Publications by authors named "Mengyang Wang"

63 Publications

Neural Substrates of External and Internal Visual Sensations Induced by Human Intracranial Electrical Stimulation.

Front Neurosci 2022 22;16:918767. Epub 2022 Jul 22.

CAS Key Laboratory of Mental Health, Institute of Psychology, Beijing, China.

Offline perceptions are self-generated sensations that do not involve physical stimulus. These perceptions can be induced by external hallucinated objects or internal imagined objects. However, how the brain dissociates these visual sensations remains unclear. We aimed to map the brain areas involved in internal and external visual sensations induced by intracranial electrical stimulation and further investigate their neural differences. In this study, we collected subjective reports of internal and external visual sensations elicited by electrical stimulation in 40 drug-refractory epilepsy during presurgical evaluation. The response rate was calculated and compared to quantify the dissociated distribution of visual responses. We found that internal and external visual sensations could be elicited when different brain areas were stimulated, although there were more overlapping brain areas. Specifically, stimulation of the hippocampus and inferior temporal cortex primarily induces internal visual sensations. In contrast, stimulation of the occipital visual cortex mainly triggers external visual sensations. Furthermore, compared to that of the dorsal visual areas, the ventral visual areas show more overlap between the two visual sensations. Our findings show that internal and external visual sensations may rely on distinct neural representations of the visual pathway. This study indicated that implantation of electrodes in ventral visual areas should be considered during the evaluation of visual sensation aura epileptic seizures.
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http://dx.doi.org/10.3389/fnins.2022.918767DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355733PMC
July 2022

How to Precisely Open the Internal Auditory Canal for Resection of Vestibular Schwannoma the Retrosigmoid Approach.

Front Surg 2022 28;9:889402. Epub 2022 Jun 28.

Department of neurosurgery, Zhongnan Hospital, Wuhan University, Wuhan, China.

Objective: The aim of this study was to investigate how to precisely expose the intrameatal portion of vestibular schwannomas (VSs) without damaging the labyrinth.

Methods: This was a retrospective study of patients who had undergone retrosigmoid resection of a VS in our institution from April 2018 to December 2021. The patients were divided into microsurgery (MS) and navigation endoscopic-assisted (combined surgery, CS) groups and the effects of image guidance and endoscopy evaluated. The tumors in the CS group were then divided into medial and lateral types by fusion imaging and the differences between the two types analyzed.

Results: Data of 84 patients were analyzed. Residual tumor was detected by postoperative MRI at the fundus of the internal auditory canal in 5 of the 31 patients in the MS group and 1 of the 53 in the CS group. The labyrinth was damaged in four patients in the MS group but was not damaged in any of the CS group patients. The CS group included 29 lateral type and 24 medial type schwannomas. Endoscopic-assisted resection of residual tumor in the IAC was performed significantly more often on medial than on lateral tumors.

Conclusion: Navigation and endoscopy are useful in assisting the exposure of the intrameatal portion of VSs. Preoperative MRI/CT fusion imaging is helpful in preoperative evaluation and surgical planning in patients undergoing VS surgery. Tumors of the medial type require endoscopic assistance for resection.
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http://dx.doi.org/10.3389/fsurg.2022.889402DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9274124PMC
June 2022

Anti-diabetic effect of banana peel dietary fibers on type 2 diabetic mellitus mice induced by streptozotocin and high-sugar and high-fat diet.

J Food Biochem 2022 Jun 29:e14275. Epub 2022 Jun 29.

Engineering Research Center of Bio-Process, Ministry of Education, Hefei University of Technology, Hefei, Anhui, PR China.

We used a high-fat diet (HFD) and streptozotocin (STZ) to induce type 2 diabetic mellitus (T2DM) mice and evaluated the effect of banana peel dietary fibers (BP-DFs) as potential hypoglycemic agents. After 5 weeks of intervention with banana peel dietary fibers (BP-DFs), food intake was reduced, body weight was increased, blood lipids and glucose were reduced, fasting insulin and GLP-1 levels were increased, and liver and pancreatic tissue damage was reduced. Banana peel soluble dietary fiber (BP-SDF) has the most significant effect. The results of fecal microbiota analysis showed that BP-DFs could ameliorates gut microbiome dysbiosis, and all three types of dietary fibers have obvious effects. The results of fecal short-chain fatty acids (SCFAs) showed that the content of fecal SCFAs was increased after BP-DFs dietary intervention, and BP-SDF had the most obvious effect. RT-PCR experiment results show that BP-DFs can up-regulate the mRNA expression levels of PI3K, AKT, IRS-1, and FOXO1 in the liver of diabetic mice, which indicates that BD-DFs may play a role in improving insulin resistance and insulin signal transduction via the IRS/PI3K/AKT pathway, improving insulin resistance and insulin signal transduction. Our research may be extended to BP-DFs, especially BP-SDF, as the basis for potential dietary intervention to prevent or treat type 2 diabetic mellitus. This work supports future research studies of the anti-diabetic properties of BP-SDF in humans. PRACTICAL APPLICATIONS: Diabetes can lead to a variety of complications that have a huge impact on health. Dietary fiber may help in lowering blood sugar. Our experimental results showed that banana peel dietary fibers have the effect of reducing food intake, blood sugar, improving liver and pancreas function, increasing the abundance of intestinal flora, and improving the IRS/PI3K/AKT pathway in T2DM mice. Therefore, this study could provide a theoretical basis for the development of functional foods with banana peel dietary fiber.
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http://dx.doi.org/10.1111/jfbc.14275DOI Listing
June 2022

Correlation Between Ictal Signs and Anatomical Subgroups in Temporal Lobe Seizures: A Stereoelectroencephalography Study.

Front Neurol 2022 10;13:917079. Epub 2022 Jun 10.

Department of Neurosurgery, Center of Epilepsy, Beijing Institute for Brain Disorders, Beijing Key Laboratory of Epilepsy Research, Sanbo Brain Hospital, Capital Medical University, Beijing, China.

Objective: Ictal semiology is a fundamental part of the presurgical evaluation of patients with temporal lobe epilepsy. We aimed to identify different anatomical and semiologic subgroups in temporal lobe seizures, and investigate the correlation between them.

Methods: We enrolled 93 patients for whom stereoelectroencephalography exploration indicated that the seizure-onset zone was within the temporal lobe. Ictal signs and concomitant stereoelectroencephalography changes were carefully reviewed and quantified, and then cluster analysis and the Kendall correlation test were used to associate ictal signs with the temporal structures of patients.

