Publications by authors named "Meng Sun"

311 Publications

An Image-based Dynamic High-throughput Analysis of Adherent Cell Migration.

Bio Protoc 2021 Mar 20;11(6). Epub 2021 Mar 20.

Rheumatology Unit, Department of Medicine, Karolinska University Hospital Center for Rheumatology, Academic Specialist Centre, Stockholm, Sweden.

In this protocol, we describe a method to monitor cell migration by live-cell imaging of adherent cells. Scratching assay is a common method to investigate cell migration or wound healing capacity. However, achieving homogenous scratching, finding the optimal time window for end-point analysis and performing an objective image analysis imply, even for practiced and adept experimenters, a high chance for variability and limited reproducibility. Therefore, our protocol implemented the assessment for cell mobility by using homogenous wound making, sequential imaging and automated image analysis. Cells were cultured in 96-well plates, and after attachment, homogeneous linear scratches were made using the IncuCyte WoundMaker. The treatments were added directly to wells and images were captured every 2 hours automatically. Thereafter, the images were processed by defining a scratching mask and a cell confluence mask using a software algorithm. Data analysis was performed using the IncuCyte Cell Migration Analysis Software. Thus, our protocol allows a time-lapse analysis of treatment effects on cell migration in a highly reliable, reproducible and re-analyzable manner.
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http://dx.doi.org/10.21769/BioProtoc.3957DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8032497PMC
March 2021

Fabrication of MOF-derived tubular InO@SnInS hybrid: Heterojunction formation and promoted photocatalytic reduction of Cr(VI) under visible light.

J Colloid Interface Sci 2021 Mar 24;596:278-287. Epub 2021 Mar 24.

State Key Laboratory of Photocatalysis on Energy and Environment, Research Institute of Photocatalysis, Fuzhou University, Fuzhou 350116, PR China. Electronic address:

Tubular InO@SnInS hierarchical hybrid photocatalyst was firstly fabricated by a two-step method. The morphology and composition were characterized by scanning electron microscope (SEM), X-ray photoelectron spectroscopy (XPS), and X-ray diffraction (XRD). The XRD results show that the obtained InO microtubes were highly crystallized, while the SnInS flakes prepared at low temperature were poorly crystallized. The SEM image of the hybrid shows that numerous SnInS nanoflakes were assembled over the surface of InO microtubes. InO served as dispersing-templates have reduced the agglomeration of SnInS flakes. Meanwhile, the heterojunctions formed at the interfaces between InO and SnInS could facilitate the interfacial charge transfer, as well as promote the photocatalytic activity of the hybrid. In the treatment of Cr(VI)-containing wastewater, the InO@SnInS hybrid not only exhibited strong adsorption ability, but also showed remarkably enhanced photocatalytic activity compared with pure SnInS. The photocatalytic reaction constant k for InO@SnInS was approximately 2.54 times higher than that of SnInS. The efficient activity of this hybrid photocatalyst should be ascribed to the promoted separation efficiency of electron/hole pairs, which was proved by the following three-dimensional excitation-emission matrix fluorescence spectra (3D EEMs), photocurrent responds, and EIS characterizations.
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http://dx.doi.org/10.1016/j.jcis.2021.02.121DOI Listing
March 2021

Plug-in tubes allow tunable oil removal, droplet packing, and reaction incubation for time-controlled droplet-based assays.

Biomicrofluidics 2021 Mar 5;15(2):024108. Epub 2021 Apr 5.

Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109, USA.

Here, we report a unique microfluidic technique that utilizes a membrane filter and plug-in tubes to remove oil and pack water-in-oil droplets for controlled incubation of droplet-based assays. This technique could be modularly incorporated into most droplet-generation devices without a need to alter the original designs. Our results show that removing excess oil to form tightly packed droplets allows for extended and controllable incubation for droplets traveling in microchannels. The efficiency of this technique was evaluated and confirmed using a time-dependent enzyme assay with a fluorometric readout. The system is also readily generalizable to control inter-droplet distance, crucial for studying droplet communication and pattern formation.
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http://dx.doi.org/10.1063/5.0047924DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8024030PMC
March 2021

Identification of NY-ESO-1 Specific Murine T Cell Receptors With Distinct Recognition Pattern for Tumor Immunotherapy.

Front Immunol 2021 23;12:644520. Epub 2021 Mar 23.

Research Network of Immunity and Health (RNIH), Beijing Institutes of Life Science, Chinese Academy of Sciences, Beijing, China.

New York esophageal squamous cell carcinoma 1 (NY-ESO-1) is a promising target for T-cell receptor-engineered T cell (TCR-T) therapy, and targeting the human leukocyte antigen (HLA)-A2 restricted NY-ESO-1 epitope has yielded remarkable clinical benefits in the treatment of multiple advanced malignancies. Herein, we report the identification of two NY-ESO-1 epitope-specific murine TCRs obtained from HLA-A0201 transgenic mice. NY-ESO-1 specific TCRs were isolated after vaccinating HLA-A2 transgenic mice with epitope peptides. HZ6 and HZ8 TCRs could specifically bind to NY-ESO-1/HLA-A2 and were capable of cytokine secretion with engineered Jurkat T cells and primary T cells upon recognition with K562 target cells expressing the single-chain trimer (SCT) of NY-ESO-1/HLA-A2. The reactivity profiles of the HZ6 and HZ8 TCRs were found to be distinct from one another when co-cultured with K562 target cells carrying alanine-substituted NY-ESO-1 SCTs. The binding characterization revealed that the recognition pattern of the HZ6 TCR to NY-ESO-1/HLA-A2 was substantially different from the widely used 1G4 TCR. These findings would broaden the understanding of immunogenicity of the NY-ESO-1, and the two identified TCRs may serve as promising candidates for the future development of TCR-T therapy for tumors.
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http://dx.doi.org/10.3389/fimmu.2021.644520DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8021954PMC
March 2021

(9βH)- and 17-Nor-Pimaranes from .

J Nat Prod 2021 Mar 26. Epub 2021 Mar 26.

Department of Pharmaceutical Sciences, College of Pharmacy, University of Illinois at Chicago, Chicago, Illinois 60612, United States.

