Publications by authors named "Mengyu Zhang"

83 Publications

A new classification of congenital abnormalities of UPVS: sonographic appearances, screening strategy and clinical significance.

Insights Imaging 2021 Sep 6;12(1):125. Epub 2021 Sep 6.

Department of Ultrasound, Affiliated Shenzhen Maternity and Child Healthcare Hospital, Southern Medical University, Hongli Road No. 2004, Futian, Shenzhen, 518028, Guangdong, China.

The umbilical-portal venous system (UPVS) plays an important role in embryonic development, as well as a significant blood circulation system to ensure the normal blood supply of fetal heart and brain and other vital organs. Congenital anomalies of UPVS contain many subtypes with a broad spectrum of manifestations and prognoses. Furthermore, because of fetal small lumen of UPVS, the sonographic evaluation remains difficult in utero. Appreciation of normal embryology and anatomy of UPVS is essential to an understanding of sonographic characteristics of anomalies of UPVS and fetal sequential changes. Through reviewing previous references and our experience with congenital abnormalities of UPVS, a new comprehensive classification is proposed. The new classification identifies three types of congenital abnormalities of UPVS based on morphological abnormalities and shunts. The embryology and etiology, sonographic, clinical and prognostic characteristics of each subtype of the new classification are described in detail. Knowledge of congenital abnormalities of UPVS can give sonographers a clue and aid prenatal sonographic diagnosis. The purpose of this article is to help the sonographers to understand the new classification of congenital abnormalities of UPVS, master the sonographic characteristics of each subtype and prenatal ultrasonographic screening strategy, and guide subsequent appropriate counseling and management.
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http://dx.doi.org/10.1186/s13244-021-01068-5DOI Listing
September 2021

Oleanolic acid inhibits the migration and invasion of hepatocellular carcinoma cells by promoting microRNA-122 expression.

Pharmazie 2021 Sep;76(9):422-427

Department of Dermatology, Chongqing Traditional Chinese Medicine Hospital, Chongqing, China;, Email:

MicroRNAs are emerging as important endogenous regulators of gene function and they are playing an important role in the occurrence and development of cancer. They are also regarded as robust biomarkers of cancer diagnosis and prognosis. Hepatocellular carcinoma (HCC) is a common and complex human malignancy with high mortality and morbidity in the world. MicroRNA-122 (miR-122) is a liver-specific microRNA and is closely associated with HCC metastasis, which makes miR-122 a promising target for drug design and development. In this study, we performed a cell-based screening method for discovering miR-122 activators and found that oleanolic acid (OA), a natural pentacyclic triterpene, specifically increased miR-122 expression in a concentration-dependent manner. Two HCC cell lines (HepG2 and Sk-hep-1 cells) were used to evaluate the effect of OA on cell migration and invasion abilities. The results indicated that OA attenuated the migration and invasion abilities of HCC cells by upregulating miR-122 expression. In addition, OA increased the expression of E-cadherin and decreased the expression of β-catenin, N-cadherin and vimentin. After knocking down miR-122 with miR-122 inhibitor, we found that the effect of OA on these epithelial-to-mesenchymal transition (EMT) related molecules was significantly weakened, indicating OA exhibited anti-EMT effect by increasing the expression of miR-122. These finding may help to better understand the molecular mechanism of OA's anti-metastasis activity.
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http://dx.doi.org/10.1691/ph.2021.1366DOI Listing
September 2021

The Number of Interstitial Cells of Cajal Differs Among Different Subtypes of Achalasia and is Related to Patients' Prognosis.

Clin Transl Gastroenterol 2021 Aug 24;12(8):e00388. Epub 2021 Aug 24.

Department of Gastroenterology, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.

Introduction: Achalasia is a primary esophageal motility disorder with heterogeneous manometric subtypes and prognosis, characterized by degeneration of the esophageal myenteric plexus, and reduction in interstitial cells of Cajal (ICCs). This study aimed to explore the histopathologic characteristics of lower esophageal sphincter (LES) muscle from patients with achalasia with different subtypes and different prognosis.

Methods: We examined specimens of LES muscle from 122 patients with achalasia who underwent peroral endoscopic myotomy and from 10 control patients who underwent esophagectomy for esophageal cancer. Hematoxylin-eosin staining was performed to assess inflammation infiltration, fibrosis, and atrophy. Specific immunohistochemical staining was performed to identify ICCs and neuronal nitric oxide synthase (nNOS).

Results: The number of ICCs in patients with type I achalasia was significantly lower than that in patients with type II achalasia, followed by that in control patients (type I vs type II vs control group= 0.4 vs 1.2 vs 9.5; P < 0.001). The number of nNOS-positive cells was significantly lower in patients with achalasia than that in control patients (type I vs type II vs control group = 0.0 vs 0.0 vs 8.0; P < 0.001). Nonrecurrent group had significantly more ICCs than recurrent group (type I: nonrecurrent vs recurrent = 1.0 vs 0.1; P = 0.010; type II: nonrecurrent vs recurrent = 2.0 vs 0.4; P = 0.004).

Discussion: ICCs and nNOS-positive cells reduced significantly in LES muscle of patients with achalasia. The number of ICCs differed among different achalasia subtypes and was related to patients' clinical prognosis.
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http://dx.doi.org/10.14309/ctg.0000000000000388DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8386899PMC
August 2021

Long-term predictions of current confirmed and dead cases of COVID-19 in China by the non-autonomous delayed epidemic models.

Cogn Neurodyn 2021 Jul 26:1-10. Epub 2021 Jul 26.

School of Mathematics and Statistics, Zhengzhou University, Zhengzhou, 450001 Henan China.

In this paper, we make long-term predictions based on numbers of current confirmed cases, accumulative dead cases of COVID-19 in different regions in China by modeling approach. Firstly, we use the SIRD epidemic model (S-Susceptible, I-Infected, R-Recovered, D-Dead) which is a non-autonomous dynamic system with incubation time delay to study the evolution of the COVID-19 in Wuhan City, Hubei Province and China Mainland. According to the data in the early stage issued by the National Health Commission of China, we can accurately estimate the parameters of the model, and then accurately predict the evolution of the COVID-19 there. From the analysis of the issued data, we find that the cure rates in Wuhan City, Hubei Province and China Mainland are the approximately linear increasing functions of time and their death rates are the piecewisely decreasing functions. These can be estimated by finite difference method. Secondly, we use the delayed SIRD epidemic model to study the evolution of the COVID-19 in the Hubei Province outside Wuhan City. We find that its cure rate is an approximately linear increasing function and its death rate is nearly a constant. Thirdly, we use the delayed SIR epidemic model (S-Susceptible, I-Infected, R-Removed) to predict those of Beijing, Shanghai, Zhejiang and Anhui Provinces. We find that their cure rates are the approximately linear increasing functions and their death rates are the small constants. The results indicate that it is possible to make accurate long-term predictions for numbers of current confirmed, accumulative dead cases of COVID-19 by modeling. In this paper the results indicate we can accurately obtain and predict the turning points, the end time and the maximum numbers of the current infected and dead cases of the COVID-19 in China. In spite of our simple method and small data, it is rather effective in the long-term prediction of the COVID-19.
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http://dx.doi.org/10.1007/s11571-021-09701-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8312358PMC
July 2021

The characteristic of N-nitrosodimethylamine precursor release from algal organic matter and degradation performance of UV/HO/O technology.

