Publications by authors named "Meng Shen"

80 Publications

Flavonoids from Whole-Grain Oat Alleviated High-Fat Diet-Induced Hyperlipidemia Regulating Bile Acid Metabolism and Gut Microbiota in Mice.

J Agric Food Chem 2021 Jul 2;69(27):7629-7640. Epub 2021 Jul 2.

School of Medical Instruments and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China.

A high-fat diet (HFD) causes hyperlipidemia, which worsens disturbances in bile acid (BA) metabolism and gut microbiota. This study aimed to investigate the regulation of flavonoids from whole-grain oat (FO) on BA metabolism and gut microbiota in HFD-induced hyperlipidemic mice. The experiment results showed that FO improved serum lipid profiles and decreased body weight and lipid deposition in HFD-fed mice. Through real-time qualitative polymerase chain reaction (RT-qPCR) and Western blot assays, by up-regulating the expression of PPARα, CPT-1, CYP7A1, FXR, TGR5, NTCP, and BSTP, and down-regulating those of SREBP-1c, FAS, and ASBT, FO suppressed lipogenesis, promoted lipolysis and BA synthesis, and efflux to faeces the FXR pathway. 16s rRNA sequencing revealed that FO significantly increased and significantly decreased , , , and . Spearman's correlation analysis showed that these bacteria were strongly correlated with hyperlipidemia-related parameters. Therefore, our results indicated that FO possessed an antihyperlipidemic effect regulating the gut-liver axis, i.e., BA metabolism and gut microbiota.
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http://dx.doi.org/10.1021/acs.jafc.1c01813DOI Listing
July 2021

Investigation of the Mechanism of Complement System in Diabetic Nephropathy via Bioinformatics Analysis.

J Diabetes Res 2021 24;2021:5546199. Epub 2021 May 24.

Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, 610072 Sichuan, China.

Objectives: Diabetic nephropathy (DN) is a major cause of end-stage renal disease (ESRD) throughout the world, and the identification of novel biomarkers via bioinformatics analysis could provide research foundation for future experimental verification and large-group cohort in DN models and patients.

Methods: GSE30528, GSE47183, and GSE104948 were downloaded from Gene Expression Omnibus (GEO) database to find differentially expressed genes (DEGs). The difference of gene expression between normal renal tissues and DN renal tissues was firstly screened by GEO2R. Then, the protein-protein interactions (PPIs) of DEGs were performed by STRING database, the result was integrated and visualized via applying Cytoscape software, and the hub genes in this PPI network were selected by MCODE and topological analysis. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were carried out to determine the molecular mechanisms of DEGs involved in the progression of DN. Finally, the Nephroseq v5 online platform was used to explore the correlation between hub genes and clinical features of DN.

Results: There were 64 DEGs, and 32 hub genes were identified, enriched pathways of hub genes involved in several functions and expression pathways, such as complement binding, extracellular matrix structural constituent, complement cascade related pathways, and ECM proteoglycans. The correlation analysis and subgroup analysis of 7 complement cascade-related hub genes and the clinical characteristics of DN showed that C1QA, C1QB, C3, CFB, ITGB2, VSIG4, and CLU may participate in the development of DN.

Conclusions: We confirmed that the complement cascade-related hub genes may be the novel biomarkers for DN early diagnosis and targeted treatment.
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http://dx.doi.org/10.1155/2021/5546199DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8169258PMC
May 2021

Metastasis-associated gene 1 (MTA1) enhances cisplatin resistance of malignant pleural mesothelioma by ATR-Chk1-mediated DNA repair.

Ann Transl Med 2021 Apr;9(8):670

Department of Oncology of the First Affiliated Hospital of Soochow University, Suzhou, China.

Background: Malignant pleural mesothelioma (MPM) chemoresistance remains a challenge to oncologists. In our previous study, we demonstrated that the aberrant expression of metastasis-associated gene 1 () is associated with carcinogenesis and metastasis in MPM. The aim of the present study was to investigate the mechanism of and chemo-resistance in MPM.

Methods: Western blotting and real-time polymerase chain reaction were used to analyze the protein and mRNA levels. A stable clone with a knockdown of was generated with shRNA via lentivirus technology in MPM cell lines. Cell Counting Kit-8 assay and crystal violet assay were used to measure cell viability. Immunochemical staining was employed to detect expression in MPM tissues. The cell cycle of MPM cells was determined by phosphohistone H3 staining and flow cytometric analysis.

Results: The protein was upregulated and enhanced cisplatin resistance in MPM. Cisplatin stabilized the expression of the protein by inhibiting its ubiquitination, and enhanced G2/M cell cycle delay and regulated and protected the tumor genome from chemotherapeutic drugs via participating in the phosphorylation of the ataxia telangiectasia mutated and rad3 related-checkpoint kinase 1 () pathway.

Conclusions: These data suggest that enhances cisplatin resistance by -mediated DNA damage repairment and cisplatin stabilizes expression via affecting on the ubiquitination pathway of in MPM. Our findings indicate that could serve as a novel therapeutic target to overcome chemoresistance in MPM.
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http://dx.doi.org/10.21037/atm-21-941DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8106096PMC
April 2021

Myeloid-derived suppressor cells regulate the immunosuppressive functions of PD-1PD-L1 Bregs through PD-L1/PI3K/AKT/NF-κB axis in breast cancer.

Cell Death Dis 2021 May 9;12(5):465. Epub 2021 May 9.

Department of Immunology, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Key Laboratory of Cancer Immunology and Biotherapy, Tianjin's Clinical Research Center for Cancer, Tianjin, 300060, China.

