Publications by authors named "Menglin Wang"

81 Publications

Acupuncture for chronic persistent asthma: a case report.

Acupunct Med 2021 Sep 19:9645284211040086. Epub 2021 Sep 19.

Acupuncture and Tuina School, Chengdu University of Traditional Chinese Medicine, Chengdu, China.

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http://dx.doi.org/10.1177/09645284211040086DOI Listing
September 2021

The anti-fibrotic drug pirfenidone inhibits liver fibrosis by targeting the small oxidoreductase glutaredoxin-1.

Sci Adv 2021 Sep 1;7(36):eabg9241. Epub 2021 Sep 1.

Center for Pharmacogenetics and Department of Pharmaceutical Sciences, University of Pittsburgh, Pittsburgh, PA 15261, USA.

[Figure: see text].
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http://dx.doi.org/10.1126/sciadv.abg9241DOI Listing
September 2021

[Effects of decabromodiphenyl ether on glucose and lipid metabolism and adipocyte factors in C57BL/6 mice].

Wei Sheng Yan Jiu 2021 May;50(3):476-482

School of Public Health, Xinjiang Medical University, Urumqi 830011, China.

Objective: To investigate the effect of decabromodiphenyl ether(BDE-209) on glucose and lipid metabolism and adipocytokines in mice.

Methods: A total of 36 adult male C57 BL/6 mice were randomly divided into 6 groups, 1000, 800, 600, 450 and 300 mg/kg groups and the control group, with 6 in each group. 60 days after gavage, fasting overnight, the mice were killed and the corresponding test materials were taken. Using an automatic biochemical analyzer to detect triglycerides(TG), total cholesterol(cholesterol, TC), low-density lipoprotein(LDL-C), and high-density lipoprotein(HDL-C). Detected rat fasting blood glucose(FBG) with Roche blood glucose meter. Observed the morphological changes of mouse liver tissue using HE staining. Determined leptin(LEP), the mRNA and protein expression levels of adiponectin(ADP) and peroxisome proliferators-activated receptors(PPAR)-γ with Real-time fluorescent quantitative PCR and Western blotting.

Results: The levels of fasting serum lisulin(FINS), insulin sensitivity index(ISI), ADP and HDL-C in the serum of the BDE-209 exposure group decreased(P& lt; 0. 05). The levels of FBG, LEP, TC, TG and LDL-C in the serum of the BDE-209 exposure group increased(P& lt; 0. 05). mRNA and protein expression levels of LEP and PPAR-γ in the liver increased(P& lt; 0. 05), and ADP mRNA and protein expression levels decreased(P& lt; 0. 05). Adipocyte factor LEP was positively correlated with the content of FBG, TG, TC and LDL-C, with r values of 0. 775, 0. 767, 0. 716 and 0. 812(P& lt; 0. 05), and negatively correlated with FINS and HDL-C, with r values of-0. 919 and-0. 817(P& lt; 0. 05). ADP was positively correlated with FINS and HDL-C, with r values of 0. 824 and 0. 832(P& lt; 0. 05), and negatively correlated with FBG, TG, TC and LDL-C, with r values of-0. 883, -0. 686, -0. 704 and-0. 772, respectively(P& lt; 0. 05). BDE-209 exposure to each dose group could change the morphology of mouse liver tissue to a certain extent.

Conclusion: Decabromodiphenyl ether can disturb the metabolism of glucose and lipids. Among them, the disorder in the high and high dose groups of BDE-209 is more obvious. The low dose of BDE-209 can also change the morphology of liver tissue.
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http://dx.doi.org/10.19813/j.cnki.weishengyanjiu.2021.03.022DOI Listing
May 2021

LCT-3d Induces Oxidative Stress-Mediated Apoptosis by Upregulating Death Receptor 5 in Gastric Cancer Cells.

Front Oncol 2021 16;11:658608. Epub 2021 Apr 16.

Key Laboratory of Advanced Technology for Drug Preparation, Ministry of Education, School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, China.

Gastric cancer is a global health problem. In this study, we investigate the role of a novel Indole derivative, named LCT-3d, in inhibiting the growth of gastric cancer cells by MTT assay. The Western blotting results showed that LCT-3d modulated the mitochondrial-related proteins and Cleaved-Caspases 3/9, to induce cell apoptosis. The up-regulation of Death receptor 5 (DR5) in MGC803 cells was observed with LCT-3d treatment. Knockdown of DR5 on MGC803 cells partially reversed the LCT-3d-induced mitochondrial apoptosis. The level of Reactive Oxygen Species (ROS) in MGC803 cells was increased with LCT-3d treatment and could be blocked with the pretreatment of the ROS inhibitor N-Acetylcysteine (NAC). The results demonstrate that the elevating ROS can up-regulate the expression of DR5, resulting in apoptosis mitochondrial pathway. Although the nuclear factor erythroid-2 related factor 2 (Nrf2) pathway served an important role in protecting gastric cancer cells against the injury of ROS, it can't reverse LCT-3d-induced cell apoptosis. Taken together, our study showed that LCT-3d induced apoptosis DR5-mediated mitochondrial apoptotic pathway in gastric cancer cells. LCT-3d could be a novel lead compound for development of anti-cancer activity in gastric cancer.
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http://dx.doi.org/10.3389/fonc.2021.658608DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8085419PMC
April 2021

Participation of the Anterior Cingulate Cortex in Sodium Salicylate-induced Tinnitus.

Otol Neurotol 2021 Sep;42(8):e1134-e1142

Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, The Chinese Academy of Sciences, Beijing, China.

Hypothesis: The anterior cingulate cortex (ACC) participates in sodium salicylate (SS)-induced tinnitus through alteration of the disordered neural activity and modulates the neuronal changes in the auditory cortex (AC).

