Publications by authors named "Mengmeng Liu"

174 Publications

Nell-1 attenuates lipopolysaccharide-induced inflammation in human dental pulp cells.

J Mol Histol 2021 Apr 27. Epub 2021 Apr 27.

School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, No.44-1 Wenhua Road West, Jinan, 250012, Shandong, China.

Nel-like molecule type 1 (Nell-1) is a secreted protein that plays an important role in osteoinduction in multiple animal models. A previous study has suggested the anti-inflammatory effect of Nell-1 on bone inflammation inhibition. However, its role in pulpitis has not been investigated. The present study aims to explore the effect of human recombinant Nell-1 (Nell-1) on rat pulp inflammation response, and its effect on lipopolysaccharide-induced inflammation in human dental pulp cells and its related intracellular signaling pathways. 30 Wistar rats with healthy non-carious maxillary first molars were chosen, Nell-1 was absorbed onto a sterile collagen sponge and capped onto exposed pulps. The expression of IL-6 and IL-8 were detected by immunohistochemical staining. Human dental pulp cells (hDPCs) were isolated from healthy extracted premolars and third molars. hDPCs were co-cultured with Escherichia coli lipopolysaccharide (LPS), Nell-1 protein, and mitogen-activated protein kinase (MAPK) inhibitors. The expression of pro-inflammatory cytokines and chemokines, such as IL-6 and IL-8, was examined via quantitative real-time PCR and enzyme-linked immunosorbent assay. The results showed that Nell-1 inhibited the inflammatory response of rat pulp. LPS treatment contributed to the expression of inflammatory factors in hDPCs, whereas Nell-1 obviously suppressed the LPS-induced inflammation. p38 MAPK and extracellular signal-regulated kinase (ERK) MAPK inhibitors attenuated the anti-inflammatory effect of hrNell-1, whereas the c-Jun N-terminal kinases (JNK) MAPK inhibitor exerted minimal effect. Therefore Nell-1 could inhibit LPS-induced inflammation in human dental pulp cells, and this effect may be mediated by p38 and ERK MAPK signaling pathways, but not JNK MAPK signaling pathway.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10735-021-09976-yDOI Listing
April 2021

Acute effect of particulate matter pollution on hospital admissions for stroke among patients with type 2 diabetes in Beijing, China, from 2014 to 2018.

Ecotoxicol Environ Saf 2021 Jul 8;217:112201. Epub 2021 Apr 8.

School of Public Health, Capital Medical University, Beijing 100069, China; Beijing Municipal Key Laboratory of Clinical Epidemiology, Beijing 100069, China. Electronic address:

Background: The health effect of particulate matter pollution on stroke has been widely examined; however, the effect among patients with comorbid type 2 diabetes (T2D) in developing countries has remained largely unknown.

Methods: A time-series study was conducted to investigate the short-term effect of fine particulate matter (PM) and inhalable particulate matter (PM) on hospital admissions for stroke among patients with T2D in Beijing, China, from 2014 to 2018. An over-dispersed Poisson generalized additive model was employed to adjust for important covariates, such as weather conditions and long-term and seasonal trends.

Results: A total of 159,298 hospital admissions for stroke comorbid with T2D were reported. Approximately linear exposure-response curves were observed for PM and PM in relation to stroke admissions among T2D patients. A 10 μg/m increase in the four-day moving average of PM and PM was associated with 0.14% (95% confidence interval [CI]: 0.05-0.23%) and 0.14% (95% CI: 0.06-0.22%) incremental increases in stroke admissions among T2D patients, respectively. A 10 μg/m increase in PM in the two-day moving average corresponded to a 0.72% (95% CI: 0.02-1.42%) incremental increase in hemorrhagic stroke, and a 10 μg/m increase in PM in the four-day moving average corresponded to a 0.14% (95% CI: 0.06-0.22%) incremental increase in ischemic stroke.

Conclusions: High particulate matter might be a risk factor for stroke among patients with T2D. PM and PM have a linear exposure-response relationship with stroke among T2D patients. The study provided evidence of the risk of stroke due to particulate matter pollution among patients with comorbid T2D.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ecoenv.2021.112201DOI Listing
July 2021

In-suit Growth of Cu4SnS4Nanoplates on Reduced Graphene Oxide (rGO) with Ligand Exchange Exhibiting Enhanced Photodegradation Property.

Nanotechnology 2021 Mar 29. Epub 2021 Mar 29.

State Key Laboratory of Material-Oriented Chemical Engineering, College of Chemical Engineering, Nanjing Tech University, Nanjing, Jiangsu, CHINA.

In the present study, a novel Cu4SnS4/reduced graphene oxide (CTS/rGO) composite was successfully prepared using a simple one-pot heat-up method. Post-synthetic ligand exchange (LE) and annealing process were performed to further increase the dispersibility and the conductivity of the prepared composite. An unexpected phase transformation from CTS to Cu3SnS4 with an enhanced absorption in the near-infrared (NIR) region were observed after LE. Furthermore, the photodegradation of Rhodamine B (RhB) by the CTS/rGO composite was investigated. The CTS nanoplates with 10 wt.% rGO treated through LE (CTS-10%rGO-LE) exhibited the highest (99.92%) degradation rate of RhB after 90 min of visible-light irradiation, which is approximately 10 and 1.28 times that of the pure CTS and the CTS-10%rGO treated using annealing (CTS-10%rGO-A). The enhancement of the photodegradation activity could be ascribed to the in-suit growth of CTS on rGO and the subsequent LE treatment, which effectively reduced the agglomeration of CTS and increased the electron-transfer ability of the composite materials. The CTS/rGO composite also exhibited high chemical stability of the photodegradation of RhB after four recycles. The electron paramagnetic resonance (EPR) spectra reveal that•OH and h+ are the main active species in the photocatalytic degradation of RhB with CTS-LE and CTS-10%rGO-LE photocatalysts. The in-suit growth of the CTS/rGO composite with a subsequent LE treatment has the potential to serve as an efficient photocatalysts for the degradation of organic pollutants.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1088/1361-6528/abf301DOI Listing
March 2021

Inhibition of fibroblast growth factor-inducible 14 attenuates experimental tubulointerstitial fibrosis and profibrotic factor expression of proximal tubular epithelial cells.