Eleven pimarane-type diterpenoids were isolated from the tubers of , including three new compounds, icacinlactone M (), icacinlactone H 2--β-d-glucopyranoside (), and icacinlactone N 3--β-d-glucopyranoside (), together with an artifact of acrenol (). Among the known structures, icacinlactone A (), icacinlactone B (), icacinlactone H (), 12-hydroxyicacinlactone A (), 14α-methoxyhumirianthol (), and annonalide () are reported from for the first time, whereas icacinol () has previously been found in this plant. Icacinol, 14α-methoxyhumirianthol, and annonalide displayed moderate cytotoxic activity in a panel of human cancer cell lines.
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http://dx.doi.org/10.1021/acs.jnatprod.9b01131DOI Listing
March 2021

EMLP: short-term gas load forecasting based on ensemble multilayer perceptron with adaptive weight correction.

Math Biosci Eng 2021 Feb;18(2):1590-1608

College of Computer Science and Technology, Shanghai University of Electric Power, Shanghai 201306, China.

This paper tackles a recent challenge in smart city that how to improve the accuracy of short-term natural gas load forecasting. Existing works on natural gas forecasting mostly reply on a combined forecasting model by simply integrating several single-forecasting models. However, due to the existence of redundant single-forecasting models, these works may not attain a higher prediction accuracy. To address the problem, we design a new natural gas load forecasting scheme based on ensemble multilayer perceptron (EMLP) with adaptive weight correction. Our method firstly normalizes multi-source data as original data set, which is further segmented by a window model. Then, the abnormal data is removed and subsequently interpolated to form a complete normalized data set. Furthermore, we integrate a series of multilayer perceptron (MLP) network to construct an ensemble forecasting model. An adaptive weight correction function is introduced to dynamically modify the weight of the previous predicted result. Since the correction function can match well the volatility characteristics of load data, the prediction accuracy is significantly improved. Extensive experiments demonstrate that our method outperforms existing state-of-the-art load forecasting schemes in terms of the prediction accuracy and stability.
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http://dx.doi.org/10.3934/mbe.2021082DOI Listing
February 2021

Changes in psychotic-like experiences and related influential factors in technical secondary school and college students during COVID-19.

Schizophr Res 2021 Mar 13;231:3-9. Epub 2021 Mar 13.

Department of Social Psychiatry, the Affiliated Brain Hospital of Guangzhou Medical University (Guangzhou Huiai Hospital), Guangzhou, China. Electronic address:

Objective: Although students have been found to be at high risk of distress during the COVID-19 pandemic, little is known about the pandemic's impact on psychotic-like experiences (PLEs). We conducted a study in technical secondary school and college students before and during the pandemic to explore changes in PLEs and relevant influential factors.

Methods: A total of 938 students completed both waves of the survey through electronic questionnaires. PLEs were assessed using the 15-item Positive Subscale of the Community Assessment of Psychic Experiences (CAPE-P15). Childhood trauma, perceived stress, resilience, and demographic factors were evaluated at baseline, and psychological status was measured during the pandemic.

Results: The overall CAPE-P15 scores significantly decreased during the pandemic. Students with persistent PLEs showed the most severe COVID-19 related psychological symptoms, followed by new-onset and remitted individuals; those without PLEs exhibited the mildest symptoms (all p < .001). A single parent family (OR = 4.707), more childhood trauma (OR = 1.056), and a higher family income (OR = 1.658) were predictive of new-onset PLEs during the pandemic, while better resilience was a protective factor, associated with remission of previous PLEs (OR = 0.932).

Conclusions: Despite a downward trend in the prevalence of PLEs during the pandemic, PLEs predict greater serious psychological impact due to COVID-19, especially for students with persistent PLEs. Interventions that cultivate students' resilience are urgently needed to reduce PLEs and improve mental health, especially for students from single parent households or those who have experienced childhood trauma.
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http://dx.doi.org/10.1016/j.schres.2021.02.015DOI Listing
March 2021

The TSPO-specific Ligand PK11195 Protects Against LPS-Induced Cognitive Dysfunction by Inhibiting Cellular Autophagy.

Front Pharmacol 2020 29;11:615543. Epub 2021 Jan 29.

Shandong Provincial Medicine and Health Key Laboratory of Clinical Anesthesia, School of Anesthesiology, Weifang Medical University, Weifang, China.

Perioperative neurocognitive disorders (PND) is a common postoperative neurological complication. Neuroinflammation is a major cause that leads to PND. Autophagy, an intracellular process of lysosomal degradation, plays an important role in the development and maintenance of nervous system. PK11195 is a classic translocator protein (TSPO) ligand, which can improve the cognitive function of rats. In this study, we evaluate the protective effect of PK11195 on the learning and memory of rats. A rat model of lipopolysaccharide (LPS)-induced cognitive dysfunction was established by intraperitoneal injection of LPS. Morris Water Maze (MWM), Western blot, qRT-PCR, confocal microscopy and transmission electron microscopy (TEM) were used to study the role of TSPO-specific ligand PK11195 in LPS-activated mitochondrial autophagy in rat hippocampus. We found that PK11195 ameliorated LPS-induced learning and memory impairment, as indicated by decreased escape latencies, swimming distances and increased target quadrant platform crossing times and swimming times during MWM tests. TSPO, ATG7, ATG5, LC3B and p62 protein and mRNA expression increased in the hippocampus of PND model rats. The hippocampal microglia of PND model rats also have severe mitochondrial damage, and a large number of autophagosomes and phagocytic vesicles can be seen. PK11195 pretreatment significantly decreased the expression of TSPO, ATG7, ATG5, LC3B and p62 protein and mRNA, as well as mitochondrial damage. These findings suggested that PK11195 may alleviate the damage of LPS-induced cognitive dysfunction of rats by inhibiting microglia activation and autophagy.
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http://dx.doi.org/10.3389/fphar.2020.615543DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7941270PMC
January 2021

1,3-Benzodioxole-based Fibrate Derivatives as Potential Hypolipidemic and hepatoprotective agents.

Bioorg Med Chem Lett 2021 Mar 5:127898. Epub 2021 Mar 5.

