Publications by authors named "Mengyao Zhang"

48 Publications

A pH-sensitive drug delivery system based on hyaluronic acid co-deliver doxorubicin and aminoferrocene for the combined application of chemotherapy and chemodynamic therapy.

Colloids Surf B Biointerfaces 2021 Apr 7;203:111750. Epub 2021 Apr 7.

School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan, 430070, PR China. Electronic address:

Doxorubicin is a broad-spectrum antineoplastic drug used in tumor therapy, its clinical application is limited by side effects on normal tissues. In this article, a pH-responsive drug delivery system (NPs(DOX/AFc)) with co-delivers doxorubicin (DOX) and aminoferrocene (AFc) was prepared by a two-step synthesis method including the oxidation of hyaluronic acid and Schiff base reaction. NPs(DOX/AFc) can be used in combination therapy of chemodynamic therapy (CDT) and chemotherapy (CT), thus the dosage of the chemotherapeutic drug DOX was reduced. The drug release behavior of NPs(DOX/AFc) in vitro showed that acid-responsive drug releases under the endosomal/lysosomal environment were 56.5 % of DOX and 61.8 % of AFc. In vitro toxicity experiments showed that DOX and AFc had synergistic effects (CI = 0.878). The results of intracellular ROS measurement and the mitochondrial membrane potential analysis showed that in tumor cells NPs(DOX/AFc) induced more production of reactive oxygen species and more loss of the mitochondrial membrane potential. In short, this co-delivery system based on polymer prodrugs provides a new idea for the combined application of CT and CDT.
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http://dx.doi.org/10.1016/j.colsurfb.2021.111750DOI Listing
April 2021

The Peculiar Clinical Symptoms and Treatment of Limbic Encephalitis Associated with AMPA Receptor Antibody.

Eur Neurol 2021 Apr 15:1-6. Epub 2021 Apr 15.

Department of Neurology, Xuanwu Hospital, Capital Medical University, Beijing, China.

Alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) antibody-related diseases are very rare in autoimmune neurological diseases. We collected and analyzed the data of 3 patients with peculiar clinical manifestation positive for AMPA2-R antibody in the Department of Neurology, Xuanwu Hospital, Capital Medical University. In our reported case series, 2 patients were male and 1 was female. The initial clinical features of 3 patients were all consistent with an encephalopathy predominantly involving the limbic system. Interestingly, as the disease continues to advance, case 1 presented with limb paralysis, case 2 developed typical cerebellar ataxia, and case 3 had symptoms of autonomic instability. These 3 types of clinical features were very rare in patients with AMPAR-Ab. After immunotherapy, cases 1 and 3 responded well and case 2 was not responsive. During the follow-up, residual memory loss of cases 1 and 3 improved gradually, and they returned to work. To eliminate the influence of the presence of other pathogenic antibodies, we analyzed the available individual clinical information of 37 patients with the single AMPAR-Ab by systematic literature review. A majority of patients had sudden short-term memory loss as the initial symptom and developed limbic encephalitis. Residual memory deficit was the most common symptom after discharge. The combination of at least 2 types of immunotherapy was recommended as the first-line treatment, and patients would benefit from the tumor screening. In addition, compared with the patients positive for single AMPAR-Ab, the patients with both AMPAR-Ab and other antibodies had a higher risk of malignant tumor and might have a poor therapeutic response, which led to a poor prognosis.
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http://dx.doi.org/10.1159/000515592DOI Listing
April 2021

A pH-sensitive oxidized-dextran based double drug-loaded hydrogel with high antibacterial properties.

Int J Biol Macromol 2021 Mar 30;182:385-393. Epub 2021 Mar 30.

School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, China. Electronic address:

Delayed healing or non-healing of wounds caused by bacterial infection is still a difficult medical problem. Nowadays, the topical application of antibiotics is a common treatment for infections. However, subinhibitory concentrations or high dose of antibiotics leads to the antibacterial effect counterproductive. So it's necessary to put forward an on-demand drug delivery to solve this tough issue. In this paper, a pH-responsive hydrogel was prepared by oxidized dextran (Dex-CHO), sulfadiazine (SD) and tobramycin (TOB). The hydrogel was designed by the environment in the early immature stage of biofilm (pH 5.0). Schiff bases can release drugs in slightly acidic environment. The hydrogel showed injectable, pH-sensitive drug release, and great biocompatibility. Released SD and TOB exhibited a synergistic effect therefore the hydrogel showed high antibacterial activity. This study provides an easy and promising strategy to develop smart hydrogels that aim at topical administration of antibiotics and come up with a new treatment of local bacterial infections.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.03.169DOI Listing
March 2021

A donor-π-acceptor aggregation-induced emission compound serving as a portable fluorescent sensor for detection and differentiation of methanol and ethanol in the gas phase.

Spectrochim Acta A Mol Biomol Spectrosc 2021 May 1;252:119515. Epub 2021 Feb 1.

Department of Chemistry, Capital Normal University, Beijing 100048, China. Electronic address:

The design strategy of aggregation-induced emission (AIE) fluorophores with donor-π-acceptor (D-π-A) conjugation structure has greatly contributed to the development of luminescent materials and devices, including volatile organic compounds (VOCs) sensors. In this work, a D-π-A fluorophore DEBAB was synthesized, showing both AIE and intramolecular charge transfer (ICT) properties as confirmed by spectroscopic data and quantum chemical calculations. Furthermore, there is notable emission-enhancement when DEBAB is exposed to small-molecule alcohols, such as methanol and ethanol. Based on this phenomenon, a portable film sensor was fabricated, capable of detecting methanol and ethanol in gas phase, with detection limit (DL) as low as 8.02 ppm. Our systematic investigation suggests that hydrogen-bonding may be formed between DEBAB and alcohols, intensifying the AIE efficacy while influencing the ICT process. This working mechanism is supported by density functional theory (DFT) calculations including electrostatic potential mapping and molecular total energy. In addition, a sensor array was fabricated on a cellulose paper strip, showing different levels of emission changing in response to alcohols. Thus the detection and differentiation of methanol and ethanol are enabled.
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http://dx.doi.org/10.1016/j.saa.2021.119515DOI Listing
May 2021

Carrying out pseudo dual nucleic acid detection from sample to visual result in a polypropylene bag with CRISPR/Cas12a.

