Publications by authors named "Mengfan Wu"

25 Publications

  • Page 1 of 1

Ultrasensitive and Simultaneous Detection of Multielements in Aqueous Samples Based on Biomimetic Array Combined with Laser-Induced Breakdown Spectroscopy.

Anal Chem 2021 Jul 16. Epub 2021 Jul 16.

Research Center of Analytical Instrumentation, School of Mechanical Engineering, Sichuan University, Chengdu 610065, China.

Ultrasensitive detection of metallic elements in liquids has attracted considerable attention in fields such as environmental pollution monitoring and drinking water quality control. Hence, it is of great significance to develop a sensitive and simultaneous detection strategy for multiple metal elements in liquid. Laser-induced breakdown spectroscopy (LIBS) technology shows unique advantages because of its simple, rapid, and real-time in situ detection, but the laser energy will be greatly attenuated in the liquids; thus, the sensitivity of LIBS for direct detection of metal elements in liquid samples will decrease sharply. In this study, inspired by the structure of beetle's back, a superhydrophobic biomimetic interface with hydrophilic array was prepared for enriching low-concentration targets into detection regions, and the biomimetic array LIBS (BA-LIBS) was successfully established. The ultrasensitive and simultaneous detection of nine metal elements in drinking water was realized based on the effective enrichment method. The limits of detection of the nine metal elements in mixed solution ranged from 8.3 ppt to 13.49 ppb. With these excellent properties, this facile and ultrasensitive BA-LIBS strategy might provide a new idea for the prevention and control of metal hazards in the liquid environment.
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http://dx.doi.org/10.1021/acs.analchem.1c01484DOI Listing
July 2021

Comparison of Conventional and Platelet-Rich Plasma-Assisted Fat Grafting: A Systematic Review and Meta-analysis.

J Plast Reconstr Aesthet Surg 2021 Jun 12. Epub 2021 Jun 12.

Division of Plastic Surgery, Department of Surgery, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, 02115, USA. Electronic address:

Background: Autologous fat grafting (FG) is a popular technique for soft-tissue augmentation, but the fat survival rate is unpredictable. Platelet-rich plasma (PRP) has emerged as an adjuvant to enhance fat graft survival.

Objectives: This literature review and meta-analysis aimed to investigate the effect of PRP on the survival rate of fat grafting.

Methods: A comprehensive systematic literature search was done to identify clinical studies on PRP and fat cotransplantation in PubMed, Cochrane Library, Web of Science, and EMBASE databases up to May 2020. The reference lists of selected articles were reviewed to identify any additional related articles. A meta-analysis was conducted to compare PRP + FG and conventional FG in terms of fat graft survival rate, patient satisfaction rate, and recovery time after surgery.

Results: Eleven studies consisting of 1125 patients were analyzed. Patients were followed up from 3 to 24 months post-FG. The fat survival rate varied from 20.5% to 54.8% in FG alone and from 24.1% to 89.2% in the PRP + FG groups. The survival rate was significantly higher and recovery time was significantly lower in the PRP + FG group than in the FG alone group. However, there was no significant difference in the patient satisfaction rate between the groups.

Conclusions: This study demonstrates that PRP-enhanced fat transplantation has better efficacy than conventional fat grafting. Further studies are required to provide the optimum concentration of PRP and the long-term efficacy of the technique. There is not enough evidence to compare the rate of complications with PRP and fat cotransplantation and conventional fat grafting.
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http://dx.doi.org/10.1016/j.bjps.2021.05.046DOI Listing
June 2021

The Effect of Obesity on Inpatient Outcomes in Lower Extremity Trauma: A Systematic Review and Meta-analysis.

J Trauma Acute Care Surg 2021 Jul 2. Epub 2021 Jul 2.

Division of Plastic Surgery, Department of Surgery, Brigham and Women's Hospital and Harvard Medical School, 75 Francis St., Boston, USA Department of Traumatic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 4300000, P. R. China Department of Plastic Surgery, Peking University Shenzhen Hospital, Shenzhen, Guangdong, 518036, P. R. China.

Objectives: Obesity is a growing global health problem and a well-recognized risk factor for many medical conditions. This meta analysis was conducted to assess the effect of obesity on overall complication occurrence, mortality and hospital length of stay in patients with nonpathological lower limb trauma.

Data Sources: The EMBASE, PUBMED and MEDLINE electronic databases were searched from inception to 1 April 2020 for studies published in English. References cited by chosen studies were also checked manually for inclusion.

Study Selection: Studies chosen for the analysis were prospective observational or retrospective cohort studies reporting on total complications of patients with acute traumatic, non-pathological, lower limb fractures that required internal fixation, with or without other underlying conditions.