Department of Pharmacy, Xi'an Jiaotong University, Xi'an 710061, China. Electronic address:

A series of target compounds 1,3-benzodioxole-based fibrate derivatives were designed and synthesized. All the target compounds were preliminarily evaluated by hyperlipidemia mice induced by Triton WR-1339, in which compound 12 displayed a greater anti-hyperlipidemia activity than other compounds as well as positive drug fenofibrate (FF). 12 showed a significant reduction of plasma lipids, such as triglycerides (TG), total cholesterol (TC) and low-density lipoprotein cholesterin (LDL-C), in high fat diet (HFD) induced hyperlipidemic mice. In addition, hepatic transaminases (AST and ALT) were ameliorated after administration of 12, in particular the AST, and the histopathological examination showed that 12 improved the hepatic lipid accumulation. The expression of PPAR-α involved in lipids metabolism was up-regulated in the liver tissues of 12-treated group. Other significant activity such as antioxidant, and anti-inflammation was confirmed and reinforced the effects of 12 as a potential hypolipidemia and hepatoprotective agent.
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http://dx.doi.org/10.1016/j.bmcl.2021.127898DOI Listing
March 2021

[2,3,5,4'-Tetrahydroxystibane-2-O-β-D-glucoside induces liver injury by disrupting bile acid homeostasis and phospholipids efflux].

Zhongguo Zhong Yao Za Zhi 2021 Jan;46(1):139-145

Jiangsu Key Laboratory of Drug Screening, China Pharmaceutical University Nanjing 210009, China Center for Drug Research and Development, Guangdong Pharmaceutical University Guangzhou 510006, China.

Polygonum multiflorum is a traditional Chinese herbal medicine and has many biological activities such as hair-blacking, anti-atherosclerosis, anti-inflammatory and anti-aging. However, the liver injury induced by P. multiflorum has aroused wide attention in recent years. 2,3,5,4'-tetrahydroxystibane-2-O-β-D-glucoside(TSG) is a main component of P. multiflorum, but the role of TSG in inducing liver injury is unclear. The aim of present study was to evaluate TSG's potential liver injury and effects on bile acid homeostasis and phospholipids efflux. C57 BL/6 J mice received intraperitoneal administration of 400 mg·kg~(-1) of TSG daily for 15 days, and then biochemical indexes of liver injury and changes of phospholipid content were detected. The changes of bile acid compositions were detected by LC-MS/MS. The results showed TSG 400 mg·kg~(-1) significantly increased the content of serum total bile acid(TBA) and alkaline phosphatase(ALP). Elevated free bile acid levels were observed in TSG-treated groups, including β-muricholic acid(β-MCA), ursodeoxycholic acid(UDCA), hyodeoxycholic acid(HDCA), chenodeoxycholic acid(CDCA), deoxcholic acid(DCA) in serum and β-MCA, CDCA in liver. TSG inhibited the protein expression of farnesoid X receptor(FXR) and down stream bile salt export pump(BSEP), which may result in the accumulation of bile acid. TSG also inhibited the expression of 25-hydroxycholesterol-7 alpha-hydroxylase(CYP7 B1), which may disturb the alternative pathway for bile acid synthesis. In addition, intraperitoneal injection of TSG 400 mg·kg~(-1) significantly decreased the content of phospholipids in bile. The research showed that TSG significantly inhibited the expression of multidrug resistance protein 2(MDR2) and destroyed the regular distribution of MDR2 on the bile duct membrane of liver. In vitro results showed that the IC_(50) of TSG on HepG2 cells was about 1 500 μmol·L~(-1) and TSG at 500 μmol·L~(-1)(for 24 h) could destroy the distribution of MDR2 on the bile duct membrane of liver. In conclusion, TSG induced liver injury by disrupting bile acid homeostasis and phospholipids efflux.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20200818.401DOI Listing
January 2021

Precisely Engineered Photoreactive Titanium Nanoarray Coating to Mitigate Biofouling in Ultrafiltration.

ACS Appl Mater Interfaces 2021 Mar 22;13(8):9975-9984. Epub 2021 Feb 22.

Department of Chemical and Environmental Engineering, Yale University, New Haven, Connecticut 06520-8286, United States.

To combat biofouling on membranes, diverse nanostructures of titanium dioxide (TiO) have emerged as effective antimicrobial coatings due to TiO's abilities to transport charge and photoinduce oxidation. However, TiO composite polymeric membranes synthesized using traditional methods of growing crystals have proven chemically unstable, with loss of coating and diminishing antimicrobial performance. Thus, new fabrication methods to enhance durability and efficacy should be considered. In this work, we propose a stepwise approach to construct a stable, uniform TiO nanoarray of regularly spaced, aligned crystals on the surface of a polytetrafluoroethylene ultrafiltration membrane using precisely controlled atomic layer deposition (ALD) followed by solvothermal deposition. We demonstrate that ALD can uniformly seed TiO nanoparticles on the membrane surface with atomic-scale precision. Subsequently, solvothermal deposition assembles and aligns a uniform TiO nanoarray forest. In the presence of sunlight, this TiO nanoarray effectively inactivates any deposited bacteria, increasing flux recovery after membrane cleaning. By systematically investigating this antimicrobial activity, we found that TiO both physically damages cell membranes as well as produces reactive oxygen species in the presence of sunlight that inactivate bacteria. Our study provides an effective bottom-up synthesis scheme to optimize and tailor antifouling TiO coatings for ultrafiltration and other surfaces for a wide range of applications.
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http://dx.doi.org/10.1021/acsami.0c21221DOI Listing
March 2021

Comparisons between self-reported and interview-verified psychotic-like experiences in adolescents.

Early Interv Psychiatry 2021 Feb 16. Epub 2021 Feb 16.

Department of Social Psychiatry, The Affiliated Brain Hospital of Guangzhou Medical University (Guangzhou Huiai Hospital), Guangzhou, China.

Aim: The 15-item positive subscale of the Community Assessment of Psychic Experiences (CAPE-P15) has been widely used for measuring self-reported psychotic-like experiences (PLEs). However, its validity has not been well established. This study aimed to explore the consistency of self-reported PLEs (PLEs-S) and interview-verified PLEs (PLEs-I) based on the same items of the CAPE-P15.

Methods: A total of 1255 college students completed the CAPE-P15 for measuring lifetime and current PLEs. Half of the students with high-risk scores and 5% of the rest were interviewed through telephone. Telephone interviews were based on the items of the CAPE-P15 using the symptom criteria for attenuated positive symptom syndrome.

Results: When considering the presence of PLEs only, all κ values and correspondence rates (CRs) fell below the thresholds. However, there was adequate consistency for lifetime PLEs when associated distress was also considered in self-report (κ = .432, CR = 90.0%). Among three factors, only bizarre experiences (BEs) showed adequate diagnostic accuracy in detecting lifetime PLEs when combined with distress. Cut-off points of 1.30 (sensitivity of 89.2% and specificity of 92.3%) and 1.57 (sensitivity of 79.2% and specificity of 73.8%) for frequency scores were found to best identify genuine PLEs during lifetime and in the past month, respectively.