Sci Total Environ 2021 Nov 28;795:148739. Epub 2021 Jun 28.

Shandong Province Water Supply and Drainage Monitoring Center, 250101 Jinan, China.

Seasonal cyanobacterial blooms in eutrophic water releases algal organic matter (AOM), which contains large amount of dissolved organic nitrogen (DON) and is difficult to be removed effectively by conventional treatment processes (e.g., coagulation and sand filtration) because of its high hydrophilicity. Moreover, N-nitrosodimethylamine (NDMA) can be generated by the reaction of AOM with disinfectants in the subsequent disinfection process. In this study, the formation of NDMA from different AOM components was explored and the control of algal-derived NDMA precursors by UV/HO/O was evaluated. The results showed that the hydrophilic and polar components of AOM with the low molecular weight had higher NDMA yields. UV-based advanced oxidation process (AOPs) is effective in degrading NDMA precursors, while the removal rate can be affected greatly by UV doses. The removal rate of NDMA precursors by UV/HO/O is higher than by UV/HO or UV/O which can reach 95% at the UV dose of 400 mJ/cm. An alkaline environment reduces the oxidation efficiency of UV/HO/O technology, while an acidic environment is conducive to its function. Inorganic anions such as HCO, SO, Cl and NO are potential to compete with target algal-derived NDMA precursors for the oxidants reaction and inhibit the degradation/removal of these precursors. The degradation of algal-derived NDMA precursors by UV/HO/O is mainly accomplished by the oxidation of DON with secondary amide groups, and the main degradation mechanism by UV/HO/O was through the initial decomposition of macromolecular organic compounds such as biopolymers and humic substances and the further degradation of resulting small molecular components.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148739DOI Listing
November 2021

Development and Validation of an LC-MS/MS Method to Simultaneously Measure Tacrolimus and Everolimus Concentrations in Kidney Allograft Biopsies after Kidney Transplantation.

Ther Drug Monit 2021 Jun 28. Epub 2021 Jun 28.

Department of Clinical Pharmacology and Biopharmaceutics, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan Department of Pharmacy, Kyushu University Hospital, Fukuoka, Japan Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan Department of Medicine and Clinical Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.

Background: Therapeutic drug monitoring (TDM) is necessary for immunosuppressive therapy with tacrolimus and everolimus after kidney transplantation. Several studies have suggested that the concentrations of immunosuppressive agents in allografts may better reflect clinical outcomes than whole blood concentrations. This study aimed to develop a method for the simultaneous quantification of tacrolimus and everolimus concentrations in clinical biopsy samples and investigate their correlation with histopathological findings in kidney transplant recipients.

Methods: Fourteen biopsy samples were obtained from kidney transplant recipients at 3 months after transplantation. Kidney allograft concentrations (Ctissue) of tacrolimus and everolimus were measured by liquid chromatography tandem mass spectrometry (LC-MS/MS), and the corresponding whole blood trough concentrations (C0) were obtained from clinical records.

Results: The developed method was validated over a concentration range of 0.02[FIGURE DASH]2.0 ng/mL for tacrolimus and 0.04[FIGURE DASH]4.0 ng/mL for everolimus in kidney tissue homogenate. The Ctissue of tacrolimus and everolimus in kidney biopsies ranged from 21.0 to 86.7 pg/mg tissue and 33.5 to 105.0 pg/mg tissue, respectively. Dose-adjusted Ctissue of tacrolimus and everolimus was significantly correlated with the dose-adjusted C0 (P < 0.0001 and P = 0.0479, respectively). No significant association was observed between the Ctissue of tacrolimus and everolimus and the histopathologic outcomes at 3 months after transplantation.

Conclusions: This method could support further investigation of the clinical relevance of tacrolimus and everolimus allograft concentrations after kidney transplantation.
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http://dx.doi.org/10.1097/FTD.0000000000000912DOI Listing
June 2021

Upright Integrated Relaxation Pressure Predicts Symptom Outcome for Esophagogastric Junction Outflow Obstruction.

J Neurogastroenterol Motil 2021 Jul;27(3):363-369

Department of Gastroenterology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.

Background/aims: Esophagogastric junction outflow obstruction (EGJOO) is characterized by elevated integrated relaxation pressure (IRP) and preserved esophageal peristalsis. The clinical significance of EGJOO is uncertain. This study aim to describe the clinical characteristics of these patients and to find out potential parameters to predict patients' symptom outcome.

Methods: Consecutive patients who received high-resolution manometry examination in our hospital in 2013-2019 and met the diagnostic criteria of EGJOO were retrospectively included. Motility and reflux parameters as well as endoscopy and barium esophagogram results were studied and compared. Patients were also followed up to record their treatment methods and symptom outcomes.

Results: A total of 138 EGJOO (accounting for 5.2% of total patients taking high-resolution manometry examination in our hospital) patients were included. Only 2.9% of these patients had persistent dysphagia. A total of 81.8% of EGJOO patients had symptom resolution during follow-up. Patients with persistent dysphagia had significantly higher upright IRP (16.6 [10.3, 19.8] vs 7.8 [3.2, 11.5]; = 0.026) than those without. Upright IRP can effectively distinguished patients with persistent dysphagia (area under curve: 0.826; = 0.026) using optimal cut-off value of 9.05 mmHg.

Conclusion: EGJOO patients with persistent dysphagia and higher upright IRP (median > 9.05 mmHg) needs further evaluation and aggressive management.
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http://dx.doi.org/10.5056/jnm20106DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8266504PMC
July 2021

Esophageal mucosal sensory nerves and potential mechanoreceptors in patients with ineffective esophageal motility.

Neurogastroenterol Motil 2021 Jun 21:e14205. Epub 2021 Jun 21.

Wingate Institute of Neurogastroenterology, Blizard Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, UK.

Background: Ineffective esophageal motility (IEM) is the most common motility disorder. However, little is known about its pathophysiology. Vagal afferent nerves convey esophageal intraluminal bolus information to solitary nucleus, which is likely to be involved with esophageal primary and secondary peristalsis (SP). We hypothesized that altered mucosal sensory afferents underlie the pathogenesis of IEM.

Methods: We prospectively collected esophageal biopsies from 38 patients with proton pump inhibitor-refractory reflux symptoms from January to December 2019. All patients underwent high-resolution manometry for the evaluation of primary and secondary peristalsis, and off-PPI 24-h impedance-pH studies. Biopsies were analyzed using immunohistochemistry for identification of calcitonin gene-related peptide-immunoreactive (CGRP-IR) nerves and qPCR for mRNA expression of potential mechanoreceptors.