Inflamm Res 2021 May 23;70(5):553-568. Epub 2021 Mar 23.

Department of Dermatology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710004, China.

Background And Aim: As a proinflammatory cytokine, tumor necrosis factor-like weak inducer of apoptosis (TWEAK) participates in the progression of renal fibrosis by binding to its receptor, fibroblast growth factor-inducible 14 (Fn14). However, the effect of Fn14 inhibition on tubular epithelial cell-mediated tubulointerstitial fibrosis remains unclear. This study aimed to elucidate the role of TWEAK/Fn14 interaction in the development of experimental tubulointerstitial fibrosis as well as the protective effect of Fn14 knockdown on proximal tubular epithelial cells.

Methods: A murine model of unilateral ureteral obstruction was constructed in both wild-type and Fn14-deficient BALB/c mice, followed by observation of the tubulointerstitial pathologies.

Results: Fn14 deficiency ameliorated the pathological changes, including inflammatory cell infiltration and cell proliferation, accompanied by reduced production of profibrotic factors and extracellular matrix deposition. In vitro experiments showed that TWEAK dose-dependently enhanced the expression of collagen I, fibronectin, and α-smooth muscle actin in proximal tubular epithelial cells. Interestingly, TWEAK also upregulated the expression levels of Notch1/Jagged1. Fn14 knockdown and Notch1/Jagged1 inhibition also mitigated the effect of TWEAK on these cells.

Conclusions: In conclusion, TWEAK/Fn14 signals contributed to tubulointerstitial fibrosis by acting on proximal tubular epithelial cells. Fn14 inhibition might be a therapeutic strategy for protecting against renal interstitial fibrosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00011-021-01455-0DOI Listing
May 2021

Programming folding cooperativity of the dimeric i-motif with DNA frameworks for sensing small pH variations.

Chem Commun (Camb) 2021 Apr 1;57(26):3247-3250. Epub 2021 Mar 1.

Institute of Molecular Medicine, Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China.

The response sensitivity of a molecular sensor is determined by the folding cooperativity of its responsive module. Using an H-responsive dimeric DNA i-motif as a model, we demonstrate the enhancement of its folding cooperativity through preorganization by a DNA framework, and with it we fabricate robust intracellular pH sensors with high response sensitivity.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d1cc00266jDOI Listing
April 2021

The adsorption efficiency of nitrogen and phosphorus by in-situ remediation of modified sediment composite material.

Water Sci Technol 2021 Feb;83(4):922-933

School of Municipal and Environmental Engineering, Shandong Jianzhu University, Jinan 250000, China E-mail:

Dredged sediment can occupy a large amount of land area, resulting in waste of land resources, and high disposal costs. In response to the problem, this work calcinates and modified the sediment and compounds it with the modified water purification plant sludge, zeolite powder, and bentonite. This is used as a covering material to inhibit the release of nitrogen (N) and phosphorus (P) in the sediment. The results showed that sediment modified composite material covering effectively reduces the release of nitrogen (N) and phosphorus (P) in the sediment, especially the release of P. When the thickness of the covering layer is 3 cm, the reduction rate of total N, NH-N, and total P in the overlying water by the modified composite material of sediment is 61.58, 79.59, and 70.34%, respectively. It can be seen that the covering material has a significant effect on the control of the release of N and P in the sediment. Additionally, the reduction of nutrients in the overlying water can overcome the negative effects of temperature rise in controlling the release of N and P in the sediment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2166/wst.2021.021DOI Listing
February 2021

Ribosomal Protein SA-Positive Neutrophil Elicits Stronger Phagocytosis and Neutrophil Extracellular Trap Formation and Subdues Pro-Inflammatory Cytokine Secretion Against Serotype 2 Infection.

Front Immunol 2020 2;11:585399. Epub 2021 Feb 2.

College of Veterinary Medicine, Jilin University, Changchun, China.

serotype 2 (SS2), an important zoonotic pathogen that causes septicemia, arthritis, and irreversible meningitis in pigs and humans, can be transmitted to humans from pigs. causes huge economic losses to the swine industry and poses a serious threat to public health. Previously, we found that the brain tissues of mice with SS2-induced meningitis showed disrupted structural integrity and significantly enhanced polymorphonuclear neutrophil (PMN) infiltration. We showed that the brain tissues of SS2-infected mice had increased ribosomal protein SA (RPSA)-positive PMN counts. However, the inflammatory responses of RPSA PMNs to SS2 and their effects on the blood-brain barrier (BBB) remain unclear. Therefore, in studying the pathogenesis of SS2-induced meningitis, it is essential that we explore the functions of RPSA PMNs and their effects on the BBB. Herein, using flow cytometry and immunofluorescence microscopy analyses, we found that RPSA expression enhances PMN-induced phagocytosis and PMN-induced formation of neutrophil extracellular traps (NETs), which facilitate further elimination of bacteria. PMN surface expression of RPSA also alleviates local inflammation and tissue injuries by inhibiting secretion of the pro-inflammatory cytokines, TNF-α and IL-6. Moreover, the single-cell BBB model showed that RPSA disrupts BBB integrity by downregulating expression of tight junction-associated membrane proteins on PMNs. Taken together, our data suggest that PMN-surface expression of RPSA is a double-edged sword. RPSA+ PMN owns a stronger ability of bacterial cleaning and weakens inflammatory cytokines release which are useful to anti-infection, but does hurt BBB. Partly, RPSA+ PMN may be extremely useful to control the infection as a therapeutic cellular population, following novel insights into the special PMN population.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fimmu.2020.585399DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7884477PMC
February 2021

Characteristics and Diversity of Endophytic Bacteria in Endangered Chinese Herb Based on Illumina Sequencing.

Pol J Microbiol 2020 Sep 8;69(3):283-291. Epub 2020 Sep 8.