Conclusions: Although the validity of the CAPE-P15 for genuine PLEs is unsatisfactory, the scale showed much better diagnostic accuracy when combined with associated distress, especially for detecting lifetime PLEs. Self-report items on BEs may be more sensitive and specific when identifying PLEs in late adolescence.
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http://dx.doi.org/10.1111/eip.13132DOI Listing
February 2021

Early therapeutic interventions of traditional Chinese medicine in COVID-19 patients: A retrospective cohort study.

J Integr Med 2021 Jan 13. Epub 2021 Jan 13.

Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China.

Objective: To observe the early interventions of traditional Chinese Medicine (TCM) on the conversion time of nucleic acid in patients with coronavirus disease 2019 (COVID-19), and find possible underlying mechanisms of action.

Methods: A retrospective cohort study was conducted on 300 confirmed COVID-19 patients who were treated with TCM, at a designated hospital in China. The patients were categorized into three groups: TCM1, TCM2 and TCM3, who respectively received TCM interventions within 7, 8-14, and greater than 15 days of hospitalization. Different indicators such as the conversion time of pharyngeal swab nucleic acid, the conversion time of fecal nucleic acid, length of hospital stay, and inflammatory markers (leukocyte count, and lymphocyte count and percentage) were analyzed to observe the impact of early TCM interventions on these groups.

Results: The median conversion times of pharyngeal swab nucleic acid in the three groups were 5.5, 7 and 16 d (P < 0.001), with TCM1 and TCM2 being statistically different from TCM3 (P < 0.01). TCM1 (P < 0.05) and TCM3 (P < 0.01) were statistically different from TCM2. The median conversion times of fecal nucleic acid in the three groups were 7, 9 and 17 d (P < 0.001). Conversion times of fecal nucleic acid in TCM1 were statistically different from TCM3 and TCM2 (P < 0.01). The median lengths of hospital stay in the three groups were 13, 16 and 21 d (P < 0.001). TCM1 and TCM2 were statistically different from TCM3 (P < 0.01); TCM1 and TCM3 were statistically different from TCM2 (P < 0.01). Both leucocyte and lymphocyte counts increased gradually with an increase in the length of hospital stay in TCM1 group patients, with a statistically significant difference observed at each time point in the group (P < 0.001). Statistically significant differences in lymphocyte count and percentage in TCM2 (P < 0.001), and in leucocyte count (P = 0.043) and lymphocyte count (P = 0.038) in TCM3 were observed. The comparison among the three groups showed a statistically significant difference in lymphocyte percentage on the third day of admission (P = 0.044).

Conclusion: In this study, it was observed that in COVID-19 patients treated with a combination of Chinese and Western medicines, TCM intervention earlier in the hospital stay correlated with faster conversion time of pharyngeal swab and fecal nucleic acid, as well as shorter length of hospital stay, thus helping promote faster recovery of the patient. The underlying mechanism of action may be related to improving inflammation in patients with COVID-19.
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http://dx.doi.org/10.1016/j.joim.2021.01.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7832636PMC
January 2021

High-Performance, Free-Standing Symmetric Hybrid Membranes for Osmotic Separation.

ACS Appl Mater Interfaces 2021 Feb 12;13(7):8967-8975. Epub 2021 Feb 12.

College of Environment, Research Centre for Municipal Wastewater Treatment and Water Quality Protection, Northeast Normal University, Changchun 130117, China.

The internal concentration polarization (ICP) of asymmetric osmotic membranes with support layers greatly reduced membrane water permeability, therefore compromising membrane performance. In this study, a series of free-standing symmetric hybrid forward osmosis (FO) membranes without experiencing ICP were fabricated by covalently linking metal-organic framework (MOF) nanofillers with a polymer matrix. Owing to the introduction of MOFs, which allow only water permeation but reject salts by steric hindrance, the prepared hybrid membranes could approach the empirical permeability-selectivity trade-off. The optimized hybrid membrane displayed an outstanding water/NaSO selectivity of ∼1208.4 L mol, compared with that of conventional membranes of ∼375.6 L mol. Additionally, the fabricated hybrid membranes showed excellent mechanical robustness, maintaining structural integrity during the long-term FO separation of high-salinity solution. This work provides an effective methodology to fabricate high-performance, symmetric MOF-based membranes for osmotic separation processes such as seawater desalination and water purification.
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http://dx.doi.org/10.1021/acsami.0c22124DOI Listing
February 2021

Association between solid fuel use and cognitive decline among middle-aged and elderly Chinese adults: a longitudinal study.

Sci Rep 2021 Feb 11;11(1):3634. Epub 2021 Feb 11.

School of Public Health, Dalian Medical University, 9 Western Section, Lvshun South Street, Lvshunkou District, Dalian, People's Republic of China.

This study was conducted to investigate (1) the association between solid fuel use for cooking and cognitive function; (2) the moderating effects of gender and residential area on cognitive scores among solid fuel users; and (3) the effects of solid fuel use on cognitive decline among different gender and age subgroups in 2011-2018. A total of 5140 Chinese middle-aged and elderly participants were successfully followed for 7 years (2011-2018). Solid fuel use was self-reported as using solid fuel for cooking at home, and cognitive function was assessed by 4 parts: episodic memory, time orientation, numerical ability and picture drawing. After adjusting for covariates, solid fuel users had lower cognitive scores, and the moderation effects of gender and residence on cognitive function were significant among the solid fuel users. In addition, compared with the group of clean fuel users, solid fuel users had a faster decline rate of cognitive function among the subgroups of female and elderly people.
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http://dx.doi.org/10.1038/s41598-021-83171-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7878745PMC
February 2021

Self-assembled luminescent cholate gels induced by a europium ion in deep eutectic solvents.

Soft Matter 2021 Mar;17(10):2815-2822

Key Laboratory of Colloid and Interface Chemistry, Shandong University, Ministry of Education, Jinan, 250100, China.