Key Results: Overall 32 patients were finally analyzed which consisted of 11 patients with normal motility and 21 patients with IEM. The position of mucosal CGRP-IR nerves from the esophageal lumen did not differ between the two groups (the proximal esophagus (p = 0.52), the mid-esophagus (p = 0.92), the distal esophagus (p = 0.29)) with the similar reflux profile. No difference was seen in the position of CGRP-IR nerves between patients with successful triggering of SP and those unable to trigger SP. There was also no difference in mRNA expression of each potential mechanoreceptors (TRPA1, TRPV1, TRPV4, ASIC1, ASIC3) between the two groups.

Conclusions And Inferences: Our study showed that mucosal sensory afferents nerve position and mRNA expression of potential mechanoreceptors did not correlate to weak esophageal contraction.
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http://dx.doi.org/10.1111/nmo.14205DOI Listing
June 2021

Protective effects of sulfated polysaccharides from Lentinula edodes on the lung and liver of MODS mice.

Food Funct 2021 Jul 31;12(14):6389-6402. Epub 2021 May 31.

College of Life Science, Shandong Agricultural University, Taian 271018, PR China. :

In this work, the effects of sulfated polysaccharides from Lentinula edodes (SPLE) on zymosan (ZYM)-induced multiple organ dysfunction syndrome (MODS) mice were investigated. Using the MODS mice model, biochemical works have already shown that in mice treated with SPLE, the lung parameters of GGT, C3 and hs-CRP were down-regulated and the hepatic parameters of TC, TG, ALT and AST, HDLC, LDL-C and VLDL-C were improved, the serum levels of CK, Cr and Amy were decreased, and the levels of inflammatory factors such as TNF-α, IL-1β, IL-6 and IL-10 were also reduced, the activity of antioxidant enzymes SOD and CAT enhanced, and the content of MDA was reduced. In addition, histopathology of the lung and liver confirmed the beneficial effects of SPLE on MODS mice, indicating that SPLE played a role in protecting the organ function of MODS mice. In addition, SPLE was characterized as a sulfated β-glucan linked by β-type glycosidic bonds. These conclusions indicated that SPLE had effective antioxidant and anti-inflammatory activities, and could be used as a functional food and medicine to prevent MODS.
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http://dx.doi.org/10.1039/d1fo00399bDOI Listing
July 2021

Effect of dietary Xiao-Chaihu-Decoction on growth performance, immune response, detoxification and intestinal microbiota of pacific white shrimp (Litopenaeus vannamei).

Fish Shellfish Immunol 2021 Jul 7;114:320-329. Epub 2021 May 7.

The Key Laboratory of Mariculture, Ocean University of China, Ministry of Education, Qingdao, Shandong, 266003, China.

Xiao-Chaihu-Decoction (XCHD), a classical traditional Chinese medicine with diverse biological activities, is widely applied to prevent and treat many human diseases. Effects of dietary XCHD on growth performance, immune response, detoxification system, intestinal microbiota and resistance against aflatoxin B1(AFB) of Litopenaeus vannamei was studied. Four isonitrogenous and isolipidic diets were formulated to contain 0, 1, 2, and 5 g/kg (control, XCHD1, XCHD2 and XCHD3) of XCHD, respectively. Seven hundred and eighty shrimp (1.16 ± 0.09 g) were assigned randomly to 12 tanks (400 L, three tanks each group, 65 shrimp in each tank) for 6 weeks. After sampling, 25 shrimp from each tank were selected for a 2-week AFB (2500 μg/kg) challenge experiment. The results indicated that the final weight, weight gain and specific growth rate in XCHD2 and XCHD3 groups were significantly increased compared to control. The protease, amylase, superoxide dismutase (SOD), glutathione s-transferase (GST), sulfotransferase (SULT) activities, total antioxidant capacity (T-AOC) and glutathione (GSH) contents in hepatopancreas were significantly increased in XCHD3 groups and the expressions of immune-related genes (Toll, Dorsal and Cru) in hepatopancreas were significantly up-regulated in XCHD2 and XCHD3 groups. High-throughput sequencing analysis revealed that the abundance of Proteobacteria decreased and the abundances of Bacteroidetes increased in XCHD2 and XCHD3 groups. Additionally, AFB challenge experiments showed that AFB caused histological damage to the hepatopancreas and significantly increased the levels of malondialdehyde (MDA) and protein carbonylation (PC) in hepatopancreas as well as the activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Nevertheless, XCHD could effectively alleviated the growth toxicity, immunosuppression and macromolecular damage caused by AFB to shrimp by inhibiting the Phase I enzyme and enhancing Phase II enzyme and antioxidant system.
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http://dx.doi.org/10.1016/j.fsi.2021.05.005DOI Listing
July 2021

Preparation of an amphiphilic Janus SiO/fluorinated polyacrylate latex film and its application as a hydrophobic fabric agent.

J Colloid Interface Sci 2021 Oct 16;599:88-99. Epub 2021 Apr 16.

College of Bioresources Chemical and Materials Engineering, Shaanxi University of Science & Technology, Xi'an 710021, Shaanxi, China; National Demonstration Center for Experimental Light Chemistry Engineering Education Shaanxi University of Science & Technology, Xi'an 710021, Shaanxi, China; Xi'an Key Laboratory of Green Chemicals and Functional Materials, Shaanxi University of Science and Technology, Xi'an 710021, China.

Amphiphilic Janus particles are characterized by their anisotropic morphology and unique physical and chemical properties. In the present research, amphiphilic Janus particles were used as stabilizing agents to prepare a fluorine-containing polyacrylate composite emulsion. The influences of the structure and dosage of amphiphilic Janus SiO particles and the amount of fluorine-containing monomer hexafluorobutyl methacrylate on the stability of the composite emulsion were investigated. It was noticed that when the hydrophilic and hydrophobic groups of Janus SiO particles were polyacrylamide and polymethyl methacrylate, respectively, the stabilization of the polyacrylate emulsion with Janus SiO particles was achieved. When 0.3 wt% of polyacrylamide/polymethyl methacrylate amphiphilic Janus SiO particles and 8 wt% of hexafluorobutyl methacrylate were used, a stable composite emulsion was obtained. The conversion rate reached 98.7% with an average particle size of 500 nm. The composite emulsion was applied for fabric finishing. The water contact angle of the fabric increased from 21.4° to 140.2°, demonstrating its greatly improved hydrophobicity. Therefore, it could be inferred that the synergistic effect of amphiphilic Janus SiO nanoparticles and hexafluorobutyl methacrylate improved the water resistance of the latex film.
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http://dx.doi.org/10.1016/j.jcis.2021.04.061DOI Listing
October 2021

Protective effect of selenomethionine on T-2 toxin-induced liver injury in New Zealand rabbits.

BMC Vet Res 2021 Apr 9;17(1):153. Epub 2021 Apr 9.

College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, 471000, Henan, China.

Background: T-2 toxin is a mycotoxin produced by Fusarium species that is highly toxic to animals. Recent studies have indicated that Selenomethionine (SeMet) have protective effect against mycotoxins-induced toxicity. The aim of the present study was to investigate the protective effect of SeMet on T-2-toxin-induced liver injury in rabbit and explore its molecular mechanism. Fifty rabbits (30 d, 0.5 ± 0.1 kg) were randomly divided into 5 groups: control group, T-2 toxin group, low, medium and high dose SeMet treatment group. The SeMet-treated group was orally pretreated with SeMet (containing selenium 0.2 mg/kg, 0.4 mg/kg and 0.6 mg/kg) for 21 days. On the 17th day, T-2 toxin group and SeMet-treated group were orally administered with T-2 toxin (0.4 mg/kg body weight) for 5 consecutive days.

