Publications by authors named "Mengdi Guo"

24 Publications

  • Page 1 of 1

Gas-phase synthesis and deposition of metal-bipyridine complex [M-bpy] (M = Ag, Cu).

Phys Chem Chem Phys 2021 Aug;23(30):16334-16340

Beijing National Laboratory for Molecular Sciences (BNLMS), State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China.

Controllable synthesis of organometallic clusters in the gas phase is a topic of reasonable interest with precisely tunable properties depending on sizes, compositions, and intra-cluster charge-transfer interactions. Here, we have prepared small Agn+ and Cun+ clusters by using a customized magnetron sputtering (MagS) source and observed the gas-phase reactions with 2,2'-bipyridine. It is found that the small silver and copper clusters readily react with bipyridine and form products of [M-bpy1-2]+ (M = Ag, Cu). Quantum chemistry calculations reveal that the bipyridine in both [Ag-bpy1-2]+ and [Cu-bpy1-2]+ takes on cis-conformation with altered N-C-C-N dihedral angles, which is in contrast to the trans-conformation of a free 2,2'-bipyridine molecule itself. In order to unveil the principle of conformational transition, we have fully studied the interactions between the nitrogen atoms of bipyridine and the cationic Ag+ and Cu+, calculated the donor-acceptor orbital overlaps, and analyzed the correlation of their frontier molecular orbital energy levels. Furthermore, by using a soft-landing strategy, we have managed to deposit the [Cu-bpy2]+ complex onto the glass substrates coated with Ag nanoparticles, and recorded the surface-enhanced Raman scattering spectra.
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http://dx.doi.org/10.1039/d1cp02399cDOI Listing
August 2021

Assessing psychological symptom networks related to HIV-positive duration among people living with HIV: a network analysis.

AIDS Care 2021 May 27:1-9. Epub 2021 May 27.

NYU Rory Meyers College of Nursing, New York University, New York City, New York, USA.

This study aims to explore and visualize relationships among multiple psychological symptoms among people living with HIV (PLWH) with different HIV-positive durations and to compare centrality indices and densities of psychological symptom networks. We used subsets of data collected from five designated HIV/AIDS hospitals in China. Networks were constructed among 16 psychological symptoms. Centrality properties, including strength and closeness, were adopted to describe relationships among symptoms. The results showed that PLWH with longer HIV-positive durations had denser emotional networks, which indicated that they had more emotional neuroticism than their newly diagnosed counterparts. Sadness, self-abasement, and self-loathing were the most central psychological symptoms across different HIV-positive durations. Our study suggests the need to provide psychosocial support services targeting PLWH according to changing symptom severity and neuroticism trajectories. Interventions should focus on increasing empathy for PLWH and enhancing the ability to consider the situation from different perspectives to avoid the development of neuroticism in long-term survivors.
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http://dx.doi.org/10.1080/09540121.2021.1929815DOI Listing
May 2021

Psychometric properties of self-reported measures of health-related quality of life in people living with HIV: a systematic review protocol.

JBI Evid Synth 2021 May 14. Epub 2021 May 14.

School of Nursing, Fudan University, Shanghai, China Fudan University Centre for Evidence-based Nursing: A JBI Centre of Excellence, Shanghai, China School of Public Health, Fudan University, Shanghai, China Beijing Administration Institute, Beijing, China School of Public Affairs, Zhejiang University, Hangzhou, China.

Objective: The aim of this review is to identify and assess the psychometric properties of patient-reported outcome measures of health-related quality of life in people living with HIV.

Introduction: Some reviews have been conducted to identify generic and HIV-specific health-related, quality-of-life measures in people living with HIV. However, these studies did not report the psychometric properties of patient-reported outcome measures, making it difficult for researchers to choose one measure from the existing measures to assess health-related quality of life. This review will highlight various psychometric properties of the validation studies rather than reporting the content of measures.

Inclusion Criteria: We will consider studies that aim to validate patient-reported outcome measures of health-related quality of life in people living with HIV and evaluate one or more measurement proprieties. Studies published in English or Chinese and conducted in any country or setting are eligible for inclusion.

Methods: PubMed, Embase (Ovid), CINAHL (EBSCO), PsycINFO (EBSCO), Web of Science, ProQuest Dissertations and Theses, and Cochrane Library (Wiley) will be searched from January 1, 1996 to the present. The evaluation of measurement properties, data extraction, and data synthesis will be conducted according to JBI methodology.

Systematic Review Registration Number: PROSPERO (pending).
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http://dx.doi.org/10.11124/JBIES-20-00190DOI Listing
May 2021

Do Migrants Receive Tuberculosis Education in China? Evidence From the China Migrants Dynamic Survey.