Biosens Bioelectron 2021 Apr 19;178:113001. Epub 2021 Jan 19.

Key Laboratory of Microbiol Technology and Bioinformatics of Zhejiang Province, Zhejiang Institute of Microbiology, Hangzhou, 310012, China; Hangzhou Digital-Micro Biotech Co., Ltd., Hangzhou, 311215, China. Electronic address:

Amplification-based nucleic acid detection is widely employed in food safety, medical diagnosis and environment monitoring. However, conventional nucleic acid analysis has to be carried out in laboratories because of requiring expensive instruments and trained personnel. If people could do nucleic acid detection at home by themselves, the application of nucleic acid detection would be greatly accelerated. We herein reported a polypropylene (PP) bag-based method for convenient detection of nucleic acids in the oil-sealed space. The PP bag has three chambers which are responsible for lysis, washing and amplification/detection, respectively. After adding sample, nucleic acids are adsorbed on magnetic particles (MPs) and moved into these three chambers successively through immiscible oil channel by an external magnet. Combined with isothermal amplification, the PP bag can be incubated in a water bath or milk warmer and acted as a reaction tube. With highly specific CRISPR technology, Salmonella typhimurium (St) and SARS-CoV-2 can be visually detected in these PP bags within 1 h, indicating its potential household application. To further improve the reliability of nucleic acid testing at home, a logic decision method is introduced by detecting both target and endogenous reference gene. Positive/negative/invalid detection result can be obtained by chronologically adding the CRISPR reagents of target and endogenous reference gene. We anticipate that this PP bag can provide a novel toolkit for nucleic acid detection in people's daily life.
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http://dx.doi.org/10.1016/j.bios.2021.113001DOI Listing
April 2021

Involvement of circHIPK3 in the pathogenesis of diabetic cardiomyopathy in mice.

Diabetologia 2021 Mar 4;64(3):681-692. Epub 2021 Jan 4.

Department of Cardiology, The Second Affiliated Hospital of Soochow University, Suzhou, China.

Aims/hypothesis: In a mouse model of diabetic cardiomyopathy (DCM) the expression of the circular RNA circHIPK3 was found to be significantly increased. This study aimed to discover the molecular mechanisms linking circHIPK3 to the pathogenesis of DCM.

Methods: The diabetic mouse model was established by i.p. injection of streptozotocin, which led to the development of DCM. Echocardiographic measurements were used to evaluate cardiac structure and function, and histological staining was applied to detect myocardial fibrosis in mice. 5-Ethynyl-2'-deoxyuridine incorporation was performed to determine cell proliferation and RNA fluorescent in situ hybridisation was employed to examine circHIPK3 expression in cardiac fibroblasts. RNA immunoprecipitation and luciferase reporter assay were conducted to explore the pathological mechanism of circHIPK3 in myocardial fibrosis.

Results: Knockdown of circHIPK3 was found to attenuate myocardial fibrosis and enhance cardiac function in DCM mice. In addition, silencing of circHIPK3 could suppress proliferation of cardiac fibroblasts treated with angiotensin II. Furthermore, RNA immunoprecipitation and luciferase reporter assay revealed a circHIPK3-miR-29b-3p-Col1a1-Col3a1 regulatory network in the pathogenesis of myocardial fibrosis.

Conclusions/interpretation: circHIPK3 contributes to increased myocardial fibrosis during DCM by functioning as a competing endogenous RNA that upregulates Col1a1 and Col3a1 expression through suppressing miR-29b-3p.
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http://dx.doi.org/10.1007/s00125-020-05353-8DOI Listing
March 2021

A novel human anti-TIGIT monoclonal antibody with excellent function in eliciting NK cell-mediated antitumor immunity.

Biochem Biophys Res Commun 2021 01 16;534:134-140. Epub 2020 Dec 16.

Department of Urology, Xijing Hospital, Fourth Military Medical University, Xi'an, China. Electronic address:

TIGIT is an emerging novel checkpoint target that is expressed on both tumor-infiltrating T cells and NK cells. Some current investigational antibodies targeting TIGIT have also achieved dramatic antitumor efficacy in late clinical research. Most recently, the relevance of NK cell-associated TIGIT signaling pathway to tumors' evasion of the immune system has been clearly revealed, which endows NK cells with a pivotal role in the therapeutic effects of TIGIT blockade. In this article, we describe a novel anti-TIGIT monoclonal antibody, AET2010, which was acquired from a phage-displayed human single-chain antibody library through a cell panning strategy. With emphasis on its regulation of NK cells, we confirmed the excellent ex vivo and in vivo antitumor immunity of AET2010 mediated by the NK-92MI cells. Intriguingly, our work also revealed that AET2010 displays a lower affinity but parallel avidity and activity relative to MK7684, an investigational monoclonal antibody from MSD, implying a reasonable balance of potency and potential side effects for AET2010. Together, these results are promising and warrant further development of AET2010.
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http://dx.doi.org/10.1016/j.bbrc.2020.12.013DOI Listing
January 2021

Etv5 safeguards trophoblast stem cells differentiation from mouse EPSCs by regulating fibroblast growth factor receptor 2.

Mol Biol Rep 2020 Dec 6;47(12):9259-9269. Epub 2020 Nov 6.

College of Veterinary Medicine, Shaanxi Center of Stem Cells Engineering and Technology, Northwest A&F University, 712100, Yangling, China.

