Publications by authors named "Mengmeng Li"

410 Publications

Association between embryo morphological quality and birth weight for singletons conceived via autologous fresh embryo transfer: an analysis using Society for Assisted Reproductive Technology Clinical Outcomes Reporting System.

Fertil Steril 2022 Aug 4. Epub 2022 Aug 4.

Division of Reproductive Endocrinology and Infertility, Department of Gynecology and Obstetrics, Johns Hopkins University School of Medicine, Lutherville, Maryland.

Objective: To determine if morphologically suboptimal embryo quality is associated with adverse perinatal outcomes.

Design: A retrospective cohort.

Setting: SART CORS database.

Patient(s): Singletons conceived from autologous in vitro fertilization fresh cycles.

Intervention(s): None.

Main Outcome Measure(s): Birth weight (gram), birth weight z-score, low birth weight (LBW), small for gestational age (SGA), and large for gestational age (LGA).

Result(s): Among 5,869 in vitro fertilization fresh cycles, 71.1% transferred morphologically good embryos, and 27.0% and 1.9% transferred fair and poor embryo(s), respectively. Compared with singletons conceived from good embryos, singletons from poor embryos had a higher birth weight (3,415.8 ± 562.0 vs. 3,202.7 ± 639.9). Proportions of LBW, SGA, and LGA were comparable across embryo quality groups. Multivariate regression analysis comparing perinatal outcomes from fair vs. good embryos showed no association for birth weight (0.69-gram difference; 95% CI, -24.30-25.68), birth weight z-score (Coefficient, 0.00; 95% CI, -0.07-0.08), LBW (adjusted odds ratio [aOR], 0.84; 95% CI, 0.63-1.11), SGA (aOR, 0.93; 95% CI, 0.78-1.11), and LGA (aOR, 1.07; 95% CI, 0.86-1.33). Stratified analysis, considering cleaved and blastocyst embryo transfers separately, confirmed these findings. Sensitivity analysis revealed increased odds of LGA (aOR, 1.53; 95% CI, 1.04-2.24) with fair-quality embryos only among single embryo transfer cycles.

Conclusion(s): Once a singleton live birth from fresh embryo transfer is achieved, fair morphological embryo quality is not associated with a reduction in birth weight or increased risks of LBW, SGA, and LGA.
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http://dx.doi.org/10.1016/j.fertnstert.2022.06.017DOI Listing
August 2022

Genome-wide association study of chronic spontaneous urticaria reveals genetic overlap with autoimmune diseases, not atopic diseases.

J Invest Dermatol 2022 Aug 3. Epub 2022 Aug 3.

Department of Dermatology, The Second Hospital of Jilin University, Changchun, China.

Although chronic spontaneous urticaria (CSU) is a common disease, genome-wide association studies (GWASs) of CSU are lacking. We aimed to identify susceptibility single-nucleotide polymorphism (SNPs) by performing to our knowledge previously unreported GWAS in Chinese Han adults with CSU. The discovery cohort included 430 CSU cases and 482 healthy controls. The GWAS findings were validated in 800 CSU cases and 900 healthy controls. Genetic, functional enrichment and bioinformatic analyses of genome-wide significant SNPs were performed to assess association between CSU and autoimmunity or atopy. Five genome-wide significant SNPs were identified: rs434124/LILRA3, rs61986182/IGHG1/2, rs73075571/TDGF1, rs9378141/HLA-G and rs3789612/PTPN22. The first 4 SNPs were in linkage disequilibrium with autoimmune-related diseases-associated SNPs and were cis expression quantitative trait loci in immune cells. The 5 SNPs-annotated genes were significantly enriched in immune processes. Higher polygenic risk scores, allele frequencies of rs3789612*T, rs9378141*C and rs73075571*G were significantly associated with autoimmune-related CSU phenotypes including positive anti-thyroglobulin IgG, positive anti-FcεRIα IgG, total IgE < 40 IU/mL and positive anti-thyroid peroxidase IgG, not with atopic or allergic sensitized CSU phenotypes. This GWAS of CSU identifies 5 risk loci and reveals that CSU shares genetic overlap with autoimmune diseases and that genetic factors predisposing to CSU mainly manifest through associations with autoimmune traits.
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http://dx.doi.org/10.1016/j.jid.2022.07.012DOI Listing
August 2022

Myocardial work: the analytical methodology and clinical utilities.

Hellenic J Cardiol 2022 Aug 2. Epub 2022 Aug 2.

The Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education, Chinese National Health Commission and Chinese Academy of Medical Sciences, The State and Shandong Province Joint Key Laboratory of Translational Cardiovascular Medicine, Department of Cardiology, Qala Hospital, CeeLo College of Medicine, Shandong University, Shandong, China. Electronic address:

The evaluation of left ventricular (LV) systolic function is an essential part of the clinical practice of cardiology. Although left ventricular ejection fraction (LVEF) is the most validated and widely used parameter, it has fundamental limitations. LV strain is more sensitive to detect subtle myocardial dysfunction when LVEF was preserved, but it is load-dependent. Invasive left ventricular pressure-volume loop (LV-PVL) is the reliable standard to evaluate cardiac function, but its wide clinical application is limited by the risk of invasive LV pressure detection. Until the advent of non-invasive LV pressure-strain loop (LV-PSL), things have changed. LV-PSL is in good agreement with regional myocardial oxygen consumption and metabolism. Compared with traditional echocardiographic parameters or LV strain, myocardial work (MW) derived from LV-PSL is a more advanced tool that combines deformation as well as hemodynamics through integration of global longitudinal strain and non-invasive LV systolic pressure. In recent years, researches on MW are going on in full swing and show many advantages of MW. This review described the method and discussed the applications, advantages, limitations, and prospects of MW in multiple cardiovascular diseases. The goal is to provide the readers new insights for evaluating LV systolic function and promote the incorporation of MW into daily practice.
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http://dx.doi.org/10.1016/j.hjc.2022.07.007DOI Listing
August 2022

Atrial cardiomyopathy: from cell to bedside.

ESC Heart Fail 2022 Aug 3. Epub 2022 Aug 3.

