Publications by authors named "Mengqi Li"

109 Publications

Novel magnetic coupling constructed wetland for nitrogen removal: Enhancing performance and responses of plants and microbial communities.

Sci Total Environ 2021 Nov 29:152040. Epub 2021 Nov 29.

College of Environmental Science & Engineering, Shandong University, Qingdao 266237, PR China. Electronic address:

Vertical flow constructed wetlands (VFCWs) have been widely applied worldwide due to their small footprint and large hydraulic load, however, its sustainable operation and application is still challenging because of the unsatisfactory nitrogen removal. This study developed a novel CW coupled with a magnetic field for treating simulated wastewater, and investigated the effects of magnetic field on enhancing treatment performance and responses of wetland plants and microbial community. The results showed that the magnetic field (average 110 mT) had a significantly intensifying effect on organics and nitrogen removal. The removal efficiencies of NH-N and TN in CW exposed to magnetic field (MF-CW) were 10.14% and 9.16% higher than those in control CW (C-CW), and an increased COD removal was also found in MF-CW. Biochemical characteristics of plants indicated that the MF did not cause a severe stress for wetland plants, while MF application shifted significantly the microbial community in CWs. Relative abundances of nitrifying bacteria such as Nitrospira (2.36%), Dokdonella (0.27%) and Nitrosomonas (0.17%) had been significantly promoted due to MF exposure, and nitrification-related microbial enzyme (AMO) activity was also increased by 63%. It can be concluded that introducing MF into CWs could intensify organics and nitrogen removal via the biological process, which would contribute to a better understanding of magnetic coupling mechanism.
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http://dx.doi.org/10.1016/j.scitotenv.2021.152040DOI Listing
November 2021

-Derived Peptides Ameliorate Inflammation and Mitochondrial Dysfunction in RAW264.7 Macrophages by Modulating the NF-κB/COX-2 Pathway.

Foods 2021 Nov 3;10(11). Epub 2021 Nov 3.

National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China.

is an edible fungus that contains various bioactive substances, some of them with immunostimulatory properties. Presently, there is limited knowledge about the functional components of . Our aim was to evaluate the protective effects and molecular mechanisms of two -derived peptides, SDLKHFPF and SDIKHFPF, on lipopolysaccharide (LPS)-induced mitochondrial dysfunction and inflammation in RAW264.7 macrophages. peptides significantly ameliorated the production of inflammatory cytokines and inhibited the expression of COX-2, iNOS, IKKβ, p-IκB-α, and p-NF-κB. Immunofluorescence assays confirmed the inhibitory effect of peptides on NF-κB/p65 nuclear translocation. Furthermore, the treatment with peptides prevented the accumulation of reactive oxygen species, increased the Bcl-2/Bax ratio, reversed the loss of mitochondrial membrane potential, and rescued abnormalities in cellular energy metabolism. These findings indicate that peptides can effectively inhibit the activation of NF-κB/COX-2 and may confer an overall protective effect against LPS-induced cell damage.
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http://dx.doi.org/10.3390/foods10112680DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8621704PMC
November 2021

-derived peptide WFNNAGP protects against DSS-induced colitis by ameliorating oxidative stress and intestinal barrier dysfunction.

Food Funct 2021 Nov 29;12(23):11883-11897. Epub 2021 Nov 29.

National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, P.R. China.

Inflammatory bowel disease (IBD) is a non-specific, chronic inflammatory disease of the intestine. The precise etiology and mechanism underlying the pathogenesis of IBD have not been elucidated. In this study, we investigated the mechanisms through which the -derived peptide, WFNNAGP, exerts protective effects on the inflammatory response and oxidative stress in a dextran sodium sulfate (DSS)-induced IBD mouse model. WFNNAGP significantly attenuated colitis symptoms in mice, including weight loss, diarrhea, shortened colon, bloody stools, and histopathological changes. WFNNAGP significantly ameliorated the DSS-induced oxidative damage, showing scavenging activity against hydroxyl and DPPH radicals (23.67 ± 4.11% and 34.53 ± 2.45%), increased SOD activity (191.48 ± 4.35 U per mg prot), and decreased MDA activity (1.61 ± 0.24 nmol per mg prot). In addition, WFNNAGP improved the inflammatory response by inhibiting MPO and pro-inflammatory cytokine expression and protected the barrier function by promoting the expression of occludin and ZO-1 in the colon. Western blotting showed that WFNNAGP reduced the inflammatory response by downregulating NF-κB expression and inhibiting the formation and activation of NLRP3 and caspase-1. Thus, WFNNAGP may reduce colonic inflammation in mice by enhancing oxidative defense systems and barrier function and may be a promising candidate for IBD intervention.
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http://dx.doi.org/10.1039/d1fo02806eDOI Listing
November 2021

Formed Li-Ag Alloy Interface Enables LiGePS-Based All-Solid-State Lithium Batteries.

ACS Appl Mater Interfaces 2021 Oct 14;13(42):50076-50082. Epub 2021 Oct 14.

Key Laboratory of Material Physics, Ministry of Education, School of Physics and Microelectronics, Zhengzhou University, Zhengzhou 450001, P. R. China.

All-solid-state lithium-metal batteries (ASSLMBs) have received great interest due to their high potential to display both high energy density and safety performance. However, the poor compatibility at the Li/solid electrolyte (SE) interface and penetration of lithium dendrites during cycling strongly impede their successful commercialization. Herein, a thin Ag layer was introduced between Li and LiGePS for the formation of a Li-Ag alloy interface, thus tuning the interfacial chemistry and lithium deposition/dissolution behavior. Superior electrochemical properties and improved interfacial stability were achieved by optimizing the Ag thicknesses. The assembled symmetric cell with [email protected] 1 μm showed a steady voltage evolution up to 1000 h with an areal capacity of 1 mAh cm. Moreover, a high reversible capacity of 106.5 mAh g was achieved in an all-solid-state cell after 100 cycles, demonstrating the validity of the Ag layer. This work highlights the importance of the Li/SE interface re-engineering and provides a new strategy for improving the cycle life of ASSLMBs.
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http://dx.doi.org/10.1021/acsami.1c16356DOI Listing
October 2021

Effect of Elasticity of Silica Capsules on Cellular Uptake.

Langmuir 2021 10 30;37(40):11688-11694. Epub 2021 Sep 30.

Key Laboratory of Colloid and Interface Chemistry of the Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan, Shandong 250100, China.

