Publications by authors named "Meng Zhao"

859 Publications

Assessment of epidermoid cyst with trigeminal neuralgia before neuroendoscopy: A high-resolution MR study based on 3D-FIESTA and MR angiography.

Clin Imaging 2022 Aug 9;91:9-13. Epub 2022 Aug 9.

Department of Radiology, Qilu Hospital of Shandong University, No.107 Wenhuaxi Road, Jinan, China. Electronic address:

Purpose: To assess the value of preoperative 3D-FIESTA and MR angiography (MRA) in endoscopic resection of epidermoid cysts presenting with trigeminal neuralgia (TN).

Methods: 3D-FIESTA and MRA were performed before neuroendoscopy in 32 cases of epidermoid cysts with TN, and the tumors were grouped into types A, B, C, and D according to the relationship between the tumor and adjacent nerves and arteries (Hitoshi Kobata's classification). Evaluation of the neuroendoscopic resectability of different types of tumors, included gross total tumor removal (GTR), subtotal tumor removal (STR), and partial tumor removal (PTR). During the 5-year follow-up, symptoms were assessed based on facial pain relief using the Barrow Neurological Institute (BNI) score.

Results: The coincidence rate between MRI classification of the tumor and the operation was 100%. Type B tumors were the most common (18/32, 56.3%). Type A tumors showed the highest resectability (9/9, 100%), followed by type B tumors (14/18, 77.8%). Moreover, microvascular decompression was performed in all 4 cases of type C and 1 case of type D tumors. During follow-up, 23 patients showed marked improvement in symptoms (15, 8 of BNI I or II), 8 cases showed partial improvement (BNI III), and only 1 case of type C tumor was associated with poor facial pain relief, which recurred 5 years later (BNI IV).

Conclusions: Preoperative high-resolution MR can accurately analyze the relationship between epidermoid cysts and adjacent nerves and arteries. It could act as a powerful tool in the evaluation of tumor resectability and the prognosis of surgery.
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http://dx.doi.org/10.1016/j.clinimag.2022.08.006DOI Listing
August 2022

Mutational Profile and Potential Molecular Therapeutic Targets of Pheochromocytoma.

Front Endocrinol (Lausanne) 2022 28;13:921645. Epub 2022 Jul 28.

Key Laboratory of Endocrinology, Department of Endocrinology, National Health Commission of the People's Republic of China, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, China.

Purpose: Pheochromocytoma/paraganglioma (PCC/PGL; collectively known as PPGL) can be driven by germline and somatic mutations in susceptibility genes. We aimed to investigate the mutation profile and clinical features of pathogenic genes in highly genetically heterogeneous PPGL and to preliminary explore molecular therapeutic targets in PPGL.

Methods: We established a panel of 260 genes, including susceptibility genes of PPGL and other important tumorigenic genes to sequence 107 PPGL tissues.

Results: Overall, 608 genomic mutations were identified in 107 PPGL tissues. Almost 57% of PPGL tissue samples exhibited pathogenic mutations, and the most frequently mutated gene was (15/107, 14%). and were the most commonly mutated genes in germline-mutated PPGL (25/107, 23%) and nongermline-mutated PPGL (36/107, 34%), respectively. In addition, novel pathogenic mutations were detected in sporadic PPGL. PPGL with mutations in the hypoxia pathway had an earlier onset and higher norepinephrine level than those in the kinase pathway. Receptor tyrosine kinase (RTK; 22%, 24/107), mitogen-activated protein kinase (MAPK; 14%, 15/107), and tyrosine kinase (TK; 2%, 2/107) pathways were the most frequently mutated pathways in PPGL.

Conclusion: Our results provided the genetic mutation profile in PPGL tissues. Genetic mutations in PPGL were mainly concentrated in the RTK, TK, and MAPK pathways, suggesting potential molecular therapeutic targets for PPGL.
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http://dx.doi.org/10.3389/fendo.2022.921645DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9368203PMC
August 2022

How do controlled-release fertilizer coated microplastics dynamically affect Cd availability by regulating Fe species and DOC content in soil?

Sci Total Environ 2022 Aug 8:157886. Epub 2022 Aug 8.

Institute of Plan Nutrition, Resources and Environment, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China; Beijing Engineering Technology Research Center for Slow / Controlled-Release Fertilizer, Beijing 100097, China. Electronic address:

Microplastics (MPs) affect the accumulation of heavy metals by regulating the soil environment. However, studies on the dynamic effects of microplastics on the available states of heavy metals in soil are lacking. In particular, how controlled-release fertilizer coated microplastics can synergistically change the avsilable states of heavy metals in soil by affecting soil physical and chemical properties and microbial community structure is still lacking. The dynamic effect of polyurethane (PU) MPs on the effective state of soil cadmium (Cd; DGTCd), at different particle sizes and concentrations, was studied in situ by diffusive gradient in thin-films (DGT) for the first time. The bioavailability, soil chemical properties, and microbial effects of PU MPs on Cd depend on PU particle size and concentration; high-concentration (1 %) PU MPs cause a significant increase in DGT-Cd concentration. The addition of PU MPs decreased soil pH and dissolved oxygen content (DOC), while increasing the absolute zeta value, Fe(II) and Mn(II), in a manner dependent on particle size, concentration, and culture time. Correlation analysis combined with path analysis showed that PU MPs affected the effective state of Cd by changing soil properties, among which Fe(II) content and DOC were important factors controlling the activation of Cd. Meanwhile, changes in soil properties and heavy metal availability correlated significantly with microbial community composition, suggesting that PU MPs may indirectly impact heavy metal activity by affecting microorganisms and functional genes associated with C and Fe cycling. Therefore, when the concentration of PU MPs is higher than 1 %, we should strengthen ecological risk prevention and control of the compound pollution of controlled-release fertilizer coated microplastics and heavy metals in farmland soil.
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http://dx.doi.org/10.1016/j.scitotenv.2022.157886DOI Listing
August 2022

Effect of Iodine Supplementation on Iodine Nutrition and Thyroid Function in Pregnant Women: A Cross-Sectional Study.

