Publications by authors named "Mengyao Wang"

91 Publications

IL-17A secreted by Th17 cells is essential for the host against infections.

J Microbiol Biotechnol 2021 Apr 21. Epub 2021 Apr 21.

College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, P.R. China.

is an important bacterial pathogen that is the causative agent of diseases, including neonatal sepsis and meningitis, and infections in pregnant women and non-pregnant adults. Although antibiotic treatments effectively relieve symptoms, the emergence and transmission of multidrug-resistant strains indicate the need for an effective immunotherapy. Effector T helper (Th) 17 cells is a relatively newly discovered subpopulation of helper CD4 T lymphocytes, by expressing interleukin (IL)-17A play crucial roles in host defenses against a variety of pathogens, including bacteria and viruses. However, whether infection can induce the differentiation of CD4 T cells into Th17 cells, and whether IL-17A can play an effective role against infections, are still unclear. In this study, we analyzed the responses of CD4 T cells and their defensive effects after infection. The results showed that infection induce not only the formation of Th1 cells expressing interferon (IFN)-γ, but also the differentiation of mouse splenic CD4 T cells into Th17 cells, which highly express IL-17A. In addition, the bacterial load of was significantly increased and decreased in organs as determined by antibody neutralization and IL-17A addition experiments, respectively. The results confirmed that IL-17A is required by the host to defend against and that it plays an important role in effectively eliminating . The results prompt us to adopt effective methods to regulate the expression of IL-17A as an effective strategy for the prevention from and treatment of infection.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.4014/jmb.2103.03053DOI Listing
April 2021

Long non-coding RNA GAS5 regulates Th17/Treg imbalance in childhood pneumonia by targeting miR-217/STAT5.

Cell Immunol 2021 Jun 1;364:104357. Epub 2021 Apr 1.

Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, China. Electronic address:

The imbalance of helper T (Th) 17 and regulatory T (Treg) cells plays an important role in the pathogenesis of pneumonia. This study aims to investigate the role and mechanism of long non-coding RNA growth arrest-specific 5 (GAS5) in the differentiation of Th17 cells and Tregs in childhood pneumonia. Expression of GAS5, miR-217, signal transducer and activator of transcription-5 (STAT5), receptor-related orphan receptor γt (RORγt), and transcription factor Forkhead box P3 (Foxp3) were examined by qRT-PCR and western blot. The percentage of Th17 cells and Tregs in CD4 T cells were measured by flow cytometry. The interaction between miR-217 and GAS5 or STAT5 was analyzed by luciferase reporter assay. Downregulated GAS5 expression and Treg cell percentage, and upregulated Th17 cell percentage were observed in pneumonia patients when compared with the healthy controls. Furthermore, GAS5 overexpression corrected the imbalanced Th17/Treg in peripheral blood CD4 T cells derived from pneumonia patients, and this effect was reversed by miR-217 mimic and STAT5 silencing. Mechanistically, GAS5 acted as a sponge of miR-217 to reduce binding of miR-217 to its target STAT5, leading to upregulation of STAT5 expression. Taken together, GAS5 corrects the Treg/Th17 imbalance by targeting the miR-217/STAT5 axis in childhood pneumonia.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cellimm.2021.104357DOI Listing
June 2021

Long non-coding RNA GAS5 regulates Th17/Treg imbalance in childhood pneumonia by targeting miR-217/STAT5.

Cell Immunol 2021 Jun 1;364:104357. Epub 2021 Apr 1.

Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, China. Electronic address:

The imbalance of helper T (Th) 17 and regulatory T (Treg) cells plays an important role in the pathogenesis of pneumonia. This study aims to investigate the role and mechanism of long non-coding RNA growth arrest-specific 5 (GAS5) in the differentiation of Th17 cells and Tregs in childhood pneumonia. Expression of GAS5, miR-217, signal transducer and activator of transcription-5 (STAT5), receptor-related orphan receptor γt (RORγt), and transcription factor Forkhead box P3 (Foxp3) were examined by qRT-PCR and western blot. The percentage of Th17 cells and Tregs in CD4 T cells were measured by flow cytometry. The interaction between miR-217 and GAS5 or STAT5 was analyzed by luciferase reporter assay. Downregulated GAS5 expression and Treg cell percentage, and upregulated Th17 cell percentage were observed in pneumonia patients when compared with the healthy controls. Furthermore, GAS5 overexpression corrected the imbalanced Th17/Treg in peripheral blood CD4 T cells derived from pneumonia patients, and this effect was reversed by miR-217 mimic and STAT5 silencing. Mechanistically, GAS5 acted as a sponge of miR-217 to reduce binding of miR-217 to its target STAT5, leading to upregulation of STAT5 expression. Taken together, GAS5 corrects the Treg/Th17 imbalance by targeting the miR-217/STAT5 axis in childhood pneumonia.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cellimm.2021.104357DOI Listing
June 2021

Growth and nitrate reductase activity are impaired in rice osnlp4 mutantssupplied with nitrate.

Plant Cell Physiol 2021 Mar 6. Epub 2021 Mar 6.

The Laboratory of Plant Nutrition and Fertilizers, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, 1138657, Japan.

