Publications by authors named "Meng Li"

2,983 Publications

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A predictive nomogram: a cross-sectional study on a simple-to-use model for screening 12-year-old children for severe caries in middle schools.

BMC Oral Health 2021 Sep 20;21(1):457. Epub 2021 Sep 20.

Department of Preventive Dentistry, State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan, China.

Background: A nomogram is a tool that transforms complex regression equations into simple and visual graphs and enables clinicians and patients to conveniently compute output probabilities without needing medical knowledge and complex formulas. The aim of this study was to develop and validate a predictive nomogram to screen for severe caries among 12-year-old children based on risk factors in Sichuan Province, China.

Methods: A cross-sectional study of 4573 12-year-olds was conducted up to May 2016 in middle schools from three districts and three counties in Sichuan Province, China. All the children underwent oral examinations and completed questionnaires to assess general information, oral impacts on daily performance, dietary habits, subjective health conditions, history of dental trauma, frequency of toothache, dental visits, and knowledge, attitudes, and behaviours toward oral hygiene. Univariate analysis and multivariate logistic regression analysis were used to determine which variables were significantly associated with severe caries (operationalized as DMFT ≥ 3). A nomogram was developed and validated by using the 'rms' package and two cross-validation methods.

Results: Severe caries was found in 537 of the 4573 children (11.74%). Multivariate logistic regression analysis revealed that the following variables predicted a higher risk of severe caries: 'female' [odds ratio (OR) = 1.985, 95% confidence interval (95% CI): 1.63-2.411], 'urban' (OR = 2.389, 95% CI: 1.96-2.91), 'non-only child' (OR = 1.317, 95% CI: 1.07-1.625), 'very poor self-assessment of oral health status' (OR = 2.157, 95% CI: 1.34-3.467) and 'visited a dentist less than 6 months' (OR = 1.861, 95% CI: 1.38-2.505). Multivariate logistic regression analysis also indicated that the following variables predicted a lower risk of severe caries: 'middle level of urbanization' (OR = 0.395, 95% CI: 0.32-0.495) and 'high level of urbanization' (OR = 0.466, 95% CI: 0.37-0.596). Both the fivefold and leave-one-out cross-validation methods indicated that the nomogram model built by these 6 variables displayed good disease recognition ability.

Conclusions: The nomogram was a simple-to-use model to screen children for severe caries. This model was found to facilitate non-dental professionals in assessing risk values without oral examinations and making referrals to dental professionals.
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http://dx.doi.org/10.1186/s12903-021-01819-2DOI Listing
September 2021

Predictive Value of Peripheral Blood Neutrophil-To-Lymphocyte Ratio and Platelet-To-Lymphocyte Ratio on Patient Survival with Peritoneal Dialysis.

Clin Lab 2021 Sep;67(9)

Background: This study is to explore the predictive value of peripheral blood neutrophil-to-lymphocyte ratio (NLR) and platelet-to-lymphocyte ratio (PLR) on the prognosis of patients with peritoneal dialysis (PD).

Methods: A total of 378 patients who underwent PD from July 2004 to November 2019 were selected as the research subjects. According to whether death occurred during the follow-up period, they were divided into death group (86 cases) and survival group (292 cases). The differences in clinical indicators between the two groups were compared, and the multivariate Cox regression model and receiver operating characteristic curve (ROC) were used to analyze and summarize the factors affecting the prognosis of PD patients.

Results: Compared with the survival group, there were significant differences in age, lymphocytes, NLR, PLR, and combined cerebrovascular disease between the death group and the survival group (p < 0.05). Multivariate Cox regression analysis showed that advanced age (HR = 1.055, 95% CI: 1.038 - 1.072), increased NLR (HR = 1.136, 95% CI: 1.067 - 1.210), and increased PLR (HR = 1.184, 95% CI: 1.018 - 3.026) were risk factors for all-cause death in PD patients. The results showed that the area under the ROC curve (AUC) of NLR and PLR for predicting all-cause death of PD patients were 0.698 and 0.659, respectively, the sensitivity was 69.77%, and the specificity was 66.78% and 58.56%, respectively. The optimal critical values were NLR ≥ 3.71 and PLR ≥ 149.28. Taking the best cutoff value of the ROC curve as the threshold, it showed that the cumulative survival rate of patients with NLR ≥ 3.71 was significantly lower than that of patients with NLR < 3.71 (Log rank 2 = 37.551, p = 0.000). It also showed that the cumulative survival rate of patients with PLR ≥ 149.28 was lower than that of patients with PLR < 149.28 (Log rank 2 =23.686, p = 0.000).

Conclusions: NLR and PLR have a good predictive effect on the prognosis of PD patients.
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http://dx.doi.org/10.7754/Clin.Lab.2021.210124DOI Listing
September 2021

Rational integration of defense and repair synergy on PEEK osteoimplants via biomimetic peptide clicking strategy.

Bioact Mater 2022 Feb 10;8:309-324. Epub 2021 Jul 10.

Department of Orthopaedics, The First Affiliated Hospital of Soochow University, 188 Shizi Street, Suzhou, 215006, Jiangsu, China.

Polyetheretherketone (PEEK) has been widely used as orthopedic and dental materials due to excellent mechanical and physicochemical tolerance. However, its biological inertness, poor osteoinduction, and weak antibacterial activity make the clinical applications in a dilemma. Inspired by the mussel adhesion mechanism, here we reported a biomimetic surface strategy for rational integration and optimization of anti-infectivity and osteo-inductivity onto PEEK surfaces using a mussel foot proteins (Mfps)-mimic peptide with clickable azido terminal. The peptide enables mussel-like adhesion on PEEK biomaterial surfaces, leaving azido groups for the further steps of biofunctionalizations. In this study, antimicrobial peptide (AMP) and osteogenic growth peptide (OGP) were bioorthogonally clicked on the azido-modified PEEK biomaterials to obtain a dual-effect of host defense and tissue repair. Since bioorthogonal clicking allows precise collocation between AMP and OGP through changing their feeding molar ratios, an optimal PEEK surface was finally obtained in this research, which could long-term inhibit bacterial growth, stabilize bone homeostasis and facilitate interfacial bone regeneration. In a word, this upgraded mussel surface strategy proposed in this study is promising for the surface bioengineering of inert medical implants, in particular, achieving rational integration of multiple biofunctions to match clinical requirements.
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http://dx.doi.org/10.1016/j.bioactmat.2021.07.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8427090PMC
February 2022

Air flow rate and pressure control approach for the air supply subsystems in PEMFCs.

