Publications by authors named "Meng-Ying Zhang"

18 Publications

  • Page 1 of 1

A highly conductive self-assembled multilayer graphene nanosheet film for electronic tattoos in the applications of human electrophysiology and strain sensing.

Nanoscale 2021 Jun 9;13(24):10798-10806. Epub 2021 Jun 9.

School of Mechanical and Materials Engineering, North China University of Technology, Beijing 100144, China.

Highly conductive, conformable and gel-free electrodes are desirable in human electrophysiology. Besides, intimately coupling with human skin, wearable strain sensors can detect numerous physiological signals, such as wrist pulse and breath. In this study, a multilayer graphene nanosheet film (MGNF) with high conductivity was prepared by the Marangoni self-assembly for using in tattoo dry electrodes (TDEs) and in a graphene tattoo strain sensor (GTSS). Compared to commercial Ag/AgCl gel electrodes, TDEs have lower skin-electrode contact impedance and could detect human electrocardiogram for 24-hour wearing more accurately as well as electromyogram. Through designing a slim serpentine ribbon structure, a resistance-type GTSS, without deterioration even after 2000 cycles, is well demonstrated for human wrist pulse and breath sensing. With the advantages of high conductivity and conformability, MGNF provides support to fabricate low-cost, customizable, and high-performance electronic tattoos for human electrophysiology and strain sensing.
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http://dx.doi.org/10.1039/d0nr08032bDOI Listing
June 2021

Anti-inflammatory effects of Rhodiola rosea L.: A review.

Biomed Pharmacother 2020 Jan 9;121:109552. Epub 2019 Nov 9.

College of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China.

Rhodiola rosea L., a worldwide botanical adaptogen, has been confirmed to possess protective effects of inflammatory injury for many diseases, including cardiovascular diseases, neurodegenerative diseases, diabetes, sepsis, and cancer. This paper is to review the recent clinical and experimental researches about the anti-inflammatory effects and the related mechanisms of Rhodiola rosea L. extracts, preparations, and the active compounds. From the collected information reviewed, this paper will provide the theoretical basis for its clinical application, and provide the evidences or guidance for future studies and medicinal exploitations of Rhodiola rosea L.
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http://dx.doi.org/10.1016/j.biopha.2019.109552DOI Listing
January 2020

Baicalein attenuates pancreatic inflammatory injury through regulating MAPK, STAT 3 and NF-κB activation.

Int Immunopharmacol 2019 Jul 15;72:204-210. Epub 2019 Apr 15.

Tianjin Key Laboratory of Chinese Medicine Pharmacology, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China; School of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China. Electronic address:

Acute pancreatitis (AP) is a common acute abdominal disease with local or systemic inflammatory response, caused by abnormal activation of digestive enzymes. Baicalein has been shown to exert anti-inflammatory effects and to attenuate the pathological changes of AP. The aim of the research was to investigate the effects of baicalein on caerulein induced pancreatitis, and to elucidate the putative underlying mechanism. In this study, the therapeutic potential of baicalein and its mechanism were investigated in a caerulein-induced AP in vivo and in vitro model. The results indicate that baicalein treatment alleviates the caerulein-induced pathological damage in the pancreas. Baicalein decreased the expression level of pro-inflammatory cytokines and chemokines of the pancreas in caerulein treated mice and of isolated pancreatic acinar cells. Moreover, baicalein inhibited the expression of NF-κB p65 and the phosphorylation of p38 MAPK, ERK (extracellular signal-regulated kinase) as well as STAT 3, which indicates that baicalein exerts its anti-inflammatory effects via dampening the NF-κB, MAPK and STAT 3 signaling pathways. Together, this study provides experimental evidence for the clinical application of Scutellaria baicalensis Georgi or baicalein and indicates that baicalein may be a promising candidate for treatment of AP patients in the future.
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http://dx.doi.org/10.1016/j.intimp.2019.04.018DOI Listing
July 2019

Impact of Enterprise Ownership and Size on Registered Hematotoxicity in Benzene-exposed Workers.

Biomed Environ Sci 2018 Dec;31(12):917-921

Key Laboratory of Environmental Medicine Engineering of Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, Jiangsu, China.