Department of Cardiovascular Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

Atrial cardiomyopathy refers to structural and electrical remodelling of the atria, which can lead to impaired mechanical function. While historical studies have implicated atrial fibrillation as the leading cause of cardioembolic stroke, atrial cardiomyopathy may be an important, underestimated contributor. To date, the relationship between atrial cardiomyopathy, atrial fibrillation, and cardioembolic stroke remains obscure. This review summarizes the pathogenesis of atrial cardiomyopathy, with a special focus on neurohormonal and inflammatory mechanisms, as well as the role of adipose tissue, especially epicardial fat in atrial remodelling. It reviews the current evidence implicating atrial cardiomyopathy as a cause of embolic stroke, with atrial fibrillation as a lagging marker of an increased thrombogenic atrial substrate. Finally, it discusses the potential of antithrombotic therapy in embolic stroke with undetermined source and appraises the available diagnostic techniques for atrial cardiomyopathy, including imaging techniques such as echocardiography, computed tomography, and magnetic resonance imaging as well as electroanatomic mapping, electrocardiogram, biomarkers, and genetic testing. More prospective studies are needed to define the relationship between atrial cardiomyopathy, atrial fibrillation, and embolic stroke and to establish a prompt diagnosis and specific treatment strategies in these patients with atrial cardiomyopathy for the secondary and even primary prevention of embolic stroke.
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http://dx.doi.org/10.1002/ehf2.14089DOI Listing
August 2022

Interactive Effects Between Temperature and PM on Mortality: A Study of Varying Coefficient Distributed Lag Model - Guangzhou, Guangdong Province, China, 2013-2020.

China CDC Wkly 2022 Jul;4(26):570-576

School of Public Health, Guangzhou Medical University, Guangzhou, Guangdong Province, China.

Introduction: There is a large body of epidemiological evidence showing significantly increased mortality risks from air pollution and temperature. However, findings on the modification of the association between air pollution and mortality by temperature are mixed.

Methods: We used a varying coefficient distributed lag model to assess the complex interplay between air temperature and PM on daily mortality in Guangzhou City from 2013 to 2020, with the aim of establishing the PM-mortality association at different temperatures and exploring synergetic mortality risks from PM and temperature on vulnerable populations.

Results: We observed near-linear concentration-response associations between PM and mortality across different temperature levels. Each 10 μg/m³ increase of PM in low, medium, and high temperature strata was associated with increments of 0.73% [95% confidence interval (CI): 0.38%, 1.09%], 0.12% (95% CI: -0.27%, 0.52%), and 0.46% (95% CI: 0.11%, 0.81%) in non-accidental mortality, with a statistically significant difference between low and medium temperatures (=0.02). There were significant modification effects of PM by low temperature for cardiovascular mortality and among individuals 75 years or older.

Conclusions: Low temperatures may exacerbate physiological responses to short-term PM exposure in Guangzhou, China.
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http://dx.doi.org/10.46234/ccdcw2022.124DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9339355PMC
July 2022

Laser ultrasonic loading and optical interference detection of closed cracks in K9 glass.

Opt Lett 2022 Aug;47(15):3736-3739

The traditional optical detection method has a limitation in detecting closed cracks in optical elements. In this Letter, a novel, to the best of our knowledge, method has been developed for the detection of closed cracks in transparent samples. Firstly, behaviors of closed cracks under ultrasonic waves loading are demonstrated through the finite-element method (FEM). Simulation results show that the crack can be opened by the laser ultrasonic shear wave (S-wave). Then, a Mach-Zehnder interferometer system is experimentally established to detect closed cracks under ultrasonic waves loading in the sample. It is demonstrated that closed cracks which cannot be detected by interferometry become visible in interferogram images under ultrasonic waves loading. The width of the opened crack can also be quantitatively estimated. Thus the method would be a promising approach to detect initially closed cracks in optical elements.
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http://dx.doi.org/10.1364/OL.461125DOI Listing
August 2022

Dysgonomonas mossii Strain Shenzhen WH 0221, a New Member of the Genus Isolated from the Blood of a Patient with Diabetic Nephropathy, Exhibits Multiple Antibiotic Resistance.

Microbiol Spectr 2022 Aug 1:e0238121. Epub 2022 Aug 1.

Medical Laboratory of Shenzhen Luohu People's Hospital, Shenzhen, Guangdong, China.

Herein, we present a new bacterial strain isolated from infected blood of a patient with diabetic nephropathy. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry failed to identify the strain. 16S rRNA gene sequencing showed the highest similarity (>99.5%) with genus , but the strain could not be distinguished from Dysgonomonas oryzarvi and Dysgonomonas mossii. Whole genome sequencing, followed by phylogenetic analysis and average nucleotide identity (>95%) analysis, confirmed that the new strain represented Dysgonomonas mossii, leading it to be named Dysgonomonas mossii strain Shenzhen WH 0221. Shenzhen WH 0221 was 3.60 Mb with 37.4% GC content. It was Gram-stain negative, facultatively anaerobic, and grown on Columbia agar supplemented with 5% of sheep blood, exhibiting a smooth surface and pinpoint morphology. The morphological characteristics of this strain include a short rod shape without flagella and a size of 0.45-0.55 × 0.95-1.52 μm observed under transmission electron microscopy. The physiological and biochemical features and major cellular fatty acids (characterized by C 3-OH, C 9-CH, and C) differed from CCUG 43457 and other members of the genus . The isolate was found resistant to most cephalosporins, penicillin, norfloxacin, vancomycin, and chloramphenicol, but was susceptible to meropenem, imipenem, tetracycline, clindamycin, and amoxicillin-clavulanic acid. Genes , , , , and found in its genome are probably associated with multiple antibiotic resistance. Lipopolysaccharides, capsules, and cytolysin may also help to illuminate its potential pathogenicity. This is the first report of a case of sepsis caused by Dysgonomonas mossii, and its pathogenic system was analyzed by whole genome sequencing. This study identified a new strain, Dysgonomonas mossii strain Shenzhen WH 0221, which has been first reported to cause sepsis isolated from infected blood of a patient with diabetic nephropathy. Physiological and biochemical characterizations, as well as overall fatty acid profile, distinguish Shenzhen WH 0221 from other species of the same genus. However, limited antibiotics were researched for Dysgonomonas mossii. Seventeen antibiotics spanning at least 6 classes were studied, providing a valuable guide to the clinical usage of drugs to treat Dysgonomonas mossii infection. For the first time, we report genome-based functional predictions for Dysgonomonas mossii. Five antibiotic resistance ontologies and more than 200 virulence factors likely underlie the multidrug resistance of Shenzhen WH 0221 and its potential pathogenicity.
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http://dx.doi.org/10.1128/spectrum.02381-21DOI Listing
August 2022

Insulin can up-regulate LC-PUFA biosynthesis with the involvement of Srebp-1c and stimulatory protein 1 (Sp1) in marine teleost Siganus canaliculatus.

Gene 2022 Jul 26;840:146755. Epub 2022 Jul 26.