Nitrate is an important nutrient and signaling molecule in plants, which modulates the expression of many genes and regulates plant growth. In paddy grown rice (Oryza sativa), nitrogen is mostly supplied in the form of ammonium, but can also be supplied in the form of nitrate. Several nitrogen transporters and nitrate assimilation enzymes have been identified and functionally characterized in rice. However, little is known regarding the nitrate sensing system in rice, and the regulatory mechanisms of nitrate-related genes remain to be elucidated. In recent years, NIN-like proteins (NLPs) have been described as key transcription factors of nitrogen responses in Arabidopsis thaliana, which implies that OsNLP4 is involved in the regulation of nitrate assimilation and nitrogen use efficiency in rice. Here, we show that OsNLP4 can influence plant growth by affecting nitrate reductase (NR) activity. The growth of OsNLP4 knockdown mutants was reduced when nitrate was supply, but not when ammonium was supply. The nitrate concentration was significantly reduced in osnlp4 mutants. Furthermore, the concentrations of iron and molybdenum, essential elements for NR activity, were reduced in OsNLP4 knockdown mutants. We propose that, in addition to the regulation of gene expression within the nitrate signaling pathway, OsNLP4 can affect the NR activity and nitrate-dependent growth of rice. Our results support a working model for the role of OsNLP4 in the nitrate signaling pathway.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/pcp/pcab035DOI Listing
March 2021

Rehabilitation training based on virtual reality for patients with Parkinson's disease in improving balance, quality of life, activities of daily living, and depressive symptoms: A systematic review and meta-regression analysis.

Clin Rehabil 2021 Feb 15:269215521995179. Epub 2021 Feb 15.

Alice Lee Centre for Nursing Studies, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.

Objective: To examine the effectiveness of rehabilitation training based on virtual reality in improving balance, quality of life, activities of daily living, and depressive symptoms of patients with Parkinson's disease.

Data Sources: PubMed, EMBASE, CINAHL, Scopus, Cochrane Library, PsycINFO, ProQuest, Physiotherapy Evidence Database, IEEE Xplore, China National Knowledge Infrastructure, Wanfang, and VIP Information databases were searched from their inception to October 15, 2020. Trial registries, gray literature, and target journals were also searched.

Methods: Eligible randomized controlled trials included studies with patients with Parkinson's disease in rehabilitation training based on virtual reality. Comprehensive Meta-Analysis 3.0 software was used. Physiotherapy Evidence Database Scale and the Grading of Recommendation, Assessment, Development, and Evaluation system were used to assess the methodological quality of individual trials and the overall quality of the evidence, respectively.

Results: A total of 22 randomized controlled trials with 836 patients were included. Meta-analysis revealed that training significantly improved balance ( = 0.66,  < 0.001), quality of life ( = 0.28,  = 0.015), activities of daily living ( = 0.62,  < 0.001), and depressive symptoms ( = 0.67,  = 0.021) compared to the control group. Subgroup analysis indicated that training should utilize video game consoles. Meta-regression analyses showed that age, sessions, and frequency of training had statistically significant impacts on balance scores. Quality of individual trials was high and overall evidence ranged from very low to low.

Conclusion: Virtual rehabilitation training could be adopted in healthcare institutions as supplementary training for patients with Parkinson's disease.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/0269215521995179DOI Listing
February 2021

[Research progress on quality of life in patients with chronic rhino sinusitis].

Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2021 01;35(1):84-87

With the development of modern medical technology, people not only require the extention of life, but also pay more attention to the quality of life. Chronic rhino-sinusitis is a common disease in Otolaryngology Head and Neck Surgery, and its incidence has increased slightly in recent years. The symptoms seriously disturb patients' daily life and affected patients' quality of life. This review first describes the current status of quality of life for patients with chronic sinusitis, secondly analyzes the factors that affect the quality of life of patients with chronic sinusitis, describes various quality of life scales for chronic rhinosinusitis, and finally conducts a comprehensive evaluation of future research directions.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.13201/j.issn.2096-7993.2021.01.022DOI Listing
January 2021

Underwater, Multifunctional Superhydrophobic Sensor for Human Motion Detection.

ACS Appl Mater Interfaces 2021 Jan 28;13(3):4740-4749. Epub 2020 Dec 28.

College of Chemical Engineering, Fuzhou University, Fuzhou 350116, P. R. China.

Superhydrophobic conductive materials have received a great amount of interest due to their wide applications in oil-water separation, electrically driven smart surface, electromagnetic shielding, and body motion detection. Herein, a highly conductive superhydrophobic cotton cloth is prepared by a facile method. A layer of polydopamine/reduced graphene oxide (PDA/rGO) was first coated on the cotton fabric, and then copper nanoparticles were in situ grown on the prepared surface. After further modification with stearic acid (STA), the wettability of the cotton surface changed from superhydrophilic to superhydrophobic (water contact angle (WCA) = 153°). The electrical conductivity of the PDA/rGO/Cu/STA cotton is as high as 6769 S·m, while the stearic acid effectively protects Cu NPs from oxidation. As a result, the superhydrophobic PDA/rGO/Cu/STA cotton has shown excellent electrical stability and can be used in detecting human motions in both ambient and underwater conditions. The sensor can recognize human motion from air into water and other underwater activities (e.g., underwater bending, stretching, and ultrasound). This multifunctional cotton device can be used as an ideal sensor for underwater intelligent devices and provides a basis for further research.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.0c19704DOI Listing
January 2021

Mica Can Alleviate TNBS-Induced Colitis in Mice by Reducing Angiotensin II and IL-17A and Increasing Angiotensin-Converting Enzyme 2, Angiotensin 1-7, and IL-10.