Myeloid-derived suppressor cells (MDSCs) are a heterogeneous group of myeloid cells that are closely related to tumor immune escape, but the mechanism by which MDSCs regulate B cells has not been elucidated. Our previous studies revealed that breast cancer-derived MDSCs could induce a group of PD-1PD-L1 Bregs with immunosuppressive functions. Here, we reported that blocking PD-1/PD-L1 interaction between MDSCs and B cells could reverse the immunosuppressive functions of PD-1PD-L1 Bregs. The activation of PI3K/AKT/NF-κB signaling pathway is essential for PD-1PD-L1 Bregs to exert immunosuppressive effects. MDSCs activated the PI3K/AKT/NF-κB pathway in B cells via the PD-1/PD-L1 axis. Furthermore, inhibition of PD-1/PD-L1 or PI3K/AKT signaling suppressed both tumor growth and the immunosuppressive functions of PD-1PD-L1 Bregs. Dual suppression of PD-1/PD-L1 and PI3K/AKT exerted better antitumor effect. Finally, MDSCs and PD-1PD-L1 Bregs were colocalized in breast cancer tissues and PD-1PD-L1 Bregs were positively correlated with poor prognosis. Thus, MDSC-educated PD-1PD-L1 Bregs and their regulatory mechanisms could contribute to the immunosuppressive tumor microenvironment. Our study proposes a novel mechanism for MDSC-mediated regulation of B cell immunity, which might shed new light on tumor immunotherapy.
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http://dx.doi.org/10.1038/s41419-021-03745-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107179PMC
May 2021

Defect Engineering of Photocatalysts towards Elevated CO Reduction Performance.

ChemSusChem 2021 Jul 11;14(13):2635-2654. Epub 2021 May 11.

The State Key Lab of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai, 200050, P. R. China.

Photocatalytic CO reduction provides a promising solution to address the crises of massive CO emissions and fossil energy shortages. As one of the most effective strategies to promote CO photoconversion, defect engineering shows great potential in modulating the electronic structure and light absorption properties of photocatalysts while increasing surface active sites for CO activation and conversion. This Review summarizes the recent progress in defect engineering of photocatalysts to promote CO reduction performances from the following four aspects: 1) Approaches to defect (mainly vacancy and dopant) generation in photocatalysts; 2) defect structure characterization techniques; 3) physical and chemical properties of defect-engineered photocatalysts; 4) CO reduction performance enhancements in activity, selectivity, and stability of photocatalysts by defect engineering. This Review is expected to present readers with a comprehensive view of progress in the field of photocatalytic CO reduction through defect engineering for elevated CO -to-fuels conversion efficiency.
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http://dx.doi.org/10.1002/cssc.202100677DOI Listing
July 2021

Bi-Sn Oxides for Highly Selective CO Electroreduction to Formate in a Wide Potential Window.

ChemSusChem 2021 May 14;14(10):2247-2254. Epub 2021 Apr 14.

State Key Laboratory of High Performance Ceramics and Superfine Microstructure Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai, 200050, P. R. China.

The electroreduction of CO into the highly value-added fuel formic acid (HCOOH) has been considered an ideal approach to convert renewable energy and mitigate environmental crisis. SnO electrode is one of the promising candidates to electrocatalytically convert CO to HCOOH, but its poor stability limits its future development and application. In this study, highly stable SnO /Bi O oxide catalysts are obtained by distributing SnO nanoparticles on the surface of Bi O sheets. The XPS spectra revealed an interfacial electronic transportation from Bi O sheets to SnO nanoparticles, which made SnO rich of electrons. The strong interfacial interaction protected the active sites of SnO from self-reduction in CO electroreduction reaction (CO RR), stabilizing SnO species in the composite catalyst even after long-term usage. Calculations based on density functional theory signified that the presence of Bi O favored the adsorption of HCOO* intermediate, improved the CO conversion into HCOOH on SnO /Bi O interface. As a result, the SnO /Bi O catalyst attained high performance on CO RR (the highest FE value of 90 % at -1.0 V vs. RHE), suppressing H evolution reaction (HER) at high potentials. In particular, the selectivity of HCOOH remained above 76 % in a wide potential window (from -1.0 to -1.4 V vs. RHE) and a long duration (12 h).
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http://dx.doi.org/10.1002/cssc.202100543DOI Listing
May 2021

[Clinical features of neurogenic bladder with vesicoureteral reflux in children].

Zhongguo Dang Dai Er Ke Za Zhi 2021 Mar;23(3):279-282

Department of Nephrology, Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, China.

Objective: To study the clinical features of vesicoureteral reflux (VUR) in children with neurogenic bladder (NB), and to provide a reference for its early diagnosis and treatment.

Methods: Clinical data were collected from 26 children with NB and urinary tract infection who were admitted to the Department of Pediatric Nephrology from January 2014 to December 2019. According to the presence or absence of VUR, the children were divided into a VUR group with 11 children and a non-VUR group with 15 children. Clinical features were compared between the two groups.

Results: Compared with the non-VUR group, the VUR group had a significantly higher proportion of children with non- urinary tract infection, hydronephrosis (the severity of hydronephrosis increased with the grade of VUR), abnormal Tc-DMSA renal scanning findings, elevated ratios of urinary albumin, urinary IgG and urinary transferrin to creatinine, increased residual urine volume, and increased detrusor leak point pressure ( < 0.05).

Conclusions: When NB children have the clinical manifestations of non- urinary tract infection, hydronephrosis, abnormal Tc-DMSA renal scanning findings, glomerular proteinuria, increased bladder residual urine volume, and high detrusor leak point pressure, such children may already have VUR, and so diagnosis and intervention should be performed as early as possible.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7969185PMC
March 2021

Tumor-associated macrophages promote the metastasis and growth of non-small-cell lung cancer cells through NF-κB/PP2Ac-positive feedback loop.

Cancer Sci 2021 Jun 1;112(6):2140-2157. Epub 2021 May 1.

Department of Radiation Oncology, The First Affiliated Hospital of Soochow University, Suzhou, China.

