Publications by authors named "Meng Zhang"

2,275 Publications

  • Page 1 of 1

Evolution of Photoluminescent CdS Magic-Size Clusters Assisted by Adding Small Molecules with Carboxylic Group.

ACS Omega 2021 Jun 28;6(22):14458-14466. Epub 2021 May 28.

Engineering Research Center in Biomaterials, Sichuan University, Chengdu, Sichuan 610065, P. R. China.

We report our investigation on the formation of photoluminescent CdS magic-size clusters (MSCs), which exhibit absorption peaking at 373 nm, along with sharp band edge emission at ∼385 nm. Denoted as MSC-373, the MSCs were synthesized from the reaction of cadmium oleate (Cd(OA)) and S powder in 1-octadecene at room temperature, together with the addition of acetic acid (HOAc) or acetate salts (M(OAc), M = Zn and Mn) during the prenucleation stage (120 °C). The morphology of as-synthesized MSC-373 was dot-like, which could be altered to flake-like morphology after purification. We found the formation of MSC-373 was related to the ligand exchange, resulting from the addition of small molecules with carboxylic group. The addition of HOAc not only promotes the formation of CdS MSC-373 but suppresses the formation of MSC-311 and nucleation and growth of quantum dots (QDs). When the amount of HOAc addition was increased, another photoluminescent CdS MSCs, namely, MSC-406, evolved. This study provides an overall understanding of the CdS MSC-373 and introduces a new approach to synthesize photoluminescent CdS MSCs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsomega.1c01362DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8190887PMC
June 2021

An iron-air fuel cell system towards concurrent phosphorus removal and resource recovery in the form of vivianite and energy generation in wastewater treatment: A sustainable technology regarding phosphorus.

Sci Total Environ 2021 Jun 6;791:148213. Epub 2021 Jun 6.

Shaanxi Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, PR China. Electronic address:

Phosphorous (P) recovery from industrial wastewaters solves both P deficiency and P pollution problems. A sequencing batch iron-air fuel cell was set up to recover P from synthetic wastewater containing 0.6 g-P/L NaHPO. In the cell, ferrous iron goes into the liquor from iron-anode to precipitate soluble P and form vivianite. Electrons travel from iron-anode to air-cathode through external circuit thus to generate energy. During 3 months' continuous operation, the P removal efficiency stably achieved at around 97.6%, and the average output voltage of cell was 404 mV. After long time operation, performance degradation of iron-air fuel cell was observed due to the electrode passivation caused by the accumulation of P precipitate on the iron-anode surface. The precipitate layer on the iron-anode impeded, but it did not block the mass transfer of ferrous iron to the anode liquor. The cell still worked with 25% decrease of output voltage, 86% decrease of current density, 87% decrease of power density and 9 times increase of internal resistance. Further analyses by XRD, FITR and Mössbauer illustrated that vivianite was the main component in both precipitates on the iron-anode surface and at the bottom of anode chamber with respective content of 66% and 30%. Vivianite on the iron-anode surface was a preferable choice due to higher content for P recovery. The iron-air fuel cell system could be a feasible option for achieving the multiple goals of P pollution control, resource recovery as vivianite, and energy generation, thereby contributing to the sustainable development of wastewater treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2021.148213DOI Listing
June 2021

The Prognostic Role of Peritumoral Edema in Patients with Newly Diagnosed Glioblastoma: A Retrospective Analysis.

J Clin Neurosci 2021 Jul 15;89:249-257. Epub 2021 May 15.

The Department of Neurosurgery, The First Medical Centre, Chinese PLA General Hospital, Fuxing Road 28, Haidian District, Beijing 100853, China. Electronic address:

Objective: Previous studies on glioblastomas (GBMs) have not reached a consensus on peritumoral edema (PTE)'s influence on survival. This study evaluated the PTE index's prognostic role in newly diagnosed GBMs using a well-designed method.

Methods: Selected patients were reviewed after a rigorous screening process. Their general information was obtained from electronic medical records. The imaging metrics (MTD, TTM, TTE) representing tumor diameter, laterality, and PTE extent were obtained by manual measurement in Syngo FastView software. The PTE index was a ratio of TTE to MTD. Multiple variables were evaluated using analysis of variance and Cox regression model.

Results: Of 143 patients, 62 were included in this study. MGMT promoter methylation and tumor laterality were both independent prognostic factors (p = 0.020, 0.042; HR = 0.272, 2.630). The lateral tumors' index was higher than that of the medial tumors (57.7% vs. 42.6%, p = 0.027). Low-index tumors were located in relatively medial positions compared with high-index tumors (TTM, 4.9 vs. 12.8, p = 0.032). This finding indicated that the PTE index tended to increase with tumor laterality. Moreover, the patients with low-index tumors had a significant survival disadvantage in the univariate analysis but not in the multivariate analysis (p = 0.023, 0.220). However, further analysis found that the combination of tumor laterality and PTE statistically stratified the survival outcome. The patients with lateral high-index tumors survived significantly longer (p = 0.022, HR = 1.927).

Conclusions: In contrast with the previous studies, this study recommends combining PTE and tumor laterality for survival stratification in newly diagnosed GBMs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jocn.2021.04.042DOI Listing
July 2021

An Efficient Sharing Grouped Convolution via Bayesian Learning.

IEEE Trans Neural Netw Learn Syst 2021 Jun 10;PP. Epub 2021 Jun 10.

Compared with traditional convolutions, grouped convolutional neural networks are promising for both model performance and network parameters. However, existing models with the grouped convolution still have parameter redundancy. In this article, concerning the grouped convolution, we propose a sharing grouped convolution structure to reduce parameters. To efficiently eliminate parameter redundancy and improve model performance, we propose a Bayesian sharing framework to transfer the vanilla grouped convolution to be the sharing structure. Intragroup correlation and intergroup importance are introduced into the prior of the parameters. We handle the Maximum Type II likelihood estimation problem of the intragroup correlation and intergroup importance by a group LASSO-type algorithm. The prior mean of the sharing kernels is iteratively updated. Extensive experiments are conducted to demonstrate that on different grouped convolutional neural networks, the proposed sharing grouped convolution structure with the Bayesian sharing framework can reduce parameters and improve prediction accuracy. The proposed sharing framework can reduce parameters up to 64.17%. For ResNeXt-50 with the sharing grouped convolution on ImageNet dataset, network parameters can be reduced by 96.875% in all grouped convolutional layers, and accuracies are improved to 78.86% and 94.54% for top-1 and top-5, respectively.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1109/TNNLS.2021.3084900DOI Listing
June 2021

Robust Molecular Dipole-Enabled Defect Passivation and Control of Energy Level Alignment for High-Efficiency Perovskite Solar Cells.