Mediators Inflamm 2020 10;2020:3070345. Epub 2020 Oct 10.

Department of Gastroenterology, First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou City, Zhejiang Province 310006, China.

Aim: To explore the treatment effect of mica on 2,4,6-trinitrobenzenesulfonic acid solution- (TNBS-) induced colitis in mice.

Materials And Methods: Thirty male BALB/C mice were randomly divided into the control group, the TNBS group, and the mica group. Control mice were treated with saline solution. Experimental colitis was induced by TNBS (250 mg/kg/d) in the TNBS group and the mica group. After modeling, the mica group was treated with mica (180 mg/kg/d) for 3 days, while the TNBS group continued the treatment with TNBS. All solutions were injected intrarectally. During treatment, body weight and mice activity were monitored daily. After treatment, the colon tissues of mice were collected; angiotensin II (Ang II), angiotensin-converting enzyme 2 (ACE2), angiotensin 1-7 (Ang (1-7)), IL-17A, and IL-10 expression was analyzed by ELISA and immunohistochemistry.

Results: Food intake, activity, and body weight gradually decreased in the TNBS group compared to the control group and the mica group (all < 0.05). Also, black stool adhesion in the anus and thin and bloody stool were observed in the TNBS group, but not in the other two groups. Moreover, the expression of Ang II, ACE2, Ang (1-7), IL-17A, and IL-10 in the TNBS group increased compared to that in the control group. Compared to the TNBS group, ACE2, Ang (1-7), and IL-10 in the mica group increased, while Ang II and IL-17A decreased (all < 0.05).

Conclusion: Mica can alleviate TNBS-induced colitis in mice by regulating the inflammation process; it reduces Ang II and IL-17A and increases ACE2, IL-10, and Ang (1-7).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2020/3070345DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7569463PMC
October 2020

14,15-Epoxyeicosatrienoic Acid Alleviates Pathology in a Mouse Model of Alzheimer's Disease.

J Neurosci 2020 10 24;40(42):8188-8203. Epub 2020 Sep 24.

Institute of Mental Health, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, People's Republic of China

Alzheimer's disease (AD) is the leading cause of late-onset dementia, and there exists an unmet medical need for effective treatments for AD. The accumulation of neurotoxic amyloid-β (Aβ) plaques contributes to the pathophysiology of AD. encoding soluble epoxide hydrolase (sEH)-a key enzyme for epoxyeicosatrienoic acid (EET) signaling that is mainly expressed in lysosomes of astrocytes in the adult brain-is cosited at a locus associated with AD, but it is unclear whether and how it contributes to the pathophysiology of AD. In this report, we show that the pharmacologic inhibition of sEH with 1-trifluoromethoxyphenyl- 3-(1-propionylpiperidin-4-yl) urea (TPPU) or the genetic deletion of reduces Aβ deposition in the brains of both male and female familial Alzheimer's disease (5×FAD) model mice. The inhibition of sEH with TPPU or the genetic deletion of alleviated cognitive deficits and prevented astrocyte reactivation in the brains of 6-month-old male 5×FAD mice. 14,15-EET levels in the brains of these mice were also increased by sEH inhibition. In cultured adult astrocytes treated with TPPU or 14,15-EET, astrocyte Aβ clearance was increased through enhanced lysosomal biogenesis. Infusion of 14,15-EET into the hippocampus of 5×FAD mice prevented the aggregation of Aβ. Notably, a higher concentration of 14,15-EET (200 ng/ml) infusion into the hippocampus reversed Aβ deposition in the brains of 6-month-old male 5×FAD mice. These results indicate that EET signaling, especially 14,15-EET, plays a key role in the pathophysiology of AD, and that targeting this pathway is a potential therapeutic strategy for the treatment of AD. There are limited treatment options for Alzheimer's disease (AD). encoding soluble epoxide hydrolase (sEH) is located at a locus that is linked to late-onset AD, but its contribution to the pathophysiology of AD is unclear. Here, we demonstrate that sEH inhibition or deletion alleviates pathology in familial Alzheimer's disease (5×FAD) mice. Inhibiting sEH or increasing 14,15-epoxyeicosatrienoic acid (EET) enhanced lysosomal biogenesis and amyloid-β (Aβ) clearance in cultured adult astrocytes. Moreover, the infusion of 14,15-EET into the hippocampus of 5×FAD mice not only prevented the aggregation of Aβ, but also reversed the deposition of Aβ. Thus, 14,15-EET plays a key role in the pathophysiology of AD and therapeutic strategies that target this pathway may be an effective treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1523/JNEUROSCI.1246-20.2020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7574654PMC
October 2020

Human activities and elevational constraints restrict ranging patterns of snub-nosed monkeys in a mountainous refuge.

Integr Zool 2021 Mar 10;16(2):202-213. Epub 2020 Oct 10.

Shaanxi Key Laboratory for Animal Conservation, College of Life Sciences, Northwest University, Xi'an, China.