Health Educ Behav 2021 Apr 7:10901981211000309. Epub 2021 Apr 7.

Fudan University School of Nursing, Shanghai, China.

Background: Migrants are the key population for tuberculosis (TB) transmission in China. However, it remains unknown how many migrants have received TB education and through what means.

Objectives: To identify the rate and methods of TB education among migrants in China by using nationally representative data.

Method: This study used secondary data analysis. The data were derived from the China Migrants Dynamic Survey 2014-2017. A total sample of 745,926 migrants was included in the following analysis. Information on TB education was collected through a self-report questionnaire. We used hierarchical logistic regression models to explore the relationship between the independent variables and the receipt of TB education.

Results: Only 30.4% ( = 226,458) received TB education. Among all age-groups, participants between 65 and 69 years old had the highest TB education rate (33.4%). Bulletin boards (86.5%-91%), media (73% to 86.7%), and books/magazines (59.2%-67.4%) were the most common ways for migrants to receive TB education.

Conclusions: Our study showed the rates of TB education in each region of China and indicated the significant disparity among the seven regions. Traditional media, off-line medical consultation, community advocacy, and bulletin boards should be the primary methods of delivering TB education. TB education campaigns targeting migrants with a low socioeconomic status should be actively promoted.
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http://dx.doi.org/10.1177/10901981211000309DOI Listing
April 2021

Early innate and adaptive immune perturbations determine long-term severity of chronic virus and Mycobacterium tuberculosis coinfection.

Immunity 2021 03 29;54(3):526-541.e7. Epub 2021 Jan 29.

Princess Margaret Cancer Center, University Health Network, Toronto, ON M5G 2M9, Canada; Department of Immunology, University of Toronto, Toronto, ON M5S 1A8, Canada. Electronic address:

Chronic viral infections increase severity of Mycobacterium tuberculosis (Mtb) coinfection. Here, we examined how chronic viral infections alter the pulmonary microenvironment to foster coinfection and worsen disease severity. We developed a coordinated system of chronic virus and Mtb infection that induced central clinical manifestations of coinfection, including increased Mtb burden, extra-pulmonary dissemination, and heightened mortality. These disease states were not due to chronic virus-induced immunosuppression or exhaustion; rather, increased amounts of the cytokine TNFα initially arrested pulmonary Mtb growth, impeding dendritic cell mediated antigen transportation to the lymph node and subverting immune-surveillance, allowing bacterial sanctuary. The cryptic Mtb replication delayed CD4 T cell priming, redirecting T helper (Th) 1 toward Th17 differentiation and increasing pulmonary neutrophilia, which diminished long-term survival. Temporally restoring CD4 T cell induction overcame these diverse disease sequelae to enhance Mtb control. Thus, Mtb co-opts TNFα from the chronic inflammatory environment to subvert immune-surveillance, avert early immune function, and foster long-term coinfection.
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http://dx.doi.org/10.1016/j.immuni.2021.01.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7946746PMC
March 2021

Outdoor light at night and risk of coronary heart disease among older adults: a prospective cohort study.

Eur Heart J 2021 02;42(8):822-830

Department of Environmental Health, Boston University School of Public Health, 715 Albany Street, Boston, MA 02118, USA.

Aims: We estimated the association between outdoor light at night at the residence and risk of coronary heart disease (CHD) within a prospective cohort of older adults in Hong Kong.

Methods And Results: Over a median of 11 years of follow-up, we identified 3772 incident CHD hospitalizations and 1695 CHD deaths. Annual levels of outdoor light at night at participants' residential addresses were estimated using time-varying satellite data for a composite of persistent night-time illumination at ∼1 km2 scale. We used Cox proportional hazards models to estimate hazard ratios (HRs) and 95% confidence intervals (CIs) of the association between outdoor light at night at the residence and risk of CHD. The association between light at night and incident CHD hospitalization and mortality exhibited a monotonic exposure-response function. An interquartile range (IQR) (60.0 nW/cm2/sr) increase in outdoor light at night was associated with an HR of 1.11 (95% CI: 1.03, 1.18) for CHD hospitalizations and 1.10 (95% CI: 1.00, 1.22) for CHD deaths after adjusting for both individual and area-level risk factors. The association did not vary across strata of hypothesized risk factors.

Conclusion: Among older adults, outdoor light at night at the residence was associated with a higher risk of CHD hospitalizations and deaths. We caution against causal interpretation of these novel findings. Future studies with more detailed information on exposure, individual adaptive behaviours, and potential mediators are warranted to further examine the relationship between light at night and CHD risk.
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http://dx.doi.org/10.1093/eurheartj/ehaa846DOI Listing
February 2021

A network of immune and microbial modifications underlies viral persistence in the gastrointestinal tract.