Angew Chem Int Ed Engl 2021 Jun 9. Epub 2021 Jun 9.

Georgia Institute of Technology, School of Materials Science and Engineering, 771 Ferst Dr., NW, 3100K, Molecular Science & Engineering Bldg., 30332, Atlanta, UNITED STATES.

The ability to passivate defects and modulate the interface energy-level alignment (IEA) is key to boost the performance of perovskite solar cells (PSCs). Herein, we report a robust route that simultaneously allows defect passivation and reduced energy difference between perovskite and hole transport layer (HTL) via the judicious placement of polar chlorine-terminated silane molecules at the interface. Density functional theory (DFT) points to effective passivation of the halide vacancies on perovskite surface by the silane chlorine atoms. An integrated experimental and DFT study demonstrates that the dipole layer formed by the silane molecules decreases the perovskite work function, imparting an Ohmic character to the perovskite/HTL contact. The corresponding PSCs manifest a nearly 20% increase in power conversion efficiency over pristine devices and a markedly enhanced device stability. As such, the use of polar molecules to passivate defects and tailor the IEA in PSCs presents a promising platform to advance the performance of PSCs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/anie.202105512DOI Listing
June 2021

Alzheimer's Disease and microRNA-132: A Widespread Pathological Factor and Potential Therapeutic Target.

Front Neurosci 2021 24;15:687973. Epub 2021 May 24.

Department of Otolaryngology Head and Neck Surgery, Shengjing Hospital of China Medical University, Shenyang, China.

Alzheimer's disease (AD) is a common neurodegenerative disease in the elderly and is the most common type of dementia. AD is mostly gradual onset, and involves slow, progressive mental decline, accompanied by personality changes; the incidence of AD gradually increases with age. The etiology of AD is unknown, although it is currently believed to be related to abnormal deposition of amyloid β-protein (Aβ) in the brain, hyperphosphorylation of microtubule-associated protein tau, and the release of various cytokines, complements, activators and chemokines by cells. MicroRNAs (miRNAs) are a class of highly conserved non-coding RNAs that regulate gene expression at the post-transcriptional level, and manipulate the functions of intracellular proteins and physiological processes. Emerging studies have shown that miRNA plays an important role in regulating AD-related genes. MiR-132 is known as "NeurimmiR" due to its involvement in numerous neurophysiological and pathological processes. Accumulating pre-clinical results suggest that miR-132 may be involved in the progression of Aβ and tau pathology. Moreover, clinical studies have indicated that decreased circulating miR-132 levels could be used a potential diagnostic biomarker in AD. Here, we review the pathogenic role of miR-132 activity in AD, and the potential of targeting miR-132 for developing future therapeutic strategies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fnins.2021.687973DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8180577PMC
May 2021

The complete chloroplast genome sequence of cv. Wamin.

Mitochondrial DNA B Resour 2021 May 21;6(6):1704-1705. Epub 2021 May 21.

College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.

cv. Wamin is an attractive ornamental bamboo species of southern China. It has large swollen internodes and weeping culms, and it has considerable economic importance. In the present study, we sequenced the complete chloroplast genome of cv. Wamin and reported it for the first time. The genome was 139,528 bp in total length, including a large single-copy (LSC) region of 83,038 bp, a small single-copy (SSC) region of 12,893 bp, and a pair of invert repeats (IR) regions of 21,799 bp. Plastid genome contained 138 genes, 82 protein-coding genes, 38 tRNA genes, and 8 rRNA genes. The overall GC content of the genome was 38.9%. The phylogenetic analysis based on the complete chloroplast genome reveals that cv. Wamin is closely related to . This research strengthens the genetic information of both the cv. Wamin and the phylogenetic analyses of Gramineae.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/23802359.2021.1927870DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8158187PMC
May 2021

Dietary Inflammatory Index and Health Outcomes: An Umbrella Review of Systematic Review and Meta-Analyses of Observational Studies.

Front Nutr 2021 19;8:647122. Epub 2021 May 19.

Department of Clinical Epidemiology, Shengjing Hospital of China Medical University, Shenyang, China.

The dietary inflammatory index (DII) is associated with non-communicable disease. We conducted an umbrella review to systematically evaluate meta-analyses of observational studies on DII and diverse health outcomes. We comprehensively searched the PubMed, Web of Science, and Embase databases to identify related systematic reviews and meta-analyses of observational studies. Those investigating the association between DII and a wide range of health outcomes in humans were eligible for inclusion. For each meta-analysis, we estimated the summary effect size by using fixed and random effects models, the 95% confidence interval, and the 95% prediction interval. We assessed heterogeneity, evidence of small-study effects, and excess significance bias. The umbrella review identified 35 meta-analyses assessing associations between DII and various health outcomes: cancer ( = 24), mortality ( = 4), metabolic ( = 4), and other ( = 3). The methodological quality was high or moderate. Of the 35 meta-analyses, we observed highly suggestive evidence for harmful associations between digestive tract cancer, colorectal cancer, overall cancer, pharyngeal cancer, UADT cancer, and CVD mortality. Moreover, 11 harmful associations showed suggestive evidence: hormone-dependent cancer, rectal cancer, colon cancer, breast and prostate cancer, gynecological cancer, breast cancer, ovarian cancer, colorectal cancer, prostate cancer, all-cause mortality, and depression. DII is likely to be associated with harmful effects in multiple health outcomes. Robust randomized controlled trials are warranted to understand whether the observed results are causal. CRD42021218361.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fnut.2021.647122DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8169973PMC
May 2021

Magnetism-mediated targeting hyperthermia-immunotherapy in "cold" tumor with CSF1R inhibitor.

Theranostics 2021 3;11(14):6860-6872. Epub 2021 May 3.

State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 501 Haike Rd, Shanghai 201203, China.