Background: Although the mechanism underlying tinnitus remains unclear, the crucial roles of the auditory center and limbic system in this process have been elucidated. Recent reports suggest that dysfunction of the ACC, an important component of the limbic system that regulates and controls the conduction of multiple sensations, is involved in tinnitus. Although altered functional connectivity between the ACC and the auditory system has been observed in humans with tinnitus, the underlying neuronal mechanism remains unexplored.

Methods: SS (350 mg/kg, 10%, i.p.) was used to yield tinnitus model in rats, followed by comparison of the alteration in the spontaneous firing rate (SFR), local field potential (LFP), and extracellular glutamic acid in the ACC. The responses of neurons in the AC to electrical stimulation from the ACC were also observed.

Results: We determined significant increases in the neuronal SFR and extracellular glutamate level in the ACC after SS injection (p < 0.05). These effects were accompanied by decreased alpha band activity and increased beta and gamma band activity (p < 0.05). In the majority of AC neurons, the SFR decreased in response to ACC stimulation (p < 0.05).

Conclusions: Our results demonstrated that disordered neural activity in the ACC contributes to SS-induced tinnitus and that ACC activation can modulate AC activity.
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http://dx.doi.org/10.1097/MAO.0000000000003183DOI Listing
September 2021

The Altered Functional Connectivity With Pain Features Integration and Interaction in Migraine Without Aura.

Front Neurosci 2021 4;15:646538. Epub 2021 Mar 4.

Acupuncture and Tuina School/The 3rd Teaching Hospital, Chengdu University of Traditional Chinese Medicine, Chengdu, China.

Introduction: Migraine without aura (MwoA) is a primary type of migraine, a common disabling disorder, and a disabling neurological condition. The headache is a complex experience, a common form of pain, in which multiple sensory information dimensions are combined to provide a unified conscious event. Migraine ictal have unique neuroimage biomarkers, but the brain is also affected during the inter-ictal phase. According to the current studies, a hypothesis was constructed that the altered integration of pain spatial and intensity information impacts headache intensity in the inter-ictal period.

Methods: In this study, we applied theory-based region-to-region functional connectivity (FC) analyses to compare the differences in resting-state FC between MwoA participants and healthy controls with the pain integration hypothesis. After the correlation matrices between FC edges and clinical symptoms were constructed, the moderating effect and simple slope tests were investigated to explain whether and how the dysfunction of pain features discrimination affects the clinical symptoms.

Results: Functional connectivity analyses showed significantly decreased FC edges between the left dorsolateral superior frontal gyrus (SFGdor) and left insula, and an increased FC edge between the left SFGdor and bilateral angular gyrus. The correlation matrix showed no significant correlation between significantly altered FC edge and headache duration, frequency, Zung self-rating anxiety scale, and Zung self-rating depression scale. Only one significantly altered edge in the MwoA condition was significantly correlated with headache intensity. Moderating Module 1 and 2 manifested the moderator variable (altered rs-FC edge) moderated the link between the normal edges and headache intensity.

Conclusion: The pain features integration processes in migraineurs vary from HCs, related to the clinical symptoms during a migraine attack. Moreover, the clinical symptoms will be affected by one or more discrimination modules. And the spatial or intensity discrimination modules have a higher impact when combined with another module on clinical symptoms than the single module.
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http://dx.doi.org/10.3389/fnins.2021.646538DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7969893PMC
March 2021

Application of a six sigma model to the evaluation of the analytical performance of serum enzyme assays and the design of a quality control strategy for these assays: A multicentre study.

Clin Biochem 2021 May 20;91:52-58. Epub 2021 Feb 20.

Department of Laboratory Medicine, The Second People's Hospital of Lianyungang, Lianyungang, PR China. Electronic address:

Background: Six medical testing laboratories at six different sites in China participated in this study. We applied a six sigma model for (a) the evaluation of the analytical performance of serum enzyme assays at each of the laboratories, (b) the design of individualized quality control programs and (c) the development of improvement measures for each of the assays, as appropriate.

Methods: Internal quality control (IQC) and external quality assessment (EQA) data for selected serum enzyme assays were collected from each of the laboratories. Sigma values for these assays were calculated using coefficients of variation, bias, and total allowable error (TEa). Normalized sigma method decision charts were generated using these parameters. IQC design and improvement measures were defined using the Westgard sigma rules. The quality goal index (QGI) was used to assist with identification of deficiencies (bias problems, precision problems, or their combination) affecting the analytical performance of assays with sigma values <6.

Results: Sigma values for the selected serum enzyme assays were significantly different at different levels of enzyme activity. Differences in assay quality in different laboratories were also seen, despite the use of identical testing instruments and reagents. Based on the six sigma data, individualized quality control programs were outlined for each assay with sigma <6 at each laboratory.

Conclusions: In multi-location laboratory systems, a six sigma model can evaluate the quality of the assays being performed, allowing management to design individualized IQC programs and strategies for continuous improvement as appropriate for each laboratory. This will improve patient care, especially for patients transferred between sites within multi-hospital systems.
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http://dx.doi.org/10.1016/j.clinbiochem.2021.02.004DOI Listing
May 2021

Relaxin gene delivery modulates macrophages to resolve cancer fibrosis and synergizes with immune checkpoint blockade therapy.

Sci Adv 2021 Feb 17;7(8). Epub 2021 Feb 17.

Division of Pharmacoengineering and Molecular Pharmaceutics, Eshelman School of Pharmacy, University of North Carolina, Chapel Hill, NC 27599, USA.