Understanding the impact of the physicochemical properties of nanoparticles (NPs) on cellular uptake is important to design optimal drug-delivery nanocarriers. Therein, the influence of NP elasticity on bio-nano-interactions remains elusive due to the complexity of factors affecting cellular uptake. Herein, we synthesized SiO capsules with tunable elasticity using metal-organic frameworks as templates to investigate their interactions with cells. Young's moduli of the resultant water-filled SiO capsules with identical size, shape, composition, and surface charge can be controlled from 3.8 MPa to 4.7 GPa via the variation of capsule shell thickness. As a result, increased elasticity of SiO capsules results in higher cellular uptake. Stiff SiO capsules have almost 9 times as much cellular uptake as the soft ones. In addition, the elasticity of SiO capsules influences cellular uptake pathways, where the clathrin-mediated pathway is preferred for stiff capsules while the uptake of the soft capsules is mostly mediated by a caveolae-dependent pathway. This work confirms the important role of NP elasticity in nonspecific cell interactions, which can provide a foundational understanding for engineering drug-delivery nanocarriers.
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http://dx.doi.org/10.1021/acs.langmuir.1c01607DOI Listing
October 2021

Dextran sulfate inhibits cell proliferation and induces apoptosis by regulating EZH2 in gastric carcinoma.

Curr Cancer Drug Targets 2021 Sep 22. Epub 2021 Sep 22.

Department of Gastroenterology, Henan NO.3 Provincial People's Hospital, Zhengzhou. China.

Background: Gastric cancer (GC) is one of the most common gastrointestinal malignancies. According to reports, the enhancer of zeste homolog 2 (EZH2) exhibits carcinogenic function in a variety of cancers. Therefore, EZH2 may be a potential therapeutic target for the treatment of human cancer. Macromolecular dextran sulfate (DS) has been displayed to play a critical role in tumor inhibition. However, the molecular mechanism by which DS mediates this effect is unclear.

Objectives: In this study, we explored the effects of DS on the proliferation and apoptosis of gastric cancer and the related mechanisms. Cell proliferation and counting assays, as well as cell colony formation assays, revealed that DS inhibited the proliferation and tumorigenesis of GC cells. Additionally, flow cytometry analysis displayed that DS blocked the cell cycle of GC cells in the G1/S phase and promoted their apoptosis.

Methods: Bioinformatics analyses, enzyme-linked immunosorbent assays, immunohistochemistry, and other methods were applied to measure the expression of EZH2 in human GC cells and tissues.

Results And Discussion: Further studies have shown that DS treatment can reduce the expression of proliferating cell nuclear antigen (PCNA) and increase the level of the ratio of Bax: Bcl-2 protein in GC cells. In addition, DS reduced EZH2 levels and increased CXXC finger protein 4 levels both in vitro and in vivo. In addition, down-regulation of EZH2 with EZH2 inhibitors reversed the inhibitory effect of DS on gastric cancer cells.

Conclusion: Collectively, our work demonstrates that DS suppresses proliferation and promotes apoptosis of GC cells by regulating EZH2. Our study suggests that DS is a promising therapeutic compound for the treatment of GC.
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http://dx.doi.org/10.2174/1568009621666210922112039DOI Listing
September 2021

Virus-Inspired Gold Nanorod-Mesoporous Silica Core-Shell Nanoparticles Integrated with tTF-EG3287 for Synergetic Tumor Photothermal Therapy and Selective Therapy for Vascular Thrombosis.

ACS Appl Mater Interfaces 2021 Sep 8;13(37):44013-44027. Epub 2021 Sep 8.

Cancer Research Center, School of Medicine, Xiamen University, Xiamen 361005, Republic of China.

Synergetic therapy includes the combination of two or more conventional therapeutic approaches and can be used for tumor treatment by combining the advantages and avoiding the drawbacks of each type of treatment. In the present study, truncated tissue factor (tTF)-EG3287 fusion protein-encapsulated gold nanorod (GNR)-virus-inspired mesoporous silica core-shell nanoparticles (vinyl hybrid silica nanoparticles; VSNP) ([email protected]) were synthesized to achieve synergetic therapy by utilizing selective vascular thrombosis therapy (SVTT) and photothermal therapy (PTT). By integrating the targeted coagulation activity of tTF-EG3287 and the high tumor ablation effect of [email protected], local hyperthermia could induce a high percentage of apoptosis of vascular endothelial cells by using near-infrared light. This provided additional phospholipid sites for tTF-EG3287 and enhanced its procoagulant activity . In addition, the nanoparticles, which had unique topological viral structures, exhibited superior cellular uptake properties leading to significant antitumor efficacy. The antitumor results further demonstrated an interaction between SVTT and PTT, whereas the synergetic therapy (SVTT and PTT) achieved an enhanced effect, which was superior to the respective treatment efficacy of each modality or the additive effect of their individual efficacies. In summary, the synthesized [email protected] exerted synergetic effects and enhanced the antitumor efficiency by avoiding multiple injections and suboptimal administration. These effects simultaneously affected both tumor blood supply and cancer cell proliferation. The data suggested that the integration of SVTT induced by tTF-EG3287 and PTT could provide potential strategies for synergetic tumor therapy.
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http://dx.doi.org/10.1021/acsami.1c11947DOI Listing
September 2021

OsHyPRP06/R3L1 regulates root system development and salt tolerance via apoplastic ROS homeostasis in rice (Oryza sativa L.).

Plant Cell Environ 2021 Sep 7. Epub 2021 Sep 7.

College of Plant Protection, Henan Agricultural University, Zhengzhou, China.

Plant root morphology is constantly reshaped in response to triggers from the soil environment. Such modifications in root system architecture involve changes in the abundance of reactive oxygen species (ROS) in the apoplast and in cell wall (CW) composition. The hybrid proline-rich proteins (HyPRPs) gene family in higher plants is considered important in the regulation of CW structure. However, the functions of HyPRPs remain to be characterized. We therefore analysed the functions of OsR3L1 (Os04g0554500) in rice. qRT-PCR and GUS staining revealed that OsR3L1 is expressed in roots. While the r3l1 mutants had a defective root system with fewer adventitious roots (ARs) and lateral roots (LRs) than the wild type, lines overexpressing OsR3L1 (R3L1-OE) showed more extensive LR formation but with a shorter root length. The expression of OsR3L1 was initiated by the OsMADS25 transcription factor. Moreover, the abundance of OsR3L1 transcripts was increased by NaCl. The R3L1-OE-3 line exhibited enhanced salt tolerance, whereas the r3l1-2 mutant showed greater salt sensitivity. The addition of H O increased the levels of OsR3L1 transcripts. Data are presented indicating that OsR3L1 modulates H O accumulation in the apoplast. We conclude that OsR3L1 regulates salt tolerance through regulation of peroxidases and apoplastic H O metabolism.
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http://dx.doi.org/10.1111/pce.14180DOI Listing
September 2021

Changes over 10 years in the nursing workforce in Guangdong province, China: Three-wave multisite surveys.