Both natural conditions and anthropogenic factors affect the survivability, distribution, and population density of wildlife. To understand the extent and how these factors drive species distributions, a detailed description of animal movement patterns in natural habitats is needed. In this study, we used satellite telemetry to monitor elevational ranges favored by endangered golden snub-nosed monkeys (Rhinopithecus roxellana), in the Qinling Mountains, central China. We investigated the abundance and distribution of food resources through sampling vegetation quadrats at different elevations and sampled anthropogenic activities using field surveys. Our results indicated that although there was no significant variation in food resources between low- (<1500 m) and middle-elevations (1500-2200 m), monkeys were found most often in areas above 1500 m, where there was less anthropogenic development (e.g. houses and roads); however, monkeys rarely ranged above 2200 m and had limited food availability at this altitude. There was limited human disturbance at this elevation. We suggest that both human activity and ecological constraints (i.e. food resources) have considerable effects on elevational use of R. roxellana in the Qinling Mountains. This study highlights the critical roles these factors can play in shaping the vertical distribution of high-altitude primates. This research provides useful insights for habitat-based conservation plans in which human disturbance management and habitat restoration should be prioritized.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/1749-4877.12490DOI Listing
March 2021

is transcriptionally regulated by E2F3, and its depletion leads to mitotic catastrophe in esophageal squamous cell carcinoma.

Ann Transl Med 2020 Aug;8(15):950

Department of Medical Oncology, the Affiliated Tumor Hospital of Zhengzhou University, Henan Cancer Hospital, Zhengzhou, China.

Background: RACGAP1 has significant involvement in tumorigenesis of cancers, including liver cancer, stomach cancer, and colon cancer. However, the role and the exact mechanism of RACGAP1 in esophageal squamous cell carcinoma (ESCC) has not been explored.

Methods: QPCR and Western blots analysis was performed to analyze the expression of RACGAP1 in ESCC. MTT assays and colony formation assays were performed to explore the functional role of RACGAP1 in ESCC. Cell cycle analysis and immunofluorescence assays were used to investigate the function of RACGAP1 involvement in mitotic catastrophe. At last, we conducted the public datasets mining to explore the expression status and prognosis value of RACGAP1 as well as the correlation between RACGAP1 and E2F3 in various cancers.

Results: The high abnormal expression of RACGAP1 is observed in ESCC and associated with worse clinical outcomes of patients with ESCC. , a novel cell cycle associated gene regulated by E2F3, acts as an oncogenic driver in ESCC cell lines. Notably, for the first time, RACGAP1 depletion induced severe mitotic catastrophe, followed by massive cell death.

Conclusions: Our findings showed the essential role of RACGAP1 in ESCC cancer cell survival and the therapeutic potential of RACGAP1 as a molecular target for ESCC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.21037/atm-20-2901DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7475413PMC
August 2020

Impaired perceptual processing of facial expression categorization in social anhedonia.

Psychophysiology 2020 12 17;57(12):e13682. Epub 2020 Sep 17.

Department of Psychology, Dalian Medical University, Dalian, China.

The current event-related potential study investigated the perceptual processing of the categorization advantage of happy over sad faces in social anhedonia with a nonclinical sample. A high social anhedonia (HSA, N = 25) group and a low social anhedonia (LSA, N = 27) group performed a facial expression categorization task during which they classified facial expressions (happy, neutral, sad), irrespective of face orientation (upright, upside-down). Behaviorally, happy faces were identified more quickly than sad ones in the upright but not inverted orientation. Electrophysiologically, the LSA group showed earlier N170 latencies for happy than for sad faces in the upright but not upside-down orientation, whereas the HSA group did not show any expression effect on N170 latencies. Moreover, N170 and P2 amplitude results revealed that HSA relative to LSA individuals showed delayed neural discrimination between happy and sad faces. These results suggest that social anhedonia is associated with a deficit of perceptual processing during facial expression categorization.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/psyp.13682DOI Listing
December 2020

Genetic Heterogeneity Between Paired Primary and Brain Metastases in Lung Adenocarcinoma.

Clin Med Insights Oncol 2020 25;14:1179554920947335. Epub 2020 Aug 25.

Department of Respiratory Disease, Daping Hospital, Army Medical University, Chongqing, P.R. China.

Purpose: About one-third of nonsmall cell lung cancer (NSCLC) patients develop brain metastases (BM). However, there is an unmet need for early diagnosis and treatment of BM. The precise mechanism for BM is still unknown. However, the genetic heterogeneity between primary tumor and paired BM indicates that sampling from the primary tumor may not be able to fully represent the mutational status in metastases. In this study, the genetic heterogeneity of primary lung adenocarcinoma and paired BM was analyzed.

Patients And Methods: A total of 11 paired samples of primary tumors and BM from lung cancer patients were included, in which 7 paired samples of patients were finally analyzed. Samples were sequenced by whole-exome sequencing (WES) to investigate the common and unique mutations in the primary tumors and BM, and the similarities and differences in copy number variation (CNV).

Results: The consistency of gene mutation between primary lung adenocarcinoma and paired BM was 33% to 86%. FAM129C and ADAMTSs specifically mutated in BM, along with NKX2-1 high amplification and SAMD2/4 copy number deletion.