Cancer fibrosis serves as a major therapeutic barrier in desmoplastic tumors. Relaxin (RLN; a systemic hormone) is efficacious to decrease fibrosis, but the in vivo mechanism of action is not clear. Considering the localization of relaxin family peptide receptor type 1 (RXFP1), the receptor for RLN, on macrophages, we hypothesize that macrophages can be modulated by RLN to ameliorate cancer fibrosis. Using KPC mouse model of pancreatic ductal adenocarcinoma (PDAC), here, we report locally expressed RLN with targeted gene delivery induces increased F4/80CD206 macrophages originating from Ly6C monocytes, promoting fibrosis depletion and cytotoxic T cell infiltration. Moreover, RLN gene delivery synergizes with PD-L1 blockade for tumor inhibition by enhancing T cell-mediated tumor cell killing and macrophage phagocytosis. Collectively, our results reveal previously unidentified insights into the modulation of macrophages to regulate tumor-associated fibrosis, providing a feasible strategy to reverse the immunosuppressive environment and improve the therapeutic outcome of checkpoint immunotherapies.
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http://dx.doi.org/10.1126/sciadv.abb6596DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7888957PMC
February 2021

Tumor-targeted gene therapy with lipid nanoparticles inhibits tumor-associated adipocytes and remodels the immunosuppressive tumor microenvironment in triple-negative breast cancer.

Nanoscale Horiz 2021 04 15;6(4):319-329. Epub 2021 Feb 15.

Division of Pharmacoengineering and Molecular Pharmaceutics and Center for Nanotechnology in Drug Delivery, Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.

Adipocytes are the primary cellular components within the tumor microenvironment (TME) of triple-negative breast cancer (TNBC). Increasing evidence suggests that tumor-associated adipocytes (TAAs) can aggravate tumor progression, exacerbate the immunosuppressive TME and compromise therapeutic efficacy. In this study, the biological effect of TAAs within the breast cancer TME is first investigated, and the C-C Motif Chemokine Ligand 2 (CCL2) which is mainly secreted by TAAs in the extracellular environment is identified as the key mediator. CCL2 recruits immune cells such as monocytes and macrophages that further differentiated into immunosuppressive myeloid-derived suppressor cells (MDSCs) and M2 macrophages. To manipulate CCL2-mediated immune response, a protein trap that binds with CCL2 with high affinity and specificity is designed. The plasmid DNA encoding the CCL2 trap (pCCL2) is specifically delivered to the TME by using targeted lipid-protamine-DNA (LPD) nanoparticles to locally express the CCL2 trap and ameliorate the immunosuppressive TME. Significantly, compared with the commercially available CCL2 antibody, this strategy shows enhanced therapeutic efficacy and appreciable tumor growth inhibition. Furthermore, the pCCL2 trap treatment successfully suppresses TAAs, increases T cell infiltration and decreases the population of immunosuppressive M2 macrophages and MDSCs. Further studies show that the pCCL2 trap could facilitate PD-L1 blockade immunotherapy, demonstrating its translation potential.
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http://dx.doi.org/10.1039/d0nh00588fDOI Listing
April 2021

Label-Free Resistance Cytometry at the Orifice of a Nanopipette.

Anal Chem 2021 02 27;93(5):2942-2949. Epub 2021 Jan 27.

Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.

Development of new principles and techniques at the single-cell level is significantly important since cells as basic units of living organisms always bear large heterogeneity. Herein, we demonstrate a new electrochemical principle for single-cell analysis based on an ion current blockage at the orifice of a nanopipette, defined as resistance cytometry. The amplitude and the frequency of ion current transients show strong dependence on the size and the concentration of cells, which could be used for in situ cell sizing and counting. This technique shows good ability to detect the size change of RBCs under stimulations of different pH and osmotic pressure values. More importantly, the as-presented resistance cytometry can distinguish lymphoma blood cells from normal blood cells for patient blood samples. The as-presented resistance cytometry is label-free, non-invasive, and non-destructive, which not only opens new opportunities for single-cell analysis but also provides a new platform for cell-related medical diagnostic technologies.
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http://dx.doi.org/10.1021/acs.analchem.0c04585DOI Listing
February 2021

Hepatic macrophages act as a central hub for relaxin-mediated alleviation of liver fibrosis.

Nat Nanotechnol 2021 04 25;16(4):466-477. Epub 2021 Jan 25.

Division of Pharmacoengineering and Molecular Pharmaceutics, Eshelman School of Pharmacy, University of North Carolina, Chapel Hill, NC, USA.

Relaxin is an antifibrotic peptide hormone previously assumed to directly reverse the activation of hepatic stellate cells for liver fibrosis resolution. Using nanoparticle-mediated delivery, here we show that, although relaxin gene therapy reduces liver fibrosis in vivo, in vitro treatment fails to induce quiescence of the activated hepatic stellate cells. We show that hepatic macrophages express the primary relaxin receptor, and that, on relaxin binding, they switch from the profibrogenic to the pro-resolution phenotype. The latter releases exosomes that promote the relaxin-mediated quiescence of activated hepatic stellate cells through miR-30a-5p. Building on these results, we developed lipid nanoparticles that preferentially target activated hepatic stellate cells in the fibrotic liver and encapsulate the relaxin gene and miR-30a-5p mimic. The combinatorial gene therapy achieves synergistic antifibrosis effects in models of mouse liver fibrosis. Collectively, our findings highlight the key role that macrophages play in the relaxin-primed alleviation of liver fibrosis and demonstrate a proof-of-concept approach to devise antifibrotic strategies through the complementary application of nanotechnology and basic science.
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http://dx.doi.org/10.1038/s41565-020-00836-6DOI Listing
April 2021

Heavy Metal Exposure Leads to Rapid Changes in Cellular Biophysical Properties.

ACS Biomater Sci Eng 2020 04 24;6(4):1965-1976. Epub 2020 Mar 24.

School of Environment and Energy, South China University of Technology, Guangzhou 510006, China.