Results: Clustering analysis identified two main groups of temporal structures. Group 1 consisted of the medial temporal lobe structures and the temporal pole, which were divided into two subgroups. Group 1A included the hippocampal head, hippocampal body, and amygdala, and this subgroup correlated significantly with oroalimentary automatisms, feeling of fear, and epigastric auras. Group 1B included the hippocampal tail, temporal pole, and parahippocampal gyrus, and this subgroup correlated significantly with manual and oroalimentary automatisms. Group 2 consisted of the cortical structures of the temporal lobe and was also divided into two subgroups. Group 2A included the superior and middle temporal gyrus, correlated significantly with bilateral rictus/facial contraction, generalized tonic-clonic seizure, and manual automatisms. Group 2B included Heschl's gyrus, the inferior temporal gyrus, and the fusiform gyrus, and this subgroup correlated significantly with auditory auras, focal hypokinetics, unilateral upper and lower limbs tonic posture/clonic signs, head/eye deviation, unilateral versive signs, and generalized tonic-clonic seizure.

Significance: The temporal structures can be categorized according to the level at which each structure participates in seizures, and different anatomical subgroups can be correlated with different ictal signs. Identifying specific semiologic features can help us localize the epileptogenic zone and thus develop stereoelectroencephalography electrode implantation and surgical resection protocols for patients with temporal lobe epilepsy.
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http://dx.doi.org/10.3389/fneur.2022.917079DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9226566PMC
June 2022

Intraoperative Findings of Inferior Petrosal Vein During Microvascular Decompression for Hemifacial Spasm: A Single-Surgeon Experience.

Front Surg 2022 10;9:921589. Epub 2022 Jun 10.

Department of Neurosurgery, Zhongnan Hospital of Wuhan University, Wuhan, China.

Objective: This study aims to evaluate the impact of the inferior petrosal veins (IPVs) on operational exploration and to analyze related anatomic features.

Methods: A total of 317 patients were retrospectively studied. Surgical outcomes and postoperative complications were analyzed, and patients were divided into two groups according to whether the IPV was sacrificed or preserved. The diameter of the IPV was also recorded during operation. Furthermore, the position where the IPV drained into the jugular bulb was recorded in each patient, and the influence of different injection points on the operation was analyzed.

Results: IPVs were conclusively identified in 242/317 (76.3%) of patients, with 110/242 (45.5%) of patients categorized as "IPV sacrifice" versus 132/242 (54.5%) categorized as "IPV preservation." IPV diameter was observed to be <0.5 mm in 58 cases (23.9%), 0.5 mm-1.0 mm (≥0.5 mm and ≤1.0 mm) in 145 cases (59.9%), and >1 mm in 39 cases (16.2%). The position of IPV drainage into the jugular bulb was at the level of the accessory nerve in 163 cases (67.3%), the level of the vagus nerve in 42 cases (17.4%), and the level of the glossopharyngeal nerve or above in 37 cases (15.3%). The diameters of IPV in the sacrifice group were mainly less than 1 mm (94.5% vs. 75%, < 0.01), and the cases with draining points near the glossopharyngeal nerve were more than that in the preservation group (27.3% vs. 5.3%,  < 0.01).

Conclusion: IPV is an obstructive structure in MVD for HFS, with considerable variations in diameters and draining points. IPV near the glossopharyngeal nerve significantly impacts surgical exposure and is often sacrificed for a better view of the operation field. Meanwhile, it is feasible to maintain IPVs with a diameter >1 mm.
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http://dx.doi.org/10.3389/fsurg.2022.921589DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9226572PMC
June 2022

Corynoline protects ang II-induced hypertensive heart failure by increasing PPARα and Inhibiting NF-κB pathway.

Biomed Pharmacother 2022 Jun 5;150:113075. Epub 2022 May 5.

Key Laboratory of Natural Medicines of the Changbai Mountain, Ministry of Education, Yanbian University, Yanji 133002, PR China; School of Pharmaceutical Sciences, Hangzhou Medical College, Hangzhou, Zhejiang 311399, PR China. Electronic address:

Heart failure is a fairly common outcome of hypertension. Recent studies have highlighted the key role of the non-hemodynamic activity of angiotensin II (Ang II) in hypertensive heart failure via inducing cardiac inflammation. Drugs that disrupt Ang II-induced cardiac inflammation may have clinical utility in the treatment of hypertensive heart failure. A naturally occurring compound, corynoline, exhibit anti-inflammatory activities in other systems. C57BL/6 mice were injected with Ang II via a micro-osmotic pump for four weeks to develop hypertensive heart failure. The mice were treated with corynoline by gavage for two weeks. RNA-sequencing analysis was performed to explore the potential mechanism of corynoline. We found that corynoline could inhibit inflammation, myocardial fibrosis, and hypertrophy to prevent heart dysfunction, without the alteration of blood pressure. RNA-sequencing analysis indicates that the PPARα pathway is involved Ang II-induced cardiac fibrosis and cardiac remodeling. Corynoline reversed Ang II-induced PPARα inhibition both in vitro and in vivo. We further found that corynoline increases the interaction between PPARα and P65 to inhibit the NF-κB pro-inflammatory pathway in H9c2 cells. Our studies show that corynoline relieves Ang II-induced hypertensive heart failure by increasing the interaction between PPARα and P65 to inhibit the NF-κB pathway.
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http://dx.doi.org/10.1016/j.biopha.2022.113075DOI Listing
June 2022

Porous Organic Polymers via Diels-Alder Reaction for the Removal of Cr(VI) from Aqueous Solutions.

ACS Macro Lett 2022 04 16;11(4):447-451. Epub 2022 Mar 16.

College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu, 610065, People's Republic of China.

The Diels-Alder reaction is striking as a prominent synthetic tool for the rapid construction of complex molecular frameworks, but the synthesis of porous organic polymers (POPs) via the Diels-Alder reaction is rare. Herein, we report the solvothermal synthesis of a new type of POPs (DA-POPs) via the furan/alkynyl Diels-Alder reaction. These polymers show favorable porous properties and high specific surface areas (up to 1041 m·g). Meanwhile, the high porosity in conjuction of ether bridges in the DA-POPs enable a fine adsorption performance for the removal of Cr(VI) from aqueous solutions.
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http://dx.doi.org/10.1021/acsmacrolett.2c00052DOI Listing
April 2022

Evidence of Omics, Immune Infiltration, and Pharmacogenomics for BATF in a Pan-Cancer Cohort.

Front Mol Biosci 2022 29;9:844721. Epub 2022 Apr 29.

Department of Neurosurgery, Zhongnan Hospital of Wuhan University, Wuhan, China.