Immunotherapy has profoundly changed the landscape of cancer management and represented the most significant breakthrough. Yet, it is a formidable challenge that the majority of cancers - the so-called "cold" tumors - poorly respond to immunotherapy. To find a general immunoregulatory modality that can be applied to a broad spectrum of cancers is an urgent need. Magnetic hyperthermia (MHT) possesses promise in cancer therapy. We develop a safe and effective therapeutic strategy by using magnetism-mediated targeting MHT-immunotherapy in "cold" colon cancer. A magnetic liposomal system modified with cell-penetrating TAT peptide was developed for targeted delivery of a CSF1R inhibitor (BLZ945), which can block the CSF1-CSF1R pathway and reduce M2 macrophages. The targeted delivery strategy is characterized by its magnetic navigation and TAT-promoting intratumoral penetration. The liposomes (termed TAT-BLZmlips) can induce ICD and cause excessive CRT exposure on the cell surface, which transmits an "eat-me" signal to DCs to elicit immunity. The combination of MHT and BLZ945 can repolarize M2 macrophages in the tumor microenvironment to relieve immunosuppression, normalize the tumor blood vessels, and promote T-lymphocyte infiltration. The antitumor effector CD8 T cells were increased after treatment. This work demonstrated that TAT-BLZmlips with magnetic navigation and MHT can remodel tumor microenvironment and activate immune responses and memory, thus inhibiting tumor growth and recurrence.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7150/thno.57511DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8171105PMC
May 2021

Oral health and caries/gingivitis-associated factors of adolescents aged 12-15 in Shandong province, China: a cross-sectional Oral Health Survey.

BMC Oral Health 2021 06 5;21(1):288. Epub 2021 Jun 5.

Department of Pediatricss Dentistry, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University and Shandong Provincial Key Laboratory of Oral Tissue Regeneration and Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, No.44-1 Wenhua Road West, Jinan, 250012, Shandong, China.

Background: We aimed to analyse the oral health status of adolescents in Shandong province, including dental caries and gingivitis, and their associated factors.

Methods: Adolescents aged 12-15-years in Shandong province were recruited. Caries and gingival status were assessed following the World Health Organisation diagnostic criteria. Information including the sociodemographic, oral hygiene knowledge, attitudes and practices were collected through the questionnaire. Chi-square test and multivariate logistic regression analysis were used to investigate the oral diseases associated factors.

Results: In total, 3868 students (50.2% males) were enrolled. Of these, 39.9% of the participants experienced caries, and 81.7% and 31.3% had calculus and bleeding gingival, respectively. Multivariate logistic regression analysis revealed that there was an association between dental caries and toothaches, dental visits and sleeping troubles caused by oral problems (P < 0.024). A low-frequency of brushing, high sugar consumption and no flossing were more associated with calculus formation and gingival bleeding (P < 0.008).

Conclusion: Compared to caries, worse gingival condition was more prevalent among adolescents in Shandong province. Brushing behaviour is associated with gingivitis, while dental visits and toothaches are associated with caries. Hence, prevention-oriented dental visits and oral hygiene training are strongly recommended to improve oral health status.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12903-021-01640-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8178891PMC
June 2021

Generation of a FTO gene knockout human embryonic stem cell line using CRISPR/Cas9 editing.

Stem Cell Res 2021 May 22;53:102362. Epub 2021 Apr 22.

Bone Marrow Transplantation Center, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310012, Zhejiang, PR China; Institute of Hematology, Zhejiang University, Hangzhou 310012, Zhejiang, PR China; Zhejiang Province Engineering Laboratory for Stem Cell and Immunity Therapy, Hangzhou 310012, Zhejiang, PR China; Liangzhu Laboratory, Zhejiang University Medical Center, 1369 West Wenyi Road, Hangzhou 311121, PR China. Electronic address:

Fat mass and obesity-associated protein (FTO) is the first protein found to have the activity of N6-methyladenosine (m6A) demethylation. It has been reported that FTO was involved in different physiological and pathological processes, including stem cell differentiation, sex determination, tumorigenesis, and progression. To further understand the exact role of FTO in these processes, we generated a FTO knockout human embryonic stem cell (hESC) line by CRISPR/Cas9 mediated gene editing method. This cell line maintained normal karyotype, pluripotency, and trilineage differentiation potential, which are considered as a model for function studies of the FTO protein in hESC self-renewal and differentiation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scr.2021.102362DOI Listing
May 2021

Generation of an ELTD1 knockout human embryonic stem cell line by the iCRISPR/Cas9 system.

Stem Cell Res 2021 May 15;53:102350. Epub 2021 Apr 15.

BoneMarrow Transplantation Center, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310012, Zhejiang, PR China; Institute of Hematology, Zhejiang University, Hangzhou 310012, Zhejiang, PR China; Zhejiang Engineering Laboratory for Stem Cell and Immunotherapy, Hangzhou 310012, Zhejiang, PR China; Zhejiang Laboratory for Systems & Precision Medicine, Zhejiang University Medical Center, Hangzhou 310012, Zhejiang, PR China; Center of Stem Cell and Regenerative Medicine, School of Medicine, Zhejiang University, Hangzhou 310012, PR China. Electronic address:

Human ELTD1 (Epidermal growth factor, latrophilin and seven-transmembrane domain-containing 1), an orphan G-protein-coupled receptor (GPCR) belonging to the adhesion GPCR family, has been reported as a novel regulator of angiogenesis and a potential anti-cancer therapeutic target. However, little is known about the function of ELTD1, especially its undiscovered ligands. In this experiment, an ELTD1 homozygous knockout human embryonic stem cell line, FAHZUe001-A, was generated by the iCRISPR/Cas9 system to achieve a deeper understanding of ELTD1. The FAHZUe001-A was confirmed with normal karyotype, typical undifferentiated morphology, pluripotency and trilineage differentiation potential in vitro.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scr.2021.102350DOI Listing
May 2021

Problematic Smartphone Use during the COVID-19 Pandemic: Its Association with Pandemic-Related and Generalized Beliefs.

Int J Environ Res Public Health 2021 05 26;18(11). Epub 2021 May 26.

Department of Psychology, Faculty of Social Sciences, University of Macau, Macao, China.