Previous studies have demonstrated that transcription factor Etv5 plays an important role in the segregation between epiblast and primitive endoderm at the second fate decision of early embryo. However, it remains elusive whether Etv5 functions in the segregation between inner cell mass and trophectoderm at the first cell fate decision. In this study, we firstly generated Etv5 knockout mouse embryonic stem cells (mESCs) by CRISPR/Cas9, then converted them into extended potential stem cells (EPSCs) by culturing the cells in small molecule cocktail medium LCDM (LIF, CHIR99021, (S)-(+)-dimethindene maleate, minocycline hydrochloride), and finally investigated their differentiation efficiency of trophoblast stem cells (TSCs). The results showed that Etv5 knockout significantly decreased the efficiency of TSCs (CDX2) differentiated from EPSCs. In addition, Etv5 knockout resulted in higher incidence of the differentiated cells with tetraploid and octoploid than that from wild type. Mechanistically, Etv5 was activated by extracellular-signal-regulated kinase (ERK) signaling pathway; in turn, Etv5 had a positive feedback on the expression of fibroblast growth factor receptor 2 (FGFR2) which lies upstream of ERK. Etv5 knockout decreased the expression of FGFR2, whose binding with fibroblast growth factor 4 was essentially needed for TSCs differentiation. Collectively, the findings in this study suggest that Etv5 is required to safeguard the TSCs differentiation by regulating FGFR2 and provide new clues to understand the specification of trophectoderm in vivo.
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http://dx.doi.org/10.1007/s11033-020-05969-4DOI Listing
December 2020

Nonclinical safety, tolerance and pharmacodynamics evaluation for meplazumab treating chloroquine-resistant .

Acta Pharm Sin B 2020 Sep 30;10(9):1680-1693. Epub 2020 Jun 30.

College of Life Science and Bioengineering, School of Science, Beijing Jiaotong University, Beijing 100044, China.

Meplazumab is an anti-CD147 humanized IgG2 antibody. The purpose of this study was to characterize the nonclinical safety, tolerance and efficacy evaluation of meplazumab treating chloroquine resistant . Meplazumab was well tolerated in repeat-dose toxicology studies in cynomolgus monkeys. No observed adverse effect level was 12 mg/kg. No difference between genders in the primary toxicokinetic parameters after repeat intravenous injection of meplazumab. No increased levels of drug exposure and drug accumulation were observed in different gender and dose groups. Meplazumab had a low cross-reactivity rate in various tissues and did not cause hemolysis or aggregation of red blood cells. The biodistribution and excretion results indicated that meplazumab was mainly distributed in the plasma, whole blood, and hemocytes, and excreted in the urine. Moreover, meplazumab effectively inhibited the parasites from invading erythrocytes in humanized mice in a time-dependent manner and the efficacy is superior to that of chloroquine. All these studies suggested that meplazumab is safe and well tolerated in cynomolgus monkeys, and effectively inhibits from invading into human red blood cells. These nonclinical data facilitated the initiation of an ongoing clinical trial of meplazumab for antimalarial therapy.
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http://dx.doi.org/10.1016/j.apsb.2020.06.011DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7564037PMC
September 2020

Morphological parameters of fourth lumbar spinous process palpation: a three-dimensional reconstruction of computed tomography.

J Orthop Surg Res 2020 Jun 22;15(1):227. Epub 2020 Jun 22.

Academician Workstation in Luzhou, Luzhou, China.

Background: The localization of lumbar fourth spinous process (L4-SP) is an important anatomical landmark, and identifying its accurate position is essential for the diagnosis and treatment of waist diseases.

Methods: Five hundred participants were scanned with positive and lateral computed tomography (CT), which aimed to clarify anatomic characteristics of L4-SP. Anatomical parameters of the surface localization of L4-SP were measured and recorded through a three-dimensional (3D) reconstruction.

Results: Five hundred participants were classified into three types according to the position of BC with the iliac spine. There are just 266 that the line between the highest point of the iliac spine on both sides located on L4-SP (type I, 53.20%), 16 above L4-SP (type II, 3.20%), and 218 below L4-SP (type III, 43.60%). BC in type I (15.92 ± 1.30 mm) is longer than type III (15.56 ± 1.32 mm). While the angle combined with AB and BC is different in the three groups, the angle in type I (173.00 ± 4.83°) is larger than that in type II (164.69 ± 5.50°) and type III (159.45 ± 8.39°). Other measurements were not found any significant differences between above.

Conclusion: The traditional palpation for L4-SP is not absolutely exact. The accuracy rate is only 53.20%, and the errors may cause serious consequences.
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http://dx.doi.org/10.1186/s13018-020-01750-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7309970PMC
June 2020

Antibodies in the breast milk of a maternal woman with COVID-19.

Emerg Microbes Infect 2020 Dec;9(1):1467-1469

Beijing Institute of Biotechnology, Beijing, People's Republic of China.

A maternal woman was positive for SARS-CoV-2 tested in throat swabs but negative tested in other body fluids, and she had IgG and IgA detected in breast milk. Her infant negative for SARS-CoV-2 at birth had elevated IgG in serum but quickly decayed. These findings suggest that breastfeeding might have the potential benefit to the neonates.
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http://dx.doi.org/10.1080/22221751.2020.1780952DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7475804PMC
December 2020

Two water-soluble fluorescence probes based on 5(6)-carboxyl rhodamine for Cu imaging in living cells.

Luminescence 2020 Nov 2;35(7):1101-1108. Epub 2020 Jun 2.

College of Chemistry and Materials Science, Fujian Normal University, Fuzhou, China.

Two novel water-soluble fluorescence probes T1 and T2 based on 5(6)-carboxyl rhodamine were designed and synthesized using a regioselective reaction. The probes exhibited highly selective and sensitive recognition toward Cu over other metal ions in acetonitrile/Tris-HCl buffer solution (2:98, v/v; pH 7.4). Detection limits were 0.4 μM for T1 and 4.50 μM for T2 based on fluorescence titration analysis. Furthermore, probe T1 was successfully applied in cell imaging experiments to monitor Cu in cells.
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http://dx.doi.org/10.1002/bio.3822DOI Listing
November 2020

Overcurrent Electrodeposition of Fractal Plasmonic Black Gold with Broad-Band Absorption Properties for Excitation-Immune SERS.

