Publications by authors named "Meng Huang"

207 Publications

A wireless spinal stimulation system for ventral activation of the rat cervical spinal cord.

Sci Rep 2021 Jul 21;11(1):14900. Epub 2021 Jul 21.

Department of Neurosurgery, Center for Neuroregeneration, Houston Methodist Research Institute, Houston, USA.

Electrical stimulation of the cervical spinal cord is gaining traction as a therapy following spinal cord injury; however, it is difficult to target the cervical motor region in a rodent using a non-penetrating stimulus compared with direct placement of intraspinal wire electrodes. Penetrating wire electrodes have been explored in rodent and pig models and, while they have proven beneficial in the injured spinal cord, the negative aspects of spinal parenchymal penetration (e.g., gliosis, neural tissue damage, and obdurate inflammation) are of concern when considering therapeutic potential. We therefore designed a novel approach for epidural stimulation of the rat spinal cord using a wireless stimulation system and ventral electrode array. Our approach allowed for preservation of mobility following surgery and was suitable for long term stimulation strategies in awake, freely functioning animals. Further, electrophysiology mapping of the ventral spinal cord revealed the ventral approach was suitable to target muscle groups of the rat forelimb and, at a single electrode lead position, different stimulation protocols could be applied to achieve unique activation patterns of the muscles of the forelimb.
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http://dx.doi.org/10.1038/s41598-021-94047-1DOI Listing
July 2021

Clinical efficacy of low-dose emetine for patients with COVID-19: a real-world study.

J BioX Res 2021 Jun 3;4(2):53-59. Epub 2020 Dec 3.

Anhui Feidong County People's Hospital.

Objective: Emetine, an isoquinoline alkaloid that is enriched at high concentrations in the lung, has shown potent in vitro activity against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The aim of this study was to better understand the effectiveness of low-dose emetine for patients with coronavirus disease 2019 (COVID-19).

Methods: In this real-world study, 63 patients with mild or common COVID-19 were recruited from Wuhan Fangcang Shelter Hospital and five COVID-19-designated hospitals in Anhui Province, China from February to March 2020. Thirty-nine patients from Wuhan Fangcang Shelter Hospital were assigned to a pragmatic randomized controlled clinical trial, and 24 patients from the 5 COVID-19-designated hospitals in Anhui Province underwent a real-world study. The medication course of emetine was less than 10 days. The main symptoms and adverse reactions of all patients were observed and recorded. The primary outcome measure was the time required for a negative SARS-CoV-2 RNA result or the negative result rate on day 10. Secondary outcomes included axillary temperature, transcutaneous oxygen saturation, and respiratory frequency recovery. The study was approved by the Ethics Committee of The First Affiliated Hospital of Anhui Medical University on February 20, 2019 (approval No. PJ2020-03-19) and was registered with the Chinese Clinical Trial Registry on February 20, 2019 (registration number: ChiCTR2000030022).

Results: The oxygen saturation values were higher in the treatment group than in the control group on the first day after enrollment for patients treated at Fangcang Shelter Hospital. The axillary body temperature, respiratory rate, and oxygen saturation among patients in Fangcang Shelter Hospital were related to the time effect but not to the intervention measures. The respiratory rate and oxygen saturation of patients in the Anhui designated hospitals were related to the intervention measures but not to the time effect. The axillary body temperature of patients in Anhui designated hospitals was related to the time effect but not to the intervention measures.

Conclusion: Our preliminary study shows that low-dose emetine combined with basic conventional antiviral drugs improves clinical symptoms in patients with mild and common COVID-19 without apparent adverse effects, suggesting that moderately increased doses of emetine may have good potential for treatment and prevention of COVID-19.
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http://dx.doi.org/10.1097/JBR.0000000000000076DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8237841PMC
June 2021

HbA mutation causing false-normal HbA1c results determined by HPLC in a patient with type 2 diabetes mellitus: A case report.

Exp Ther Med 2021 Aug 7;22(2):845. Epub 2021 Jun 7.

Department of Clinical Laboratory, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266061, P.R. China.