Smartphone technologies have played a crucial role in the fight against the COVID-19 pandemic; however, the increased use of smartphones during the pandemic period may expose the general public to a higher risk of problematic smartphone use (PSU). This study aimed to estimate the prevalence of PSU among Chinese community adults and adopted a social-cognitive theory and social axiom framework to evaluate the effects of beliefs on PSU. A Chinese adult sample ( = 616) was obtained through probability sampling via a telephone survey from Macao, China and included 591 smartphone users' data (39.4% men) for formal analysis. The prevalence of PSU was 43.3% in the overall sample, with 41.9% in women, and 45.5% in men. Two types of beliefs derived from the social-cognitive theory, pandemic-related self-efficacy and government efficacy, both showed significant and negative correlations with PSU ( = -0.13 and -0.10, < 0.05). As for the two beliefs from the social axiom framework, reward for application was negatively correlated with PSU ( = -0.10, < 0.05), whereas social cynicism was positively associated with PSU ( = 0.25, < 0.001). Among those four beliefs, social cynicism exerted the most substantial effect on PSU when controlling for demographics. Our findings enriched the understanding of PSU during the pandemic and provided empirical direction regarding cognition-based intervention strategies for reducing PSU.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ijerph18115724DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8198438PMC
May 2021

Study of Microwave-Induced Ag Nanowire Welding for Soft Electrode Conductivity Enhancement.

Micromachines (Basel) 2021 May 27;12(6). Epub 2021 May 27.

State Key Laboratory of Optoelectronic Materials and Technologies, School of Electronics and Information Technology, Sun Yat-Sen University, Guangzhou 510006, China.

Silver nanowire (AgNW)-coated thin films are widely proposed for soft electronics application due to their good conductivity, transparency and flexibility. Here, we studied the microwave welding of AgNW-based soft electrodes for conductivity enhancement. The thermal effect of the microwave to AgNWs was analyzed by dispersing the nanowires in a nonpolar solution, the temperature of which was found to be proportional with the nanowire diameters. AgNWs were then coated on a thin film and welded under microwave heating, which achieved a film conductivity enhancement of as much as 79%. A microwave overheating of AgNWs, however, fused and broke the nanowires, which increased the film resistance significantly. A soft electrode was finally demonstrated using the microwave-welded AgNW thin film, and a 1.13 µA/mM sensitivity was obtained for glucose sensing. Above all, we analyzed the microwave thermal effect on AgNWs to provide a guidance to control the nanowire welding effect, which can be used for film conductivity enhancement and applied for soft and bio-compatible electrodes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/mi12060618DOI Listing
May 2021

Polymer Scaffolds for Biomedical Applications in Peripheral Nerve Reconstruction.

Molecules 2021 May 5;26(9). Epub 2021 May 5.

Department of Orthopedics and Trauma, Peking University People's Hospital, Beijing 100083, China.

The nervous system is a significant part of the human body, and peripheral nerve injury caused by trauma can cause various functional disorders. When the broken end defect is large and cannot be repaired by direct suture, small gap sutures of nerve conduits can effectively replace nerve transplantation and avoid the side effect of donor area disorders. There are many choices for nerve conduits, and natural materials and synthetic polymers have their advantages. Among them, the nerve scaffold should meet the requirements of good degradability, biocompatibility, promoting axon growth, supporting axon expansion and regeneration, and higher cell adhesion. Polymer biological scaffolds can change some shortcomings of raw materials by using electrospinning filling technology and surface modification technology to make them more suitable for nerve regeneration. Therefore, polymer scaffolds have a substantial prospect in the field of biomedicine in future. This paper reviews the application of nerve conduits in the field of repairing peripheral nerve injury, and we discuss the latest progress of materials and fabrication techniques of these polymer scaffolds.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/molecules26092712DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8124340PMC
May 2021

Recent progress towards the transition-metal-catalyzed Nazarov cyclization of alkynes metal carbenes.

Org Biomol Chem 2021 Jun 1. Epub 2021 Jun 1.

College of Sciences and College of Forestry, Henan Agricultural University, Zhengzhou, Henan 450002, China.

In recent years, transition-metal-catalyzed tandem cyclization reactions of alkynes, especially those involving a metal carbene intermediate, have received worthwhile interest, as this type of reaction does not require the use of risky and potentially explosive diazo compounds as starting materials for carbene generation. A significant and general strategy for the stereospecific synthesis of 5-membered cycles is Nazarov cyclization based on the 4π-conrotatory electrocyclization of a conjugated pentadienyl cation to afford a cyclopentenyl cation. In this review, we introduce an overview of recent advances in the transition-metal-catalyzed Nazarov cyclization of alkynes via a metal carbene intermediate, and categorize these reactions according to the structure of the metal carbene. Our aim is to accelerate advancements in this enchanting area of research.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d1ob00744kDOI Listing
June 2021

Characteristics of Central Nervous System (CNS) Involvement in Children With Non-Hodgkin's Lymphoma (NHL) and the Diagnostic Value of CSF Flow Cytometry in CNS Positive Disease.

Technol Cancer Res Treat 2021 Jan-Dec;20:15330338211016372

Medical Oncology Department, Pediatric Oncology Center, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, China.

Objective: To investigate the characteristics of central nervous system (CNS) involvement in children with non-Hodgkin's lymphoma (NHL) and the value of flow cytometry (FC) in the diagnosis of CNS disease in pediatric NHL.

Methods: The data of 56 newly diagnosed pediatric NHL patients with CNS involvement (CNS+/mass, CNS+/palsy, CNS+/CSF) were analyzed. The proportions and formats of CNS disease in different pathological types were compared. In addition, FC and conventional cytology (CC) of cerebrospinal fluid (CSF) were carried out in 383 newly diagnosed NHL cases.

Results: A total of 383 children with NHL were enrolled. Among these patients, 56 (14.6%) were diagnosed with positive CNS involvement (CNS+), 33 had bulky disease (tumor diameter >10 cm), 32 had bone marrow invasion, 32 had lactate dehydrogenase levels >1000 U/L, and 25 had invasion of more than 4 organs at the time of diagnosis. There were 14 patients with T lymphoblastic lymphoma (T-LBL), 9 with B lymphoblastic lymphoma (B-LBL), 26 with Burkitt's lymphoma (BL), and 2 with Epstein-Barr virus-positive diffuse large B cell lymphoma (EBV + DLBCL). Among the 56 CNS+ patients, 35 were CSF-positive (CSF+); there were 2 patients who were CSF+ via CC detection and 35 who were CSF+ via FC detection. The difference between CC and FC was statistically significant ( < 0.01). In the T-LBL group, 14 patients were CNS+/CSF, and in the B-LBL group, 8 were CNS+/mass. In the BL group, 22 patients were CNS+/mass and 15 were CNS+/CSF. In the anaplastic large-cell lymphoma group, 5 patients were CNS+/mass. Nine of the 56 CNS+ patients had events. The 2-year overall survival rate was 87% ± 0.046%, and the 2-year event-free survival rate was 76.2% ± 0.07%.