College of Traditional Chinese Medicine, Hebei University, Baoding, China.

is an endangered medicinal plant growing in the coastal ecological environment and plays an important role in coastal ecosystems. The endophytes in the plant have a significant role in promoting plant growth and enhancing plant stress resistance. However, the endophytic bacterial structure associated with halophyte is still not revealed. In this project, the construction and diversity of endophytic bacterial consortium associated with different tissues of were illustrated with high throughput sequencing of the V3-V4 region of the bacterial 16S rRNA. The results resolved that the diversity and richness of endophytic bacteria were significantly higher in root than in leaf and stem. The operational taxonomic units (OTU) analysis demonstrated that the Actinobacteria and Proteobacteria were dominant in all the samples at the phylum level, and were the dominant genera. Our results unraveled that the bacterial communities differed among different tissues of . Endophytic bacterial communities in leaf and stem shared more similarity than that in the root. Furthermore, the difference of bacteria community and structure among different tissues were also detected by principal coordinate analysis. Taken altogether, we can conclude that the bacterial communities of different tissues are unique, which could facilitate understanding the diversity of endophytic bacteria in .
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.33073/pjm-2020-031DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7810123PMC
September 2020

Analysis of synonymous codon usage of transcriptome database in .

PeerJ 2021 4;9:e10450. Epub 2021 Jan 4.

College of Traditional Chinese Medicine, Hebei University, Baoding, China.

Background: is an endangered and important medicinal plant in Asian countries, especially in China. However, there is little knowledge about the codon usage bias for CDSs. In this project, codon usage bias was determined based on the 2,626 predicted CDSs from R. palmatum transcriptome.

Methods: In this study, all codon usage bias parameters and nucleotide compositions were calculated by Python script, Codon W, DNA Star, CUSP of EMBOSS.

Results: The average GC and GC3 content are 46.57% and 46.6%, respectively, the results suggested that there exists a little more AT than GC in the genes, and the codon bias of genes preferred to end with A/T. We concluded that the codon bias in was affect by nucleotide composition, mutation pressure, natural selection, gene expression levels, and the mutation pressure is the prominent factor. In addition, we figured out 28 optimal codons and most of them ended with A or U. The project here can offer important information for further studies on enhancing the gene expression using codon optimization in heterogeneous expression system, predicting the genetic and evolutionary mechanisms in .
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7717/peerj.10450DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7789865PMC
January 2021

Thermal transformation of polar into less-polar ginsenosides through demalonylation and deglycosylation in extracts from ginseng pulp.

Sci Rep 2021 Jan 15;11(1):1513. Epub 2021 Jan 15.

National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083, China.

The present study was conducted to qualitatively and quantitatively elucidate dynamic changes of ginsenosides in ginseng pulp steamed under different temperatures (100 or 120 °C) for different durations (1-6 h) through UPLC-QTOF-MS/MS and HPLC with the aid of as numerous as 18 authentic standards of ginsenosides. Results show that levels of eight polar ginsenosides (i.e., Rg, Re, Rb, Rc, Rb, Rb, F, and Rd) declined but those of 10 less-polar ginsenosides [i.e., Rf, Rg, 20(S)-Rh, 20(R)-Rg, F, 20(S)-Rg, 20(R)-Rg, PPT, Rg, and 20(R)-Rh] elevated with increases of both steaming temperature and duration; the optimum steaming conditions for achieving the highest total ginsenosides were 100 °C for 1 h. Particular, 20(R)-Rg, a representative less-polar ginsenoside with high bioactivity such as potent anti-cancer effect, increased sharply but Re, the most abundant polar ginsenoside in fresh ginseng pulp, decreased dramatically. More importantly, ginsenoside species enhanced from 18 to 42 after steaming, mainly due to transformation of polar into less-polar ginsenosides. Furthermore, four malonyl-ginsenosides were detected in fresh ginseng pulps and ten acetyl-ginsenosides were formed during steaming, demonstrating that demalonylation and acetylation of ginsenosides were the dominant underling mechanisms for transformation of polar into less-polar ginsenosides.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-021-81079-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7810680PMC
January 2021

Caveolae/rafts protect human cerebral microvascular endothelial cells from Streptococcus suis serotype 2 α-enolase-mediated injury.

Vet Microbiol 2021 Mar 6;254:108981. Epub 2021 Jan 6.

College of Veterinary Medicine, Jilin University, Changchun, PR China; College of Animal Science, Yangtze University, Jingzhou, Hubei, 434023, PR China. Electronic address:

Streptococcus suis serotype 2 (SS2) is an important zoonotic pathogen that causes meningitis. The ubiquitously expressed 40S ribosome protein SA (RPSA) is a multifunctional protein involved in the pathogenesis of multiple pathogens, especially those causing meningitis. However, the role of RPSA in SS2-induced meningitis is not clear. In this study, immunofluorescence staining revealed that SS2 infection promoted the intracellular transfer of RPSA to the surface of human cerebral microvascular endothelial cells (HCMECs). Moreover, SS2 infection promoted the accumulation of caveolin 1 (CAV1) and the formation of membrane bulges where RPSA enveloped CAV1 on the cell surface. SS2 infection also caused dynamic changes in the localization of RPSA and CAV1 on the cell surface which could be eliminated by disruption of caveolae/rafts by addition of methyl-β-cyclodextrin (MβCD). Co-immunoprecipitation analysis demonstrated that α-enolase (ENO), a key virulence factor of SS2, interacted with RPSA, and promoted the interaction between RPSA and CAV1. Immunofluorescence staining, western blotting and flow cytometry analyses showed that damaged caveolae/rafts significantly enhanced ENO adhesion to HCMECs, promoted the "destruction" of RPSA by ENO, and enhanced the toxic effect of ENO on HCMECs. Importantly, these effects could be relieved upon the addition of cholesterol. We conclude that caveolae/rafts weaken the toxic effect of SS2 ENO on RPSA-mediated events in HCMECs. Our study has led to better understanding of the roles of RPSA and caveolae/rafts upon SS2 infection, and a new pathological role for RPSA in infection.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.vetmic.2021.108981DOI Listing
March 2021

Nuclear-Encoded lncRNA Epigenetically Controls Metabolic Reprogramming in HCC Cells through the Mitophagy Pathway.