Cytotoxic CD8+ T-cell exhaustion is the major barrier for immunotherapy in tumors. Recent studies have reported that the basic leucine zipper activating transcription factor-like transcription factor (BATF) is responsible for countering cytotoxic CD8+ T-cell exhaustion. Nevertheless, the expression and roles of BATF in tumors have been poorly explored. In the present study, we conducted a multi-omics analysis, including gene expression, methylation status, DNA alterations, pharmacogenomics, and survival status based on data from the Cancer Genome Atlas (TCGA) database to discern expression patterns and prognostic roles of BATF in tumors. We also explored potential roles of BATF in a pan-cancer cohort by performing immune infiltration, Gene Ontology (GO) enrichment, and the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. assay was also performed to explore roles of BATF in tumor cells. We found that BATF was aberrantly upregulated in 27 types of tumors with respect to the corresponding normal tissues. Abnormal BATF expression in tumors predicted survival times of patients in a tissue-dependent manner. The results of GO, immune infiltration, and KEGG analysis revealed that increased BATF expression in tumors participated in modulating immune cell infiltration immune-related pathways. BATF expression could also predict immunotherapeutic and chemotherapy responses in cancers. Moreover, knockdown of BATF suppresses tumor cell viability. Our present study reports the vital roles of BATF in tumors and provides a theoretical basis for targeting BATF therapy.
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http://dx.doi.org/10.3389/fmolb.2022.844721DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9098817PMC
April 2022

DMF-Based Large-Grain Spanning Cu ZnSn(S ,Se ) Device with a PCE of 11.76.

Adv Sci (Weinh) 2022 Jul 28;9(20):e2201241. Epub 2022 Apr 28.

Key Laboratory of Micro-Nano Materials for Energy Storage and Conversion of Henan Province, Institute of Surface Micro and Nano Materials, College of Chemical and Materials Engineering, Xuchang University, Xuchang, Henan Province, 461000, P. R. China.

A main concern of the promising DMF-based Cu ZnSn(S ,Se ) (CZTSSe) solar cells lies in the absence of a large-grain spanning structure, which is a key factor for high open-circuit voltage (V ) and power conversion efficiency (PCE). A new strategy to achieve CZTSSe large-grain spanning monolayer is proposed, by taking advantage of the synergistic optimization with a Cu plus Sn redox system and pre-annealing temperatures. A series of structural, morphological, electrical, and photoelectric characterizations are employed to study the effects of the pre-annealing temperatures on absorber qualities, and an optimized temperature of 430 ℃ is determined. The growth mechanism of the large-grain spanning monolayer and the effect of redox reaction rate are carefully investigated. Three types of absorber growth mechanisms and a concept of critical temperature are proposed. The devices based on this large-grain spanning monolayer suppress the recombination of carriers at crystal boundaries and interfaces. The champion device exhibits a high V (>500 mV) and PCE of 11.76%, which are both the maximum values among DMF-based solar cells at the current stage.
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http://dx.doi.org/10.1002/advs.202201241DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9284129PMC
July 2022

The influencing factors and changes of cognitive function within 40 Rasmussen encephalitis patients that received a hemispherectomy.

Neurol Res 2022 Aug 17;44(8):700-707. Epub 2022 Feb 17.

Department of Neurosurgery, Sanbo Brain Hospital, Capital Medical University, Beijing, China.

Objective: To evaluate the influencing factors and cognitive functional changes in Rasmussen encephalitis (RE) patients who received a hemispherectomy.

Methods: Forty RE patients underwent a hemispherectomy with at least a 2 years follow- up were included in this study . Postoperative seizure outcomes were evaluated according to the Engle classification scale. Univariate analysis and a multivariate logistic regression model in a backward fashion were used to identify the potential predictors of cognitive function.

Results: All 40 patients had an Engle classification outcome at a 2 years follow-up. Univariate and multivariate analyses revealed that seizure duration (OR 10.06, 95% CI 1.54-3.85, p = 0.038), age at surgery (OR 3.06, 95% CI 1.21-3.56, p = 0.043), and MRI score (OR 0.09, 95% CI 0.01-0.67 p = 0.024) are associated with postoperative cognitive outcomes respectively. Moreover,  VIQ and PIQ were negatively correlated linearly with duration of seizures and MRI score. Patients with a good VIQ and PIQ before the operation were more likely to have a better VIQ and PIQ postoperatively (p < 0.001 and p < 0.001, respectively). And, operation side is an important factor affecting cognitive function; therefore, a left hemispherectomy has a greater impact on the patient's IQ and language.

Conclusions: RE patients' cognitive dysfunctions are improved after a hemispherectomy. Right-side operation can achieve better postoperative cognitive outcomes especially in VIQ and language. A shorter duration of seizures, early age at surgery, and less severe brain atrophy suggest better cognitive outcomes after a hemispherectomy.
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http://dx.doi.org/10.1080/01616412.2022.2039526DOI Listing
August 2022

Enantioselective Total Synthesis of Hyperforin and Pyrohyperforin.

Angew Chem Int Ed Engl 2022 04 19;61(16):e202116136. Epub 2022 Feb 19.

State Key Laboratory of Natural and Biomimetic Drugs, Chemical Biology Center, School of Pharmaceutical Sciences, Peking University, Xue Yuan Road No. 38, Beijing, 100191, China.

Capitalizing on the late-stage diversification of an essential 1,3-diene intermediate, we describe herein a 9-step enantioselective total synthesis of (+)-hyperforin and (+)-pyrohyperforin, starting from commercially available allylacetone. Our convergent synthesis features a series of critical reactions: 1) an enantioselective deconjugative α-alkylation of α,β-unsaturated acid using chiral lithium amides as noncovalent stereodirecting auxiliaries; 2) a HfCl -mediated carbonyl α-tert-alkylation to forge the intricate bicyclo[3.3.1]nonane framework; 3) an abiotic cascade pyran formation; and 4) a selective 1,4-semihydrogenation of polyenes. During the course of our synthesis, we also identified a 1,2-cyclopropanediol overbred intermediate which was responsible for the 1,3-diene precursor formation through a controlled fragmentation.
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http://dx.doi.org/10.1002/anie.202116136DOI Listing
April 2022

Analysis of power spectrum and phase lag index changes following deep brain stimulation of the anterior nucleus of the thalamus in patients with drug-resistant epilepsy: A retrospective study.

Seizure 2022 Mar 10;96:6-12. Epub 2022 Jan 10.