J Nurs Manag 2021 Nov 19;29(8):2630-2638. Epub 2021 Aug 19.

School of Nursing, Sun Yat-sen University, Guangzhou, China.

Aims: The study aimed to describe the changes in the nursing workforce in 2008-2018 in Guangdong province, China.

Background: A strong nursing workforce is important in the development of the health care system in China. However, whether the nursing workforce in China has improved is underexplored.

Methods: Three waves of surveys were conducted in hospitals in Guangdong province, China, in 2008, 2014 and 2018.

Findings: The proportion of less experienced nurses and nurses holding a bachelor's degree has increased. The hospital nurse-to-patient ratio did not change significantly. The work environment deteriorated from 2008 to 2014 and improved from 2014 to 2018. Nurse-perceived staffing adequacy and nurses participating in hospital administration were scored lowest. The nurse-physician relations declined from 2008 to 2018. Nurse satisfaction, retention and quality of care improved, while reduced personal accomplishment deteriorated.

Conclusion: The nursing workforce in Guangdong province, China, is young and highly educated. Nurse outcomes and quality of care have made progress from 2008 to 2018. Nurse staffing and burnout remain matters of concern.

Implications For Nursing Management: Strategies addressing nursing workforce issues in China include dealing with the nursing shortage, establishing pathways for nurses' participation in decision-making, increasing nurses' income and welfare, promoting recognition of nurses and improving the quality of care.
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http://dx.doi.org/10.1111/jonm.13438DOI Listing
November 2021

Sono-Fenton Chemistry Converts Phenol and Phenyl Derivatives into Polyphenols for Engineering Surface Coatings.

Angew Chem Int Ed Engl 2021 09 25;60(39):21529-21535. Epub 2021 Aug 25.

Key Laboratory of Colloid and Interface Chemistry of the Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan, Shandong, 250100, China.

We report a sono-Fenton strategy to mediate the supramolecular assembly of metal-phenolic networks (MPNs) as substrate-independent coatings using phenol and phenyl derivatives as building blocks. The assembly process is initiated from the generation of hydroxyl radicals ( OH) using high-frequency ultrasound (412 kHz), while the metal ions synergistically participate in the production of additional OH for hydroxylation/phenolation of phenol and phenyl derivatives via the Fenton reaction and also coordinate with the phenolic compounds for film formation. The coating strategy is applicable to various phenol and phenyl derivatives and different metal ions including Fe , Fe , Cu , and Co . In addition, the sono-Fenton strategy allows real-time control over the assembly process by turning the high-frequency ultrasound on or off. The properties of the building blocks are maintained in the formed films. This work provides an environmentally friendly and controllable method to expand the application of phenolic coatings for surface engineering.
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http://dx.doi.org/10.1002/anie.202108462DOI Listing
September 2021

[Advances in capillary-based immobilized enzyme microreactor based on DNA-directed immobilization].

Se Pu 2020 Oct;38(10):1206-1210

College of Chemistry, Beijing University of Chemical Technology, Beijing 100029, China.

Life processes such as metabolism and energy conversion are catalyzed by biological enzymes. The changes of enzymatic activity in organisms can lead various diseases. Thus, it is imperative to develop novel methods of analyzing enzymatic activities for gaining deeper insights into metabolic processes, disease diagnosis, and drug development. Capillary electrophoresis (CE) has the advantages of high separation efficiency, fast analysis speed, and simple operation; moreover, it requires less sample and can be combined with a variety of detection methods. Therefore, CE has attracted increasing attention for enzyme analysis. Enzyme analysis based on CE mainly includes off-line mode and on-line mode. In the off-line mode, the enzyme and substrate are incubated outside the capillary, and then the product is introduced into the CE for analysis. In the on-line mode, the capillary is not only used as a separation channel, but also as an enzyme reaction site. Therefore, the on-line mode facilitates all steps of enzymatic hydrolysis, separation, and detection within a capillary. In the on-line mode, homogeneous analysis method, electrophoretically mediated microanalysis (EMMA), and heterogeneous analysis method, immobilized enzyme microreactor (IMER), were developed. The on-line enzyme analysis method of IMER combined with capillary electrophoresis (CE-IMER) was developed into a mainstream enzyme analysis method. CE-IMER combines the advantages of immobilized enzyme and CE. By immobilizing the free enzyme in capillary, it can not only significantly improve the stability and reusability of enzyme, but also enables the automatic enzyme analysis at nanoscale. This can significantly reduce the cost of enzyme analysis. Although, there are numerous methods to prepare new IMER for enzyme analysis by CE, preparing CE-IMER with good performance, reusability, large enzyme loading, and high degree of automation is the focus of research in this field. DNA-directed immobilization (DDI) makes use of the complementary base pairs (A-T, C-G) of DNA molecules to specifically immobilize biomacromolecules under mild physiological conditions. The enzyme can be immobilized on the carrier surface by DDI and the short double helix DNA molecules possess strong mechanical strength and physicochemical stability. This can form an enzyme microarray, reduce the resistance of mass transfer, improve the contact between enzyme and substrate, and promote the enzymatic analysis process. Compared with the traditional immobilization methods of adsorption, crosslinking, encapsulation, and covalent bonding, DDI can be operated under mild physiological conditions. Further, this can significantly reduce the influence of the immobilization process on the activity, conformation, and stability of the enzyme. Meanwhile, the reversible immobilization process of DDI can regenerate the surface of the carrier, thereby significantly reducing the economic and time cost of IMER preparation. Therefore, DDI is an ideal method to prepare IMER. In this article, the preliminary research and progress of our research group in the field of IMER preparation by DDI technology are presented. At present, the research on the preparation of novel IMER based on DNA nanotechnology, such as DDI, is in the initial stage and there is much scope for development and research. Based on the previous studies, we can focus on the following aspects: (1) building a more efficient catalytic IMER cascade reaction system by immobilizing target enzymes in specific regions of the capillary based on DDI; (2) aiming at the problems existing in the preparation of IMER, such as stability, enzymatic activity, and enzyme immobilization capacity, while taking advantages of DNA structure and nanomaterials to prepare novel IMERs to promote the wide application of CE-IMER in enzyme analysis.
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http://dx.doi.org/10.3724/SP.J.1123.2020.05035DOI Listing
October 2020

Nursing home staff perceptions of challenges and coping strategies during COVID-19 pandemic in China.