Biophysical properties of cells, such as cell mechanics, cell shape, and cell migration, are emerging hallmarks for characterizing various cell functions. Conversely, disruptions to these biophysical properties may be used as reliable indicators of disruptions to cell homeostasis, such as in the case of chemical-induced toxicity. In this study, we demonstrate that treatment of lead(II) nitrate and cadmium nitrate leads to dosage-dependent changes in a collection of biophysical properties, including cellular traction forces, focal adhesions, mechanical stiffness, cell shape, migration speed, permeability, and wound-healing efficacy in mammalian cells. As those changes appear within a few hours after the treatment with a trace amount of lead/cadmium, our results highlight the promise of using biophysical properties to screen environmental chemicals to identify potential toxicants and establish dose response curves. Our systematic and quantitative characterization of the rapid changes in cytoskeletal structure and cell functions upon heavy metal treatment may inspire new research on the mechanisms of toxicity.
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http://dx.doi.org/10.1021/acsbiomaterials.9b01640DOI Listing
April 2020

Acupuncture for chronic persistent asthma based on the theory of Meridian-viscera Association: study protocol for a multi-center randomized controlled trial in China.

Trials 2021 Jan 6;22(1):17. Epub 2021 Jan 6.

Acupuncture and Tuina School/The 3rd Teaching Hospital, Chengdu University of Traditional Chinese Medicine, 37# Shierqiao Road, Chengdu, 610075, Sichuan, China.

Background: Acupuncture is effective in symptom and quality of life improvement of chronic asthma, but the efficacy differences between different acupoints are uncertain. In terms of the theory of Meridian-viscera Association, the study aims to investigate the different effectiveness between acupoints in Lung meridian and the acupoints in Heart meridian, so as to provide the evidence to develop a better prescription of the acupuncture treatment of chronic persistent asthma.

Methods: This study is a multicentral randomized controlled trial. A total of 68 chronic persistent asthma patients will be randomly allocated into two groups: the Lung meridian group and the Heart meridian group. This trial will include a 2-week baseline period, a 4-week treatment period with 12 sessions' acupuncture, and an 8-week follow-up period. The primary outcome is the Asthma Quality of Life Questionnaire (AQLQ). Secondary outcomes are the Asthma Control Test (ACT), Peak Expiratory Flow (PEF), and Forced Expiratory Volume in 1 s (FEV1). The AQLQ and ACT will be collected at baseline, week 4, week 8, and week 12 after randomization. PEF, FEV1, the Self-rating Anxiety Scale (SAS), and the Self-rating Depression Scale (SDS) will be assessed at baseline and week 4.

Discussion: The results will provide evidence for acupuncture prescription selection and the clinical efficacy improvement. The results of this trial will also be used to determine whether or not a full definitive trial will go ahead, which will further confirm the theory of Meridian-viscera Association.

Trial Registration: Chinese Clinical Trial Registry ( http://www.chictr.org.cn/showproj.aspx?proj=43803 ) ChiCTR1900027284. Registered on 7 November 2019.
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http://dx.doi.org/10.1186/s13063-020-04844-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7789474PMC
January 2021

Vaccine of RANKL mutant conjugated with KLH effectively stabilizing bone metabolism and preventing trabecular microstructural degeneration in osteoporotic rats.

J Orthop Res 2020 Dec 31. Epub 2020 Dec 31.

Department of Orthopedics, Beijing Shijitan Hospital, Capital Medical University, Beijing, China.

Receptor activator of nuclear factor kappa-B ligand (RANKL) is one of the key factors regulating the maturation of osteoclasts and an important target for osteoporosis treatment. A monoclonal antibody against RANKL showed effective therapeutic activity against osteoporosis by inhibiting bone resorption by osteoclasts. However, being an exogenous protein, its efficacy decreases after long-term use, and its discontinuation increases the risk of vertebral fractures. Here, we aimed to design an active immunotherapeutic agent to induce a T-cell dependent primary response. The agent, a mutant RANKL vaccine (mRv), was produced by cross-linking mutant RANKL, lacking the ability to stimulate osteoclast maturation, with the carrier protein keyhole limpet hemocyanin, a neo-antigen with a large molecular mass. Subcutaneous injection of mRv stimulated rats with ovariectomy-induced osteoporosis to produce high titers of anti-RANKL antibodies. The mutant RANKL vaccine decreased serum CTX-1 and BALP levels and inhibited the microstructural degeneration of trabecular bone in osteoporotic rats. mRv overcame immune system tolerance, stimulated rats to produce therapeutic antibodies, stabilized bone metabolism, and inhibited trabecular microstructural degeneration. These findings confirm the potential of the mutant RANKL vaccine to be developed into an effective preventive and therapeutic agent for osteoporosis.
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http://dx.doi.org/10.1002/jor.24980DOI Listing
December 2020

Parahiraciini (Hemiptera, Fulgoromorpha, Issidae): one new genus, two new species and three new subtribes.

Zookeys 2020 25;997:69-94. Epub 2020 Nov 25.

Key Laboratory of Southwest China Wildlife Resources Conservation of the Ministry of Education, China West Normal University, Nanchong, Sichuan Province, 637009, China China West Normal University Nanchong China.

A new genus with two new species and are described respectively from Vietnam and China in the tribe Parahiraciini of the family Issidae. A molecular phylogeny using combined genes (18S, 28S, COX1 and Cytb) confirms its placement in the tribe Parahiraciini. The tribe is divided into three subtribes: Scantiniina with the type genus Stål, 1866, and Vindilisina with type genus Stål, 1870 plus Gnezdilov, 2017, and the nominal subtribe Parahiraciina Cheng & Yang, 1991 The characteristics of these subtribes are provided, with a key to identification. Genus Che, Zhang & Wang, 2013 is transferred to HemisphaeriiniMongolianina, and genus Yang, 1994 is maintained as a valid name.
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http://dx.doi.org/10.3897/zookeys.997.52857DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7710678PMC
November 2020

A New Type of [email protected] Nanocarrier for Magnetic Targeting and Light-Microwave Dual Controlled Drug Release.