Data Extraction: Two investigators independently reviewed the full text of eligible studies for inclusion and extracted data. Inconsistency was resolved through consultation with other authors.

Data Synthesis: 16 studies with 404,414 patients were investigated in this study. The data showed obesity was related with increased total complications (OR = 0.65, 95%CI 0.51, 0.83, p < 0.01), increased wound complications (OR = 0.41, 95%CI 0.25, 0.66, p < 0.01), and increased mortality rate (OR = 0.64, 95%CI 0.45, 0.91, p < 0.05). Six cohort studies also showed prolonged hospital length of stay in obese patients.

Conclusions: Our results suggest that obesity is associated with increased complication and mortality rates, as well as longer hospital length of stay in patients with lower limb trauma who required surgical treatment. These findings may raise attention to optimize surgical technique and develop individualized treatment for obese patients.

Study Type: Systematic Reviews.

Level Of Evidence: Level III.
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http://dx.doi.org/10.1097/TA.0000000000003328DOI Listing
July 2021

Influence of Three Auditory Profiles on Aided Speech Perception in Different Noise Scenarios.

Trends Hear 2021 Jan-Dec;25:23312165211023709

Institute of Clinical Research, Faculty of Health Sciences, University of Southern Denmark, Odense, Denmark.

Hearing aid (HA) users differ greatly in their speech-in-noise (SIN) outcomes. This could be because the degree to which current HA fittings can address individual listening needs differs across users and listening situations. In two earlier studies, an auditory test battery and a data-driven method were developed for classifying HA candidates into four distinct auditory profiles differing in audiometric hearing loss and suprathreshold hearing abilities. This study explored aided SIN outcome for three of these profiles in different noise scenarios. Thirty-one older habitual HA users and six young normal-hearing listeners participated. Two SIN tasks were administered: a speech recognition task and a "just follow conversation" task requiring the participants to self-adjust the target-speech level. Three noise conditions were tested: stationary speech-shaped noise, speech-shaped babble noise, and speech-shaped babble noise with competing dialogues. Each HA user was fitted with three HAs from different manufacturers using their recommended procedures. Real-ear measurements were performed to document the final gain settings. The results showed that HA users with mild hearing deficits performed better than HA users with pronounced hearing deficits on the speech recognition task but not the just follow conversation task. Moreover, participants with pronounced hearing deficits obtained different SIN outcomes with the tested HAs, which appeared to be related to differences in HA gain. Overall, these findings imply that current proprietary fitting strategies are limited in their ability to ensure good SIN outcomes, especially for users with pronounced hearing deficits, for whom the choice of device seems most consequential.
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http://dx.doi.org/10.1177/23312165211023709DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8246576PMC
July 2021

Low-cost smartphone-based LIBS combined with deep learning image processing for accurate lithology recognition.

Chem Commun (Camb) 2021 Jul;57(58):7156-7159

Research Centre of Analytical Instrumentation, School of Mechanical Engineering, Sichuan University, Chengdu, China 610065, P. R. China.

A low-cost and multi-channel smartphone-based spectrometer was developed for LIBS. As the CMOS detector is two-dimensional, simultaneous multichannel detection was achieved by coupling a linear array of fibres for light collection. Thus, besides the atomic information, the spectral images containing the propagation and spatial distribution characters of a laser induced plasma plume could be recorded. With these additional features, accurate rock type prediction was achieved by processing the raw data directly through a deep learning model.
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http://dx.doi.org/10.1039/d1cc01844bDOI Listing
July 2021

Adipose matrix complex: a high-rigidity collagen-rich adipose-derived material for fat grafting.

Aging (Albany NY) 2021 06 9;13(11):14910-14923. Epub 2021 Jun 9.

Department of Plastic and Cosmetic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, P.R. China.

Due to the low percentage of collagen, the rigid support capacity of fat grafts remains unsatisfactory for some clinical applications. In this study, we evaluated a strategy in which adipose matrix complex (AMC) was collected via a mechanical process and transplanted for supportive filling of the face. Our AMC samples were collected from adipose tissue by a filter device consisting of a sleeve, three internal sieves, and a filter bag (100 mesh). AMC derived from adipose tissue had fewer cells than Coleman fat, but much higher levels of collagen and stiffness. Retention rates 90 days after transplantation in nude mice were higher for AMC than for Coleman fat (75±7.5% 42±13.5%; P < 0.05). In addition, AMC maintained a higher stiffness (~6 kPa ~2 kPa; P < 0.01) and stably retained a higher level of collagen. Our findings demonstrate that mechanical collection of AMC from adipose tissue is a practical method for improving fat graft retention and rigid support. This strategy has the potential to improve the quality of lipoaspirates for patients requiring rigid supportive filling.
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http://dx.doi.org/10.18632/aging.203120DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8221321PMC
June 2021

Adherence to Personal Protective Equipment Guidelines During the COVID-19 Pandemic Among Health-Care Personnel: A Louisiana Case Study.