Geriatr Nurs 2021 Jul-Aug;42(4):887-893. Epub 2021 May 5.

Xiangya school of nursing, Central South University, No.172, tongzipo Road, Yuelu District, Changsha, Hunan province 410013, China. Electronic address:

Background: First-line nursing staff are responsible for protecting residents, the most vulnerable population, from COVID-19 infections. They are at a high risk of being infected with COVID-19 and experience high levels of psychological distress.

Aims: To explore the challenges and coping strategies perceived by nursing staff during the COVID-19 pandemic in China.

Methods: In April,2020, we conducted a qualitative study using in-depth semi-structured interviews with nursing staff. Participants were selected from seven nursing homes in three cities in Hunan Province, China.

Results: A total of 21 nursing staff participated in the study, including seven nurse managers, seven registered nurses and seven nursing assistants. Three main themes were identified. Different groups encountered different sources of stress and adopted various coping strategies to fulfil their responsibilities.

Conclusions: Nursing home staff were underprepared for dealing with COVID-19-related challenges. Educational programs to improve the ability to deal with COVID-19 prevention and control are needed.
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http://dx.doi.org/10.1016/j.gerinurse.2021.04.024DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8098060PMC
August 2021

Bilin-dependent regulation of chlorophyll biosynthesis by GUN4.

Proc Natl Acad Sci U S A 2021 05;118(20)

State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, 430070 Wuhan, China;

Biosyntheses of chlorophyll and heme in oxygenic phototrophs share a common trunk pathway that diverges with insertion of magnesium or iron into the last common intermediate, protoporphyrin IX. Since both tetrapyrroles are pro-oxidants, it is essential that their metabolism is tightly regulated. Here, we establish that heme-derived linear tetrapyrroles (bilins) function to stimulate the enzymatic activity of magnesium chelatase (MgCh) via their interaction with GENOMES UNCOUPLED 4 (GUN4) in the model green alga A key tetrapyrrole-binding component of MgCh found in all oxygenic photosynthetic species, GUN4, also stabilizes the bilin-dependent accumulation of protoporphyrin IX-binding CHLH1 subunit of MgCh in light-grown cells by preventing its photooxidative inactivation. Exogenous application of biliverdin IXα reverses the loss of CHLH1 in the bilin-deficient heme oxygenase () mutant, but not in the mutant. We propose that these dual regulatory roles of GUN4:bilin complexes are responsible for the retention of bilin biosynthesis in all photosynthetic eukaryotes, which sustains chlorophyll biosynthesis in an illuminated oxic environment.
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http://dx.doi.org/10.1073/pnas.2104443118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8158021PMC
May 2021

Potential Mechanisms Mediating the Protective Effects of -Derived Peptides in Mitigating DSS-Induced Colitis.

J Agric Food Chem 2021 May 6;69(19):5536-5546. Epub 2021 May 6.

National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, P. R. China.

Intestinal barrier dysfunction and inflammatory cytokine secretion play crucial roles in inflammatory bowel disease (IBD). Herein, we investigated the protective effects of -derived peptides SDIKHFPF and SDLKHFPF against dextran sulfate sodium-induced colitis. Both peptides alleviated colitis signs, including diarrhea, weight loss, bloody stools, colon shortening, and histopathological changes, while reducing mucus destruction, goblet cell exhaustion, and intestinal permeability. SDIKHFPF and SDLKHFPF protected the barrier function by promoting the expression of tight junction (TJ) zonula occludens-1 and occludin within the colon, as well as attenuating colonic inflammation through myeloperoxidase and pro-inflammatory cytokine suppression. Western blotting indicated that the peptides suppressed myosin light chain kinase (MLCK) and nuclear factor kappa B (NF-κB) levels, inhibiting MLC phosphorylation. SDLKHFPF was more potent than SDIKHFPF. These findings suggest that peptide SDLKHFPF mitigates colitis by regulating TJ protein expression and pro-inflammatory cytokine production via NF-κB/MLCK/p-MLC signaling, improving the barrier function.
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http://dx.doi.org/10.1021/acs.jafc.1c01908DOI Listing
May 2021

Gene Transcript Alterations in the Spinal Cord, Anterior Cingulate Cortex, and Amygdala in Mice Following Peripheral Nerve Injury.

Front Cell Dev Biol 2021 7;9:634810. Epub 2021 Apr 7.

Department of Anatomy, College of Basic Medicine, Zhengzhou University, Zhengzhou, China.

Chronic neuropathic pain caused by nerve damage is a most common clinical symptom, often accompanied by anxiety- and depression-like symptoms. Current treatments are very limited at least in part due to incompletely understanding mechanisms underlying this disorder. Changes in gene expression in the dorsal root ganglion (DRG) have been acknowledged to implicate in neuropathic pain genesis, but how peripheral nerve injury alters the gene expression in other pain-associated regions remains elusive. The present study carried out strand-specific next-generation RNA sequencing with a higher sequencing depth and observed the changes in whole transcriptomes in the spinal cord (SC), anterior cingulate cortex (ACC), and amygdala (AMY) following unilateral fourth lumbar spinal nerve ligation (SNL). In addition to providing novel transcriptome profiles of long non-coding RNAs (lncRNAs) and mRNAs, we identified pain- and emotion-related differentially expressed genes (DEGs) and revealed that numbers of these DEGs displayed a high correlation to neuroinflammation and apoptosis. Consistently, functional analyses showed that the most significant enriched biological processes of the upregulated mRNAs were involved in the immune system process, apoptotic process, defense response, inflammation response, and sensory perception of pain across three regions. Moreover, the comparisons of pain-, anxiety-, and depression-related DEGs among three regions present a particular molecular map among the spinal cord and supraspinal structures and indicate the region-dependent and region-independent alterations of gene expression after nerve injury. Our study provides a resource for gene transcript expression patterns in three distinct pain-related regions after peripheral nerve injury. Our findings suggest that neuroinflammation and apoptosis are important pathogenic mechanisms underlying neuropathic pain and that some DEGs might be promising therapeutic targets.
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http://dx.doi.org/10.3389/fcell.2021.634810DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8059771PMC
April 2021

Nrf2/HO-1 Axis Regulates the Angiogenesis of Gastric Cancer via Targeting VEGF.

Cancer Manag Res 2021 12;13:3155-3169. Epub 2021 Apr 12.