Conclusion: CNS+ diagnoses were more common in pediatric NHL patients with bulky disease and/or bone marrow involvement and/or involvement of more than 4 organs at the time of diagnosis, and they were also common in the EBV + DLBCL and BL groups. FC of CSF showed important clinical significance in the diagnosis of CNS disease in pediatric NHL patients, and it can be used to significantly improve the CNS+ detection rate.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/15330338211016372DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8173989PMC
June 2021

Molecular characterization of ctDNA from Chinese patients with advanced gastric adenocarcinoma reveals actionable alterations for targeted and immune therapy.

J Mol Med (Berl) 2021 May 31. Epub 2021 May 31.

Gastrointestinal Cancer Center, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Peking University Cancer Hospital and Institute, Beijing, China.

Circulating tumor DNA (ctDNA) is considered an ideal sample type for genotyping patients with advanced unresectable cancer to inform treatment decision. It may better capture tumor heterogeneity, especially in gastric adenocarcinoma (GAC). However, there exists little evidence regarding genomic profiling of Chinese advanced GAC patients from ctDNA. Blood samples were obtained from 200 advanced GAC patients. Next-generation sequencing (NGS) was performed on ctDNA using a validated 150-gene panel. Blood tumor mutation burden (bTMB) was calculated according to the NGS results. Blood microsatellite instability (bMSI) status was determined by targeted sequencing of 100 microsatellite loci. One hundred sixty-nine (84.5%) patients carried at least one genomic alteration and 138 (69%) patients had at least one deleterious or likely deleterious alteration (del-alteration). The clonal fraction of del-alterations was higher than that of non-del-alterations (80.1% vs 54.5%, P < 0.0001). The most frequently altered genes were TP53 (38%), LRP1B (20%), MYC (13.5%), ERBB2 (12.5%), and KRAS (11.5%). The alterations were most enriched in the TP53/cell cycle (52%) and the RTK-Ras-MAPK pathway (51.5%). The median bTMB was two (range 0 to 42). Eight patients were identified to be high bMSI, with higher median bTMB than the blood microsatellite stable (bMSS) patients (15 vs 2, P = 0.0062). Patients harboring del-alterations of the DDR pathway had significantly higher percentages of high bTMB and bMSI-H patients than the wild-type subgroup (61.1% vs 6.5%, P < 0.0001; 33.3% vs 1.7%, P = 0.0002). A total of 45.5% cases harbored at least one potentially actionable alteration and one patient achieved complete response after receiving matched targeted therapy. Our study uncovered the molecular characterization of Chinese patients with advanced GAC from ctDNA, including genomic alteration, bTMB, and bMSI status. The findings suggested that targeted NGS-based ctDNA analysis may help inform the clinical decision in advanced GAC. KEY MESSAGES: We report the molecular profiling of the largest Chinese advance stage GACs cohort using a CLIA-certified ctDNA assay. Potentially actionable genomic alterations were identified in 45.5% of patients, suggesting clinical utility for ctDNA NGS in advance stage GACs. There was evidence of clinical benefit in one GAC patient with MET amplification treated with MET inhibitor.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00109-021-02093-zDOI Listing
May 2021

/ Are Essential for the Formation of Anther Cuticle and Pollen Exine in Cotton ( L.).

Front Plant Sci 2021 13;12:667739. Epub 2021 May 13.

State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, China.

Glycerol-3-phosphate acyltransferases (GPATs), critical for multiple biological processes like male fertility, have been extensively characterized. However, their precise functions and underlying regulatory mechanism in cotton anther development are unclear. This research demonstrated the importance of / (a paralogs pair on A12/D12 sub-chromosome of cotton) to regulate the degradation of tapetum, anther cuticle formation, and pollen exine development. and exhibited specifically detected transcripts in tapetum and pollen exine during the early anther developmental stages. GhGPAT12/25 are sn-2 glycerol-3-phosphate acyltransferases and can transfer the acyl group of palmitoyl-CoA to glycerol-3-phosphate (G3P). CRISPR/Cas9-mediated knockout identified the functional redundancy of and . Knockout of both genes caused completely male sterility associated with abnormal anther cuticle, swollen tapetum, and inviable microspores with defective exine and irregular unrestricted shape. RNA-seq analysis showed that the loss of function of / affects the processes of wax metabolic, glycerol monomer biosynthesis, and transport. Consistently, cuticular waxes were dramatically reduced in mutant anthers. Yeast one-hybrid system (Y1H), virus-induced gene silencing (VIGS), and dual-luciferase (LUC) assays illustrated that GhMYB80s are likely to directly activate the expression of /. This study provides important insights for revealing the regulatory mechanism underlying anther development in cotton.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpls.2021.667739DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8155372PMC
May 2021

Salusin-β Mediates High Glucose-Induced Inflammation and Apoptosis in Retinal Capillary Endothelial Cells via a ROS-Dependent Pathway in Diabetic Retinopathy.

Diabetes Metab Syndr Obes 2021 21;14:2291-2308. Epub 2021 May 21.

Department of Ophthalmology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, People's Republic of China.

Background: Diabetic retinopathy (DR) is characterized by retinal vascular endothelial cell death and vascular inflammation, which are microvascular complications of diabetes mellitus (DM). Salusin-β, a newly identified peptide, is closely associated with hypertension, atherosclerosis and diabetic cardiomyopathy. However, the exact role of salusin-β in high glucose (HG)-induced retinal capillary endothelial cell (REC) inflammation and apoptosis remains unclear.