Disaster Med Public Health Prep 2021 Jun 8:1-4. Epub 2021 Jun 8.

Division of Plastic Surgery, Department of Surgery, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts, USA.

Objective: The aim of this study was to determine the extent that appropriate personal protective equipment (PPE), per Centers for Disease Control and Prevention (CDC) guidance, was used during the coronavirus diseases 2019 (COVID-19) pandemic by health-care personnel (HCP) in Louisiana in 5 clinical settings.

Methods: An online questionnaire was distributed to the LA Nursery registry. Appropriate use of PPE in each of the 5 clinical scenarios was defined by the authors based on CDC guidelines. The scenarios ranged from communal hospital space to carrying out aerosol generating procedures (AGPs). A total of 1760 HCP participated between June and July 2020.

Results: The average adherence in LA was lowest for the scenario of carrying out AGPs at 39.5% compliance and highest for the scenario of patient contact when COVID-19 not suspected at 82.8% compliance. Adherence among parishes varied widely. Commentary to suggest a shortage of PPE supply and the practice of re-using PPE was strong.

Conclusions: Use of appropriate PPE varied by setting. It was higher in scenarios where only face masks (or respirators) were the standard (ie, community hospital or when COVID-19 not suspected) and lower in scenarios where additional PPE (eg, gloves, eye protection, and isolation gown) was required.
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http://dx.doi.org/10.1017/dmp.2021.176DOI Listing
June 2021

Performance evaluation of medical service for breast cancer patients based on diagnosis related groups.

BMC Health Serv Res 2021 May 24;21(1):496. Epub 2021 May 24.

Department of Medical Records Management, The First Affiliated Hospital of Zhengzhou University, 1# Jian She Dong Road, Er Qi Distict, Zhenzhou, China.

Background: To evaluate the performance of medical service for patients with breast cancer in Henan Province, China, using diagnosis related groups (DRGs) indicators and to provide data to inform practices and policies for the prevention and control of breast cancer.

Methods: The data were collected from the front pages of medical records (FPMR) of all hospitals above class II that admitted breast cancer patients in Henan Province between 2016 and 2019. Breast cancer patients were the subjects in our study. China DRGs (CN-DRGs) was used as a risk adjustment tool. Three indicators, including the case mix index (CMI), number of DRGs, and total weight, were used to evaluate the range of available services for patients with breast cancer, while indicators including the charge efficiency index (CEI), time efficiency index (TEI) and inpatient mortality of low-risk group cases (IMLRG) were used to evaluate medical service efficiency and medical safety.

Results: Between 2016 and 2019, there were 103,760 patients with breast cancer. The total weight increased over the study period at an average annual rate of 21.71%. The TEI decreased over the study period by 15.60%. The CEI exhibited an increasing trend, but the average annual rate of increase was small (2.94%). The IMLRP was 0.02, 0, 0 and 0.01% in 2016, 2017, 2018 and 2019, respectively.

Conclusion: The performance of medical service improved between 2016 and 2019 for breast cancer patients discharged from study hospitals in Henan Province. The main area of improvement was in the range of available services, but medical institutions must still make efforts to improve the efficiency of medical services and ensure medical safety. DRGs is an effective evaluation tool.
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http://dx.doi.org/10.1186/s12913-021-06439-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147397PMC
May 2021

Novel Use of Pennig External Fixation for the Treatment of Pediatric Syndactyly.

Tech Hand Up Extrem Surg 2021 Mar 1. Epub 2021 Mar 1.

Departments of Orthopedics Geriatrics, Union hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan Hubei Department of Plastic and Cosmetic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China Department of Surgery, Division of Plastic Surgery, Brigham and Women's Hospital and Harvard Medical School, Boston, MA.

Syndactyly is one of the most common congenital limb malformations, second only to polydactyly. Syndactyly is treated with surgical intervention. During separation, free skin grafting or skin flap transplantation is required to allow for adequate skin closure. We introduce a novel application method of the Pennig external fixation device for the management of pediatric syndactyly without skin grafting. The Pennig minifixator was used in syndactyly to expand the web space soft tissue in order to allow a Z-plasty to be performed in a second operation. All patients had developed sufficient skin tissue from the external fixation. No infection, cutaneous necrosis or nonunion was noted in any of the patients. All patients had a satisfactory outcome in terms of final appearance and digit function. On the basis of the principle of strain-stress, use of the Penning external minifixator allows development of sufficient tissue in the web space for a second phase of reconstruction in syndactyly. The need for skin grafting and risk of blood vessel damage were avoided in this study, and, the incidence of skin necrosis and scar formation was reduced. Both the patients' families and our research members expressed high satisfaction with the postoperative function and appearance. Overall, application of the Pennig external fixation frame in our novel method is effective for the management of syndactyly and should be studied further. Level of Evidence: Level IV, case series.
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http://dx.doi.org/10.1097/BTH.0000000000000342DOI Listing
March 2021

Adherence to Personal Protective Equipment Guidelines During the COVID-19 Pandemic Among Health Care Personnel in the United States.