Department of Pathology, Ningxia Medical University, Yinchuan City, Ningxia Province, 750004, People's Republic of China.

Purpose: Gastric cancer (GC) is one of the most fatal digestive tumors worldwide. Abnormal activation or accumulation of the nuclear factor-erythroid 2-related factor 2/heme oxygenase 1 (Nrf2/HO-1) axis is a malignant event in numerous solid tumors. However, its involvement in angiogenesis of GC remains unknown. This study investigated the role of the Nrf2/HO-1 axis in angiogenesis of GC.

Methods: The expression of Nrf2, HO-1, and vascular endothelial growth factor (VEGF) in BGC-823 cells under hypoxia was analyzed using immunocytochemistry, immunofluorescence, Western blotting, and quantitative polymerase chain reaction. The effects of brusatol (Nrf2 inhibitor) and tert-butylhydroquinone (Nrf2 inducer) on these factors and angiogenesis were examined using immunofluorescence, Western blotting, quantitative polymerase chain reaction, and tube formation assay. Moreover, immunohistochemistry and Western blotting were used to determine these factors and microvessel density in tumor and normal tissues of tumor-bearing and tumor-free mice, respectively. Immunohistochemistry and Western blotting were employed to examine these factors and microvessel density in human paracancerous tissues, well-differentiated GC, and poorly differentiated GC. The correlations between Nrf2, HO-1, and VEGF gene expression in 375 patients with GC from The Cancer Genome Atlas cohort were analyzed.

Results: The expression of Nrf2, HO-1, and VEGF was increased in hypoxic BGC-823 cells (<0.05). Although brusatol decreased their expression and angiogenesis (<0.05), tert-butylhydroquinone had the opposite effect (<0.05). Moreover, the expression of Nrf2, HO-1, and VEGF, and microvessel density in tumor tissues was higher than that recorded in normal tissues of nude mice (<0.05). Similarly, these parameters were low in paracancerous tissues, but high in GC tissues (<0.05). Also, they were weak in well-differentiated GC, but strong in poorly differentiated GC (<0.05). In addition, there was a significant correlation between Nrf2, HO-1, and VEGF (<0.05).

Conclusion: The Nrf2/HO-1 axis may regulate the angiogenesis of GC via targeting VEGF. These findings provide a promising biomarker and potential treatment target for GC.
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http://dx.doi.org/10.2147/CMAR.S292461DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8055645PMC
April 2021

Flavor Changes of Singer under Different Processing Conditions by Using HS-GC-IMS.

Foods 2021 Mar 4;10(3). Epub 2021 Mar 4.

National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China.

Headspace-gas chromatography-ion mobility spectrometry (HS-GC-IMS) was used to analyze the changes to volatile compounds in fried Singer under different heating temperatures and times. A total of 40 signals that corresponded to 24 compounds were identified through this approach. Differences in volatile compounds of samples were shown in topographic plots and fingerprints. The heating temperatures were more important than the heating times in affecting the volatile compounds. Moreover, changes to the main volatile compounds in under different processing conditions were based on the thermal decomposition and a series of chemical reactions of C8 compounds. Principal component analysis (PCA) results showed that samples under different processing conditions could be effectively distinguished. Hence, the combination of HS-GC-IMS and PCA can identify and classify the volatile compounds of quickly and sensitively. This study provides a new supplementary analytical method for the rapid identification of T. matsutake. The above results can provide a theoretical basis for the quality control and change mechanism of flavor in the processing of traditional edible fungi products.
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http://dx.doi.org/10.3390/foods10030531DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8000925PMC
March 2021

Effectiveness of Oral Nutritional Supplements on Older People with Anorexia: A Systematic Review and Meta-Analysis of Randomized Controlled Trials.

Nutrients 2021 Mar 3;13(3). Epub 2021 Mar 3.

Xiangya School of Nursing, Central South University, Changsha 410013, China.

Background: Nutrition plays an important role in maintaining the overall health of older people. Inadequate intake may lead to impaired body function, higher morbidity, and mortality. Oral nutritional supplements (ONS) showed positive effect on the nutritional status of the elderly; however, systematic evidence is currently lacking on the effect of ONS on the elderly with anorexia.

Aims: The current systematic review and meta-analysis included randomized controlled trial (RCT) articles to investigate the effectiveness of ONS on the main aspects of anorexia of aging (AA).

Methods: By using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) method, researchers independently searched PubMed/MEDLINE, EMBASE, CINAHL, PsycINFO, the Cochrane Library, China National Knowledge Infrastructure (CNKI) and other gray literature resources for publications that met the inclusion criteria by October 2020. The Cochrane Risk of Bias Tools were used for quality assessment. The inverse-variance method was used for the fixed model (FM) while the DerSimonian-Laird method was used for the random model (RM). Respective 95% confidence intervals (95% CIs), mean difference (MD) or standardized mean difference (SMD) was used for indices in terms of effect size (ES).

Results: 2497 records were found through the systematic search, while 17 RCTs ( = 1204) were included, with a mean age of 81.9 years (range: 74-87 years). Supplementation occurred in the morning, mid-day, and evening, while the times varied from one to three times a day. The results of meta-analysis showed that, generally, ONS had a positive effect on the overall appetite, MD = 0.18, 95% CI (0.03, 0.33), = 0.02, and consumption, MD = 1.43, 95% CI (0.01, 2.86), = 0.05; but not significant in terms of other aspects of appetite: hunger, = 0.73; fullness, = 0.60; desire to eat, = 0.80; preoccupation, = 0.15. Additionally, it showed an increase in the overall energy intake, SMD = 0.46, 95% CI (0.29, 0.63), < 0.001, in protein intake, SMD = 0.59, 95% CI (0.16, 1.02), = 0.007, and in fat intake, MD = 3.47, 95% CI (1.98, 4.97), < 0.001, while no positive effect was found on carbohydrates intake, = 0.06. Significance differences were also found in the body weight, SMD = 0.53, 95% CI (0.41, 0.65), < 0.001, and body mass index (BMI), MD = 0.53, 95% CI (0.12, 0.95), = 0.01. Moreover, subgroup analyses were conducted according to the nutrient density with no positive results showed except for the low-density ONS on overall energy intake.

Conclusions: The results of the present study indicated that ONS had beneficial effects on overall appetite, energy intake, body weight and BMI.
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http://dx.doi.org/10.3390/nu13030835DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8001033PMC
March 2021

Low-level pyruvate inhibits early embryonic development and maternal mRNA clearance in mice.

Theriogenology 2021 May 3;166:104-111. Epub 2021 Mar 3.