J Exp Med 2020 12;217(12)

Princess Margaret Cancer Center, University Health Network, Toronto, Ontario, Canada.

Many pathogens subvert intestinal immunity to persist within the gastrointestinal tract (GIT); yet, the underlying mechanisms that enable sanctuary specifically in this reservoir are unclear. Using mass cytometry and network analysis, we demonstrate that chronic LCMV infection of the GIT leads to dysregulated microbial composition, a cascade of metabolic alterations, increased susceptibility to GI disease, and a system-wide recalibration of immune composition that defines viral persistence. Chronic infection led to outgrowth of activated Tbet-expressing T reg cell populations unique to the GIT and the rapid erosion of pathogen-specific CD8 tissue-resident memory T cells. Mechanistically, T reg cells and coinhibitory receptors maintained long-term viral sanctuary within the GIT, and their targeting reactivated T cells and eliminated this viral reservoir. Thus, our data provide a high-dimensional definition of the mechanisms of immune regulation that chronic viruses implement to exploit the unique microenvironment of the GIT and identify T reg cells as key modulators of viral persistence in the intestinal tract.
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http://dx.doi.org/10.1084/jem.20191473DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7953734PMC
December 2020

Ligand accommodation causes the anti-centrosymmetric structure of AuCu clusters with near-infrared emission.

Nanoscale 2020 Jul 6;12(27):14801-14807. Epub 2020 Jul 6.

Beijing National Laboratory for Molecular Sciences (BNLMS) and State Key Laboratory for Structural Chemistry of Unstable and Stable Species, and Key Laboratory of Photochemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, P.R. China.

We synthesized an [AuCu(PPh)(SPy)] nanocluster co-capped by phosphine and thiolate ligands. Interestingly, this AuCu cluster corresponds to an anti-centrosymmetric structure with the four copper atoms coordinated to the mixed ligands on the same side of the Au icosahedron, which is in sharp contrast to the [AuCu(PPhPy)(SPhtBu)] and [AuCu(PPh)(SPy)] clusters which possess highly symmetric structures with well-separated Cu adatoms. Both [AuCu(PPh)(SPy)] and [AuCu(PPh)(SPy)] clusters correspond to 8 valence electron superatoms with large HOMO-LUMO gaps, respectively. The difference in structure is rooted in the nature of the mixed ligands, with the bidentate SPy binding strongly to Cu on both binding sites (-N-Cu and Au-SR-Cu) leading to the co-linking of adjacent Cu atoms, while the bidentate PPhPy binds Cu on one site and Au on the other giving rise to a separation of the Cu atoms even in the presence of relatively higher monomer concentration. Both [AuCu(PPh)(SPy)] and [AuCu(PPh)(SPy)] display emissions in the near-IR regions. TD-DFT calculations reproduce the spectroscopic results with specified excited states, shedding light on the geometric and electronic behaviors of the ligand-protected AuM clusters.
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http://dx.doi.org/10.1039/d0nr02448aDOI Listing
July 2020

Nodal Global Efficiency in Front-Parietal Lobe Mediated Periventricular White Matter Hyperintensity (PWMH)-Related Cognitive Impairment.

Front Aging Neurosci 2019 10;11:347. Epub 2019 Dec 10.

Department of Neurology, Drum Tower Hospital, Medical School and The State Key Laboratory of Pharmaceutical Biotechnology, Institute of Brain Science, Nanjing University, Nanjing, China.

White matter hyperintensity (WMH) is widely observed in the elderly population and serves as a key indicator of cognitive impairment (CI). However, the underlying mechanism remains to be elucidated. Herein, we investigated the topological patterns of resting state functional networks in WMH subjects and the relationship between the topological measures and CI. A graph theory-based analysis was employed in the resting-state functional magnetic resonance scans of 112 subjects (38 WMH subjects with cognitive impairment without dementia (CIND), 36 WMH subjects with normal cognition and 38 healthy controls (HCs), and we found that WMH-CIND subjects displayed decreased global efficiency at the levels of the whole brain, specific subnetworks [fronto-parietal network (FPN) and cingulo-opercular network (CON)] and certain nodes located in the FPN and CON, as well as decreased local efficiency in subnetworks. Our results demonstrated that nodal global efficiency in frontal and parietal regions mediated the impairment of information processing speed related to periventricular WMH (PWMH). Additionally, we performed support vector machine (SVM) analysis and found that altered functional efficiency can identify WMH-CIND subjects with high accuracy, sensitivity and specificity. These findings suggest impaired functional networks in WMH-CIND individuals and that decreased functional efficiency may be a feature of CI in WMH subjects.
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http://dx.doi.org/10.3389/fnagi.2019.00347DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6914700PMC
December 2019

Provincial and Age Disparity on Chronic Disease Education Among Migrants in China: The Migrants Population Dynamic Monitoring Survey.