Disaster Med Public Health Prep 2021 Jan 8:1-3. Epub 2021 Jan 8.

Division of Plastic Surgery, Department of Surgery, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA.

Objectives: Protecting frontline health care workers with personal protective equipment (PPE) is critical during the coronavirus disease (COVID-19) pandemic. Through an online survey, we demonstrated variable adherence to the Centers for Disease Control and Prevention (CDC) PPE guidelines among health care personnel (HCP).

Methods: CDC guidelines for optimal and acceptable PPE usage in common situations faced by frontline health care workers were referenced to create a short online survey. The survey was distributed to national, statewide, and local professional organizations across the United States and to HCP, using a snowball sampling technique. Responses were collected between June 15 and July 17, 2020.

Results: Responses totaling 2245 were received from doctors, nurses, midwives, paramedics, and medical technicians in 44 states. Eight states with n > 20 (Arizona, California, Colorado, Louisiana, Oregon, South Carolina, Texas, and Washington) and a total of 436 responses are included in the quantitative analysis. Adherence to CDC guidelines was observed to be highest in the scenario of patient contact when COVID-19 was not suspected (86.47%) and lowest when carrying out aerosol generating procedures (AGPs) (42.47%).

Conclusions: Further research is urgently needed to identify the reasons underlying variability between professions and regions to pinpoint strategies for maximizing adherence and improving the safety of HCPs.
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http://dx.doi.org/10.1017/dmp.2021.12DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8010287PMC
January 2021

The Rising Interest in Canthoplasty: An Analysis of Online Search Trends.

J Craniofac Surg 2020 Nov 23. Epub 2020 Nov 23.

Angiogenesis Laboratory, Ophthalmology Department, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, MA.

Canthoplasty as a cosmetic procedure appears to be on the rise in the West. Online search query data offers a powerful tool for analyzing population trends, including changes in patient interest in surgical procedures. Cosmetic surgeons can utilize the internet to increase patient education and interest, as well as to provide information and address misinformation. In this study we sought to verify the increase in cosmetic canthoplasty, for the first time, through analysis of Internet search data, and to establish trends in the interest of Internet users for cosmetic canthoplasty. These trends were subsequently compared with trends in literature publication to establish whether there is a correlation between patient and surgeon interest in the procedure.
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http://dx.doi.org/10.1097/SCS.0000000000007272DOI Listing
November 2020

Toehold-mediated strand displacement reaction formation of three-way junction DNA structure combined with nicking enzyme signal amplification for highly sensitive colorimetric detection of Salmonella Typhimurium.

Anal Chim Acta 2020 Dec 18;1139:138-145. Epub 2020 Sep 18.

Research Center of Analytical Instrumentation, Key Laboratory of Bio-resource and Eco-environment, Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610065, Sichuan, China. Electronic address:

The detection of Salmonella Typhimurium (S.typhimurium) is of great importance in food safety field. Colorimetric strategy is particularly appealing for S. typhimurium identification because of its user-friendliness and instrument-free. However, the existing colorimetric strategies still meet the challenges of low sensitivity, tedious nucleic acid extraction and expensive labeling processes. Herein, a high sensitivity and label-free colorimetric sensing strategy for S. typhimurium detection without nucleic acid extraction is constructed. Specifically, the proposed strategy is based on three-way junction (3WJ) DNA branched structure combined with nicking enzyme signal amplification (NESA). In the presence of target, cascaded signal amplification is initiated through a series of toehold-mediated strand displacement reactions (TSDRs) to recycle the trigger DNA causing formation of the numerous 3WJ DNA branched structures (3WJ-TSDRs). Then, the branches of 3WJ-TSDRs are fully utilized to hybridize with the DNAzyme signal probes to initiate NESA in the presence of Nt. BbvCI, which making every branch has a function of signal amplification. Finally, DNAzyme signal probes (green) were completely split into two fragments (colorless). The application of NESA in the branches of 3WJ-TSDRs offers a highly sensitive detection of S. typhimurium with a low limit of detection of 42 CFU mL. Besides, the colorimetric sensing strategy also shows strong anti-interference. The capability of the colorimetric sensing strategy in spiked samples was also investigated, showing a more intuitive results and fast detection in compare with the traditional plate counting method. With these characteristics, the proposed sensing strategy based on 3WJ-TSDRs and NESA is a promising tool for new point-of-care (POC) applications in food safety.
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http://dx.doi.org/10.1016/j.aca.2020.09.023DOI Listing
December 2020

Novel application of autologous micrografts in a collagen-glycosaminoglycan scaffold for diabetic wound healing.