Mol Ther Nucleic Acids 2021 Mar 4;23:264-276. Epub 2020 Oct 4.

Key Laboratory of Organ Regeneration and Transplantation of Ministry of Education, Cancer Center, The First Hospital of Jilin University, Changchun, Jilin 130021, China.

Mitochondrial dysfunction is a metabolic hallmark of cancer cells. In search of molecular factors involved in this dysregulation in hepatocellular carcinoma (HCC), we found that the nuclear-encoded long noncoding RNA (lncRNA) (metastasis-associated lung adenocarcinoma transcript 1) was aberrantly enriched in the mitochondria of hepatoma cells. Using RNA reverse transcription-associated trap sequencing (RAT-seq), we showed that interacted with multiple loci on mitochondrial DNA (mtDNA), including D-loop, , , and genes. knockdown induced alterations in the CpG methylation of mtDNA and in mitochondrial transcriptomes. This was associated with multiple abnormalities in mitochondrial function, including altered mitochondrial structure, low oxidative phosphorylation (OXPHOS), decreased ATP production, reduced mitophagy, decreased mtDNA copy number, and activation of mitochondrial apoptosis. These alterations in mitochondrial metabolism were associated with changes in tumor phenotype and in pathways involved in cell mitophagy, mitochondrial apoptosis, and epigenetic regulation. We further showed that the RNA-shuttling protein HuR and the mitochondria transmembrane protein MTCH2 mediated the transport of in this nuclear-mitochondrial crosstalk. This study provides the first evidence that the nuclear genome-encoded lncRNA functions as a critical epigenetic player in the regulation of mitochondrial metabolism of hepatoma cells, laying the foundation for further clarifying the roles of lncRNAs in tumor metabolic reprogramming.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.omtn.2020.09.040DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7773746PMC
March 2021

Advancement in sequencing the mitochondrial genome of Wei, 1994 (Hymenoptera: Tenthredinidae) and the phylogenetic classification of Fenusini.

Mitochondrial DNA B Resour 2019 Nov 20;4(2):4100-4101. Epub 2019 Nov 20.

College of Life Sciences, Jiangxi Normal University, Nanchang, Jiangxi, China.

The nearly complete mitochondrial genome of Wei, 1994 has been sequenced and the genome was revised with more comprehensively sequenced to near completion. The new mitogenome sequences were constructed using two separate assembly approaches, both yielding consistent results. Compared with the sequence previously reported (MF197548.1), the () and () genes were assembled, and the (+)- () genes were rearranged compared with the ancestral type. The systematic classification of was examined to provide a basis for allocation into Tenthredinidae phylogeny.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/23802359.2019.1692728DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7707670PMC
November 2019

The complete chloroplast genome sequence of , an endangered Chinese medicinal plant ().

Mitochondrial DNA B Resour 2019 Nov 13;4(2):4055-4056. Epub 2019 Nov 13.

College of Traditional Chinese Medicine, Hebei University, Baoding, PR China.

is a valuable medicinal plant endemic to the Qinghai-Tibetan Plateau. It has been listed classified under the IUCN Red List categories of Vulnerable due to the low reproductive rate and heavy exploitation. In this study, the complete chloroplast (cp) genome of has been assembled using data from the whole-genome Illumina sequencing. The cp genome is 161,515 bp in size and contains two inverted repeat regions of 30,823 bp each, which is separated by a large single-copy region of 86,675 bp and a small single-copy region of 13,194 bp. The cp genome contains 87 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. Phylogenetic analysis revealed that the cp genome of was closely related to that of the .
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/23802359.2019.1688722DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7707752PMC
November 2019

An improved statistical approach for reconstructing past climates from biotic assemblages.

Proc Math Phys Eng Sci 2020 Nov 25;476(2243):20200346. Epub 2020 Nov 25.

Ministry of Education Key Laboratory for Earth System Modelling, Department of Earth System Science, Tsinghua University, Beijing 100084, People's Republic of China.

Quantitative reconstructions of past climates are an important resource for evaluating how well climate models reproduce climate changes. One widely used statistical approach for making such reconstructions from fossil biotic assemblages is weighted averaging partial least-squares regression (WA-PLS). There is however a known tendency for WA-PLS to yield reconstructions compressed towards the centre of the climate range used for calibration, potentially biasing the reconstructed past climates. We present an improvement of WA-PLS by assuming that: (i) the theoretical abundance of each taxon is unimodal with respect to the climate variable considered; (ii) observed taxon abundances follow a multinomial distribution in which the total abundance of a sample is climatically uninformative; and (iii) the estimate of the climate value at a given site and time makes the observation most probable, i.e. it maximizes the log-likelihood function. This climate estimate is approximated by weighting taxon abundances in WA-PLS by the inverse square of their climate tolerances. We further improve the approach by considering the frequency ( ) of the climate variable in the training dataset. Tolerance-weighted WA-PLS with correction greatly reduces the compression bias, compared with WA-PLS, and improves model performance in reconstructions based on an extensive modern pollen dataset.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1098/rspa.2020.0346DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7735294PMC
November 2020

Increasing Imbalance of Treg/Th17 Indicates More Severe Glucose Metabolism Dysfunction in Overweight/obese Patients.

Arch Med Res 2021 Apr 13;52(3):339-347. Epub 2020 Dec 13.

Department of Endocrinology, Xiangya Hospital, Central South University, Changsha, Hunan, China. Electronic address:

Background: Chronic low-grade inflammation and dysfunction of metabolism has been reported to be involved in obesity. Regulatory T cell (Treg) and helper T cell 17 (Th17) are involved in chronic inflammatory diseases. Impaired balance of Treg/Th17 is one of the major factors contributing to inflammatory status in obesity.