Int J Nanomedicine 2020 10;15:8783-8802. Epub 2020 Nov 10.

Hunan Provincial Key Laboratory of Fine Ceramics and Powder Materials, Hunan University of Humanities, Science and Technology, Lou'di, Hunan, People's Republic of China.

Introduction: Cancer is a major health problem worldwide, and the most extensive treatment can be obtained by using chemotherapy in the clinic. However, due to the low selectivity of cancer cells, chemotherapy drugs produce a series of grievous side effects on normal cells.

Methods: In this research, we developed novel nanocarriers for magnetically targeted near-infrared (NIR) light-electromagnetic wave dual controlled drug delivery based on [email protected] nanoparticles (NPs) modified with aminopropyltriethoxysilane (APTES) in response to magnetic, NIR light, and electromagnetic wave irradiation. Synthesis and characterization of [email protected] NPs was carried out using X-ray diffraction measurements, scanning electron microscopy, transmission electron microscopy, photoluminescence emission spectra, UV-1800 spectrophotometer, N5230A vector network analyzer, MDS-6 microwave sample preparation system, and superconducting quantum interference device. In addition to that mentioned above, we also explored many other sides, such as the drug-loading, drug-controlled release efficiency, elect99omagnetic wave thermal effect and photo-thermal effect.

Results: The results showed that APTES-modified [email protected] NPs had 37% high drug-loading capacity and high electromagnetic wave thermal conversion ability and NIR-light thermal conversion ability. In addition, ibuprofen (IBU) release from the [email protected] depends on the electromagnetic wave (2.45 GHz) and 1060 nm NIR light irradiation. After five cycles, the drug-release percentage was 90% and 66% separately, and could be adjusted by the time and cycles times of electromagnetic wave and NIR light irritation. Electromagnetic wave irradiation compared with NIR light irradiation, has a higher drug release rate and better penetration. Therefore, choosing different stimulation methods according to the treatment needs of the disease, we can achieve accurate personalized treatment of the disease.

Discussion: Our findings indicate that multifunctional APTES modified [email protected] NPs could be used for the first time as a new drug carrier for "location-timing-quantification" drug release with magnetic targeting and dual control of NIR light-electromagnetic waves.
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http://dx.doi.org/10.2147/IJN.S267614DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7667186PMC
December 2020

Notes on genera of Sarimini with description of two new species in the genera Microsarimodes and Tetrichina (Hemiptera: Fulgoromorpha: Issidae).

Zootaxa 2020 Oct 2;4858(4):zootaxa.4858.4.7. Epub 2020 Oct 2.

Key Laboratory of Plant Protection Resources and Pest Management of the Ministry of Education, Entomological Museum, Northwest AF University, Yangling, Shaanxi, 712100, China Key Laboratory of Southwest China Wildlife Resources Conservation of the Ministry of Education, China West Normal University, Nanchong, Sichuan Province, 637009, China..

Two new species of the tribe Sarimini in the planthopper family Issidae: Microsarimodes flavomaculata sp. nov. and Tetrichina fuscovinclum sp. nov. are described from China. The genera Duplexissus Wang, Zhang Bourgoin, 2019 and Sarimissus Wang, Zhang Bourgoin, 2019 are reestablished as valid genera based on both morphological grounds and molecular analyses.
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http://dx.doi.org/10.11646/zootaxa.4858.4.7DOI Listing
October 2020

A Circle RNA Regulatory Axis Promotes Lung Squamous Metastasis via CDR1-Mediated Regulation of Golgi Trafficking.

Cancer Res 2020 11 25;80(22):4972-4985. Epub 2020 Sep 25.

Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina.

Lung squamous carcinoma (LUSC) is a highly metastatic disease with a poor prognosis. Using an integrated screening approach, we found that miR-671-5p reduces LUSC metastasis by inhibiting a circular RNA (circRNA), CDR1as. Although the putative function of circRNA is through miRNA sponging, we found that miR-671-5p more potently silenced an axis of CDR1as and its antisense transcript, cerebellar degeneration related protein 1 (CDR1). Silencing of CDR1as or CDR1 significantly inhibited LUSC metastases and CDR1 was sufficient to promote migration and metastases. CDR1, which directly interacted with adaptor protein 1 (AP1) complex subunits and coatomer protein I (COPI) proteins, no longer promoted migration upon blockade of Golgi trafficking. Therapeutic inhibition of the CDR1as/CDR1 axis with miR-671-5p mimics reduced metastasis . This report demonstrates a novel role for CDR1 in promoting metastasis and Golgi trafficking. These findings reveal an miRNA/circRNA axis that regulates LUSC metastases through a previously unstudied protein, CDR1. SIGNIFICANCE: This study shows that circRNA, CDR1as, promotes lung squamous migration, metastasis, and Golgi trafficking through its complimentary transcript, CDR1.
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http://dx.doi.org/10.1158/0008-5472.CAN-20-1162DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7669576PMC
November 2020

A low toxic CRM1 degrader: Synthesis and anti-proliferation on MGC803 and HGC27.

Eur J Med Chem 2020 Nov 7;206:112708. Epub 2020 Aug 7.