Conclusion: The consistency of gene mutation between primary lung adenocarcinoma and corresponding BM is relatively high, while the individual differences were significant. FAM129C and ADAMTSs mutations and high amplification of NKX2-1 may be related to BM of lung cancer. The loss of copy number of SAMD2/4 may be a potential therapeutic target for BM from lung adenocarcinoma.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/1179554920947335DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7450461PMC
August 2020

Studying CNS effects of Traditional Chinese Medicine using zebrafish models.

J Ethnopharmacol 2021 Mar 9;267:113383. Epub 2020 Sep 9.

School of Pharmacy, Southwest University, Chongqing, China; Ural Federal University, Ekaterinburg, Russia. Electronic address:

Ethnopharmacological Relevance: Although Traditional Chinese Medicine (TCM) has a millennia-long history of treating human brain disorders, its complex multi-target mechanisms of action remain poorly understood. Animal models are currently widely used to probe the effects of various TCMs on brain and behavior. The zebrafish (Danio rerio) has recently emerged as a novel vertebrate model organism for neuroscience research, and is increasingly applied for CNS drug screening and development.

Aim Of The Study: As zebrafish models are only beginning to be applied to studying TCM, we aim to provide a comprehensive review of the TCM effects on brain and behavior in this fish model species.

Materials And Methods: A comprehensive search of published literature was conducted using biomedical databases (Web of Science, Pubmed, Sciencedirect, Google Scholar and China National Knowledge Internet, CNKI), with key search words zebrafish, brain, Traditional Chinese Medicine, herbs, CNS, behavior.

Results: We recognize the developing utility of zebrafish for studying TCM, as well as outline the existing model limitations, problems and challenges, as well as future directions of research in this field.

Conclusions: We demonstrate the growing value of zebrafish models for studying TCM, aiming to improve our understanding of TCM' therapeutic mechanisms and potential in treating brain disorders.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jep.2020.113383DOI Listing
March 2021

Morphological and physiological factors contributing to early vigor in the elite rice cultivar 9,311.

Sci Rep 2020 09 9;10(1):14813. Epub 2020 Sep 9.

National Key Laboratory for Plant Molecular Genetics, Chinese Academy of Science (CAS) Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, CAS, Building No. 1, Room 402, Fenglin Road 300, Shanghai, 200032, China.

Huanghuazhan (HHZ) and 9,311 are two elite rice cultivars in China. They have achieved high yield through quite different mechanisms. One of the major features that gives high yield capacity to 9,311 is its strong early vigor, i.e., faster establishment of its seedling as well as its better growth in its early stages. To understand the mechanistic basis of early vigor in 9,311, as compared to HHZ the cultivar, we have examined, under controlled environmental conditions, different morphological and physiological traits that may contribute to its early vigor. Our results show that the fresh weight of the seeds, at germination, not only determined the seedling biomass at 10 days after germination (DAG), but was also responsible for ~ 80% of variations in plant biomass between the two cultivars even up to 30 DAG. Furthermore, the 9,311 cultivar had a larger root system, which led to its higher nitrogen uptake capacity. Other noteworthy observations about 9,311 being a better cultivar than HHZ are: (i) Ten out of 15 genes involved in nitrogen metabolism were much more highly expressed in its roots; (ii) it had a higher water uptake rate, promoting better root-to-shoot nitrogen transfer; and (iii) consistent with the above, it had higher leaf photosynthetic rate and stomatal conductance. All of the above identified features explain, to a large extent, why the 9,311, as compared to HHZ, exhibits much more vigorous early growth.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-020-71913-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7481250PMC
September 2020

Active pharmaceutical ingredient poly(ionic liquid)-based microneedles for the treatment of skin acne infection.

Acta Biomater 2020 10 25;115:136-147. Epub 2020 Aug 25.

Jiangsu Key Laboratory of Advanced Functional Polymer Design and Application, Department of Polymer Science and Engineering, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, China.. Electronic address:

As an inflammatory skin disease of pilosebaceous follicles, Propionibacterium acnes (P. acnes) can aggravate local inflammatory responses and forms acne lesions. However, due to the skin barrier, various transdermal measures other than antibiotic creams are necessary. Microneedle (MN) patches are emerging platforms for the transdermal delivery of various therapeutics since it can effectively create transport pathways in the epidermis. Herein, we develop an active pharmaceutical ingredient poly(ionic liquid) (API PIL)-based MN patches containing salicylic acid (SA). The PIL-based MNs are simply prepared through photo-crosslinking of an imidazolium-type ionic liquid (IL) monomer in MN micro-molds, and following by anion exchange with salicylic acid anions (SA). The fabricated SA-loaded PIL-MNs exhibited therapeutic efficiency in the topical treatment of P. acnes infection in vitro and in vivo. These active pharmaceutical ingredient PIL-based MNs can improve acne treatment, demonstrating potential applications for skin diseases. STATEMENT OF SIGNIFICANCE: Microneedle (MN) patches can be used as platforms for transdermal delivery of various therapeutics to treat bacterial infection. Here, a facile strategy was developed to synthesize active pharmaceutical ingredient poly(ionic liquid)-based microneedle patches by anion-exchange with salicylic acid anion (SA). The fabricated SA-loaded PIL-MNs are active on not only anti-bacteria but also anti-inflammation in P. acnes treated mice, and may have potential applications for skin acne infection.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.actbio.2020.08.023DOI Listing
October 2020

A dielectric metasurface optical chip for the generation of cold atoms.