Biomed Mater 2020 Oct 22. Epub 2020 Oct 22.

Brigham and Women's Hospital, Boston, UNITED STATES.

Background: Therapeutic strategies that successfully combine two techniques-autologous micrografting and biodegradable scaffolds-offer great potential for improved wound repair and decreased scarring. In this study we evaluate the efficacy of a novel modification of a collagen-glycosaminoglycan scaffold with autologous micrografts using a murine dorsal wound model.

Methods: db/db mice underwent dorsal wound excision and were treated with a collagen-glycosaminoglycan scaffold (CGS), a modified collagen-glycosaminoglycan scaffold (CGS+MG) or simple occlusive dressing (Blank). The modified scaffold was created by harvesting full thickness micrografts and transplanting these into the collagen-glycosaminoglycan membrane. Parameters of wound healing, including cellular proliferation, collagen deposition, keratinocyte migration, and angiogenesis were assessed.

Results: The group treated with the micrograft-modified scaffold healed at a faster rate, showed greater cellular proliferation, collagen deposition, and keratinocyte migration with higher density and greater maturity of microvessels. The grafts remained viable within the scaffold with no evidence of rejection. Keratinocytes were shown to migrate from the wound border and from the micrograft edges towards the center of the wound, while cellular proliferation was present both at the wound border and wound bed.

Conclusion: We report successful treatment of diabetic wounds with a novel collagen-glycosaminoglycan scaffold modified with full-thickness automicrografts. Differences in cellular migration and proliferation offer maiden evidence on the mechanisms of wound healing. Clinically, the successful scaffold engraftment, micrograft viability and improved wound healing offer promising results for the development of a new therapeutic modality for wound repair.
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http://dx.doi.org/10.1088/1748-605X/abc3dcDOI Listing
October 2020

Investigating the Effects of Four Auditory Profiles on Speech Recognition, Overall Quality, and Noise Annoyance With Simulated Hearing-Aid Processing Strategies.

Trends Hear 2020 Jan-Dec;24:2331216520960861

Institute of Clinical Research, Faculty of Health Sciences, University of Southern Denmark, Odense, Denmark.

Effective hearing aid (HA) rehabilitation requires personalization of the HA fitting parameters, but in current clinical practice only the gain prescription is typically individualized. To optimize the fitting process, advanced HA settings such as noise reduction and microphone directionality can also be tailored to individual hearing deficits. In two earlier studies, an auditory test battery and a data-driven approach that allow classifying hearing-impaired listeners into four auditory profiles were developed. Because these profiles were found to be characterized by markedly different hearing abilities, it was hypothesized that more tailored HA fittings would lead to better outcomes for such listeners. Here, we explored potential interactions between the four auditory profiles and HA outcome as assessed with three different measures (speech recognition, overall quality, and noise annoyance) and six HA processing strategies with various noise reduction, directionality, and compression settings. Using virtual acoustics, a realistic speech-in-noise environment was simulated. The stimuli were generated using a HA simulator and presented to 49 habitual HA users who had previously been profiled. The four auditory profiles differed clearly in terms of their mean aided speech reception thresholds, thereby implying different needs in terms of signal-to-noise ratio improvement. However, no clear interactions with the tested HA processing strategies were found. Overall, these findings suggest that the auditory profiles can capture some of the individual differences in HA processing needs and that further research is required to identify suitable HA solutions for them.
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http://dx.doi.org/10.1177/2331216520960861DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7594216PMC
November 2020

The Recent Development of Hybridization Chain Reaction Strategies in Biosensors.

ACS Sens 2020 10 1;5(10):2977-3000. Epub 2020 Oct 1.

West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, China.