Non-small-cell lung cancer (NSCLC), with its aggressive biological behavior, is one of the most diagnosed cancers. Tumor-associated inflammatory cells play important roles in the interaction between chronic inflammation and lung cancer, however the mechanisms involved are far from defined. In the present study, by developing an orthotopic NSCLC mouse model based on chronic inflammation, we proved that an inflammatory microenvironment accelerated the growth of orthotopic xenografts in vivo. Tumor-associated macrophages, the most abundant population of inflammatory cells, were identified. Treatment with macrophage-conditioned medium (MCM) promoted the growth and migration of NSCLC cells. Using bioinformatics analysis, we identified downregulated PP2Ac expression in NSCLC cells upon treatment with MCM. We further confirmed that this downregulation was executed in an NF-κB pathway-dependent manner. As IκB kinase (IKK) has been proved to be a substrate of PP2Ac, inhibition on PP2Ac could result in amplification of NF-κB pathway signaling. Overexpression of PP2Ac, or the dominant-negative forms of IKK or IκB, attenuated the acceleration of growth and metastasis by MCM. Using bioinformatics analysis, we further identified that CXCL1 and COL6A1 could be downstream of NF-κB/PP2Ac pathway. Luciferase assay and ChIP assay further confirmed the location of response elements on the promoter regions of CXCL1 and COL6A1. Elevated CXCL1 facilitated angiogenesis, whereas upregulated COL6A1 promoted proliferation and migration.
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http://dx.doi.org/10.1111/cas.14863DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8177805PMC
June 2021

FOLFIRINOX regulated tumor immune microenvironment to extend the survival of patients with resectable pancreatic ductal adenocarcinoma.

Gland Surg 2020 Dec;9(6):2125-2135

Department of Oncology, First Affiliated Hospital of Soochow University, Suzhou, China.

Background: Pancreatic ductal adenocarcinoma (PDAC) is one of the most deadly malignant tumors worldwide due to its ineffective diagnosis and poor prognosis. The longest median overall survival (OS) to PDAC patients has been provided by FOLFIRINOX. It is essential to identify the mechanisms of FOLFIRINOX to gain new insights for the treatment of PDAC.

Methods: We compared gene expression levels of PDAC patients who received neoadjuvant FOLFIRINOX prior to surgery with those of patients who received no neoadjuvant chemotherapy. Bioinformatics analysis was applied to screen differentially expressed genes (DEGs). Three microarray data sets were downloaded to analyze gene expression data between PDAC and adjacent non-tumor tissues. Overlapping DEGs were subjected to Kaplan-Meier survival analysis. The genes relating to poor outcomes and would be decreased after FOLFIRINOX were input into the Oncomine, University of Alabama Cancer (UALCAN), and LinkedOmics databases to analyze the gene expression and regulation networks.

Results: A total of 83 differentially expressed genes (DEGs) were screened and subjected to bioinformatics analysis, which indicated FOLFIRINOX influenced the immune microenvironment of PDAC. Seventy-three genes significantly associated with the OS of PDAC patients. A Venn diagram revealed CXCL5 and PLAU were related to poor outcomes and would decrease after FOLFIRINOX chemotherapy of PDAC patients. It turned out that CXCL5 participated in the immune response-regulating signaling pathway in PDAC patients.

Conclusions: FOLFIRINOX regulated tumor immunity by reducing expression of the immunosuppressive gene CXCL5, laying a foundation for further study of combination therapy of FOLFIRINOX and immunotherapy.
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http://dx.doi.org/10.21037/gs-20-828DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7804558PMC
December 2020

Blocking MAPK/ERK pathway sensitizes hepatocellular carcinoma cells to temozolomide via downregulating MGMT expression.

Ann Transl Med 2020 Oct;8(20):1305

Department of Oncology, the First Affiliated Hospital of Soochow University, Suzhou, China.

Background: Hepatocellular carcinoma (HCC) is the fourth most common malignant tumor in China. Temozolomide (TMZ) is a common chemotherapy drug which can effectively kill HCC cells in vitro. However, it is possible that HCC cells possess intrinsic resistance to TMZ. A key mechanism of TMZ resistance is the overexpression of O6-methylguanine-DNA methyltransferase (MGMT). Studies have shown that MAPK may be related to MGMT expression, U0126 is a highly selective inhibitor of MEK1 and MEK2, which were crucial molecule in cascade of mitogen-activated protein kinase/extracellular signal regulated kinase (MAPK/ERK) pathway. Sorafenib was another widely applicated target drug in HCC which could inhibit multiple kinases including MAPK/ERK. This research was aimed to investigate the efficacy of MAPK/ERK inhibitor U0126 and sorafenib combine with TMZ in the treatment of HCC.

Methods: In HCC cells, MAPK/ERK signaling pathway was blocked by U0126 and sorafenib. The effect of blocking MAPK/ERK signaling pathway on TMZ-induced cytotoxicity was evaluated by MTT assay, flow cytometry and TUNEL assay. DNA damage protein and the expression of MGMT were detected by Western-blot. After the downregulation of MAPK/ERK signaling pathway, MGMT mRNA expression and the protein expression of MGMT were quantified by quantitative real-time polymerase chain reaction (RT-qPCR) and immunofluorescence assay, respectively. HepG2 cells were transfected with an MGMT over expression plasmid. After transfection, the effect of U0126 on TMZ-induced cytotoxicity was evaluated by MTT and Western-Blot in MGMT OE cells. The influence of Sorafenib on TMZ-induced cytotoxicity to HCC cells was also detected by MTT assay.

Results: U0126 can enhance the chemosensitivity of HCC cells to TMZ. At the same time, we also found that U0126 increases the damage to DNA caused by TMZ in HepG2 cells. Moreover, the results from RT-qPCR and Western blot showed that U0126 downregulated MGMT mRNA and MGMT protein expression via blocking MAPK/ERK pathway. Furthermore, after transfection with an MGMT expression plasmid, overexpression of MGMT restored U0126-induced chemosensitivity to TMZ in HCC cells. Sorafenib can also increase the chemosensitivity of HCC cells to TMZ.

Conclusions: Our studies suggest great clinical potential for the utilization of combined U0126 and TMZ in patients with advanced HCC.
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http://dx.doi.org/10.21037/atm-20-5478DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7661899PMC
October 2020

Identification of immune-related genes as prognostic factors in bladder cancer.

Sci Rep 2020 11 12;10(1):19695. Epub 2020 Nov 12.

Department of General Practice, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou, People's Republic of China.