Results: The results showed that low-dose SeMet significantly improved T-2 toxin-induced liver injury. We found that low-dose SeMet can reduce the level of oxidative stress and the number of hepatocyte apoptosis. Moreover, the levels of Bax, caspase-3 and caspase-9 were significantly reduced and the levels of Bcl-2 were increased.

Conclusions: Therefore, we confirmed that low-dose SeMet may protect rabbit hepatocytes from T-2 toxin by inhibiting the mitochondrial-caspase apoptosis pathway.
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http://dx.doi.org/10.1186/s12917-021-02866-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8033731PMC
April 2021

Speech emotion recognition based on transfer learning from the FaceNet framework.

J Acoust Soc Am 2021 02;149(2):1338

College of Computing and Software Engineering, Kennesaw State University, Marietta, Georgia 30060, USA.

Speech plays an important role in human-computer emotional interaction. FaceNet used in face recognition achieves great success due to its excellent feature extraction. In this study, we adopt the FaceNet model and improve it for speech emotion recognition. To apply this model for our work, speech signals are divided into segments at a given time interval, and the signal segments are transformed into a discrete waveform diagram and spectrogram. Subsequently, the waveform and spectrogram are separately fed into FaceNet for end-to-end training. Our empirical study shows that the pretraining is effective on the spectrogram for FaceNet. Hence, we pretrain the network on the CASIA dataset and then fine-tune it on the IEMOCAP dataset with waveforms. It will derive the maximum transfer learning knowledge from the CASIA dataset due to its high accuracy. This high accuracy may be due to its clean signals. Our preliminary experimental results show an accuracy of 68.96% and 90% on the emotion benchmark datasets IEMOCAP and CASIA, respectively. The cross-training is then conducted on the dataset, and comprehensive experiments are performed. Experimental results indicate that the proposed approach outperforms state-of-the-art methods on the IEMOCAP dataset among single modal approaches.
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http://dx.doi.org/10.1121/10.0003530DOI Listing
February 2021

Selenium mediated host plant-mite conflict: defense and adaptation.

Pest Manag Sci 2021 Jun 8;77(6):2981-2989. Epub 2021 Mar 8.

Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.

Background: Selenium has shown effectiveness in protecting plants from herbivores. However, some insects have evolved adaptability to selenium.

Results: Selenium accumulation in host plants protected them against spider mite feeding. Selenium showed toxic effects on spider mites by reducing growth and interfering with reproduction. After 40 generations on selenium-rich plants, a Tetranychus cinnabarinus strain (Tc-Se) developed adaptability to selenium, with an increased rate of population growth and enhanced ability for selenium metabolism. The high expression of two genes (GSTd07 and SPS1) in the selenium metabolism pathway might be involved in selenium metabolism in spider mites. After GSTd07 and SPS1 were silenced, the selenium adaptability decreased. Recombinant GSTd07 protein promoted the reaction between sodium selenite and glutathione (GSH) and increased the production of sodium selenite metabolites. The results indicated that GSTd07 was involved in the first step of selenium metabolism.

Conclusion: Plants can resist spider mite feeding by accumulating selenium. Spider mites subjected to long-term selenium exposure can adapt to selenium by increasing the expression of key genes involved in selenium metabolism. These results elucidate the mechanism of the interaction between mites and host plants mediated by selenium. This study of the interaction between selenium-mediated host plants and spider mites may lead to the development of new and less toxic methods for the prevention and control of spider mites. © 2021 Society of Chemical Industry.
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http://dx.doi.org/10.1002/ps.6337DOI Listing
June 2021

AURKA and FAM83A are prognostic biomarkers and correlated with Tumor-infiltrating Lymphocytes in smoking related Lung Adenocarcinoma.

J Cancer 2021 18;12(6):1742-1754. Epub 2021 Jan 18.

Department of Pulmonary and Critical Care Medicine, Qilu Hospital of Shandong University, Jinan 250012, China.

Lung adenocarcinoma (LUAD) has become the main histologic type, which account for nearly 40% of lung cancer. The present study aimed to investigate the gene expression signature in smoking related LUAD. A total of 45 smoking related DEGs in LUAD were identified and functional enrichment analysis was also performed. Then Cox's regression model and Kaplan-Meier analysis were used to screen potential prognostic genes. Finally, AURKA and FAM83A were left for further immune-related mechanism exploration. Kaplan-Meier analysis indicated survival rates are related to different immune cell (B cell and Dendritic cell) infiltration levels. Mechanistically, we further explore the correlation between AURKA and FAM83A gene expression levels and tumor-infiltrating lymphocytes (TILs) level as well as their response to immunomodulators. The results suggested that AURKA and FAM83A are highly expressed in smoking related LUAD, and negatively correlated to B cell and Dendritic cell infiltration levels. At the same time, B cell and Dendritic cell infiltration levels also related to the prognosis of LUAD. We further revealed AURKA and FAM83A could be novel targets to improve the prognosis of LUAD through regulated the response to immunomodulators.
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http://dx.doi.org/10.7150/jca.51321DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7890332PMC
January 2021

A modified membrane filtration-ultraviolet photocatalytic system for the removal of trace sulfadiazine in drinking water (No. CHEM77354R1).

Chemosphere 2021 Jun 9;272:129867. Epub 2021 Feb 9.

School of Water Conservancy & Civil Engineering, Shandong Agricultural University, Taian, 271000, PR China. Electronic address:

In this paper, the membrane filtration-photocatalytic coupling process was used to explore the mechanism and removal effect of trace concentrations of sulfadiazine (SD) in drinking water. First, 8 kinds of ultrafiltration membranes were successfully prepared, and their performance was verified by scanning electron microscopy and measurement of the contact angle, membrane pure water flux, porosity and average pore size. The results showed that the best-performing membranes were the PVDF-PP-TiO-DA (dopamine) (PPTD)- and PVDF-PP-TiO-FeCl (PPTFe)-modified ultrafiltration membranes, in which TiO was modified with DA and FeCl, forming the cooperation of TiO/DA and TiO/Fe, with removal rates of 91.4% and 92.6% and quasi-first-order rates of 0.0216 min-1 and 0.0214 min-1. At the same time, the effects of the two types of membrane, UV light and water quality characteristics on the removal performance of the membrane filtration-photocatalytic system were discussed. Among them, the PPTD membrane was more suitable than the other membranes for the degradation of weakly alkaline water containing SD (pH = 7.5), except when NO was present, and the water quality characteristics had a significant inhibitory effect on the removal effect. The PPTFe membrane was more suitable for the degradation of acidic water containing SD (pH = 3). Additionally, the water quality characteristics had an obvious inhibitory effect on the removal effect, and the accuracy of the water distribution experimental results was verified by using an actual body of water. In the end, the reaction mechanism of the filtration-photocatalytic system was proposed, and it was found that OH played an indispensable role in the removal of SD.
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http://dx.doi.org/10.1016/j.chemosphere.2021.129867DOI Listing
June 2021

Nitrogen removal performance, quantitative detection and potential application of a novel aerobic denitrifying strain, Pseudomonas sp. GZWN4 isolated from aquaculture water.