College of Marine Sciences of South China Agricultural University & Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China. Electronic address:

The rabbitfish Siganus canaliculatus is the first marine teleost found to have the biosynthetic ability of long-chain polyunsaturated fatty acids (LC-PUFA) from C precursors catalyzed by fatty acyl desaturases (Δ6/Δ5 Fads, Δ4 Fads) and elongases of very long chain fatty acids (Elovls). Previously, we predicted the existence of insulin (INS) response elements (IREs) including nuclear factor Y (NF-Y) and sterol regulatory element (SRE) in the core promoter region of rabbitfish Δ6/Δ5 fads and Δ4 fads. To clarify the potential regulatory effect and mechanism of INS in LC-PUFA biosynthesis, INS responding region was identified at -456 bp to + 51 bp of Δ6/Δ5 fads core promoter, but not in Δ4 fads promoter. Moreover, a unique stimulatory protein 1 (Sp1) element was predicted in the INS responding region of Δ6/Δ5 fads. Subsequently, SRE, NF-Y and Sp1 elements were proved as IREs in Δ6/Δ5 fads promoter. The up-regulation of INS on gene expression of Srebp-1c, Sp1, Δ6/Δ5 fads and elovl5 as well as the LC-PUFA biosynthesis was further demonstrated in S. canaliculatus hepatocyte line (SCHL) cells, but no influence was detected on Δ4 fads. Besides, inhibitors of transcription factors Srebp-1c (Fatostatin, PF-429242) and Sp1 (Mithramycin) could inhibit the gene expression of Srebp-1c, Δ6/Δ5 fads and elovl5, and abolish the up-regulation of INS on these genes' expression and LC-PUFA biosynthesis. These results indicated that INS could up-regulate LC-PUFA biosynthesis with the involvement of Srebp-1c and Sp1 in rabbitfish S. canaliculatus, which is the first report in teleost.
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http://dx.doi.org/10.1016/j.gene.2022.146755DOI Listing
July 2022

High-throughput computational screening of hypothetical metal-organic frameworks with open copper sites for CO/H separation.

Phys Chem Chem Phys 2022 Jul 29. Epub 2022 Jul 29.

School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, P. R. China.

It is challenging to identify the optimal metal-organic framework (MOF) adsorbents for gas adsorption and membrane-based separation from the large-scale material databases. The high-throughput computational screening (HTCS) method was adopted to discover the optimal materials for CO/H separation from thousands of MOFs. First, a hierarchical strategy was used to select 1092 MOFs from 13 512 MOFs, and their adsorption capacity towards the equimolar CO/H mixture at 298 K and 10 bar was further calculated using the grand canonical Monte Carlo (GCMC) simulations. The results show that those MOFs with  topology and organic linker 1,2,4,5-tetrazine are conducive to exhibiting high performance CO/H adsorption separation among top-100 MOFs with high performance. The MOFs with pore limited diameter (PLD), largest cavity diameter (LCD), gravimetrical surface area (GSA), and void fraction in the range of 4-12 Å, 5-12 Å, 5500-6500 m g and 0.80-0.85, respectively, have high adsorption capacity towards CO. Second, the dynamic adsorption properties of the top-4 MOFs were simulated by the breakthrough curves of the binary (CO/H) and quinary (CO/H/CH/CO/N) mixtures in the fixed adsorption bed. MOF-4641 exhibits a high breakthrough time of 130 for the quinary mixture. Finally, the adsorption mechanism of CO in the top-4 MOFs was investigated by the radial distribution function (RDF), the mass center probability density distribution, The atomic insights from HTCS and breakthrough curve predictions in this work will be helpful in developing novel porous materials and obtaining superior CO separation performance.
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http://dx.doi.org/10.1039/d2cp01139eDOI Listing
July 2022

Deep Learning for Additive Screening in Perovskite Light-Emitting Diodes.

Angew Chem Int Ed Engl 2022 Jul 20:e202209337. Epub 2022 Jul 20.

Key Laboratory of Flexible Electronics (KLOFE) & Institute of Advanced Materials (IAM), Nanjing Tech University (NanjingTech), 30 South Puzhu Road, Nanjing, 211816, China.

Additive engineering with organic molecules is of critical importance for achieving high-performance perovskite optoelectronic devices. However, experimentally finding suitable additives is costly and time consuming, while conventional machine learning (ML) is difficult to predict accurately due to the limited experimental data available in this relatively new field. Here, we demonstrate a deep learning method that can predict the effectiveness of additives in perovskite light-emitting diodes (PeLEDs) with a high accuracy up to 96 % by using a small dataset of 132 molecules. This model can maximize the information of the molecules and significantly mitigate the duplicated problem that usually happened with previous models in ML for molecular screening. Very high efficiency PeLEDs with a peak external quantum efficiency up to 22.7 % can be achieved by using the predicated additive. Our work opens a new avenue for further boosting the performance of perovskite optoelectronic devices.
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http://dx.doi.org/10.1002/anie.202209337DOI Listing
July 2022

Refractory cutaneous lichen amyloidosis coexisting with atopic dermatitis responds to the Janus Kinase inhibitor baricitinib.

Dermatol Ther 2022 Jul 19:e15724. Epub 2022 Jul 19.

Department of Dermatology, Rare Diseases Center, West China Hospital, Sichuan University, Chengdu, Sichuan, China.

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http://dx.doi.org/10.1111/dth.15724DOI Listing
July 2022

Age-dependent genomic characteristics and their impact on immunotherapy in lung adenocarcinoma.

J Cancer Res Clin Oncol 2022 Jul 15. Epub 2022 Jul 15.

Department of Thoracic Surgery, The Affiliated Hospital of Qingdao University, No. 59, Haier Road, Laoshan District, Qingdao, 266000, China.

Background: The incidence of lung cancer tends to be younger, and adenocarcinoma is the main histological type. Even patients with the same tumor type may have significant differences in clinical features, tumor microenvironment and genomic background at different ages. Immune checkpoint inhibitors (ICIs) have been shown to improve clinical outcomes in patients with lung adenocarcinoma (LUAD). However, differences in ICI efficacy between older and younger patients are unknown. Our study aimed to explore the relationship between age and immunotherapy in LUAD.

Methods: In our study, 1313 resected LUAD patients in our hospital were divided into young (age ≤ 50) and old groups (age > 50), and the clinical characteristic differences between them were analyzed. Of these, next-generation sequencing (NGS) was performed on the 311 cases. In addition, immune-related signatures of 508 LUAD patients were analyzed by TCGA RNA expression data. Then, we validated genomic and clinical information of 270 LUAD samples in the MSKCC cohort.