Patients And Methods: A total of 60 patients with type 2 diabetes and 20 healthy controls were included in this study. Based on fundus fluorescein angiography findings, the diabetic patients were divided into three subgroups: diabetes without retinopathy (DWR), non-proliferative DR (NPDR) and proliferative DR (PDR). Serum salusin-β levels were measured by enzyme-linked immunosorbent assay. Human RECs (HRECs) were cultured in normal glucose (NG) and HG medium with or without salusin-β. Salusin-β expression was analysed by Western blotting and immunofluorescence staining. Expression of the pro-inflammatory cytokines MCP-1, IL-1β, TNF-α, and VCAM-1 was analysed by Western blotting. Reactive oxygen species (ROS) production was measured with 2',7'-dichlorofluorescein diacetate (DCFH-DA). Cell apoptosis rates were determined by flow cytometry. The levels of p38, JNK, p-p38, and p-JNK and the apoptosis-related proteins cleaved caspase-3, Bax, and cl2 were analysed by Western blotting.

Results: Serum salusin-β levels were higher in diabetic patients than in healthy controls (p = 0.0027), especially in patients with NPDR and PDR (both p<0.01). HG upregulated salusin-β expression in HRECs in a time-dependent manner. Salusin-β exacerbated inflammation and apoptosis, upregulated intracellular ROS production in HG-induced HRECs, and activated ROS-dependent JNK and p38 MAPK signalling, while knockdown of salusin-β suppressed these effects.

Conclusion: Our findings indicate that salusin-β can promote inflammation and apoptosis via ROS-dependent JNK and p38 MAPK signalling in HG-induced HRECs and could be a therapeutic target for DR.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2147/DMSO.S301157DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8153208PMC
May 2021

Association of hidradenitis suppurativa with Crohn's disease.

World J Clin Cases 2021 May;9(15):3506-3516

Department of Colorectal Surgery, TheAffiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of Chinese Medicine, Nanjing 210029, Jiangsu Province, China.

Hidradenitis suppurativa (HS) is a chronic inflammatory skin disorder characterized by recurrent nodules, abscesses, and sinus tracts. Crohn's disease (CD) is characterized by inflammation of the entire digestive tract and belongs to the group of inflammatory bowel diseases, and there are many extraintestinal manifestations, among which hidradenitis suppurativa is one of the rare extraintestinal manifestations. There appears to be a strong association between CD and HS based on clinical and histological similarities (sinus tract development, granulomatous inflammation, and scarring), intersections in pathogenesis (genetic loci, immune dysregulation mechanisms, and microbiome changes), and commonality in treatment. In this review, we summarize recent studies on the association between HS and CD.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.12998/wjcc.v9.i15.3506DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8130070PMC
May 2021

Identification of Vitamin D-related gene signature to predict colorectal cancer prognosis.

PeerJ 2021 13;9:e11430. Epub 2021 May 13.

Hubei Clinical Center and Key Lab of Intestinal and Colorectal Diseases, Wuhan, China.

Colorectal cancer (CRC) is one of the most common malignant carcinomas worldwide with poor prognosis, imposing an increasingly heavy burden on patients. Previous experiments and epidemiological studies have shown that vitamin D and vitamin D-related genes play a vital role in CRC. Therefore, we aimed to construct a vitamin D-related gene signature to predict prognosis in CRC. The CRC data from The Cancer Genome Atlas (TCGA) was performed as the training set. A total of 173 vitamin D-related genes in the TCGA CRC dataset were screened, and 17 genes associated with CRC prognosis were identified from them. Then, a vitamin D-related gene signature consisting of those 17 genes was established by univariate and multivariate Cox analyses. Moreover, four external datasets (GSE17536, GSE103479, GSE39582, and GSE17537) were used as testing set to validate the stability of this signature. The high-risk group presented a significantly poorer overall survival than low-risk group in both of training set and testing sets. Besides, the areas under the curve (AUCs) for signature on OS in training set at 1, 3, and 5 years were 0.710, 0.708, 0.710 respectively. The AUCs of the ROC curve in GSE17536 for 1, 3, and 5 years were 0.649, 0.654, and 0.694. These results indicated the vitamin D-related gene signature model could effectively predict the survival status of CRC patients. This vitamin D-related gene signature was also correlated with TNM stage in CRC clinical parameters, and the higher risk score from this model was companied with higher clinical stage. Furthermore, the high accuracy of this prognostic signature was validated and confirmed by nomogram model. In conclusion, we have proposed a novel vitamin D-related gene model to predict the prognosis of CRC, which will help provide new therapeutic targets and act as potential prognostic biomarkers for CRC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7717/peerj.11430DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8126261PMC
May 2021

Therapeutic effect of various ginsenosides on rheumatoid arthritis.

BMC Complement Med Ther 2021 May 25;21(1):149. Epub 2021 May 25.

Department of Pharmaceutics, School of Pharmacy, Fudan University & Key Laboratory of Smart Drug Delivery, Ministry of Education, Shanghai, 201203, China.

Background: Rheumatoid arthritis (RA) is an autoimmune disease which causes disability and threatens the health of humans. Therefore, it is of great significance to seek novel effective drugs for RA. It has been reported that various ginsenoside monomers are able to treat RA. However, it is still unclear which ginsenoside is the most effective and has the potential to be developed into an anti-RA drug.

Methods: The ginsenosides, including Rg1, Rg3, Rg5, Rb1, Rh2 and CK, were evaluated and compared for their therapeutic effect on RA. In in vitro cell studies, methotrexate (MTX) and 0.05% dimethyl sulfoxide (DMSO) was set as a positive control group and a negative control group, respectively. LPS-induced RAW264.7 cells and TNF-α-induced HUVEC cells were cultured with MTX, DMSO and six ginsenosides, respectively. Cell proliferation was analyzed by MTT assay and cell apoptosis was carried out by flow cytometry. CIA mice model was developed to evaluate the therapeutic efficacy of ginsenosides. The analysis of histology, immunohistochemistry, flow cytometry and cytokine detections of the joint tissues were performed to elucidate the action mechanisms of ginsenosides.

Results: All six ginsenosides showed good therapeutic effect on acute arthritis compared with the negative control group, Ginsenoside CK provided the most effective treatment ability. It could significantly inhibit the proliferation and promote the apoptosis of RAW 264.7 and HUVEC cells, and substantially reduce the swelling, redness, functional impairment of joints and the pathological changes of CIA mice. Meanwhile, CK could increase CD8 + T cell to down-regulate the immune response, decrease the number of activated CD4 + T cell and proinflammatory M1-macrophages, thus resulting in the inhibition of the secretion of proinflammatory cytokine such as TNF-α and IL-6.