State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning, Guangxi, 530004, China; College of Animal Science & Technology, Guangxi University, Nanning, Guangxi, 530004, China. Electronic address:

Energy homeostasis and accomplishment of maternal-to-zygotic transition (MZT), which involves the timed processes of maternal mRNA clearance and zygotic genome activation (ZGA), are essential for mammalian embryogenesis. However, how energy substrates regulate maternal mRNA clearance and the underlying mechanisms have not yet been fully elucidated. Here, we found that mouse embryos were arrested at the 2-cell stage when the pyruvate level was reduced to one-fifth of the control level. Moreover, we observed that the mitochondrial contents and ROS levels were reduced. Interestingly, some maternal mRNA, including transcripts involved in the maternal factor-mediated mRNA decay (M-decay) pathway, was vastly degraded from 1-cell to 2-/4-cell embryos when cultured with control pyruvate levels, but the clearance of these transcripts was hindered when the pyruvate level was reduced. In contrast, some transcripts involved in the zygotic factor-mediated mRNA decay (Z-decay) pathway were vastly downregulated by the reduction in pyruvate. This effect was possibly due to a reduction in global transcription, as the embryos cultured with low-level pyruvate had lower transcription activity than embryos cultured with control pyruvate level. In summary, our findings demonstrate that low-level pyruvate inhibits maternal mRNA clearance, possibly by disrupting the M- and Z-decay pathways, extending our current understanding of the energy requirements of embryogenesis.
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http://dx.doi.org/10.1016/j.theriogenology.2021.02.022DOI Listing
May 2021

Potential mechanisms underlying the protective effects of Tricholoma matsutake singer peptides against LPS-induced inflammation in RAW264.7 macrophages.

Food Chem 2021 Aug 3;353:129452. Epub 2021 Mar 3.

National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China. Electronic address:

This study aimed to investigate the protective effects of a < 3 kDa Tricholoma matsutake Singer peptide (TMWP) on lipopolysaccharide (LPS)-induced inflammation in RAW264.7 macrophages. The results showed that TMWP significantly upregulated superoxide dismutase (SOD) activity and reduced reactive oxygen species (ROS) generation in RAW264.7 macrophages. Western blotting and immunofluorescence analysis indicated that TMWP inhibited the activation of the mitogen-activated protein kinase (MAPK) and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathways, thereby reducing the secretion of IL-1β and IL-6 and the expression of TNF-α, COX-2, and iNOS. Additionally, TMWP improved mitochondrial respiration in LPS-stressed macrophages, counteracting the harmful effects of LPS treatment on mitochondrial function. Three peptides (SDIKHFPF, SDLKHFPF, and WFNNAGP) with the highest predicted scores for potential anti-inflammatory activity were identified using nano-HPLC-MS/MS. These data indicated that T. matsutake peptides could be an attractive natural ingredient for developing novel functional foods.
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http://dx.doi.org/10.1016/j.foodchem.2021.129452DOI Listing
August 2021

Association of changes in nursing work environment, non-professional tasks, and nursing care left undone with nurse job outcomes and quality of care: A panel study.

Int J Nurs Stud 2021 Mar 29;115:103860. Epub 2020 Dec 29.

School of Nursing, Sun Yat-sen University, Guangzhou, China. Electronic address:

Background: Strengthening quality of care without compromising nurse job outcomes by building a safer health care system is a common concern worldwide including in China. Most of the current evidence comes from cross-sectional studies conducted in western countries, which limits inferences of causality and generalization.

Objective: The objectives of this longitudinal study were to compare changes in quality of care, nurse job outcomes, nursing work environment, non-professional tasks, and nursing care left undone in acute hospitals in China between 2014 and 2018. Secondly, we wanted to determine the association of changes in nursing work environment, non-professional tasks, and nursing care left undone with nurse job outcomes and quality of care.

Design, Settings, And Participants: A prospective two-stage panel study conducted in 108 adult medical and surgical units from 23 hospitals in Guangdong province, China in 2014 and repeated in 2018.

Methods: Work environment was measured by the Practice Environment Scale of the Nursing Work Index. Non-professional tasks were measured with a seven-item scale surveying the performance of and time spent on non-professional tasks. Nursing care left undone was measured by 12 items addressing necessary nursing activities. Nurse job outcomes included burnout, dissatisfaction, and retention. Quality of care was measured by four items indicating overall quality of care as assessed by nurses (three items) and patients (one item). Generalized estimating equations with linear regression were employed to analyze data.

Results: In 2018, compared with 2014, the nursing work environment had improved, and non-professional workloads had decreased minimally. The average number of the 12 nursing care tasks left undone had increased to 6.5 from 5.6 in 2014. Fewer nurses reported job dissatisfaction or intention to leave. Quality of care was improved slightly as assessed by nurses and patients. As for the changes of hospital organizational factors on quality of care, a better nursing work environment was related to better nurse job outcomes and quality of care. More non-professional tasks were related to higher levels of nurse job burnout. Less nursing care left undone was associated with better nurse-assessed quality of care. Units with more nurses experiencing job burnout and dissatisfaction were likely to have poorer nurse-assessed quality of care.

Conclusions: Improving nursing work environment and supporting nurses to engage in professional and direct patient care as opposed to non-professional work may be beneficial to nurse job outcomes and promote quality of care.
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http://dx.doi.org/10.1016/j.ijnurstu.2020.103860DOI Listing
March 2021

Inhibition of Nrf2/HO-1 signaling pathway by Dextran Sulfate suppresses angiogenesis of Gastric Cancer.

J Cancer 2021 1;12(4):1042-1060. Epub 2021 Jan 1.

Department of Pathology, Ningxia Medical University, Yinchuan, Ningxia 750004, China.