With the continuous development of biosensors, researchers have focused increasing attention on various signal amplification strategies to pursue superior performance for more applications. In comparison with other signal amplification strategies, hybridization chain reaction (HCR) as a powerful signal amplification technique shows its certain charm owing to nonenzymatic and isothermal features. Recently, on the basis of conventional HCR, this technique has been developed and improved rapidly, and a variety of HCR-based biosensors with excellent performance have been reported. Herein, we present a systematic and critical review on the research progress of HCR in biosensors in the last five years, including the newly developed HCR strategies such as multibranched HCR, migration HCR, localized HCR, in situ HCR, netlike HCR, and so on, as well as the combination strategies of HCR with isothermal signal amplification techniques, nanomaterials, and functional DNA molecules. By illustrating some representative works, we also summarize the advantage and challenge of HCR in biosensors, and offer a deep discussion of the latest progress and future development trends of HCR in biosensors.
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http://dx.doi.org/10.1021/acssensors.0c01453DOI Listing
October 2020

Storm-induced sediment resuspension in the Changjiang River Estuary leads to alleviation of phosphorus limitation.

Mar Pollut Bull 2020 Nov 12;160:111628. Epub 2020 Sep 12.

Frontiers Science Center for Deep Ocean Multispheres and Earth System, and Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266100, China; College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266100, China.

This paper presents an incubation experiment with sediment cores from the Changjiang Estuary Mud Area (CEMA) to quantify the release of nutrients due to simulated resuspension. The results show that except for nitrate (NO-N), phosphate (PO-P), ammonium (NH-N), nitrite (NO-N) and silicate (SiO-Si) were released from the sediment to the overlying water, primarily due to desorption (P), dissolution (SiO-Si) and mineralization (NH-N) with only minor direct contributions from the sediment pore water. The significant release of nutrients by resuspension and subsequent processes can alleviate the phosphorus and silicon limitation in water bodies, enhance the growth of phytoplankton, and thus promote the oxygen consumption and ultimately lead to hypoxia. The results of this study are highly relevant for many coastal areas in other parts of the world with large amounts of stored organic matter and nutrients in sediments and frequent perturbation by storm events.
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http://dx.doi.org/10.1016/j.marpolbul.2020.111628DOI Listing
November 2020

Adherence to Personal Protective Equipment Guidelines During the COVID-19 Pandemic: A Worldwide Survey Study.

Br J Surg 2020 Oct 24;107(11):e526-e528. Epub 2020 Aug 24.

Division of Plastic Surgery, Department of Surgery, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, 02115, USA.

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http://dx.doi.org/10.1002/bjs.12001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7460957PMC
October 2020

Plastic Surgery During the COVID-19 Pandemic: The Space, Equipment, Expertise Approach.

Aesthet Surg J 2020 08;40(9):NP574-NP577

Department of Surgery, Division of Plastic Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA.

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http://dx.doi.org/10.1093/asj/sjaa136DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7314169PMC
August 2020

Poly-adenine regulated DNA density on AuNPs to construct efficient DNA walker for microRNA-21 detection.

Talanta 2020 Sep 20;217:121056. Epub 2020 Apr 20.

Research Center of Analytical Instrumentation, Key Laboratory of Bio-resource and Eco-environment, Ministry of Education, College of Life Science, Sichuan University, Chengdu, 610065, Sichuan, PR China. Electronic address:

DNA-modified gold nanoparticles (AuNPs) are useful nanomaterials for detecting multiple molecules. However, their performance is greatly dependent on the density of probe DNA on the surface of AuNPs. Here, we used Poly-adenine (PolyA) to regulate the surface density of probe DNA to achieve a highly efficient DNA walking biosensor system to detection miRNA-21. The movement track of the biosensor system consists of PolyA-DNA probe was connected to AuNPs, and exonuclease III (Exo III) acted as a motor driving the walker movement to achieve signal amplification. By optimizing the length of PolyA, the surface density of probe DNA was changed, thereby affecting the target binding and enzymatic processing of the bound probes, which ultimately enhanced the sensitivity and reduced timeliness of the DNA walker. Furthermore, the designed PolyA-DNA probe exhibits an outstanding sensitivity, due to the effect of density regulation, which is 7.9 times and 11.1 times lower than those of the SH-DNA and the free-DNA, respectively. In addition, the hairpin structure of DNA probe locates fluorophore at a zone adjacent to AuNPs surface, which reduces the background signal by 1.1 times compared with traditional straight probe. In this work, the biosensor system shows a high selectivity towards miRNA-21. Moreover, the biosensor system has been demonstrated to be potentially useful for the miRNA-21 detection in human serum with the recoveries of 93.2%-110.0% and has high repeatability. Considering these advantages, this PolyA-regulated DNA walking biosensor system has great potential as a routine tool for miRNA detection and has wide applications in the field of biomedical analysis.
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http://dx.doi.org/10.1016/j.talanta.2020.121056DOI Listing
September 2020

Self-assembling hydrogel loaded with 5-FU PLGA microspheres as a novel vitreous substitute for proliferative vitreoretinopathy.

J Biomed Mater Res A 2020 12 3;108(12):2435-2446. Epub 2020 Jul 3.