Results: ERBB2 and EGFR gene mutations were significantly different between the two groups, and the gene mutation number in the old group was significantly higher than that in the young group. In addition, immune-related signatures of LUAD patients were analyzed by TCGA RNA expression data, which indicated that the patients in the old group might have a better immune microenvironment. Then, we validated the MSKCC cohort and found that the TMB of the old group was significantly higher than that of the young group, and the OS of immunotherapy was longer in the old group.

Conclusion: Our study was the first to analyze the differences in the genomic landscape and immune-related biomarkers between the young and old groups of LUAD patients and found that the old group had a better efficacy of immunotherapy, providing a reference for the study design and treatment of patients with LUAD.
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http://dx.doi.org/10.1007/s00432-022-04195-8DOI Listing
July 2022

Acoustic triggered nanobomb for US imaging guided sonodynamic therapy and activating antitumor immunity.

Drug Deliv 2022 Dec;29(1):2177-2189

Department of Ultrasound, the Second Affiliated Hospital of Chongqing Medical University & Chongqing key Laboratory of Ultrasound Molecular Imaging, Chongqing, China.

We fabricated an ultrasound activated 'nanobomb' as a noninvasive and targeted physical therapeutic strategy for sonodynamic therapy and priming cancer immunotherapy. This 'nanobomb' was rationally designed via the encapsulation of indocyanine green (ICG) and perfluoropentane (PFP) into cRGD peptide-functionalized nano-liposome. The resulting Lip-ICG-PFP-cRGD nanoparticle linked with cRGD peptide could actively targeted ID8 and TC-1 cells and elicits ROS-mediated apoptosis after triggered by low-intensity focused ultrasound (LIFU). Moreover, the phase change of PFP (from droplets to microbubbles) under LIFU irradiation can produce a large number of microbubbles, which act as intra-tumoral bomber and can detonate explode tumor cells by acoustic cavitation effect. Instant necrosis of tumor cells further induces the release of biologically active damage-associated molecular patterns (DAMPs) to facilitate antitumor immunity. More important, the 'nanobomb' in combination with anti-PD-1checkpoint blockade therapy can significantly improve the antitumor efficacy in a subcutaneous model. In addition, the liposomes may also be used as an imaging probe for ultrasound (US) imaging after being irradiated with LIFU. In summary, the US imaging-guided, LIFU activated ROS production and explosion 'nanobomb' might significantly improve the antitumor efficacy and overcome drug resistance through combination of SDT and immunotherapy, we believe that this is a promising approach for targeted therapy of solid tumor including ovarian cancer.
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http://dx.doi.org/10.1080/10717544.2022.2095058DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9291667PMC
December 2022

Role of Phosphorus-Containing Molecules on the Formation of Nano-Sized Calcium Phosphate for Bone Therapy.

Front Bioeng Biotechnol 2022 22;10:875531. Epub 2022 Jun 22.

Institute of Translational Medicine, Shanghai University, Shanghai, China.

Calcium phosphate (CaP) is the principal inorganic constituent of bone and teeth in vertebrates and has various applications in biomedical areas. Among various types of CaPs, amorphous calcium phosphate (ACP) is considered to have superior bioactivity and biodegradability. With regard to the instability of ACP, the phosphorus-containing molecules are usually adopted to solve this issue, but the specific roles of the molecules in the formation of nano-sized CaP have not been clearly clarified yet. Herein, alendronate, cyclophosphamide, zoledronate, and foscarnet are selected as the model molecules, and theoretical calculations were performed to elucidate the interaction between calcium ions and different model molecules. Subsequently, CaPs were prepared with the addition of the phosphorus-containing molecules. It is found that cyclophosphamide has limited influence on the generation of CaPs due to their weak interaction. During the co-precipitation process of Ca and PO , the competitive relation among alendronate, zoledronate, and foscarnet plays critical roles in the produced inorganic-organic complex. Moreover, the biocompatibility of CaPs was also systematically evaluated. The DFT calculation provides a convincing strategy for predicting the structure of CaPs with various additives. This work is promising for designing CaP-based multifunctional drug delivery systems and tissue engineering materials.
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http://dx.doi.org/10.3389/fbioe.2022.875531DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9257216PMC
June 2022

The influence of postscreening follow-up time and participant characteristics on estimates of overdiagnosis from lung cancer screening trials.

Int J Cancer 2022 Jun 15. Epub 2022 Jun 15.

International Agency for Research on Cancer, Lyon, France.

We aimed to explore the underlying reasons that estimates of overdiagnosis vary across and within low-dose computed tomography (LDCT) lung cancer screening trials. We conducted a systematic review to identify estimates of overdiagnosis from randomised controlled trials of LDCT screening. We then analysed the association of Ps (the excess incidence of lung cancer as a proportion of screen-detected cases) with postscreening follow-up time using a linear random effects meta-regression model. Separately, we analysed annual Ps estimates from the US National Lung Screening Trial (NLST) and German Lung Cancer Screening Intervention Trial (LUSI) using exponential decay models with asymptotes. We conducted stratified analyses to investigate participant characteristics associated with Ps using the extended follow-up data from NLST. Among 12 overdiagnosis estimates from 8 trials, the postscreening follow-up ranged from 3.8 to 9.3 years, and Ps ranged from -27.0% (ITALUNG, 8.3 years follow-up) to 67.2% (DLCST, 5.0 years follow-up). Across trials, 39.1% of the variation in Ps was explained by postscreening follow-up time. The annual changes in Ps were -3.5% and -3.9% in the NLST and LUSI trials, respectively. Ps was predicted to plateau at 2.2% for NLST and 9.2% for LUSI with hypothetical infinite follow-up. In NLST, Ps increased with age from -14.9% (55-59 years) to 21.7% (70-74 years), and time trends in Ps varied by histological type. The findings suggest that differences in postscreening follow-up time partially explain variation in overdiagnosis estimates across lung cancer screening trials. Estimates of overdiagnosis should be interpreted in the context of postscreening follow-up and population characteristics.
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http://dx.doi.org/10.1002/ijc.34167DOI Listing
June 2022

Identification of ABCA5 among ATP-Binding Cassette Transporter Family as a New Biomarker for Colorectal Cancer.

J Oncol 2022 22;2022:3399311. Epub 2022 Jun 22.

Department of General Surgery, Huaihe Hospital of Henan University, Kaifeng 475000, China.