Deep eutectic solvents (DESs) with excellent physicochemical properties similar to ionic liquids and biocompatibility are potential solvent candidates for designing novel lanthanide luminescent soft materials. In this paper, the fabrication and characterization of such luminescent gels in three choline chloride (ChCl)-based DESs through self-assembly of the sodium cholate and europium nitrate are presented. The microstructure and gel-like nature of the obtained eutectogels were explored and confirmed by scanning electron microscopy and rheology measurements. While Fourier transform infrared spectroscopy and small-angle X-ray scattering were used to analyze the gel formation mechanism, which was considered to be synergistically driven by metal coordination, hydrogen bonding and solvophobic interactions. All three eutectogels exhibited good photophysical properties. Among these, the one formed in ChCl/urea DES was found to possess the strongest mechanical strength. While the one formed in ChCl/glycerol DES exhibited the longest luminescence lifetime and quantum efficiency. The obtained results demonstrate the possibility of using DESs to construct lanthanide luminescent soft materials or control their properties through the choice of hydrogen-bond donor molecules.
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http://dx.doi.org/10.1039/d0sm02224aDOI Listing
March 2021

WeChat as a Platform for Baduanjin Intervention in Patients With Stable Chronic Obstructive Pulmonary Disease in China: Retrospective Randomized Controlled Trial.

JMIR Mhealth Uhealth 2021 Feb 2;9(2):e23548. Epub 2021 Feb 2.

Department of Respiratory Medicine, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.

Background: Pulmonary rehabilitation is a crucial part of the nonpharmacological treatment of stable chronic obstructive pulmonary disease (COPD), but management remains problematic. WeChat could serve as a useful tool in patient management. Baduanjin is a popular exercise in China that is usually applied in pulmonary rehabilitation, which has been confirmed to be effective in improving lung function and life quality.

Objective: This study aimed to explore the efficiency of WeChat in the management of Baduanjin exercise in COPD patients.

Methods: A total of 200 patients from the respiratory department of Putuo Hospital participated in the Baduanjin rehabilitation project from September 2018 to October 2019, and were randomly assigned to the WeChat and control groups and followed up using the WeChat platform or telephone for 12 weeks. The frequency of Baduanjin exercise, lung function (percentage of forced expiratory volume in 1 second predicted, FEV1% predicted), and COPD assessment test (CAT) scores were collected and compared between the two groups. The number of message exchanges and a satisfaction survey on the WeChat platform were used to assess the feasibility of WeChat management outside the hospital.

Results: The Baduanjin exercise frequency significantly differed between the control group and WeChat group (F=33.82, P<.001) and across various time points (F=214.87, P<.001). After the follow-up on WeChat, there were fewer patients not performing Baduanjin exercise. The FEV1% predicted value significantly differed before and after Baduanjin exercise in the control group (Z=-3.686, P<.001) and the WeChat group (Z=-6.985, P<.001). A significant difference in the FEV1% predicted value was observed after Baduanjin exercise between the two groups (Z=-3.679, P<.001). The CAT score significantly differed before and after Baduanjin exercise in the control group (Z=-4.937, P<.001) and the WeChat group (Z=-5.246, P<.001). A significant difference in the CAT score was observed after Baduanjin exercise between the two groups (Z=-5.246, P<.001). The number of completed Baduanjin exercises, lung function, and CAT scores in active patients were higher than those in nonactive patients. All satisfaction survey items were scored with more than 4 points. Among the items, the highest score (mean 4.54, SD 0.77) was for continued WeChat management, followed by the effective management of Baduanjin exercise (mean 4.46, SD 0.87). The patients in the WeChat group showed much higher enthusiasm for and compliance with Baduanjin exercise, resulting in better life quality and lung function. The patients were very satisfied with the WeChat management because of the obvious curative effect and home feeling.

Conclusions: The WeChat platform provided a feasible, effective, and sustainable management plan for Baduanjin rehabilitation.

Trial Registration: Chinese Clinical Trial Registry ChiCTR1900028248; http://www.chictr.org.cn/showprojen.aspx?proj=46995.
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http://dx.doi.org/10.2196/23548DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7886617PMC
February 2021

circRNA_0005529 facilitates growth and metastasis of gastric cancer via regulating miR-527/Sp1 axis.

BMC Mol Cell Biol 2021 Jan 20;22(1). Epub 2021 Jan 20.

Department of Emergency, Huai'an Hospital Affiliated of Xuzhou Medical University and Huai'an Second People's Hospital, No. 62 Huaihai South Road, Huai 'an City, Jiangsu Province, China.

Background: Circular RNAs (circRNAs) are endogenous non-coding RNAs, which are associated with various biological processes, including microRNA (miRNA) interaction, protein binding and regulatory splicing. circRNA_0005529 (circ_0005529) is derived from vacuolar protein sorting 33 homologue B (VPS33B), and its biological role in gastric cancer (GC) has not been examined. In this study, the expression and location of circ_0005529 and microRNA-527 (miR-527) were determined by qRT-PCR and fluorescence in situ hybridization (FISH). Cell proliferation and cell migration were determined by MTT, EdU incorporation, colony formation, wound scratch and transwell assays. In addition, immunohistochemistry and western blotting were performed to determine the expressions of specificity protein 1 (Sp1), PCNA, c-myc, E-cadherin and N-cadherin. Western blotting and luciferase reporter assay were performed to study the interaction between circ_0005529 and miR-527 or miR-527 and Sp1. The functional effects of circ_0005529 on GC through regulating Sp1 were further evaluated using xenograft and metastatic mouse models in vivo.

Results: Our results showed that circ_0005529 was upregulated in GC tissues and cells, and had promoting effects on cell proliferation and cell migration. Mechanism analysis suggested that circ_0005529 could bind to microRNA-527 (miR-527) and reduce its expression. The interaction between miR-527 and Sp1 in GC was systematically studied. In addition, the results indicated that Sp1 upregulation could rescue the effects on cell proliferation and migration caused by circ_0005529. Moreover, the inhibitory effects of circ_0005529 downregulation on GC growth and metastasis were evaluated in mouse models. These findings suggested that the axis of circ_0005529/miR-527/Sp1 may serve as a promising treatment target for GC diagnosis and treatment.

Conclusions: These findings suggested that the signal axis of circ_0005529/miR-527/Sp1 may has the potential to be explored as a novel therapeutic target for GC diagnosis and treatment. Mechanism diagram: During GC development, overexpressed circ_0005529 sponged miR-527 and then upregulated the expression of Sp1. Subsequently, epithelial-mesenchymal transition (EMT), cell proliferation and cell migration were promoted, which ultimately facilitated the tumor metastasis.
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http://dx.doi.org/10.1186/s12860-020-00340-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7816457PMC
January 2021

Non-invasive evaluation of labial gingival and alveolar crest thickness in the maxillary anterior teeth region by 15-MHz B-mode ultrasonography.

BMC Oral Health 2021 Jan 6;21(1):10. Epub 2021 Jan 6.