Bioprocess Biosyst Eng 2021 Jun 18;44(6):1237-1251. Epub 2021 Feb 18.

The Key Laboratory of Mariculture, Ministry of Education, Fisheries College, Ocean University of China, Yushan Road 5, Qingdao, 266003, Shandong, China.

A novel Pseudomonas sp. GZWN4 with the aerobic nitrogen removal ability was isolated from aquaculture water, whose removal efficiency of NO-N, NO-N and NH-N was 99.72%, 82.54% and 98.62%, respectively. The key genes involved in nitrogen removal, nxr, napA, narI, nirS, norB and nosZ, were successfully amplified and by combination with the results of nitrogen balance analysis, it was inferred that the denitrification pathway of strain GZWN4 was NO-N → NO-N → NO → NO → N. The strain GZWN4 had excellent nitrite removal performance at pH 7.0-8.5, temperature 25-30 ℃, C/N ratio 5-20, salinity 8-32‰ and dissolved oxygen concentration 2.52-5.73 mg L. The receivable linear correlation (R = 0.9809) was obtained with the range of quantification between l0 and 10 CFU mL of the strain by enzyme-linked immunosorbent assay. Strain GZWN4 could maintain high abundance in the actual water and wastewater of mariculture and the removal efficiency of TN were 52.57% and 63.64%, respectively. The safety evaluation experiment showed that the strain GZWN4 had no hemolysis and high biosecurity toward shrimp Litopenaeus vannamei. The excellent nitrogen removal ability and adaptability to aquaculture environment made strain GZWN4 a promising candidate for treatment of water and wastewater in aquaculture.
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http://dx.doi.org/10.1007/s00449-021-02523-9DOI Listing
June 2021

The COMPASS-like complex modulates fungal development and pathogenesis by regulating H3K4me3-mediated targeted gene expression in Magnaporthe oryzae.

Mol Plant Pathol 2021 04 8;22(4):422-439. Epub 2021 Feb 8.

Beijing Key Laboratory of New Technology in Agricultural Application, National Demonstration Center for Experimental Plant Production Education, Beijing University of Agriculture, Beijing, China.

Histone-3-lysine-4 (H3K4) methylation is catalysed by the multiprotein complex known as the Set1/COMPASS or MLL/COMPASS-like complex, an element that is highly evolutionarily conserved from yeast to humans. However, the components and mechanisms by which the COMPASS-like complex targets the H3K4 methylation of plant-pathogenic genes in fungi remain elusive. Here we present a comprehensive analysis combining biochemical, molecular, and genome-wide approaches to characterize the roles of the COMPASS-like family in the rice blast fungus Magnaporthe oryzae, a model plant pathogen. We purified and identified six conserved subunits of COMPASS from M. oryzae: MoBre2 (Cps60/ASH2L), MoSpp1 (Cps40/Cfp1), MoSwd2 (Cps35), MoSdc1 (Cps25/DPY30), MoSet1 (MLL/ALL), and MoRbBP5 (Cps50), using an affinity tag on MoBre2. We determined the sequence repeat in dual-specificity kinase splA and ryanodine receptors domain of MoBre2 can interact directly with the DPY30 domain of MoSdc1 in vitro. Furthermore, we found that deletion of the genes encoding COMPASS subunits of MoBre2, MoSPP1, and MoSwd2 caused similar defects regarding invasive hyphal development and pathogenicity. Genome-wide profiling of H3K4me3 revealed that it has remarkable co-occupancy at the transcription start site regions of target genes. Significantly, these target genes are often involved in spore germination and pathogenesis. Decreased gene expression caused by the deletion of MoBre2, MoSwd2, or MoSpp1 was highly correlated with a decrease in H3K4me3. These results suggest that MoBre2, MoSpp1, and MoSwd2 function as a whole COMPASS complex, contributing to fungal development and pathogenesis by regulating H3K4me3-targeted genes in M. oryzae.
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http://dx.doi.org/10.1111/mpp.13035DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7938624PMC
April 2021

Effects of safflower yellow on cholesterol levels in serum and brain tissue of APP/PS1 mice.

Metab Brain Dis 2021 04 6;36(4):557-569. Epub 2021 Feb 6.

Key Laboratory of Xinjiang Phytomedicine Resource and Utilization, Ministry of Education, Department of Pharmacology, Shihezi University, Shihezi, Xinjiang, 832000, People's Republic of China.

Alzheimer's disease (AD) is an aggressive neurodegenerative disease associated with cognitive decline, memory, language, and visual-spatial coordination disorders that eventually lead to complete loss of basic function. Hypercholesterolemia plays an important role in the pathogenesis of AD and its related diseases. Safflower yellow (SY) is a natural chalcone compound isolated from safflower, which has the effect of antioxidation and weight loss. Previous studies have shown that SY has a significant improvement in learning and memory in various AD model animals. In the early stage of proteomic technology, we found that the cholesterol synthesis rate-limiting enzyme Mevalonate decarboxylase (MVD) was abnormally high in dementia rats, and the expression level of MVD decreased after SY treatment. We speculated that SY may improve the learning and memory ability of AD mice by affecting cholesterol metabolism. The purpose of this study was to evaluate the effect of SY on regulating cholesterol metabolism and improving dementia. The area of amyloid-β (Aβ) plaque in the brain of APP/PS1 mice and various blood biochemical and molecular biological indexes was detected. Through behavioral experiments, we found that APP/ PS1 mice had significant learning and memory impairment compared with wild type mice(P < 0.01). SY (30 mg/kg) treatment for 1 month can significantly improve the learning and memory ability of APP/PS1 mice (P < 0.01). Our results showed that SY decreased serum Total cholesterol (TC) and Triglyceride (TG) and increased the level of High-density lipoprotein (HDL). HE staining obscured that SY affect the changes of liver tissue in APP/PS1 mice (P < 0.05 and P < 0.01). We found that SY reduced the expression of MVD and Apolipoprotein E (APOE4) in the cortex (P < 0.05 and P < 0.01). In summary, SY can effectively control cholesterol in serum and brain and change the degeneration of liver tissue. SY improves Alzheimer's disease by lowering serum, cortex and cortical cholesterol.
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http://dx.doi.org/10.1007/s11011-021-00680-0DOI Listing
April 2021

Establishment of an experimental rat model of tacrolimus-induced kidney injury accompanied by interstitial fibrosis.

Toxicol Lett 2021 May 28;341:43-50. Epub 2021 Jan 28.

Department of Pharmacy, International University of Health and Welfare Narita Hospital, Japan; Department of Clinical Pharmacy, Faculty of Pharmaceutical Sciences, International University of Health and Welfare, Japan.