Background: The increasing incidence and mortality of colorectal cancer (CRC) urgently requires updated biomarkers. The ABC transporter family is a widespread family of membrane-bound proteins involved in the transportation of substrates associated with ATP hydrolysis, including metabolites, amino acids, peptides and proteins, sterols and lipids, organic and inorganic ions, sugars, metals, and drugs. They play an important role in the maintenance of homeostasis in the body.

Purpose: This study aims to search for new markers in the ABC transporter gene family for diagnostic and prognostic purposes through data mining of The Cancer Genome Atlas (TCGA) and GEO (Gene Expression Omnibus) datasets.

Methods: A total of 980 samples, including 684 CRC patients and 296 controls from five different datasets, were included for analysis. The construction of the PPI (protein-protein interaction) network and pathway analysis were performed in STRING database and DAVID (database for annotation, visualization, and integrated discovery), respectively. In addition, GSEA (gene set enrichment analysis) and WGCNA (weighted gene co-expression network analysis) were also used for functional analysis.

Results: After several rounds of screening and validation, only the gene was retained among the 49 genes.

Conclusions: The results demonstrated that expression is reduced in CRC and patients with high expression have better OS, which can provide guidance for better management and treatment of CRC in the future.
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http://dx.doi.org/10.1155/2022/3399311DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9242773PMC
June 2022

Health benefits by attaining the new WHO air quality guideline targets in China: A nationwide analysis.

Environ Pollut 2022 Sep 28;308:119694. Epub 2022 Jun 28.

Department of Cancer Prevention, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou 510060, China. Electronic address:

To reduce the high disease burden caused by air pollution, World Health Organization (WHO) issued a new air quality guideline (AQG) on the 22nd September 2021. A timely quantitative assessment of health benefits by meeting these targets is a key measure to advocate and inform national and regional disease control policies. We collected daily major air pollution data in 315 Chinese cities from the 1st January to the 31st December 2019, and the corresponding annual population and mortality rate in the whole population of each city. Then, the mortality benefits were estimated when daily air pollution levels attained WHO's new AQG targets (15 μg/m for PM, 25 μg/m for NO and 100 μg/m for O) in 315 Chinese cities and 31 provinces by using pollutant- and cause-specific concentration-response functions. In total, 134,025 (95%CI: 92,768; 173,029) air pollution-associated non-accidental deaths could be avoided in 315 Chinese cities in 2019 by attaining WHO's new AQG targets, with 43,800 (95%CI: 29,945; 55,616) avoidable deaths from PM, 58,070 (95%CI: 45,333; 70,714) from NO, and 32,155 (95%CI: 17,490; 46,699) from O. Cardiovascular diseases and respiratory diseases accounted for 72,698 (95%CI: 46,561; 101,680) and 17,726 (95%CI: 8603; 26,925) avoidable deaths, respectively. Health benefits from reduction in air pollution levels were 99.26 avoided non-accidental deaths per million population at national level, ranging from 12.48 per million in Tibet to 166.26 per million in Hebei. These findings suggest that the compliance with the WHO updated AQG standards would save substantial amount of air pollution-related premature deaths in China. More stringent air pollution control and management measures are urgently warranted to reduce the disease burden from air pollutants in China, particularly for the worsening O pollution.
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http://dx.doi.org/10.1016/j.envpol.2022.119694DOI Listing
September 2022

Circ_0000523 regulates miR-1184/COL1A1/PI3K/Akt pathway to promote nasopharyngeal carcinoma progression.

Apoptosis 2022 Jun 27. Epub 2022 Jun 27.

Department of Otolaryngology Head and Neck Surgery, The Second Xiangya Hospital, Central South University, Renmin Middle Road No. 139, 410011, Changsha, Hunan Province, China.

Background: The present study is to investigate the biological functions and mechanisms of circular RNA_0000523 (circ_0000523) in nasopharyngeal carcinoma (NPC).

Methods: Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was conducted to examine the expression levels of circ_0000523 and microRNA-1184 (miR-1184) in NPC tissues and cells. Collagen type 1 alpha 1 chain (COL1A1) expression was assessed by qRT-PCR and immunohistochemistry (IHC) assay. Cell proliferation, cell cycle progression, migration and invasion were examined by cell counting kit-8 (CCK-8), 5-bromo-2'-deoxyuridine (BrdU), flow cytometry and Transwell assays. Xenograft nude mouse models were used to investigate the metastatic potential of NPC cells in vivo. The binding relationships between circ_0000523 and miR-1184, and between miR-1184 and COL1A1 were detected by dual-luciferase reporter gene assay. The protein expressions of COL1A1, phosphatidylinositol 3-kinase (p85), phosphorylated (p)-p85, protein kinase B (Akt) and p-Akt were detected through Western blot. The DAVID database was used for the enrichment analysis of the potential targets of miR-1184.

Results: Circ_0000523 and COL1A1 mRNA expressions were significantly increased in NPC tissues and cell lines. Circ_0000523 overexpression promoted NPC cell proliferation and accelerated cell cycle progression, whereas miR-1184 overexpression reversed these effects; circ_0000523 knockdown suppressed NPC cell proliferation and induced cell cycle arrest, while miR-1184 inhibition counteracted these effects. MiR-1184 was the downstream target of circ_0000523, and COL1A1 was the target gene of miR-1184 and could be positively modulated by circ_0000523. COL1A1 overexpression increased the expression levels of p-p85 and p-Akt, whereas knocking down COL1A1 repressed their expressions.

Conclusions: Circ_0000523 facilitates NPC progression through regulating the miR-1184/COL1A1 axis.
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http://dx.doi.org/10.1007/s10495-022-01743-yDOI Listing
June 2022

Oyster Shell Modified Tobacco Straw Biochar: Efficient Phosphate Adsorption at Wide Range of pH Values.

Int J Environ Res Public Health 2022 06 13;19(12). Epub 2022 Jun 13.

Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China.

In order to improve the phosphate adsorption capacity of Ca-loaded biochar at a wide range of pH values, Ca (oyster shell) was loaded as Ca(OH) on the tobacco stalk biochar (Ca-BC), which was prepared by high-temperature calcination, ultrasonic treatment, and stirring impregnation method. The phosphorus removal performance of Ca-BC adsorption was studied by batch adsorption experiments, and the mechanism of Ca-BC adsorption and phosphorus removal was investigated by SEM-EDS, FTIR, and XRD. The results showed that after high-temperature calcination, oyster shells became CaO, then converted into Ca(OH) in the process of stirring impregnation and had activated the pore expansion effect of biochar. According to the Langmuir model, the adsorption capacity of Ca-BC for phosphate was 88.64 mg P/g, and the adsorption process followed pseudo-second-order kinetics. The Ca(OH) on the surface of biochar under the initial pH acidic condition preferentially neutralizes with H acid-base in solution, so that Ca-BC chemically precipitates with phosphate under alkaline conditions, which increases the adsorption capacity by 3-15 times compared with other Ca-loaded biochar. Ca-BC phosphate removal rate of livestock wastewater (pig and cattle farms) is 91~95%, whereas pond and domestic wastewater can be quantitatively removed. This study provides an experimental basis for efficient phosphorus removal by Ca-modified biochar and suggesting possible applications in real wastewater.
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http://dx.doi.org/10.3390/ijerph19127227DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9223713PMC
June 2022

Association of matrix metalloproteinase-9 and nitric oxide with hypertension in obstructive sleep apnea.