Biomed Environ Sci 2022 Jul;35(7):641-647

Center for Endemic Disease Control; Chinese Center for Disease Control and Prevention; Harbin Medical University, Harbin 150081, Heilongjiang, China;Key lab of Etiology and Epidemiology, Education Bureau of Heilongjiang Province & Ministry of Health, Microelement and human health lab of Heilongjiang Province, Center for Endemic Disease Control, Chinese Center for Disease Control and Prevention, Harbin Medical University, Harbin 150081, Heilongjiang, China.

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http://dx.doi.org/10.3967/bes2022.083DOI Listing
July 2022

Prior Guided Transformer for Accurate Radiology Reports Generation.

IEEE J Biomed Health Inform 2022 Aug 8;PP. Epub 2022 Aug 8.

In this paper, we propose a prior guided transformer for accurate radiology reports generation. In the encoder part, a radiograph is firstly represented by a set of patch features, which is obtained through a convolutional neural network and a traditional transformer encoder. Then an Additive Gaussian model is applied to represent the prior knowledge based on unsupervised clustering and sparse attention. In the decoder part, prior embeddings are acquired by probabilistically sampling from the radiograph prior. Then the visual features, language embeddings, and prior embeddings are fused by our proposed Prior Guided Attention to generate accurate radiology reports. Experiment results show that our method achieves better performance than state-of-the-art methods on two public radiology datasets, which proves the effectiveness of our prior guided transformer.
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http://dx.doi.org/10.1109/JBHI.2022.3197162DOI Listing
August 2022

Prognostic Factor Study of Macular Edema Recurrence in Retinal Vein Occlusion after Conbercept Treatment: A Post Hoc Analysis of the FALCON Study.

Comput Math Methods Med 2022 29;2022:3616044. Epub 2022 Jul 29.

Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology and Visual Sciences Key Laboratory, Beijing 100730, China.

Objective: The study was aimed at exploring the potential predictive factors associated with the recurrence of macular edema (ME) secondary to vein occlusion (RVO) after intravitreal antivascular endothelial growth factor (VEGF) loading treatment in the FALCON study.

Methods: This is a post hoc analysis of 30 patients with central RVO and 30 patients with branch RVO. All patients received a monthly administration of intravitreal conbercept during the 3-month loading phase and pro re nata (PRN) treatment during the 6-month follow-up period. Based on the recurrence of ME at the first follow-up visit, patients were classified into the recurrence group or nonrecurrence group. The primary endpoint was to explore the risk factors for recurrence among baseline characteristics, fluorescein angiography (FA) patterns, and optical coherence tomography (OCT).

Results: In general, 38 patients (64.4%) experienced ME recurrence at the first follow-up visit (3 months), regardless of disease type ( = 0.32). Significant improvements in VA were noted in both the nonrecurrence and recurrence groups ( < 0.001), however, without significant between-group differences ( = 0.1). A significant reduction in CRT in both groups ( < 0.001) was identified, and patients without recurrence showed a greater reduction in CRT compared with those with recurrence ( < 0.001). In addition, logistic regression analyses indicated the corrections of ME recurrence with baseline macular volume and the disruption of the outer limiting membrane at the fovea.

Conclusion: This study suggested that OCT parameters, including baseline macular volume and outer limiting membrane disruption, and reduction in CRT after loading therapy were more predictive of ME recurrence than FA patterns or visual changes following conbercept loading therapy.
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http://dx.doi.org/10.1155/2022/3616044DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355755PMC
August 2022

Research progress of placental vascular pathophysiological changes in pregnancy-induced hypertension and gestational diabetes mellitus.

Front Physiol 2022 19;13:954636. Epub 2022 Jul 19.

Institute for Fetology, The First Affiliated Hospital of Soochow University, Suzhou, China.

The placenta is a vital organ for fetal development, providing the fetus with nutrients, oxygen, and other important factors. Placenta is rich in blood vessels. Abnormal placental vascular function and blood circulation may lead to insufficient blood supply to the fetus in the uterus, leading to serious consequences such as pregnancy complications, fetal distress and even stillbirth. Pregnancy-induced hypertension (PIH) and gestational diabetes mellitus (GDM) are common complications of pregnancy. Recent studies report that pregnancy complications are often accompanied by changes in placental vascular structure and function. What are the physiological characteristics of human placental blood vessels? What are the pathological changes in the state of PIH and GDM? What are the relationships between these pathological changes and the occurrence of these pregnancy complications? Answers to these questions not only increase the understanding of placental vascular characteristics, but also provide important information for revealing the pathological mechanism of PIH and GDM. This article will summarize the research on the pathological changes of placental blood vessels in PIH and GDM, hoping to further unravel the physiological and pathological characteristics of placental blood vessels in the state of PIH and GDM, provide information for guiding clinical treatment for PIH and GDM.
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http://dx.doi.org/10.3389/fphys.2022.954636DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9343869PMC
July 2022

Protective effect and mechanism of cannabidiol on myocardial injury in exhaustive exercise training mice.

Chem Biol Interact 2022 Aug 1;365:110079. Epub 2022 Aug 1.