Conclusion: Ginsenoside CK was proved to be a most potential candidate among the tested ginsenosides for the treatment of RA, with a strong anti-inflammation and immune modulating capabilities.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12906-021-03302-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8145820PMC
May 2021

Survival models to support shared decision-making about advance care planning for people with advanced stage cystic fibrosis.

BMJ Open Respir Res 2021 05;8(1)

Feinstein Institutes for Medical Research, Northwell Health, Manhasset, NY, USA.

Background: For people with advanced stage cystic fibrosis (CF), tailored survival estimates could facilitate preparation for decision-making in the event of acutely deteriorating respiratory function.

Methods: We used the US CF Foundation national database (2008-2013) to identify adult people with incident advanced stage CF (forced expiratory volume in 1 s (FEV1) ≤45% predicted). Using the lasso method for variable selection, we divided the dataset into training and validation samples (2:1), and developed two multivariable Cox proportional hazards models to calculate probabilities of survival from baseline (T0 model), and from 1 year after (T12 model). We also performed Kaplan-Meier survival analyses.

Results: 4752 people were included. For the T0 model, FEV1; insurance; non-invasive ventilation; supplemental oxygen; colonisation; cirrhosis; depression; dialysis; current smoking; unclassifiable mutation class and cumulative CF exacerbations predicted increased mortality. Baseline transplant evaluation status of 'accepted, on waiting list' predicted decreased mortality. For the T12 model, interim decrease in FEV1 >10%, and pulmonary exacerbations additionally increased predicted mortality. Lung transplantation was associated with lower mortality. Of the 4752, 93.5%, 86.4%, 79.7% and 73.9% survived to 1, 2, 3 and 4 years, respectively, without considering any confounding variables. The models had moderate predictive ability indicated by the area under the time-dependent receiver operating characteristic curve (0.787, 95% CI 0.769 to 0.794 for T0 model; and 0.779, 95% CI 0.767 to 0.797 for T12 model).

Conclusion: We have developed models predicting survival in people with incident advanced stage CF, which can be reapplied over time to support shared decision-making about end-of-life treatment choices and lung transplantation. These estimates must be updated as data become available regarding long-term outcomes for people treated with CF transmembrane conductance regulator modulators.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1136/bmjresp-2020-000794DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8149438PMC
May 2021

Low-dose theophylline in addition to ICS therapy in COPD patients: A systematic review and meta-analysis.

PLoS One 2021 24;16(5):e0251348. Epub 2021 May 24.

Department of Intensive Care Unit, Lanzhou University, The First Hospital of Lanzhou University, Lan Zhou, Gansu Province, China.

Background: A synergism has been reported between theophylline and corticosteroids, wherein theophylline increases and restores the anti-inflammatory effect of inhaled corticosteroids (ICS) by enhancing histone deacetylase-2 (HDAC) activity. Several studies have explored the efficacy of low-dose theophylline plus ICS therapy on chronic obstructive pulmonary disease (COPD) but the results are discrepant.

Method: We conducted searches in electronic database such as PubMed, Web Of Science, Cochrane Library, and Embase to find out original studies. Stata/SE 15.0 was used to perform all data analysis.

Result: A total of 47,556 participants from 7 studies were included in our analysis and the sample size of each study varied from 24 to 10,816. Theophylline as an add-on therapy to ICS was not associated with the reduction of COPD exacerbations (HR: 1.08, 95% CI: 0.97 to 1.19, I2 = 95.2%). Instead, the theophylline group demonstrated a higher hospitalization rate (HR: 1.12, 95% CI: 1.10 to 1.15, I2 = 20.4%) and mortality (HR: 1.19, 95% CI: 1.14 to 1.25, I2 = 0%). Further, the anti-inflammatory effect of low-dose theophylline as an adjunct to ICS on COPD was controversial. Besides, the theophylline group showed significant improvement in lung function compared with the non-theophylline group.

Conclusion: Based on current evidence, low-dose theophylline as add-on therapy to ICS did not reduce the exacerbation rate. Instead, the hospitalization rate and mortality increased with theophylline. Thus, we do not recommend adding low-dose theophylline to ICS therapy in COPD patients.

Trial Registration: PROSPERO Registration CRD42021224952.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0251348PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8143407PMC
May 2021

Effects of N-terminal modifications on the stability of antimicrobial peptide SAMP-A4 analogues against protease degradation.

J Pept Sci 2021 May 24:e3352. Epub 2021 May 24.

College of Biological Engineering, Henan University of Technology, Zhengzhou, China.

Infections with multidrug-resistant (MDR) pathogens are increasingly concerning for public health. Synthesized antimicrobial peptide A4 (SAMP-A4), a peptide computationally designed by our research team, is a potential drug candidate. However, the antimicrobial peptide SAMP-A4 is easily degraded in serum. To obtain SAMP-A4 analogues with high biostability, chemical modifications at its N-terminus, including fatty acid conjugation, glycosylation and PEGylation, were carried out. The results showed that the introduction of hydrophobic fatty acids at the N-terminus of SAMP-A4 is better than hydrophilic glycosylation and PEGylation. With increasing fatty acid chain length, the stability of SAMP-A4 analogues in serum and trypsin solutions is significantly improved, and the activities against MDR bacteria and Candida are significantly enhanced. There is no obvious change in haemolysis even when hexanoic acid is coupled with SAMP-A4, so the resulting analogue SAMP-A4-C6, SAMP-A4 conjugated with hexanoic acid, is the most likely of the analogues to become a drug.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/psc.3352DOI Listing
May 2021

A dual-photoelectrode photofuel cell based self-powered aptasensor using a multimeter as a direct visual readout strategy.

Chem Commun (Camb) 2021 Jun;57(48):5973-5976

Key Laboratory of Modern Agriculture Equipment and Technology, School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, P. R. China.

A self-powered aptasensor based on a dual-photoelectrode photofuel cell, whose maximum power density and open circuit potential respectively reached 10.77 μW cm-2 and 0.664 V, was proposed combined with a digital multimeter as a direct visual readout strategy, providing a meaningful perspective for miniaturized, implantable, and on-site sensors.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d1cc02078aDOI Listing
June 2021

Significantly hypoglycemic effect of a novel functional bread rich in mulberry bark and improving the related functions of liver, pancreas, and kidney, on T2D mice.

Food Sci Nutr 2021 May 8;9(5):2468-2482. Epub 2021 Mar 8.

School of Biology and Basic Medical Sciences Soochow University Suzhou China.