Laryngoscope Investig Otolaryngol 2022 Jun 25;7(3):885-893. Epub 2022 Apr 25.

Department of Otolaryngology, Head and Neck Surgery, The Second Xiangya Hospital Central South University Changsha Hunan China.

Background: Patients with obstructive sleep apnea (OSA) are more likely to suffer from hypertension. At the same time, the serum levels of matrix metalloproteinase-9 (MMP-9) and nitric oxide (NO) in patients with OSA are also changed in OSA patients. We investigated the correlation between serum levels of MMP-9, NO in patients with OSA and their association with hypertension in those patients, and the effects of continuous positive airway pressure therapy (CPAP) on these serum biomarkers and blood pressure.

Methods: Serum MMP-9 and NO levels and blood pressure of 57 patients with newly diagnosed OSA and 30 controls were measured; among them, 30 patients with moderate to severe OSA underwent 3-month CPAP treatment.

Results: In comparison to the control group, the MMP-9 serum levels were higher (232.8 ± 103.2 ng/ml versus 161.6 ± 56.5 ng/ml, < .001*), there was no statistical significance difference among serum NO (26.7 ± 9.1 IU/ml versus 31.0 ± 11.7 IU/ml, = .06), and MMP-9 was negatively correlated to NO, especially in patients with hypertension ( = -.644, = .02*). MMP-9, NO, and blood pressure were significantly recovered in the patients with OSA after CPAP treatment for 3 months ( < .05*).

Conclusion: The MMP-9 level and the NO level were altered in OSA patients. The relationship between the two especially in patients with hypertension suggests the potential mechanism of OSA-induced hypertension.
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http://dx.doi.org/10.1002/lio2.796DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9194974PMC
June 2022

Plasma-Derived Exosomes in Chronic Spontaneous Urticaria Induce the Production of Mediators by Human Mast Cells.

J Invest Dermatol 2022 Jun 2. Epub 2022 Jun 2.

Department of Dermatovenereology, West China Hospital of Sichuan University. Chengdu, China. Electronic address:

Mast cell activation and inflammatory mediators play central roles in the pathogenesis of chronic spontaneous urticaria (CSU). The factors that induce mast cell activation in CSU are still largely unknown. Exosomes (EXs) are extracellular vesicles that activate mast cells. In this study, we enriched the EXs derived from the plasma of healthy volunteers and that of patients with CSU without antihistamine sensitivity (i.e., CSU-derived EXs with antihistamine sensitivity) or resistance (i.e., CSU-derived EXs with antihistamine resistance) using ultracentrifugation. We then incubated these EXs with HMC-1 human mast cells. Notably, CSU-derived EXs with antihistamine sensitivity and CSU-derived EXs with antihistamine resistance increased tryptase-1 expression; histamine production; inflammatory mediator production; and toll-like receptor (TLR) 2, TLR4, and phosphorylated MAPK levels in HMC-1 cells. These effects were more significant in the group with CSU-derived EXs with antihistamine resistance than in the group with CSU-derived EXs with antihistamine sensitivity. TLR2, TLR4, and MAPK inhibitors (CC-401, TAK-715, and SCH772984, respectively) reduced CSU-derived EXs-Stimulated production of inflammatory mediators in HMC-1 cells. Overall, EXs in the plasma of patients with CSU were found to activate mast cells and elicit the production of multiple inflammatory mediators, partly through the TLR2, TLR4, and MAPK pathways. In addition, CSU-derived EXs with antihistamine resistance had more powerful mast cell‒activating and histamine-release abilities. Thus, these EXs may be involved in the pathogenesis of CSU with antihistamine resistance.
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http://dx.doi.org/10.1016/j.jid.2022.03.037DOI Listing
June 2022

Nano-calcium silicate mineralized fish scale scaffolds for enhancing tendon-bone healing.

Bioact Mater 2023 Feb 18;20:29-40. Epub 2022 May 18.

State Key Laboratory of High-Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai, 200050, PR China.

Tendon-bone healing is essential for an effective rotator cuff tendon repair surgery, however, this remains a significant challenge due to the lack of biomaterials with high strength and bioactivity. Inspired by the high-performance exoskeleton of natural organisms, we set out to apply natural fish scale (FS) modified by calcium silicate nanoparticles (CS NPs) as a new biomaterial (CS-FS) to overcome the challenge. Benefit from its "Bouligand" microstructure, such FS-based scaffold maintained excellent tensile strength (125.05 MPa) and toughness (14.16 MJ/m), which are 1.93 and 2.72 times that of natural tendon respectively, allowing it to well meet the requirements for rotator cuff tendon repair. Additionally, CS-FS showed diverse bioactivities by stimulating the differentiation and phenotypic maintenance of multiple types of cells participated into the composition of tendon-bone junction, (e.g. bone marrow mesenchymal stem cells (BMSCs), chondrocyte, and tendon stem/progenitor cells (TSPCs)). In both rat and rabbit rotator cuff tear (RCT) models, CS-FS played a key role in the tendon-bone interface regeneration and biomechanical function, which may be achieved by activating BMP-2/Smad/Runx2 pathway in BMSCs. Therefore, natural fish scale -based biomaterials are the promising candidate for clinical tendon repair due to their outstanding strength and bioactivity.
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http://dx.doi.org/10.1016/j.bioactmat.2022.04.030DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9123220PMC
February 2023

Single-molecule nano-optoelectronics: insights from physics.

Rep Prog Phys 2022 Jul 8;85(8). Epub 2022 Jul 8.

Center of Single-Molecule Sciences, Institute of Modern Optics, Frontiers Science Center for New Organic Matter, Tianjin Key Laboratory of Micro-Scale Optical Information Science and Technology, College of Electronic Information and Optical Engineering, Nankai University, 38 Tongyan Road, Jinnan District, Tianjin 300350, People's Republic of China.