Shenyang Medical College, No. 146, Huanghe North Street, Shenyang, 110034, China. Electronic address:

Cannabinoid diphenol (CBD) is a non-toxic main component extracted from cannabis, which has the effects of anti-inflammatory, anti-apoptosis and anti-oxidative stress. In recent years, exercise-induced myocardial injury has become a research hotspot in the field of sports medicine and sports physiology. Exercise-induced myocardial injury is closely related to oxidative stress, inflammatory response and apoptosis. However, there is no clear evidence of the relationship between CBD and exercise-induced myocardial injury. In this study, by establishing an animal model of exhaustive exercise training in mice, the protective effect of CBD on myocardial injury in mice was elaborated, and the possible molecular mechanism was discussed. After CBD intervention, the arrangement and rupture of myocardial fiber tissue and the degree of inflammatory cell infiltration were reduced, the deposition of collagen fibers in myocardial tissue decreased. CBD can also significantly inhibit cardiac hypertrophy. Meanwhile, the expression of IL-6, IL-10, TNF-α, Bax, Caspase-3, Bcl-2, MDA-5, IRE-1α, NOX-2, SOD-1, Keap1, Nrf2, HO-1, NF-κB and COX-2 was recovered to normal. In addition, after CBD intervention, the protein expression of Keap1 was down-regulated, the translocation of Nrf2 from the cytoplasm to the nucleus was significantly increased, then the transcriptional activity was increased, and the expression of the downstream HO-1 antioxidant protein was increased, indicating that CBD may improve the cardiac function of exhaustive exercise training mice by activating Keap1/Nrf2/HO-1 signaling pathway. Molecular docking results also confirmed that CBD had a good binding effect with Keap1/Nrf2/HO-1 signaling pathway proteins. In conclusion, the protective mechanism of CBD on myocardial injury in exhaustive exercise training mice may be to activate Keap1/Nrf2/HO-1 signaling pathway, and then exert anti-inflammatory, anti-apoptosis and inhibition of oxidative stress.
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http://dx.doi.org/10.1016/j.cbi.2022.110079DOI Listing
August 2022

Direct observation of the Mottness and p-d orbital hybridization in the epitaxial monolayer α-RuCl.

Nanoscale 2022 Aug 2. Epub 2022 Aug 2.

State Key Laboratory of Surface Physics and Department of Physics, Fudan University, Shanghai 200438, China.

α-RuCl, a promising material to accomplish the Kitaev honeycomb model, has attracted enormous interest recently. Mottness and p-d bonds play vital roles in generating Kitaev interactions and underpinning the potential exotic states of quantum magnets, and the van der Waals monolayer is considered to be a better platform to approach a two-dimensional Kitaev model than the bulk. Here, we worked out the growth art of an α-RuCl monolayer on a graphite substrate and studied its electronic structure, particularly the delicate orbital occupations, through scanning tunneling microscopy and spectroscopy. An in-plane lattice expansion of 2.67 ± 0.83% is observed and the pronounced t-p and e-p hybridization are visualized. The Mott nature is unveiled by an ∼0.6 eV full gap at the Fermi level located inside the t-p manifold which is further verified by the density functional theory calculations. The monolayer phase of α-RuCl fulfills the criteria of recent theoretical predictions of tuning the relevant properties in this material and provides a novel platform to explore the Kitaev physics.
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http://dx.doi.org/10.1039/d2nr02827aDOI Listing
August 2022

A Novel Polysaccharide From and Its Protective Effect Against Myocardial Injury.

Front Nutr 2022 14;9:961182. Epub 2022 Jul 14.

School of Basic Medical Sciences, Qingdao University, Qingdao, China.

We isolated and purified a novel polysaccharide from the root of , namely, polysaccharide (CVP) and confirmed its structure and molecular weight. Furthermore, experiment, CVP's protective effect against myocardial ischemia-reperfusion (I/R) injury in mice was evidenced by significantly reducing I/R-induced myocardial infarction (MI) size, decreasing the secretion of heart damage biomarkers, and improving cardiac function. Then, the myocardial anoxia/reoxygenation (A/R) injury model was established to mimic reperfusion injury. Noticeably, ferroptosis was the major death manner for A/R-damaged H9c2 cells. Meanwhile, CVP significantly inhibited ferroptosis by decreasing intracellular Fe level, enhancing GPX4 expression, and suppressing lipid peroxidation to confront A/R injury. In conclusion, CVP, with a clear structure, ameliorated I/R injury by inhibiting ferroptosis.
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http://dx.doi.org/10.3389/fnut.2022.961182DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330552PMC
July 2022

CXCR4 megakaryocytes regulate host-defense immunity against bacterial pathogens.

Elife 2022 Jul 29;11. Epub 2022 Jul 29.

RNA Biomedical Institute, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China.

Megakaryocytes (MKs) continuously produce platelets to support hemostasis and form a niche for hematopoietic stem cell maintenance in the bone marrow. MKs are also involved in inflammatory responses; however, the mechanism remains poorly understood. Using single-cell sequencing, we identified a CXCR4 highly expressed MK subpopulation, which exhibited both MK-specific and immune characteristics. CXCR4 MKs interacted with myeloid cells to promote their migration and stimulate the bacterial phagocytosis of macrophages and neutrophils by producing TNFα and IL-6. CXCR4 MKs were also capable of phagocytosis, processing, and presenting antigens to activate T cells. Furthermore, CXCR4 MKs also egressed circulation and infiltrated into the spleen, liver, and lung upon bacterial infection. Ablation of MKs suppressed the innate immune response and T cell activation to impair the anti-bacterial effects in mice under the challenge. Using hematopoietic stem/progenitor cell lineage-tracing mouse lines, we show that CXCR4 MKs were generated from infection-induced emergency megakaryopoiesis in response to bacterial infection. Overall, we identify the CXCR4 MKs, which regulate host-defense immune response against bacterial infection.
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http://dx.doi.org/10.7554/eLife.78662DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9374440PMC
July 2022

Research Progress of DNA Methylation in Endometrial Cancer.

Biomolecules 2022 07 4;12(7). Epub 2022 Jul 4.

Institute for Fetology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China.