Bladder cancer is one of the most common cancers worldwide. The immune response and immune cell infiltration play crucial roles in tumour progression. Immunotherapy has delivered breakthrough achievements in the past decade in bladder cancer. Differentially expressed genes and immune-related genes (DEIRGs) were identified by using the edgeR package. Gene ontology annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed for functional enrichment analysis of DEIRGs. Survival-associated IRGs were identified by univariate Cox regression analysis. A prognostic model was established by univariate COX regression analysis, and verified by a validation prognostic model based on the GEO database. Patients were divided into high-risk and low-risk groups based on the median risk score value for immune cell infiltration and clinicopathological analyses. A regulatory network of survival-associated IRGs and potential transcription factors was constructed to investigate the potential regulatory mechanisms of survival-associated IRGs. Nomogram and ROC curve to verify the accuracy of the model. Quantitative real-time PCR was performed to validate the expression of relevant key genes in the prognostic model. A total of 259 differentially expressed IRGs were identified in the present study. KEGG pathway analysis of IRGs showed that the "cytokine-cytokine receptor interaction" pathway was the most significantly enriched pathway. Thirteen survival-associated IRGs were selected to establish a prognostic index for bladder cancer. In both TCGA prognostic model and GEO validation model, patients with high riskscore had worse prognosis compared to low riskscore group. A high infiltration level of macrophages was observed in high-risk patients. OGN, ELN, ANXA6, ILK and TGFB3 were identified as hub survival-associated IRGs in the network. EBF1, WWTR1, GATA6, MYH11, and MEF2C were involved in the transcriptional regulation of these survival-associated hub IRGs. The present study identified several survival-associated IRGs of clinical significance and established a prognostic index for bladder cancer outcome evaluation for the first time.
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http://dx.doi.org/10.1038/s41598-020-76688-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7661532PMC
November 2020

Probing the effect of P-doping in polymeric carbon nitride on CO photocatalytic reduction.

Dalton Trans 2020 Nov;49(44):15750-15757

State Key Laboratory of High performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai, 200050, P. R. China. and Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing, 100049, P. R. China.

Developing highly efficient photocatalysts is of crucial importance to solve the energy crisis and global warming issues. In this work, a P-doped polymeric carbon nitride (CN) photocatalyst was synthesized by one-step copolymerization of guanidine hydrochloride and phosphonitrilic chloride trimer. The doping of P in CN was found to alter the electronic structure, enhance the charge separation and transfer, and promote the CO2 adsorption and activation, making it an efficient CO2 photoreduction catalyst. At the optimized P dose, the CO evolution amount on P-doped CN reached 0.349 μmol (30 mg, 3 h), which was 3.5 times that of pure CN. The process of CO2 photoreduction on P-doped CN was investigated by in situ FTIR analysis, revealing that P doping could promote the formation of a CO2- intermediate. A possible mechanism has been proposed, which may provide new insights into the effect of non-metal element doping in CN on its CO2 photocatalytic reduction performance.
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http://dx.doi.org/10.1039/d0dt03229hDOI Listing
November 2020

Highly Efficient and Selective CO Electro-Reduction to HCOOH on Sn Particle-Decorated Polymeric Carbon Nitride.

ChemSusChem 2020 Dec 4;13(23):6442-6448. Epub 2020 Nov 4.

State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai, 200050, P. R. China.

Electrochemical conversion of CO into liquid fuels by efficient and earth-abundant catalysts is of broad interest but remains a great challenge in renewable energy production and environmental remediation. Herein, a Sn particle-decorated polymeric carbon nitride (CN) electrocatalyst was successfully developed for efficient, durable, and highly selective CO reduction to formic acid. High-resolution X-ray photoelectron spectroscopy confirmed that the metallic Sn particles and CN matrix are bound by strong chemical interaction, rendering the composite catalyst a stable structure. More notably, the electronic structure of Sn was well tuned to be highly electron-rich due to the electron transfer from N atoms of CN to Sn atoms via metal-support interactions, which favored the adsorption and activation of CO molecules, promoted charge transport, and thus enhanced the electrochemical conversion of CO . The composite electrocatalyst demonstrated an excellent Faradaic efficiency of formic acid (FE ) up to 96±2 % at the potential of -0.9 V vs. reversible hydrogen electrode, which remained at above 92 % during the electrochemical reaction of 10 h, indicating that the present Sn particle-decorated polymeric carbon nitride electrocatalyst is among the best in comparison with reported Sn-based electrocatalysts.
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http://dx.doi.org/10.1002/cssc.202002184DOI Listing
December 2020

Realizing a ferroelectric state and high pyroelectric performance in antiferroelectric-oxide composites.

Dalton Trans 2020 Jul;49(28):9728-9734

National Laboratory of Solid State Microstructures, Department of Materials Science and Engineering, College of Engineering and Applied Science & Jiangsu Key Laboratory of Artificial Functional Materials & Collaborative Innovation Center of Advanced Microstructures, Nanjing University, Nanjing 210093, China.

We report a robust room temperature ferroelectric (FE) state in (1 - x)Pb0.99Nb0.02[(Zr0.57Sn0.43)0.933Ti0.067]0.98O3-xZnO ((1 - x)PNZST-xZnO) composites, where PNZST shows a predominant antiferroelectric (AFE) nature due to ZnO-induced internal strain. Upon heating, a FE-AFE transition occurs and generates high pyroelectric performance. The composite with x = 0.1 shows a peak pyroelectric coefficient of p = 2450.7 × 10-4 C m-2 K-1 and figures of merit of current responsivity Fi = 926.9 × 10-10 m V-1, voltage responsivity Fv = 1334.3 × 10-2 m2 C-1, and detectivity Fd = 1194.8 × 10-5 Pa-1/2, which are about two orders of magnitude higher than those of most perovskite pyroelectric oxides. More interestingly, the FE-AFE transition temperature, i.e., the temperature corresponding to peak pyroelectric performance, is tunable in a wide temperature range from 30 °C to 65 °C. This work not only provides a promising material candidate for high performance pyroelectric devices, but also an alternative idea to develop ferroelectric and pyroelectric properties based on antiferroelectric materials.
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http://dx.doi.org/10.1039/d0dt01739fDOI Listing
July 2020

Mild generation of surface oxygen vacancies on CeO for improved CO photoreduction activity.