Department of Stomatology, Shenzhen University General Hospital and Shenzhen University Clinical Medical Academy, Liuxian Street, Nanshan district, Shenzhen, 518055, Guangdong, China.

Background: Knowledge of gingival thickness (GT) and alveolar crest thickness (ACT) is essential when performing surgical and non-surgical procedures in the maxillary anterior teeth region. This study aimed at evaluating the GT and ACT in the maxillary anterior teeth region using 15-MHz B-mode Ultrasonic (US).

Methods: A total of 300 teeth from 50 healthy participants, comprising 25 women and 25 men, aged between 18 and 35 years were analyzed. We measured labial periodontal tissue structures of maxillary anterior teeth, including GT and ACT, at 3 mm apical to the gingival margin (GT3) and the crestal level, respectively. The GT and ACT measurements were correlated.

Results: The mean labial GT3 of the maxillary central incisors, lateral incisors, and canines were 1.24 ± 0.03 mm, 1.21 ± 0.03 mm and 1.11 ± 0.03 mm, respectively. Canine GT3 was significantly thin than those in the central and lateral incisors (P < 0.05). With regards to labial ACT, we recorded 0.79 ± 0.03 mm, 0.76 ± 0.02 mm and 0.73 ± 0.02 mm for maxillary central incisors, lateral incisors and canines, respectively. There were no significant differences in ACT of maxillary anterior teeth (P > 0.05). GT3 of men was greater than that of women (P < 0.05). In addition, GT and ACT were positively correlated (r = 0.32, P < 0.01).

Conclusion: 15-MHz B-mode US is an effective tool for measuring labial GT and ACT of anterior teeth. There are sex-associated differences in GT3 and the correlation between the GT3 and ACT of anterior teeth is moderately positive.
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http://dx.doi.org/10.1186/s12903-020-01377-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7789343PMC
January 2021

The roles of liver X receptor α in inflammation and inflammation-associated diseases.

J Cell Physiol 2021 Jul 10;236(7):4807-4828. Epub 2020 Dec 10.

Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education Life of Sciences, Northwest University, Xi'an, China.

Liver X receptor α (LXRα; also known as NR1H3), an isoform of LXRs, is a member of the nuclear receptor family of transcription factors and plays essential roles in the transcriptional control of cholesterol homeostasis. Previous in-depth phenotypic analyses of mouse models with deficient LXRα have also demonstrated various physiological functions of this receptor within inflammatory responses. LXRα activation exerts a combination of metabolic and anti-inflammatory actions resulting in the modulation and the amelioration of inflammatory disorders. The tight "repercussions" between LXRα and inflammation, as well as cholesterol homeostasis, have suggested that LXRα could be pharmacologically targeted in pathologies such as atherosclerosis, acute lung injury, and Alzheimer's disease. This review gives an overview of the recent advances in understanding the roles of LXRα in inflammation and inflammation-associated diseases, which will help in the design of future experimental researches on the potential of LXRα and advance the investigation of LXRα as pharmacological inflammatory targets.
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http://dx.doi.org/10.1002/jcp.30204DOI Listing
July 2021

Proposal of a TNM classification-based staging system for esthesioneuroblastoma: More precise prediction of prognosis.

Head Neck 2021 Apr 10;43(4):1097-1104. Epub 2020 Dec 10.

Department of Radiation Oncology, National Cancer Center/ National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Background: Esthesioneuroblastoma (ENB) is a rare malignant neoplasm. Currently, no consistent and universal staging system for ENB exists. The aim of this study is to propose a TNM-based classification.

Subjects And Methods: Hundred and forty-two patients from our institution, with ENB pathologically confirmed between July 1978 and December 2018, were reviewed. All patients were restaged according to the Kadish stage, Morita stage and American Joint Committee on Cancer (AJCC) T classification from clinical and radiological data. Multivariate Cox proportional hazard regression analyses were performed to determine the impact of various factors. The goodness-of-fit and predictive accuracy of the different staging systems were calculated using R software.

Results: The median follow-up time was 57 months (range: 4-229 months). According to the Kadish system, the 5-year overall survival (OS) for patients with stage A, B and C was 100%, 83.6% and 64.2%, respectively (P = .055). With respect to the Morita classification, 5-year OS for stages A, B, C and D was 100%, 83.6%, 70.7% and 50.0%, respectively (P = .004). Analysis based on the proposed staging model demonstrated 5-year OS for stage I, II, III and IV disease was 100%, 88.9%, 75.9% and 49.0%, respectively (P < .001). In separate multivariate Cox regression models, only the novel staging system exhibited independent effects on OS (P = .004); the Akaike information criterion and Harrell's concordance index were also superior to those calculated for the Kadish or Morita systems.

Conclusions: The proposed TNM-based staging system offers an improved prognostic assessment for patients with ENB. Further verification and refinement from additional dataset application is required.
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http://dx.doi.org/10.1002/hed.26559DOI Listing
April 2021

Photo-electrochemical Osmotic System Enables Simultaneous Metal Recovery and Electricity Generation from Wastewater.

Environ Sci Technol 2021 01 8;55(1):604-613. Epub 2020 Dec 8.

Department of Chemical and Environmental Engineering, Yale University, New Haven, Connecticut 06520-8286, United States.

Global depletion of natural resources provides an impetus for developing low-cost, environmentally benign technologies for the recovery of valuable resources from wastewater. In this study, we present an autonomous photo-electrochemical osmotic system (PECOS) that can recover a wide range of metals from simulated metal-laden wastewater with sunlight illumination while generating electricity. The PECOS comprises a draw solution chamber with a nickel nanoparticle-functionalized titanium nanowire (Ni-TiNA) photoanode, a feed solution chamber containing synthetic wastewater with an immersed carbon fiber cathode, and a forward osmosis (FO) membrane mounted between the chambers as a separator. Using a Na-EDTA anolyte as a draw solution at neutral pH, we demonstrate that a sunlit PECOS achieves copper recovery at a rate of 51 g h per m of membrane area from simulated copper-laden wastewater while simultaneously producing a maximum power density of 228 mW m. Moreover, because of the osmotic pressure difference generated by the photo-electrochemical reactions, the PECOS reduces the wastewater volume by extracting fresh water through the FO membrane at a water flux of 0.84 L m h. We further demonstrate the feasibility of the PECOS in recovering diverse metals from a simulated metal-laden industrial wastewater under sunlight irradiation. Our proof-of-concept PECOS prototype provides a sustainable technological solution that leverages sunlight in an electrochemical osmotic system to recover multiple resources from wastewater.
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http://dx.doi.org/10.1021/acs.est.0c04375DOI Listing
January 2021

Janus electrocatalytic flow-through membrane enables highly selective singlet oxygen production.