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http://dx.doi.org/10.3967/bes2018.125DOI Listing
December 2018

Gamma Oscillation Dysfunction in mPFC Leads to Social Deficits in Neuroligin 3 R451C Knockin Mice.

Neuron 2018 03 1;97(6):1253-1260.e7. Epub 2018 Mar 1.

Department of Neurobiology, Key Laboratory of Medical Neurobiology of the Ministry of Health of China, Collaborative Innovation Center for Brain Science, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China. Electronic address:

Neuroligins (NLs) are critical for synapse formation and function. NL3 R451C is an autism-associated mutation. NL3 R451C knockin (KI) mice exhibit autistic behavioral abnormalities, including social novelty deficits. However, neither the brain regions involved in social novelty nor the underlying mechanisms are clearly understood. Here, we found decreased excitability of fast-spiking interneurons and dysfunction of gamma oscillation in the medial prefrontal cortex (mPFC), which contributed to the social novelty deficit in the KI mice. Neuronal firing rates and phase-coding abnormalities were also detected in the KI mice during social interactions. Interestingly, optogenetic stimulation of parvalbumin interneurons in the mPFC at 40 Hz nested at 8 Hz positively modulated the social behaviors of mice and rescued the social novelty deficit in the KI mice. Our findings suggest that gamma oscillation dysfunction in the mPFC leads to social deficits in autism, and manipulating mPFC PV interneurons may reverse the deficits in adulthood.
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http://dx.doi.org/10.1016/j.neuron.2018.02.001DOI Listing
March 2018

[Expression and Clinical Significance of N-cadherin in Bone Marrow Leukemic Cells Derived from Patients with Acute Leukemia].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2016 Oct;24(5):1312-1318

Department of Hematology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China.

Objective: To investigate the expression of N-cadherin in bone marrow leukemic cells derived from acute leukemia patients and its clinical significances.

Methods: A total of 113 patients with acute leukemia were enrolled in this study. Flow cytometry was employed to detect the expression of N-Cadherin in bone marrow leukemic cells from acute leukemia patients and the relationships between the N-cadherin expression and the clinical characteristics of patients with acute leukemia were analyzed.

Results: The expression of N-Cadherin in bone marrow leukemic cells deriveted from patients with acute leukemia was variable with 0%-99.7%. For adult AML patients, the positive rate of CD34 in N-cadherin group was significantly higher than that in N-cadherin group(67.39% vs 33.33%)(P=0.013), while the differences of total CR rate and rate of CR after 1 cycle of induction treatment were not significant between these 2 groups(P>0.05). As to ALL patients, N-cadherin group had significant lower WBC count (21.31±7.07 vs 51.10±23.69)(P=0.008) and lower percentage of peripheral blood blast (43.22±5.75% vs 66.45±5.65%)(P=0.015). The CR rate after 1 cycle of induction treatment and rate of overall CR were lower and the relapse rate was higher in N-cadherin ALL group than those in N-cadherin ALL group, but the differences were not significant (P>0.05). For childhood ALL, the positive rate of CD33 in N-cadherin group was significantly higher than that in N-cadherin group(47.62% vs 0%)(P=0.012). The relapse rate was higher in N-cadherin group than that in N-cadherin group (30.00% vs 0%)(P=0.115). The median survival time, 3-year overall OS rate and 3-year relapse-free survival rate in N-cadherin groups of adult AML, non-M3 AML, ALL and chidhood ALL paients were superior to N-cadherin groups, but the differences were not significant.

Conclusion: The expression of N-cadherin in bone marrow leukemic cells relates to some clinical features of patients with acute leukemia and to some extent has inferior effect on survival of patients with acute leukemia.
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http://dx.doi.org/10.7534/j.issn.1009-2137.2016.05.005DOI Listing
October 2016

[Expression and Clinical Significance of N-cadherin in Bone Marrow Leukemic Cells Derived from Patients with Acute Leukemia].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2016 Oct;24(5):1312-1318

Department of Hematology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China.

Objective: To investigate the expression of N-cadherin in bone marrow leukemic cells derived from acute leukemia patients and its clinical significances.

Methods: A total of 113 patients with acute leukemia were enrolled in this study. Flow cytometry was employed to detect the expression of N-Cadherin in bone marrow leukemic cells from acute leukemia patients and the relationships between the N-cadherin expression and the clinical characteristics of patients with acute leukemia were analyzed.