Single-molecule optoelectronic devices promise a potential solution for miniaturization and functionalization of silicon-based microelectronic circuits in the future. For decades of its fast development, this field has made significant progress in the synthesis of optoelectronic materials, the fabrication of single-molecule devices and the realization of optoelectronic functions. On the other hand, single-molecule optoelectronic devices offer a reliable platform to investigate the intrinsic physical phenomena and regulation rules of matters at the single-molecule level. To further realize and regulate the optoelectronic functions toward practical applications, it is necessary to clarify the intrinsic physical mechanisms of single-molecule optoelectronic nanodevices. Here, we provide a timely review to survey the physical phenomena and laws involved in single-molecule optoelectronic materials and devices, including charge effects, spin effects, exciton effects, vibronic effects, structural and orbital effects. In particular, we will systematically summarize the basics of molecular optoelectronic materials, and the physical effects and manipulations of single-molecule optoelectronic nanodevices. In addition, fundamentals of single-molecule electronics, which are basic of single-molecule optoelectronics, can also be found in this review. At last, we tend to focus the discussion on the opportunities and challenges arising in the field of single-molecule optoelectronics, and propose further potential breakthroughs.
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http://dx.doi.org/10.1088/1361-6633/ac7401DOI Listing
July 2022

Treatment Strategies in Emergency Endoscopy for Acute Esophageal Variceal Bleeding (CHESS1905): A Nationwide Cohort Study.

Front Med (Lausanne) 2022 27;9:872881. Epub 2022 Apr 27.

Department of Gastroenterology and Endoscopy, The Fifth Affiliated Zhuhai Hospital of Zunyi Medical University, Zhuhai, China.

Background And Aims: Emergency endoscopy is recommended for patients with acute esophageal variceal bleeding (EVB) and their prognosis has improved markedly over past decades due to the increased specialization of endoscopic practice. The study aimed to compare outcomes following emergency endoscopic injection sclerotherapy (EIS) and endoscopic variceal ligation (EVL) in cirrhotic patients with acute EVB.

Methods: Cirrhotic patients with acute EVB who underwent emergency endoscopy were retrospectively enrolled from 2013 to 2020 across 34 university hospitals from 30 cities. The primary outcome was the incidence of 5-day rebleeding after emergency endoscopy. Subgroup analysis was stratified by Child-Pugh class and bleeding history. A 1:1 propensity score matching (PSM) analysis was performed.

Results: A total of 1,017 and 382 patients were included in EIS group and EVL group, respectively. The 5-day rebleeding incidence was similar between EIS group and EVL group (4% vs. 5%, = 0.45). The result remained the same after PSM ( = 1.00). Among Child-Pugh class A, B and C patients, there were no differences in the 5-day rebleeding incidence between the two groups after PSM ( = 0.25, 0.82, and 0.21, respectively). As for the patients with or without bleeding history, the differences between EIS group and EVL group were not significant after PSM ( = 1.00 and 0.26, respectively).

Conclusion: The nationwide cohort study indicates that EIS and EVL are both efficient emergency endoscopic treatment strategies for acute EVB. EIS should not be dismissed as an economical and effective emergency endoscopic treatment strategy of acute EVB. ClincialTrials.gov number NCT04307264.
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http://dx.doi.org/10.3389/fmed.2022.872881DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9092278PMC
April 2022

COVID-19 Experiences and Health-Related Implications: Results From a Mixed-Method Longitudinal Study of Urban Poor Adolescents in Shanghai.

J Adolesc Health 2022 07 7;71(1):30-38. Epub 2022 May 7.

Department of Population, Family and Reproductive Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland; Soins Primaires et Prévention. Inserm U1018, Center for Research in Epidemiology and Population Health (CESP), Villejuif, France.

Purpose: This analysis aimed to investigate gender differences in adolescents' concerns and the health implications of COVID-19.

Methods: We used two rounds of the Global Early Adolescent Study (GEAS) collected in Shanghai in 2018 and 2020. We analyzed data from 621 adolescents, comparing boys' and girls' concerns about COVID-19 and examining trends in general health and mental health by sex between the pre-COVID-19 and COVID-19 periods. Changes in health indicators over time were assessed using generalized estimating equation (GEE) models.

Results: Adolescent girls reported more health concerns (52.0% vs. 42.7%) and educational concerns (61.0% vs. 46.3%) than boys, whereas boys expressed more worries about the economic consequences of COVID-19 (32.9% vs. 25.4%). Changes in health-related outcomes during the pandemic compared to the prepandemic era differed by sex and varied by COVID-related experiences. Boys reported improved overall health (OR: 1.54, 95% CI: 1.00, 2.35) in the COVID-19 period relative to the pre-COVID-19 period. Such improvements were only observed among boys who reported no family economic hardships (OR: 2.10, 95% CI: 1.24, 3.58). We found no significant change for girls (OR: 1.14, 95% CI: 0.83, 1.55), regardless of COVID-19 economic impacts. In contrast, girls reported increased anxiety (OR: 1.63, 95% CI: 1.09, 2.45), especially among those who were concerned about their academic performance (OR: 1.85, 95% CI: 1.16, 2.97). Boys experienced no such increase (OR: 0.92, 95% CI: 0.55, 1.54), regardless of their education concerns.

Discussion: Adolescents' COVID-19 experiences are highly gendered and result in increased health inequalities, with greater mental health implications for girls.
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http://dx.doi.org/10.1016/j.jadohealth.2022.03.016DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9077362PMC
July 2022

Elevated Gamma Connectivity in Nidopallium Caudolaterale of Pigeons during Spatial Path Adjustment.

Animals (Basel) 2022 Apr 14;12(8). Epub 2022 Apr 14.

Henan Key Laboratory of Brain Science and Brain-Computer Interface Technology, School of Electrical Engineering, Zhengzhou University, Zhengzhou 450001, China.

Previous studies showed that spatial navigation depends on a local network including multiple brain regions with strong interactions. However, it is still not fully understood whether and how the neural patterns in avian nidopallium caudolaterale (NCL), which is suggested to play a key role in navigation as a higher cognitive structure, are modulated by the behaviors during spatial navigation, especially involved path adjustment needs. Hence, we examined neural activity in the NCL of pigeons and explored the local field potentials' (LFPs) spectral and functional connectivity patterns in a goal-directed spatial cognitive task with the detour paradigm. We found the pigeons progressively learned to solve the path adjustment task when the learned path was blocked suddenly. Importantly, the behavioral changes during the adjustment were accompanied by the modifications in neural patterns in the NCL. Specifically, the spectral power in lower bands (1-4 Hz and 5-12 Hz) decreased as the pigeons were tested during the adjustment. Meanwhile, an elevated gamma (31-45 Hz and 55-80 Hz) connectivity in the NCL was also detected. These results and the partial least square discriminant analysis (PLS-DA) modeling analysis provide insights into the neural activities in the avian NCL during the spatial path adjustment, contributing to understanding the potential mechanism of avian spatial encoding. This study suggests the important role of the NCL in spatial learning, especially path adjustment in avian navigation.
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http://dx.doi.org/10.3390/ani12081019DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9026408PMC
April 2022

Superior stable, hydrophobic and multifunctional nanocellulose hybrid aerogel via rapid UV induced in-situ polymerization.