ISA Trans 2021 Sep 4. Epub 2021 Sep 4.

Department of Control Science and Engineering, Jilin University, Changchun 130022, China; Department of Control Science and Engineering, Tongji University, Shanghai 200092, China.

Simultaneous control of the air flow rate and the cathode pressure plays an essential role in enhancing the performance and prolonging the service life of a polymer electrolyte membrane fuel cell (PEMFC). However, this is challenging to implement in practice due to the high complexity and coupled dynamics of the air supply subsystem in the PEMFC. This work, based on a simplified control-oriented model, puts forward a disturbance-observer-based multi-output feedback control strategy to address the issue of synergistically controlling the air flow rate and cathode pressure. The disturbances (Including humidity, temperature, water vapor, etc.) are estimated through an extended state observer (ESO). The final control law, which takes the disturbances observation into account, is designed through feedback linearization technique. A complex, high-order, nonlinear model is used as the high-fidelity plant in the validation process, and a series of simulations are implemented under different operational conditions. The outcomes show that the proposed control strategy offers a rapid dynamic response, precise steady-state tracking performance, and strong robustness against disturbances and unmodeled dynamics.
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http://dx.doi.org/10.1016/j.isatra.2021.08.046DOI Listing
September 2021

Amorphous cobalt oxide decorated halloysite nanotubes for efficient sulfamethoxazole degradation activated by peroxymonosulfate.

J Colloid Interface Sci 2021 Sep 9;607(Pt 1):857-868. Epub 2021 Sep 9.

School of Environmental Science and Engineering, Hebei University of Science and Technology, Shijiazhuang 050018, China; Institute for Ecological Research and Pollution Control of Plateau Lakes, Institute of International Rivers and Eco-Security, School of Ecology and Environmental Science, Yunnan University, Kunming 650504, China. Electronic address:

In this study, a new hollow nanotube material, 30% Co-CHNTs was prepared by the impregnation-chemical reduction-calcination method. This material can be used as a peroxymonosulfate (PMS) activator to catalyse the degradation of sulfamethoxazole (SMX). The best reaction conditions that correspond to the degradation rate of SMX, up to 97.5%, are as follows: the concentration of SMX is 10 mg L, the amount of catalyst is 0.20 g L, the dosage is 1.625 mM, and the solution pH is 6.00. X-ray photoelectron spectroscopy (XPS) and inductively coupled plasma optical emission spectrometry (ICP-OES) show that the calcined composites mainly stimulate an increase in the content of bivalent cobalt in PMS and reduce the leaching of cobalt ions after the reaction. Additionally, the 30% Co-CHNTs + PMS reaction system exhibits a reasonable SMX degradation rate in a natural organic matter solution and excellent stability after three repeated experiments. Furthermore, the possible degradation mechanism in the 30% Co-CHNTs + PMS reaction system was analysed through electron paramagnetic resonance (EPR) and free-radical capture experiments, and it was observed that the non-radical degradation of O plays a leading role in SMX degradation. Finally, according to the nine degradation intermediates detected by liquid chromatography-mass spectrometry (LC-MS), four possible SMX degradation routes were proposed. This study proved that a 30% Co-CHNTs heterogeneous catalyst is easily prepared, inexpensive, and environmentally friendly and has potential application in antibiotic wastewater treatment.
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http://dx.doi.org/10.1016/j.jcis.2021.08.168DOI Listing
September 2021

Challenges in tin perovskite solar cells.

Phys Chem Chem Phys 2021 Sep 17. Epub 2021 Sep 17.

Department of Novel Materials and Interfaces for Photovoltaic Solar Cells, Helmholtz-Zentrum Berlin für Materialien und Energie GmbH, Hahn-Meitner-Platz 1, 14109 Berlin, Germany.

Perovskite solar cells are the rising star of third-generation photovoltaic technology. With a power conversion efficiency of 25.5%, the record efficiency is close to the theoretical maximum efficiency of a single-junction solar cell. However, lead toxicity threatens commercialization efforts and market accessibility. In this context, Sn-based perovskites are a safe alternative. Nevertheless, the efficiency of Sn-based devices falls far behind the efficiency of Pb-based counterparts. This concise review sheds light on the challenges that the field faces toward making Sn-based perovskites the perovskite photovoltaic benchmark. We identified four key challenges: materials and solvents, film formation, Sn(II) oxidation, and energy band alignment. We illustrate every single challenge and highlight the most successful attempts to overcome them. Finally, we provide our opinion on the most promising trends of this field in the future.
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http://dx.doi.org/10.1039/d1cp02596aDOI Listing
September 2021

Enhancing Antigen Presentation and Inducing Antigen-Specific Immune Tolerance with Amphiphilic Peptides.

J Immunol 2021 Sep 15. Epub 2021 Sep 15.

Department of Chemical Engineering and Materials Science, Wayne State University, Detroit, MI;

Ag-specific immunotherapy to restore immune tolerance to self-antigens, without global immune suppression, is a long-standing goal in the treatment of autoimmune disorders such as type 1 diabetes (T1D). However, vaccination with autoantigens such as insulin or glutamic acid decarboxylase have largely failed in human T1D trials. Induction and maintenance of peripheral tolerance by vaccination requires efficient autoantigen presentation by APCs. In this study, we show that a lipophilic modification at the N-terminal end of CD4 epitopes (lipo-peptides) dramatically improves peptide Ag presentation. We designed amphiphilic lipo-peptides to efficiently target APCs in the lymph nodes by binding and trafficking with endogenous albumin. Additionally, we show that lipophilic modification anchors the peptide into the membranes of APCs, enabling a bivalent cell-surface Ag presentation. The s.c. injected lipo-peptide accumulates in the APCs in the lymph node, enhances the potency and duration of peptide Ag presentation by APCs, and induces Ag-specific immune tolerance that controls both T cell- and B cell-mediated immunity. Immunization with an amphiphilic insulin B chain 9-23 peptide, an immunodominant CD4 T cell epitope in NOD mice, significantly suppresses the activation of T cells, increases inhibitory cytokine production, induces regulatory T cells, and delays the onset and lowers the incidence of T1D. Importantly, treatment with a lipophilic β-cell peptide mixture delays progression to end-stage diabetes in acutely diabetic NOD mice, whereas the same doses of standard soluble peptides were not effective. Amphiphilic modification effectively enhances Ag presentation for peptide-based immune regulation of autoimmune diseases.
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http://dx.doi.org/10.4049/jimmunol.1901301DOI Listing
September 2021

Novel Pyrazolo[3,4-]pyrimidin-4-one Derivatives as Potential Antifungal Agents: Design, Synthesis, and Biological Evaluation.