Methods: Overweight/obese patients (n = 80) were recruited and classified into three subgroups: normal glucose tolerance group (NGT, n = 32), impaired glucose regulation group (IGR, n = 19) and type two diabetes mellitus group (T2DM, n = 29). Healthy individuals were paired as normal control group (NC, n = 37). We used flow cytometry to test the frequencies of circulating Treg and Th17 cells of all subjects. Serum IL-6, IL-10, TNF-α, IL-17A levels were detected by cytometric bead array and clinical information was extracted from medical records.

Results: In group IGR and T2DM, we revealed a severe decrease in peripheral ratio of Treg/Th17 compared with NC, but no significant difference was seen in group NGT. The serum level of IL-6 in group NGT and T2DM was higher than healthy subjects. The FPG and HbA1c levels were negatively correlated with the ratio of Treg/Th17 in overweight/obese patients. ROC curve analysis revealed that peripheral Treg/Th17 ratio <1.255 was a risk factor for prediabetes and diabetes in overweight/obese patients.

Conclusion: Peripheral Treg/Th17 imbalance exists in overweight/obese patients with IGR or T2DM and peripheral Treg/Th17 imbalance might be a risk factor for prediabetes and diabetes in overweight/obese patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.arcmed.2020.11.012DOI Listing
April 2021

Tracking endocytosis and intracellular distribution of spherical nucleic acids with correlative single-cell imaging.

Nat Protoc 2021 01 7;16(1):383-404. Epub 2020 Dec 7.

School of Chemistry and Chemical Engineering, Frontiers Science Center for Transformative Molecules, Institute of Translational Medicine, Shanghai Jiao Tong University, Shanghai, China.

A comprehensive understanding of interactions between nanoparticles (NPs) and biological components is critical to the clinical application of NPs and nanomedicine. Here we provide a step-by-step correlative imaging approach to investigate plasmonic NPs of different aggregation states at the single-cell level. Traceable spherical nucleic acids (SNAs) are fabricated by decorating 50-nm spherical gold NPs with fluorophore-labeled DNA, serving as dually emissive (fluorescent and plasmonic) NPs. The in situ correlative imaging with dark-field microscopy (DFM) and fluorescence microscopy (FM) reveals intracellular distribution of SNAs, whereas DFM combined with scanning electron microscopy (SEM) allows semi-quantification of SNA clustering states in solution. The imaging data are analyzed by ImageJ and a colorimetry-based algorithm written in Python. The clustering states of SNAs in a single cell can be efficiently distinguished within 20 s. This method can be readily installed to monitor real-time endocytosis and cellular distribution of plasmonic NPs of different aggregation states and to quantitatively image targets of interest (e.g., specific DNA, messenger RNA, peptides or proteins) in living cells. The entire procedure can be completed in 3-5 d and requires standard DFM, FM and SEM imaging and data analysis skills and equipment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41596-020-00420-1DOI Listing
January 2021

RPSA distribution and expression in tissues and immune cells of pathogen-infected mice.

Microb Pathog 2021 Mar 17;152:104609. Epub 2020 Nov 17.

The Clinical Laboratory Department of First Hospital, Jilin University, Changchun, China; College of Veterinary Medicine, Jilin University, Changchun, China; College of Animal Sciences, Yangtze University, Jingzhou, China. Electronic address:

Aims: 40S ribosomal protein SA (RPSA), a component of the small ribosomal subunit, is a high-affinity receptor of laminin that is widely expressed in cells and involves in many biological processes. However, it hasn't been reported which tissues and cells may be targeted by RPSA-mediated pathogen regulation. Therefore, in this study, a gram-positive bacterium Streptococcus suis Type 2 (SS2), gram-negative bacterium Actinobacillus pleuropneumoniae (A.pleuropneumoniae), and porcine circovirus Type 2 (PCV2) were used to infect ICR mice.

Methods And Results: The effects of infection with the three pathogens on expression levels of RPSA in mouse tissues and peripheral blood immune cells were analysed by immunohistochemistry and flow cytometry. The results suggested that the pathological changes in mice infected with SS2 were mainly manifested as congestion and inflammatory infiltration in the meninges, lungs, hearts and livers. The mice infected with A.pleuropneumoniae or PCV2 showed lung lesions and mild hepatocyte degeneration, respectively. In uninfected mice, RPSA protein was expressed to various degrees in all tissues except the spleen. After SS2 infection for 3 d, the expression of RPSA in the liver and brain increased, while decreased significantly in the heart and duodenum. These results were corroborated on examining the correlation between RPSA expression and the process of SS2 infection, except that there was no significant difference between the expression levels in the heart at 1 d and 3 d. After A.pleuropneumoniae and PCV2 infection for 3 d, the expression of RPSA decreased in the heart, and brain, respectively. Additionally, under physiological conditions, RPSA expression in CD4 T cells, CD8 T cells, neutrophils, and macrophages in the peripheral blood of mice was higher than that in B cells and NK cells. After SS2 infection for 3 d, RPSA expression increased significantly in CD4 T cells and CD8 T cells but decreased significantly in macrophages. The expression of RPSA after A.pleuropneumoniae and PCV2 infection were similar, and RPSA expression decreased only in macrophages.

Conclusions: The results revealed that RPSA showed different expression levels in tissues and immune cells due to different pathogens causing disease courses, suggesting different target tissues and target cells in RPSA-mediated pathogenesis after infection, which supports the systematic study of the pathogenesis of RPSA in infectious diseases.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.micpath.2020.104609DOI Listing
March 2021

All-Atomic Molecular Dynamic Studies of Human and CDK8: Insights into Their Kinase Domains, the LXXLL Motifs, and Drug Binding Site.

Int J Mol Sci 2020 Oct 12;21(20). Epub 2020 Oct 12.

Department of Molecular and Cellular Medicine, College of Medicine, Texas A&M University Health Science Center, 8447 Riverside Parkway, Bryan, TX 77807, USA.