Key Laboratory of Advanced Technology for Drug Preparation, Ministry of Education, School of Pharmaceutical Sciences, Zhengzhou University, 100 Kexue Avenue, Zhengzhou, 450001, China; Collaborative Innovation Center of New Drug Research and Safety Evaluation, Henan Province, Zhengzhou University, No. 100, KeXueDaDao, Zhengzhou, 450001, China; State Key Laboratory of Esophageal Cancer Prevention & Treatment, Zhengzhou University, Zhengzhou, Henan Province, 450052, PR China. Electronic address:

Chromosome region maintenance 1 (CRM1) is the sole nuclear exporter of several tumor suppressor, a growth regulatory protein as an attractive cancer drug target. In the present work, a novel CRM1 degrader was discovered from newly synthesized α, β-unsaturated-δ-lactone based on a natural product Goniothalamin. It induces apoptosis of both MGC803 and HGC27 cell lines via degrading CRM1. Selective inhibition was observed for the proliferation of gastric cancer cell lines MGC803, HGC27 comparing to Human Gastric Mucosal Epithelial Cell Line (GES1). For the first time, CRM1 inhibitor or degrader inducing apoptosis in gastric carcinoma was investigated.
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http://dx.doi.org/10.1016/j.ejmech.2020.112708DOI Listing
November 2020

Surface Iron Species in Palladium-Iron Intermetallic Nanocrystals that Promote and Stabilize CO Methanation.

Angew Chem Int Ed Engl 2020 Aug 11;59(34):14434-14442. Epub 2020 May 11.

Hefei National Laboratory for Physical Sciences at the Microscale, National Synchrotron Radiation Laboratory, CAS Key Laboratory of Strongly-Coupled Quantum Matter Physics, Key Laboratory of Surface and Interface Chemistry and Energy Catalysis of Anhui Higher Education Institutes, Department of Chemical Physics, University of Science and Technology of China, Hefei, Anhui, 230026, P. R. China.

It is of pivotal importance to develop efficient catalysts and investigate the intrinsic mechanism for CO methanation. Now, it is reported that PdFe intermetallic nanocrystals afforded high activity and stability for CO methanation. The mass activity of fct-PdFe nanocrystals reached 5.3 mmol g  h , under 1 bar (CO :H =1:4) at 180 °C, being 6.6, 1.6, 3.3, and 5.3 times as high as that of fcc-PdFe nanocrystals, Ru/C, Ni/C, and Pd/C, respectively. After 20 rounds of successive reaction, 98 % of the original activity was retained for PdFe intermetallic nanocrystals. Further mechanistic studies revealed that PdFe intermetallic nanocrystals enabled the maintenance of metallic Fe species via a reversible oxidation-reduction process in CO methanation. The metallic Fe in PdFe intermetallic nanocrystals induced the direct conversion of CO into CO* as the intermediate, contributing to the enhanced activity.
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http://dx.doi.org/10.1002/anie.201916032DOI Listing
August 2020

On the tribe Sarimini with two new genera from south of China (Hemiptera, Fulgoromorpha, Issidae).

Zootaxa 2019 Dec 9;4706(2):zootaxa.4706.2.10. Epub 2019 Dec 9.

Key Laboratory of Plant Protection Resources and Pest Management of the Ministry of Education, Entomological Museum, Northwest AF University, Yangling, Shaanxi, 712100, China Key Laboratory of Southwest China Wildlife Resources Conservation of the Ministry of Education, China West Normal University, Nanchong, Sichuan, 637009, China.

Two new genera of the tribe Sarimini are described from south of China. They are Sarimissus gen. nov. with a new species S. maculifrons sp. nov. from Hainan province and Duplexissus gen. nov. with a new species D. punctatulus sp. nov. from Yunnan province. The photos of habitus and the male genitalia of the new species are provided. The biology, distribution and origin of the tribe Sarimini are discussed.
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http://dx.doi.org/10.11646/zootaxa.4706.2.10DOI Listing
December 2019

Nanosponges of circulating tumor-derived exosomes for breast cancer metastasis inhibition.

Biomaterials 2020 Mar 4;242:119932. Epub 2020 Mar 4.

Department of Pharmaceutics, Wuya College of Innovation, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, Liaoning, 110016, PR China. Electronic address:

Breast cancer contributes to high mortality rates as a result of metastasis. Tumor-derived exosomes facilitate the development of the premetastatic environment, interacting and inhibiting the normal function of immune cells, thereby forming an immunosuppressive microenvironment for tumor metastasis. Herein, the platelet and neutrophil hybrid cell membrane (PNM) was embellished on a gold nanocage (AuNC) surface called nanosponges and nanokillers (NSKs). NSKs can simultaneously capture and clear the circulating tumor cells (CTCs) and tumor-derived exosomes via high-affinity membrane adhesion receptors, effectively cutting off the connection between exosomes and immune cells. Bionic NSK is loaded with doxorubicin (DOX) and indocyanine green (ICG) for synergic chemo-photothermal therapy. NSKs show greater cellular uptake, deeper tumor penetration, and higher cytotoxicity to tumor cells in comparison to non-coated AuNCs or single-coated AuNCs in vitro. In vivo, the multipurpose NSKs could not only completely ablate the primary tumor but also inhibit breast cancer metastasis with high efficiency in xenograft and orthotopic breast tumor-bearing models. Thus, NSKs could be a promising nanomedicine for the future clinical intervention of breast cancer metastasis.
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http://dx.doi.org/10.1016/j.biomaterials.2020.119932DOI Listing
March 2020

Finite element analysis of different screw constructs in the treatment of unstable femoral neck fractures.

Injury 2020 Apr 17;51(4):995-1003. Epub 2020 Feb 17.

Department of Orthopaedics, The Third People's Hospital of Qingdao, No. 29 Yongping Road, Qingdao 266041, PR China. Electronic address:

Objectives: In the present study, we evaluated the mechanical outcome of different configurations formed by partially threaded screws (PTS) alone or combined screws consisting of PTS and fully threaded screws (FTS) in the treatment of unstable femoral neck fracture.