J Agric Food Chem 2021 Sep 15. Epub 2021 Sep 15.

School of Science, Anhui Agricultural University, Hefei 230036, China.

Plant pathogenic fungi seriously threaten agricultural production. There is an urgent need to develop novel fungicides with low toxicity and high efficiency. In this study, we designed and synthesized 44 pyrazolo[3,4-]pyrimidin-4-one derivatives and evaluated them for their fungicidal activities. The bioassay data revealed that most of the target compounds possessed moderate to high in vitro antifungal activities. Especially compound exhibited remarkable antifungal activity against with an EC value of 1.25 mg/L, close to that of commercial fungicide (EC = 0.96 mg/L) and (EC = 1.91 mg/L). Moreover, compound possessed prominent protective activity against in vivo for 24 h (95.23%) and 48 h (93.78%), comparable to positive control (24 h (96.63%); 48 h (93.23%)). Subsequent studies indicated that compound may impede the growth and reproduction of by affecting the morphology of mycelium, destroying cell membrane integrity, and increasing cell membrane permeability. In addition, the application of compound did not injure the growth or reproduction of Italian bees. This study revealed that compound is expected to be developed for efficient and safe agricultural fungicides.
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http://dx.doi.org/10.1021/acs.jafc.1c02454DOI Listing
September 2021

Time-resolved analysis of photoluminescence at a single wavelength for ratiometric and multiplex biosensing and bioimaging.

Chem Sci 2021 Aug 26;12(33):11020-11027. Epub 2021 Jul 26.

State Key Laboratory of Organic Electronics and Information Displays, Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials (IAM), Institute of Flexible Electronics (Future Technology), Nanjing University of Posts & Telecommunications Nanjing 210023 P. R. China

Simultaneous analysis of luminescence signals of multiple probes can improve the accuracy and efficiency of biosensing and bioimaging. Analysis of multiple signals at different wavelengths usually suffers from spectral overlap, possible energy transfer, and difference in detection efficiency. Herein, we reported a polymeric luminescent probe, which was composed of a phenothiazine-based fluorescent compound and a phosphorescent iridium(iii) complex. Both luminophores emitted at around 600 nm but their luminescence lifetimes are 160 times different, allowing time-resolved independent analysis. As the fluorescence was enhanced in response to oxidation by hypochlorite and the phosphorescence was sensitive toward oxygen quenching, a four-dimensional relationship between luminescence intensity, fluorescence/phosphorescence ratio, hypochlorite concentration, and oxygen content was established. In cellular imaging, time-resolved photoluminescence imaging microscopy clearly showed the independent fluorescence response toward hypochlorite and phosphorescence response toward oxygen in separated time intervals. This work opens up a new idea for the development of multiplex biosensing and bioimaging.
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http://dx.doi.org/10.1039/d1sc02811aDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8386655PMC
August 2021

Disabling de novo DNA methylation in embryonic stem cells allows an illegitimate fate trajectory.

Proc Natl Acad Sci U S A 2021 Sep;118(38)

Wellcome-MRC Cambridge Stem Cell Institute, Jeffrey Cheah Biomedical Centre, University of Cambridge, Cambridge CB2 0AW, United Kingdom;

Genome remethylation is essential for mammalian development but specific reasons are unclear. Here we examined embryonic stem (ES) cell fate in the absence of de novo DNA methyltransferases. We observed that ES cells deficient for both and are rapidly eliminated from chimeras. On further investigation we found that in vivo and in vitro the formative pluripotency transition is derailed toward production of trophoblast. This aberrant trajectory is associated with failure to suppress activation of encodes a bHLH transcription factor expressed in the placenta. Misexpression of in ES cells provokes transdifferentiation to trophoblast-like cells. Conversely, deletion rescues formative transition of mutants and improves contribution to chimeric epiblast. Thus, de novo DNA methylation safeguards against ectopic activation of However, -deficient cells remain defective in ongoing embryogenesis. We surmise that multiple developmental transitions may be secured by DNA methylation silencing potentially disruptive genes.
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http://dx.doi.org/10.1073/pnas.2109475118DOI Listing
September 2021

Interleukin-35 inhibits lipopolysaccharide-induced endothelial cell activation by downregulating inflammation and apoptosis.

Exp Cell Res 2021 Sep 8;407(2):112784. Epub 2021 Sep 8.

Department of Cardiology, The Second Affiliated Hospital of Nanchang University, Nanchang, 330006, China. Electronic address:

Inflammation is an essential factor contributing to sepsis-induced endothelial cell (EC) activation. Interleukin-35 (IL-35) is an anti-inflammatory/immunosuppressive cytokine that exerts protective effects on many inflammatory diseases. In this study, we investigated the effects of IL-35 on lipopolysaccharide (LPS)-induced EC activation and the potential underlying mechanism. Human umbilical vein endothelial cells (HUVECs) were incubated with LPS (1 μg/ml) for 24 h and then cocultured with different concentrations (0, 1, 10, or 100 ng/ml) of recombinant human IL-35 (rhIL-35) for 12 h. Flow cytometry analysis revealed that IL-35 inhibited LPS-induced HUVEC apoptosis in a dose-dependent manner. RT-qPCR and Western blot analyses showed significantly higher mRNA and protein levels of the adhesion molecules intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) and the inflammatory factors IL-6 and IL-8 in the LPS group than in the control group. These changes were alleviated by IL-35 treatment, suggesting that IL-35 protects ECs by downregulating inflammation. Furthermore, IL-35 induced signal transducer and activator of transcription 1 (STAT1) and STAT4 activation and promoted their interaction. Blocking STAT1 or STAT4 expression by fludarabine (STAT1 inhibitor) treatment or siRNA-STAT4-interfering fragment transfection inhibited the protective effect of IL-35 on ECs. Moreover, we observed a similar protective effect of IL-35 treatment on ECs in a mouse sepsis model induced by intraperitoneal LPS injection. This study indicated that IL-35 exerts anti-inflammatory and antiapoptotic effects on LPS-induced EC activation by activating the STAT1 and STAT4 signaling pathways.
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http://dx.doi.org/10.1016/j.yexcr.2021.112784DOI Listing
September 2021

Spatial Threshold Effect of Industrial Land Use Efficiency on Industrial Carbon Emissions: A Case Study in China.