Nat Commun 2020 12 4;11(1):6228. Epub 2020 Dec 4.

Department of Chemical and Environmental Engineering, Yale University, New Haven, CT, 06520-8286, USA.

The importance of singlet oxygen (O) in the environmental and biomedical fields has motivated research for effective O production. Electrocatalytic processes hold great potential for highly-automated and scalable O synthesis, but they are energy- and chemical-intensive. Herein, we present a Janus electrocatalytic membrane realizing ultra-efficient O production (6.9 mmol per m of permeate) and very low energy consumption (13.3 Wh per m of permeate) via a fast, flow-through electro-filtration process without the addition of chemical precursors. We confirm that a superoxide-mediated chain reaction, initiated by electrocatalytic oxygen reduction on the cathodic membrane side and subsequently terminated by HO oxidation on the anodic membrane side, is crucial for O generation. We further demonstrate that the high O production efficiency is mainly attributable to the enhanced mass and charge transfer imparted by nano- and micro-confinement effects within the porous membrane structure. Our findings highlight a new electro-filtration strategy and an innovative reactive membrane design for synthesizing O for a broad range of potential applications including environmental remediation.
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http://dx.doi.org/10.1038/s41467-020-20071-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7718259PMC
December 2020

Sigma-2 Receptor-A Potential Target for Cancer/Alzheimer's Disease Treatment via Its Regulation of Cholesterol Homeostasis.

Molecules 2020 Nov 20;25(22). Epub 2020 Nov 20.

School of Chemistry, Chemical Engineering and Life Science, Wuhan University of Technology, 122 Luoshi Road, Wuhan 430070, China.

The sigma receptors were classified into sigma-1 and sigma-2 receptor based on their different pharmacological profiles. In the past two decades, our understanding of the biological and pharmacological properties of the sigma-1 receptor is increasing; however, little is known about the sigma-2 receptor. Recently, the molecular identity of the sigma-2 receptor has been identified as TMEM97. Although more and more evidence has showed that sigma-2 ligands have the ability to treat cancer and Alzheimer's disease (AD), the mechanisms connecting these two diseases are unknown. Data obtained over the past few years from human and animal models indicate that cholesterol homeostasis is altered in AD and cancer, underscoring the importance of cholesterol homeostasis in AD and cancer. In this review, based on accumulated evidence, we proposed that the beneficial roles of sigma-2 ligands in cancer and AD might be mediated by their regulation of cholesterol homeostasis.
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http://dx.doi.org/10.3390/molecules25225439DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7699687PMC
November 2020

Recent advances in screening active components from natural products based on bioaffinity techniques.

Acta Pharm Sin B 2020 Oct 3;10(10):1800-1813. Epub 2020 Jun 3.

School of Pharmacy, Health Science Center, Xi'an Jiaotong University, Xi'an 710061, China.

Natural products have provided numerous lead compounds for drug discovery. However, the traditional analytical methods cannot detect most of these active components, especially at their usual low concentrations, from complex natural products. Herein, we reviewed the recent technological advances (2015-2019) related to the separation and screening bioactive components from natural resources, especially the emerging screening methods based on the bioaffinity techniques, including biological chromatography, affinity electrophoresis, affinity mass spectroscopy, and the latest magnetic and optical methods. These screening methods are uniquely advanced compared to other traditional methods, and they can fish out the active components from complex natural products because of the affinity between target and components, without tedious separation works. Therefore, these new tools can reduce the time and cost of the drug discovery process and accelerate the development of more effective and better-targeted therapeutic agents.
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http://dx.doi.org/10.1016/j.apsb.2020.04.016DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7606101PMC
October 2020

Further insights into the phylogeny of peniculid ciliates (Ciliophora, Oligohymenophorea) based on multigene data.

Mol Phylogenet Evol 2021 01 1;154:107003. Epub 2020 Nov 1.

Key Laboratory of Biodiversity of Aquatic Organisms, Harbin Normal University, Harbin 150025, PR China. Electronic address:

Peniculids comprise a large order of ciliated protists in Class Oligohymenophorea having many unresolved evolutionary relationships. Herein, we report 27 new sequences, including 18S rRNA, ITS1-5.8S- ITS2 rRNA, 28S rRNA and the mitochondrial cox1 genes of eight peniculids. We conducted phylogenetic analyses based on each these markers and on a four-gene concatenated data set (18S rRNA, ITS1-5.8S- ITS2 rRNA, 28S rRNA, and cox1 gene). The main findings are: 1) subclass Peniculia and family Parameciidae are monophyletic, with genus Frontonia remaining non-monophyletic; 2) Urocentrids have traditionally been regarded as a family, multi-gene analyses support the rank of Urocentrida and consistently recovers this order as sister to Peniculida, and Urocentrida and Peniculida comprise subclass Peniculia in agreement with Lynn's (2008) classification; 3) discrepancies between multiple-gene phylogenies, and conflicts with morphologic data regarding genus Frontonia necessitate expansion and revision of species diagnoses and we propose consideration of Group III of Frontonia (including F. didieri, F. ocularis, F. anatolica, F. pusilla and F. elegans) as incertae sedis in Peniculida; 4) multi-gene analyses of Parameciidae support five previously established subgenera. Paramecium buetschlii is placed in subgenus Chloroparamecium, and P. chlorelligerum into subgenus Viridoparamecium.
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http://dx.doi.org/10.1016/j.ympev.2020.107003DOI Listing
January 2021

Multifunctional Nanoparticles Boost Cancer Immunotherapy Based on Modulating the Immunosuppressive Tumor Microenvironment.

ACS Appl Mater Interfaces 2020 Nov 30;12(45):50734-50747. Epub 2020 Oct 30.

Department of Pharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, Liaoning 110016, P. R. China.