Nephrotoxicity is the major adverse reaction to tacrolimus; however, the underlying mechanisms remain to be fully elucidated. Although several tacrolimus-induced nephrotoxicity animal models have been reported, most renal injury rat models contain factors other than tacrolimus. Here, we report the development of a new nephrotoxicity with interstitial fibrosis rat model induced by tacrolimus administration. Thirty Wistar rats were randomly divided into four groups: sham-operated (Sham), vehicle-treated ischemia reperfusion (I/R) injury (IRI), tacrolimus treated (TAC) and tacrolimus treated I/R injury (TAC + IRI). Rats subjected to IR injury and treated with tacrolimus for 2 weeks showed higher serum creatinine (Scr), blood urea nitrogen (BUN), serum magnesium (Mg) and serum potassium (K), indicating decreased renal function. In addition, tacrolimus treatment combined with IR injury increased histological injury (tubular vacuolation, glomerulosclerosis and interstitial fibrosis), as well as α-smooth muscle actin (α-SMA), transforming growth factor-β (TGF-β), and kidney injury molecule-1 (KIM-1) expression in the renal cortex. In summary, we have developed a tacrolimus-induced kidney injury rat model with interstitial fibrosis within 2 weeks by creating conditions mimicking renal transplantation via tacrolimus administration following ischemia-reperfusion.
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http://dx.doi.org/10.1016/j.toxlet.2021.01.020DOI Listing
May 2021

MicroRNA-30/Cx43 axis contributes to podocyte injury by regulating ER stress in diabetic nephropathy.

Ann Transl Med 2020 Dec;8(24):1674

Department of Endocrinology, the Third Affiliated Hospital of Soochow University, Changzhou, China.

Background: The microRNA-30 family plays a critical role in the pathogenesis of podocyte injury. Cx43 plays an essential role in intercellular communication, which is essential for coordinated kidney function. This study was conducted to explore the function of microRNA-30s/Cx43 in podocyte injury in diabetic nephropathy (DN), both and .

Methods: SD rats were given streptozotocin (STZ) injections to induce DN. Podocytes were incubated in the medium in the presence or absence of high glucose (HG). The effects of the microRNA-30/Cx43 axis on DN and its underlying mechanisms were investigated by TUNEL assay, PAS, immunohistochemical staining, immunofluorescence staining, Western blot, RT-qPCR, RNA interference, and luciferase reporter assay. Podocytes were transfected with microRNA-30 family mimics, microRNA-30 family inhibitors, Cx43 siRNA, and negative controls to detect the effect of the microRNA-30/Cx43 axis. MicroRNA-30 family mimic AAVs, and microRNA-30 family inhibitor AAVs applied to regulate microRNA-30 family expression in the kidneys of the STZ-induced DN model rats to reveal the underlying mechanisms of the microRNA-30/Cx43 axis in DN.

Results: MicroRNA-30 family member expression was downregulated in HG-treated podocytes and the glomeruli of STZ-induced DN rats. Luciferase reporter assays confirmed Cx43 is a directed target of microRNA-30s. The overexpression of microRNA-30 family members attenuated the HG-induced podocyte injury and protected against podocyte apoptosis and endoplasmic reticulum stress (ERS) both and . Also, silencing Cx43 expression eased podocyte apoptosis, injury, and ERS induced by a HG+microRNA-30 family inhibitor. Double-immunofluorescence staining assays proved the co-localization of caspase12 and Cx43.

Conclusions: The overexpression of microRNA-30 family members prevents HG-induced podocyte injury and attenuates ERS by modulating Cx43 expression. The microRNA-30/Cx43/ERS axis might be a potential therapeutic target to treat DN.
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http://dx.doi.org/10.21037/atm-20-6989DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7812202PMC
December 2020

Heterotrophic nitrification-aerobic denitrification characteristics and antibiotic resistance of two bacterial consortia from Marinomonas and Halomonas with effective nitrogen removal in mariculture wastewater.

J Environ Manage 2021 Feb 9;279:111786. Epub 2020 Dec 9.

Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, Qingdao, Shandong, 266003, China.

Heterotrophic nitrification-aerobic denitrification (HNAD) characteristics and antibiotic resistance of two bacterial consortia, Marinomonas communis & Halomonas titanicae (MCH) and Marinomonas aquimarina & Halomonas titanicae (MAH), and their single isolates (MC, MA, and H) were determinated in this study. When cultured in sole and mixed N-source media (NH-N and/or NO-N of 10 mg/L), MCH and MAH exhibited greater efficiency and stability of inorganic-N removal than single isolates, and these strains preferred to remove NH-N by simultaneous HNAD in mixed N-source media. Meanwhile, 45%-70% of NH-N and/or NO-N was mainly converted to organic nitrogen (15%-25%) and gaseous nitrogen (30%-40%) by these strains, and more inorganic-N was transformed to intracellular-N by MCH and MAH via assimilation instead of gaseous-N production by denitrification. Both isolates and their consortia had the maximal NH-N or NO-N removal efficiency above 95% under the optimum conditions including temperature of 20-30 °C, C/N ratios of 15-20, and sucrose as carbon source. Interestingly, bacterial consortia performed greater nitrogen removal than single isolates under the low temperature of 10 °C or C/N ratios of 2-5. In real mariculture wastewater, MCH and MAH also showed higher NH-N removal efficiency (65%-68%) and more stable cell quantity (4.2-5.2 × 10 CFU/mL) than single strains, due to the interspecific coexistence detected by bacterial quantitation with indirect immunoassay. Additionally, these isolates and consortia had stronger resistances to polypeptides, tetracyclines, sulfonamides, furanes, and macrolides than other antibiotics. These findings will be conducive to the applications of HNAD bacteria of Marinomonas and Halomonas on reducing nitrogen pollution in mariculture or other saline environments.
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http://dx.doi.org/10.1016/j.jenvman.2020.111786DOI Listing
February 2021

Transcription response of Tetranychus cinnabarinus to plant-mediated short-term and long -term selenium treatment.

Chemosphere 2021 Jan 19;263:128007. Epub 2020 Aug 19.

Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China; Academy of Agricultural Sciences, Southwest University, Chongqing, China; State Cultivation Base of Crop Stress Biology for Southern Mountainous Land of Southwest University, Southwest University, Chongqing, China. Electronic address:

Selenium is a trace element necessary for living organisms. It exists mainly in the form of selenite in nature. In plants, selenium can enhance defenses against pests. In this study, transcriptome sequencing technology was used to analyze the response mechanism of Tetranychus cinnabarinus to plant-mediated selenium treatment. We tested four sodium selenite treatments (5, 20, 50, and 200 μM) that were the same for short (2 d) and long (30 d) treatment durations. The results showed that the number of differentially expressed genes (DEGs) in the short-term treatment was greater than in the long-term treatment. This indicated that the gene expression of spider mites gradually stabilized during the selenium treatment. Regardless of the long-term and short-term conditions, spider mites had the largest response to the 20 μM sodium selenite treatment. The functional annotation classification of DEGs showed no significant difference under different concentrations and treatment durations. A total of 25 genes were differentially expressed in all eight treatments, including four down-regulated cytochrome P450 genes and one up-regulated chitinase gene. We speculate that selenium may have the potential to enhance the activity of chemical acaricides. Transcriptome sequencing of sodium selenite treatment at different concentrations and different times revealed the response mechanism of spider mites under plant-mediated selenium treatment. At the same time, it also provides new clues for the development of methods for preventing and controlling spider mites with selenium.
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http://dx.doi.org/10.1016/j.chemosphere.2020.128007DOI Listing
January 2021

Simultaneous aerobic removal of phosphorus and nitrogen by a novel salt-tolerant phosphate-accumulating organism and the application potential in treatment of domestic sewage and aquaculture sewage.