Int J Environ Res Public Health 2021 Sep 5;18(17). Epub 2021 Sep 5.

College of Economics and Management, Northwest A&F University, Yangling 712100, China.

China's industry is still in the middle of industrialization. Land use activities are crucial to the growth of carbon emissions. However, few scholars focus on the influence mechanism between industrial land use efficiency (ILUE) and industrial carbon emissions. In this paper, the threshold model and the spatial Durbin model are used to investigate the spatial threshold effect of industrial land use efficiency on industrial carbon emission from 2003 to 2018. The results show that ILUE of China's provinces basically shows an improvement trend, with little difference in spatial distribution, showing a pattern of high in the eastern region and low in the western region. When economic development level (A) and technical level (T) are taken as the threshold variable, ILUE has a single threshold effect on industrial carbon emissions in the eastern region. In the central region, with a as the threshold variable, ILUE shows a double threshold effect on industrial carbon emission. Under the 0-1 geographical proximity weight matrix, the indirect spillover effect of ILUE on reducing regional carbon emissions is significant, and the indirect effect is even greater than that on regional carbon emissions. The spatial spillover effect is not significant in the eastern region. These findings have important practical significance for promoting regional industrial transformation and upgrading, optimizing land space and realizing high-quality economic development.
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http://dx.doi.org/10.3390/ijerph18179368DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8430597PMC
September 2021

Structural basis for diguanylate cyclase activation by its binding partner in .

Elife 2021 Sep 9;10. Epub 2021 Sep 9.

Northwest University, Xi'an, China.

Cyclic-di-guanosine monophosphate (c-di-GMP) is an important effector associated with acute-chronic infection transition in . Previously, we reported a signaling network SiaABCD which regulates biofilm formation by modulating c-di-GMP level. However, the mechanism for SiaD activation by SiaC remains elusive. Here we determine the crystal structure of SiaC-SiaD-GpCpp complex and revealed a unique mirror symmetric conformation: two SiaD form a dimer with long stalk domains, while four SiaC bind to the conserved motifs on the stalks of SiaD and stabilize the conformation for further enzymatic catalysis. Furthermore, SiaD alone exhibits an inactive pentamer conformation in solution, demonstrating that SiaC activates SiaD through a dynamic mechanism of promoting the formation of active SiaD dimers. Mutagenesis assay confirmed that the stalks of SiaD are necessary for its activation. Together, we reveal a novel mechanism for DGC activation, which clarifies the regulatory networks of c-di-GMP signaling.
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http://dx.doi.org/10.7554/eLife.67289DOI Listing
September 2021

Learning Asymmetric and Local Features in Multi-Dimensional Data through Wavelets with Recursive Partitioning.

Authors:
Meng Li Li Ma

IEEE Trans Pattern Anal Mach Intell 2021 Sep 8;PP. Epub 2021 Sep 8.

Effective learning of asymmetric and local features in images and other data observed on multi-dimensional grids is a challenging objective critical for a wide range of image processing applications involving biomedical and natural images. It requires methods that are sensitive to local details while fast enough to handle massive numbers of images of ever increasing sizes. We introduce a probabilistic model-based framework that achieves these objectives by incorporating adaptivity into discrete wavelet transforms (DWT) through Bayesian hierarchical modeling, thereby allowing wavelet bases to adapt to the geometric structure of the data while maintaining the high computational scalability of wavelet methods---linear in the sample size (e.g., the resolution of an image). We derive a recursive representation of the Bayesian posterior model which leads to an exact message passing algorithm to complete learning and inference. While our framework is applicable to a range of problems including multi-dimensional signal processing, compression, and structural learning, we illustrate its work and evaluate its performance in the context of image reconstruction using real images from the ImageNet database, two widely used benchmark datasets, and a dataset from retinal optical coherence tomography and compare its performance to state-of-the-art methods based on basis transforms and deep learning.
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http://dx.doi.org/10.1109/TPAMI.2021.3110403DOI Listing
September 2021

Artificial Intelligence Designer for Highly-Efficient Organic Photovoltaic Materials.

J Phys Chem Lett 2021 Sep 8;12(36):8847-8854. Epub 2021 Sep 8.

MOE Key Laboratory of Low-grade Energy Utilization Technologies and Systems, School of Energy and Power Engineering, Chongqing University, 174 Shazhengjie, Shapingba, Chongqing 400044, China.

Designing efficient organic photovoltaic (OPV) materials purposefully is still challenging and time-consuming. It is of paramount importance in material development to identify basic functional units that play the key roles in material performance and subsequently establish the substructure-property relationship. Herein, we describe an automatic design framework based on an in-house designed La FREMD Fingerprint and machine learning (ML) algorithms for highly efficient OPV donor molecules. The key building blocks are identified, and a library consisting of 18 960 new molecules is generated within this framework. Through investigating the chemical structures of materials with different performance, a guidance on designing efficient OPV materials is proposed. Furthermore, the most promising candidates exhibit a predicted power conversion efficiency (PCE) value of over 15% when combined with acceptor Y6. Density functional theory (DFT) studies show these candidate materials possess exceptional potential for efficient charge carrier transport. The proposed framework demonstrates the ability to design new materials based on the substructure-property relationship built by ML, which provides an alternative methodology for applying ML in new material discovery.
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http://dx.doi.org/10.1021/acs.jpclett.1c02554DOI Listing
September 2021

Association of personal fine particulate matter and its respiratory tract depositions with blood pressure in children: From two panel studies.

J Hazard Mater 2021 08 18;416:126120. Epub 2021 May 18.

Department of Occupational and Environmental Health, Ministry of Education Key Laboratory of Environment and Health, and State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China. Electronic address:

Evidence is limited regarding the acute effects of personal fine particulate matter (PM) exposure and its respiratory tract depositions on the alteration of children's blood pressure (BP). We conducted 2 longitudinal panel studies in 2 cities to evaluate the relations of 72-h real-time personal PM exposure and its depositions in 3 respiratory tract regions over different lag times with BP and the risk of prehypertension and hypertension among 286 children aged 4-12 years. We found the strongest effects of PM exposure on increased BP and risk of prehypertension and hypertension at lag 2 day, in dose-response manner, even when PM below Chinese Ambient Air Quality Standard (CAAQS) Grade II. Moreover, compared to PM, tracheobronchial and alveolar depositions displayed more evident effects on BP outcomes. Interestingly, all above relationships were stronger among children in Guangzhou with lower PM and its deposited doses than those in Weinan. Additionally, boys and those with daily extra-school activity ≥ 1 h were more susceptible to PM-induced BP effects with significant interactions. Our results highlighted that short-term PM exposure and its respiratory tract depositions were dose-responsive related to higher BP, prevalence of prehypertension and hypertension among children, even when PM below CAAQS II.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126120DOI Listing
August 2021

Formation of viable but nonculturable state of Staphylococcus aureus under frozen condition and its characteristics.