Department of Neurosurgery, Sanbo Brain Hospital, Capital Medical University, Beijing 100093, China; Beijing Key Laboratory of Epilepsy, Beijing 100093, China; Epilepsy Institute, Beijing Institute for Brain Disorders, Beijing 100093, China.

Objectives: The mechanisms underlying the anterior nucleus of the thalamus (ANT) deep brain stimulation (DBS) for the treatment of drug-resistant epilepsy (DRE) have not been fully explored. The present study aimed to measure the changes in whole-brain activity generated by ANT DBS using interictal electroencephalography (EEG).

Materials And Methods: Interictal EEG signals were retrospectively collected in 20 DRE patients who underwent ANT DBS surgery. Patients were classified as responders or non-responders depending on their response to ANT DBS treatment. The power spectrum (PS) and Phase Lag Index (PLI) were determined and data analyzed using a paired sample t-test to evaluate activity differences between pre-and-post-treatment on different frequency categories. Student's t-test, Mann-Whitney test (non-parametric test) and Fisher exact test were used to compare groups in terms of clinical variables and EEG metrics. P values < 0.05 were considered statistically significant, and FDR-corrected values were used for multiple testing.

Results: PS analysis revealed that whole-brain spectral power had a significant decrease in the beta (p = 0.005) and gamma (p = 0.037) bands following ANT DBS treatment in responders. The analysis of scalp topographic images of all patients showed that ANT DBS decreases PS in the beta band at the F3, F7 and Cz electrode sites. The findings indicated a decrease in PS in the gamma band at the Fp2, F3, Cz, T3, T5 and Oz electrode sites. After ANT DBS treatment, PLI analysis showed a significant decrease in PLI between Fp1 and T3 in the gamma band in responders.

Conclusion: The findings showed that ANT DBS induces a decrease in power in the left frontal lobe, left temporal lobe and midline areas in the beta and gamma bands. Lower whole-brain power in the beta and gamma bands can be used as biomarkers for a favorable therapeutic response to ANT DBS, and decreased synchronization between the left frontal pole and temporal lobe in the gamma band can also be used as a biomarker for effective clinical stimulation to guide postoperative programming.
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http://dx.doi.org/10.1016/j.seizure.2022.01.004DOI Listing
March 2022

Progressive multifocal leukoencephalopathy and peripheral neuropathy in a patient with Good's syndrome.

J Neurovirol 2022 02 17;28(1):177-180. Epub 2022 Jan 17.

Department of Neurology, Sanbo Brain Hospital,Capital Medical University, Haidian District, 50, Xiang-shan-yi-ke-song, 100093, Beijing, China.

Good's syndrome (GS) is an immunodeficiency characterized by thymoma, hypogammaglobulinemia, and impaired T-cell function. Progressive multifocal encephalopathy (PML), an infection caused by JC virus (JCV), usually occurs in patients infected with human immunodeficiency virus (HIV), or in patients on treatment with immunosuppressive or immunomodulatory drugs. There were few reports of PML due to GS, especially with the comorbidity of peripheral neuropathy. We describe a case of an uncommon presentation of PML and peripheral neuropathy in a male who presented with blurred vision, cognitive changes, limb weakness, and numbness over a 4-month period due to GS. To the best of our knowledge, this is the first report of PML and peripheral neuropathy due to GS. This case aims to highlight that it is necessary to consider the possibility of PML due to GS in patients with thymoma and intracranial lesions, and we should focus not only on opportunistic infections of the central nervous system, such as PML, but also on peripheral neuropathy.
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http://dx.doi.org/10.1007/s13365-021-01041-4DOI Listing
February 2022

Characteristics, surgical outcomes, and influential factors of epilepsy in Sturge-Weber syndrome.

Brain 2021 Dec 21. Epub 2021 Dec 21.

Department of Neurosurgery, SanBo Brain Hospital, Capital Medical University, Beijing 100093, China.

Few studies have reported the clinical presentation, surgical treatment, outcomes, and influential factors for patients with epilepsy and Sturge-Weber syndrome. This large-scale retrospective study continuously enrolled 132 patients with Sturge-Weber syndrome and epilepsy from January 2008 to December 2018 at our hospital to analyze their characteristics. Among these patients, 90 underwent epilepsy surgery, and their postoperative 2-year follow-up seizure, cognitive, and motor functional outcomes were assessed and analyzed. Univariable and multivariable logistic analyses were conducted to explore the influential factors. Among the Sturge-Weber syndrome patients for whom characteristics were analyzed (n = 132), 76.52% of patients had their first epileptic seizures within their first year of life. The risk factors for cognitive decline were seizure history≥2 years (adjusted odds ratio [aOR] = 3.829, 95% confidence interval [CI]: 1.810-9.021, p = 0.008), bilateral leptomeningeal angiomas (aOR = 3.173, 95% CI: 1.970-48.194, p = 0.013), age at onset < 1 year (aOR = 2.903, 95% CI: 1.230-6.514, p = 0.013), brain calcification (aOR = 2.375, 95% CI: 1.396-5.201, p = 0.021) and left leptomeningeal angiomas (aOR = 2.228, 95% CI: 1.351-32.571, p = 0.030). Of the patients who underwent epilepsy surgery (n = 90), 44 were subject to focal resection, and 46 underwent hemisphere surgery (19 anatomical hemispherectomies and 27 modified hemispherotomies). A postoperative seizure-free status, favorable cognitive outcomes, and favorable motor outcomes were achieved in 83.33%, 44.44%, and 43.33% of surgical patients, respectively. The modified hemispherotomy group had similar surgical outcomes, less intraoperative blood loss and shorter postoperative hospital stays than the anatomical hemispherectomy group. Regarding seizure outcomes, full resection (aOR = 11.115, 95% CI: 1.260-98.067, p = 0.020) and age at surgery < 2 years (aOR = 6.040, 95% CI: 1.444-73.367, p = 0.031) were positive influential factors for focal resection. Age at surgery < 2 years (aOR = 15.053, 95% CI: 1.050-215.899, p = 0.036) and infrequent seizures (aOR = 8.426, 95% CI: 1.086-87.442, p = 0.042; monthly vs. weekly) were positive influential factors for hemisphere surgery. In conclusion, epilepsy surgery resulted in a good postoperative seizure-free rate and favorable cognitive and motor functional outcomes and showed acceptable safety for patients with epilepsy and Sturge-Weber syndrome. Modified hemispherotomy is a less invasive and safer type of hemisphere surgery than traditional anatomic hemispherectomy with similar surgical outcomes. Early surgery may be helpful to achieve better seizure outcomes and cognitive protection, while the risk of surgery for young children should also be considered.
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http://dx.doi.org/10.1093/brain/awab470DOI Listing
December 2021

Magnetoencephalography STOUT Method Adapted to Radiofrequency Thermocoagulation for MR-Negative Insular Epilepsy: A Case Report.