Sci Adv 2020 Jul 29;6(31):eabb6667. Epub 2020 Jul 29.

Department of Materials Science and Engineering, Southern University of Science and Technology, Shenzhen 518055, China.

Compact and robust cold atom sources are increasingly important for quantum research, especially for transferring cutting-edge quantum science into practical applications. In this study, we report on a novel scheme that uses a metasurface optical chip to replace the conventional bulky optical elements used to produce a cold atomic ensemble with a single incident laser beam, which is split by the metasurface into multiple beams of the desired polarization states. Atom numbers ~10 and temperatures (about 35 μK) of relevance to quantum sensing are achieved in a compact and robust fashion. Our work highlights the substantial progress toward fully integrated cold atom quantum devices by exploiting metasurface optical chips, which may have great potential in quantum sensing, quantum computing, and other areas.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1126/sciadv.abb6667DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7439576PMC
July 2020

Ammonia exposure causes lung injuries and disturbs pulmonary circadian clock gene network in a pig study.

Ecotoxicol Environ Saf 2020 Dec 19;205:111050. Epub 2020 Aug 19.

Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, 430070, China. Electronic address:

Ammonia toxicity to respiratory system in pig faming is of particular concern, but the molecular mechanism remains still unclear. The present study was devoted to assess the impacts of the ammonia exposure on the lung tissues based on a pig study using 80 ppm ammonia exposing to piglets for different days. The histology analysis revealed ammonia exposure induced lung injury and inflammatory response, as indicated by epithelial-mesenchymal transition (EMT), significant thickening of alveolar septa, infiltration of inflammatory cells and excessive mucus production. The transcriptome analysis revealed many more up-regulated genes in exposure groups when compared with the control group, and these genes were significantly enriched in the GO term of extracellular exosome, proteolysis, and regulation of circadian rhythm. The study discovered the induction of seven genes (CRY2, CIART, CREM, NR1D1, NR1D2, PER1 and PER3) that encode repressors of circadian clock. One gene (ARNTL) that encodes activator of circadian clock was down-regulated after ammonia exposure. The results of this study suggest that ammonia exposure disturbed the pulmonary circadian clock gene expression, which may establish new evidence for further understanding the toxicity of ammonia to lungs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ecoenv.2020.111050DOI Listing
December 2020

Functional dissection of HGGT and HPT in barley vitamin E biosynthesis via CRISPR/Cas9-enabled genome editing.

Ann Bot 2020 10;126(5):929-942

Institute of Genetics and Regenerative Biology, Key Laboratory for Cell and Gene Engineering of Zhejiang Province, College of Life Sciences, Zhejiang University, Hangzhou, China.

Background And Aims: Vitamin E (tocochromanol) is a lipid-soluble antioxidant and an essential nutrient for human health. Among cereal crops, barley (Hordeum vulgare) contains a high level of vitamin E, which includes both tocopherols and tocotrienols. Although the vitamin E biosynthetic pathway has been characterized in dicots, such as Arabidopsis, which only accumulate tocopherols, knowledge regarding vitamin E biosynthesis in monocots is limited because of the lack of functional mutants. This study aimed to obtain gene knockout mutants to elucidate the genetic control of vitamin E composition in barley.

Methods: Targeted knockout mutations of HvHPT and HvHGGT in barley were created with CRISPR/Cas9-enabled genome editing. High-performance liquid chromatography (HPLC) was performed to analyse the content of tocochromanol isomers in transgene-free homozygous Hvhpt and Hvhggt mutants.

Key Results: Mutagenesis efficiency among T0 regenerated plantlets was 50-65 % as a result of two simultaneously expressed guide RNAs targeting each gene; most of the mutations were stably inherited by the next generation. The transgene-free homozygous mutants of Hvhpt and Hvhggt exhibited decreased grain size and weight, and the HvHGGT mutation led to a shrunken phenotype and significantly lower total starch content in grains. HPLC analysis revealed that targeted mutation of HvHPT significantly reduced the content of both tocopherols and tocotrienols, whereas mutations in HvHGGT completely blocked tocotrienol biosynthesis in barley grains. Transient overexpression of an HvHPT homologue in tobacco leaves significantly increased the production of γ- and δ-tocopherols, which may partly explain why targeted mutation of HvHPT in barley grains did not eliminate tocopherol production.

Conclusions: Our results functionally validated that HvHGGT is the only committed gene for the production of tocotrienols, whereas HvHPT is partly responsible for tocopherol biosynthesis in barley.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/aob/mcaa115DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7539355PMC
October 2020

Ammonia Exposure Induced Cilia Dysfunction of Nasal Mucosa in the Piglets.

Biomed Res Int 2020 25;2020:1705387. Epub 2020 May 25.

Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.