Methods: The Pauwels type III unstable femoral fracture and screw models of PTS and FTS were created in 3-matic software and UG-NX software respectively. We assembled the different screw fixation types to the fracture model separately to form the fixation models. We used Abaqus software to perform the finite element analysis.

Results: Our results indicated that the peak von Mises stresses of screws increased when some PTSs changed into FTSs in all groups except for the inferior triangle group. FTS in each group underwent the most stress while PTS underwent a little bit of stress. The combined screws fixation types were less likely to be cut-out and was more stable than PTPs alone fixation strategy. Less yielding regions around the screw tunnels for the superior and inferior triangle configuration fixed by combined screws was indicated. Superior triangle fixation model underwent the largest area of stress concentration around the screw holes after screws removal.

Conclusions: For unstable femoral neck fractures, superior results were obtained by stabilizing the fracture with superior or inferior triangle configuration consisting of one PTS and two FTSs. If screws removal was taken into account after fracture union, fixation type of inferior triangle configuration should be the recommended choice.
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http://dx.doi.org/10.1016/j.injury.2020.02.075DOI Listing
April 2020

A new genus of the tribe Sarimini (Fulgoromorpha, Issidae) from the Guangxi Province of China.

Zookeys 2020 17;912:13-23. Epub 2020 Feb 17.

Institut de Systématique, Évolution, Biodiversité, ISYEB-UMR 7205 MNHN-CNRS-Sorbonne Université-EPHE, Muséum national d'Histoire naturelle, CP 50, 57 rue Cuvier, F-75005 Paris, France Sorbonne Université Paris France.

A new genus with a new species from Guangxi Province of China are described in the tribe Sarimini of the family Issidae. Molecular sequences of 18S, 28S and COXI genes are provided for the new taxon. Phylogenetic analysis places this taxon sister to a previously sequenced but not yet described Sarimini genus ' sp. 4'. Taxonomic notes are provided for the genus Yang, 1994. The species Eusarima (Nepalius) iranica Gnezdilov & Mozaffarian, 2011 is transferred to the genus Melichar 1903.
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http://dx.doi.org/10.3897/zookeys.912.39589DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7040043PMC
February 2020

Study of correlations between metabolic risk factors, PWV and hypertension in college students.

Clin Exp Hypertens 2020 May 4;42(4):376-380. Epub 2020 Feb 4.

Department of Hypertension, Henan University People's Hospital, Zhengzhou, Henan, China.

Obesity, dyslipidemia and diabetes have been recognized as risk factors of hypertension in the general population. This study focused on college students and correlations between serum lipids, serum uric acid, fasting plasma glucose, serum creatinine, body mass index, brachial-ankle pulse wave velocity and hypertension. The results suggested that there was no relevance between serum lipids and hypertension. However, it came out that hypertension is associated with normal fasting plasma glucose, BMI (thinness and normal weight), normal serum creatinine. A total of 5185 students were enrolled, and their blood pressure, height and weight were measured. 285 of the students were selected to take physical examinations including serum uric acid, total cholesterol, low-density lipoprotein cholesterol, triglyceride, high-density lipoprotein cholesterol, fasting plasma glucose, serum creatinine, blood urea nitrogen, ankle-brachial index and brachial-ankle pulse wave velocity measured. The values of systolic blood pressure(SBP), diastolic blood pressure(DBP), serum uric acid, fasting plasma glucose(FPG), serum creatinine, body mass index(BMI), and brachial-ankle pulse wave velocity(baPWV) were higher in hypertension group than those in non-hypertension group ). Logistic regression showed correlations between hypertension and hyperuricemia (), obesity (), fasting plasma glucose () and brachial-ankle pulse wave velocity (). The was . We can draw a conclusion that hyperuricemia, obesity, fasting plasma glucose and brachial-ankle pulse wave velocity, listed in descending order of influence, are risk factors for hypertension in college students.
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http://dx.doi.org/10.1080/10641963.2020.1723617DOI Listing
May 2020

Nano-puerarin regulates tumor microenvironment and facilitates chemo- and immunotherapy in murine triple negative breast cancer model.

Biomaterials 2020 03 17;235:119769. Epub 2020 Jan 17.

Division of Pharmacoengineering and Molecular Pharmaceutics, Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599, United States. Electronic address:

Tumor associated fibroblasts (TAFs) are key stromal cells mediating the desmoplastic reaction and being partially responsible for the drug-resistance and immunosuppressive microenvironment formation in solid tumors. Delivery of genotoxic drugs off-targetedly to kill TAFs results in production of Wnt16 which renders the neighboring tumor cells drug resistant as shown in our previous study (PMC4623876). Our current approach looks for means to deactivate, rather than kill, TAFs. Reactive oxygen species (ROS) are the central hub of multiple profibrogenic pathways and indispensable for TAFs activation. Herein, puerarin was identified to effectively downregulate ROS production in the activated myofibroblast. In this study, a novel puerarin nanoemulsion (nanoPue) was developed to improve the solubility and bioavailability of puerarin. NanoPue significantly deactivated the stromal microenvironment (e.g., ~6-fold reduction of TAFs in nanoPue treated mice compared with the PBS control, p < 0.0001) and facilitated chemotherapy effect of nano-paclitaxel in the desmoplastic triple-negative breast cancer (TNBC) model. Moreover, the removal of the physical barrier increased intra-tumoral infiltration of cytotoxic T cell by 2-fold. This activated immune microenvironment allowed nanoPue to synergize PD-L1 blockade therapy in TNBC model.
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http://dx.doi.org/10.1016/j.biomaterials.2020.119769DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7093100PMC
March 2020

Fixation augmentation using titanium cage packing with xenograft in the treatment of tibial plateau fractures.