Front Neurol 2021 13;12:683299. Epub 2021 Oct 13.

Center for MRI Research, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, China.

Epilepsy is one of the most challenging neurologic diseases confronted by human society. Approximately 30-40% of the worldwide epilepsy patients are diagnosed with drug-resistant epilepsy and require pre-surgery evaluation. Magnetoencephalography (MEG) is a unique technology that provides optimal spatial-temporal resolution and has become a powerful non-invasive imaging modality that can localize the interictal spikes and guide the implantation of intracranial electrodes. Currently, the most widely used MEG source estimation method for clinical applications is equivalent current dipoles (ECD). However, ECD has difficulties in precisely locating deep sources such as insular lobe. In contrast to ECD, another MEG source estimation method named spatio-temporal unifying tomography (STOUT) with spatial sparsity has particular advantages in locating deep sources. In this case study, we recruited a 5 year-old female patient with insular lobe epilepsy and her seizure recurred in 1 year after receiving the radiofrequency thermocoagulation (RF-TC) therapy. The STOUT method was adopted to locate deep sources for identifying the epileptic foci in epilepsy evaluation. MEG STOUT method strongly supported a stereo-electroencephalographic (SEEG)-guided RF-TC operation, and the patient reported a satisfactory therapeutic effect. This case raises the possibility that STOUT method can be used particularly for the localization of deep sources, and successfully conducted RF-TC under the guidance of MEG STOUT results.
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http://dx.doi.org/10.3389/fneur.2021.683299DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8548742PMC
October 2021

Cosmc transfection decreases malignant behavior of Tn cells and enhances sensitivity to apoptosis when induced by Apo2L/TRAIL via alteration of O-glycan structure.

Aging (Albany NY) 2021 10 13;13(19):23393-23406. Epub 2021 Oct 13.

Department of Immunology, Binzhou Medical University, Yantai 264003, PR China.

Cosmc mutations may cause abnormal O-glycosylation and result in Tn antigen expression. In the current study, it was discovered that proliferation and migration of Tn+ cells (Jurkat T and LS174T-Tn cells) with mutant Cosmc decreased after transfected Cosmc, and their sensitivity to apoptosis induced by Apo2L/TRAIL increased. Core 1-, 2-, and 3-derived O-glycans were absent in Tn cells. After Cosmc transfection, normal extended core 1-derived O-glycans appeared and were accompanied by increased T-synthase activity. Core 2-derived O-glycans appeared in transfected LS174T-Tn cells, and their structural types and levels were lower than those in LS174T-Tn cells. Core 3-derived O-glycans were present only in LS174T-Tn cells. The activity of C3GnT in LS174T-Tn cells was lower than that in LS174T-Tn cells, and it was absent in Jurkat T cells. Cosmc transfection did not alter C3GnT activity or core 3-derived O-glycans in Jurkat T and LS174T-Tn cells. The results demonstrated that the composition and structure of O-glycans were different among various Tn cells, which not only affected cell malignant behavior but also modulated sensitivity to apoptotic stimuli. Thus, Cosmc transfection may effectively decrease the malignant behavior of Tn tumor cells and enhance their sensitivity to apoptosis when induced by Apo2L/TRAIL through modification of O-glycans.
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http://dx.doi.org/10.18632/aging.203633DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8549606PMC
October 2021

Generation of Tetrafluoroethylene-Propylene Elastomer-Based Microfluidic Devices for Drug Toxicity and Metabolism Studies.

ACS Omega 2021 Sep 15;6(38):24859-24865. Epub 2021 Sep 15.

Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto 606-8507, Japan.

Polydimethylsiloxane (PDMS) is widely used to fabricate microfluidic organs-on-chips. Using these devices (PDMS-based devices), the mechanical microenvironment of living tissues, such as pulmonary respiration and intestinal peristalsis, can be reproduced . However, the use of PDMS-based devices in drug discovery research is limited because of their extensive absorption of drugs. In this study, we investigated the feasibility of the tetrafluoroethylene-propylene (FEPM) elastomer to fabricate a hepatocyte-on-a-chip (FEPM-based hepatocyte chip) with lower drug absorption. The FEPM-based hepatocyte chip expressed drug-metabolizing enzymes, drug-conjugating enzymes, and drug transporters. Also, it could produce human albumin. Although the metabolites of midazolam and bufuralol were hardly detected in the PDMS-based hepatocyte chip, they were detected abundantly in the FEPM-based hepatocyte chip. Finally, coumarin-induced hepatocyte cytotoxicity was less severe in the PDMS-based hepatocyte chip than in the FEPM-based hepatocyte chip, reflecting the different drug absorptions of the two chips. In conclusion, the FEPM-based hepatocyte chip could be a useful tool in drug discovery research, including drug metabolism and toxicity studies.
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http://dx.doi.org/10.1021/acsomega.1c03719DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8482466PMC
September 2021

Aberrant Expression of Circulating MicroRNA Leads to the Dysregulation of Alpha-Synuclein and Other Pathogenic Genes in Parkinson's Disease.

Front Cell Dev Biol 2021 23;9:695007. Epub 2021 Aug 23.

Department of Neurosurgery, Zhongnan Hospital of Wuhan University, Wuhan, China.

A group of circulating microRNAs (miRNAs) have been implicated in the pathogenesis of Parkinson's disease. However, a comprehensive study of the interactions between pathogenic miRNAs and their downstream Parkinson's disease (PD)-related target genes has not been performed. Here, we identified the miRNA expression profiles in the plasma and circulating exosomes of Parkinson's disease patients using next-generation RNA sequencing. Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) analyses showed that the miRNA target genes were enriched in axon guidance, neurotrophin signaling, cellular senescence, and the Transforming growth factor-β (TGF-β), mitogen-activated protein kinase (MAPK), phosphatidylinositol 3-kinase (PI3K)-protein kinase B (AKT) and mechanistic target of rapamycin (mTOR) signaling pathways. Furthermore, a group of aberrantly expressed miRNAs were selected and further validated in individual patient plasma, human neural stem cells (NSCs) and a rat model of PD. More importantly, the full scope of the regulatory network between these miRNAs and their PD-related gene targets in human neural stem cells was examined, and the findings revealed a similar but still varied downstream regulatory cascade involving many known PD-associated genes. Additionally, miR-23b-3p was identified as a novel direct regulator of alpha-synuclein, which is possibly the key component in PD. Our current study, for the first time, provides a glimpse into the regulatory network of pathogenic miRNAs and their PD-related gene targets in PD. Moreover, these PD-associated miRNAs may serve as biomarkers and novel therapeutic targets for PD.
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http://dx.doi.org/10.3389/fcell.2021.695007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8419519PMC
August 2021

Scalp-HFO indexes are biomarkers for the lateralization and localization of the epileptogenic zone in preoperative assessment.