Results: The expression of N-Cadherin in bone marrow leukemic cells deriveted from patients with acute leukemia was variable with 0%-99.7%. For adult AML patients, the positive rate of CD34 in N-cadherin group was significantly higher than that in N-cadherin group(67.39% vs 33.33%)(P=0.013), while the differences of total CR rate and rate of CR after 1 cycle of induction treatment were not significant between these 2 groups(P>0.05). As to ALL patients, N-cadherin group had significant lower WBC count (21.31±7.07 vs 51.10±23.69)(P=0.008) and lower percentage of peripheral blood blast (43.22±5.75% vs 66.45±5.65%)(P=0.015). The CR rate after 1 cycle of induction treatment and rate of overall CR were lower and the relapse rate was higher in N-cadherin ALL group than those in N-cadherin ALL group, but the differences were not significant (P>0.05). For childhood ALL, the positive rate of CD33 in N-cadherin group was significantly higher than that in N-cadherin group(47.62% vs 0%)(P=0.012). The relapse rate was higher in N-cadherin group than that in N-cadherin group (30.00% vs 0%)(P=0.115). The median survival time, 3-year overall OS rate and 3-year relapse-free survival rate in N-cadherin groups of adult AML, non-M3 AML, ALL and chidhood ALL paients were superior to N-cadherin groups, but the differences were not significant.

Conclusion: The expression of N-cadherin in bone marrow leukemic cells relates to some clinical features of patients with acute leukemia and to some extent has inferior effect on survival of patients with acute leukemia.
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http://dx.doi.org/10.7534/j.issn.1009-2137.2016.05.005DOI Listing
October 2016

[Peripheral blood lymphocyte subsets and their relation with early response to treatment in patients with low or intermediate risk myelodysplastic syndrome (IPSS score ≤ 1.0) and chronic aplastic anemia].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2014 Apr;22(2):377-81

Department of Hematology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China.

The purpose of this study was retrospectively to analyze the peripheral blood lymphocyte subset distribution in patients with low or intermediate risk myelodysplastic syndromes (IPSS ≤ 1.0) and chronic aplastic anemia (CAA), and their hematological changes of peripheral blood after treatment, so as to understand differences and their relation with early treatment response. The lymphocyte subsets in peripheral blood of 67 patient with low or intermediate risk MDS (IPSS ≤ 1.0), 54 patients with CAA and 73 healthy individuals were analyzed by flow cytometry. The results showed that Th cells, Th/Ts ratio in peripheral blood of low or intermediate risk MDS were 42.94% ± 10.80% and 1.80% ± 0.99% respectively, and were significantly higher than those in control group; the CD16(+) CD56(+) cell ratio was 11.22% ± 7.97%, and was significantly lower than that in control group, the difference was statistically significant (P < 0.05); Ts cells and CD19(+) cell ratio in peripheral blood of CAA patients were 30.87% ± 9.11% and 16.98% ± 7.40% respectively, and were significantly higher than those in control group; CD16(+) CD56(+) cell ratio was 9.81% ± 7.00%, and was significantly lower than that in normal control group, and the difference was statistically significant (P < 0.05); while the Th cells and Th/Ts ratio in low or intermediate risk MDS group were significantly higher than those in CAA group, and the difference was statistically significant (P < 0.05). After treatment for 6 mouths, the HI-E and HI-N rates in CD19(+) cell normal group of low or intermediate risk MDS patients were 18.2% (4/22) and 13.6% (3/22), and were significantly lower than that in the increased group and decreased group. In Ts cell increased group HI-N rate was 15.4% (2/13), and was significantly lower than that in normal group and decreased group. In Th/Ts ratio decreased group HI-N rate was 14.3% (2/14), and was significantly lower than that in the increase group and normal group, the difference was statistically significant (P < 0.05). After treatment of CAA for 6 months, the effective rate for CD3(+) cells, Th cells, Th/Ts ratio in decreased group was 71.4% (5/7), 56.3% (9/16), 50.0% (10/20), and were significantly higher than those in increased and normal group, and the difference was statistically significant (P < 0.05). It is concluded that the peripheral blood lymphocyte subsets of low or intermediate risk MDS(IPSS score ≤ 1.0) and CAA are abnormal, and these lymphocyte subsets are related with hematologic changes after early response to treatment.
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http://dx.doi.org/10.7534/j.issn.1009-2137.2014.02.020DOI Listing
April 2014

Enhancement of second-order nonlinear optical response in boron nitride nanocone: Li-doped effect.

