Publications by authors named "Miao He"

726 Publications

The tectonigral pathway regulates appetitive locomotion in predatory hunting in mice.

Nat Commun 2021 07 20;12(1):4409. Epub 2021 Jul 20.

National Institute of Biological Sciences, Beijing, China.

Appetitive locomotion is essential for animals to approach rewards, such as food and prey. The neuronal circuitry controlling appetitive locomotion is unclear. In a goal-directed behavior-predatory hunting, we show an excitatory brain circuit from the superior colliculus (SC) to the substantia nigra pars compacta (SNc) to enhance appetitive locomotion in mice. This tectonigral pathway transmits locomotion-speed signals to dopamine neurons and triggers dopamine release in the dorsal striatum. Synaptic inactivation of this pathway impairs appetitive locomotion but not defensive locomotion. Conversely, activation of this pathway increases the speed and frequency of approach during predatory hunting, an effect that depends on the activities of SNc dopamine neurons. Together, these data reveal that the SC regulates locomotion-speed signals to SNc dopamine neurons to enhance appetitive locomotion in mice.
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http://dx.doi.org/10.1038/s41467-021-24696-3DOI Listing
July 2021

Effect of Fiber on Rheological Properties and Flow Behavior of Polymer Completion Fluids.

ACS Omega 2021 Jul 1;6(27):17136-17148. Epub 2021 Jul 1.

School of Petroleum Engineering, Yangtze University, Wuhan 430100, China.

The application of fiber in the completion fluid can improve the rheological properties of the completion fluid and the plugging quality of the production layer by the completion fluid and reduce the damage of the filtrate to the reservoir formation. However, there are few studies on the influence of fibers on the rheological properties of completion fluids and the flow behavior in pores. In this paper, plant fiber, mineral fiber, and synthetic fiber are discussed. Carbon fiber, bamboo fiber, polypropylene fiber, and polyester fiber are selected as research objects. The dependence of the rheological property of polymer solution on fiber type, fiber concentration, temperature, and shear rate is evaluated. The evaluation is carried out by observing the microscopic state of the fiber through a microscope and a scanning electron microscope, testing the rheological property parameters of the fiber with an OFITE 900 rheological tester, and fitting with the Herschel-Bulkley model. The results show that polypropylene fiber and carbon fiber have the best dispersion in polymer solution. The higher the fiber content, the greater the influence of fiber on the rheological properties of the solution. Compared with the other three fibers, carbon fiber has the greatest influence on the rheological properties of polymer solution. When the temperature is lower than 70 °C, the influence of the fiber on the rheological properties of the solution is not affected by the temperature. When the temperature exceeds 70 °C, the carbon fiber and polypropylene fiber are affected by the temperature, and the viscosity of the polymer solution is increased. The flow behavior of fiber suspensions in pores varies with the flow factor . Carbon fiber suspensions are most conducive to the transition of polymer solution to plate laminar flow, which can improve the bearing capacity of plugging materials.
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http://dx.doi.org/10.1021/acsomega.0c05346DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8280643PMC
July 2021

Kinetics of solid-liquid interface motion in molecular dynamics and phase-field models: crystallization of chromium and silicon.

Philos Trans A Math Phys Eng Sci 2021 Sep 19;379(2205):20200320. Epub 2021 Jul 19.

Otto Schott Institute of Materials Research, Physics-Astronomy Faculty, Friedrich Schiller University Jena, 07743 Jena, Germany.

The results of molecular dynamics (MD) simulations of the crystallization process in one-component materials and solid solution alloys reveal a complex temperature dependence of the velocity of the crystal-liquid interface featuring an increase up to a maximum at 10-30% undercooling below the equilibrium melting temperature followed by a gradual decrease of the velocity at deeper levels of undercooling. At the qualitative level, such non-monotonous behaviour of the crystallization front velocity is consistent with the diffusion-controlled crystallization process described by the Wilson-Frenkel model, where the almost linear increase of the interface velocity in the vicinity of melting temperature is defined by the growth of the thermodynamic driving force for the phase transformation, while the decrease in atomic mobility with further increase of the undercooling drives the velocity through the maximum and into a gradual decrease at lower temperatures. At the quantitative level, however, the diffusional model fails to describe the results of MD simulations in the whole range of temperatures with a single set of parameters for some of the model materials. The limited ability of the existing theoretical models to adequately describe the MD results is illustrated in the present work for two materials, chromium and silicon. It is also demonstrated that the MD results can be well described by the solution following from the hodograph equation, previously found from the kinetic phase-field model (kinetic PFM) in the sharp interface limit. The ability of the hodograph equation to describe the predictions of MD simulation in the whole range of temperatures is related to the introduction of slow (phase field) and fast (gradient flow) variables into the original kinetic PFM from which the hodograph equation is obtained. The slow phase-field variable is responsible for the description of data at small undercoolings and the fast gradient flow variable accounts for local non-equilibrium effects at high undercoolings. The introduction of these two types of variables makes the solution of the hodograph equation sufficiently flexible for a reliable description of all nonlinearities of the kinetic curves predicted in MD simulations of Cr and Si. This article is part of the theme issue 'Transport phenomena in complex systems (part 1)'.
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http://dx.doi.org/10.1098/rsta.2020.0320DOI Listing
September 2021

Development of a Novel Prognostic Signature Based on Antigen Processing and Presentation in Patients with Breast Cancer.

Pathol Oncol Res 2021 1;27:600727. Epub 2021 Apr 1.

Department of Pharmacology, School of Pharmacy, China Medical University, Shenyang, China.

Complex antigen processing and presentation processes are involved in the development and progression of breast cancer (BC). A single biomarker is unlikely to adequately reflect the complex interplay between immune cells and cancer; however, there have been few attempts to find a robust antigen processing and presentation-related signature to predict the survival outcome of BC patients with respect to tumor immunology. Therefore, we aimed to develop an accurate gene signature based on immune-related genes for prognosis prediction of BC. Information on BC patients was obtained from The Cancer Genome Atlas. Gene set enrichment analysis was used to confirm the gene set related to antigen processing and presentation that contributed to BC. Cox proportional regression, multivariate Cox regression, and stratified analysis were used to identify the prognostic power of the gene signature. Differentially expressed mRNAs between high- and low-risk groups were determined by KEGG analysis. A three-gene signature comprising HSPA5 (heat shock protein family A member 5), PSME2 (proteasome activator subunit 2), and HLA-F (major histocompatibility complex, class I, F) was significantly associated with OS. HSPA5 and PSME2 were protective (hazard ratio (HR) < 1), and HLA-F was risky (HR > 1). Risk score, estrogen receptor (ER), progesterone receptor (PR) and PD-L1 were independent prognostic indicators. KIT and ACACB may have important roles in the mechanism by which the gene signature regulates prognosis of BC. The proposed three-gene signature is a promising biomarker for estimating survival outcomes in BC patients.
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http://dx.doi.org/10.3389/pore.2021.600727DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8262234PMC
April 2021

Identification of DNA-Repair-Related Five-Gene Signature to Predict Prognosis in Patients with Esophageal Cancer.