Inquiry 2019 Jan-Dec;56:46958019895897

New York University Rory Meyers College of Nursing, New York, USA.

Chronic diseases have become serious threats to public health in China; the risk is particularly high for internal migrants. Chronic disease education is a key to the prevention and control of chronic diseases for such population. The national population-based Migrants Population Dynamic Monitoring Survey (MPSMA) was used to examine the current status and delivery methods of chronic disease education among internal migrants, from both provincial level and individual's level. The study population included 402 587 internal migrants. Multilevel logistic regression was used to investigate factors that were related to chronic diseases education. In total, only 33.9% of the participants received chronic disease education. In the final model, parameter estimates on key variables from both individual and provincial level were significant ( < .001). Participants from provinces with higher level of health care resources and lower density of internal migrants were more likely to receive chronic disease education. The percentage and methods of receiving education varied across different age groups. This study suggests that future chronic disease education in China need to be more focused on areas with high density of internal migrants and younger internal migrants with low level of education and income. Attention should be paid to use tailored education methods to different populations.
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http://dx.doi.org/10.1177/0046958019895897DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6927201PMC
June 2020

GCN2 drives macrophage and MDSC function and immunosuppression in the tumor microenvironment.

Sci Immunol 2019 12;4(42)

Tumor Immunotherapy Program, Princess Margaret Cancer Centre, University Health Network, Toronto, ON, Canada.

General control nonderepressible 2 (GCN2) is an environmental sensor controlling transcription and translation in response to nutrient availability. Although GCN2 is a putative therapeutic target for immuno-oncology, its role in shaping the immune response to tumors is poorly understood. Here, we used mass cytometry, transcriptomics, and transcription factor-binding analysis to determine the functional impact of GCN2 on the myeloid phenotype and immune responses in melanoma. We found that myeloid-lineage deletion of GCN2 drives a shift in the phenotype of tumor-associated macrophages and myeloid-derived suppressor cells (MDSCs) that promotes antitumor immunity. Time-of-flight mass cytometry (CyTOF) and single-cell RNA sequencing showed that this was due to changes in the immune microenvironment with increased proinflammatory activation of macrophages and MDSCs and interferon-γ expression in intratumoral CD8 T cells. Mechanistically, GCN2 altered myeloid function by promoting increased translation of the transcription factor CREB-2/ATF4, which was required for maturation and polarization of macrophages and MDSCs in both mice and humans, whereas targeting by small interfering RNA knockdown reduced tumor growth. Last, analysis of patients with cutaneous melanoma showed that GCN2-dependent transcriptional signatures correlated with macrophage polarization, T cell infiltrates, and overall survival. Thus, these data reveal a previously unknown dependence of tumors on myeloid GCN2 signals for protection from immune attack.
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http://dx.doi.org/10.1126/sciimmunol.aax8189DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7201901PMC
December 2019

RNPS1 inhibition aggravates ischemic brain injury and promotes neuronal death.

Biochem Biophys Res Commun 2020 02 9;523(1):39-45. Epub 2019 Dec 9.

Department of Neurology, Drum Tower Hospital, Medical School and The State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, 210008, China; Jiangsu Key Laboratory for Molecular Medicine, Medical School of Nanjing University, Nanjing, 210093, China. Electronic address:

RNA-binding protein with serine-rich domain 1 (RNPS1) is essential for modulating mRNA metabolism, but its role in ischemic stroke is unknown. In this study, we found that RNPS1 expression was significantly up-regulated in the brains of ischemic stroke mice and primary cortical neurons after oxygen-glucose deprivation (OGD) treatment. Knockdown of RNPS1 significantly aggravated ischemic brain injury after middle cerebral artery occlusion (MCAO) and promoted neuronal death. In addition, knockdown of RNPS1 exacerbated ischemia induced neuronal apoptosis, and downregulated the expression of anti-apoptotic proteins Bcl-xL and Mcl-1. Our study suggested that RNPS1 might be a potential therapeutic target for alleviating neuronal death in ischemic stroke.
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http://dx.doi.org/10.1016/j.bbrc.2019.11.185DOI Listing
February 2020

Acetone Dimer Hydrogenation under Vacuum Ultraviolet: An Intracluster Trimolecular Dissociation Mechanism.