To investigate the role of Nrf2/HO-1 signaling pathway in angiogenesis and whether dextran sulfate (DS) could suppress angiogenesis by inhibiting Nrf2/HO-1 signaling pathway in gastric cancer. Western blot analyzed the expression of Nrf2 in gastric cell lines. Tube formation assay observed the effect of gradient concentration DS on the angiogenic potential of HGC-27 cells. Immunofluorescence,western blot and qPCR analyzed the effects of DS on the expression of Nrf2, HO-1 and VEGF under gradient hypoxia time. Immunofluorescence,western blot,qPCR and tube formation assay analyzed the effects of up-regulating or down-regulating Nrf2/HO-1 signaling pathway on VEGF expression and angiogenic potential in HGC-27 cells. Construct nude mouse intraperitoneal implantation metastasis model. Immunohistochemistry and western blot analyzed the effects of DS on the expression of Nrf2, HO-1, VEGF and MVD in nude mice. Immunohistochemistry detected the expression of Nrf2, HO-1, VEGF and MVD in human paracancerous tissue and gastric cancer tissues with different degrees of differentiation. The expression of Nrf2 increased most significantly in HGC-27 cell line. DS reduced the angiogenic potential and the expression of Nrf2, HO-1 and VEGF in HGC-27 cells. Down-regulation of Nrf2/HO-1 signaling pathway decreased VEGF expression and angiogenic potential in HGC-27 cells. Up-regulation of Nrf2/HO-1 signaling pathway increased VEGF expression and angiogenic potential in HGC-27 cells. DS reduced the expression of Nrf2, HO-1, VEGF and MVD in nude mice. Nrf2, HO-1, VEGF and MVD showed low expression in paracancerous tissue but high expression in gastric cancer tissues. They were weak, moderate and strong in well, moderately and poorly differentiated gastric cancer tissues, respectively. Nrf2/HO-1 signaling pathway may positively regulate gastric cancer angiogenesis and DS may suppress the angiogenesis by inhibiting Nrf2/HO-1 signaling pathway in gastric cancer.
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http://dx.doi.org/10.7150/jca.50605DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7797653PMC
January 2021

Hepatitis B virus rigs the cellular metabolome to avoid innate immune recognition.

Nat Commun 2021 01 4;12(1):98. Epub 2021 Jan 4.

State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, 430072, China.

Glucose metabolism and innate immunity evolved side-by-side. It is unclear if and how the two systems interact with each other during hepatitis B virus (HBV) infections and, if so, which mechanisms are involved. Here, we report that HBV activates glycolysis to impede retinoic acid-inducible gene I (RIG-I)-induced interferon production. We demonstrate that HBV sequesters MAVS from RIG-I by forming a ternary complex including hexokinase (HK). Using a series of pharmacological and genetic approaches, we provide in vitro and in vivo evidence indicating that HBV suppresses RLR signaling via lactate dehydrogenase-A-dependent lactate production. Lactate directly binds MAVS preventing its aggregation and mitochondrial localization during HBV infection. Therefore, we show that HK2 and glycolysis-derived lactate have important functions in the immune escape of HBV and that energy metabolism regulates innate immunity during HBV infection.
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http://dx.doi.org/10.1038/s41467-020-20316-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7782485PMC
January 2021

Exposure to organophosphate ester flame retardants and plasticizers during pregnancy: Thyroid endocrine disruption and mediation role of oxidative stress.

Environ Int 2021 01 24;146:106215. Epub 2020 Oct 24.

Ministry of Education Key Laboratory of Pollution Processes and Environmental Criteria, College of Environmental Science and Engineering, Nankai University, 38 Tongyan Road, Jinnan District, Tianjin, China. Electronic address:

Organophosphate esters (OPEs) are widely used as flame retardants and plasticizers in consumer and industrial products. Human exposure to OPEs raises concerns due to their endocrine disruptive potentials. Till now, the effects of OPEs on thyroid hormones (THs) and the mediating role of oxidative stress in pregnant women have not been studied. In this study, prenatal urinary concentrations of OPE metabolites (mOPEs), levels of free triiodothyronine (FT3), free thyroxine (FT4), thyroid-stimulating hormone (TSH), and oxidative stress levels of 8-hydroxy-2-deoxy guanosine (8-OHdG) and malondialdehyde (MDA) were measured in pregnant women (n = 360) from a coastal urbanized region and moderate socioeconomic status. Neonatal TSH in heel blood was also measured in newborns (n = 309). Dibutyl phosphate (DBP) and diphenyl phosphate (DPHP) were extensively detected with a median creatinine-adjusted level of 0.19 μg/g and 0.66 μg/g, respectively, and the median of ∑mOPEs was 1.82 μg/g. DBP and DPHP were included in the analysis. The concentrations of DBP and DPHP were positively associated with either maternal or neonatal TSH levels, while not for maternal FT3 and FT4 levels. Positive associations for maternal and neonatal TSH were particularly observed in girls as stratified by newborn sex suggesting a sex-selective difference. Furthermore, 8-OHdG, the biomarker of DNA damage, was found to be a major mediator (>60%) for the association between neonatal TSH and DPHP, suggesting that DNA damage is involved in fetal thyroid function disruption. On the other hand, MDA showed a partially suppressing effect (<40%) for the associations between mOPEs and neonatal TSH, which needs further clarification. For maternal TSH, both 8-OHdG and MDA showed moderate mediating effects while the direct effects of mOPEs on maternal TSH also contributed. These results suggest thyroid disrupting effects of OPE exposure on mothers and fetuses during pregnancy and the potential influence mediated by the oxidative stresses of DNA damage and lipid peroxidation.
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http://dx.doi.org/10.1016/j.envint.2020.106215DOI Listing
January 2021

Differentially Expressed Genes in the Brain of Aging Mice With Cognitive Alteration and Depression- and Anxiety-Like Behaviors.

Front Cell Dev Biol 2020 28;8:814. Epub 2020 Aug 28.

Department of Anesthesiology, Pain and Perioperative Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Despite the great increase in human lifespan with improved medical care, the physiological and pathological changes such as memory and cognitive disorders and associated anxiety and depression are major concern with aging. Molecular mechanisms underlying these changes are little known. The present study examined the differentially expressed genes (DEGs) and the genes with differentially expressed isoforms in three brain regions, anterior cingulate cortex (ACC), amygdala and hippocampus, throughout the lifespan of mice. Compared to 2-month old mice, both 12- and 24-month old mice displayed memory and cognitive impairments in the Morris water maze, Y-maze, and novel object recognition tests and depression- and anxiety-like behaviors in the tail suspension, forced swimming, open field, and elevated plus maze tests. RNA sequencing analysis identified 634 and 1078 DEGs in ACC, 453 and 1015 DEGs in the amygdala and 884 and 1054 DEGs in hippocampus in the 12- and 24-month old mice, respectively. Similarly, many genes with differentially expressed isoforms were also identified in these three brain regions in the 12- and 24-month old mice. Further functional analysis revealed that many DEGs and the genes with differentially expressed isoforms in the ACC and amygdala were mapped to depression- and anxiety-related genes, respectively and that a lot of DEGs and the genes with differentially expressed isoforms in hippocampus were mapped to cognitive dysfunction-related genes from both 12- and 24-month old mice. All of these mapped DEGs and the genes with differentially expressed isoforms were closely related to neuroinflammation. Our findings indicate that these neuroinflammation-related DEGs and the genes with differentially expressed isoforms are likely new targets in the management of memory/cognitive impairment and emotional disorders during the aging.
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http://dx.doi.org/10.3389/fcell.2020.00814DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7493670PMC
August 2020

Electrically controllable cargo delivery with dextran-rich droplets.

