive cytokines modifying the biological activity of cancer. The aim of this study was to assess the expression of IL10R in CD45 cells within primary brain tumors and metastases and establish its association with tumor basic immunophenotype.

Patients And Methods: Tissue samples were obtained intraoperatively during surgeries of 32 patients suffering from meningiomas (n=9), gliomas (n=12) and metastatic tumors (n=11). Expression was assessed with flow cytometry and immunohistochemical reactions.

Results: Expression of IL10R subunits within the leukocyte population (CD45 cells) was significantly higher in primary tumors than in metastases.

Conclusion: To the best of our knowledge, this is the first study describing a correlation between the IL10R expression on leukocytes and histological types of brain tumors.
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http://dx.doi.org/10.21873/anticanres.12019DOI Listing
October 2017