Pathol Oncol Res 2021 30;27:596899. Epub 2021 Mar 30.

Department of Pharmacology, School of Pharmacy, China Medical University, Shenyang, China.

Esophageal cancer (ESCA) is a leading cause of cancer-related mortality, with poor prognosis worldwide. DNA damage repair is one of the hallmarks of cancer. Loss of genomic integrity owing to inactivation of DNA repair genes can increase the risk of cancer progression and lead to poor prognosis. We aimed to identify a novel gene signature related to DNA repair to predict the prognosis of ESCA patients. Based on gene expression profiles of ESCA patients from The Cancer Genome Atlas and gene set enrichment analysis, 102 genes related to DNA repair were identified as candidates. After stepwise Cox regression analysis, we established a five-gene prognostic model comprising DGCR8, POM121, TAF9, UPF3B, and BCAP31. Kaplan-Meier survival analysis confirmed a strong correlation between the prognostic model and survival. Moreover, we verified the clinical value of the prognostic signature under the influence of different clinical parameters. We found that small-molecule drugs (trametinib, selumetinib, and refametinib) could help to improve patient survival. In summary, our study provides a novel and promising prognostic signature based on DNA-repair-related genes to predict survival of patients with ESCA. Systematic data mining provides a theoretical basis for further exploring the molecular pathogenesis of ESCA and identifying therapeutic targets.
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http://dx.doi.org/10.3389/pore.2021.596899DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8262199PMC
March 2021

Non-Structural Protein 5 of Zika Virus Interacts with p53 in Human Neural Progenitor Cells and Induces p53-Mediated Apoptosis.

Virol Sin 2021 Jul 5. Epub 2021 Jul 5.

MOE Key Laboratory of Tropical Disease Control, Centre for Infection and Immunity Study (CIIS), School of Medicine, Sun Yat-Sen University, Shenzhen, 518197, China.

Zika virus (ZIKV) infection could disrupt neurogenesis and cause microcephaly in neonates by targeting neural progenitor cells (NPCs). The tumor suppressor p53-mediated cell cycle arrest and apoptotic cell death have been suggested to be activated upon ZIKV infection, yet the detailed mechanism is not well understood. In the present study, we investigated the effects of ZIKV-encoded proteins in the activation of p53 signaling pathway and found that, among the ten viral proteins, the nonstructural protein 5 (NS5) of ZIKV most significantly activated the transcription of p53 target genes. Using the immunoprecipitation-coupled mass spectrometry approach, we identified that ZIKV-NS5 interacted with p53 protein. The NS5-p53 interaction was further confirmed by co-immunoprecipitation and GST pull-down assays. In addition, the MTase domain of NS5 and the C-terminal domain of p53 were mapped to be responsible for the interaction between these two proteins. We further showed that ZIKV-NS5 was colocalized with p53 and increased its protein level in the nuclei and able to prolong the half-life of p53. Furthermore, lentivirus-mediated expression of ZIKV-NS5 in hNPCs led to an apparent cell death phenotype. ZIKV-NS5 promoted the cleavage of PARP1 and significantly increased the cell apoptosis of hNPCs. Taken together, these findings revealed that ZIKV-NS5 is a previously undiscovered regulator of p53-mediated apoptosis in hNPCs, which may contribute to the ZIKV-caused abnormal neurodevelopment.
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http://dx.doi.org/10.1007/s12250-021-00422-7DOI Listing
July 2021

Role of lymphatic endothelial cells in the tumor microenvironment-a narrative review of recent advances.

Transl Lung Cancer Res 2021 May;10(5):2252-2277

Department of Thoracic Surgery, China State Key Laboratory of Respiratory Disease and National Clinical Research Center for Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.

Background: As lymphatic vessel is a major route for solid tumor metastasis, they are considered an essential part of tumor drainage conduits. Apart from forming the walls of lymphatic vessels, lymphatic endothelial cells (LECs) have been found to play multiple other roles in the tumor microenvironment, calling for a more in-depth review. We hope that this review may help researchers gain a detailed understanding of this fast-developing field and shed some light upon future research.

Methods: To achieve an informative review of recent advance, we carefully searched the Medline database for English literature that are openly published from the January 1995 to December 2020 and covered the topic of LEC or lymphangiogenesis in tumor progression and therapies. Two different authors independently examined the literature abstracts to exclude possible unqualified ones, and 310 papers with full texts were finally retrieved.

Results: In this paper, we discussed the structural and molecular basis of tumor-associated LECs, together with their roles in tumor metastasis and drug therapy. We then focused on their impacts on tumor cells, tumor stroma, and anti-tumor immunity, and the molecular and cellular mechanisms involved. Special emphasis on lung cancer and possible therapeutic targets based on LECs were also discussed.

Conclusions: LECs can play a much more complex role than simply forming conduits for tumor cell dissemination. Therapies targeting tumor-associated lymphatics for lung cancer and other tumors are promising, but more research is needed to clarify the mechanisms involved.
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http://dx.doi.org/10.21037/tlcr-21-40DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8182726PMC
May 2021

NF-κB-activated SPRY4-IT1 promotes cancer cell metastasis by downregulating TCEB1 mRNA via Staufen1-mediated mRNA decay.

Oncogene 2021 Jun 23. Epub 2021 Jun 23.

Department of Pharmacology, School of Pharmacy, China Medical University, No.77 Puhe Road, Shenyang North New Area, Shenyang City, 110122, Liaoning, China.