Glycated hemoglobin A1c (HbA1c) is a convenient measure of long-term blood glucose concentrations and it is an accepted diagnostic test for type 2 diabetes mellitus (T2DM). The present study reported on a female patient with T2DM, whose fasting blood glucose and glycated albumin levels were elevated, while the HbA1c levels were in the normal range, which was inconsistent with the patient's clinical diagnosis. In the subsequent analysis, genomic DNA was extracted from the patient's blood and the HbA genes were analyzed by Sanger sequencing. The results indicated that the patient's HbA α1/2-chain genes had no mutations, while two HbA β-chain gene mutations were present, including an HBB:c.9T>C variant and a Hb G-Coushatta variant. The HBB:c.9T>C variant is a silent mutation that has no effect on HbA1c levels when detected by ion-exchange high-performance liquid chromatography (HPLC), while the Hb G-Coushatta variant may cause a discrepancy between blood glucose control and HbA1c levels when detected by ion-exchange HPLC. These results suggested that the Hb G-Coushatta variant gave rise to the false-normal result regarding HbA1c levels when detected by ion-exchange HPLC that was inconsistent with the clinical manifestations in this patient.
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http://dx.doi.org/10.3892/etm.2021.10277DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8210281PMC
August 2021

Real-time quantitative phase imaging by single-shot dual-wavelength off-axis digital holographic microscopy.

Appl Opt 2021 May;60(15):4418-4425

A single-shot dual-wavelength digital holographic microscopy with an adjustable off-axis configuration is presented, which helps realize real-time quantitative phase imaging for living cells. With this configuration, two sets of interference fringes corresponding to their wavelengths can be flexibly recorded onto one hologram in one shot. The universal expression on the dual-wavelength hologram recorded under any wave vector orientation angles of reference beams is given. To avoid as much as possible the effect of zero-order spectrum, we can flexibly select their carry frequencies for the two wavelengths using this adjustable off-axis configuration, according to the distribution feature of object's spatial-frequency spectrum. This merit is verified by a quantitative phase imaging experiment for the microchannel of a microfluidic chip. The reconstructed phase maps of living onion epidermal cells exhibit cellular internal life activities, for the first time to the best of our knowledge, vividly displaying the progress of the nucleus, cell wall, cytoskeleton, and the substance transport in microtubules inside living cells. These imaging results demonstrate the availability and reliability of the presented method for real-time quantitative phase imaging.
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http://dx.doi.org/10.1364/AO.424666DOI Listing
May 2021

Mesenchyme homeobox 1 mediated-promotion of osteoblastic differentiation is negatively regulated by mir-3064-5p.

Differentiation 2021 Jun 10;120:19-27. Epub 2021 Jun 10.

Department of Respiratory and Critical Care Medicine, The Affiliated Hospital of Qingdao University, No. 59 Haier Road, Qingdao, 266000, Shandong, China. Electronic address:

Human mesenchymal stem cells (hMSCs) are multipotent cells that can be differentiated into different cell types including osteoblasts. Herein we aimed to assess the regulation of transcription factor mesenchyme homeobox 1 (Meox1) in the osteogenic differentiation of hMSCs and to determine the microRNA which targets on Meox1. Total RNA was extracted from the isolated ligamentum flavum tissue samples and cultured hMSCs, and the expression of Meox1 was assessed by RT-PCR and Western blot assays. Cultured hMSCs were induced towards osteoblastic differentiation, and the osteoblast phenotype was determined by alkaline phosphatase activity and alizarin red staining. The microRNA targeting on the 3'-UTR of Meox1was predicted using bioinformatics tool, and the binding was validated by luciferase and RNA pulldown assays. The osteoblastic differentiation of hMSCs was checked with the knockdown of Meox1 and microRNA inhibitors. Higher expression of Meox1, and lower expression of miR-3064-5p in ossified ligamentum flavum (OLF) tissues were identified. In addition, increased expression along with the osteoblastic differentiation of hMSCs was found. Further research revealed that Meox was a direct target of miR-3064-5p, when the former promoted the differentiation of hMSCs into osteoblasts, the latter significantly suppressed the osteogenesis. The expression of Meox1 increased gradually with the osteoblastic differentiation of hMSCs, during which miR-3064-5p decreased. Meox1 is a direct target of miR-3064-5p, and they both play important roles in the osteogenesis. These findings provide potential target for the development of therapeutic drugs for skeletal system diseases.
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http://dx.doi.org/10.1016/j.diff.2021.05.002DOI Listing
June 2021

Improved oxygen activation over metal-organic-frameworks derived and zinc-modulated [email protected] catalyst for boosting indoor gaseous formaldehyde oxidation at room temperature.

J Colloid Interface Sci 2021 May 30;601:833-842. Epub 2021 May 30.