Nanoscale 2020 Jun 3;12(23):12374-12382. Epub 2020 Jun 3.

School of Materials Science and Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, P. R. China.

The vacancy defects of semiconductor photocatalysts play key roles in enhancing their photocatalytic CO reduction activity. In this work, CeO was chosen as a model catalyst and oxygen vacancies were introduced on its surface by a facile and mild oxalic acid treatment followed by moderate heating in N. Such a treatment resulted in a much increased ratio of Ce/Ce in CeO, and the oxygen vacancy-enriched CeO showed remarkably enhanced photocatalytic activity in CO reduction, with CO being the dominant reduction product, whose yield was about 8 times that on the pristine CeO. In situ FT-IR spectra showed that the abundant oxygen vacancies substantially improved the CO adsorption/activation on the surface of CeO, which facilitated the subsequent reduction of CO. However, the carbonates strongly adsorbed on the photocatalyst surface might be the main obstacle to maintaining the high CO reduction activity and stability of CeO with O vacancies.
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http://dx.doi.org/10.1039/d0nr00717jDOI Listing
June 2020

Soluble dietary fibers from black soybean hulls: Physical and enzymatic modification, structure, physical properties, and cholesterol binding capacity.

J Food Sci 2020 Jun 26;85(6):1668-1674. Epub 2020 May 26.

College of Food Science, Heilongjiang Bayi Agricultural Univ., Daqing, Heilongjiang, 163319, China.

We used ultrasound-microwave comodification and enzyme modification (cellulase and hemicellulase) methods to extract soluble dietary fibers (SDFs) from black soybean hulls. Moreover, the structure, physical, and chemical properties, as well as the cholesterol-binding capacity of SDFs before and after modification were analyzed. The average molecular weight of SDFs extracted from raw black soybean hulls was 2.815 × 10  Da. By comparison, the average molecular weight of SDFs from ultrasound-microwave comodified hulls and enzyme-modified hulls decreased by 33.21% and 45.29%, respectively. The water-holding capacity (WHC), water-swelling capacity (WSC), and oil-holding capacity (OHC) of the extracted SDFs modified by the ultrasound-microwave method were 3.79 g/g, 1.39 mL/g, and 1.14 g/g, respectively, a 9.54%, 23.01%, and 17.53% increase from the values of raw SDF. The WHC, WSC, and OHC of SDFs modified via the enzyme method were 3.59 g/g, 1.25 mL/g, and 1.03 g/g, respectively, with a 3.76%, 10.62%, and 6.19% increase when compared to raw SDFs. The cholesterol-binding capacity of SDFs modified via the ultrasound-microwave and enzyme methods was 13.82 and 12.34 mg/g, respectively, with an increase of 47.98% and 32.20% when compared to raw SDFs. The changes in structure and physical and chemical properties were shown to be closely related to the significantly improved cholesterol-binding capacity of the SDFs from modified black soybean hulls. This provides a theoretical basis for subsequent research and development of black soybean hulls products. PRACTICAL APPLICATION: At present, the black soybean hull, a byproduct of general grains, is usually abandoned, but black soybean hull is rich in dietary fiber. Enzymatic modification and ultrasound-microwave comodification were used to treat black soybean hull to prepare small molecular weight, highly active soluble dietary fiber. This research is of great significance to the deep processing of black soybean hull and improvement of the economic benefits of black soybean byproducts.
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http://dx.doi.org/10.1111/1750-3841.15133DOI Listing
June 2020

microRNA-15b contributes to depression-like behavior in mice by affecting synaptic protein levels and function in the nucleus accumbens.

J Biol Chem 2020 05 24;295(20):6831-6848. Epub 2020 Mar 24.

Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China

Major depression is a prevalent affective disorder characterized by recurrent low mood. It presumably results from stress-induced deteriorations of molecular networks and synaptic functions in brain reward circuits of genetically-susceptible individuals through epigenetic processes. Epigenetic regulator microRNA-15b inhibits neuronal progenitor proliferation and is up-regulated in the medial prefrontal cortex of mice that demonstrate depression-like behavior, indicating the contribution of microRNA-15 to major depression. Using a mouse model of major depression induced by chronic unpredictable mild stress (CUMS), here we examined the effects of microRNA-15b on synapses and synaptic proteins in the nucleus accumbens of these mice. The application of a microRNA-15b antagomir into the nucleus accumbens significantly reduced the incidence of CUMS-induced depression and reversed the attenuations of excitatory synapse and syntaxin-binding protein 3 (STXBP3A)/vesicle-associated protein 1 (VAMP1) expression. In contrast, the injection of a microRNA-15b analog into the nucleus accumbens induced depression-like behavior as well as attenuated excitatory synapses and STXBP3A/VAMP1 expression similar to the down-regulation of these processes induced by the CUMS. We conclude that microRNA-15b-5p may play a critical role in chronic stress-induced depression by decreasing synaptic proteins, innervations, and activities in the nucleus accumbens. We propose that the treatment of anti-microRNA-15b-5p may convert stress-induced depression into resilience.
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http://dx.doi.org/10.1074/jbc.RA119.012047DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7242712PMC
May 2020

RNA 6-methyladenosine: a promising molecular target in metabolic diseases.

Cell Biosci 2020 21;10:19. Epub 2020 Feb 21.

4Hangzhou Xixi Hospital Affiliated to Zhejiang Chinese Medical University, Hangzhou, 310023 Zhejiang China.