Int J Food Microbiol 2021 Aug 27;357:109381. Epub 2021 Aug 27.

College of Food Science and Engineering, Jilin University, 5333 Xi'an Road, Changchun 130062, PR China. Electronic address:

Viable but nonculturable (VBNC) state of microorganisms has attracted much attention due to its characteristics, including the difficulty in detection by culture-based methods, virulence retention, high resistance, and so on. As a foodborne pathogen, Staphylococcus aureus is widely distributed, and has been found to enter the VBNC state under some environmental stresses, posing a potential threat to human health. Freezing is a common condition for food storage. This study investigated whether citric acid, a common food additive, could induce S. aureus into the VBNC state at -20 °C. By measuring the number of culturable and viable cells, it was found that S. aureus entered the VBNC state after 72 days of induction in citric acid buffer at -20 °C. The VBNC cells were then successfully resuscitated at 37 °C in trypsin soybean medium (TSB) with or without heat shock treatment, and TSB supplemented with sodium pyruvate and Tween 80 after 48 h. Heat shock resulted in an excellent resuscitation effect. Observed by transmission electron microscopy, the internal structure of VBNC cells was found markedly changed, compared with that of exponential phase cells. API ZYM kit was used to compare the intracellular enzyme activity of S. aureus in the exponential phase with that in the VBNC state. The results showed that the enzyme activity decreased significantly in VBNC cells, and that the VBNC cells were more resistant to simulated gastrointestinal fluid through flow cytometry analysis. Quantitative reverse-transcription polymerase chain reaction results suggested that the ability of adhesion and biofilm formation of VBNC cells might be decreased due to the down-regulation of related genes. However, it should not be ignored the recovery potential of biofilm-forming ability of VBNC cells caused by the high expression of sarA. In conclusion, S. aureus could be induced into the VBNC state in citric acid buffer at -20 °C, which showed changes in some biological characteristics and could resuscitate successfully by many conditions. Food industry needs to pay attention to the potential hazard by VBNC S. aureus under frozen conditions.
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http://dx.doi.org/10.1016/j.ijfoodmicro.2021.109381DOI Listing
August 2021

Chiral TADF-Active Polymers for High-Efficiency Circularly Polarized Organic Light-Emitting Diodes.

Angew Chem Int Ed Engl 2021 Sep 6. Epub 2021 Sep 6.

Institute of Chemistry Chinese Academy of Sciences, CAS Key Laboratory of Molecular Recognition and Function, 100190, Beijing, CHINA.

A strategy of chiral donor-acceptor copolymerization is proposed to develop chiral nonconjugated polymers with thermally activated delayed fluorescence (TADF). Based on this strategy, two pairs of chiral polymers ( R , R )-/( S , S )- pTpAcDPS and ( R , R )-/( S , S )- pTpAcBP were synthesized. The alternating copolymerization of the chiral donors and acceptors could effectively separate the frontier molecular orbitals, which made the polymers show small Δ E ST of 0.01-0.03 eV and efficient TADF properties. Moreover, the polymers also showed the quantum yield of up to 92% and the circularly polarized luminescence. The solution-processed circularly polarized organic light-emitting diodes showed circularly polarized electroluminescence signals with high external quantum efficiencies of up to 22.1% and maximum luminance of up to 34350 cd m -2 . This is the first report of CP-OLEDs based on chiral TADF polymer, which provides a useful and valuable guidance for the development of high-efficiency CPEL polymers.
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http://dx.doi.org/10.1002/anie.202110794DOI Listing
September 2021

Inhibition of Rescues Myogenesis Defects in Myotonic Dystrophy Type 1 Myoblast Model.

Front Cell Dev Biol 2021 19;9:710112. Epub 2021 Aug 19.

Anhui Provincial Key Laboratory of Molecular Enzymology and Mechanism of Major Diseases, College of Life Sciences, Anhui Normal University, Wuhu, China.

Myotonic dystrophy type 1 (DM1) is an inherited neuromuscular disease caused by expanded CTG repeats in the 3' untranslated region (3'UTR) of the gene. The myogenesis process is defective in DM1, which is closely associated with progressive muscle weakness and wasting. Despite many proposed explanations for the myogenesis defects in DM1, the underlying mechanism and the involvement of the extracellular microenvironment remained unknown. Here, we constructed a DM1 myoblast cell model and reproduced the myogenesis defects. By RNA sequencing (RNA-seq), we discovered that periostin () was the most significantly upregulated gene in DM1 myogenesis compared with normal controls. This difference in was confirmed by real-time quantitative PCR (RT-qPCR) and western blotting. Moreover, was found to be significantly upregulated in skeletal muscle and myoblasts of DM1 patients. Next, we knocked down using a short hairpin RNA (shRNA) in DM1 myoblast cells and found that the myogenesis defects in the DM1 group were successfully rescued, as evidenced by increases in the myotube area, the fusion index, and the expression of myogenesis regulatory genes. Similarly, knockdown in normal myoblast cells enhanced myogenesis. As POSTN is a secreted protein, we treated the DM1 myoblast cells with a POSTN-neutralizing antibody and found that DM1 myogenesis defects were successfully rescued by POSTN neutralization. We also tested the myogenic ability of myoblasts in the skeletal muscle injury mouse model and found that knockdown improved the myogenic ability of DM1 myoblasts. The activity of the TGF-β/Smad3 pathway was upregulated during DM1 myogenesis but repressed when inhibiting with a shRNA or a POSTN-neutralizing antibody, which suggested that the TGF-β/Smad3 pathway might mediate the function of in DM1 myogenesis. These results suggest that is a potential therapeutical target for the treatment of myogenesis defects in DM1.
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http://dx.doi.org/10.3389/fcell.2021.710112DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8417118PMC
August 2021

Comparative genomic analysis reveals metabolic flexibility of Woesearchaeota.