J Mol Graph Model 2014 Mar 23;48:28-35. Epub 2013 Sep 23.

Institute of Functional Material Chemistry, Faculty of Chemistry, Northeast Normal University, Changchun 130024, Jilin, People's Republic of China. Electronic address:

The unusual properties of Li-doped boron nitride nanomaterials have been paid further attention due to their wide applications in many promising fields. Here, density functional theory (DFT) calculations have been carried out to investigate the second-order nonlinear optical (NLO) properties of boron nitride nanocone (BNNC) and its Li-doped BNNC derivatives. The natural bond orbital charge, electron location function, localized orbital locator and frontier molecular orbital analysis offer further insights into the electron density of the Li-doped BNNC derivatives. The electron density is effectively bounded by the Li atom and its neighboring B atoms. The Li-doped BNNC molecules exhibit large static first hyperpolarizabilities (β(tot)) up to 1.19×10³ a.u. for [email protected], 5.05×10³ a.u. for [email protected], and 1.08×10³ a.u. for [email protected], which are significantly larger than that of the non-doped BNNC (1.07×10² a.u.). The further investigations show that there are clearly dependencies of the first hyperpolarizabilities on the transition energies and oscillator strengths. Moreover, time-dependent DFT results show that the charge transfer from BNNC to Li atom becomes more pronounced as doping the Li atom to BNNC. It is also found that the frequency-dependent effect on the first hyperpolarizabilities is weak, which may be beneficial to experimentalists for designing Li-doped BNNC molecules with large NLO responses.
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http://dx.doi.org/10.1016/j.jmgm.2013.09.008DOI Listing
March 2014

[Autophagy expression and its significance in chondrocytes from a degenerate model of human cervical endplate].

Zhonghua Yi Xue Za Zhi 2013 Aug;93(31):2474-7

Department of Orthopedic Surgery, Yijishan Hospital, Wannan Medical College, Wuhu 241001, China.

Objective: To explore the autophagy expression and examine its significance in chondrocytes in a degenerate model of human cervical vertebrae endplate.

Methods: Cartilage endplates were obtained from 48 hospitalized patients with cervical vertebral fracture or dislocation at our hospital between February 2012 to August 2012. They were divided into cervical spondylosis group with cervical spondylotic myelopathy (n = 31) and control group (n = 17).Endplate chondrocytes were isolated by enzyme digestion and cultured in vitro. The cells were stained with toluidine blue and hematoxylin and eosin; laser scanning confocal microscope and monodansylcadaverine (MDC) were used to observe autophagy in endplate chondrocytes; reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the mRNA expression of typeIIcollagen and aggrecan and Western blot for the protein of LC3.

Results: A degenerative cell model of human cervical endplate chondrocytes was established successfully in vitro. Compared with the common group, the cellular morphologies of degenerative group showed spindle changes. Autophagic body was stained with MDC.Intracellular and perinuclear LC3 protein was detected by laser confocal microscopy. Compared with the control group, the mRNA expressions of aggrecan (0.715 ± 0.194) and typeII collagen (0.628 ± 0.254) markedly decreased (0.845 ± 0.186,0.913 ± 0.254, P < 0.05) and LC3-II/LC3-I declined in cervical spondylosis group.

Conclusion: Autophagy plays an important pathogenic role in the process of human cervical disc degeneration. And regulating its expression may improve disc degeneration in endplate cartilage cells.
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August 2013

Computational investigation on redox-switchable nonlinear optical properties of a series of polycyclic p-quinodimethane molecules.

J Mol Model 2013 Dec 17;19(12):5479-87. Epub 2013 Nov 17.