ACS Omega 2020 Apr 2;5(14):8293-8298. Epub 2020 Apr 2.

State Key Laboratory for Physical Chemistry of Solid Surfaces and Department of Chemistry, Xiamen University, Xiamen 361005, China.

The dependence of plasmon resonance on the size, shape, and interparticle spacing of single, isolated nanostructures inherently limits their light-harvesting capability to a narrow spectral band. Here, we report a facile overcurrent electrodeposition strategy to prepare fractal plasmonic black gold (B-Au) with broad-band absorption properties (over 80% throughout the range of 300-1800 nm). The broad-band absorption properties are attributed to the excitation of multiple plasmons in the B-Au, which results in strong light-matter interaction over a broad-band spectral window. Consequently, the B-Au can produce strong broad-band surface-enhanced Raman scattering (SERS) regardless of the excitation light used. These findings demonstrate that the fractal B-Au allows efficient utilization of broad spectral photons and opens up exciting opportunities for highly sensitive SERS detection, photocatalysis, and photovoltaic devices.
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http://dx.doi.org/10.1021/acsomega.0c00698DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7161050PMC
April 2020

Selective endpoint visualized detection of Vibrio parahaemolyticus with CRISPR/Cas12a assisted PCR using thermal cycler for on-site application.

Talanta 2020 Jul 11;214:120818. Epub 2020 Feb 11.

Key Laboratory of Microbiol Technology and Bioinformatics of Zhejiang Province, Zhejiang Institute of Microbiology, Hangzhou, 310012, China; NMPA Key Laboratory for Testing and Risk Warning of Pharmaceutical Microbiology, Zhejiang Institute of Microbiology, Hangzhou, 310012, China. Electronic address:

Vibrio parahaemolyticus is a major cause of seafood-associated food poisoning. It is of great significance to develop an accurate, simple and cost-effective method to identify infected seafood, especially for on-site application. Polymerase chain reaction (PCR) remains the golden standard for nucleic acid detection. But traditional methods heavily reply on sophisticated instrument and specialized operators, which limits the application for on-site detections. Here we developed a novel, specific and visualized detection method for PCR based on CRISPR/Cas12a system. On a low-cost thermal cycler, amplification reaction can be conducted easily. The CRISPR/Cas12a system was specifically designed to evaluate amplicons, eliminating false positive results. Besides the negative samples remained colorless, the positive samples generated obvious green fluorescence, which could be easily distinguished by the naked eye using a homemade UV device. The presented detection method was verified by detecting shrimp samples. The limit of detection is 1.02 × 10 copies/μL. This presented method provided a new strategy for specific endpoint detection of PCR and advanced its application in field for food safety assurance.
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http://dx.doi.org/10.1016/j.talanta.2020.120818DOI Listing
July 2020

The discovery of dynamic chiral anomaly in a Weyl semimetal NbAs.

Nat Commun 2020 Mar 6;11(1):1259. Epub 2020 Mar 6.

State Key Laboratory of Surface Physics and Department of Physics, Fudan University, Shanghai, 200433, China.

The experimental discovery of Weyl semimetals offers unprecedented opportunities to study Weyl physics in condensed matters. Unique electromagnetic response of Weyl semimetals such as chiral magnetic effect has been observed and presented by the axial θ E · B term in electromagnetic Lagrangian (E and B are the electric and magnetic field, respectively). But till now, the experimental progress in this direction in Weyl semimetals is restricted to the DC regime. Here we report experimental access to the dynamic regime in Weyl semimetal NbAs by combining the internal deformation potential of coupled phonons with applied static magnetic field. While the dynamic E · B field is realized, it produces an anomalous phonon activity with a characteristic angle-dependence. Our results provide an effective approach to achieve the dynamic regime beyond the widely-investigated DC limit which enables the coupling between the Weyl fermions and the electromagnetic wave for further study of novel light-matter interactions in Weyl semimetals.
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http://dx.doi.org/10.1038/s41467-020-14749-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7060315PMC
March 2020

Machine learning-guided design and development of multifunctional flexible Ag/poly (amic acid) composites using the differential evolution algorithm.

Nanoscale 2020 Feb 4;12(6):3988-3996. Epub 2020 Feb 4.

Shanghai Key Laboratory of Multidimensional Information Processing, East China Normal University, 500 Dongchuan Road, 200241, Shanghai, China. and Shanghai Institute of Intelligent Electronics & Systems, Fudan University, Shanghai 200433, China.

The development of flexible composites is of great significance in the flexible electronic field. In combination with machine learning technology, the introduction of artificial intelligence to flexible materials design, synthesis, characterization and application research will greatly promote the flexible materials research efficiency. In this study, the back propagation (BP) neural network based on the differential evolution (DE) algorithm was applied to determine the electrical properties of the flexible Ag/poly (amic acid) (PAA) composite structure and to develop flexible materials for its different applications. In the machine learning model, the concentration of PAA, the ion exchange time of AgNO, and the concentration and reduction time of NaBH are set as input parameters, and the product of the sheet resistance of the Ag/PAA film and the processing time are set as output information. To overcome the situation whereby the BP neural network solution process could fall into the local optimum, the initial threshold and the weight of the BP neural network and the data import model are optimized by the DE algorithm. Utilizing 1077 learning samples and 49 predictive samples, a machine learning model with very high accuracy was established and relative errors of predictions less than 1.96% were achieved. In terms of this model, the optimized fabrication conditions of the Ag/PAA composites, which are suitable for strain sensors and electrodes, were predicted. To identify the availability and applicability of the proposed algorithm, a strain gauge sensor, a triboelectric nanogenerator (TENG) and a capacitive pressure sensor array were fabricated successfully using the optimized process parameters. This work shows that machine learning can be used to quickly optimize the process and provide guidance for material and process design, which is of significance for the development of flexible materials and devices.
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http://dx.doi.org/10.1039/c9nr09146gDOI Listing
February 2020

One-step synthesis of rhodamine-based Fe fluorescent probes via Mannich reaction and its application in living cell imaging.