Cyclin-dependent kinase 8 (CDK8) and its regulatory partner Cyclin C (CycC) play conserved roles in modulating RNA polymerase II (Pol II)-dependent gene expression. To understand the structure and function relations of CDK8, we analyzed the structures of human and CDK8 proteins using molecular dynamics simulations, combined with functional analyses in . Specifically, we evaluated the structural differences between hCDK8 and dCDK8 to predict the effects of the LXXLL motif mutation (AQKAA), the P154L mutations, and drug binding on local structures of the CDK8 proteins. First, we have observed that both the LXXLL motif and the kinase activity of CDK8 are required for the normal larval-to-pupal transition in . Second, our molecular dynamic analyses have revealed that hCDK8 has higher hydrogen bond occupation of His149-Asp151 and Asp151-Asn156 than dCDK8. Third, the substructure of Asp282, Phe283, Arg285, Thr287 and Cys291 can distinguish human and CDK8 structures. In addition, there are two hydrogen bonds in the LXXLL motif: a lower occupation between L312 and L315, and a relatively higher occupation between L312 and L316. Human CDK8 has higher hydrogen bond occupation between L312 and L316 than dCDK8. Moreover, L312, L315 and L316 in the LXXLL motif of CDK8 have the specific pattern of hydrogen bonds and geometries, which could be crucial for the binding to nuclear receptors. Furthermore, the P154L mutation dramatically decreases the hydrogen bond between L312 and L315 in hCDK8, but not in dCDK8. The mutations of P154L and AQKAA modestly alter the local structures around residues 154. Finally, we identified the inhibitor-induced conformational changes of hCDK8, and our results suggest a structural difference in the drug-binding site between hCDK8 and dCDK8. Taken together, these results provide the structural insights into the roles of the LXXLL motif and the kinase activity of CDK8 in vivo.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ijms21207511DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7590003PMC
October 2020

Alkaloid-based regimen is beneficial for acute myeloid leukemia resembling acute promyelocytic leukemia with NUP98/RARG fusion and RUNX1 mutation: A case report.

Medicine (Baltimore) 2020 Oct;99(40):e22488

Department of Hematology, Cancer Center, the First Hospital of Jilin University, Changchun.

Rationale: Some acute myeloid leukemia (AML) patients present with features mimicking the classical hypergranular subtype of acute promyelocytic leukemia (APL) but without the typical promyelocytic leukemia/retinoic acid receptor α (PML/RARα) rearrangement. Herein, we report an AML patient resembling APL but with nucleoporin 98/retinoid acid receptor gamma gene (NUP98/RARG) fusion transcript and Runt-related transcription factor 1 (RUNX1) mutation.

Patient Concerns: An 18-year-old male presented at the hospital with a diagnosis of AML.

Diagnoses: The patient was diagnosed with bone marrow examination. Bone marrow smear displayed 90.5% promyelocytes. Fluorescence in situ hybridization analysis failed to detect the PML/RARα fusion transcript or RARα amplification. While real-time polymerase chain reaction showed positivity for the NUP98/RARG fusion transcript. G-banding karyotype analysis showed a normal karyotype.

Interventions: The patient showed resistance to arsenic trioxide and standard 3 + 7 chemotherapy, but eventually achieved complete remission through the Homoharringtonine, Cytarabine, and Aclarubicin chemotherapy.

Outcomes: These measures resulted in a rapid response and disease control.

Lessons: Acute myeloid leukemia with the NUP98/RARG fusion gene and the RUNX1 mutation may be a special subtype of AML and may benefit from the alkaloid-based regimen.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/MD.0000000000022488DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7535657PMC
October 2020

Detection of canine parvovirus and feline panleukopenia virus in fecal samples by strand exchange amplification.

J Vet Diagn Invest 2020 Nov 30;32(6):880-886. Epub 2020 Sep 30.

Department of Pathogenic Biology, School of Basic Medicine, College of Life Sciences, Qingdao Nucleic Acid Rapid Testing International Science and Technology Cooperation Base, the Clinical Laboratory Department of the Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, P.R. China.

Canine parvovirus 2 (CPV-2) and feline panleukopenia virus (FPLV) often cause acute enteric disease in their hosts. A simple, rapid, and effective method for the on-site detection of these viruses would be useful. We used a denaturation bubble-mediated strand exchange amplification (SEA) method to successfully detect CPV-2 and FPLV in fecal samples. SEA could detect as little as 3.6 pg/μL of CPV-2 and 6.6 pg/μL of FPLV genomic DNA following a 40-min incubation at an isothermal temperature of 61°C. Unlike PCR, SEA does not require complicated equipment, and positive samples produce a color change that can be visualized by the naked eye. Additionally, SEA is simpler than PCR because no extraction is needed, and heating of the fecal sample at 98°C can be performed with a heating block or water bath. This rapid and effective nucleic acid detection platform could be used as a point-of-care test for the detection of CPV-2 and FPLV.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/1040638720962067DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7649555PMC
November 2020

REDBot: Natural language process methods for clinical copy number variation reporting in prenatal and products of conception diagnosis.

Mol Genet Genomic Med 2020 11 22;8(11):e1488. Epub 2020 Sep 22.

Human Genome Sequencing Center, Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA.

Background: Current copy number variation (CNV) identification methods have rapidly become mature. However, the postdetection processes such as variant interpretation or reporting are inefficient. To overcome this situation, we developed REDBot as an automated software package for accurate and direct generation of clinical diagnostic reports for prenatal and products of conception (POC) samples.

Methods: We applied natural language process (NLP) methods for analyzing 30,235 in-house historical clinical reports through active learning, and then, developed clinical knowledge bases, evidence-based interpretation methods and reporting criteria to support the whole postdetection pipeline.

Results: Of the 30,235 reports, we obtained 37,175 CNV-paragraph pairs. For these pairs, the active learning approaches achieved a 0.9466 average F1-score in sentence classification. The overall accuracy for variant classification was 95.7%, 95.2%, and 100.0% in retrospective, prospective, and clinical utility experiments, respectively.