J Phys Chem A 2019 Dec 9;123(50):10739-10745. Epub 2019 Dec 9.

Beijing National Laboratory for Molecular Sciences (BNLMS), State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry , Chinese Academy of Sciences , Beijing 100190 , P. R. China.

Hydrogenation of organic chemicals is one of the most frequent things that people take for granted in mass spectroscopy; however, it could provide important information on spontaneous or stimulated hydrogen transfer in initiating chemical reactions and in determining the product selectivity and conversion efficiency. Here, we present a study of hydrogenation of acetone via vacuum ultraviolet laser ionization mass spectrometry (VUV-LIMS) and density functional theory (DFT) calculations. It is interestingly found that acetone dimer readily captures a hydrogen to form (CHO)H in the presence of alcohols, shedding light on the intracluster hydrogen atom transfer via a trimolecular mechanism. This is well consistent with the DFT calculation results of energetics and reaction kinetics. It is worth noting that, although the hydrogen bond interaction of O-H···O is stronger than that of C-H···O, the hydrogen atom transfer (HAT) tends to proceed from the methyl group of the alcohols to acetone. We fully demonstrate the intracluster HAT reactivity of such a simple system and provide new insights into hydrogen bond interactions and molecular cluster chemistry.
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http://dx.doi.org/10.1021/acs.jpca.9b08833DOI Listing
December 2019

Age and regional disparity in HIV education among migrants in China: migrants population dynamic monitoring survey, 2014-2015.

Int J Equity Health 2019 07 3;18(1):104. Epub 2019 Jul 3.

Rory Meyers College of Nursing, New York University, New York, 10010, USA.

Objective: A lack of education among migrants remains an important but overlooked issue that indirectly contributes to HIV transmission. It is necessary to know who has received HIV education and who has a lower probability of being educated among migrants across different regions and age groups in China.

Methods: We used pooled data from the 2014 and 2015 Migrants Population Dynamic Monitoring Survey. The study population included 406,937 Chinese migrants. Participants were asked whether they had received any HIV education after migrating to the destination city. Regions were categorized into east-coast, central, northwest, southwest, west-Tibet, west-Uyghur, and northeast regions. Hierarchical logistic regression modeling was conducted to investigate the relationships between the independent variables and HIV education.

Results: Of 406,937 participants, half (50.6%) had reported receiving HIV education. Individuals in the west-Uyghur region had the highest proportion of receiving HIV education (73.0%), followed by the southwest region (67.9%) and the west-Tibet region (54.8%). Methods of receiving HIV education varied among different age groups. Individuals who were in a region with a higher prevalence of HIV, a lower density of medical professionals, and a higher density of migrants were more likely to receive HIV education.

Conclusions: The study showed significant regional disparities among migrants in China. More HIV resources need to be allocated to regions with large-scale floating populations, such as the east-coast region. Providing multiple options, including both new and traditional media, for both young and elderly migrants is essential. HIV education should be tailored to the age of migrants with low educational and income levels.
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http://dx.doi.org/10.1186/s12939-019-0999-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6610800PMC
July 2019

Validation of CyTOF Against Flow Cytometry for Immunological Studies and Monitoring of Human Cancer Clinical Trials.

Front Oncol 2019 17;9:415. Epub 2019 May 17.

Tumor Immunology Program, Princess Margaret Cancer Center, University Health Network, Toronto, ON, Canada.

Flow cytometry is a widely applied approach for exploratory immune profiling and biomarker discovery in cancer and other diseases. However, flow cytometry is limited by the number of parameters that can be simultaneously analyzed, severely restricting its utility. Recently, the advent of mass cytometry (CyTOF) has enabled high dimensional and unbiased examination of the immune system, allowing simultaneous interrogation of a large number of parameters. This is important for deep interrogation of immune responses and particularly when sample sizes are limited (such as in tumors). Our goal was to compare the accuracy and reproducibility of CyTOF against flow cytometry as a reliable analytic tool for human PBMC and tumor tissues for cancer clinical trials. We developed a 40+ parameter CyTOF panel and demonstrate that compared to flow cytometry, CyTOF yields analogous quantification of cell lineages in conjunction with markers of cell differentiation, function, activation, and exhaustion for use with fresh and viably frozen PBMC or tumor tissues. Further, we provide a protocol that enables reliable quantification by CyTOF down to low numbers of input human cells, an approach that is particularly important when cell numbers are limiting. Thus, we validate CyTOF as an accurate approach to perform high dimensional analysis in human tumor tissue and to utilize low cell numbers for subsequent immunologic studies and cancer clinical trials.
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http://dx.doi.org/10.3389/fonc.2019.00415DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6534060PMC
May 2019

Severe Fever With Thrombocytopenia Syndrome Virus-Induced Macrophage Differentiation Is Regulated by miR-146.