J Colloid Interface Sci 2021 Jan 11;582(Pt A):102-111. Epub 2020 Aug 11.

Department of Mechanical and Mechatronics Engineering, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada. Electronic address:

The controllable delivery of cargo is of great importance in many areas, ranging from medicine and materials science to food and cosmetic industries. To fulfil the requirements in different areas, the development of new methods for cargo delivery in a controllable manner is always essential. A novel technique of cargo delivery controlled by electric pulse was developed in this paper. In an aqueous two-phase system, the dextran-rich droplets were fabricated as droplet carriers in a continuous polyethylene glycol-rich phase. The loading and releasing of model cargos (polystyrene particles) across the surface of the droplet carriers under electric pulses were demonstrated in microfluidic chips. By controlling the amplitude of the applied electric pulses, the cargos with designed sizes were sorted and loaded into the droplet carriers; hence, the targeted delivery of cargos by size can be achieved. The exchange of cargos between droplet carriers under reversed electric pulses was also investigated, and the results indicated the flexibility of this method in cargo delivery. Moreover, possible application of this method to biological cargos was demonstrated by controlling the loading and releasing of yeast cells under electric pulses. With the advantages of easy operation and fast response, this approach provides a novel route for controllable cargo delivery with droplets.
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http://dx.doi.org/10.1016/j.jcis.2020.08.033DOI Listing
January 2021

Oriented immobilization of enzyme-DNA conjugates on magnetic Janus particles for constructing a multicompartment multienzyme system with high activity and stability.

J Mater Chem B 2020 09;8(36):8467-8475

Beijing Key Laboratory of Environmentally Harmful Chemical Analysis, College of Chemistry, Beijing University of Chemical Technology, Beijing 100029, P. R. China.

Various organelles (e.g., mitochondria and chloroplasts) have a multicompartment structure, providing superior function of material transformation, selective segregation and energy conversion. Enlightened by the elegant evolution of nature, intended isolation of the biochemical process by cooperative multicompartments in cells has become an appealing blueprint to construct bioreactors. In this study, we develop a "soft separation" way to establish a delicate multicompartment multienzyme system (MMS) with polyphenol-encapsulated enzyme-DNA conjugates, which are anchored on magnetic Janus particles, providing a biomimetic catalysis network with the model cascade reactions in confinement. The well-designed MMS exhibits preferable bioactivity benefitting from the dependable DNA bridges and the oriented immobilization of enzymes, while the polyphenol shell further protects the anchored enzymes from exterior attacks, such as heat and enzymatic degradation. Moreover, by applying the MMS as nanomotors, the asymmetrical distribution of enzymes on Janus particles is found to improve mutual elevation between the self-driven locomotion and enzyme-mediated reactions, delivering enhanced dispersal ability and bioactivity. Owing to the excellent enzymatic activity, promoted stability and satisfying biocompatibility, the assembled MMS is proved to be promising for the in vitro and intracellular sensing of glucose, showing significant potential for biochemical analysis applications.
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http://dx.doi.org/10.1039/d0tb01439gDOI Listing
September 2020

Microbial community responses to agricultural biomass addition in aerated constructed wetlands treating low carbon wastewater.

J Environ Manage 2020 Sep 9;270:110912. Epub 2020 Jun 9.

College of Resources and Environment, Northwest A&F University, Yangling, Shaanxi, 712100, PR China; Department of Bioscience, Aarhus University, Aarhus, 8000C, Denmark. Electronic address:

Using agricultural biomasses as solid carbon substrates in constructed wetlands (CWs) could be an effective way to achieve sustainable nitrogen removal for carbon-limited wastewater treatments. This study investigated the response of bacteria community in CWs to the addition of agricultural biomasses (wheat straw, walnut shell and apricot pit). Results indicated that the addition of different agricultural biomasses had distinct influence on bacterial communities in CWs. Both wheat straw and walnut shell increased the diversity of microbial communities and optimized the structure of microorganisms. The effect of apricot pit on the richness and evenness of microbial communities was not significant, but the composition of microorganisms was significantly affected at the phylum level, especially the relative abundance of phylum Saccharibacteria. Moreover, the addition of agricultural biomasses in CWs acclimatized more functional bacteria including nitrifier and denitrifier, which were proved to be positively correlated with the high-rate denitrification performance. The obtained results would be beneficial to understand the underlying microbial mechanism of nitrogen removal in CWs with agricultural biomass and provide some guidance on the practical application of CWs.
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http://dx.doi.org/10.1016/j.jenvman.2020.110912DOI Listing
September 2020

Pharmacological Targeting of Vacuolar H-ATPase via Subunit V1G Combats Multidrug-Resistant Cancer.

Cell Chem Biol 2020 11 9;27(11):1359-1370.e8. Epub 2020 Jul 9.

State Key Laboratory of Cellular Stress Biology, Innovation Center for Cell Signaling Network, School of Life Sciences, Xiamen University, Xiamen, Fujian 361102, China; State-province Joint Engineering Laboratory of Targeted Drugs from Natural Products, Xiamen University, Xiamen, Fujian 361102, China. Electronic address:

Multidrug resistance (MDR) in cancer remains a major challenge for the success of chemotherapy. Natural products have been a rich source for the discovery of drugs against MDR cancers. Here, we applied high-throughput cytotoxicity screening of an in-house natural product library against MDR SGC7901/VCR cells and identified that the cyclodepsipeptide verucopeptin demonstrated notable antitumor potency. Cytological profiling combined with click chemistry-based proteomics revealed that ATP6V1G directly interacted with verucopeptin. ATP6V1G, a subunit of the vacuolar H-ATPase (v-ATPase) that has not been previously targeted, was essential for SGC7901/VCR cell growth. Verucopeptin exhibited strong inhibition of both v-ATPase activity and mTORC1 signaling, leading to substantial pharmacological efficacy against SGC7901/VCR cell proliferation and tumor growth in vivo. Our results demonstrate that targeting v-ATPase via its V1G subunit constitutes a unique approach for modulating v-ATPase and mTORC1 signaling with great potential for the development of therapeutics against MDR cancers.
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http://dx.doi.org/10.1016/j.chembiol.2020.06.011DOI Listing
November 2020
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