Nat Commun 2021 Sep 6;12(1):5281. Epub 2021 Sep 6.

Shenzhen Key Laboratory of Marine Microbiome Engineering, Institute for Advanced Study, Shenzhen University, Shenzhen, China.

The archaeal phylum Woesearchaeota, within the DPANN superphylum, includes phylogenetically diverse microorganisms that inhabit various environments. Their biology is poorly understood due to the lack of cultured isolates. Here, we analyze datasets of Woesearchaeota 16S rRNA gene sequences and metagenome-assembled genomes to infer global distribution patterns, ecological preferences and metabolic capabilities. Phylogenomic analyses indicate that the phylum can be classified into ten subgroups, termed A-J. While a symbiotic lifestyle is predicted for most, some members of subgroup J might be host-independent. The genomes of several Woesearchaeota, including subgroup J, encode putative [FeFe] hydrogenases (known to be important for fermentation in other organisms), suggesting that these archaea might be anaerobic fermentative heterotrophs.
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http://dx.doi.org/10.1038/s41467-021-25565-9DOI Listing
September 2021

GDF15 induces immunosuppression via CD48 on regulatory T cells in hepatocellular carcinoma.

J Immunother Cancer 2021 Sep;9(9)

State Key Laboratory of Cancer Biology, Fourth Military Medical University, Xi'an, Shaanxi, China

Background: A better understanding of the molecular mechanisms that manifest in the immunosuppressive tumor microenvironment (TME) is crucial for developing more efficacious immunotherapies for hepatocellular carcinoma (HCC), which has a poor response to current immunotherapies. Regulatory T (Treg) cells are key mediators of HCC-associated immunosuppression. We investigated the selective mechanism exploited by HCC that lead to Treg cells expansion and to find more efficacious immunotherapies.

Methods: We used matched tumor tissues and blood samples from 150 patients with HCC to identify key factors of Treg cells expansion. We used mass cytometry (CyTOF) and orthotopic cancer mouse models to analyze overall immunological changes after growth differentiation factor 15 (GDF15) gene ablation in HCC. We used flow cytometry, coimmunoprecipitation, RNA sequencing, mass spectrum, chromatin immunoprecipitation and , OT-I and GFP transgenic mice to demonstrate the effects of GDF15 on Treg cells and related molecular mechanism. We used hybridoma technology to generate monoclonal antibody to block GDF15 and evaluate its effects on HCC-associated immunosuppression.

Results: GDF15 is positively associated with the elevation of Treg cell frequencies in patients wih HCC. Gene ablation of GDF15 in HCC can convert an immunosuppressive TME to an inflammatory state. GDF15 promotes the generation of peripherally derived inducible Treg (iTreg) cells and enhances the suppressive function of natural Treg (nTreg) cells by interacting with a previously unrecognized receptor CD48 on T cells and thus downregulates STUB1, an E3 ligase that mediates forkhead box P3 (FOXP3) protein degradation. GDF15 neutralizing antibody effectively eradicates HCC and augments the antitumor immunity in mouse.

Conclusions: Our results reveal the generation and function enhancement of Treg cells induced by GDF15 is a new mechanism for HCC-related immunosuppression. CD48 is the first discovered receptor of GDF15 in the immune system which provide the possibility to solve the molecular mechanism of the immunomodulatory function of GDF15. The therapeutic GDF15 blockade achieves HCC clearance without obvious adverse events.
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http://dx.doi.org/10.1136/jitc-2021-002787DOI Listing
September 2021

Brachial plexus injury after clavicle fracture operation: a case report and literature review.

BMC Surg 2021 Sep 6;21(1):337. Epub 2021 Sep 6.

Department of Orthopedics, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710061, China.

Background: Open reduction and internal fixation (ORIF) is the preferred choice for treating clavicle fractures. The brachial plexus injury caused by ORIF of a clavicle fracture is very rare. If it is not treated in time, the function of the brachial plexus will be challenging to recover, which will eventually lead to upper limb dysfunction and seriously affect the patient's quality of life. Our team recently used ORIF to treat a patient with a clavicle fracture, who developed brachial plexus injury symptoms after surgery.

Case Presentation: A 34-year-old female patient was admitted to the hospital for 13 h due to the right shoulder movement restriction after a fall. Due to the significant displacement of the fracture, we used ORIF to treat the fracture. The surgery went well. When the anaesthesia effect subsided 12 h after the operation, the patient developed right brachial plexus injury symptoms, decreased right upper limb muscle strength, dysfunction, and hypoesthesia. Symptomatic treatments, such as nourishing nerve and electrical stimulation, were given immediately. Sixty days after the operation, the patient's brachial plexus injury symptoms disappeared, and the function of the right upper limb returned to the preoperative state.

Conclusions: Patients with clavicle fractures usually need to undergo a careful physical examination before surgery to determine whether symptoms of brachial plexus injury have occurred. Anaesthesia puncture requires ultrasound guidance to avoid direct damage to the brachial plexus. When the fracture end is sharp, reset should be careful to prevent nerve stump stabbed. When using an electric drill to drill holes, a depth limiter should be installed in advance to prevent the drill from damaging the subclavian nerve and blood vessels. When measuring the screw depth, the measuring instrument should be close to the bone surface and sink slowly to avoid intense hooks and damage to the brachial plexus. Try to avoid unipolar electrosurgical units to prevent heat conduction from damaging nerves, and bipolar electrocoagulation should be used instead. If symptoms of brachial plexus injury occur after surgery, initial symptomatic treatment is drugs and functional exercise, and if necessary, perform surgical exploration.
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http://dx.doi.org/10.1186/s12893-021-01335-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8422607PMC
September 2021

[New deafness gene: Progress of research on ABCC1 in biological barriers].

Zhonghua Yi Xue Yi Chuan Xue Za Zhi 2021 Sep;38(9):907-911

Department of Ear, Nose, Throat, Head and Neck Surgery, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.

ABCC1 gene is expressed in various tissues and organs of the human body, and can transport substrates including drugs, heavy metals, toxic substances and organic anions. Previous research on ABCC1 gene has mostly focused on tumor multidrug resistance. Recently, ABCC1 has been proposed as a candidate gene for hereditary hearing impairment, which has attracted much attention. ABCC1-associated deafness may be related to its role in biological barriers. This article has summarized recent progress in the study of the role of ABCC1 in the blood-testis barrier, placental barrier, blood-brain barrier, blood-labyrinth barrier, which may provide insight into its biological functions.
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http://dx.doi.org/10.3760/cma.j.cn511374-20200519-00356DOI Listing
September 2021

A bioinformatics analysis of the contribution of m6A methylation to the occurrence of diabetes mellitus.