Spectrochim Acta A Mol Biomol Spectrosc 2020 Apr 23;231:118105. Epub 2020 Jan 23.

College of Chemistry and Materials Science, Fujian Normal University, Fuzhou 350007, China; Fujian Provincial University Engineering Research Center of Industrial Biocatalysis, Fujian Normal University, Fuzhou 350117, China. Electronic address:

Four rhodamine-based fluorescent probes M1-M4 were synthesized in one step using Mannich reaction. The Mannich reaction based approach has the advantages of simplicity, good yield and excellent atomic economy. The structures were determined by H NMR, C NMR, IR and HRMS. The probe M3 as a representative compound was characterized by single-crystal X-ray analyses. The fluorescence and absorbance spectra research of the probes demonstrated that they could be used as Fe-selective fluorescent probes with good sensitivity, excellent linearity, and outstanding anti-interference in acetonitrile/Tris-HCl buffer solution (3:7, V/V; pH = 7.4). Moreover, confocal laser scanning microscopy experiments have proven that the probe M3 was successfully used for fluorescence imaging in MCF-7 cells.
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http://dx.doi.org/10.1016/j.saa.2020.118105DOI Listing
April 2020

Visual detection for nucleic acid-based techniques as potential on-site detection methods. A review.

Anal Chim Acta 2020 Feb 26;1099:1-15. Epub 2019 Nov 26.

College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, China; Key Laboratory of on Site Processing Equipment for Agricultural Products, Ministry of Agriculture, Hangzhou, 310058, China. Electronic address:

Nucleic acid-based techniques could achieve highly sensitive detection by amplifying template molecules to millions of folds. It has been one of the most valued analytical methods and is applied in many detection fields, such as diagnosis of infectious diseases, food safety assurance and so on. Nucleic acid-based techniques consist of three steps: nucleic acid extraction, amplification, and product detection. Among them, the detection step plays a vital role because it shows the results directly. As the trend of detection is simple, rapid and instrument-free, it is of necessity to carry out visual detection, where the result read-out could be visible and distinguished by the naked eye. In this critical review, advanced visual detection methods are summarized and discussed in detail, aiming to promote the potential application in on-site detection.
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http://dx.doi.org/10.1016/j.aca.2019.11.056DOI Listing
February 2020

A novel cholesterol conjugated fluorescence probe for Cu detection and bioimaging in living cells.

Spectrochim Acta A Mol Biomol Spectrosc 2020 Feb 13;227:117530. Epub 2019 Sep 13.

College of Chemistry and Materials Science, Fujian Normal University, Fuzhou, 350007, China; Fujian Provincial University Engineering Research Center of Industrial Biocatalysis, Fujian Normal University, Fuzhou, 350117, China. Electronic address:

A cholesterol conjugated fluorescence probe T was designed and synthesized. The probe T can be used for recognition of Cu by the absorption spectrum, fluorescence spectrum, and naked eyes respectively in aqueous solution. The cell imaging experiments showed that the probe has good membrane permeability and a huge potentiality for the detection of Cu in living cells.
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http://dx.doi.org/10.1016/j.saa.2019.117530DOI Listing
February 2020

Paper-Based ZnS:Cu Alternating Current Electroluminescent Devices for Current Humidity Sensors with High-Linearity and Flexibility.

Sensors (Basel) 2019 Oct 23;19(21). Epub 2019 Oct 23.

Shanghai Key Laboratory of Multidimensional Information Processing, East China Normal University, 500 Dongchuan Road, Shanghai 200241, China.

Humidity sensors are indispensable for various electronic systems and instrumentations. To develop a new humidity sensing mechanism is the key for the next generation of sensor technology. In this work, a novel flexible paper-based current humidity sensor is proposed. The developed alternating current electroluminescent devices (ACEL) consist of the electroless plating Ni on filter paper and silver nanowires (AgNWs) as the bottom and upper electrodes, and ZnS:Cu as the phosphor layer, respectively. The proposed humidity sensor is based on ACEL with the paper substrate and the ZnS:Cu phosphor layer as the humidity sensing element. The moisture effect on the optical properties of ACELs has been studied firstly. Then, the processing parameters of the paper-based ACELs such as electroless plated bottom electrode and spin-coated phosphor layer as a function of the humidity-sensitive characteristics are investigated. The sensing mechanism of the proposed sensor has been elucidated based on the Q ~ V analysis. The sensor exhibits an excellent linearity ( R 2 = 0.99965 ) within the humidity range from 20% to 90% relative humidity (RH) and shows excellent flexibility. We also demonstrate its potential application in postharvest preservation where the EL light is used for preservation and the humidity can be monitored simultaneously through the current.
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http://dx.doi.org/10.3390/s19214607DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6864801PMC
October 2019

Construction of Covalent-Organic Frameworks (COFs) from Amorphous Covalent Organic Polymers via Linkage Replacement.

Angew Chem Int Ed Engl 2019 Dec 22;58(49):17679-17683. Epub 2019 Oct 22.

Tianjin Key Laboratory of Structure and Performance for Functional Molecules, Key Laboratory of Inorganic-Organic Hybrid Functional Material Chemistry (Ministry of Education), College of Chemistry, Tianjin Normal University, Tianjin, 300387, P. R. China.