College of Materials Science and Engineering, Sichuan University, Chengdu 610065, China; Engineering Research Center of Alternative Energy Materials & Devices, Ministry of Education, Chengdu 610065, China. Electronic address:

The indoor low-concentration formaldehyde (HCHO) removal in cobalt-based catalysts is still a "hot potato". In this work, metal-organic-frameworks (MOF)-derived and Zinc (Zn)-modulated new cobalt nanoparticles catalyst ([email protected]) was designed and prepared. The [email protected] performed outstanding elimination activities for ~1 ppm HCHO at 25 °C. In the static test condition, it achieves complete HCHO removal in 3 h at a relative humidity (RH) of ~55%. Moreover, 90.18% HCHO removal ratio is held after five recycle tests. In the dynamic test condition, it remains the characteristic to eliminate around 95.89% of HCHO within 8 h under an RH of ~55% and a gas hourly space velocity (GHSV) of ~150,000 mL·hg. Such advanced results should be ascribed to large specific surface area bringing about more cobalt active sites; and it is also because residual Zn metal affects the electronic structure of [email protected] and enhance the surface charge transfer rate, thus the activation and dissociation ability of oxygen is promoted. Besides, a short HCHO reaction path over [email protected] which was clarified by the In situ DRIFTs is also a reason for excellent catalytic performance. This work represents a crucial addition to expand the family of cobalt-based catalysts for indoor HCHO elimination.
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http://dx.doi.org/10.1016/j.jcis.2021.05.173DOI Listing
May 2021

Surface Lattice Oxygen Activation by Nitrogen-Doped Manganese Dioxide as an Effective and Longevous Catalyst for Indoor HCHO Decomposition.

ACS Appl Mater Interfaces 2021 Jun 2;13(23):26960-26970. Epub 2021 Jun 2.

College of Materials Science and Engineering, Sichuan University, Chengdu 610065, China.

Oxygen vacancy plays an important role in catalytic oxidation of formaldehyde (HCHO), but the inherent drawback of its thermodynamic instability causes the deactivation of catalysts. Hence, improving the thermodynamic stability of oxygen vacancy is a crux during HCHO oxidation. Here, a novel and simple nitrogen doping of MnO/C catalyst is designed for HCHO oxidation at room temperature. The surface lattice oxygen of MnO will be activated by nitrogen-doping, which acts as active sites for HCHO oxidation and solves the thermodynamic instability issue of oxygen vacancy. Furthermore, carbon is doped with nitrogen to promote electron transfer and accelerate the HCHO oxidation process. Therefore, the catalytic activity and stability of the catalyst can be significantly promoted, which can completely remove ∼1 ppm HCHO in the tank within 3 h, and remains highly active after 5 cycles at room temperature (RH = 55%). In addition, the excellent removal performance over the prepared catalyst is also attributed to abundant surface oxygen species, amorphous crystallinity, and low reduction temperature. In situ diffuse reflectance infrared Fourier transform spectrometry (DRIFTS) and density functional theory (DFT) calculations reveal the reaction mechanism of HCHO. This strategy provides crucial enlightenment for designing novel Mn-based catalysts for application in the HCHO oxidation field.
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http://dx.doi.org/10.1021/acsami.1c04369DOI Listing
June 2021

Mechanosensitive TRPV4 Channel-Induced Extracellular ATP Accumulation at the Acupoint Mediates Acupuncture Analgesia of Ankle Arthritis in Rats.

Life (Basel) 2021 May 31;11(6). Epub 2021 May 31.

Acupuncture and Moxibustion College, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.

(1) Background Acupuncture (AP) is a safe and effective analgesic therapy. Understanding how fine needles trigger biological signals can help us optimize needling manipulation to improve its efficiency. Adenosine accumulation in treated acupoints is a vital related event. Here, we hypothesized that extracellular ATP (eATP) mobilization preceded adenosine accumulation, which involved local activation of mechanosensitive channels, especially TRPV4 protein. (2) Methods AP was applied at the injured-side Zusanli acupoint (ST36) of acute ankle arthritis rats. Pain thresholds were assessed in injured-side hindpaws. eATP in microdialysate from the acupoints was determined by luminescence assay. (3) Results AP analgesic effect was significantly suppressed by pre-injection of GdCl or ruthenium red in ST36, the wide-spectrum inhibitors of mechanosensitive channels, or by HC067047, a specific antagonist of TRPV4 channels. Microdialysate determination revealed a needling-induced transient eATP accumulation that was significantly decreased by pre-injection of HC067047. Additionally, preventing eATP hydrolysis by pre-injection of ARL67156, a non-specific inhibitor of ecto-ATPases, led to the increase in eATP levels and the abolishment of AP analgesic effect. (4) Conclusions These observations indicate that needling-induced transient accumulation of eATP, due to the activation of mechanosensitive TRPV4 channels and the activities of ecto-ATPases, is involved in the trigger mechanism of AP analgesia.
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http://dx.doi.org/10.3390/life11060513DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8228741PMC
May 2021