Endocr Connect 2021 Sep 1. Epub 2021 Sep 1.

J Wang, Department of Radiology, The Second Hospital Affiliated to Kunming Medical University, Kunming, China.

N6-methyladenosine (m6A) methylation has been reported to play a role in type 2 diabetes (T2D). However, the key component of m6A methylation has not been well explored in T2D. This study investigates the biological role and underlying mechanism of m6A methylation genes in T2D. The Gene Expression Omnibus (GEO) database combined with the m6A methylation and transcriptome data of T2D patients were used to identify m6A methylation differentially expressed genes (mMDEGs). Ingenuity Pathway Analysis was used to predict T2D-related differentially expressed genes (DEGs). Gene Ontology (GO) term enrichment and the Kyoto Encyclopedia of Genes and Genomes (KEGG) were used to determine the biological functions of mMDEGs. Gene Set Enrichment Analysis (GSEA) was performed to further confirm the functional enrichment of mMDEGs and determine candidate hub genes. The Least Absolute Shrinkage and Selection Operator (LASSO) regression analysis was carried out to screen for the best predictors of T2D, and real-time polymerase chain reaction and western blot were used to verify the expression of the predictors. A total of 194 overlapping mMDEGs were detected. GO, KEGG, and GSEA analysis showed that mMDEGs were enriched in T2D and insulin signaling pathways, where the insulin gene (INS), the type 2 membranal glycoprotein gene MAFA, and hexokinase 2 (HK2) gene were found. The LASSO regression analysis of candidate hub genes showed the INS gene could be invoked as a predictive hub gene for T2D. INS, MAFA, and HK2 genes participate in the T2D disease process, but INS can better predict the occurrence of T2D.
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http://dx.doi.org/10.1530/EC-21-0328DOI Listing
September 2021

Frailty Status Among the Elderly of Different Genders and the Death Risk: A Follow-Up Study.

Front Med (Lausanne) 2021 13;8:715659. Epub 2021 Aug 13.

Beijing Institute of Geriatrics, Beijing Hospital, National Center of Gerontology, National Health Commission, Chinese Academy of Medical Sciences, Beijing, China.

Frailty in the elderly population is currently a frontier and focus in the field of health and aging. The goal of this study was to explore the frailty status among the elderly of different genders and its influence on the risk of death during 11 years. Frailty index (FI) was used to evaluate the frailty status in the elderly based on the baseline data conducted in 2009; and death as outcome variables collected in 2020 were analyzed. The difference of the frailty level and mortality of different genders was compared. Cox regression and Kaplan-Meier curves were applied to evaluate the influence on the risk of death and the 11-year survival of the elderly at different level of frailty, respectively. Totally, 1,246 elderly people were recruited. The mortality in men (43.7%, 227/519) was statistically higher than that in women (34.3%, 249/727) ( = 11.546, = 0.001). Deficits accumulated exponentially with age, and at all ages, women accumulated more deficits than do men on average ( = 0.030 vs. 0.028, = 4.137, = 0.023). For any given level of frailty, the mortality rate is higher in men than in women, and the difference in mortality between genders reached the peak when FI value was 0.26. Cox regression analysis showed that FI value had a greater impact on the risk of death in older men ( = 1.171, 95%: 1.139~1.249)than that in older women ( = 1.119, 95%: 1.039~1.137). Survival analysis showed that the median 11-year survival time in women was longer than that in men (95.26 vs. 89.52 months, = 9.249, = 0.002). Kaplan-Meier curves showed that the survival rate decreased with the increase of frailty, and at the same level of frailty, survival time in older women was longer than that in older men, except for severe frailty (FI ≥ 0.5). The frailty status and its influence on mortality are different among the older people of different genders; therefore, specific interventions for frailty should be conducted in the elderly population of different genders, as well as of different degrees of frailty.
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http://dx.doi.org/10.3389/fmed.2021.715659DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8414880PMC
August 2021

Influence of balloon location during proximal optimization technique (POT): A finite element analysis.

J Biomech 2021 Aug 20;127:110703. Epub 2021 Aug 20.

Beijing Anzhen Hospital, Capital Medical University, No.2 Anzhen Road, Chaoyang District, Beijing, China. Electronic address:

Proximal Optimization Technique (POT)is a post-expansion technique that must be completed after single-stent implantation for the coronary bifurcation. The optimal location for the distal balloon shoulder during POT remains debatable. In the present study, the finite element method is applied to simulate POT after single-stent implantation in the coronary bifurcation. Three different balloon locations based on the distal shoulder relative to the carina cut plane were analyzed: 1) "proximal":1mm before carina cut plane; 2) "standard": at the carina cut plane; and 3) "distal": 1 mm after the carina cut plane. The computational results showed differences in stent, vessel morphology, and vessel wall stress due to the different balloon locations. However, when distal balloon shoulder was located between two adjacent stent rings, it formed the distal cell of the stent, the best stent apposition, least stent structs obstruction at SB ostial. Moreover, best opening effect of distal cell of the stent can be achieved, with the least damage to the vessel wall.
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http://dx.doi.org/10.1016/j.jbiomech.2021.110703DOI Listing
August 2021

Construction of ceRNA regulatory network in mice with Echinococcosis-induced allergic reactions.

Acta Trop 2021 Sep 2;224:106120. Epub 2021 Sep 2.

Department of Anesthesiology, The First Affiliated Hospital of Xinjiang Medical University, Xinshi district, Urumqi 830054, China. Electronic address:

Background: Cystic echinococcosis (CE) is a common chronic zoonotic parasitic disease infected with the Echinococcus granulosus. This study aimed to construct the competitive endogenous RNA (ceRNA) network and investigate the mechanism of mice in echinococcosis sensitization.

Methods: The animal model of echinococcosis was established in mice, and the RNA sequencing was then performed using cysts. The differentially expressed RNAs (DERNAs: DEmRNAs, DEmiRNAs, and DElncRNAs) were screened between anaphylactic mice or non-anaphylactic mice and controls, respectively. The interactions of two DERNAs groups were identified and the ceRNA network was constructed. Moreover, the potential biological functions and pathways of the DEmRNAs were explored by enrichment analyses. Finally, the qRT-PCR and the western blot were performed to validate the expression levels of key RNAs and proteins.