Previous study demonstrated that most long non-coding RNAs (lncRNAs) function as competing endogenous RNAs or molecular sponges to negatively modulate miRNA and regulate tumor development. However, the molecular mechanisms of lncRNAs in cancer are not fully understood. Our study describes the role of the lncRNA SPRY4 intronic transcript 1 (SPRY4-IT1) in cancer metastasis by mechanisms related to Staufen1 (STAU1)-mediated mRNA decay (SMD). Briefly, we found that, high SPRY4-IT1 expression was associated with aggressiveness and poor outcome in human colorectal, breast and ovarian cancer tissues. In addition, functional assays revealed that SPRY4-IT1 significantly promoted colorectal, breast and ovarian cancer metastasis in vitro and in vivo. Mechanistically, microarray analyses identified several differentially-expressed genes upon SPRY4-IT1 overexpression in HCT 116 colorectal cancer cells. Among them, the 3'-UTR of transcription elongation factor B subunit 1 (TCEB1) mRNA can base-pair with the Alu element in the 3'-UTR of SPRY4-IT1. Moreover, SPRY4-IT1 was found to bind STAU1, promote STAU1 recruitment to the 3'-UTR of TCEB1 mRNA, and affect TCEB1 mRNA stability and expression, resulting in hypoxia-inducible factor 1α (HIF-1α) upregulation, and thereby affecting cancer cell metastasis. In addition, STAU1 depletion abrogated TCEB1 SMD and alleviated the pro-metastatic effect of SPRY4-IT1 overexpression. Significantly, we revealed that SPRY4-IT1 is also transactivated by NF-κB/p65, which activates SPRY4-IT1 to inhibit TCEB1 expression, and subsequently upregulate HIF-1α. In conclusion, our results highlight a novel mechanism of cytoplasmic lncRNA SPRY4-IT1 in which SPRY4-IT1 affecting TCEB1 mRNA stability via STAU1-mediated degradation during cancer metastasis.
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http://dx.doi.org/10.1038/s41388-021-01900-8DOI Listing
June 2021

Reappraisal of anticancer nanomedicine design criteria in three types of preclinical cancer models for better clinical translation.

Biomaterials 2021 Jun 3;275:120910. Epub 2021 Jun 3.

Department of Pharmaceutical Sciences, College of Pharmacy, University of Michigan, 1600 Huron Parkway, North Campus Research Complex, Building 520, Ann Arbor, MI, 48109, USA. Electronic address:

Anticancer nanomedicines are designed to improve anticancer efficacy by increasing drug accumulation in tumors through enhanced permeability retention (EPR) effect, and to reduce toxicity by decreasing drug accumulation in normal organs through long systemic circulation. However, the inconsistent efficacy/safety of nanomedicines in cancer patients versus preclinical cancer models have provoked debate for nanomedicine design criteria. In this study, we investigate nanomedicine design criteria in three types of preclinical cancer models using five clinically used nanomedicines, which identifies the factors for better clinical translations of their observed clinical efficacy/safety compared to free drug or clinical micelle formulation. When those nanomedicines were compared with drug solution or clinical micelle formulation in breast tumors, long and short-circulating nanomedicines did not enhance tumor accumulation by EPR effect in transgenic spontaneous breast cancer model regardless of their size or composition, although they improved tumor accumulations in subcutaneous and orthotopic breast cancer models. However, when tumors were compared to normal breast tissue, nanomedicines, drug solution and clinical micelle formulation showed enhanced tumor accumulation regardless of the breast cancer models. In addition, long-circulating nanomedicines did not further increase tumor accumulation in transgenic mouse spontaneous breast cancer nor universally decrease drug accumulations in normal organs; they decreased or increased accumulation in different organs, potentially changing the clinical efficacy/safety. In contrast, short-circulating nanomedicines decreased blood concentration and altered drug distribution in normal organs, which are correlated with their clinical efficacy/safety. A reappraisal of current nanomedicine design criteria is needed to ensure consistent clinical translation for improvement of their clinical efficacy/safety in cancer patients.
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http://dx.doi.org/10.1016/j.biomaterials.2021.120910DOI Listing
June 2021

Bi-allelic variants in the ER quality-control mannosidase gene EDEM3 cause a congenital disorder of glycosylation.

Am J Hum Genet 2021 Jul 17;108(7):1342-1349. Epub 2021 Jun 17.

Department of Human Genetics, Donders Institute for Brain, Cognition, and Behavior, Radboud University Medical Center, 6500 HB Nijmegen, the Netherlands. Electronic address:

EDEM3 encodes a protein that converts ManGlcNAc isomer B to ManGlcNAc. It is involved in the endoplasmic reticulum-associated degradation pathway, responsible for the recognition of misfolded proteins that will be targeted and translocated to the cytosol and degraded by the proteasome. In this study, through a combination of exome sequencing and gene matching, we have identified seven independent families with 11 individuals with bi-allelic protein-truncating variants and one individual with a compound heterozygous missense variant in EDEM3. The affected individuals present with an inherited congenital disorder of glycosylation (CDG) consisting of neurodevelopmental delay and variable facial dysmorphisms. Experiments in human fibroblast cell lines, human plasma, and mouse plasma and brain tissue demonstrated decreased trimming of ManGlcNAc isomer B to ManGlcNAc, consistent with loss of EDEM3 enzymatic activity. In human cells, ManGlcNAc to ManGlcNAc conversion is also diminished with an increase of GlcManGlcNAc. Furthermore, analysis of the unfolded protein response showed a reduced increase in EIF2AK3 (PERK) expression upon stimulation with tunicamycin as compared to controls, suggesting an impaired unfolded protein response. The aberrant plasma N-glycan profile provides a quick, clinically available test for validating variants of uncertain significance that may be identified by molecular genetic testing. We propose to call this deficiency EDEM3-CDG.
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http://dx.doi.org/10.1016/j.ajhg.2021.05.010DOI Listing
July 2021

Elastic Net Models Based on DNA Copy Number Variations Predicts Clinical Features, Expression Signatures, and Mutations in Lung Adenocarcinoma.

Front Genet 2021 31;12:668040. Epub 2021 May 31.

Department of Oncology, The First Affiliated Hospital, Gannan Medical University, Ganzhou, China.

In the precision medicine of lung adenocarcinoma, the identification and prediction of tumor phenotypes for specific biomolecular events are still not studied in depth. Various earlier researches sheds light on the close correlation between genetic expression signatures and DNA copy number variations (CNVs), for which analysis of CNVs provides valuable information about molecular and phenotypic changes in tumorigenesis. In this study, we propose a comprehensive analysis combining genome-wide association analysis and an Elastic Net Regression predictive model, focus on predicting the levels of many gene expression signatures in lung adenocarcinoma, based upon DNA copy number features alone. Additionally, we predicted many other key phenotypes, including clinical features (pathological stage), gene mutations, and protein expressions. These Elastic Net prediction methods can also be applied to other gene sets, thereby facilitating their use as biomarkers in monitoring therapy.
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http://dx.doi.org/10.3389/fgene.2021.668040DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8202527PMC
May 2021

Sex-specific gene expression in the blood of four primates.