College Chemistry & Chemical Engineering, Hainan Normal University, Haikou, Hainan, 571158, People's Republic of China,

The polycyclic p-quinodimethanes are proposed to be the novel candidates of the high-performance nonlinear optical (NLO) materials because of their large third order polarizabilities (γ). We investigate the switchable NLO responses of a series of polycyclic p-quinodimethanes with redox properties by employing the density functional theory (DFT). The polycyclic p-quinodimethanes are forecasted to exhibit obvious pure diradical characters because of their large y 0 index (the y 0 index is a value between 0 [closed-shell state] and 1 [pure biradical state]). The γ values of these polycyclic p-quinodimethanes and their corresponding one-electron and two-electron reduced/oxidized species are calculated by the (U)BHandHLYP method. The γ values of polycyclic p-quinodimethanes and their corresponding one-electron reduced species are all positive and significantly different. The large differences of the γ values are due to a change in the transition energy and are related to the different delocalization of the spin density, which demonstrates that the NLO switching is more effective on one-electron reduction reactions. Therefore, the study on these polycyclic p-quinodimethanes provides a guideline for a molecular design of highly efficient NLO switching.
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http://dx.doi.org/10.1007/s00894-013-2035-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3851701PMC
December 2013

Strategy for enhancing second-order nonlinear optical properties of the Pt(II) dithienylethene complexes: substituent effect, π-conjugated influence, and photoisomerization switch.

J Phys Chem A 2013 Nov 13;117(47):12497-510. Epub 2013 Nov 13.

Institute of Functional Material Chemistry, Faculty of Chemistry, Northeast Normal University , Changchun 130024, Jilin, People's Republic of China.

The second-order nonlinear optical (NLO) properties of a series of Pt(II) dithienylethene (DTE) complexes possessing the reversible photochromic behavior have been investigated by density functional theory (DFT) combined with the analytic derivatives method. The results show that the calculated static first hyperpolarizabilities (βtot) of the open-ring and closed-ring systems significantly increase in the range of 2.1-4.5 times through strengthening of the electron-withdrawing ability of the substituent R (R = H, CF3, NO2) and an increase of the number of thiophene rings. Moreover, there is a large enhancement of the βtot values from the open-ring systems to the corresponding closed-ring systems. This efficient enhancement is attributed to the better delocalization of the π-electron system, the more obvious degree of charge transfer, and the larger f(os)/E(gm)(3) (f(os) is the oscillator strength, and E(gm) is the transition energy between the ground and the excited states) values in the closed forms according to the bond length alternation (BLA) and time-dependent density functional theory (TDDFT) calculations. In addition, the dispersion has less influence on the frequency-dependent first hyperpolarizabilities (βtot(ω)) of the studied systems at the low-frequency area ω (0.000-0.040 au). Our present work would be beneficial for further theoretical and experimental studies on large second-order NLO responses of metal complexes.
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http://dx.doi.org/10.1021/jp4041265DOI Listing
November 2013

Third-order nonlinear optical properties of molecules containing aromatic diimides: effects of the aromatic core size and a redox-switchable modification.

J Mol Graph Model 2013 Apr 16;41:79-88. Epub 2013 Feb 16.

College of Chemistry & Chemical Engineering, Hainan Normal University, Haikou 571158, People's Republic of China.

The third-order nonlinear optical (NLO) properties of aromatic diimide molecules have been studied for the first time using density functional theory (DFT) with a finite field (FF). This study shows that the size of the aromatic core can affect the static second hyperpolarizability (γ). Increasing the number of benzenes along the longitudinal axis can effectively improve the γ values because the degree of charge transfer along the longitudinal direction increases, whereas an increase in the number of benzenes along the perpendicular axis does not enhance the γ values. Furthermore, the NLO responses of the reduced form radical anions 1(-), 5(-) and 6(-), which were obtained by a reversible redox process, are discussed. The results show that the γ values of the radical anions are changed by the redox process. For the reduced form radical anion 6(-), the γ value is -1906.71×10(-36)esu, and its absolute value is ∼7.3 times larger than that of its neutral parent. An analysis of the BLA values demonstrates that the γ value is closely related to the conjugation of the aromatic core used in the redox process.
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http://dx.doi.org/10.1016/j.jmgm.2013.01.008DOI Listing
April 2013

Quality evaluation and species differentiation of Rhizoma coptidis by using proton nuclear magnetic resonance spectroscopy.

Anal Chim Acta 2012 Oct 30;747:76-83. Epub 2012 Aug 30.

College of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.