As one of the main environmental stressors commonly found in closed pig houses, ammonia poses high risks to the well-being of humans and animals. This study is aimed at assessing the toxicity of ammonia exposure (80 ppm for 12 days) on the nasal mucosa in piglets. Firstly, we found that after ammonia exposure, the number of white blood cells significantly increased and the serum levels of cytokine IL-4 were significantly decreased. Then, histological analyses showed significant thickening of nasal mucosa and excessive mucus production in the exposure group. Finally, RNA-seq analyses demonstrated that the ammonia exposure disturbed the transcriptome of nasal mucosa which revealed 176 upregulated genes and 426 downregulated genes. GO and KEGG pathway enrichment analysis of the DEGs showed that the upregulated genes were mainly related to neutrophil chemotaxis and immune response, while 80 out of the 426 downregulated genes including CCDCs, CFAPs, DNAHs, and TEKTs were enriched in the microtubule cytoskeleton and cilium morphogenesis/movement. All these results indicated that ammonia exposure induces nasal mucosal hyperplasia and cilia dysfunction, as well as a systemic inflammatory response in piglets. These findings provide new evidence for understanding the damage mechanism of ammonia on the nasal mucosa.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2020/1705387DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7273420PMC
March 2021

Farnesoid X receptor mediates hepatic steatosis induced by PM.

Environ Sci Pollut Res Int 2020 Sep 17;27(27):34412-34420. Epub 2020 Jun 17.

Center for Clinical Pharmacology, The Third Xiangya Hospital, Central South University, Changsha, 410013, China.

Ambient particulate matter (PM) newly has been regarded as a conceivable hazard for public health. A large number of studies have described that PM, exceptionally PM, is correlated with respiratory, cardiovascular, and metabolic diseases, etc. PM-induced hepatocyte steatosis previously has been uncovered both in cellular and murine models. Nevertheless, less is known about the underlying mechanism. Here, we found that PM could cause the downregulation of farnesoid X receptor (FXR), a key transcription factor for lipid metabolism. FXR could regulate the accumulation of lipid droplets induced by PM in vitro. Moreover, FXR mice were exposed to PM for 2 months to investigate the role of FXR in pathogenesis of PM-induced hepatic steatosis in vivo. The results showed that exposure of wild-type (WT) mice to PM caused mild liver steatosis compared with the mice exposure to filtered air (FA). Furthermore, the content of triglyceride (TG) and total cholesterol (TC) was elevated in WT mice liver triggered by the inhalation of PM. However, there was no statistical difference in TG and TC content between FXR mice with and without PM exposure. Overall, our finding suggested FXR mediated PM-induced hepatic steatosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11356-020-09676-2DOI Listing
September 2020

[Mismatch of ASPECTS based on arterial spin labeling and diffusion-weighted imaging as an indicator for mechanical thrombectomy in patients with wake-up stroke].

Nan Fang Yi Ke Da Xue Xue Bao 2020 Jan;40(1):1-5

Department of Neurology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.

Objective: To retrospectively analyze the outcomes of wake-up stroke (WUS) patients with occlusion of large vessel occlusion (LVO), who were selected for mechanical thrombectomy according to the mismatch of Alberta Stroke Program Early CT Score (ASPECTS) based on arterial spin labeling (ASL) and diffusion-weighted image (DWI) on admission magnetic resonance (MR) scans.

Methods: Twelve consecutive WUS patients with acute LVO of the anterior circulation undergoing MR scans with ASL and DWI prior to thrombectomy were retrospectively evaluated. The mismatch of ASPECTS was defined as the difference between ASL-ASPECTS and DWI-ASPECTS, and a higher score indicates a greater mismatch.

Results: The procedures led to successful reperfusion in all the cases (Thrombolysis in Cerebral Infarction Grade 2b-3). Eleven patients (91.7%) had significantly decreased National Institute of Health Stroke scale (NIHSS) score at discharge.AmRS score of ≤2 at 90 days was achieved in 8 of the 12 patients (66.7%).

Conclusions: The mismatch between ASPECTS assessed based on ASL and DWI can detect a true mismatch in patients with acute LVO of the anterior circulation, and can be used for rapid screening of patients eligible for thrombectomy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.12122/j.issn.1673-4254.2020.01.01DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7040767PMC
January 2020

TRIM21 and PHLDA3 negatively regulate the crosstalk between the PI3K/AKT pathway and PPP metabolism.

Nat Commun 2020 04 20;11(1):1880. Epub 2020 Apr 20.

The MOE Key Laboratory of Cell Proliferation and Differentiation, School of Life Sciences, Peking-Tsinghua Center for Life Sciences, Beijing Advanced Innovation Center for Genomics, Peking University, Beijing, 100871, China.

PI3K/AKT signaling is known to regulate cancer metabolism, but whether metabolic feedback regulates the PI3K/AKT pathway is unclear. Here, we demonstrate the important reciprocal crosstalk between the PI3K/AKT signal and pentose phosphate pathway (PPP) branching metabolic pathways. PI3K/AKT activation stabilizes G6PD, the rate-limiting enzyme of the PPP, by inhibiting the newly identified E3 ligase TIRM21 and promotes the PPP. PPP metabolites, in turn, reinforce AKT activation and further promote cancer metabolic reprogramming by blocking the expression of the AKT inhibitor PHLDA3. Knockout of TRIM21 or PHLDA3 promotes crosstalk and cell proliferation. Importantly, PTEN null human cancer cells and in vivo murine models are sensitive to anti-PPP treatments, suggesting the importance of the PPP in maintaining AKT activation even in the presence of a constitutively activated PI3K pathway. Our study suggests that blockade of this reciprocal crosstalk mechanism may have a therapeutic benefit for cancers with PTEN loss or PI3K/AKT activation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-020-15819-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7170963PMC
April 2020

Virulence and Immune Escape.