Department of Ophthalmology, Zhujiang Hospital, Southern Medical University, Guangzhou, China.

The vitreous substitute for proliferative vitreoretinopathy (PVR) surgery remains an unmet clinical need in ophthalmology. In our study, we developed an in situ formed hydrogel by crosslinking polyvinyl alcohol (PVA) and chitosan as a potential vitreous substitute. 5-fluorouracil (5-FU) Poly (lactic-co-glycolic acid) (PLGA) microspheres were developed and loaded onto the PVA/chitosan hydrogels to treat PVR. In vitro, PVA/chitosan hydrogels at four concentrations were subjected to morphological, physical, rheological analyses, and cytotoxicity was evaluated together with the characterization of 5-FU PLGA microspheres. In vivo, pharmacologically induce PVR rabbits were performed a vitrectomy. In the PVA group, 3% PVA/chitosan hydrogel was injected into the vitreous cavity. In the PVA/MS group, 3% PVA/chitosan hydrogel and 5-FU PLGA microspheres were injected. In the Control group, phosphate-buffered saline was injected. Therapeutic efficacy was evaluated with postoperative examinations and histological analyses. This study demonstrated that the 3% PVA/chitosan hydrogel showed properties similar to those of the human vitreous and could be a novel in situ crosslinked vitreous substitute for PVR. Loading 5-FU PLGA microspheres onto this hydrogel may represent an effective strategy to improve the prognosis of PVR.
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http://dx.doi.org/10.1002/jbm.a.36995DOI Listing
December 2020

Botulinum Toxin A Improves Supramuscular Fat Graft Retention by Enhancing Angiogenesis and Adipogenesis.

Dermatol Surg 2020 05;46(5):646-652

All authors are affiliated with the Department of Plastic and Cosmetic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, People's Republic of China.

Background: Autologous fat grafting is commonly used for soft-tissue augmentation and reconstruction in plastic surgery. However, long-term graft retention rates are unpredictable, especially in muscle-related fat grafting.

Objective: To determine whether botulinum neurotoxin type A (BoNTA) may improve supramuscular fat grafting retention by reducing muscle movement, thereby enhancing angiogenesis and adipogenesis.

Materials And Methods: Pre-BTX+ nude mice were injected with BoNTA on the right quadriceps femoris and underwent supramuscular fat grafting 1 week later. BTX+ nude mice simultaneously underwent BoNTA injection and transplantation. Control nude mice underwent transplantation without BoNTA. Graft volumes were determined, and grafts underwent histological analyses and immunostaining. CatWalk XT gait analysis was conducted on BTX+ mice.

Results: Pre-BTX+ and BTX+ groups had significantly higher retention rates and exhibited better angiogenesis and adipocyte survival than the Control group.

Conclusion: BoNTA injections improved the long-term retention of supramuscular fat grafts by reducing muscle movement, possibly by augmenting angiogenesis and adipogenesis.
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http://dx.doi.org/10.1097/DSS.0000000000002106DOI Listing
May 2020

TGF-β prevents the denervation-induced reduction of bone formation and promotes the bone regeneration through inhibiting ubiquitin-proteasome pathway.

Biosci Rep 2019 05 14;39(5). Epub 2019 May 14.

Department of Plastic and Cosmetic Surgery, Nanfang Hospital, Southern Medical University, 1838 Guangzhou North Road, Guangzhou, Guangdong 510515, P. R. China

Transforming growth factor beta (TGF-β) can stimulate osteogenesis as a multifunctional protein. The present study was to explore if TGF-β can prevent denervation-induced reduction of bone formation. The 6-week-old male mice were treated with recombinant human TGF-β1 (rhTGF-β1). Bone formation, endochondral bone growth rates, and gene expression of osteoblast markers were measured in the skeletal tissue by real-time PCR. RhTGF-β1 treatment prevented the denervation-induced decrease in bone formation rates, endochondral growth, and expression of Cbfa1/Runx2 (runt-related transcription factor 2), Ostecalcin (OC), and ColIA1. TGF-β1 partially inhibited the denervation-induced ubiquitination of Cbfa1/Runx2 in mouse cancellous bones via ubiquitin-proteasome pathway. TGF-β prevents denervation-induced reduction of bone formation and promotes the bone regeneration through inhibiting ubiquitin-proteasome pathway at least partially.
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http://dx.doi.org/10.1042/BSR20190350DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6522721PMC
May 2019

A multiplex RT-PCR assay for rapid and simultaneous detection of four RNA viruses in swine.

J Virol Methods 2019 07 2;269:38-42. Epub 2019 Apr 2.