To develop a novel functional food with lowering blood glucose for diabetics, the mixed bread containing mulberry branch bark powder (MBBP) was used for the oral administration of the type 2 diabetic (T2D) mice induced by streptozocin (STZ), high-fat and high-sugar diet for 7 weeks. 5%, 10%, and 15% MBBP bread diets had a significatively positive influence on the biochemical indicators, histological examination, and immunohistochemistry observations in T2D mice. The 15% MBBP-rich bread diet evidently retarded loss weight of T2D mice, and decreased in FBG by about 55% and in glycosylated hemoglobin (HbA1c) levels by about 30%. Its glucose tolerance and serum insulin levels were very close to normal level. The abnormal lipid metabolism and insulin-related index of T2D mice in both the 10% and 15% MBBP bread diet groups were partly reversed. The Western blotting results showed that the expression levels of key proteins in PI3K/AKT signaling pathway were decreased and expression levels of immunoproteins PPARγ, TNF-α, P65, and IL6 were increased. In general, oral MBBP bread diets effectively restored some functions and repaired damage to the pancreas, liver, and kidney in T2D mice. Therefore, MBBP is potential to develop into a novel functional food additive with significantly hypoglycemic effect.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/fsn3.2189DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8116878PMC
May 2021

Integrated Pharmacological Analysis on the Mechanism of Fuyou Formula in Treating Precocious Puberty.

Front Pharmacol 2021 7;12:649732. Epub 2021 May 7.

Department of Pharmacy, National Center for Children Health, Beijing Children's Hospital, Capital Medical University, Beijing, China.

Fu-you formula (FY), a Traditional Chinese Medicine (TCM) formula composed of 12 herbs, as an in-hospital preparation, has been used treat to precocious puberty (PP) for decades. However, the lack of phytochemical characterization and mechanism of FY remains the main limitation for its spreading. In this study, we analyze the components and mechanisms of FY in PP, based on the integrated pharmacology. Investigated main constituents, targets, pathways of FY by using an integrative pharmacology, and recognized main constituents by HPLC-MS/MS. Then, observed the levels of Follicle-stimulating hormone (FSH), luteinizing hormone (LH), and estrogen (E) in danazol-induced PP in Sprague-Dawley (SD) rats. Lastly, retrospective study analyzed the clinical data of 575 patients who were diagnosed PP, treated by the FY, and followed-up in our hospital from 2014-2020. The result that total of 116 important candidate targets were selected based on pharmacological analysis. Selected the top 10 values key targets such as the estrogen receptor alpha (ESR1) and insulin-like growth factor 1 (IGF1), were localized and the related gene functions were determined. Gene functions were associated with biological regulation, a cellular process, or signaling pathway, such as the Estrogen signaling pathway, MAPK signaling pathway and PI3K-Akt signaling pathway. By recognizing the five compounds related to the ESR1 and IGF1, which are Quercetin, kaempferol, Luteolin, Apigenin, and Emodin. The results of the molecular docking study further showed that the flavonoids had a strong binding affinity for ESR1 and IGF1 after docking into the crystal structure. The results showed that the FY could effectively reduce E, LH, and FSH levels in SD rats. Furthermore, the results of the retrospective analysis of medical records showed that the FY could remarkably reduce E levels in girls with PP.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fphar.2021.649732DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8138182PMC
May 2021

Annexin A1 protects against cerebral ischemia-reperfusion injury by modulating microglia/macrophage polarization via FPR2/ALX-dependent AMPK-mTOR pathway.

J Neuroinflammation 2021 May 22;18(1):119. Epub 2021 May 22.

Department of Neurosurgery, Xuanwu Hospital, Capital Medical University, 45 Changchun Street, Beijing, 100053, China.

Background: Cerebral ischemia-reperfusion (I/R) injury is a major cause of early complications and unfavorable outcomes after endovascular thrombectomy (EVT) therapy in patients with acute ischemic stroke (AIS). Recent studies indicate that modulating microglia/macrophage polarization and subsequent inflammatory response may be a potential adjunct therapy to recanalization. Annexin A1 (ANXA1) exerts potent anti-inflammatory and pro-resolving properties in models of cerebral I/R injury. However, whether ANXA1 modulates post-I/R-induced microglia/macrophage polarization has not yet been fully elucidated.

Methods: We retrospectively collected blood samples from AIS patients who underwent successful recanalization by EVT and analyzed ANXA1 levels longitudinally before and after EVT and correlation between ANXA1 levels and 3-month clinical outcomes. We also established a C57BL/6J mouse model of transient middle cerebral artery occlusion/reperfusion (tMCAO/R) and an in vitro model of oxygen-glucose deprivation and reoxygenation (OGD/R) in BV2 microglia and HT22 neurons to explore the role of Ac2-26, a pharmacophore N-terminal peptide of ANXA1, in regulating the I/R-induced microglia/macrophage activation and polarization.

Results: The baseline levels of ANXA1 pre-EVT were significantly lower in 23 AIS patients, as compared with those of healthy controls. They were significantly increased to the levels found in controls 2-3 days post-EVT. The increased post-EVT levels of ANXA1 were positively correlated with 3-month clinical outcomes. In the mouse model, we then found that Ac2-26 administered at the start of reperfusion shifted microglia/macrophage polarization toward anti-inflammatory M2-phenotype in ischemic penumbra, thus alleviating blood-brain barrier leakage and neuronal apoptosis and improving outcomes at 3 days post-tMCAO/R. The protection was abrogated when mice received Ac2-26 together with WRW4, which is a specific antagonist of formyl peptide receptor type 2/lipoxin A4 receptor (FPR2/ALX). Furthermore, the interaction between Ac2-26 and FPR2/ALX receptor activated the 5' adenosine monophosphate-activated protein kinase (AMPK) and inhibited the downstream mammalian target of rapamycin (mTOR). These in vivo findings were validated through in vitro experiments.

Conclusions: Ac2-26 modulates microglial/macrophage polarization and alleviates subsequent cerebral inflammation by regulating the FPR2/ALX-dependent AMPK-mTOR pathway. It may be investigated as an adjunct strategy for clinical prevention and treatment of cerebral I/R injury after recanalization. Plasma ANXA1 may be a potential biomarker for outcomes of AIS patients receiving EVT.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12974-021-02174-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8140477PMC
May 2021