J Neurophysiol 2021 10 8;126(4):1148-1158. Epub 2021 Sep 8.

Department of Neurology, Sanbo Brain Hospital, Capital Medical University, Beijing, China.

During the noninvasive evaluation phase for refractory epilepsy, the localization of the epileptogenic zone (EZ) is essential for the surgical protocols. Confirmation of laterality is required when the preoperative evaluation limits the EZ to bilateral anterior temporal lobes or bilateral frontal lobes. High-frequency oscillations (HFOs) are considered to be promising biological markers for the EZ. However, a large number of studies on HFOs stem from intracranial research. There were few quantitative measures for scalp HFOs, so we proposed a new method to quantify and analyze scalp HFOs. This method was called the "scalp-HFO index" (HI) and calculated in both the EZ and non-EZ. The calculation was based on the numbers and spectral power of scalp HFOs automatically detected. We labeled the brain lobes involved in the EZ as regions of interest (ROIs). The HIs based on the ripple numbers (n-HI) and spectral power (s-HI) were significantly higher in the ROI than in the contra-ROI ( = 0.012, = 0.003), indicating that HIs contributed to the lateralization of EZ. The sensitivity and specificity of n-HI for the localization of the EZ were 90% and 79.58%, respectively, suggesting that n-HI was valuable in localizing the EZ. HI may contribute to the implantation strategy of invasive electrodes. However, few scalp HFOs were recorded when the EZ was located in the medial cortex region. We proposed the scalp-high-frequency oscillation (HFO) index (HI) as a quantitative assessment method for scalp HFOs to locate the epileptogenic zone (EZ). Our results showed that the HI in regions of interest (ROIs) was significantly higher than in contra-ROIs. Sensitivity and specificity of HI based on ripple rates (n-HI) for EZ localization were 90% and 79.58%, respectively. If the n-HI of the brain region was >1.35, it was more likely to be an epileptogenic region. Clinical application of HIs as an indicator may facilitate localization of the EZ.
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http://dx.doi.org/10.1152/jn.00212.2021DOI Listing
October 2021

Nanoparticles of a New Small-Molecule P-Selectin Inhibitor Attenuate Thrombosis, Inflammation, and Tumor Growth in Two Animal Models.

Int J Nanomedicine 2021 24;16:5777-5795. Epub 2021 Aug 24.

School of Pharmaceutical Sciences, Beijing Area Major Laboratory of Peptide and Small Molecular Drugs, Engineering Research Center of Endogenous Prophylactic of Ministry of Education of China, Capital Medical University, Beijing, 100069, People's Republic of China.

Purpose: To assess whether the newly designed small-molecule oral P-selectin inhibitor 3-1,2,3,4-tetrahydro-β-carboline-3-methyl aspartyl ester (THCMA) as a nanomedicine enhances antithrombosis, anti-inflammation, and antitumor activity more than the clinical trial drug PSI-697.

Methods: THCMA was designed as an amphiphile containing pharmacophores of PSI-697. Its nanofeatures were explored with TEM, SEM, Tyndall effect, ζ-potential, FT-ICR-MS, and NOESY 2D H NMR. The P-selectin inhibitory effect of THCMA was demonstrated with molecular docking, ultraviolet (UV) spectra, and competitive ELISA. In vivo and in vitro assays - anti-arterial thrombosis, anti-venous thrombosis, anti-inflammation, antitumor growth, anti-platelet aggregation, rat-tail bleeding time, anticoagulation index, soluble P-selectin (sP-selectin) expression, and serum TNFα expression - were performed to explore bioactivity and potential mechanisms. Water solubility of THCMA was measured using UV-absorption spectra.

Results: THCMA self-assembled into nanorings of approximately 100 nm in diameter. Its water solubility was about 1,030-fold that of PSI-697. THCMA exhibited more potent P-selectin inhibitory effect than PSI-697. The oral efficacy of THCMA was 100-fold that of PSI-697 in inhibiting arterial and venous thrombosis and tenfold in inhibiting inflammation. THCMA inhibited thrombosis at a dose that produces no coagulation disorders and no bleeding risk. THCMA exhibited enhanced antitumor activity over PSI-697 without systemic chemotherapy toxicity. THCMA significantly inhibited platelet aggregation in vitro and downregulated the expression levels of serum sP-selectin and TNFα in vivo.

Conclusion: A new small-molecule P-selectin inhibitor, THCMA, has been successfully designed as a nanomedicine with largely enhanced oral efficacy compared to the clinical trial drug PSI-697, and thus might be developed for the oral treatment of arterial thrombosis, venous thrombosis, inflammation, and cancer-associated thrombosis.
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http://dx.doi.org/10.2147/IJN.S316863DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8403725PMC
November 2021

Homogeneous deacetylation and degradation of chitin in NaOH/urea dissolution system.

Int J Biol Macromol 2021 Oct 24;189:391-397. Epub 2021 Aug 24.

College of Marine Life Science, Ocean University of China, 5# Yushan Road, Qingdao 266003, Shandong Province, China; Qingdao National Laboratory for Marine Science and Technology, Qingdao 266000, China. Electronic address:

Since being discovered, alkali/urea has been widely used in the dissolution of natural polysaccharides and the preparation of functional materials such as hydrogels, fibers, films and nanoparticles. This work will focus on verifying the structural stability, homogeneous degradation and deacetylation of chitin in alkali-soluble systems. The chitin was dissolved in NaOH/urea solution and stored at different temperature. At the specific time, the structure, viscosity, acetylation degree (DA) and biocompatibility of chitin and prepared chitosan were determined. The results indicated that dissolution process did not affect the structure and bioactivity of chitin. However, with the increase of storage time and temperature, chitin undergone significant homogeneous deacetylation (DA from 99.5% to 33.2%) and degradation (viscosity from 9284 cP to 1538 cP), accompanying by changes in crystalline structure and thermal stability. Moreover, the processed chitins were no-toxic for the biomedicine applications. This work will provide new ideas for the application of alkali-soluble systems.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.08.126DOI Listing
October 2021

discovery of SARS-CoV-2 main protease inhibitors from the carboline and quinoline database.

Future Virol 2021 Jul 20. Epub 2021 Jul 20.