Genomics 2021 Jul 8;113(4):2605-2613. Epub 2021 Jun 8.

Key Laboratory of Bioresources and Eco-Environment (Ministry of Education), College of Life Sciences, Sichuan University, Chengdu 610065, Sichuan, China. Electronic address:

Blood is an important non-reproductive tissue, but little is known about the sex-specific gene expressions in the blood. Therefore, we investigated sex-specific gene expression differences in the blood tissues of four primates, rhesus macaques (Macaca mulatta), Tibetan macaques (M. thibetana), yellow baboons (Papio cynocephalus), and humans. We identified seven sex-specific differentially expressed genes (SDEGs) in each non-human primate and 31 SDEGs in humans. The four primates had only one common SDEG, MAP7D2. In humans, immune-related SDEGs were identified as up-regulated, but also down-regulated in females. We also found that most of the X-Y gene pairs had similar expression levels between species, except pair EIF1AY/EIF1AX. The expression level of X-Y gene pairs of rhesus and Tibetan macaques showed no significant differential expression levels, while humans had six significant XY-biased and three XX-biased X-Y gene pairs. Our observed sex differences in blood should increase understanding of sex differences in primate blood tissue.
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http://dx.doi.org/10.1016/j.ygeno.2021.06.007DOI Listing
July 2021

Panobinostat penetrates the blood-brain barrier and achieves effective brain concentrations in a murine model.

Cancer Chemother Pharmacol 2021 Jun 11. Epub 2021 Jun 11.

Department of Clinical Pharmacy and Pharmacy Services, Inpatient Hematology, University of Michigan College of Pharmacy, 1540 E. Hospital Dr., CW 7-251B, Ann Arbor, MI, 48109-5008, US.

Purpose: Panobinostat, an orally bioavailable pan-HDAC inhibitor, has demonstrated potent activity in multiple malignancies, including pediatric brain tumors such as DIPG, with increased activity against H3K27M mutant cell lines. Given limited evidence regarding the CNS penetration of panobinostat, we sought to characterize its BBB penetration in a murine model.

Methods: Panobinostat 15 mg/kg was administered IV to 12 CD-1 female mice. At specified time points, mice were euthanized, blood samples were collected, and brains were removed. LC-MS was performed to quantify panobinostat concentrations. C and AUC were estimated and correlated with previously published pharmacokinetic analyses and reports of IC-50 values in DIPG cell lines.

Results: Mean panobinostat plasma concentrations (ng/mL) were 27.3 ± 2.5 at 1 h, 7.56 ± 1.8 at 2 h, 1.48 ± 0.56 at 4 h, and 2.33 ± 1.18 at 7 h. Mean panobinostat brain concentrations (ng/g) were 60.5 ± 6.1 at 1 h, 42.9 ± 5.4 at 2 h, 33.2 ± 6.1 at 4 h, and 28.1 ± 4.3 at 7 h. Brain-to-plasma ratio at 1 h was 2.22 and the brain to plasma AUC ratio was 2.63. Based on the published human pharmacokinetic data, the anticipated C in humans is expected to be significantly higher than the IC-50 identified in DIPG models.

Conclusion: It is expected that panobinostat would be effective in CNS tumors where the IC-50 is in the low nanomolar range. Thus, our data demonstrate panobinostat crosses the BBB and achieves concentrations above the IC-50 for DIPG and other brain tumors and should be explored further for clinical efficacy.
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http://dx.doi.org/10.1007/s00280-021-04313-2DOI Listing
June 2021

Synergistic Effect of Polyaspartate and Polyethylene Glycol on Lubrication Performance of the Water-Based Drilling Mud.

ACS Omega 2021 Jun 21;6(21):13817-13830. Epub 2021 May 21.

JiahuaKeji Co., Let., Jingzhou 434000, China.

The poor lubrication performance of water-based drilling mud hinders its application in the drilling process of extended, reach horizontal wells. To overcome this shortcoming, polyaspartate (PA) and poly(ethylene glycol) (PEG) were used to improve the lubrication performance of the water-based drilling mud. The conventional performances, lubrication performance, and micro-image of antiwear steel balls of the modified water-based drilling mud were analyzed. The results show that the coefficient of friction (COF) of the water-based drilling mud mixed with 10% PA and 5% PEG was the lowest, reaching 0.094, the reduction rate of COF was 63.1%, and the drilling mud cake stuck factor was also the lowest. The addition of PA and PEG had no effect on the rheological properties of water-based drilling mud and also can significantly reduce the filtrate volume; the reduction rate of the filtrate volume reached 43.5%. All of these result from the synergistic effect of PA and PEG; they are adsorbed on the metal surface and the mud cake to form a lubricating film. At the same time, the lubricants also changed the appearance of the solid particles in the mud cake, which reduced the friction between the mud cake and the drilling tool. Moreover, effects of the influence of drilling mud properties on lubricants (alkali metal ions, pH, temperature, and drilling mud density) were examined. The water-based drilling fluid with the synergistic effect of PEG and PA shows similar lubrication performance as the oil-based drilling fluid and can meet the technical requirements of corresponding horizontal wells.
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http://dx.doi.org/10.1021/acsomega.1c01361DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8173554PMC
June 2021

Surface atomic modulation of CoP bifunctional catalyst for high performance Li-O battery enabled by high-index (211) facets.

J Colloid Interface Sci 2021 May 20;601:114-123. Epub 2021 May 20.