Conclusion: By integrating NLP methods in CNVs postdetection pipeline, REDBot is a robust and rapid tool with clinical utility for prenatal and POC diagnosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/mgg3.1488DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7667294PMC
November 2020

A fully integrated hand-powered centrifugal microfluidic platform for ultra-simple and non-instrumental nucleic acid detection.

Talanta 2020 Nov 14;219:121221. Epub 2020 Jun 14.

Qingdao Nucleic Acid Rapid Testing International Science and Technology Cooperation Base, College of Life Sciences, Department of Pathogenic Biology, School of Basic Medicine, Qingdao University, Qingdao, 266071, PR China. Electronic address:

Hand-powered centrifugal microfluidics combined with isothermal nucleic acid amplification testing (NAAT) have been one of the most promising rapid detection platforms in resource-limited settings. However, current hand-powered centrifuges still suffer from customized instrument-based operation and low rotation rate; and most isothermal NAAT were conducted with complicated reaction systems for DNA detection and required an additional step for RNA detection. Herein, we built a fully hand-powered centrifugal miniaturized NAAT platform inspired by buzzer toys, which embedded sample preparation, strand exchange amplification (SEA) and visual fluorescence detection together. The centrifugal disc was easily fabricated, and operated the mixing in 1 min by simply dragging the looped rope through it with a mean input force of 16.5 N, enabling its rotation rate reach 5000 rpm. In addition, SEA was an ultra-simple one-step DNA or RNA detection method initiated by Bst DNA polymerase and a pair of primers, and thus we took all its merits and integrate it into microfluidic systems firstly. Furthermore, taking Vibrio parahemolyticus as an example, the microfluidic platform achieved DNA or RNA detection within 1 h; and the detection limit of the microchip for artificially spiked oysters was 10 CFU/g without cumbersome sample preparation, and reached to 10 CFU/g after enrichment. Therefore, we provided an ultra-simple and non-instrumental microfluidic platform powered merely by hands, performing general potential in sample-to-answer NAAT for versatile pathogens in remote regions.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.talanta.2020.121221DOI Listing
November 2020

Novel biomarkers in cats with congestive heart failure due to primary cardiomyopathy.

J Proteomics 2020 08 9;226:103896. Epub 2020 Jul 9.

Ross University School of Veterinary Medicine, Basseterre, St Kitts & Nevis, West Indies. Electronic address:

The pathogenesis of feline cardiomyopathy and congestive heart failure (CHF) requires further understanding. In this study, we assessed serum proteome change in feline CHF, aiming to identify novel biomarker for both research and clinical use. The study comprised 15 cats in CHF, 5 cats in preclinical cardiomyopathy and 15 cats as healthy controls. Serum proteome profiles were obtained by tandem mass tag labelling followed by mass spectrometry. Protein concentrations in CHF cats were compared with healthy controls. Western blot was performed for proteomic validation. Correlations were assessed between the altered proteins in CHF and clinical variables in cats with cardiomyopathy to evaluate protein-cardiac association. Bioinformatic analysis was employed to identify pathophysiological pathways involved in feline CHF. Sixteen serum proteins were significantly different between CHF and healthy control cats (P < .05). These included serine protease inhibitors, apolipoproteins and other proteins associated with inflammation and coagulation. Clinical parameters from cats with cardiomyopathy significantly correlated with the altered proteins (P < .05). Bioinformatic analysis identified 13 most relevant functional profiles in feline CHF, which mostly associated with extracellular matrix organization and metabolism. Data are available via ProteomeXchange with identifier PXD017761. SIGNIFICANCE: Cardiomyopathies affect both cats and humans, and they can cause serious consequence such as congestive heart failure (CHF). To date, the pathophysiological mechanism of CHF is not fully understood. In this study, for the first time, we used a proteomic approach combined with bioinformatic analysis to evaluate serum protein change in cats with CHF. Results indicate systemic inflammation, coagulation protein changes, innate immunity and extracellular matrix remodeling are involved in feline CHF, which are largely comparable with findings in previous human studies. Our study provides new insights into CHF and cardiomyopathy in cats, and the identified novel biomarkers and pathophysiological pathways provide valuable information for future studies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jprot.2020.103896DOI Listing
August 2020

Rapid DNA detection and one-step RNA detection catalyzed by Bst DNA polymerase and narrow-thermal-cycling.

Analyst 2020 Aug 10;145(15):5118-5122. Epub 2020 Jul 10.

Qingdao Nucleic Acid Rapid Testing International Science and Technology Cooperation Base, College of Life Sciences, Department of Pathogenic Biology, School of Basic Medicine, and Department of Clinical Laboratory, the Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, 266071, PR China.

We reported a novel detection method named accelerated strand exchange amplification by employing Bst DNA polymerase and narrow-thermal-cycling for the first time, achieving direct detection of 120 copies of DNA within 15 min and 1.2 × 10 copies of RNA within 20 min and sparking the revolution of the use of routine isothermal polymerases for diverse applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0an00975jDOI Listing
August 2020

Super Tough and Self-Healable Poly(dimethylsiloxane) Elastomer via Hydrogen Bonding Association and Its Applications as Triboelectric Nanogenerators.

ACS Appl Mater Interfaces 2020 Jul 29;12(28):31975-31983. Epub 2020 Jun 29.

Department of Materials Science and Engineering, National University of Singapore, 9 Engineering Drive 1, Singapore 117575, Singapore.

Poly(dimethylsiloxane) (PDMS) as one of the electron-drawing materials has been widely used in triboelectric nanogenerators (TENG), which is expected to generate electron through friction and required to endure dynamic loads. However, the nature of the siloxane bond and the low interchain interaction between the methyl side groups result in low fracture energy in PDMS elastomers. Here, a strategy that combined the advantages of the dynamic of hierarchical hydrogen bonding and phase-separation-like structure was adopted to improve the toughness of PDMS elastomers. By varying both stronger and weaker hydrogen bonding within the PDMS network, a series of super tough (up to 24,000 J/m), notch-insensitive, transparent, and autonomous self-healable elastomers were achieved. In addition, a hydrophilic polymeric material (PDMAS-U10) was synthesized as the conductive layer. A transparent TENG was fabricated by sandwiching the PDMAS-U10 between two pieces of the PDMS elastomer. Despite its hydrophilic nature, PDMAS-U10 exhibit strong adhesion interaction with hydrophobic PDMS elastomers. As such, a tough (16,500 J/m), self-healable (efficiency ∼97%), and transparent triboelectric nanogenerator was constructed. A self-powered system employing the TENG is also demonstrated in this work.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.0c08213DOI Listing
July 2020

Analysis of Gene Signatures of Tumor Microenvironment Yields Insight Into Mechanisms of Resistance to Immunotherapy.