Front Immunol 2019 15;10:1095. Epub 2019 May 15.

Center for Public Health Research, Medical School, Nanjing University, Nanjing, China.

Severe fever with thrombocytopenia syndrome (SFTS) is an emerging hemorrhagic fever with a high mortality rate in humans, which is caused by SFTS virus (SFTSV), a novel phlebovirus in the family, is tick borne and endemic in Eastern Asia. Previous study found that SFTSV can infect and replicate in macrophages and . However, the role of macrophages in virus replication and the potential pathogenic mechanisms of SFTSV in macrophage remain unclear. In this study, we provided evidence that the SFTSV infection drove macrophage differentiation skewed to M2 phenotype, facilitated virus shedding, and resulted in viral spread. We showed evidence that miR-146a and b were significantly upregulated in macrophages during the SFTSV infection, driving the differentiation of macrophages into M2 cells by targeting STAT1. Further analysis revealed that the elevated miR-146b but not miR-146a was responsible for IL-10 stimulation. We also found that SFTSV increased endogenous miR-146b-induced differentiation of macrophages into M2 cells mediated by viral non-structural protein (NSs). The M2 skewed differentiation of macrophages may have important implication to the pathogenesis of SFTS.
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http://dx.doi.org/10.3389/fimmu.2019.01095DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6529556PMC
September 2020

Association between Dying Experience and Place of Death: Urban-Rural Differences among Older Chinese Adults.

J Palliat Med 2019 11 23;22(11):1386-1393. Epub 2019 May 23.

Rory Meyers College of Nursing and NYU Aging Incubator, New York University, New York, New York.

The quality of the dying experience among older adults should improve with a better understanding of the dying experience and its association with the place of death in Mainland China. This study investigated the relationship between the dying experience and place of death among older Chinese adults in the context of an urban-rural bifurcated system. We used the end-of-life module data from the China Longitudinal Aging Social Survey conducted in 2014 and 2016 with an eligible sample of 352 decedents ages 60 and older. Facial expression and sadness at the end of life were indicators of the dying experience in the present study. We performed multiple regression models to examine the association between the place of death and dying experience after adjusting for an ecological array of factors at the individual, family, and community levels. The urban-rural differences in the association between facial expression at death and place of death were identified (interaction term:  = 0.16,  = 0.004). Among the decedents with a rural residence status, dying in a hospital was associated with a more peaceful facial expression at death than dying at home ( < 0.001). Among the decedents with an urban residence status, the place of death was not significantly related to the dying experience. Although home is perceived as a common place for death, the findings revealed that dying at home was less positive for rural older adults compared with dying in hospital. Bridging the gaps between urban and rural areas is necessary for the reform and construction of health care and long-term care systems in China.
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http://dx.doi.org/10.1089/jpm.2018.0583DOI Listing
November 2019

Perceived discrimination and cognitive function in middle-aged and older adults living with HIV in China.

AIDS Care 2019 09 3;31(9):1061-1068. Epub 2019 Apr 3.

d NYU Rory Meyers College of Nursing , New York , USA.

Middle-aged and older adults with HIV experience double discrimination and cognitive impairment due to both their HIV status and their age. However, the relationship between perceived discrimination and self-reported cognitive ability in middle-aged and older people living with HIV (PLWH) is less clear. We measured self-reported perceived discrimination and cognitive ability using and the subscale of . The study sample included 324 middle-aged and older PLWH (over 45 years old) from five designated HIV hospitals in three regions (east coast, middle, and southwest regions) of China. The descriptive analysis showed that 45.37% of the participants reported perceiving discrimination at least once in the past twelve months, and 47.22% reported having at least one type of cognitive impairment. Multiple linear regression results showed that higher levels of perceived discrimination (= -0.121, = 0.036) were significantly associated with lower levels of self-reported cognitive ability after controlling for several covariates, including sociodemographic variables, mental health status, health behaviors, and social support. A longer duration of HIV was also related to a lower level of self-reported cognitive ability. Our findings indicate that perceived discrimination is related to self-reported cognitive ability and suggest that counseling services and support systems should be developed to reduce age- and disease-associated discrimination. A reduction in perceived discrimination would improve not only overall wellbeing but also cognitive ability in later life.
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http://dx.doi.org/10.1080/09540121.2019.1601674DOI Listing
September 2019

An interim report on the investigator-initiated phase 2 study of pembrolizumab immunological response evaluation (INSPIRE).