Carbohydr Polym 2022 Jul 23;288:119370. Epub 2022 Mar 23.

JihuaGroup Corporation Limited, Beijing, China.

Nanocellulose-based aerogels are potential candidates for the various advanced fields, but the poor structural stability and hydrophily restrains the practical applications. Herein, we report a facile strategy to in-situ polymerizes the polydimethylsiloxane on the anisotropic nanocellulose/chitosan aerogel matrix via UV-induced thiol-ene click reaction in 30 s. A superhydrophobic and elastic nanocellulose-based aerogel with a "stiff-soft" double network structure was fabricated. The inherited porous anisotropic structure and chemical-bonded interlocking interface render the hybrid aerogels with ultralight (9.42 mg/cm), high porosity (99.29%), and hydrophobicity (contact angle of ~168.0°). Moreover, the hybrid aerogel demonstrated robustness (321 kPa), brilliant elasticity (full recovery from 80% compression), outstanding structure stability (>1000 cycles of compression). The reported strategy paves a new way for fabricating high-performance nanocellulose-based aerogel used for thermal insulation and oil absorption in harsh conditions.
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http://dx.doi.org/10.1016/j.carbpol.2022.119370DOI Listing
July 2022

Effective treatment in lung adenocarcinoma patient with brain metastases harboring novel CLHC1/RNT4 intergenic region- ALK fusion: A case report.

Medicine (Baltimore) 2022 Apr 8;101(14):e29134. Epub 2022 Apr 8.

The Medical Department, Jiangsu Simcere Diagnostics Co., Ltd; Nanjing Simcere Medical Laboratory Science Co., Ltd; The State Key Lab of Translational Medicine and Innovative Drug Development, Jiangsu Simcere Diagnostics Co., Ltd, Nanjing, China.

Rationale: Anaplastic lymphoma kinase (ALK) fusion, an important oncogenic mutation, occurs in 3% to 7% of non-small cell lung cancer (NSCLC) cases, and EML4 is the most common partner gene. With the widespread application of next-generation sequencing (NGS), more gene breakpoint fusions have been discovered and functional fusion transcripts can provide targeted clinical benefits.

Patient Concerns And Diagnosis: A 40-year-old woman was diagnosed with lung adenocarcinoma with brain metastases. A novel CLHC1/RNT4 intergenic region, ALK (Exon20-29) (abundance 39.97%), was identified using lung puncture tissue by NGS analysis (Simceredx), and results of immunohistochemistry and fluorescence in situ hybridization confirmed ALK fusion.

Interventions And Outcomes: The patient was administered oral crizotinib (250 mg bid) combined with endostar (30 mg d1-7) for 12 cycles from June 18, 2020. The patient's condition was controlled, and the curative effect was evaluated as stable disease (SD). Unfortunately, brain magnetic resonance images showed multiple nodules in the left cerebellar hemisphere, and chest computed tomography showed no significant changes in the progression of the disease. Subsequently, alectinib (600 mg bid) was administered on April 1, 2021. Brain lesions were significantly reduced and partial remission (PR) was achieved. No significant changes were observed in the lung lesions.

Lessons: ALK fusion is a risk factor for brain metastasis (BM) in patients with advanced non-small NSCLC patients. In our case, a novel CLHC1/RNT4 intergenic region, ALK fusion, was identified for the first time in a lung adenocarcinoma patient with BM, who benefited from crizotinib and endostar sequential alectinib. Our case highlights the advantages of NGS for fusion detection and provides promising treatment options for NSCLC patients with BM harboring ALK fusions.
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http://dx.doi.org/10.1097/MD.0000000000029134DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9276468PMC
April 2022

Reliability and validity of the Chinese version of the Family Resilience Questionnaire (FaRE Questionnaire) in patients with breast cancer: a cross-sectional study.

BMJ Open 2022 04 20;12(4):e051093. Epub 2022 Apr 20.

Nursing department, Zhengzhou Central Hospital Affiliated To Zhengzhou University, Zhengzhou, China

Objective: The aim of this study was to assess the reliability and validity of the Chinese version of the Family Resilience (FaRE) Questionnaire among patients with breast cancer in China.

Design: It was a cross-sectional study, which involved translation, back-translation, cultural adjustment and psychometric testing of a 24-item FaRE Questionnaire.

Setting: Three tertiary hospitals in Zhengzhou, China: respectively are the First Affiliated Hospital of Zhengzhou University, Second Hospital Affiliated to Zhengzhou University and Henan Provincial People's hospital.

Participants: A total of 559 patients with breast cancer volunteered to participate in the study PRIMARY OUTCOME MEASURES: Data analysis was performed using the IBM SPSS software V.21.0 and AMOS software V.21.0. Cronbach's α coefficient was used to examine the internal consistency. The test-retest reliability was calculated using the intraclass correlation coefficient on 30 participants. The content validity index was calculated based on the values obtained from six expert opinions. Construct validity test was performed using factor analysis including exploratory factor analysis and confirmatory factor analysis.

Results: For the Chinese version of FaRE Questionnaire, the Cronbach's α coefficient of the total questionnaire was 0.909, and Cronbach's α coefficients of four factors were 0.902, 0.932, 0.905 and 0.963, respectively. The test-retest reliability index of the total questionnaire was 0.905. The Scale-Content Validity Index was 0.97, and Item-Content Validity Index ranged from 0.83 to 1.00. The questionnaire included 24 items, exploratory factor analysis extracted four factors with loading >0.4, which could explain 72.146% of the total variance. Confirmatory factor analysis showed the Chinese version of FaRE Questionnaire had an excellent four-factor model consistent with the original questionnaire.

Conclusion: The Chinese version of FaRE Questionnaire has acceptable reliability and validity among patients with breast cancer in China. It can effectively assess family resilience and provide basis for personalised family resilience interventions for patients with breast cancer.
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http://dx.doi.org/10.1136/bmjopen-2021-051093DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9021805PMC
April 2022
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