Endometrial cancer (EC)) is one of the most common malignant tumors of the female genital system, with an increasing incidence and mortality, worldwide. Although the therapeutic strategy of EC is still complicated and challenging, further understanding of carcinogenesis from a gene perspective would allow an effort to improve therapeutic precision in this complex malignancy. DNA methylation is the most widely studied epigenetic alteration in human tumors. Aberrant DNA methylation events, resulting in altered gene expression, are features of many tumor types. In this review, we provide an update on evidence about the roles of aberrant DNA methylation within some classical tumor suppressor genes and oncogenes in endometrial carcinogenesis, and report on recent advances in the understanding of the contribution of aberrant DNA methylation to EC, as well as opportunities and challenges of DNA methylation in EC management and prevention.
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http://dx.doi.org/10.3390/biom12070938DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9312849PMC
July 2022

Loss of sphingosine kinase 2 promotes the expansion of hematopoietic stem cells by improving their metabolic fitness.

Blood 2022 Jul 26. Epub 2022 Jul 26.

Sun Yat-sen University, Guangzhou, China.

Hematopoietic stem cells (HSCs) have reduced capacities to properly maintain and replenish the hematopoietic system during myelosuppressive injury or aging. Expanding and rejuvenating HSCs for therapeutic purposes has been a long-sought goal, with limited progress. Here, we show that enzyme sphingosine kinase 2 (Sphk2), which generates the lipid metabolite sphingosine-1-phosphate, is highly expressed in HSCs. The deletion of Sphk2 markedly promotes self-renewal and increases the regenerative potential of HSCs. More importantly, Sphk2 deletion globally preserves the young HSC gene expression pattern, improves the function, and sustains the multilineage potential of HSCs during aging. Mechanistically, Sphk2 interacts with prolyl hydroxylase 2 and the Von Hippel-Lindau protein to facilitate HIF1α ubiquitination in the nucleus independent of the Sphk2 catalytic activity. Deletion of Sphk2 increases hypoxic responses by stabilizing the HIF1α protein to upregulate PDK3, a glycolysis checkpoint protein for HSC quiescence, which subsequently enhances the function of HSCs by improving their metabolic fitness; specifically, it enhances anaerobic glycolysis but suppresses mitochondrial oxidative phosphorylation and generation of reactive oxygen species. Overall, targeting Sphk2 to enhance the metabolic fitness of HSCs is a promising strategy to expand and rejuvenate functional HSCs.
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http://dx.doi.org/10.1182/blood.2022016112DOI Listing
July 2022

Transferrin receptor targeting segment T7 containing peptide gene delivery vectors for efficient transfection of brain tumor cells.

Drug Deliv 2022 Dec;29(1):2375-2385

State Key Laboratory of Toxicology and Medical Countermeasures, Beijing Institute of Pharmacology and Toxicology, Beijing, China.

Successful gene therapy for brain tumors are often limited by two important factors, the existence of blood brain barrier (BBB) and inefficient transfection of brain tumor cells. In this study, we designed a series of peptide-based gene delivery vectors decorated with T7 segment for binding the transferrin (Tf) receptors which were highly expressed on brain tumor cells, and evaluated their ability of gene delivery. The physicochemical properties of peptide vectors or peptide/DNA complexes were studied as well. The transfection efficiency was investigated in normal and glioma cell lines. Among these complexes, PT-02/DNA complexes showed the highest transfection efficiency in glioma cells and low cytotoxicity in normal cell lines, and it could transport DNA across the BBB model . Furthermore, PT-02/DNA could deliver pIRES2-EGFP into the brain site of zebrafish . The designed peptide vectors offered a promising way for glioma gene therapy.
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http://dx.doi.org/10.1080/10717544.2022.2102696DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9310815PMC
December 2022

The Effects of Exercise Interventions on Mental Health in Chinese Older Adults.

J Environ Public Health 2022 30;2022:7265718. Epub 2022 Jun 30.

Shanwei Vocational and Technical College, Shanwei 516600, China.

Objectives: The aim of this meta-analysis was to comprehensively evaluate the effects of exercise interventions on mental health in Chinese older adults, according to the effects of intervention methods, intervention duration, intervention frequency, intervention time, measurement tools, and subject types.

Methods: We searched CNKI, Wanfang and Technology Journal Database for randomized control trials, 15 original studies were extracted, reporting on 1509 Chinese older adults (Age: 55-79 years old). We used CMA2.0 software. After heterogeneity test, suitable random effect model was selected.

Results: Physical exercise had a positive effect on mental health in Chinese older adults, combined effect [SMD = -1.25, 95% CI (-1.66, -0.84), ≤ 0.001], anxiety combined effect size [SMD = -1.45, 95% CI (-2.08, -0.83), ≤ 0.001], depression combined effect size [SMD = -1.02, 95% CI (-1.56, -0.48), ≤ 0.001]. Significant differences in the intervention types, intervention duration, intervention frequency, intervention time, and measurement tools were found.

Conclusion: Physical exercise had positive intervention effect on the mental health of the Chinese older adults, which was applicable to all older adults. Exercise rehabilitation was the more effective, and the best intervention effect on the older hypertension. Due to the high heterogeneity of this study and the limited number of included literature, more high-quality needed to be included in the subsequent studies.
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http://dx.doi.org/10.1155/2022/7265718DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9277469PMC
July 2022

Differential Expression Profiles of mRNA and Noncoding RNA and Analysis of Competitive Endogenous RNA Regulatory Networks in Nonalcoholic Steatohepatitis.

Gastroenterol Res Pract 2022 7;2022:3200932. Epub 2022 Jul 7.

Department of Endocrinology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong 250021, China.

Nonalcoholic steatohepatitis (NASH) is a liver disease caused by multiple factors, and there is no approved pharmacotherapy. The pathogenesis of NASH remains underexplored. In this study, differentially expressed circular RNAs (circRNAs) were obtained by analyzing NASH-related circRNA datasets, and then, corresponding target microRNAs (miRNAs) and messenger RNAs (mRNAs) were predicted to construct a circRNA-miRNA-mRNA regulatory network. On this basis, a total of 38 circRNAs, 7 miRNAs, and 10 mRNAs were screened out. The present study reveals novel circRNA biomarkers of NASH and reports a potential competing endogenous RNA (ceRNA) regulatory network that might provide insights for further investigation into the underlying pathogenesis of NASH.
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http://dx.doi.org/10.1155/2022/3200932DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9282983PMC
July 2022

Simulated adsorption of iodine by an amino-metal-organic framework modified with covalent bonds.