Comprehensive Understandings into Complete Reconstruction of Precatalysts: Synthesis, Applications, and Characterizations.

Adv Mater 2021 May 29:e2007344. Epub 2021 May 29.

State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan, 430070, China.

Reconstruction induced by external environment (such as applied voltage bias and test electrolytes) changes catalyst component and catalytic behaviors. Investigations of complete reconstruction in energy conversion recently receive intensive attention, which promote the targeted design of top-performance materials with maximum component utilization and good stability. However, the advantages of complete reconstruction, its design strategies, and extensive applications have not achieved the profound understandings and summaries it deserves. Here, this review systematically summarizes several important advances in complete reconstruction for the first time, which includes 1) fundamental understandings of complete reconstruction, the characteristics and advantages of completely reconstructed catalysts, and their design principles, 2) types of reconstruction-involved precatalysts for oxygen evolution reaction catalysis in wide pH solution, and origins of limited reconstruction degree as well as design strategies/principles toward complete reconstruction, 3) complete reconstruction for novel material synthesis and other electrocatalysis fields, and 4) advanced in situ/operando or multiangle/level characterization techniques to capture the dynamic reconstruction processes and real catalytic contributors. Finally, the existing major challenges and unexplored/unsolved issues on studying the reconstruction chemistry are summarized, and an outlook for the further development of complete reconstruction is briefly proposed. This review will arouse the attention on complete reconstruction materials and their applications in diverse fields.
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http://dx.doi.org/10.1002/adma.202007344DOI Listing
May 2021

Bioelectrical impedance analysis versus quantitative computer tomography and anthropometry for the assessment of body composition parameters in China.

Sci Rep 2021 May 26;11(1):11076. Epub 2021 May 26.

Health Management Center, The First Affiliated Hospital of Zhengzhou University, Jianshe Road, Zhengzhou, China.

Obesity, especially abdominal obesity, is correlated to increased risk of cardiovascular morbidity and mortality. It is urgent to search a simply method to predict visceral fat area (VFA). Herein, we evaluated the correlation of waist circumference (WC) measured by anthropometry and bioelectrical impedance analysis (BIA), and VFA estimated by BIA or measured by quantitative computed tomography (QCT) in China. The mean body mass index (BMI) was 25.09 ± 3.31 kg/m and the mean age was 49.16 ± 9.19 years in 2754 subjects. VFA-BIA were significantly smaller than VFA-QCT in both BMI and age subgroups between male and female (p < 0.001). High correlation was observed for WC between BIA and manually (r = 0.874 for all, r = 0.865 for male and r = 0.806 for female) and for VFA between BIA and QCT (r = 0.512 for all). The intraclass correlation coefficient (ICC) showed the perfect agreement between BIA and manually to measure WC (ICC = 0.832 for all, 0.845 for male and 0.697 for female) and implied a good reliability for VFA between BIA and QCT with women among subgroups (ICC = 0.623 for all, ICC = 0.634 for age < 50 years and ICC = 0.432 for BMI > 24 kg/m), whereas the good reliability was lost in men (ICC = 0.174). The kappa analysis showed a moderate consistency for VFA measured by BIA and QCT (Kappa = 0.522 with age < 50 years, 0.565 with age ≥ 50 years in male; Kappa = 0.472 with age < 50 years, 0.486 with age ≥ 50 years in female). In addition, BIA to estimate VFA (r = 0.758 in male, r = 0.727 in female, P < 0.001) has a stronger correlation with VFA measured by QCT than BMI and WC according to gender categories. Furthermore, ROC analysis showed the cut-off point of VFA measured by BIA for predicting visceral obesity was: 101.90 cm, 119.96 cm and 118.83 cm and the Youden's index was 0.577, 0.577 and 0.651, respectively and the Kappa value was 0.532, 0.536 and 0.611 in unadjusted model, model 1 and model 2. In conclusion, being non-invasive and free of radiation, BIA can be used as a safe and convenient tool to estimate VFA in female; especially for monitoring the VFA of the same person, the BIA has superiority to a certain extent. However, the consistency is not most ideal between BIA and QCT. When using BIA to assess whether a person is visceral obesity, we must take into consideration age, BMI and WC. Therefore, we established a regression formula to reflect VFA-QCT by VFA-BIA, age, BMI, and WC. In addition, a more accurate formula is needed to match the CT data in China.
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http://dx.doi.org/10.1038/s41598-021-90641-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8154961PMC
May 2021