Covalent-organic frameworks (COFs) as porous crystalline materials show promising potential applications. However, developing facile strategies for the construction of COFs directly from amorphous covalent organic polymers (COPs) is still a great challenge. To this end, we report a novel approach for easy preparation of COFs from amorphous COPs through the linkage replacement under different types of reactions. Four COFs with high crystallinity and porosity were constructed via the linkage substitution of polyimide-linked COPs to imine-linked COFs as well as imine-linked COPs to polyimide-linked COFs. The realization of the linkage substitution would significantly expand the research scope of COFs.
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http://dx.doi.org/10.1002/anie.201911231DOI Listing
December 2019

Expression Pattern of and miR156-Targeted Genes Associated with Developmental Stages in .

Int J Mol Sci 2019 Jun 3;20(11). Epub 2019 Jun 3.

Fujian Provincial Key Laboratory of Plant Functional Biology, College of Horticulture, Fujian A & University, Fuzhou 350002, China.

Time to flower, a process either referring to juvenile-adult phase change or vegetative-reproductive transition, is strictly controlled by an intricate regulatory network involving at least both and the micro RNA (miR)156-regulated family members. Despite substantial progresses recently achieved in and other plant species, information regarding the involvement of these genes during orchid development and flowering competence is still limited. , a popular orchid species, exhibits a juvenile phase of at least three years. Here, through whole-genome mining and whole-family expression profiling, we analyzed the homologous genes of , miR156, and with special reference to the developmental stages. The family contains nine members; among them, transcribes abundantly in young and juvenile tissues but not in adult, contrasting with the low levels of others. We also found that mature miR156, encoded by a single locus, accumulated in large quantity in protocorms and declined by seedling development, coincident with an increase in transcripts of three of its targeted members, namely , , and . Moreover, among the seven predicted miR156-targeted , only was significantly expressed in adult plants and was associated with plant maturation. Our results might suggest that the juvenile phase change or maturation in this orchid plant likely involves both the repressive action of a -like pathway and the promotive effect from an -mediated mechanism.
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http://dx.doi.org/10.3390/ijms20112725DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6600168PMC
June 2019

A mechanochromic luminescent material with aggregation-induced emission: Application for pressure sensing and mapping.

Spectrochim Acta A Mol Biomol Spectrosc 2019 Sep 14;220:117125. Epub 2019 May 14.

Department of Chemistry, Capital Normal University, Beijing 100048, China. Electronic address:

In this study, we report a new compound, (E)-4-(((2-hydroxynaphthalen-1-yl)methylene)amino)-3-methylbenzoic acid (HNMB), which shows aggregation-induced emission property as well as intramolecular charge transfer (ICT) nature. In addition, it exhibits unique mechanochromic luminescence (MCL). The HNMB solid powder emits strong emission but shows quenching effect together with bathochromic-shift after grinding, suggesting a high contrast ratio up to 1420%. Through crystallographic analysis, the relationship between MCL nature and molecular packing mode is verified: Molecules in crystalline phase adopt the J-type coupling based on less overlapped π⋯π stacking, in which multiple intermolecular interactions mainly including C-H⋯π, C-H⋯O and hydrogen bonding, help to stabilize such packing mode. When these interactions are destructed by mechanical force, the packing would be disassembled, activating the MCL behavior. Such working mechanism only needs weak external force capable of destructing intermolecular interactions, rendering the MCL material highly sensitive to pressure. As a practical application, a film sensor for pressure detection is designed based on HNMB, which gives a linear relation between the emission intensity and the external pressure in a lower range. The detection limit of the film sensor is 27.24 Mpa, suggesting high sensitivity. In addition, pressure mapping with high contrast ratio is obtained by surface plot, making this pressure sensor a reliable candidate to be instrumented for various applications.
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http://dx.doi.org/10.1016/j.saa.2019.05.030DOI Listing
September 2019

Survival factor 1 contributes to the oxidative stress response and is required for full virulence of Sclerotinia sclerotiorum.

Mol Plant Pathol 2019 07 9;20(7):895-906. Epub 2019 May 9.

State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan City, 430070, P R China.

Sclerotinia sclerotiorum is a devastating necrotrophic fungal pathogen that infects over 400 species of plants worldwide. Reactive oxygen species (ROS) modulations are critical for the pathogenic development of S. sclerotiorum. The fungus applies enzymatic and non-enzymatic antioxidants to cope with the oxidative stress during the infection processes. Survival factor 1 was identified and characterized to promote survival under conditions of oxidative stress in Saccharomyes cerevisiae. In this research, a gene named SsSvf1 was predicted to encode a survival factor 1 homologue in S. sclerotiorum. SsSvf1 transcripts showed high expression levels in hyphae under oxidative stress. Silencing of SsSvf1 resulted in increased sensitivity to oxidative stress in culture and increased levels of intracellular ROS. Transcripts of SsSvf1 showed a dramatic increase during the initial stage of infection and the gene-silenced strains displayed reduced virulence on oilseed rape and Arabidopsis thaliana. Inhibition of plant ROS production partially restores virulence of SsSvf1 gene-silenced strains. SsSvf1 gene-silenced strains exhibited normal oxalate production, but were impaired in compound appressorium formation and cell wall integrity. The results suggest that SsSvf1 is involved in coping with ROS during fungal-host interactions and plays a crucial role in the pathogenicity of S. sclerotiorum.
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http://dx.doi.org/10.1111/mpp.12801DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6589728PMC
July 2019

Chimeric antigen receptor T cell targeting EGFRvIII for metastatic lung cancer therapy.

Front Med 2019 Feb 5;13(1):57-68. Epub 2019 Feb 5.

National Translational Science Center for Molecular Medicine, Xi'an, 710032, China.