Environ Sci Pollut Res Int 2022 Jul 16. Epub 2022 Jul 16.

College of Chemistry and Chemical Engineering, Qinghai Normal University, No. 38, Wusi West Road, Chengxi District, Xining City, 810008, Qinghai, China.

Radioactive iodine in nuclear waste is increasingly harmful to the human body and the environment because of its strong radioactivity, high fluidity, easy solubility in water, and long half-life. It is very important to find clean and economical materials to recover and fix radioactive iodine. In this paper, the amino-metal-organic framework was covalently modified to obtain composite materials to improve the recycling of iodine in the environment. These adsorbents are used to adsorb iodine in water, showing outstanding adsorption performance. The adsorption data are in good agreement with the Langmuir isothermal adsorption model and pseudo-second-order kinetic model, indicating that the adsorption process is mainly monolayer adsorption and chemical adsorption. The two materials showed selective adsorption capacity for iodine in the solution containing multiple competing ions. The adsorption capacity of the covalently modified composite increased from 949.52 to 2157.44 mg/g. Compared with the amino-metal-organic framework, the modified composite contains more electron-rich groups as active sites, and forms charge transfer compounds with iodine to realize chemical adsorption. Through the simulated adsorption of ultra-high-pressure micro-jet, the material has certain working ability under high pressure, which provides a theoretical basis for the future recovery and utilization of iodine under high pressure.
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http://dx.doi.org/10.1007/s11356-022-21971-8DOI Listing
July 2022

Identification of a novel heterozygous missense TP63 variant in a Chinese pedigree with split-hand/foot malformation.

BMC Med Genomics 2022 07 13;15(1):157. Epub 2022 Jul 13.

Department of Obstetrics and Gynecology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, Jiangsu, China.

Background: Tumor protein p63 is an important transcription factor regulating epithelial morphogenesis. Variants associated with the TP63 gene are known to cause multiple disorders. In this study, we determined the genetic cause of split-hand/foot malformation in a Chinese pedigree.

Methods: For this study, we have recruited a Chinese family and collected samples from affected and normal individuals of the family (three affected and two normal). Whole exome sequencing was performed to detect the underlying genetic defect in this family. The potential variant was validated using the Sanger sequencing approach.

Results: Using whole-exome and Sanger sequencing, we identified a novel heterozygous pathogenic missense variant in TP63 (NM_003722.5: c.921G > T; p.Met307Ile). This variant resulted in the substitution of methionine with isoleucine. Structural analysis suggested a resulting change in the structure of a key functional domain of the p63 protein.

Conclusion: This novel missense variant expands the TP63 variant spectrum and provides a basis for genetic counseling and prenatal diagnosis of families with split-hand/foot malformation or other TP63-related diseases.
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http://dx.doi.org/10.1186/s12920-022-01311-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9281006PMC
July 2022

Risk Factors for Short Stature in Children Born Small for Gestational Age at Full-Term.

Front Pediatr 2022 22;10:833606. Epub 2022 Jun 22.

Department of Pediatrics, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Objective: This study aims to identify the risk factors associated with short stature in children born small for gestational age (SGA) at full-term.

Methods: This was a retrospective study. The subjects were full-term SGA infants who were followed up until the age of 2 years. The risk factors for short stature were identified with univariate and multivariate analyses.

Results: Of 456 full-term SGA children enrolled in this study, 28 cases had short stature at 2 years of age. A significant decrease in placental perfusion was found in the short children group with intravoxel incoherent motion (IVIM) technology, which was an advanced bi-exponential diffusion-weighted imaging (DWI) model of magnetic resonance imaging (MRI) ( = 0.012). Compared to non-short children born SGA at full-term, the short children group underwent an incomplete catch-up growth. Mothers who suffered from systemic lupus erythematosus were more likely to have a short child born SGA ( = 0.023). The morbidity of giant placental chorioangioma was higher in the short children group. The pulsatility index (PI), resistivity index (RI), and systolic-diastolic (S/D) ratio of umbilical artery were higher in the short children group than in the non-short control group ( = 0.042, 0.041, and 0.043). Multivariate analysis demonstrated that decrease of perfusion fraction ( ) in IVIM of placental MRI, chromosomal abnormalities, short parental height, and absence of catch-up growth were associated with a higher risk of short stature in children born SGA at full-term.

Conclusion: Risk factors for short stature in full-term SGA children at 2 years of age included a decrease of perfusion fraction in IVIM of placental MRI, chromosomal abnormalities, and short parental height.
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http://dx.doi.org/10.3389/fped.2022.833606DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9256917PMC
June 2022

Association of Dietary Vitamin K Intake With Cognition in the Elderly.

Front Nutr 2022 23;9:900887. Epub 2022 Jun 23.

Department of Epidemiology and Health Statistics, School of Public Health, Qingdao University, Qingdao, China.