College of Materials and Chemistry & Chemical Engineering, Chengdu University of Technology, 1#, Dongsanlu, Erxianqiao, Chengdu 610059, Sichuan, PR China. Electronic address:

The rational design of the surface structure and morphology characteristics of the catalyst at atomic level are the key to improve the oxygen evolution reaction (OER) and oxygen reduction reaction (ORR) in lithium-oxygen (Li-O) battery. Here a series of cobalt phosphide (CoP) electrocatalysts with a variety of index facets are successfully prepared including concave polyhedrons CoP exposing with (211) crystal planes (CoP CPHs) spherical nanoparticles CoP exposed with (011) crystal planes and polyhedron particles CoP exposing with (011) and (111) crystal planes. The results show that CoP CPHs based Li-O battery presents a large discharge capacity of 33743 mA h g at current density of 50 mA g and a remarkable long cycle life of up to 950 h. The experimental results demonstrates that the CoP CPHs electrode exposing with high-index (211) facets based Li-O battery exhibits an extremely low overpotential (0.67 V) ultrahigh specific capacity (33743 mAh g) and remarkable long-term stability of up to 950 h. Most importantly density functional theory (DFT) calculations demonstrate the excellent electrocatalytic activity of high-index (211) facets as compared to the low-index (011) and (111) planes are because of the existence of large density of atomic steps edge ledge sites and kinks which supply a wide space for breaking chemical bonds and increasing the reaction activity for oxygen electrode.
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http://dx.doi.org/10.1016/j.jcis.2021.05.097DOI Listing
May 2021

Diagnostic and Therapeutic Potential of Exosomal MicroRNAs for Neurodegenerative Diseases.

Neural Plast 2021 16;2021:8884642. Epub 2021 May 16.

Department of Orthopaedics, Xiangya Hospital, Central South University, Changsha, 410008 Hunan, China.

Neurodegenerative disorders (NDs) are characterized by a gradual loss of neurons and functions that eventually leads to progressive neurological impairment. In view of the heavy burden on the healthcare system, efficient and reliable biomarkers for early diagnosis and therapeutic treatments to reverse the progression of NDs are in urgent need. There has been an increasing interest in using exosomal miRNAs as biomarkers or targeted therapies for neurological diseases recently. In this review, we overviewed the updated studies on exosomal miRNAs as biomarkers and potential therapeutic approaches in NDs, as well as their association with the pathophysiology of this group of disorders, especially Alzheimer's disease (AD), Parkinson's disease (PD), amyotrophic lateral sclerosis (ALS), and Huntington's disease (HD). The exosomal miRNAs that are commonly dysregulated across different NDs or are commonly used as therapeutic candidates were also identified and summarized. In summary, the feasibility of exosomal miRNAs as biomarkers and potential targeted therapy for NDs has been verified. However, due to the limitations of existing studies and the discrepancies across different studies, high quality laboratory and clinical investigations are still required.
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http://dx.doi.org/10.1155/2021/8884642DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8143892PMC
May 2021

Efficacy and safety of short-wave diathermy treatment for moderate COVID-19 patients: a prospective, double-blind, randomized controlled clinical study.

Eur J Phys Rehabil Med 2021 May 27. Epub 2021 May 27.

Department of Rehabilitation Medicine, Xijing Hospital, the Fourth Military Medical University, Xi'an, China -

Background: Millions of human beings have suffered in the epidemic of coronavirus disease 2019 (COVID-19), but until now the effective treatment methods have been limited.

Aim: This study aimed to evaluate the efficacy and safety of short-wave diathermy (SWD) treatment for moderate COVID-19 patients.

Design: A prospective, double-blind, randomized controlled clinical study.

Setting: Inpatients unit of a COVID-19 specialized hospital.

Population: Forty-two patients with moderate COVID-19 were randomly allocated at a 2:1 ratio to two groups: the SWD group and the control group.

Methods: Participants of the SWD group received SWD treatment, and participants of the control group received placebo SWD treatment for one session per day, 10 minutes per session, for no more than 14 days. Both groups were given standard care treatment. Primary outcome was the rate of clinical improvement according to a sevencategory ordinal scale. Secondary outcomes included the rate of computed tomography (CT) improvement and the rate of potential adverse events.

Results: Clinical improvement occurred in 92.6% of patients in the SWD group by day 14 compared with 69.2% of patients in the control group (P = 0.001). The Cox model indicated that the SWD group had a higher clinical improvement probability than the control group (Hazard Ratio, 3.045; 95% CI, 1.391-6.666; P = 0.005). Similarly, CT improvement occurred in 85.2 % of patients in the SWD group and 46.2 % of patients in the control group respectively by day 14 (P = 0.001). The Cox model indicated SWD group had a higher CT improvement probability than control group (Hazard Ratio, 3.720; 95% CI, 1.486-9.311; P = 0.005). There was no significant difference in adverse events between the SWD group and the control group (2 of 27 [7.4%] SWD vs. 1 of 13 [7.7%] control, P = 1.000), the most frequent of which were headache (1 of 27 [3.7%] SWD vs. 1 of 13 [7.7%] control patients) and dizziness (1 of 27 [3.7%] SWD vs. 0 of 13 [0%] control patients).

Conclusions: SWD is a valid and reliable adjuvant therapy with a favorable safety profile for moderate COVID-19 patients.

Clinical Rehabilitation Impact: Clinically relevant information is lacking regarding the efficacy and safety of SWD for patients with COVID-19. This study provides the first evidence that SWD is a promising adjuvant therapy for COVID-19.
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http://dx.doi.org/10.23736/S1973-9087.21.06892-1DOI Listing
May 2021

Spatio-temporal analysis of urban air pollutants throughout China during 2014-2019.

Air Qual Atmos Health 2021 May 14:1-14. Epub 2021 May 14.

Department of Environmental Health, School of Public Health, Key Laboratory of Environmental Health Damage Research and Assessment, China Medical University, Shenyang, 110122 Liaoning Province China.

Air pollution control has become the top priority of China's "green development" concept since 2013. The Chinese government has enacted a range of policies and statutes to control contaminant emissions and improve air quality. On the basis of the national air quality ground observation database, the spatial and temporal distribution of air quality index value (AQI), fine particulate matter (PM), coarse particles (PM), sulfur dioxide (SO), nitrogen dioxide (NO), carbon monoxide (CO), and ozone (O) were explored in 336 cities throughout China from 2014 to 2019. AQI and most pollutants (except O) decreased in concentrations from 2014 to 2019. In 2019, all cities except Henan reached the level 2 of the ambient air quality index, and six cities had a lower ambient air quality index and reached the level 1. Spatially, higher pollutant concentrations were concentrated in large city clusters, whereas the areas with high O concentration were found across the country. Furthermore, central heating was shown to have a negative impact on air quality. The observed AQI value, PM, PM, SO, NO, and CO concentrations were highest in north and northwest China and Henan province in central China. The correlations among pollutants suggest that the main sources of pollutants are fossil fuel combustion, industrial production, and motor vehicle emissions. The influence of meteorological factors on air quality, long-distance transportation, and the transformations of pollutants should be explored in future research.