6-methyladenosine is a prevalent and abundant transcriptome modification, and its methylation regulates the various aspects of RNAs, including transcription, translation, processing and metabolism. The methylation of 6-methyladenosine is highly associated with numerous cellular processes, which plays important roles in the development of physiological process and diseases. The high prevalence of metabolic diseases poses a serious threat to human health, but its pathological mechanisms remain poorly understood. Recent studies have reported that the progression of metabolic diseases is closely related to the expression of RNA 6-methyladenosine modification. In this review, we aim to summarize the biological and clinical significance of RNA 6-methyladenosine modification in metabolic diseases, including obesity, type 2 diabetes, non-alcoholic fatty liver disease, hypertension, cardiovascular diseases, osteoporosis and immune-related metabolic diseases.
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http://dx.doi.org/10.1186/s13578-020-00385-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7035649PMC
February 2020

Electron Configuration Modulation of Nickel Single Atoms for Elevated Photocatalytic Hydrogen Evolution.

Angew Chem Int Ed Engl 2020 Apr 9;59(17):6827-6831. Epub 2020 Mar 9.

State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai, 200050, P. R. China.

The emerging metal single-atom catalyst has aroused extensive attention in multiple fields, such as clean energy, environmental protection, and biomedicine. Unfortunately, though it has been shown to be highly active, the origins of the activity of the single-atom sites remain unrevealed to date owing to the lack of deep insight on electronic level. Now, partially oxidized Ni single-atom sites were constructed in polymeric carbon nitride (CN), which elevates the photocatalytic performance by over 30-fold. The 3d orbital of the partially oxidized Ni single-atom sites is filled with unpaired d-electrons, which are ready to be excited under irradiation. Such an electron configuration results in elevated light response, conductivity, charge separation, and mobility of the photocatalyst concurrently, thus largely augmenting the photocatalytic performance.
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http://dx.doi.org/10.1002/anie.201914565DOI Listing
April 2020

A randomized controlled trial on ambulatory blood pressure lowering effect of CPAP in patients with obstructive sleep apnea and nocturnal hypertension.

Blood Press 2020 02 7;29(1):21-30. Epub 2019 Nov 7.

Centre for Epidemiological Studies and Clinical Trials, Shanghai Key Laboratory of Hypertension, the Shanghai Institute of Hypertension, Department of Hypertension, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.

In a randomised controlled trial, we investigated the blood pressure (BP) lowering effect of continuous positive airway pressure (CPAP) in patients with moderate-severe obstructive sleep apnoea syndrome (OSAS, an apnoea-hypopnoea index, AHI of 15 or higher) and nocturnal hypertension (night-time systolic/diastolic BP ≥120/70 mmHg). Sixty patients were randomly assigned to CPAP or sham CPAP, while maintaining their antihypertensive treatment. Ambulatory BP monitoring was performed at baseline (first run-in visit) and the end of follow-up. Clinic and home BP were measured at baseline and each of the monthly follow-up visits. Of the 60 patients, 47 completed the 3-month study. CPAP ( = 26), compared with sham CPAP ( = 21), slightly and non-significantly reduced 24-h systolic/diastolic BP by -2.8/-2.5 mmHg ( ≥ 0.27), with a slightly greater between-group difference in the daytime (-4.0/-2.8 mmHg,  ≥ 0.29) than night-time (-0.2/-1.5 mmHg,  ≥ 0.50). The CPAP treatment did not significantly influence clinic or home BP during follow-up ( ≥ 0.27). Nonetheless, simple and partial correlation analyses showed that the ambulatory BP lowering effect was dependent on the daytime pulse rate at baseline ( ≥ 0.47,  ≤ 0.01). In patients with a daytime pulse rate greater than 85 beats/min, the mean changes in daytime systolic BP were significantly greater in the CPAP ( = 10) than sham CPAP group ( = 11), with a between-group mean difference of -10.1 mmHg ( = 0.048). The CPAP treatment did not show significant ambulatory BP lowering effect in patients with moderate-severe OSAS and nocturnal hypertension. However, it may be effective in lowering daytime BP in patients with a faster pulse rate.
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http://dx.doi.org/10.1080/08037051.2019.1686343DOI Listing
February 2020

Evaluation of the antioxidant effects of acid hydrolysates from Auricularia auricular polysaccharides using a Caenorhabditis elegans model.

Food Funct 2019 Sep 16;10(9):5531-5543. Epub 2019 Aug 16.

College of Life Sciences, China Jiliang University, Hangzhou, Zhejiang 310018, China.

Caenorhabditis elegans is an important model organism for studying stress response mechanisms and identifying genetic pathways that influence longevity. The present study was designed to explore the in vivo-antioxidant potential and the probable mechanism of acid hydrolysates prepared from A. auricula polysaccharides (AAPHs-F) with the optimal acid hydrolysis conditions using Box-Behnken design, and C. elegans was used as a model organism. The effects of AAPHs-F on the locomotory behavior, lifespan, activities of antioxidant-related enzymes and levels of antioxidants in C. elegans were studied. In addition, the potential of AAPHs-F in up-regulating the expression of antioxidant-related genes in C. elegans, such as daf-16, skn-1, sod-1, sod-2 and sir-2.1, and the inhibition of cell apoptosis of C. elegans were also discussed. The results indicated that AAPHs-F could significantly increase the U-Turn frequency of nematodes, extend their lifespan, enhance antioxidant systems including superoxide dismutase (SOD) by 70.60%, catalase (CAT) by 73.45% and glutathione reductase (GR) by 258.68% (p < 0.01), increase the level of glutathione (GSH) by 110.22% (p < 0.01), and decrease the level of reactive oxygen species (ROS) and malondialdehyde (MDA) by 31.86% and 46.16% (p < 0.01), respectively. Quantitative real-time polymerase chain reaction (qRT-PCR) results showed that AAPHs-F could up-regulate mRNA expression levels of daf-16, skn-1, sir, sod-1 and sod-2 in wild-type C. elegans (>1.3 fold) when treated at a concentration of 0.1 mg mL (p < 0.05 or p < 0.01). AAPHs-F was concluded to be heteropolysaccharides composed of mannose, glucose and galactose with a molar ratio of 12.7 : 3.25 : 1. The molecular weight of AAPHs-F was determined to be 885.37 Da. Furthermore, AAPHs-F is mainly formed of (1 → 3)-linked-α-d-glucopyranose, and carboxyl or acetamide is present in the molecule. In summary, our studies provide evidence that AAPHs-F helps improve the antioxidant defense system, and up-regulation of stress and longevity related genes suggests the possible involvement of these genes in the prevention of stress damage in C. elegans.
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http://dx.doi.org/10.1039/c8fo02589dDOI Listing
September 2019

Inflammation Promotes Progression of Pancreatic Cancer Through WNT/β-Catenin Pathway-Dependent Manner.