Results: A total of 285 common DEmRNAs, 157 common DElncRNAs and 4 common DEmiRNAs were observed. CeRNA network contained 3 DElncRNAs, 4 DEmiRNAs, and 27 DEmRNAs corporately. Enrichment results revealed that the functions of DEmRNAs focus on biological functions and pathways that specifically interact with the immune inflammatory response. In addition, the expression of 1700099I09Rik, let-7a-5p, Ccl28 and IL-13 was validated by RT-qPCR and western blot.

Conclusion: In this study, ceRNA network associated with CE sensitization in mice was constructed. The DEmRNAs in this network may be key clues for the immune mechanism of CE sensitization.
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http://dx.doi.org/10.1016/j.actatropica.2021.106120DOI Listing
September 2021

A Comparison of Two Monoterpenoid Synthases Reveals Molecular Mechanisms Associated With the Difference of Bioactive Monoterpenoids Between and .

Front Plant Sci 2021 12;12:695551. Epub 2021 Aug 12.

Key Laboratory of Chinese Medicinal Resource from Lingnan (Ministry of Education), Guangzhou University of Chinese Medicine, Guangzhou, China.

The fruits of and are both used medicinally as the famous traditional Chinese medicine, however, the medicinal quality of is better than that of . Volatile terpenoids in the seeds, especially bornyl acetate and borneol, are the medicinal components of The volatile terpenoids and transcriptome of developing seeds of and were compared in this study. The result revealed that the bornyl acetate and borneol contents were higher in than in . Additionally, six terpenoid synthase genes () were screened from the transcriptome of , and and were found to share 98 and 83% identity with and (bornyl diphosphate synthase) from , respectively. BPPS is the key enzyme for the biosynthesis of borneol and bornyl acetate. Biochemical assays improved that AlTPS2 had an identical function to AvTPS2 as linalool synthase; however, AlTPS3 produced camphene as the major product and bornyl diphosphate (BPP) as the secondary product, whereas AvBPPS produced BPP as its major product. There was only one different amino acid between AlTPS3 (A496) and AvBPPS (G495) in their conserved motifs, and the site-directed mutation of A496G in DTE motif of AlTPS3 changed the major product from camphene to BPP. Molecular docking suggests that A496G mutation narrows the camphene-binding pocket and decreases the BPP-binding energy, thus increases the product BPP selectivity of enzyme. In addition, the expression level of was significantly higher than that of in seeds, which was consistent with the related-metabolites contents. This study provides insight into the TPS-related molecular bases for the biosynthesis and accumulation differences of the bioactive terpenoids between and . , the key gene involved in the biosynthesis of the active compound, was identified as a target gene that could be applied for the quality-related identification and breeding of .
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http://dx.doi.org/10.3389/fpls.2021.695551DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8406774PMC
August 2021

Increased incidence of human papillomavirus-related precancer or second malignancy among allogeneic stem cell transplantation patients: a SEER-Medicare population study: HPV-related precancer or second malignancy in allogeneic stem cell transplant patients.

Transplant Cell Ther 2021 Aug 30. Epub 2021 Aug 30.

Department of General Internal Medicine, The University of Texas MD Anderson Cancer Center, Houston, Texas.

Introduction: Each year more than 8,000 allogeneic stem cell transplantations (allo-SCT) are performed in the United States and approximately 30% of these patients are ≥60 years old. Allo-SCT cases have increased risk to develop human papillomavirus (HPV)-related precancer or second malignancy. It is important to evaluate HPV-related precancer or second malignancy among allo-SCT cases to develop or enhance screening and preventive practice guidelines to improve patients' survival and quality of life.

Objective: We estimated the cumulative incidence of HPV-related precancer or second malignancy in both male and female Medicare beneficiaries who received allo-SCT and compared it with non-SCT controls and non-cancer controls.

Materials And Methods: This is a retrospective matched case control study. Hematologic cancer patients aged ≥18 years who received allo-SCT between 2002 and 2011 were matched 1:5 to non-SCT controls and to non-cancer controls by age, sex, race/ethnicity, and follow-up time. Proportions of HPV-related precancer or second malignancy were estimated and compared between cases and controls using Chi-square test and logistic regression. Kaplan-Meier cumulative incidences were estimated and compared using log rank tests.

Results: We identified 700 allo-SCT cases (median age of 64 years and median follow-up time post-transplant of 4.3 years) matched with 3159 non-SCT controls and 3302 non-cancer controls. About 3.7% of allo-SCT cases developed HPV-related precancer or second malignancy post-transplant, compared with 1.9% in the non-SCT controls and 1.1% in the non-cancer controls. The odds ratio of developing HPV-related precancer or second malignancy of allo-SCT cases compared with non-SCT controls and non-cancer controls was 2.0 (95% confidence interval [CI]: 1.25-3.18) and 3.5 (95% CI: 2.1-5.8), respectively. Both allo-SCT cases and non-SCT controls had significantly higher proportions and odds in developing HPV-related precancer or second malignancy than non-cancer controls. The 5-year cumulative incidence in allo-SCT cases was 5% compared with 2.1% in non-SCT controls and 1.2% in non-cancer controls. The cumulative incidence of HPV-related precancer or second malignancy in the allo-SCT was statistically significantly higher than either of the two matched control groups, and non-SCT controls had a higher cumulative incidence of HPV-related precancer or second malignancy than that in non-cancer controls.

Discussion: Allo-SCT cases were at increased risk of developing HPV-related precancer or second malignancy compared with non-SCT controls and non-cancer controls. Routine screening of HPV-related precancer or second malignancy in allo-SCT cases is needed to prevent HPV-related precancer or second malignancy.
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http://dx.doi.org/10.1016/j.jtct.2021.08.020DOI Listing
August 2021

The complete mitochondrial genome of (Pall.) Kuntze and its phylogenetic implications.

Mitochondrial DNA B Resour 2021 27;6(9):2757-2758. Epub 2021 Aug 27.

National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, China.

The complete mitochondrial genome sequence of (Pall.) Kuntze was assembled and characterized in the present study. The mitochondrial genome was 347,227 bp in length and had a GC content of 43.84%, including 24 transfer RNA (tRNA) genes and three ribosomal RNA (rRNA) genes. Phylogenetic analysis showed that was close to and .
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http://dx.doi.org/10.1080/23802359.2021.1967805DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8405100PMC
August 2021
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