Supplementary Information: The online version contains supplementary material available at 10.1007/s11869-021-01043-5.
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http://dx.doi.org/10.1007/s11869-021-01043-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121134PMC
May 2021

The effects of posterior cruciate ligament rupture on the biomechanical and histological characteristics of the medial collateral ligament: an animal study.

J Orthop Surg Res 2021 May 21;16(1):330. Epub 2021 May 21.

Department of Orthopaedics, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha, 410008, Hunan, China.

Background: To investigate the effect of complete rupture of the posterior cruciate ligament (PCL) on the biomechanics and histology of the medial collateral ligament (MCL).

Materials And Methods: Seventy-two male rabbits were randomly divided into two groups: the ruptured group was treated with complete PCL amputation, while the intact group was only subjected to PCL exposure without amputation. Eighteen rabbits were randomly sacrificed at 8, 16, 24, and 40 weeks after the operation, and their specimens were processed for mechanical tensile testing, nano-indentation experiments, hematoxylin-eosin (HE) staining, and picrosirius-polarization staining.

Results: There was no significant difference in the length and maximum displacement of the MCL between the ruptured group and the intact group at each time point. The maximum load of the ruptured group was significantly smaller than that of the intact group at 40 W. The elastic modulus and micro-hardness of the ruptured group increased significantly at 24 W and decreased significantly at 40 W. At 16 W and 24 W after PCL rupture, the number of type I collagen fibers and type III collagen fibers in the MCL of the ruptured group was significantly increased compared with that of the intact group. While the type I collagen fibers of the ruptured group were significantly decreased compared with the intact group at 40 W, there was no significant difference in type III collagen fibers between the ruptured group and the intact group.

Conclusion: PCL rupture has no significant effect on the mechanical and histological properties of MCL in a short period of time under physiological loading, but the histological and mechanical properties of MCL decrease with time.
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http://dx.doi.org/10.1186/s13018-021-02443-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8139104PMC
May 2021

LncRNA PCNAP1 Promotes Hepatoma Cell Proliferation through Targeting miR-340-5p and is Associated with Patient Survival.

J Oncol 2021 28;2021:6627173. Epub 2021 Apr 28.

Department of Critical Care Medicine, Chongqing Traditional Chinese Medicine Hospital, Chongqing 400011, China.

Hepatocellular carcinoma (HCC) is one of the most common malignancies and causes poor outcome. Dysregulation of long noncoding RNA (lncRNA) is involved in HCC. Upregulation of the lncRNA PCNAP1 has been reported to promote HBV-infectious HCC growth, but its clinical significance and underlying mechanisms in HCC development remain unclear. Here, we report that PCNAP1 expression is increased in both HBV-infectious and noninfectious HCC tissues compared with matched normal tissues, and its upregulation correlates with poor survival rates of HCC patients. Furthermore, we found that PCNAP1 promotes HCC cell proliferation through acting as a competitive endogenous RNA (ceRNA) to sponge miR-340-5p, which has been reported to directly inhibit ATF7 expression in HCC cells. Moreover, the PCNAP1/miR-340-5p/ATF7 signaling associates with the poor survival rates of HCC patients. Collectively, our findings suggest that the PCNAP1/miR-340-5p/ATF7 signaling may be a potential biomarker for the prognosis of HCC patients and a potential therapeutic target for HCC.
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http://dx.doi.org/10.1155/2021/6627173DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8100385PMC
April 2021

Discovery of first-in-class inhibitors of ASH1L histone methyltransferase with anti-leukemic activity.

Nat Commun 2021 05 14;12(1):2792. Epub 2021 May 14.

Department of Pathology, University of Michigan, Ann Arbor, MI, USA.

ASH1L histone methyltransferase plays a crucial role in the pathogenesis of different diseases, including acute leukemia. While ASH1L represents an attractive drug target, developing ASH1L inhibitors is challenging, as the catalytic SET domain adapts an inactive conformation with autoinhibitory loop blocking the access to the active site. Here, by applying fragment-based screening followed by medicinal chemistry and a structure-based design, we developed first-in-class small molecule inhibitors of the ASH1L SET domain. The crystal structures of ASH1L-inhibitor complexes reveal compound binding to the autoinhibitory loop region in the SET domain. When tested in MLL leukemia models, our lead compound, AS-99, blocks cell proliferation, induces apoptosis and differentiation, downregulates MLL fusion target genes, and reduces the leukemia burden in vivo. This work validates the ASH1L SET domain as a druggable target and provides a chemical probe to further study the biological functions of ASH1L as well as to develop therapeutic agents.
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http://dx.doi.org/10.1038/s41467-021-23152-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121805PMC
May 2021

Self-Templated Formation of Fluffy Graphene-Wrapped NiP Hollow Spheres for Li-Ion Battery Anodes with High Cycling Stability.

ACS Appl Mater Interfaces 2021 May 14;13(20):23714-23723. Epub 2021 May 14.

Department of Energy Science and Engineering, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Daegu 42988, Republic of Korea.

Transition-metal phosphides have gained great importance in the field of energy conversion and storage such as electrochemical water splitting, fuel cells, and Li-ion batteries. In this study, a rationally designed novel fluffy graphene (FG)-wrapped monophasic NiP ([email protected]) is in-situ-synthesized using a chemical vapor deposition method as a Li-ion battery anode material. The porous and hollow structure of NiP core is greatly helpful for lithium-ion diffusion, and at the same time, the cilia-like graphene nanosheet shell provides an electron-conducting layer and stabilizes the solid electrolyte interface formed on the NiP surface. The [email protected] sample shows a high reversible capacity of 739 mAh g after 300 cycles at a specific current density of 500 mA g. The high capacity, superior cycling stability, and improved rate capability of [email protected] are ascribed to its unique hierarchical structure. Moreover, the present efficient fabrication methodology of [email protected] has potential to be developed as a general method for the synthesis of other transition-metal phosphides.
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http://dx.doi.org/10.1021/acsami.1c03696DOI Listing
May 2021

Kangfuxin Liquid Ameliorates Dextran Sulfate Sodium (DSS)-Induced Acute Ulcerative Colitis in Mice by Modulating Immune Response and Suppressing Inflammation.

Med Sci Monit Basic Res 2021 May 11;27:e930887. Epub 2021 May 11.

National-Local Joint Engineering Research Center of Entomoceutics, Dali, Yunnan, China (mainland).