Pancreas 2019 09;48(8):1003-1014

From the Department of Oncology, the First Affiliated Hospital of Soochow University.

Objective: Identify the molecular mechanism of inflammatory stimuli induced pancreatic cancer progression.

Methods: RNA-seq, microarray assay and bioinformatics analyses were used to identify differentially expressed genes. Immunohistochemical staining was performed to evaluate CD68, CD163, β-catenin, CD103, CCL3 markers. Quantitative real-time polymerase chain reaction (qRT-PCR), luciferase reporter assay, apoptosis assay, wound healing assay and immunofluorescence were performed to study the relationship of inflammatory stimuli and WNT/β-catenin pathway.

Results: Differentially expressed genes of macrophage-conditioned medium-treated pancreatic cancer cells were related with WNT/β-catenin pathway. Inflammatory stimuli could activate WNT/β-catenin signaling pathway. In 106 pancreatic cancer patients, nuclear β-catenin expression of CD68-high group was much higher than CD68-low group (P < 0.05), as same as CD163 (P < 0.05). Inflammatory stimuli downregulated the expression of CCL3 via WNT/β-catenin pathway and inhibited the chemotaxis of CD103 dendritic cells. Six pancreatic cancer prognosis associating genes were upregulated by inflammatory stimuli via WNT/β-catenin pathway. Transforming growth factor-β promoted malignant biological behavior of pancreatic cancer cells through WNT/β-catenin pathway-dependent mechanism.

Conclusions: Our present study provided a novel mechanism involved in the inflammation-driven cancer progression through tumor immune escape and downstream gene regulation of WNT/β-catenin pathway-dependent manner.
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http://dx.doi.org/10.1097/MPA.0000000000001386DOI Listing
September 2019

Traditional Uses, Chemical Constituents and Biological Activities of Plants from the Genus Thymus.

Chem Biodivers 2019 Sep 6;16(9):e1900254. Epub 2019 Sep 6.

School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing, 100102, P. R. China.

The genus Thymus (Lamiaceae) comprises about 214 species throughout the world, mainly found in North Africa, Europe, and temperate Asia zone. They are traditionally used as food additives and folk medicines. This review comprehensively summarizes information about traditional uses, chemical constituents, and biological activities of this genus and provides recommendations for future investigations. All information was gathered from scientific databases including Google Scholar, Sci-Finder, Web of Science, ScienceDirect, and CNKI. Volatile oils are the most concerned constituents of this genus. Flavonoids, phenylpropanoids, tannins, organic acids, terpenoids, and phytosterols were also summarized. This genus plants possessed a variety of activities including antimicrobial, antioxidant, anti-inflammatory, cytotoxic, analgesic, and antidiabetic. In brief, this review will be helpful to provide valuable data for explorations and create more interests towards Thymus genus in the future.
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http://dx.doi.org/10.1002/cbdv.201900254DOI Listing
September 2019

Chemotherapy-Induced Extracellular Vesicle miRNAs Promote Breast Cancer Stemness by Targeting .

Cancer Res 2019 07 22;79(14):3608-3621. Epub 2019 May 22.

Department of Pathology, University of California, San Diego; La Jolla, California.

Cancer-secreted, extracellular vesicle (EV)-encapsulated miRNAs enable cancer cells to communicate with each other and with noncancerous cells in tumor pathogenesis and response to therapies. Here, we show that treatment with a sublethal dose of chemotherapeutic agents induces breast cancer cells to secrete EV with the capacity to stimulate a cancer stem-like cell (CSC) phenotype, rendering cancer cells resistance to therapy. Chemotherapy induced breast cancer cells to secrete multiple EV miRNAs, including miR-9-5p, miR-195-5p, and miR-203a-3p, which simultaneously targeted the transcription factor One Cut Homeobox 2 (ONECUT2), leading to induction of CSC traits and expression of stemness-associated genes, including , and . Inhibition of these miRNAs or restoration of ONECUT2 expression abolished the CSC-stimulating effect of EV from chemotherapy-treated cancer cells. In mice bearing xenograft mammary tumors, docetaxel treatment caused elevations of miR-9-5p, miR-195-5p, and miR-203a-3p in circulating EV and decreased ONECUT2 expression and increased levels of stemness-associated genes. These effects following chemotherapy were diminished in tumors deficient in exosome secretion. In human breast tumors, neoadjuvant chemotherapy decreased ONECUT2 expression in tumor cells. Our results indicate a mechanism by which cancer cells communicate with each other and self-adapt to survive in response to cytotoxic treatment. Targeting these adaptation mechanisms along with chemotherapy, such as by blocking the EV miRNA-ONECUT2 axis, represents a potential strategy to maximize the anticancer effect of chemotherapy and to reduce chemoresistance in cancer management. SIGNIFICANCE: These findings reveal a critical mechanism of resistance to chemotherapy by which breast cancer cells secrete miRNA-containing extracellular vesicles to stimulate cancer stem cell-like features.
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http://dx.doi.org/10.1158/0008-5472.CAN-18-4055DOI Listing
July 2019

A randomized cross-over study on the blood pressure lowering effect of the combined passive head-up and -down movement with Device-Guided slow breathing.

Blood Press 2019 10 9;28(5):291-299. Epub 2019 May 9.

Centre for Epidemiological Studies and Clinical Trials, Shanghai Key Laboratory of Hypertension, The Shanghai Institute of Hypertension, Department of Hypertension, Ruijin Hospital, Shanghai Jiaotong University School of Medicine , Shanghai , China.