Several previous studies discussed the association between vitamin K (VK) status and cognition. But the association between dietary VK consumption and cognitive performance in the elderly was not well understood. Therefore, we investigated the correlation between dietary VK intake and the cognition of the elderly. Our research used the data of the National Health and Nutrition Examination Survey (NHANES) from 2011 to 2014. The dietary intake of VK was assessed by two 24-h dietary recalls. The cognitive function was measured in the survey of NHANES, including the Consortium to Establish a Registry for Alzheimer's disease Word Learning subtest (CERAD W-L), Animal Fluency Test (AFT), and Digit Symbol Substitution Test (DSST). Logistic regression and restricted cubic spline models were applied to assess the relationship between dietary VK intake and cognition. Compared with the lowest dietary VK intake group, the multivariate-adjusted odds ratio (OR) [95% confidence interval (95% CI)] of low CERAD W-L score for the highest intake group was 0.39 (0.26-0.60), the multivariate-adjusted OR (95% CI) of low AFT score was 0.59 (0.38-0.92), and the multivariate-adjusted OR (95% CI) of low DSST score was 0.44 (0.29-0.65), respectively. There was an L-shaped dose-response relationship between dietary VK intake and low CERAD W-L score. There was a linear dose-response relationship between dietary VK intake and low AFT score, and there was also a linear dose-response relationship for the low DSST score. In addition, we also found a negative association between VK from vegetables and the risk of low CERAD W-L scores. Dietary VK intake and VK intake from vegetables were inversely related to the risk of low cognitive performance of the elderly.
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http://dx.doi.org/10.3389/fnut.2022.900887DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9260313PMC
June 2022

Adsorption of Different Pollutants by Using Microplastic with Different Influencing Factors and Mechanisms in Wastewater: A Review.

Nanomaterials (Basel) 2022 Jun 30;12(13). Epub 2022 Jun 30.

School of Environmental Science and Engineering, Guangzhou University, Guangzhou 510006, China.

The studies on microplastics are significant in the world. According to the literature, microplastics have greatly specific surface areas, indicating high adsorption capacities for highly toxic pollutants in aquatic and soil environments, and these could be used as adsorbents. The influencing factors of microplastic adsorption, classification of microplastics, and adsorption mechanisms using microplastics for adsorbing organic, inorganic, and mixed pollutants are summarized in the paper. Furthermore, the influence of pH, temperature, functional groups, aging, and other factors related to the adsorption performances of plastics are discussed in detail. We found that microplastics have greater advantages in efficient adsorption performance and cost-effectiveness. In this paper, the adsorptions of pollutants by microplastics and their performance is proposed, which provides significant guidance for future research in this field.
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http://dx.doi.org/10.3390/nano12132256DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9268391PMC
June 2022

Amino acid catabolism regulates hematopoietic stem cell proteostasis via a GCN2-eIF2α axis.

Cell Stem Cell 2022 Jul;29(7):1119-1134.e7

RNA Biomedical Institute, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510000, China; Key Laboratory of Stem Cells and Tissue Engineering (Ministry of Education), Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, Guangdong 510000, China. Electronic address:

Hematopoietic stem cells (HSCs) adapt their metabolism to maintenance and proliferation; however, the mechanism remains incompletely understood. Here, we demonstrated that homeostatic HSCs exhibited high amino acid (AA) catabolism to reduce cellular AA levels, which activated the GCN2-eIF2α axis, a protein synthesis inhibitory checkpoint to restrain protein synthesis for maintenance. Furthermore, upon proliferation conditions, HSCs enhanced mitochondrial oxidative phosphorylation (OXPHOS) for higher energy production but decreased AA catabolism to accumulate cellular AAs, which inactivated the GCN2-eIF2α axis to increase protein synthesis and coupled with proteotoxic stress. Importantly, GCN2 deletion impaired HSC function in repopulation and regeneration. Mechanistically, GCN2 maintained proteostasis and inhibited Src-mediated AKT activation to repress mitochondrial OXPHOS in HSCs. Moreover, the glycolytic metabolite, NAD precursor nicotinamide riboside (NR), accelerated AA catabolism to activate GCN2 and sustain the long-term function of HSCs. Overall, our study uncovered direct links between metabolic alterations and translation control in HSCs during homeostasis and proliferation.
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http://dx.doi.org/10.1016/j.stem.2022.06.004DOI Listing
July 2022

Peritoneal M2 macrophage-derived extracellular vesicles as natural multitarget nanotherapeutics to attenuate cytokine storms after severe infections.

J Control Release 2022 Jul 6;349:118-132. Epub 2022 Jul 6.

NHC Key Laboratory of Transplant Engineering and Immunology, Regenerative Medicine Research Center, Frontiers Science Center for Disease-related Molecular Network, West China Hospital of Sichuan University, Chengdu, China. Electronic address:

Cytokine storms are a primary cause of multiple organ damage and death after severe infections, such as SARS-CoV-2. However, current single cytokine-targeted strategies display limited therapeutic efficacy. Here, we report that peritoneal M2 macrophage-derived extracellular vesicles (M2-EVs) are multitarget nanotherapeutics that can be used to resolve cytokine storms. In detail, primary peritoneal M2 macrophages exhibited superior anti-inflammatory potential than immobilized cell lines. Systemically administered M2-EVs entered major organs and were taken up by phagocytes (e.g., macrophages). M2-EV treatment effectively reduced excessive cytokine (e.g., TNF-α and IL-6) release in vitro and in vivo, thereby attenuating oxidative stress and multiple organ (lung, liver, spleen and kidney) damage in endotoxin-induced cytokine storms. Moreover, M2-EVs simultaneously inhibited multiple key proinflammatory pathways (e.g., NF-κB, JAK-STAT and p38 MAPK) by regulating complex miRNA-gene and gene-gene networks, and this effect was collectively mediated by many functional cargos (miRNAs and proteins) in EVs. In addition to the direct anti-inflammatory role, human peritoneal M2-EVs expressed angiotensin-converting enzyme 2 (ACE2), a receptor of SARS-CoV-2 spike protein, and thus could serve as nanodecoys to prevent SARS-CoV-2 pseudovirus infection in vitro. As cell-derived nanomaterials, the therapeutic index of M2-EVs can be further improved by genetic/chemical modification or loading with specific drugs. This study highlights that peritoneal M2-EVs are promising multifunctional nanotherapeutics to attenuate infectious disease-related cytokine storms.
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http://dx.doi.org/10.1016/j.jconrel.2022.06.063DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9257240PMC
July 2022

Antenatal Dexamethasone Exposure Impairs Vascular Contractile Functions via Upregulating IP3 Receptor 1 and Cav1.2 in Adult Male Offspring.