J Immunother Cancer 2019 03 13;7(1):72. Epub 2019 Mar 13.

Princess Margaret Cancer Centre, University Health Network, Toronto, Canada.

Background: Immune checkpoint inhibitors (ICIs) demonstrate unprecedented efficacy in multiple malignancies; however, the mechanisms of sensitivity and resistance are poorly understood and predictive biomarkers are scarce. INSPIRE is a phase 2 basket study to evaluate the genomic and immune landscapes of peripheral blood and tumors following pembrolizumab treatment.

Methods: Patients with incurable, locally advanced or metastatic solid tumors that have progressed on standard therapy, or for whom no standard therapy exists or standard therapy was not deemed appropriate, received 200 mg pembrolizumab intravenously every three weeks. Blood and tissue samples were collected at baseline, during treatment, and at progression. One core biopsy was used for immunohistochemistry and the remaining cores were pooled and divided for genomic and immune analyses. Univariable analysis of clinical, genomic, and immunophenotyping parameters was conducted to evaluate associations with treatment response in this exploratory analysis.

Results: Eighty patients were enrolled from March 21, 2016 to June 1, 2017, and 129 tumor and 382 blood samples were collected. Immune biomarkers were significantly different between the blood and tissue. T cell PD-1 was blocked (≥98%) in the blood of all patients by the third week of treatment. In the tumor, 5/11 (45%) and 11/14 (79%) patients had T cell surface PD-1 occupance at weeks six and nine, respectively. The proportion of genome copy number alterations and abundance of intratumoral 4-1BB+ PD-1+ CD8 T cells at baseline (P < 0.05), and fold-expansion of intratumoral CD8 T cells from baseline to cycle 2-3 (P < 0.05) were associated with treatment response.

Conclusion: This study provides technical feasibility data for correlative studies. Tissue biopsies provide distinct data from the blood and may predict response to pembrolizumab.
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http://dx.doi.org/10.1186/s40425-019-0541-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6417194PMC
March 2019

Nursing students' experiences with faculty incivility in the clinical education context: a qualitative systematic review and meta-synthesis.

BMJ Open 2019 03 1;9(2):e024383. Epub 2019 Mar 1.

School of Nursing, Fudan University, Shanghai, China.

Objective: The aim of this study is to synthesise evidence for the experiences and perceptions of incivility during clinical education of nursing students.

Design: We used a meta-aggregation approach to conduct a systematic review of qualitative studies.

Data Sources: Published and unpublished papers from 1990 until 13 January 2018 were searched using electronic databases, including CINAHL, PubMed (MEDLINE), ProQuest Central, ProQuest Education Journals, ProQuest XML-Dissertations and Theses, Web of Science, Embase, EBSCO Discovery Service and PsycINFO. The search for unpublished studies included the Open Grey collection, conference proceedings and the Deep Blue Library.

Eligibility Criteria: We included qualitative studies that focused on nursing students' perceptions and experiences of incivility from faculty during their clinical education.

Data Extraction And Synthesis: Two reviewers independently appraised the methodological quality and extracted relevant data from each included study. Meta-aggregation was used to synthesise the data.

Results: A total of 3397 studies was returned from the search strategies. Eighteen studies fulfilled the inclusion criteria and were included in the meta-synthesis. Six synthesised findings were identified, covering features of incivility, manifestations of incivility, contributing factors, impacts on students, coping strategies and suggestions.

Conclusions: The results showed experiences of incivility during clinical education. However, the confidence was low for all synthesised findings. We suggest that nursing students should try to cope positively with incivility. Nurse managers and clinical preceptors should be aware of the prevalence and impact of incivility and implement policies and strategies to reduce incivility towards nursing students. Hospitals and universities should have an immediate response person or system to help nursing students confronting incivility and create an open communication environment.
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http://dx.doi.org/10.1136/bmjopen-2018-024383DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6398780PMC
March 2019

Urban and Rural Differences: Unmet Needs for Symptom Management in People Living With HIV in China.

J Assoc Nurses AIDS Care 2019 Mar-Apr;30(2):206-217

Zheng Zhu, MSN, RN, is a Doctoral Candidate, School of Nursing, Fudan University, Shanghai, China, and is a core researcher of Fudan University Centre for Evidence-based Nursing: A Joanna Briggs Institute Centre of Excellence, Shanghai, China. Yan Hu, PhD, RN, FAAN, is a Professor and Dean, School of Nursing, Fudan University, Shanghai, China, and is a Director of Fudan University Centre for Evidence-based Nursing: A Joanna Briggs Institute Centre of Excellence, Shanghai, China. Mengdi Guo, JM, is a Doctoral Candidate, School of Public Affairs, Zhejiang University, Hangzhou, China. Ann Bartley Williams, ACRN, EdD, FAAN, is Faculty and Director of Global Initiatives, New York University, Rory Meyers College of Nursing, New York City, New York, USA.