Beijing Area Major Laboratory of Peptide & Small Molecular Drugs, Engineering Research Center of Endogenous Prophylactic of Ministry of Education of China, Beijing Laboratory of Biomedical Materials, College of Pharmaceutical Sciences of Capital Medical University, Beijing, 100069, China.

SARS-CoV-2 caused more than 3.8 million deaths according to the WHO. In this urgent circumstance, we aimed at screening out potential inhibitors targeting the main protease of SARS-CoV-2. An in-house carboline and quinoline database including carboline, quinoline and their derivatives was established. A virtual screening in carboline and quinoline database, 50 ns molecular dynamics simulations and molecular mechanics Poisson-Boltzmann surface area calculations were carried out. The top 12 molecules were screened out preliminarily. The molecular mechanics Poisson-Boltzmann surface area ranking showed that p59_7m, p12_7e, p59_7k stood out with the lowest binding energies of -24.20, -17.98, -17.67 kcal/mol, respectively. The study provides powerful results that indicate the selected molecules are valuable for further evaluation as SARS-CoV-2 main protease inhibitors.
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http://dx.doi.org/10.2217/fvl-2021-0099DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8293686PMC
July 2021

Prognostic value of histopathologic pattern for long-term surgical outcomes of 198 patients with confirmed mesial temporal lobe epilepsy.

Hum Pathol 2021 09 10;115:47-55. Epub 2021 Jun 10.

Department of Neurosurgery, Sanbo Brain Hospital, Capital Medical University, Beijing, 100093, China; Beijing Key Laboratory of Epilepsy, 100093, China. Electronic address:

Hippocampal sclerosis (HS) is the most common neuropathologic findings in patients with intractable temporal lobe epilepsy (TLE). The international league against epilepsy has proposed a new classification of HS based on pyramidal cell loss on different subfields to facilitate the study of HS pathology in patients after anterior temporal lobectomy (ATL), and the influence of these HS patterns on the prognosis of patients with TLE is contradictory. This study aims to investigate the relationship between different HS subtypes and postoperative seizure outcomes for intractable patients with TLE. From January 2008 to December 2018, we retrospectively reviewed 198 TLE patients with ATL surgery, and all patients had a complete preoperative evaluation, a specimen of hippocampal tissue after surgery, cognitive test after surgery, and more than 2 years of postoperative follow-up. The main findings were as follows: 1) temporal neocortical gray matter heterotopia were more common in the no-HS group; 2) HS type 1 was associated with a longer duration of epilepsy; 3) history of meningitis was the independent predictor of HS type 1; 4) no-HS patients experienced worse postoperative seizure outcomes than those with HS type1 and type 2, whereas no difference in seizure outcomes was obtained between HS type 1 and type 2; 5) no-HS patients were at increased risk for verbal memory decline after left hippocampal resection. The HS subtypes were associated with the prognosis of patients with TLE, and other variables were the predictors of different HS types. `Further study was to identify the HS subtypes by noninvasive evaluation to approve better postoperative outcomes.
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http://dx.doi.org/10.1016/j.humpath.2021.05.011DOI Listing
September 2021

An innovative approach to identify environmental variables with conservation priorities in habitat patches.

J Environ Manage 2021 Aug 20;292:112788. Epub 2021 May 20.

Tianjin Engineering Center of Urban River Eco-Purification Technology, School of Environmental Science and Engineering, Tianjin University, Tianjin, 300350, China. Electronic address:

Conservation of habitat patches and the related environment benefits both the focal species and human well-being. Many indices use the dispersal range to identify habitat patches with conservation priorities. However, there lacks approaches to identify environmental variables with conservation priorities (noted as target variables) in those identified patches. Therefore, this paper proposes an approach to identify environmental variables with conservation priorities in habitat patches using perception range and introduces the related assumption. It is assumed the agents select habitats based on their prior preference and perceived information in their perception ranges, which avoids the omniscient assumption of agents. Based on such assumptions, the proposed approach identifies the target variables by approximating how animals identify their habitats. It highlights the use of perception range and identifies target variables using the maximum information gain. The variables that contribute the largest reduction of uncertainty are regarded as the target variables in the habitat patches. Taking the Common Moorhen (Gallinula chloropus) living in Tianjin, China as the case, different scenarios with 100 m, 250 m and 500 m perception ranges are designed to illustrate the feasibility of the proposed approach. The proposed approach identifies the normalized vegetation index, rather than the distance to water surface, is the target variable in 42.3%, 58.9% and 72.1% habitat patches with given perception ranges. Adjustments are made on areas within the given perception range of each patch. More grid cells that has increased suitability index can be found in scenarios given 250 m perception range, which indicates the conservation area is not always the large the better. Optimizations are expected on both a better approximation method and a more thorough hypothesis of using perception range.
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http://dx.doi.org/10.1016/j.jenvman.2021.112788DOI Listing
August 2021

[Advances in the biosynthesis of natamycin and its regulatory mechanisms].

Sheng Wu Gong Cheng Xue Bao 2021 Apr;37(4):1107-1119

College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, Henan, China.

Natamycin is a polyene macrolide antibiotics with strong and broad spectrum antifungal activity. It not only effectively inhibits the growth and reproduction of fungi, but also prevents the formation of some mycotoxins. Consequently, it has been approved for use as an antifungal food preservative in most countries, and is also widely used in agriculture and healthcare. Streptomyces natalensis and Streptomyces chatanoogensis are the main producers of natamycin. This review summarizes the biosynthesis and regulatory mechanism of natamycin, as well as the strategies for improving natamycin production. Moreover, the future perspectives on natamycin research are discussed.
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http://dx.doi.org/10.13345/j.cjb.200394DOI Listing
April 2021

Quantitative evaluation of the antibacterial effectiveness and efficiency of chitosan considering the effect of neutralization.

Carbohydr Polym 2021 Aug 5;265:117918. Epub 2021 Mar 5.

College of Marine Life Science, Ocean University of China, Qingdao, 266003, China; Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China. Electronic address:

In the present research, an optimized liquid medium which had no neutralizing effect to chitosan was developed. Moreover, magnesium chloride (MgCl) was identified to be able to absolutely neutralize the antibacterial activity of chitosan and its derivatives. Took the two results together, the minimum bactericidal concentration (MBC) and minimum inhibitory concentration (MIC) of chitosan were precisely quantified through a further improved method based on the optimized medium and the relation curve between antibacterial rate and reaction time was obtained with the help of MgCl neutralizer. The MBC and MIC of chitosan were all 30 μg/mL against Staphylococcus aureus and Escherichia coli, and 100 μg/mL of chitosan acetate could reach 100 % of antibacterial rate within 3 min. Furthermore, coordination between magnesium ions and chitosan as well as reduced zeta potential of chitosan caused by coordination were inferred to be the neutralizing mechanism of MgCl neutralizer.
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http://dx.doi.org/10.1016/j.carbpol.2021.117918DOI Listing
August 2021

Corrigendum to "Evaluation of structure transformation and biocompatibility of chitosan in alkali/urea dissolution system for its large-scale application" [Int. J. Biol. Macromol. Volume 154, 1 July 2020, pages 758-764].