Hypertension 2022 Sep 28;79(9):1997-2007. Epub 2022 Jun 28.

Institute for Fetology, The First Affiliated Hospital of Soochow University, Suzhou, China (T.X., B.J., L.L., J.L., M.Z., M.S., Z.X., Q.G.).

Background: Administration of antenatal glucocorticoids remains common practice for treating preterm delivery. Antenatal glucocorticoid exposure increased the risk of developing vascular diseases in later life, but the precise mechanisms remain unclear. This study aimed to explore the effects and mechanisms of antenatal exposure to clinically relevant doses of dexamethasone (synthetic glucocorticoids) on vascular functions in adult male offspring.

Methods: Pregnant Sprague-Dawley rats received dexamethasone or vehicle during the last week of pregnancy. Male offspring were killed at gestational day 21 (Fetus) or postnatal day 120 (adult offspring). Mesenteric arteries were collected for vascular function, electrophysiology, target gene expression, and promotor methylation studies.

Results: Antenatal dexamethasone exposure increased phenylephrine-mediated vascular contractility in offspring, which was resulted by the activated inositol 1,4,5-trisphosphate (IP3) receptor and L-type Ca channels. Specifically, increases of IP3R1 (IP3 receptor 1) and Cav1.2 (L-type Ca channels subunit alpha1 C) were responsible for an activated IP3-Ca pathway in the vasculature, and eventually predisposed the antenatal dexamethasone offspring to vascular hypercontractility. In addition, IP3R1 and Cav1.2 was upregulated through transcriptional mechanism; the overall changes in promotor histone modifications were consistent with the corresponding changes in transcriptional levels of the 2 genes, suggesting that antenatal dexamethasone exposure activated the transcription of IP3R1 and Cav1.2 via altering promotor histone modifications.

Conclusions: Taken together, this study demonstrated that antenatal dexamethasone exposure resulted in vascular adverse outcomes in male offspring that is linked to the increases of IP3R1 and Cav1.2 mediated by epigenetic modifications in the vasculature.
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http://dx.doi.org/10.1161/HYPERTENSIONAHA.122.19040DOI Listing
September 2022

Synthetic-to-real: instance segmentation of clinical cluster cells with unlabeled synthetic training.

Bioinformatics 2022 06;38(Suppl 1):i53-i59

Engineering Research Center of Learning-Based Intelligent System (Ministry of Education), The Key Laboratory of Computer Vision and System (Ministry of Education), and the School of Computer Science and Engineering, Tianjin University of Technology, Tianjin 300384, China.

Motivation: The presence of tumor cell clusters in pleural effusion may be a signal of cancer metastasis. The instance segmentation of single cell from cell clusters plays a pivotal role in cluster cell analysis. However, current cell segmentation methods perform poorly for cluster cells due to the overlapping/touching characters of clusters, multiple instance properties of cells, and the poor generalization ability of the models.

Results: In this article, we propose a contour constraint instance segmentation framework (CC framework) for cluster cells based on a cluster cell combination enhancement module. The framework can accurately locate each instance from cluster cells and realize high-precision contour segmentation under a few samples. Specifically, we propose the contour attention constraint module to alleviate over- and under-segmentation among individual cell-instance boundaries. In addition, to evaluate the framework, we construct a pleural effusion cluster cell dataset including 197 high-quality samples. The quantitative results show that the numeric result of APmask is > 90%, a more than 10% increase compared with state-of-the-art semantic segmentation algorithms. From the qualitative results, we can observe that our method rarely has segmentation errors.
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http://dx.doi.org/10.1093/bioinformatics/btac219DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9235483PMC
June 2022

Correlation Between Ictal Signs and Anatomical Subgroups in Temporal Lobe Seizures: A Stereoelectroencephalography Study.

Front Neurol 2022 10;13:917079. Epub 2022 Jun 10.

Department of Neurosurgery, Center of Epilepsy, Beijing Institute for Brain Disorders, Beijing Key Laboratory of Epilepsy Research, Sanbo Brain Hospital, Capital Medical University, Beijing, China.

Objective: Ictal semiology is a fundamental part of the presurgical evaluation of patients with temporal lobe epilepsy. We aimed to identify different anatomical and semiologic subgroups in temporal lobe seizures, and investigate the correlation between them.

Methods: We enrolled 93 patients for whom stereoelectroencephalography exploration indicated that the seizure-onset zone was within the temporal lobe. Ictal signs and concomitant stereoelectroencephalography changes were carefully reviewed and quantified, and then cluster analysis and the Kendall correlation test were used to associate ictal signs with the temporal structures of patients.

Results: Clustering analysis identified two main groups of temporal structures. Group 1 consisted of the medial temporal lobe structures and the temporal pole, which were divided into two subgroups. Group 1A included the hippocampal head, hippocampal body, and amygdala, and this subgroup correlated significantly with oroalimentary automatisms, feeling of fear, and epigastric auras. Group 1B included the hippocampal tail, temporal pole, and parahippocampal gyrus, and this subgroup correlated significantly with manual and oroalimentary automatisms. Group 2 consisted of the cortical structures of the temporal lobe and was also divided into two subgroups. Group 2A included the superior and middle temporal gyrus, correlated significantly with bilateral rictus/facial contraction, generalized tonic-clonic seizure, and manual automatisms. Group 2B included Heschl's gyrus, the inferior temporal gyrus, and the fusiform gyrus, and this subgroup correlated significantly with auditory auras, focal hypokinetics, unilateral upper and lower limbs tonic posture/clonic signs, head/eye deviation, unilateral versive signs, and generalized tonic-clonic seizure.

Significance: The temporal structures can be categorized according to the level at which each structure participates in seizures, and different anatomical subgroups can be correlated with different ictal signs. Identifying specific semiologic features can help us localize the epileptogenic zone and thus develop stereoelectroencephalography electrode implantation and surgical resection protocols for patients with temporal lobe epilepsy.
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http://dx.doi.org/10.3389/fneur.2022.917079DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9226566PMC
June 2022

Exogenous Hemin alleviates cadmium stress in maize by enhancing sucrose and nitrogen metabolism and regulating endogenous hormones.