Sci Total Environ 2021 Mar 11;758:143580. Epub 2020 Nov 11.

Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, 266003, Qingdao, China.

Phosphorus (P) and nitrogen (N) pollution are the worldwide challenging problem. In the present study, a new salt-tolerant phosphate-accumulating organism (PAO) was isolated and identified as Bacillus subtilis GHSP10. Strain GHSP10 did not produce hemolysin and showed high susceptibility to antibiotics. The favorable phosphorus removal C/N ratios, P/N ratios, temperature, salinities, pH values and shaking speeds of strain GHSP10 were 10-20, 0.1-0.2, 28 °C, 0-3%, 7.5-8.5 and 100-250 r/min. Besides, strain GHSP10 could conduct heterotrophic nitrification-aerobic denitrification and the maximal removal efficiencies of ammonium, nitrite and nitrate were 99.52%, 81.10% and 95.84% respectively. Moreover, the phosphorus removal process of strain GHSP10 was achieved under entirely aerobic conditions, and glycogen and poly-β-hydroxybutyrate could provide energy source for the phosphorus removal process of strain GHSP10. The amplification of ppk, hao, napA, narG, nirK genes as well as the expression of polyphosphate kinase helped to reveal the removal pathways of phosphorus and nitrogen, providing theoretical support for the phosphorus removal, nitrification and aerobic denitrification abilities of strain GHSP10. Furthermore, efficient removal of phosphorus and nitrogen from both domestic sewage and aquaculture sewage could be accomplished by strain GHSP10. This study may provide a hopeful candidate strain for simultaneous removal of phosphorus and nitrogen pollution from both freshwater sewage and saline sewage.
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http://dx.doi.org/10.1016/j.scitotenv.2020.143580DOI Listing
March 2021

Enhanced antitumor effects of follicle-stimulating hormone receptor-mediated hexokinase-2 depletion on ovarian cancer mediated by a shift in glucose metabolism.

J Nanobiotechnology 2020 Nov 7;18(1):161. Epub 2020 Nov 7.

Obstetrics and Gynecology Hospital, Fudan University, Shanghai, 200011, China.

Background: Most cancers favor glycolytic-based glucose metabolism. Hexokinase-2 (HK2), the first glycolytic rate-limiting enzyme, shows limited expression in normal adult tissues but is overexpressed in many tumor tissues, including ovarian cancer. HK2 has been shown to be correlated with the progression and chemoresistance of ovarian cancer and could be a therapeutic target. However, the systemic toxicity of HK2 inhibitors has limited their clinical use. Since follicle-stimulating hormone (FSH) receptor (FSHR) is overexpressed in ovarian cancer but not in nonovarian healthy tissues, we designed FSHR-mediated nanocarriers for HK2 shRNA delivery to increase tumor specificity and decrease toxicity.

Results: HK2 shRNA was encapsulated in a polyethylene glycol-polyethylenimine copolymer modified with the FSH β 33-53 or retro-inverso FSH β 33-53 peptide. The nanoparticle complex with FSH peptides modification effectively depleted HK2 expression and facilitated a shift towards oxidative glucose metabolism, with evidence of increased oxygen consumption rates, decreased extracellular acidification rates, and decreased extracellular lactate and glucose consumption in A2780 ovarian cancer cells and cisplatin-resistant A2780CP counterpart cells. Consequently, cell proliferation, invasion and migration were significantly inhibited, and tumor growth was suppressed even in cisplatin-resistant ovarian cancer. No obvious systemic toxicity was observed in mice. Moreover, the nanoparticle complex modified with retro-inverso FSH peptides exhibited the strongest antitumor effects and effectively improved cisplatin sensitivity by regulating cisplatin transport proteins and increasing apoptosis through the mitochondrial pathway.

Conclusions: These results established HK2 as an effective therapeutic target even for cisplatin-resistant ovarian cancer and suggested a promising targeted therapeutic approach.
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http://dx.doi.org/10.1186/s12951-020-00720-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7648390PMC
November 2020

Novel physiologic nomogram discriminates symptom outcome in patients with erosive esophagitis.

Esophagus 2021 Apr 6;18(2):407-415. Epub 2020 Nov 6.

Department of Gastroenterology, The First Affiliated Hospital of Sun Yat-sen University, 58 Zhongshan II Road, Guangzhou, 510080, Guangdong Province, China.

Background And Aim: Most of patients with erosive esophagitis (EE) are of LA grade A&B with low reflux burden, therefore require further esophageal function tests (EFTs). One-third of them respond poorly to pump proton inhibitor (PPI) treatment. The aim was to establish and validate a physiologic nomogram to discriminate symptom outcome to PPI treatment in patients with EE.

Methods: A total of 79 EE patients with heartburn who underwent EFTs and received PPI therapy were randomly assigned into a training set (n = 55) and a validation set (n = 24). Clinical data including physiologic parameters from EFTs were collected. Significant factors for the positive symptomatic outcome were identified using logistic regression analysis. Physiologic signature was developed using the least absolute shrinkage and selection operator algorithm. The nomogram was established by combining significant factors and physiologic signature, and its performance was evaluated and validated in the training and validation set. The clinical value of the nomogram was measured by decision curve analysis.

Results: Significant factors for positive symptomatic response to PPI treatment were identified as follows: acid exposure time, total number of reflux episodes, and two novel metrics including mean nocturnal baseline impedance and post-reflux swallow-induced peristaltic wave index. The nomogram which incorporated both significant factors and physiologic signature demonstrated good performance in the training and validation sets [C-index: 0.938 (95% CI 0.882-0.995); 0.839 (95% CI 0.678-0.995), respectively]. Decision curves showed significant clinical usefulness.

Conclusion: The first physiologic nomogram was developed to discriminate the individualized response to PPI therapy among EE patients.
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http://dx.doi.org/10.1007/s10388-020-00793-yDOI Listing
April 2021

Kinesin family member 3A inhibits the carcinogenesis of non-small cell lung cancer and prolongs survival.

Oncol Lett 2020 Dec 10;20(6):348. Epub 2020 Oct 10.

Department of Pulmonary and Critical Care Medicine, Qilu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China.