Int J Biol Macromol 2021 Aug 27;184:1065. Epub 2021 Apr 27.

College of Marine Life Science, Ocean University of China, 5# Yushan Road, Qingdao 266003, Shandong Province, China; Qingdao National Laboratory for Marine Science and Technology, Qingdao 266000, China. Electronic address:

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http://dx.doi.org/10.1016/j.ijbiomac.2021.03.200DOI Listing
August 2021

Predictors and Long-term Outcome of Resective Epilepsy Surgery in Patients with Tuberous Sclerosis Complex: A Single-centre Retrospective Cohort Study.

Seizure 2021 May 25;88:45-52. Epub 2021 Mar 25.

Department of Neurosurgery, Center of Epilepsy, Sanbo Brain Hospital, Capital Medical University, XiangshanYikesong 50, Haidian District, Beijing 100093, China. Electronic address:

Objective: To identify prognostic factors and long-term seizure outcomes for patients with tuberous sclerosis complex (TSC) who underwent resective treatment for epilepsy.

Methods: We enrolled 81 patients with TSC who had undergone resective epilepsy surgery at Sanbo Brain Hospital, Capital Medical University, between April 2004 and June 2019. We estimated cumulative probability of remaining seizure-free and plotted survival curves. Variables were compared using Mann-Whitney U, Pearson's correlation, continuity correction, and Fisher's exact chi-square tests. Prognostic predictors were analysed using log-rank (Mantel-Cox) tests and Cox regression models.

Results: At the last follow-up, 48 (59.3%) patients were classified as International League Against Epilepsy Class 1 (including 14 patients who had seizures <3 times postoperatively on the same or different day and were seizure-free at all other times). The estimated cumulative probability of remaining seizure-free postoperatively was 69.0% (95% confidence interval [CI] 58.8-79.2%), 61.9% (95% CI 51.1-72.7%), and 55.0% (95% CI 42.8-67.2%) at 2, 5, and 10 years, respectively. The mean time of remaining seizure-free was 7.24 ± 0.634 years (95% CI 6.00-8.49); en bloc resection was an essential positive predictor of postoperative seizure freedom, as was age at seizure onset, regional interictal video-electroencephalography pattern, and temporal lobe surgery. The longer the seizure-free time, the less likely a relapse. Patients who postoperatively experienced seizures remained likely to recover.

Conclusions: We demonstrated the efficacy of surgical treatment for patients with TSC and intractable epilepsy. Detailed perioperative tests are a reliable predictor of postoperative seizure freedom.
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http://dx.doi.org/10.1016/j.seizure.2021.03.022DOI Listing
May 2021

A concordance study determining language dominance between navigated transcranial magnetic stimulation and the Wada test in patients with drug-resistant epilepsy.

Epilepsy Behav 2021 04 23;117:107711. Epub 2021 Feb 23.

Department of Neurology, SanBo Brain Hospital, Capital Medical University, Beijing 100093, China. Electronic address:

Objective: It remains unclear whether transcranial magnetic stimulation (TMS) can replace the Wada test to determine language hemisphere dominance (HD). Using the Wada test as the gold standard, this study aimed to investigate the accuracy of navigated TMS (nTMS) in determining language HD.

Methods: This study enrolled nine right-handed patients with drug-resistant epilepsy. We hypothesized that application of nTMS to language-related areas of the language-dominant hemisphere would induce positive manifestation of language dysfunction (LD). To test our hypothesis, the patients were instructed to perform a visual object-naming task while nTMS was applied to the anterior (e.g., Broca's area) and posterior (e.g., Wernicke's area) regions, which are closely related to language processing. The Wada test result was used as the gold standard, and the diagnostic value of nTMS was assessed using the Kappa consistency test.

Results: The nTMS-induced LD positive rate for the bilateral anterior language areas (85.7%) was higher than that for the posterior language areas (57.1%). There was high consistency between nTMS stimulation of the left anterior and posterior language areas and the Wada test results for determining language HD. In contrast, the consistency of stimulation of the right anterior and posterior transfer sites was moderate (Kappa value = 0.545, P = 0.171) and low, respectively. For the latter, no statistical calculation was performed because stimulation of the right posterior speech area was negative in all patients compared with the Wada test results.

Conclusions: Our findings revealed that using nTMS to stimulate language-related left anterior and posterior areas could predict language HD with high accuracy. When the stimulation performance of these areas is positive, nTMS and the Wada test are equally accurate. Observing only negative performance may indicate that language HD has been transferred to the right side.
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http://dx.doi.org/10.1016/j.yebeh.2020.107711DOI Listing
April 2021

Multilayer calcium alginate beads containing Diatom Biosilica and Bacillus subtilis as microecologics for sewage treatment.

Carbohydr Polym 2021 Mar 5;256:117603. Epub 2021 Jan 5.

College of Marine Life Science, Ocean University of China, 5# Yushan Road, Qingdao 266003, Shandong Province, China. Electronic address:

Organic matter pollution and heavy metal pollution have become one of the main problems in water recycling, and the strategy to simultaneously remove soluble organic matter and metal ions is crucial for sewage treatment. In this study, multilayer calcium Alginate beads (n-Alg-DBs-Bas) containing Diatom Biosilica (DBs) and Bacillus subtilis (Bas) were designed as microecologics for sewage treatment. The introduction of DBs in beads and the multilayer structure could promote Bas growth, prolong the stability of the beads, and enhance the adsorption of beads, further improve the sewage treatment efficiency. The organic matter degradation of 3 layered Alg-DBs-Bas reached to 68.23 ± 0.95 % of COD and 58.88 ± 0.84 % of NH-N, and the metal ion adsorption was up to 119.31 mg/g for Fe, 110.81 mg/g for Zn and 141.34 mg/g for Cu. The prepared multilayer calcium alginate beads combined organic matter degradation and metal ions absorption, which is significant for environmental applications.
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http://dx.doi.org/10.1016/j.carbpol.2020.117603DOI Listing
March 2021
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