State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan 430072, China. Electronic address:

A multiplex reverse transcription polymerase chain rection (mRT-PCR) was developed for simultaneous detection of four RNA viruses in swine. The conserved target sequences directed to classical swine fever virus (CSFV), porcine reproductive and respiratory syndrome virus (PRRSV), porcine epidemic diarrhea virus (PEDV) and transmissible gastroenteritis coronavirus (TGEV) were selected based on alignments of genomic sequences and then specific primers were designed. The mRT-PCR assay was developed and evaluated for its specificity and sensitivity. The expected product from the single viral template was amplified by mRT-PCR and no spurious PCR amplification occurred from the genomic RNA or DNA of other pathogens. For single virus or different combinations of two viruses the detection limit of mRT-PCR was consistent with a single RT-PCR wtith 1 × 10 copies. For different combinations of the three viruses or four viruses, sensitivity of PEDV detection partially decreased. All of positive clinical specimens by the mRT-PCR were identically confirmed using Taqman RT-qPCR. Therefore, the mRT-PCR is a useful tool for epidemiological studies and laboratory diagnosis of single virus and/or mixed infections in swine.
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http://dx.doi.org/10.1016/j.jviromet.2019.04.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7113762PMC
July 2019

Application of External Force Regulates the Migration and Differentiation of Adipose-Derived Stem/Progenitor Cells by Altering Tissue Stiffness.

Tissue Eng Part A 2019 12 20;25(23-24):1614-1622. Epub 2019 Nov 20.

Department of Plastic and Cosmetic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, P.R. China.

Large soft-tissue defects are challenging to reconstruct surgically. Expansion of soft tissue using an external volume expansion (EVE) device is a noninvasive method to improve such reconstruction; however, the underlying mechanism is unclear. In this study, we created fat flaps in Sprague-Dawley rats, applied an external force of 3 or 6 kPa using an EVE device, and investigated the migration and differentiation of adipose-derived stem/progenitor cells (ASCs). In addition, we performed finite element analysis to explore the stiffness of adipose tissue. An external force of 3 kPa promoted the migration and adipogenic differentiation of ASCs. By comparison, an external force of 6 kPa had a larger effect on migration of ASCs, but a smaller effect on adipogenic differentiation of ASCs. External force affected adipose tissue stiffness. In conclusion, external force generated by an EVE device increases the stiffness of adipose tissue, which influences the migration and differentiation of ASCs. The size of the external force can be altered according to the tissue stiffness required at particular time points to promote long-term adipose tissue regeneration. Impact Statement Stem cell therapy in clinic mostly requires the addition of exogenous stem cells, therefore the safety and controllability is always defective. In this study, the external force of external volume expansion regulates adipose-derived stem/progenitor cells (ASCs) migration and differentiation through tissue stiffness. Using tissue engineering without exogenous ASCs can promote long-term adipose tissue regeneration. The findings of this study provide theoretical support for clinical tissue engineering applications and improvements in stem cell therapy.
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http://dx.doi.org/10.1089/ten.TEA.2019.0046DOI Listing
December 2019

Polyhydric polymer-functionalized fluorescent probe with enhanced aqueous solubility and specific ion recognition: A test strips-based fluorimetric strategy for the rapid and visual detection of Fe ions.

Talanta 2017 Aug 12;170:306-313. Epub 2017 Apr 12.

Institute of Medicine and Material Applied Technologies, College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu City, Shandong Province 273165, PR China. Electronic address:

A polyhydric polymer-functionalized probe with enhanced aqueous solubility was designed initially by coupling 1-pyrenecarboxyaldehyde (Pyr) onto poly(vinyl alcohol) (PVA) via the one-step condensation reaction. Polyhydric PVA polymer chains could facilitate the Pyr fluorophore with largely improved aqueous solubility and especially strong cyan fluorescence. Importantly, the fluorescence of the PVA-Pyr probes could thereby be quenched specifically by Fe ions through the strong PVA-Fe interaction triggering the polymeric probe aggregation. Furthermore, a test strips-based fluorimetric method was developed with the stable and uniform probe distribution by taking advantage of the unique film-forming ability and the depression capacity of "coffee-stain" effects of PVA matrix. The as-developed test strips could allow for the rapid and visual detections of Fe ions simply by a dipping way, showing a linear concentration range of 5.00-300μM, with the detection limit of 0.73μM. Moreover, the proposed method was applied to the evaluation of Fe ions in natural water samples, showing the analysis performances better or comparable to those of current detection techniques. This test strips-based fluorimetric strategy promises the extensive applications for the rapid on-site monitoring of Fe ions in environmental water and the outdoor finding of the potential iron mines.
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http://dx.doi.org/10.1016/j.talanta.2017.04.023DOI Listing
August 2017