Lung cancer is the most common incident cancer and the leading cause of cancer death. In recent years, the development of tumor immunotherapy especially chimeric antigen receptor T (CAR-T) cell has shown a promising future. Epidermal growth factor receptor variant III (EGFRvIII) is a tumor-specific mutation expressed in various types of tumors and has been detected in non-small cell lung cancer with a mutation rate of 10%. Thus, EGFRvIII is a potential antigen for targeted lung cancer therapy. In this study, CAR vectors were constructed and transfected into virus-packaging cells. Then, activated T cells were infected with retrovirus harvested from stable virus-producing single clone cell lines. CAR expression on the surfaces of the T cells was detected by flow cytometry and Western blot. The function of CAR-T targeting EGFRvIII was then evaluated. The EGFRvIII-CAR vector was successfully constructed and confirmed by DNA sequencing. A stable virus-producing cell line was produced from a single clone by limited dilution. The culture conditions for the cell line, including cell density, temperature, and culture medium were optimized. After infection with retrovirus, CAR was expressed on more than 90% of the T cells. The proliferation of CAR-T cells were induced by cytokine and specific antigen in vitro. More importantly, EGFRvIII-CART specifically and efficiently recognized and killed A549-EGFRvIII cells with an effector/target ratio of 10:1 by expressing and releasing cytokines, including perforin, granzyme B, IFN-γ, and TNF-α. The in vivo study indicated that the metastasis of A549-EGFRvIII cells in mice were inhibited by EGFRvIII-CART cells, and the survival of the mice was significantly prolonged with no serious side effects. EGFRvIII-CART showed significantly efficient antitumor activity against lung cancer cells expressing EGFRvIII in vivo and in vitro. Therefore, CAR-T targeting EGFRvIII is a potential therapeutic strategy in preventing recurrence and metastasis of lung cancer after surgery.
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http://dx.doi.org/10.1007/s11684-019-0683-yDOI Listing
February 2019

Degradable conductive self-healing hydrogels based on dextran-graft-tetraaniline and N-carboxyethyl chitosan as injectable carriers for myoblast cell therapy and muscle regeneration.

Acta Biomater 2019 01 5;84:180-193. Epub 2018 Dec 5.

Frontier Institute of Science and Technology, and State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an 710049, China.

Injectable conductive hydrogels have great potential as tissue engineering scaffolds and delivery vehicles for electrical signal sensitive cell therapy. In this work, we present the synthesis of a series of injectable electroactive degradable hydrogels with rapid self-healing ability and their potential application as cell delivery vehicles for skeletal muscle regeneration. Self-healable conductive injectable hydrogels based on dextran-graft-aniline tetramer-graft-4-formylbenzoic acid and N-carboxyethyl chitosan were synthesized at physiological conditions. The dynamic Schiff base bonds between the formylbenzoic acid and amine group from N-carboxyethyl chitosan endowed the hydrogels with rapid self-healing ability, which was verified by rheological test. Equilibrated swelling ratio, morphology, mechanical strength, electrochemistry and conductivity of the injectable hydrogels were fully investigated. The self-healable conductive hydrogels showed an in vivo injectability and a linear-like degradation behavior. Two different kinds of cells (C2C12 myoblasts and human umbilical vein endothelial cells (HUVEC)) were encapsulated in the hydrogels by self-healing effect. The L929 fibroblast cell culture results indicated the biocompatibility of the hydrogels. Moreover, the C2C12 myoblast cells were released from the conductive hydrogels with a linear-like profile. The in vivo skeletal muscle regeneration was also studied in a volumetric muscle loss injury model. All these data indicated that these biodegradable self-healing conductive hydrogels are potential candidates as cell delivery vehicles and scaffolds for skeletal muscle repair. STATEMENT OF SIGNIFICANCE: Injectable hydrogels with self-healing and electrical conductivity properties are excellent candidates as tissue-engineered scaffolds for myoblast cell therapy and skeletal muscle regeneration. The self-healing property of these hydrogels can prolong their lifespan. However, most of the reported conductive hydrogels are not degradable or do not have the self-healing ability. Herein, we synthesized antibacterial conductive self-healing hydrogels as a cell delivery carrier for cardiac cell therapy based on chitosan-grafted-tetraaniline hydrogels synthesized in our previous work. However, an acid solution was used to dissolve the polymers in that study, which may induce toxicity to cells. In this work, we synthesized a series of injectable electroactive biodegradable hydrogels with rapid self-healing ability composed of N-carboxyethyl chitosan (CECS) and dextran-graft-aniline oligomers, and these hydrogel precusor can dissolve in PBS solution of pH 7.4; we further demonstrated their potential application as cell delivery vehicles for skeletal muscle regeneration.
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http://dx.doi.org/10.1016/j.actbio.2018.12.008DOI Listing
January 2019

Effects of elevated CO on plant C-N-P stoichiometry in terrestrial ecosystems: A meta-analysis.

Sci Total Environ 2019 Feb 5;650(Pt 1):697-708. Epub 2018 Sep 5.

Institute of Mountain Hazards and Environment, Chinese Academy of Sciences, Chengdu 610041, China. Electronic address:

A substantial number of experiments have so far been carried out to study the response of the C-N-P stoichiometry of terrestrial plants to the rising CO level of the earth. However, there is a need of systematic evaluation for assessing the impact of the elevated CO on plant C-N-P stoichiometry. In the present investigation, a comprehensive meta-analysis involving 386 published reports and including 4481 observations has been carried out. The goal of the research was to determine the response of plants to their C-N-P stoichiometry due to elevated levels of global atmospheric CO. The results showed that rising CO altered the concentration of C (+2.19%, P < 0.05), N (-9.73%, P < 0.001) and P (-3.23%, P < 0.001) and C:N (+13.29%, P < 0.001) and N:P ratios (-7.32%, P < 0.0001). Overall, a slightly increasing trend in the C:P ratio (P > 0.05) in the plant was observed. However, plant leaf, shoot and herbaceous type of plants showed more sensitivity to rising CO. CO magnitude exhibited a positive effect (P < 0.05) on C:N ratio. Additionally, "CO acclimation" hypothesis as proposed by the authors of the current paper was also tested in the study. Results obtained, especially, show changes of C and N concentrations and C:P ratio to an obvious down-regulation for long-term CO fumigation. At spatial scales, a reduction of plant N concentration was found to be higher in the southern hemisphere. The CO enrichment methods affected the plant C-N-P stoichiometry. Compared to FACE (free-air CO enrichment), OTC (open top chamber) showed larger changes of C, N, P, and N:P. The results of the present study should, therefore, become helpful to offer a better understanding towards the response of the terrestrial plant C-N-P stoichiometry to an elevated global atmospheric CO in the future.
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http://dx.doi.org/10.1016/j.scitotenv.2018.09.051DOI Listing
February 2019

Chiral Landau levels in Weyl semimetal NbAs with multiple topological carriers.