Access to HIV care has increased in China, but regional disparities for the unmet symptom management needs of people living with HIV remain. Little attention has been paid to these disparities, especially in rural areas. We describe unmet symptom management needs in 1,214 people living with HIV from five HIV hospitals in the eastern coast, middle, and southern regions of China. Insomnia, abnormal dreams, and fatigue were the most common unmet needs of patients in urban China, whereas cognitive problems and blurry vision were the most common in rural China. Self-management capacity, discrimination, and symptom severity predicted more unmet symptom management needs for patients in urban areas. Medication adherence, basic function, and symptom severity predicted more unmet needs among patients in rural areas. Our results have implications for nurses in HIV care and HIV policymakers. Separate interventions targeting the urban and rural areas must be implemented to meet patient symptom management needs.
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http://dx.doi.org/10.1097/JNC.0000000000000025DOI Listing
June 2020

Selective C-C and C-N bond activation in dopamine and norepinephrine under deep ultraviolet laser irradiation.

Chem Commun (Camb) 2019 Apr;55(28):4015-4018

State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China.

Utilizing a customized mass spectrometer, we analyze selective C-C bond and C-N bond activation in neurotransmitters dopamine (DA) and norepinephrine (NE) under deep ultraviolet laser irradiation.
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http://dx.doi.org/10.1039/c9cc00444kDOI Listing
April 2019

Identifying Symptom Clusters Among People Living With HIV on Antiretroviral Therapy in China: A Network Analysis.

J Pain Symptom Manage 2019 03 20;57(3):617-626. Epub 2018 Nov 20.

NYU Rory Meyers College of Nursing, New York City, New York, USA.

Context: There exists a research interest shift from separate symptoms to symptom clusters among people living with HIV (PLWH), which may provide a better understanding of symptom management in HIV/AIDS care. However, the symptom clusters among Chinese PLWH are still unknown.

Objectives: The aim of our study was to identify symptom clusters and to examine demographic and health-related factors associated with these symptom clusters among PLWH prescribing antiretroviral therapy (ART) in China.

Methods: From April to September 2017, we recruited 1116 participants through a convenience sampling in five HIV/AIDS designated facilities in the eastern, middle, and southwest regions of China. The principal component analysis was used to identify the symptom clusters. Association network was adopted to describe the relationships among symptoms and clusters. A multiple linear model was used to investigate the associated factors for the severity of overall symptoms and the prevalence of each symptom clusters.

Results: Five symptom clusters were identified, including cognitive dysfunction, mood disturbance, wasting syndrome, dizziness/headache, and skin-muscle-joint disorder. Cognitive dysfunction was the most central symptom cluster. Variables including primary caregiver during ART treatment, years of HIV diagnosis and ART use, having comorbidity, self-rated health, and quality of life were associated with the prevalence of these five symptom clusters.

Conclusion: Our study suggests that there is a need to evaluate symptom clusters for the improvement of symptom management among PLWH. It is particularly important to include assessment and treatment of cognitive symptoms as an essential component of the HIV care.
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http://dx.doi.org/10.1016/j.jpainsymman.2018.11.011DOI Listing
March 2019

Cutting Edge: Evidence for Nonvascular Route of Visceral Organ Immunosurveillance by T Cells.

J Immunol 2018 07 6;201(2):337-342. Epub 2018 Jun 6.

Department of Microbiology and Immunology, Center for Immunology, University of Minnesota, Minneapolis, MN 55455; and

Lymphocytes enter tissues from blood vessels through a well-characterized three-step process of extravasation. To our knowledge, nonvascular routes of lymphocyte entry have not been described. In this article, we report that Ag-experienced CD8 T cells in mice recirculate from blood through the peritoneal cavity. In the event of infection, Ag-experienced CD8 T cell subsets adhered to visceral organs, indicating potential transcapsular immunosurveillance. Focusing on the male genital tract (MGT), we observed Ag-experienced CD8 T cell migration from the peritoneal cavity directly to the infected MGT across the capsule, which was dependent on the extracellular matrix receptor CD44. We also observed that, following clearance of infection, the MGT retained functional resident memory CD8 T cells. These data suggest that recirculation through body cavities may provide T cells with opportunities for broad immunosurveillance and potential nonvascular mechanisms of entry.
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http://dx.doi.org/10.4049/jimmunol.1800279DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6039241PMC
July 2018
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