BACKGROUND The aim of this study was to determine the effect of kangfuxin liquid (KFXL) on inflammatory response, and its underlying mechanism in treating acute ulcerative colitis (UC) in mice induced by dextran sulfate sodium (DSS). MATERIAL AND METHODS Mice were provided drinking water containing DSS (3%) for 7 days to induce acute enteritis. The mice were divided into 6 groups: a control group, a DSS-induced (vehicle) group, a sulfasalazine (SASP) group, and low-, medium-, and high-dose kangfuxin liquid groups. Disease activity index (DAI), colon mucosa damage index (CMDI), histopathological score (HS), and organ index were monitored daily. The levels of interleukin-1ß (IL-1ß), interleukin-10 (IL-10) in serum and interleukin-17 (IL-17) and epidermal growth factor (EGF) in colon tissue were assessed by enzyme-linked immunosorbent assay (ELISA). Flow cytometry was used to assess the changes of T lymphocyte subsets in spleens of mice to evaluate the therapeutic effect of drugs on acute UC in mice. RESULTS Different doses of kangfuxin liquid reduced the DAI, CMDI, and HS scores (P<0.01 or P<0.05) of acute UC mice, reduced the level of IL-1ß and IL-17 in serum, increased the expression of IL-10 in serum and EGF in colon tissue, increased the number of CD3⁺ T cells, and decreased the level of CD4⁺ T cells and the ratio of CD4⁺/CD8⁺. CONCLUSIONS Kangfuxin liquid has a therapeutic effect on DSS-induced acute UC in mice, and its mechanism of action may be associated with regulating immune function and reducing intestinal inflammatory response.
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May 2021

Cooking with biomass fuels increased the risk for cognitive impairment and cognitive decline among the oldest-old Chinese adults (2011-2018): A prospective cohort study.

Environ Int 2021 Oct 4;155:106593. Epub 2021 May 4.

Department of Environmental Health, School of Public Health, China Medical University, Shenyang 110122, China. Electronic address:

Backgrounds: While the pernicious effects of outdoor air pollution on cognitive ability have been previously examined, evidence regarding household air pollution is scarce.

Methods: Using data from the Chinese Longitudinal Healthy Longevity Survey, we explored the relationship between cooking with biomass fuel and cognitive impairment and cognitive decline using a Cox proportional hazards model. We further assessed the correlation of biomass fuels and cognitive score using a generalized estimating equation. Cognitive ability was measured based on the Chinese version of the Mini-Mental State Examination (MMSE) and cognitive impairment was defined as MMSE < 24 points and cognitive decline was defined as a reduction of MMSE ≥ 3 points. On follow-up, we investigated the effect of switch-cooking combustibles on cognitive ability.

Results: The mean (SD) age of 4161 participants was 81.7 (10.0) years old. The reported cooking with biomass fuels was correlated with an elevated risk of cognitive impairment (hazard ratio (HR): 1.19, 95% confidence interval (CI): 1.04, 1.37) and cognitive decline (HR: 1.18, 95% CI: 1.04, 1.33). Besides, cooking with biomass fuels was related to a decrease in cognitive score (β: -0.43, 95% CI: -0.73, -0.14). In comparison to persistent biomass fuel users, participants who reported changing their primary cooking fuels from biomass to clean fuels exhibited a reduced risk of cognitive impairment (HR: 0.68, 95% CI: 0.57, 0.82) and cognitive decline (HR: 0.66, 95% CI: 0.56, 0.76) and a higher cognitive score (β: 0.72, 95% CI: 0.17, 1.26). Cooking without ventilated cookstoves was associated with a higher risk of cognitive impairment (HR: 1.31, 95% CI: 1.10, 1.58) and cognitive decline (HR: 1.18, 95% CI: 1.02, 1.38), regardless of types of cooking fuels. Interaction and stratified analyses showed relatively poor cognitive ability in participants who engaged in irregular exercise or were not living with family members.

Conclusions: Cooking with biomass fuels was correlated with a higher risk of cognitive impairment and cognitive decline. Among the oldest-old population, this risk may, however, be lower for those changing their primary cooking fuels from biomass to clean fuels.
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http://dx.doi.org/10.1016/j.envint.2021.106593DOI Listing
October 2021

Mouse models of sarcopenia: classification and evaluation.

J Cachexia Sarcopenia Muscle 2021 Jun 5;12(3):538-554. Epub 2021 May 5.

Department of Orthopedics, Xiangya Hospital, Central South University, Changsha, Hunan, China.

Sarcopenia is a progressive and widespread skeletal muscle disease that is related to an increased possibility of adverse consequences such as falls, fractures, physical disabilities and death, and its risk increases with age. With the deepening of the understanding of sarcopenia, the disease has become a major clinical disease of the elderly and a key challenge of healthy ageing. However, the exact molecular mechanism of this disease is still unclear, and the selection of treatment strategies and the evaluation of its effect are not the same. Most importantly, the early symptoms of this disease are not obvious and are easy to ignore. In addition, the clinical manifestations of each patient are not exactly the same, which makes it difficult to effectively study the progression of sarcopenia. Therefore, it is necessary to develop and use animal models to understand the pathophysiology of sarcopenia and develop therapeutic strategies. This paper reviews the mouse models that can be used in the study of sarcopenia, including ageing models, genetically engineered models, hindlimb suspension models, chemical induction models, denervation models, and immobilization models; analyses their advantages and disadvantages and application scope; and finally summarizes the evaluation of sarcopenia in mouse models.
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http://dx.doi.org/10.1002/jcsm.12709DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8200444PMC
June 2021

[Cortical 5-hydroxytryptamine receptor 3A (Htr3a) positive inhibitory neurons: diversity in type and function].

Sheng Li Xue Bao 2021 Apr;73(2):295-305

Institutes of Brain Science, Fudan University, Shanghai 200032, China.

Cortical GABAergic inhibitory neurons are composed of three major classes, each expressing parvalbumin (PV), somatostatin (SOM) and 5-hydroxytryptamine receptor 3A (Htr3a), respectively. Htr3a inhibitory neurons are mainly derived from the caudal ganglionic eminence (CGE). This highly heterogeneous group of inhibitory neurons are comprised of many different subtypes with distinct molecular signatures, morphological and electrophysiological properties and connectivity patterns. In this review, we summarized recent research progress regarding cortical Htr3a inhibitory neurons, focusing on their molecular, morphological and electrophysiological diversity, and introduced some genetic mouse tools that were used to study Htr3a inhibitory neurons.
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April 2021

Cascade diversification directs generation of neuronal diversity in the hypothalamus.