Rhizoma coptidis, a broadly used traditional Chinese medicine, derives from the dried rhizomes of Coptis chinensis Franch, Coptis deltoidea C.Y. Cheng et Hsiao and Coptis teeta Wall. Quantitative determination of protoberberine alkaloids in R. coptidis is critical for controlling its quality. In this study, a rapid, simple and accurate quantitative (1)H NMR (qNMR) method was developed for simultaneous determination of berberine, jatrorrhizine, epiberberine, coptisine, palmatine and columbamine in R. coptidis from the three species. Method validation was performed in terms of selectivity, precision, repeatability, stability, accuracy, robustness and linearity. The average recoveries obtained were in the range of 96.9-102.4% for all the six alkaloids. In addition, the qNMR data were analyzed with analysis of variance (ANOVA), hierarchical clustering analysis (HCA) and principal component analysis (PCA), and the results showed that the contents of the active alkaloids have significant difference among the three species. Compared with the conventional HPLC approach, the proposed qNMR method was demonstrated to be a powerful tool for quantifying the six alkaloids due to its unique advantages of high robustness, rapid analysis time and no need of standard compounds for calibration curves preparation. These findings indicate that this method has potential as a reliable method for quality evaluation of herb medicines, especially for protoberberine alkaloid-containing ones.
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http://dx.doi.org/10.1016/j.aca.2012.08.038DOI Listing
October 2012

[Expression and clinical significance of miR-155 in peripheral blood CD4(+);T cells of patients with allergic asthma].

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2012 May;28(5):540-3

The Laboratory Medicine of Yijishan Hospital, Wannan Medical College, Wuhu, China.

Aim: To investigate the expression and clinical significance of miR-155 in the peripheral blood CD4(+);T cells of patients with allergic asthma.

Methods: The expression of pre-mature BIC transcript and its processed microRNA miR-155 in the CD4(+); T cells from 50 patients with allergic asthma and 20 healthy individuals were measured using real-time PCR. The correlation of miR-155 expression and the disease severity of asthma was analyzed. The ability of miR-155 to influence Th1/Th2 differentiation was examined in vitro.

Results: The expression of pre-mature BIC and miR-155 in the CD4(+); T cells of asthma patients significantly decreased compared to those in normal controls (P<0.01). Furthermore, the expression of BIC in moderate and severe asthma patients was lower than that in normal controls (P<0.05). Among mild, moderate, severe asthma patients, BIC expression showed no statistical significance, but miR-155 expression of severe asthma patients was lower than that in mild asthma patients (P<0.05). The expression level of miR-155 was positively correlated to FEV1/pred(%) (P<0.01). miR-155 over-expression was found to promote CD4(+); T cells to Th1 differentiation, while of miR-155 was found to induce CD4(+); T cells to Th2 differentiation.

Conclusion: The decreased expression level of miR-155 is correlated to asthma disease severity. It is suggested miR-155 plays an important role in development of asthma.
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May 2012

[Suppressive effect of gypenosides on murine leukemia L1210 cell lines].

Zhong Yao Cai 2010 Oct;33(10):1588-92

National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China, Xi'an 710062, China.

Objective: To investigate the suppressive effect of gypenosides (Gyp) on murine leukemia L1210 cells.

Methods: The growth inhabitation of murine leukemia L1210 cell was detected by MT assay. The production of reactive oxygen species and the change of mitochondrial membrane potential were detected by flow cytometry. The change of nuclear and DNA damage of murine leukemia L1210 cells were detected by DAPI staining and single cell gel electrophoresis.

Results: Gyp (100-500 microg/mL) inhibited the growth of murine leukemia L1210 cells. The concentration of Gyp (350 microg/mL) treated murine leukemia L1210 cells at different time points, the mitochondrial membrane potential decrease obviously. L1210 cells were treated with Gyp (350 microg/mL) for 4 h, the highest production of reactive oxygen species was induced. DNA damage were detected after Gyp (350 microg/mL) treated for 4, 12, 24 h. The change of nuclear was treated by Gyp (350 microg/mL) with time-dependent.

Conclusion: Gypenosides has effects on cell viability, induce reactive oxygen species and decreases mitochondrial membrane potential, and can induce morphological changes and DNA damage.
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October 2010
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