Int J Phytoremediation 2022 Jun 22:1-13. Epub 2022 Jun 22.

College of Agriculture, Northeast Agricultural University, Harbin, China.

Cadmium (Cd) stress restricts maize growth and productivity severely. We aimed to investigate the effects of Hemin on the metabolism of sucrose and nitrogen and endogenous hormones in maize under cadmium stress. Maize varieties 'Tiannong 9' (cadmium tolerant) and 'Fenghe 6' (cadmium sensitive) were grown in nutrient solutions to study the effects of Hemin on maize physiological and ecological mechanisms under cadmium stress. The results showed that Hemin mediated the increase of sucrose content and the activities of key enzymes sucrose phosphate synthase (SPS) and sucrose synthase (SS) in maize leaves under cadmium stress. Soluble acid invertase (SAInv) and basic/neutral invertase (A/N-Inv) enzyme activities in leaves were decreased significantly, and sucrose accumulation in leaves was increased. Hemin also mediated the increase of NO content in leaves, the decrease of NH content and the increase of nitrate reductase (NR), glutamine synthetase (GS), glutamate synthase activity (GOGAT) and glutamate dehydrogenase (GDH) enzyme activities under cadmium stress. The contents of IAA, ZR, and GA in leaves and roots increased, ABA, MeJA, and SA decreased, and IAA/ABA, ZR/ABA, and GA/ABA increased under cadmium stress. Our study showed Hemin can alleviate cadmium stress in maize by enhancing sucrose and nitrogen metabolism and regulating endogenous hormones.
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http://dx.doi.org/10.1080/15226514.2022.2086212DOI Listing
June 2022

FTO-mediated m A modification of SOCS1 mRNA promotes the progression of diabetic kidney disease.

Clin Transl Med 2022 Jun;12(6):e942

Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou, Jiangsu, China.

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http://dx.doi.org/10.1002/ctm2.942DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9217105PMC
June 2022

Plasma miR-193b-3p Is Elevated in Type 2 Diabetes and Could Impair Glucose Metabolism.

Front Endocrinol (Lausanne) 2022 27;13:814347. Epub 2022 May 27.

Department of Occupational and Environmental Health and State Key Laboratory of Environmental Health for Incubating, School of Public Health, Huazhong University of Science and Technology, Wuhan, China.

Objective: To explore differentially expressed miRNAs in type 2 diabetes and their potential cellular functions.

Methods: We screened plasma miRNAs by miRNA array analysis and validated them by TaqMan real-time PCR in 113 newly diagnosed, untreated type 2 diabetes cases and 113 healthy controls. Low-abundance plasma proteins encoded by miR-193b-3p target genes were explored in this study population. We further investigated the potential cellular functions of the differentially expressed miRNAs in HepG2 cells.

Results: miR-193b-3p was differentially expressed in type 2 diabetes cases compared to healthy controls (fold change = 2.01, = 0.006). Plasma levels of triosephosphate isomerase (TPI1, a protein involved in the glycolytic pathway) decreased in type 2 diabetes cases (fold change = 1.37, = 0.002). The effect of miR-193b-3p on TPI1 was verified by transfection of miR-193b-3p into HepG2 cells. miR-193b-3p inhibited the expression of YWHAZ/14-3-3ζ in the PI3K-AKT pathway, subsequently altering the expression of FOXO1 and PCK1. After transfection, cells were incubated in glucose-free medium for another 4 h. Glucose levels in medium from cells with elevated miR-193b-3p levels were significantly higher than those in medium from negative control cells ( = 0.016). In addition, elevated miR-193b-3p reduced glucose uptake by inhibiting insulin receptor (IR) and GLUT2 expression.

Conclusion: Plasma miR-193b-3p levels increased in type 2 diabetes cases, and TPI1 levels decreased in both plasma and HepG2 cells with increased miR-193b-3p levels, while extracellular lactate levels did not significantly changed. Moreover, miR-193b-3p may affect glucose metabolism by directly targeting YWHAZ/14-3-3ζ and upregulating the transcription factor FOXO1 downstream of the PI3K-AKT pathway.
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http://dx.doi.org/10.3389/fendo.2022.814347DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9197112PMC
May 2022

Unveiling the Role of DNA Methylation in Vascular CACNA1C Tissue-Specific Expression.

Front Cardiovasc Med 2022 31;9:872977. Epub 2022 May 31.

Institute for Fetology, First Hospital of Soochow University, Suzhou, China.

Objective: Calcium voltage-gated channel subunit alpha1 C (CACNA1C) plays a critical role in many vascular physiological and pathological processes. Determining its tissue-specific expression pattern and clarifying the underlying molecular mechanisms are necessary and meaningful.

Methods: We selected several representative vessels from normal male Sprague-Dawley rats. Vessel tissue or primary vascular smooth muscle cells were isolated for vascular function, electrophysiology, gene expression and promoter methylation studies.

Results: We found CACNA1C had tissue-specific expressions in vessels. The specific manifestations were as follows: CACNA1C expression was highest in thoracic aorta, second lowest in middle cerebral and pulmonary artery, and lowest in mesenteric artery. Excitingly, an opposing trend was observed between CACNA1C expression and its promoter methylation.

Conclusions: This study was the first report to indicate that DNA methylation could be involved in regulating CACNA1C tissue-specific expressions and vasoconstriction function in vascular system. This study not only provided more information for further understanding the physiological characteristics of vascular CACNA1C expressions, also strengthened the idea that DNA methylation plays important roles in regulating vascular smooth muscle cells function and the consequent occurrence of vascular diseases.
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http://dx.doi.org/10.3389/fcvm.2022.872977DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9197502PMC
May 2022
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