Kinesin family member 3A (KIF3A) plays a crucial role in the carcinogenesis of different types of human cancer. The present study aimed to identify the role of KIF3A in the carcinogenesis of non-small cell lung cancer (NSCLC). KIF3A protein expression was determined in 163 patients with NSCLC using immunohistochemistry staining. The prognosis of patients with NSCLC was determined using Kaplan-Meier survival and Cox regression analyses. The function of KIF3A on the carcinogenesis and metastasis of NSCLC was determined . Furthermore, a protein-protein interaction (PPI) network of KIF3A was constructed and the potential interacting molecules were identified using bioinformatic analysis. The protein expression levels of KIF3A were significantly lower in the NSCLC tissues compared with that in the adjacent tissues, and low KIF3A expression level was associated with unfavorable survival outcomes in patients with NSCLC. Furthermore, KIF3A knockdown increased proliferation, invasion and metastasis, and inhibited apoptosis of NSCLC cells. KIF3A was demonstrated to interact with intraflagellar transport 57 (IFT57) in the PPI network. In addition, validation analyses indicated that KIF3A mRNA expression levels were positively correlated with IFT57 mRNA expression levels in clinical NSCLC samples and NSCLC cell lines. Taken together, the results of the present study suggested that KIF3A is a key tumor suppressor gene for carcinogenesis and metastasis of NSCLC, it may also function as a biomarker and interacts with IFT57 in the progression of NSCLC.
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http://dx.doi.org/10.3892/ol.2020.12211DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7586287PMC
December 2020

Anti-oncogene PTPN13 inactivation by hepatitis B virus X protein counteracts IGF2BP1 to promote hepatocellular carcinoma progression.

Oncogene 2021 01 13;40(1):28-45. Epub 2020 Oct 13.

Department of Hepatobiliary Surgery, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, 510120, Guangzhou, China.

Hepatitis B x protein (HBx) affects cellular protein expression and participates in the tumorigenesis and progression of hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC). Metabolic reprogramming contributed to the HCC development, but its role in HBV-related HCC remains largely unclear. Tyrosine-protein phosphatase nonreceptor type 13 (PTPN13) is a significant regulator in tumor development, however, its specific role in hepatocarcinogenesis remains to be explored. Here, we found that decreased PTPN13 expression was associated with HBV/HBx. Patients with low PTPN13 expression showed a poor prognosis. Functional assays revealed that PTPN13 inhibited proliferation and tumorigenesis in vitro and in vivo. Further mechanistic studies indicated that HBx inhibited PTPN13 expression by upregulating the expression of DNMT3A and interacting with DNMT3A. Furthermore, we found that DNMT3A bound to the PTPN13 promoter (-343 to -313 bp) in an epigenetically controlled manner associated with elevated DNA methylation and then inhibited PTPN13 transcription. In addition, we identified IGF2BP1 as a novel PTPN13-interacting gene and demonstrated that PTPN13 influences c-Myc expression by directly and competitively binding to IGF2BP1 to decrease the intracellular concentration of functional IGF2BP1. Overexpressing PTPN13 promoted c-Myc mRNA degradation independent of the protein tyrosine phosphatase (PTP) activity of PTPN13. Importantly, we discovered that the PTPN13-IGF2BP1-c-Myc axis was important for cancer cell growth through promoting metabolic reprogramming. We verified the significant negative correlations between PTPN13 expression and c-Myc, PSPH, and SLC7A1 expression in clinical HCC tissue samples. In summary, our findings demonstrate that PTPN13 is a novel regulator of HBV-related hepatocarcinogenesis and may play an important role in HCC. PTPN13 may serve as a prognostic marker and therapeutic target in HBV-related HCC patients.
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http://dx.doi.org/10.1038/s41388-020-01498-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7790756PMC
January 2021

Post-reflux swallow-induced peristaltic wave (PSPW): physiology, triggering factors and role in reflux clearance in healthy subjects.

J Gastroenterol 2020 Dec 29;55(12):1109-1118. Epub 2020 Sep 29.

Wingate Institute of Neurogastroenterology, Blizard Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, 26 Ashfield Street, London, E1 2AJ, UK.

Background And Aims: The underlying physiology of post-reflux swallow-induced peristaltic wave (PSPW) is unclear. We aimed to: 1) calculate the probability of a random association between reflux and PSPW; 2) characterize factors that could underlie triggering of PSPW and 3) assess the chemical clearance effect of PSPW in healthy asymptomatic subjects.

Methods: A total of 251 impedance-pH tracings from healthy asymptomatic subjects were analysed. Twenty consecutive tracings from this pool with 20-40 reflux episodes/24 h and a PSPW index higher than 50% were separately analyzed to evaluate the probability of a random association between reflux and PSPW. The characteristics of reflux episodes followed by a PSPW were compared with those not associated with PSPW.

Results: A mean time interval of 29.3 s between a reflux episode and the first swallow captured 71% of total reflux episodes, and 67% of accompanying swallows were non-random. Compared to reflux without PSPW, reflux episodes with PSPW were more frequently acidic (P = 0.048), mixed with gas (P < 0.0001), of high proximal extent (P < 0.0001), while awake (P < 0.0001), and with shorter chemical clearance time (P = 0.040). High proximal extent, gas presence and occurring while awake were independent factors associated with PSPW (P < 0.0001).

Conclusion: Using a time window between reflux and PSPW of around 30 s, the probability of a chance association is around 30%. Reflux episodes with high proximal extent, containing gas and occurring while awake are important factors associated with PSPW in healthy subjects. Reflux episodes with PSPW have a shorter chemical clearance time.
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http://dx.doi.org/10.1007/s00535-020-01732-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7679330PMC
December 2020

Hydrogen inhalation alleviates nonalcoholic fatty liver disease in metabolic syndrome rats.

Mol Med Rep 2020 Oct 28;22(4):2860-2868. Epub 2020 Jul 28.

Taishan Institute for Hydrogen Biomedicine, Tai'an, Shandong 271000, P.R. China.

Hydrogen exhibits therapeutic and preventive effects against various diseases. The present study investigated the potential protective effect and dose‑dependent manner of hydrogen inhalation on high fat and fructose diet (HFFD)‑induced nonalcoholic fatty liver disease (NAFLD) in Sprague‑Dawley rats. Rats were randomly divided into four groups: i) Control group, regular diet/air inhalation; ii) model group, HFFD/air inhalation; iii) low hydrogen group, HFFD/4% hydrogen inhalation; and iv) high hydrogen group, HFFD/67% hydrogen inhalation. After a 10‑week experiment, hydrogen inhalation ameliorated weight gain, abdominal fat index, liver index and body mass index of rats fed with HFFD and lowered the total area under the curve in an oral glucose tolerance test. Hydrogen inhalation also ameliorated the increase in liver lipid content and alanine transaminase and aspartate transaminase activities. Liver histopathologic changes evaluated with hematoxylin and eosin as well as Oil Red O staining revealed lower lipid deposition in hydrogen inhalation groups, consistent with the decrease in the expression of the lipid synthesis gene SREBP‑1c. The majority of the indicators were affected following treatment with hydrogen in a dose‑dependent manner. In conclusion, hydrogen inhalation may play a protective role by influencing the general state, lipid metabolism parameters, liver histology and liver function indicators in the rat model of metabolic syndrome with NAFLD.
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http://dx.doi.org/10.3892/mmr.2020.11364DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7453621PMC
October 2020
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