Front Bioeng Biotechnol 2020 25;8:348. Epub 2020 May 25.

Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

The recent clinical success of immunotherapy represents a turning point in cancer management. But the response rate of immunotherapy is still limited. The inflamed tumor microenvironment has been reported to correlate with response in tumor patients. However, due to the lack of appropriate experimental methods, the reason why the immunotherapeutic resistance still existed on the inflamed tumor microenvironment remains unclear. Here, based on single-cell RNA sequencing, we classified the tumor microenvironment into inflamed immunotherapeutic responsive and inflamed non-responsive. Then, phenotype-specific genes were identified to show mechanistic differences between distant microenvironment phenotypes. Finally, we screened for some potential drugs that can convert an unfavorable microenvironment phenotype to a favorable one to aid current immunotherapy. Multiple signaling pathways were phenotypes-specific dysregulated. Compared to non-inflamed microenvironment, the expression of interleukin signaling pathways-associated genes was upregulated in inflamed microenvironment. Compared to inflamed responsive microenvironment, the PPAR signaling pathway-related genes and multiple epigenetic pathways-related genes were, respectively, suppressed and upregulated in the inflamed non-responsive microenvironment, suggesting a potential mechanism of immunotherapeutic resistance. Interestingly, some of the identified phenotype-specific gene signatures have shown their potential to enhance the efficacy of current immunotherapy. These results may contribute to the mechanistic understanding of immunotherapeutic resistance and guide rational therapeutic combinations of distant targeted chemotherapy agents with immunotherapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fbioe.2020.00348DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7263059PMC
May 2020

Proteomics analysis of important molecules in serum from meningitic piglets caused by Streptococcus suis serotype 2.

J Infect Dev Ctries 2020 05 31;14(5):502-510. Epub 2020 May 31.

College of Veterinary Medicine, Jilin University, Changchun, China.

Introduction: Streptococcus suis serotype 2 (SS2) is an important zoonotic pathogen that causes meningitis in China. This study's aim was comparative analysis of serum proteomics from meningitis and non-meningitis piglets.

Methodology: SS2 meningitis and non-meningitis piglet models were established. The serum samples were collected and analyzed by label-free LC-MS/MS proteomics technology. Differentially expressed proteins (DEPs) from serum were screened out by comparing the meningitis group and non-meningitis group to the healthy group (M/C; N/C), respectively. And then, globally and comparative analysis of DEPs in "M/C" and "N/C" in serum were performed using bioinformatics method. Finally, we comparatively analyzed the serum and cerebrospinal fluid proteomics in piglets that lived with meningitis.

Results: We obtained 316 and 191 DEPs from "M/C" and "N/C" which classification visualizations were established. 157 DEPs were common in both groups and 159 DEPs were unique to the "M/C". These DEPs and the signaling pathways which they participated in were visualized. Moreover, some DEPs which participated in multiple pathways were discovered and the interaction between 159 DEPs was also mapped. 39 common DEPs were also screened out in serum and cerebrospinal fluid during meningitis, and signaling pathways associated with these DEPs were further visualized.

Conclusions: DEPs in "M/C" and "N/C" were comparatively analyzed and the similarities and differences of these DEPS which were involved in signal pathways were summarized. Moreover, several important molecules were screened out.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3855/jidc.12100DOI Listing
May 2020

Alveolar soft part sarcoma of the tongue: a case report and review of the literature.

Int J Clin Exp Pathol 2020 1;13(5):1275-1282. Epub 2020 May 1.

Department of Pathology, The First Affiliated Hospital of Bengbu Medical College, Bengbu Medical College Bengbu 233004, Anhui, China.

Alveolar soft part sarcoma (ASPS) is a soft tissue malignant tumor of unknown origin in which tissues or cells are arranged like acini or organs. It usually presents in the deep muscles or fascia of the extremities, and rarely occurs in the head and neck, let alone the tongue. To our knowledge, only 49 cases of ASPS have been previously published in the tongue. Therefore, the course, age of onset, tumor size, and prognosis of ASPS in the tongue are not well understood. We present a case of ASPS in the dorsum of left tongue of a 24-year-old female. Histology of the resected tumor showed features of ASPS. She is currently disease-free at 12-month follow-up.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7270675PMC
May 2020

Programming Biomimetically Confined Aptamers with DNA Frameworks.

ACS Nano 2020 07 5;14(7):8776-8783. Epub 2020 Jun 5.

Institute of Molecular Medicine, Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China.

Active sites of proteins are generally encapsulated within three-dimensional peptide scaffolds that provide the molecular-scale confinement microenvironment. Nevertheless, the ability to tune thermodynamic stability in biomimetic molecular confinement relies on the macromolecular crowding effect of lack of stoichiometry and reconfigurability. Here, we report a framework nucleic acid (FNA)-based strategy to increase thermodynamic stability of aptamers. We demonstrate that the molecular-scale confinement increases the thermodynamic stability of aptamers facilitated folding kinetics, which is confirmed by the single-molecule FRET (smFRET). Unfavorable conformations of aptamers are restricted as revealed by the Monte Carlo simulation. The binding affinity of the DNA framework-confined aptamer is improved by ∼3-fold. With a similar strategy we improve the catalytic activity of hemin-binding aptamer. Our approach thus shows high potential for designing protein-mimicking DNA nanostructures with enhanced binding affinity and catalytic activity for biosensing and biomedical engineering.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsnano.0c03362DOI Listing
July 2020