Cell Stem Cell 2021 Apr 17. Epub 2021 Apr 17.

State Key Laboratory of Molecular Development Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100101, China; CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai 200031, China; Chinese Institute for Brain Research, Beijing 102206, China. Electronic address:

The hypothalamus contains an astounding heterogeneity of neurons that regulate endocrine, autonomic, and behavioral functions. However, its molecular developmental trajectory and origin of neuronal diversity remain unclear. Here, we profile the transcriptome of 43,261 cells derived from Rax hypothalamic neuroepithelium to map the developmental landscape of the mouse hypothalamus and trajectory of radial glial cells (RGCs), intermediate progenitor cells (IPCs), nascent neurons, and peptidergic neurons. We show that RGCs adopt a conserved strategy for multipotential differentiation but generate Ascl1 and Neurog2 IPCs. Ascl1 IPCs differ from their telencephalic counterpart by displaying fate bifurcation, and postmitotic nascent neurons resolve into multiple peptidergic neuronal subtypes. Clonal analysis further demonstrates that single RGCs can produce multiple neuronal subtypes. Our study reveals that multiple cell types along the lineage hierarchy contribute to fate diversification of hypothalamic neurons in a stepwise fashion, suggesting a cascade diversification model that deconstructs the origin of neuronal diversity.
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http://dx.doi.org/10.1016/j.stem.2021.03.020DOI Listing
April 2021

The Functional and Antiviral Activity of Interferon Alpha-Inducible IFI6 Against Hepatitis B Virus Replication and Gene Expression.

Front Immunol 2021 1;12:634937. Epub 2021 Apr 1.

State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, China.

Hepatitis B virus is an enveloped DNA virus, that infects more than three hundred and sixty million people worldwide and leads to severe chronic liver diseases. Interferon-alpha inducible protein 6 (IFI6) is an IFN-stimulated gene (ISG) whose expression is highly regulated by the stimulation of type I IFN-alpha that restricts various kinds of virus infections by targeting different stages of the viral life cycle. This study aims to investigate the antiviral activity of IFI6 against HBV replication and gene expression. The IFI6 was highly induced by the stimulation of IFN-α in hepatoma cells. The overexpression of IFI6 inhibited while knockdown of IFI6 elevated replication and gene expression of HBV in HepG2 cells. Further study determined that IFI6 inhibited HBV replication by reducing EnhII/Cp of the HBV without affecting liver enriched transcription factors that have significant importance in regulating HBV enhancer activity. Furthermore, deletion mutation of EnhII/Cp and CHIP analysis revealed 100 bps (1715-1815 nt) putative sites involved in IFI6 mediated inhibition of HBV. Detailed analysis with EMSA demonstrated that 1715-1770 nt of EnhII/Cp was specifically involved in binding with IFI6 and restricted EnhII/Cp promoter activity. Moreover, IFI6 was localized mainly inside the nucleus to involve in the anti-HBV activity of IFI6. analysis based on the hydrodynamic injection of IFI6 expression plasmid along with HBV revealed significant inhibition of HBV DNA replication and gene expression. Overall, our results suggested a novel mechanism of IFI6 mediated HBV regulation that could develop potential therapeutics for efficient HBV infection treatment.
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http://dx.doi.org/10.3389/fimmu.2021.634937DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8047077PMC
April 2021

Antiviral Activity of Interferon Alpha-Inducible Protein 27 Against Hepatitis B Virus Gene Expression and Replication.

Front Microbiol 2021 31;12:656353. Epub 2021 Mar 31.

State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, China.

Despite the availability of effective vaccines, hepatitis B virus (HBV) is still a major health issue, and approximately 350 million people have been chronically infected with HBV throughout the world. Interferons (IFNs) are the key molecules in the innate immune response that restrict several kinds of viral infections the induction of hundreds of IFN-stimulated genes (ISGs). The objective of this study was to confirm if interferon alpha-inducible protein 27 (IFI27) as an ISG could inhibit HBV gene expression and DNA replication both in cell culture and in a mouse model. In human hepatoma cells, IFI27 was highly induced by the stimulation of IFN-alpha (IFN-α), and it potentiated the anti-HBV activity. The overexpression of IFI27 inhibited, while its silencing enhanced the HBV replication in HepG2 cell. However, the knocking out of IFI27 in HepG2 cells robustly increases the formation of viral DNA, RNA, and proteins. Detailed mechanistic analysis of the HBV genome showed that a sequence [nucleotide (nt) 1715-1815] of the EnhII/Cp promoter was solely responsible for viral inhibition. Similarly, the hydrodynamic injection of IFI27 expression constructs along with the HBV genome into mice resulted in a significant reduction in viral gene expression and DNA replication. In summary, our studies suggested that IFI27 contributed a vital role in HBV gene expression and replication and IFI27 may be a potential antiviral agent for the treatment of HBV.
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http://dx.doi.org/10.3389/fmicb.2021.656353DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044325PMC
March 2021

A scoping review on the influencing factors and development process of professional identity among nursing students and nurses.

J Prof Nurs 2021 Mar-Apr;37(2):391-398. Epub 2020 May 6.

Kiang Wu Hospital, 85-87 Estr. de Coelho do Amaral, Macau, China.

Background: Professional identity (PI) is culturally shaped. It is associated with a sufficient and stable workforce of professionals. China has a relatively low ratio of nursing professionals to its population.

Aim: This scoping review aims to obtain comprehensive knowledge of the influencing factors and PI development process among nursing students and nurses in China.

Methods: A scoping review was conducted. The most common Chinese databases, China National Knowledge Infrastructure and Wanfang Data were searched for publications in Chinese. The databases of EBSCOhost and ProQuest Dissertation & Thesis Global (Full Text) were searched for publications in English. After screening the title and abstract of the articles and further assessing the full text of the articles identified after the initial screening, 53 articles were included for analysis.

Results: The influencing factors to PI development in nursing were grouped into four dimensions: personal, family, institutional, and social factors. The social factors tended to negatively affect professional identity whereas the factors of the three other dimensions exerted influence in different directions. A framework was established based on PI levels in different career stages of nurses to depict the continuum and dynamic nature of the development process.

Conclusions: The PI development in nursing is a dynamic process shaped by multidimensional factors. Changes in policy should be made to reverse the nursing profession stereotype of being an assistant role to medicine.
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http://dx.doi.org/10.1016/j.profnurs.2020.04.018DOI Listing
June 2021