Nat Commun 2018 05 10;9(1):1854. Epub 2018 May 10.

State Key Laboratory of Surface Physics and Department of Physics, Fudan University, 200433, Shanghai, China.

Recently, Weyl semimetals have been experimentally discovered in both inversion-symmetry-breaking and time-reversal-symmetry-breaking crystals. The non-trivial topology in Weyl semimetals can manifest itself with exotic phenomena, which have been extensively investigated by photoemission and transport measurements. Despite the numerous experimental efforts on Fermi arcs and chiral anomaly, the existence of unconventional zeroth Landau levels, as a unique hallmark of Weyl fermions, which is highly related to chiral anomaly, remains elusive owing to the stringent experimental requirements. Here, we report the magneto-optical study of Landau quantization in Weyl semimetal NbAs. High magnetic fields drive the system toward the quantum limit, which leads to the observation of zeroth chiral Landau levels in two inequivalent Weyl nodes. As compared to other Landau levels, the zeroth chiral Landau level exhibits a distinct linear dispersion in magnetic field direction and allows the optical transitions without the limitation of zero z momentum or [Formula: see text] magnetic field evolution. The magnetic field dependence of the zeroth Landau levels further verifies the predicted particle-hole asymmetry of the Weyl cones. Meanwhile, the optical transitions from the normal Landau levels exhibit the coexistence of multiple carriers including an unexpected massive Dirac fermion, pointing to a more complex topological nature in inversion-symmetry-breaking Weyl semimetals. Our results provide insights into the Landau quantization of Weyl fermions and demonstrate an effective tool for studying complex topological systems.
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http://dx.doi.org/10.1038/s41467-018-04080-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5945645PMC
May 2018

An Atypical Late Embryogenesis Abundant Protein OsLEA5 Plays a Positive Role in ABA-Induced Antioxidant Defense in Oryza sativa L.

Plant Cell Physiol 2018 May;59(5):916-929

College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.

OsLEA5 acts as a co-regulator of a transcriptional fact ZFP36 to enhance the expression and the activity of ascorbate peroxidase OsAPX1 to regulate seed germination in rice, but it it unknown whether OsLEA5 is also crucial in plant seedlings under stress conditions. To determine this, we generated OsLEA5 overexpression and knockdown rice plants. We found that overexpression of OsLEA5 in rice plants enhanced the tolerance to drought and salt stress; in contrast, an RNA interference (RNAi) mutant of OsLEA5 rice plants was more sensitive to drought and salinity. Further investigation found that various stimuli and ABA could induce OsLEA5 expression, and OsLEA5 acted downstream of ZFP36 to be involved in ABA-induced generation of hydrogen peroxide (H2O2), and the regulation of the expression and the activities of antioxidant defense enzymes in plants leaves, and OsLEA5 contributed to stabilize ZFP36. Additionally, OsLEA5 participates in the accumulation of ABA by up-regulating ABA biosynthesis genes and down-regulating ABA metabolism genes. Moreover, we found that two homologs of OsLEA5 (5C700, short for Os05g0526700; and 5C300, short for Os05g0584300) which were induced by ABA also interacted with ZFP36 separately; interestingly, the nuclear-located 5C700 could also act as a co-activator of ZFP36 to modulate OsAPX1, while 5C300 which was down-regulated by ABA induction acted as an ABA-induced inhibitor of ZFP36 to regulate OsAPX1. Hence, our conclusion is that OsLEA5 participates in the ABA-mediated antioxidant defense to function in drought and salt stress response in rice, and the 5C subgroup of LEAs contribute by acting as co-regulators of the transcription factor ZFP36.
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http://dx.doi.org/10.1093/pcp/pcy035DOI Listing
May 2018

Gas signaling molecule hydrogen sulfide attenuates doxorubicin-induced dilated cardiomyopathy.

Oncotarget 2017 Nov 8;8(56):95425-95431. Epub 2017 Sep 8.

Department of Cardiology, The Second Affiliated Hospital of Soochow University, Suzhou, China.

Increasing evidence has revealed that hydrogen sulfide (HS) has beneficial effects in the treatment of various cardiovascular diseases. However, whether HS can attenuate the development of dilated cardiomyopathy (DCM) remains unclear. In this study, we generated a rat model of DCM induced by doxorubicin and investigated the protective effects of HS against DCM. Cardiac structure and function were analyzed by two-dimensional echocardiography. Oxidative stress was evaluated by measuring malondialdehyde, superoxide dismutase, glutathione peroxidase and reactive oxygen species. Cardiomyocyte apoptosis was assessed by flow cytometry following Annexin V/PI staining. Our results showed that exogenous administration of HS could improve left ventricular structure and function in DCM rats. HS was found to suppress doxorubicin-induced oxidative stress by activating the Nrf2 pathway and upregulating the expression of antioxidant proteins NQO1 and GCLM. Moreover, HS was also found to inhibit doxorubicin-induced cardiomyocyte apoptosis by activating the PI3K/Akt signaling pathway. In conclusion, our study demonstrates that HS protects against doxorubicin-induced DCM via attenuation of oxidative stress and apoptosis.
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http://dx.doi.org/10.18632/oncotarget.20729DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5707032PMC
November 2017