Microorganisms 2020 Mar 13;8(3). Epub 2020 Mar 13.

Beijing Key Laboratory for Animal Genetic Improvement, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.

genus represents the most common foodborne pathogens causing morbidity, mortality, and burden of disease in all regions of the world. The introduction of antimicrobial agents and Salmonella-specific phages has been considered as an effective intervention strategy to reduce Salmonella contamination. However, data from the United States, European countries, and low- and middle-income countries indicate that Salmonella cases are still a commonly encountered cause of bacterial foodborne diseases globally. The control programs have not been successful and even led to the emergence of some multidrug-resistant Salmonella strains. It is known that the host immune system is able to effectively prevent microbial invasion and eliminate microorganisms. However, has evolved mechanisms of resisting host physical barriers and inhibiting subsequent activation of immune response through their virulence factors. There has been a high interest in understanding how interacts with the host. Therefore, in the present review, we characterize the functions of virulence genes and particularly focus on the mechanisms of immune escape in light of evidence from the emerging mainstream literature.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/microorganisms8030407DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7143636PMC
March 2020

Functional heterogeneity of perceived control in feedback processing.

Soc Cogn Affect Neurosci 2020 05;15(3):329-336

Department of Neurology and Psychiatry, First Affiliated Hospital, Dalian Medical University, Dalian 116011, China.

Perceived control is a fundamental psychological function that can either boost positive affect or buffer negative affect. The current study addressed the electrophysiological correlates underlying perceived control, as exercised by choice, in the processing of feedback valence. Thirty-six participants performed an EEG choice task during which they received positive or negative feedback following choices made either by themselves or by a computer. Perceived control resulted in an enhanced reward positivity for positive feedback but increased theta power for negative feedback. Further, perceived control led to greater feedback P3 amplitude and delta power, regardless of feedback valence. These results suggest functional heterogeneity of perceived control in feedback processing as diverse as magnifying the reward signal, enhancing the need for control and increasing the motivational salience of outcome irrespective of valence.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/scan/nsaa028DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7235962PMC
May 2020

Structure and physicochemical properties of two waxy wheat starches.

Food Chem 2020 Jul 25;318:126492. Epub 2020 Feb 25.

Department of Grain Science and Industry, Kansas State University, Manhattan, KS 66506, USA. Electronic address:

The objective of this study was to determine the primary influences of amylopectin (AP) on starch properties using two waxy wheat starches with different pasting properties. Compared with Yang Nuo Mai 1 (YNM1) starch, Ning Nuo Mai 1 (NNM1) starch had a greater average granule diameter, hot water solubility, gelatinization enthalpy, higher pasting viscosity, but lower retrogradation enthalpy. NNM1 starch had a greater proportion of AP long chains, and lower proportion of AP short chains. Therefore, NNM1 starch had a greater degree of crystallinity as well as stronger resonance intensities at the C1, C2, C3, C5, and C6 by solid-state C CP/MAS NMR. These results suggested that the proportion of long AP chains (DP ≥ 37) is the main influencing factor in determining waxy starch physicochemical properties. In addition, the proportion of short AP chains (DP 13-24) plays a role in promoting retrogradation of waxy wheat starch.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.foodchem.2020.126492DOI Listing
July 2020

Cross-species Analyses of Intra-species Behavioral Differences in Mammals and Fish.

Neuroscience 2020 03 10;429:33-45. Epub 2020 Jan 10.

School of Pharmacy, Southwest University, Chongqing, China; Ural Federal University, Ekaterinburg, Russia. Electronic address:

Multiple species display robust behavioral variance among individuals due to different genetic, genomic, epigenetic, neuroplasticity and environmental factors. Behavioral individuality has been extensively studied in various animal models, including rodents and other mammals. Fish, such as zebrafish (Danio rerio), have recently emerged as powerful aquatic model organisms with overt individual differences in behavioral, nociceptive and other CNS traits. Here, we evaluate individual behavioral differences in mammals and fish, emphasizing the importance of cross-species analyses of intraspecies variance in experimental models of normal and pathological CNS functions.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.neuroscience.2019.12.035DOI Listing
March 2020

DARK Classics in Chemical Neuroscience: Kava.

ACS Chem Neurosci 2020 Jan 21. Epub 2020 Jan 21.

School of Pharmacy, Southwest University, Chongqing 400700, China.

Kava (kava kava, ) is a common drug-containing plant in the Pacific islands. Kavalactones, its psychoactive compounds, exert potent central nervous system (CNS) action clinically and in animal models. However, the exact pharmacological profiles and mechanisms of action of kava on the brain and behavior remain poorly understood. Here, we discuss clinical and experimental data on kava psychopharmacology and summarize chemistry and synthesis of kavalactones. We also review its societal impact, drug use and abuse potential, and future perspectives on translational kava research.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acschemneuro.9b00587DOI Listing
January 2020