Baroreflex emerges as a therapeutic target of hypertension. We investigated blood pressure (BP) lowering effect of the combined passive head-up and -down movement with device-guided slow breathing in untreated mild hypertension or high-normal BP. In a randomized, cross-over trial, untreated subjects with an ambulatory systolic/diastolic BP of 125-140/80-90 mmHg and a clinic BP of 130-150/80-90 mmHg were randomized to intervention treatment with head movement and slow breathing or sham control, and then crossed over. Both treatments consisted of 1-week preparation, 2-week treatment, and 1-week recovery. During the 2-week treatment, subjects were treated for a session of 20 min/day. BP, pulse rate and respiration were measured before and after each treatment session. Ambulatory BP monitoring was performed at baseline and the end of the 2-week treatments' period, and home BP monitoring in the morning and evening for the whole 8-week follow-up period. 14 subjects completed the study. The intervention treatment, compared to control, reduced respiration rate by -2.1 breaths/min (95% CI -2.9 to -1.2,  = .0001), but not clinic BP and pulse rate ( ≥ .67). The intervention treatment, compared to control, significantly reduced nighttime systolic/diastolic blood pressure by -5.63/-3.82 mm Hg ( ≤ .01) but not 24-h or daytime ambulatory blood pressure ( ≥ .69). Home BP decreased with the intervention treatment, but the between-treatment difference was not statistically significant ( ≥ .27). The combined head movement with slow breathing did not influence 24-h BP, but reduced nighttime BP in untreated mild hypertension or high-normal BP.
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http://dx.doi.org/10.1080/08037051.2019.1613884DOI Listing
October 2019

New spectral reduction algorithm for echelle spectrometer in laser-induced breakdown spectroscopy.

Opt Express 2018 Dec;26(26):34131-34141

In this work, a new spectral reduction algorithm for the echelle spectrometer was proposed. Unlike conventional approaches, the key concept in this algorithm is to model the spectrogram rather the spectrometer, which makes the algorithm more adaptive to different designs. This algorithm also introduces a dynamic adjusting procedure for generating optimized spectra from laser-induced plasmas. This additional step improved the spectrum stability and absolute line intensity of the spectrum and yielded better quantification performance. Experimental results demonstrated that the quantification results of analyzing aluminum alloy samples were improved using this new algorithm.
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http://dx.doi.org/10.1364/OE.26.034131DOI Listing
December 2018

Hypoglycemic Effect of the Degraded Polysaccharides from the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Agaricomycetes).

Int J Med Mushrooms 2019 ;21(10):1033-1042

College of Life Sciences, China Jiliang University, Hangzhou, Zhejiang, 310018, China.

Auricularia auricula-judae is an important culinary-medicinal mushroom. The A. auricula-judae polysaccharides (AAPs) were prepared from A. auricula-judae in the early stage through alkali extraction and deproteination with the Sevag method, and optimal acid hydrolysis conditions were established by Box-Behnken to prepare the degraded polysaccharides (AAPs-F) from AAPs. In this study, a nonenzymatic glycosylation reaction system was used for the evaluation of the inhibitory effects on the formation of advanced glycation end products (AGEs). In addition, high glucose resistance was assessed by glucose consumption of HepG2 cells and the lifespan of Caenorhabditis elegans under high sugar stress. It was found that both 0.5 mg·mL-1 AAPs and 0.2 mg·mL-1 AAPs-F could significantly inhibit AGE formation in short- and long-term glycosylation (P < .05) in a dose-dependent manner, determined by ultraviolet and fluorospectrophotometry. It indicated activity against AGE formation for different concentrations of AAPs and AAPs-F. AAPs-F at 0.5 mg·mL-1 significantly enhanced the glucose absorption of HepG2 cells by 24.4% (P < .05) in a dose-dependent manner at 24 h, and markedly extended the lifespan of C. elegans by 32.9% (P < 0.05) under high sugar stress conditions. This study demonstrated that the derived hydrolysates produced by the hydrolysis of acid had a prominent effect on the inhibition of AGE formation and relieved the stress state caused by high sugar levels.
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http://dx.doi.org/10.1615/IntJMedMushrooms.2019032353DOI Listing
June 2020

Antioxidant and anti-diabetic effects of Auricularia auricular polysaccharides and their degradation by artificial gastrointestinal digestion - Bioactivity of Auricularia auricular polysaccharides and their hydrolysates.

Acta Sci Pol Technol Aliment 2018 Jul-Sep;17(3):277-288

College of Life Sciences, China Jiliang University, Xueyuan Street, Xiasha, Hangzhou, Zhejiang, China.

Background: Auricularia auricular polysaccharides (AAPs) derived from the dried fruit body of A. auricular are valuable compounds with many bioactivities. This research aimed to investigate the antioxidant and anti- diabetic activities of these polysaccharides and their artificial gastrointestinal fluid hydrolysates (AAPHs).

Methods: Artificially simulated gastrointestinal fluid was used to obtain polysaccharide-de- rived fragments, and a rat model of type 2 diabetes mellitus (T2DM) using a high-fat diet and low-dose streptozotocin (STZ) was established to assess their antioxidant and anti-diabetic effects.

Results: It was found that AAPs and AAPHs were both heteropolysaccharides and were comprised of arab- inose, xylose, mannose, 2-deoxy-glucose, glucose and glucosamine, but at different mole ratios. AAPHs was purified by Sephadex G-100 chromatography to produce three fractions, namely, AAPHs1, AAPHs2, and AAPHs3. The molecular weights of these three fractions were 320, 169, and 62 kDa respectively. Both AAPs and AAPHs exhibited the evident ability to enhance the activities of antioxidant enzymes and the level of GSH, while increasing the content of liver glycogen and plasma C-peptide compared with the diabetic model group (p < 0.05). Furthermore, AAPHs could cause a marked improvement in glucose-stimulated GLP-1 secretion from 0 min to 30 min (p < 0.05).

Conclusions: The possible mechanism was that AAPHs could partly restore the STZ-induced impairment of GLP-1 secretion, and inhibit the oxidative stress pathway, and thereby alleviate the progression of diabetes. This data demonstrated that the molecular mole ratio and molecular weight had a definite effect on antioxi- dant and anti-diabetic activities.
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http://dx.doi.org/10.17306/J.AFS.0557DOI Listing
January 2019