Injury 2020 Feb 14;51(2):490-496. Epub 2019 Oct 14.

Department of Orthopaedics, Chinese PLA General Hospital, No. 28 Fuxing Road, Beijing 100853, China. Electronic address:

Objectives: To evaluate a new surgical technique concerning titanium cage packing with xenograft demineralized bone matrix bovine augmentation in the management of subchondral bone defects associated with tibial plateau fractures.

Methods: All patients underwent plate fixation augmentation using titanium cage packing with xenograft, with the help of digital medical software of Mimics and 3-matic preoperatively. Duration of the surgical procedure, intraoperative bleeding volume, bone union time, quality of reduction and alignment, fracture healing, complications, and functional outcomes based on Oxford knee outcome score were recorded.

Results: A total of 18 patients were followed for average 18.1 months (range, 12-24 months). The average radiographic bony union time was 12.8 weeks (range, 11-17 weeks) and full weight bearing time was 13.4 weeks (range, 11-16 weeks) respectively. At one-year follow-up, the mean degrees of knee extension were 2.7° (range, 0-6) and the mean degrees of knee flexion were 122.0° (range, 112-134). The articular step-off was unchanged from 0.9 ± 0.6 mm (range, 0-1.8 mm) to 1.0 ± 0.6 mm (0-1.9 mm) (P = 0.512). All the cases had satisfactory reduction. No secondary loss of reduction was found over the time period studied. Superficial infection was found in one case, and resolved with closed treatment. No implant failures were noted. The average Oxford knee score was 19.9 ± 5.4 (range, 12-30) at the final follow-up.

Conclusion: Fixation augmentation using titanium cage packing with xenograft augmentation is a safe and effective way to treat the depressed tibial plateau fractures.
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http://dx.doi.org/10.1016/j.injury.2019.10.025DOI Listing
February 2020

The impact of a new genus on the molecular phylogeny of Hemisphaeriini (Hemiptera, Fulgoromorpha, Issidae).

Zookeys 2019 14;880:61-74. Epub 2019 Oct 14.

Key Laboratory of Southwest China Wildlife Resources Conservation of the Ministry of Education, China West Normal University, Nanchong, Sichuan Province, 637009, China China West Normal University Nanchong China.

A new genus, of the family Issidae (Hemisphaeriinae, Hemisphaeriini) is described from Guangxi Province of China. A revised molecular analysis for the Hemisphaeriini based on partial sequences of 18S, 28S, COXI and Cytb, provides evidence for a new lineage within the subtribe Mongolianina. With two subgroups of genera now identified, the monophyly of Mongolianina is discussed from both a morphological and a molecular basis.
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http://dx.doi.org/10.3897/zookeys.880.36828DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6803356PMC
October 2019

Prevalence of CYP17A1 gene mutations in 17α-hydroxylase deficiency in the Chinese Han population.

Clin Hypertens 2019 15;25:23. Epub 2019 Oct 15.

1Department of Hypertension, Henan Provincial People's Hospital; People's Hospital of Zhengzhou University, Zhengzhou, 450003 Henan China.

Background: 17α-hydroxylase deficiency is a rare autosomal recessive disorder caused by mutations in the cytochrome P450 family 17 subfamily A member 1 gene. The major clinical presentation includes hypertension, hypokalemia, male pseudohermaphroditism and female gonadal dysplasia. Hundreds of pathogenic variants have been reported in this disorder, and some common mutations were found to be race-specific.

Case Presentation: In this study, we reported 5 Chinese girls with 17α-hydroxylase deficiency from Henan Province. The patients all came to the hospital for hypertension, and they also presented with sexual infantilism. The average age of the patients was 14 years old, ranging from 12 to 17 years old. They all had reduced blood cortisol, estradiol (E2), and testosterone (TESTO) and increased adrenocorticotropic hormone (ACTH), follicle-stimulating hormone (FSH), and luteinizing hormone (LH). They all had the appearance of females; however, three of the chromosome karyotypes were 46XX, and two were 46XY.

Conclusions: All of the patients carried a mutation on the 329 amino acid of CYP17A1 exon 6. By summarizing the currently known pathogenic mutations of 17α-hydroxylase deficiency, we demonstrated the prevalence of these gene mutations in Chinese Han and non-Chinese populations.
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http://dx.doi.org/10.1186/s40885-019-0128-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6792268PMC
October 2019

Synergetic Effect of EP1 Receptor Antagonist and (-)-Epigallocatechin-3-gallate in Hepatocellular Carcinoma.

Pharmacology 2019 21;104(5-6):267-275. Epub 2019 Aug 21.

Department of Pharmacology, School of Basic Medical Sciences, Anhui Medical University, Hefei, China,

Epigallocatechin-3-gallate (EGCG), the principal catechin of green tea, modulates different molecular mechanisms underlying hepatocellular carcinoma (HCC). Accumulating studies showed that the activation of prostaglandin (PG) receptor EP1 promotes cell migration and invasion in different cancers, which could be inverted by blocking the EP1 receptor. This study investigated the synergetic effects of EP1-selective antagonist ONO-8711 and EGCG treatment on HCC to better understand the potential strategy to treat HCC. We found that EGCG significantly inhibited PGE2 and EP1-selective agonist induced migration of HCC cells and increased the ratio of Bax/Bcl-2 even in the presence of ONO-DI-004 or PGE2. ONO-8711 significantly inhibited PGE2-induced HCC proliferation while increased the inhibitory effect of EGCG on HCC cell viability and migration ability compared with EGCG alone. These findings suggest that a combination of ONO-8711 and EGCG is a potential treatment for HCC therapy.
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http://dx.doi.org/10.1159/000502076DOI Listing
May 2020
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