Cancer immunotherapy has been a favorable strategy for facilitating antitumor immunity. However, immune tolerance and an ultimate immunosuppressive tumor microenvironment (ITM) are primary obstacles. To achieve the goals of remodeling the ITM and promoting cancer immunotherapy, a versatile nanoparticle codelivering shikonin (SK) and PD-L1 knockdown siRNA (SK/siR-NPs) was reported. SK/siR-NPs are demonstrated to tellingly induce the immunogenic cell death (ICD) of tumor cells, leading to increased dendritic cell maturation. Moreover, SK/siR-NPs can cause an efficacious inhibition of PD-L1, leading to enhanced cytotoxic T lymphocyte response to tumor cells. Most importantly, SK/siR-NPs can restrain lactate production via the downregulation of pyruvate kinase-M2 (PKM2) and eventually repolarize tumor associated macrophages (TAMs) from the M2-subtype to M1-subtype states. Meanwhile, SK/siR-NPs suppress regulatory T lymphocytes to fight with the ITM. Overall, the developed co-delivery system presents a significant potential for cancer immunotherapy through simultaneously inducing ICD, repolarizing M2-TAMs, and relieving PD-L1 pathway-regulated immune tolerance.
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http://dx.doi.org/10.1021/acsami.0c14909DOI Listing
November 2020

High-intensity focused ultrasound ablation combined with transcatheter arterial chemoembolization improves long-term efficacy and prognosis of primary liver cancer.

J Clin Lab Anal 2021 Feb 25;35(2):e23633. Epub 2020 Oct 25.

Interventional Vascular Surgery, Affiliated Hospital of Hebei University, Baoding, Hebei, China.

Background: To investigate the clinical efficacy of high-intensity focused ultrasound (HIFU) combined with transcatheter arterial chemoembolization (TACE) in the treatment of primary liver cancer (PLC) and its effect on the prognosis of patients.

Methods: A total of 132 patients with PLC admitted to our hospital were selected for the study, among whom 68 patients received TACE combined with HIUF and were assigned to the observation group (OG), whereas the remaining 54 patients were treated with TACE alone and were assigned to the control group (CG). The factors influencing the patients' prognosis were also evaluated by multivariate analysis.

Results: The total effective rate of the OG was 83.82%, which was significantly higher than that of 55.56% of the CG (P < .05). No significant difference was found in incidence of adverse reactions between the two groups (P > .05). After treatment, the increases of CD3+, CD4+, CD4+/CD8+, and NK cells in the OG were more significant than those in the CG (P < .05). However, the decrease of CD8+ cells was more significant in the OG than that in the CG (P < .05). The 3-year survival rate of patients in the OG was 61.76%, which was significantly higher than that of 40.74% in the CG (P < .05).

Conclusion: The application of TACE combined with HIFU is effective in treating PLC, which can prolong the life expectancy and improve the prognosis of patients with PLC without increasing the incidence of adverse reactions.
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http://dx.doi.org/10.1002/jcla.23633DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7891537PMC
February 2021

Exogenous L-arginine increases intestinal stem cell function through CD90+ stromal cells producing mTORC1-induced Wnt2b.

Commun Biol 2020 Oct 23;3(1):611. Epub 2020 Oct 23.

State Key Laboratory of Animal Nutrition, Department of Animal Nutrition & Feed Science, College of Animal Science & Technology, China Agricultural University, Haidian District, Beijing, 100193, China.

The renewal and repair of intestinal epithelium depend on the self-renewal of intestinal stem cells (ISCs) under physiological and pathological conditions. Although previous work has established that exogenous nutrients regulate adult stem cell activity, little is known about the regulatory effect of L-arginine on ISCs. In this study we utilize mice and small intestinal (SI) organoid models to clarify the role of L-arginine on epithelial differentiation of ISCs. We show that L-arginine increases expansion of ISCs in mice. Furthermore, CD90 intestinal stromal cells augment stem-cell function in response to L-arginine in co-culture experiments. Mechanistically, we find that L-arginine stimulates Wnt2b secretion by CD90 stromal cells through the mammalian target of rapamycin complex 1 (mTORC1) and that blocking Wnt2b production prevents L-arginine-induced ISC expansion. Finally, we show that L-arginine treatment protects the gut in response to injury. Our findings highlight an important role for CD90+ stromal cells in L-arginine-stimulated ISC expansion.
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http://dx.doi.org/10.1038/s42003-020-01347-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7584578PMC
October 2020

Effects of Tanreqing Capsule on the negative conversion time of nucleic acid in patients with COVID-19: A retrospective cohort study.

J Integr Med 2021 01 3;19(1):36-41. Epub 2020 Oct 3.

Department of Respiratory Disease, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. Electronic address:

Objective: Traditional Chinese medicine plays a significant role in the treatment of the pandemic of coronavirus disease 2019 (COVID-19). Tanreqing Capsule (TRQC) was used in the treatment of COVID-19 patients in the Shanghai Public Health Clinical Center. This study aimed to investigate the clinical efficacy of TRQC in the treatment of COVID-19.

Methods: A retrospective cohort study was conducted on 82 patients who had laboratory-confirmed mild and moderate COVID-19; patients were treated with TRQC in one designated hospital. The treatment and control groups consisted of 25 and 57 cases, respectively. The treatment group was given TRQC orally three times a day, three pills each time, in addition to conventional Western medicine treatments which were also administered to the control group. The clinical efficacy indicators, such as the negative conversion time of pharyngeal swab nucleic acid, the negative conversion time of fecal nucleic acid, the duration of negative conversion of pharyngeal-fecal nucleic acid, and the improvement in the level of immune indicators such as T-cell subsets (CD3, CD4 and CD45) were monitored.

Results: COVID-19 patients in the treatment group, compared to the control group, had a shorter negative conversion time of fecal nucleic acid (4 vs. 9 days, P = 0.047) and a shorter interval of negative conversion of pharyngeal-fecal nucleic acid (0 vs. 2 days, P = 0.042). The level of CD3 T cells increased in the treatment group compared to the control group ([317.09 ± 274.39] vs. [175.02 ± 239.95] counts/μL, P = 0.030). No statistically significant differences were detected in the median improvement in levels of CD4 T cells (173 vs. 107 counts/μL, P = 0.208) and CD45 T cells (366 vs. 141 counts/μL, P = 0.117) between the treatment and control groups.

Conclusion: Significant reductions in the negative conversion time of fecal nucleic acid and the duration of negative conversion of pharyngeal-fecal nucleic acid were identified in the treatment group as compared to the control group, illustrating the potential therapeutic benefits of using TRQC as a complement to conventional medicine in patients with mild and moderate COVID-19. The underlying mechanism may be related to the improved levels of the immune indicator CD3 T cells.
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http://dx.doi.org/10.1016/j.joim.2020.10.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7532751PMC
January 2021