On-Chip Cyclic Voltammetry Measurements Using a Compact 1024-Electrode CMOS IC.

Anal Chem 2021 06 26;93(22):8027-8034. Epub 2021 May 26.

School of Applied & Engineering Physics, Cornell University, Ithaca, New York 14853, United States.

Complementary metal-oxide-semiconductor (CMOS) microelectrode arrays integrate amplifier arrays with on-chip electrodes, offering high-throughput platforms for electrochemical sensing with high spatial and temporal resolution. Such devices have been developed for highly parallel constant voltage amperometric detection of transmitter release from multiple cells with single-vesicle resolution. Cyclic voltammetry (CV) is an electrochemical method that applies voltage waveforms, which provides additional information about electrode properties and about the nature of analytes. A 16-channel, 64-electrode-per-channel CMOS integrated circuit (IC) fabricated in a 0.5 μm CMOS process for CV is demonstrated. Each detector consists of only 11 transistors and an integration capacitor with a unit dimension of 0.0015 mm. The device was postfabricated using Pt as the working electrode material with a shifted electrode design, which makes it possible to redefine the size and the location of working electrodes. The system incorporating cell-sized (8 μm radius) microelectrodes was validated with dopamine injection tests and CV measurements of potassium ferricyanide at a 1 V/s scanning rate. The cyclic voltammograms were in excellent agreement with theoretical predictions. The technology enables rigorous characterization of electrode performance for the application of CMOS microelectrode arrays in low-noise amperometric measurements of quantal transmitter release as well as other biosensing applications.
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http://dx.doi.org/10.1021/acs.analchem.1c01132DOI Listing
June 2021

Possible Topological Hall Effect above Room Temperature in Layered CrTe Ferromagnet.

Nano Lett 2021 May 12;21(10):4280-4286. Epub 2021 May 12.

Hefei National Laboratory for Physical Sciences at the Microscale, Department of Materials Science and Engineering, CAS Key Lab of Materials for Energy Conversion, University of Science and Technology of China, Hefei, Anhui 230026, China.

Topological Hall effect (THE) has been used as a powerful tool to unlock spin chirality in novel magnetic materials. Recent focus has been widely paid to THE and possible chiral spin textures in two-dimensional (2D) layered magnetic materials. However, the room-temperature THE has been barely reported in 2D materials, which hinders its practical applications in 2D spintronics. In this paper, we report a possible THE signal featuring antisymmetric peaks in a wide temperature window up to 320 K in CrTe, a new quasi-2D ferromagnetic material. The temperature, thickness, and magnetic field dependences of the THE lead to potential spin chirality origin that is associated with the spin canting under external magnetic fields. Our work holds promise for practical applications in future chiral spin-based vdW spintronic devices.
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http://dx.doi.org/10.1021/acs.nanolett.1c00493DOI Listing
May 2021

Brain-invasive meningiomas: molecular mechanisms and potential therapeutic options.

Brain Tumor Pathol 2021 Apr 26. Epub 2021 Apr 26.

Department of Neurosurgery, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha, 410008, Hunan, People's Republic of China.

Meningiomas are the most commonly diagnosed benign intracranial adult tumors. Subsets of meningiomas that present with extensive invasion into surrounding brain areas have high recurrence rates, resulting in difficulties for complete resection, substantially increased mortality of patients, and are therapeutically challenging for neurosurgeons. Exciting new data have provided insights into the understanding of the molecular machinery of invasion. Moreover, clinical trials for several novel approaches have been launched. Here, we will highlight the mechanisms which govern brain invasion and new promising therapeutic approaches for brain-invasive meningiomas, including pharmacological approaches targeting three major aspects of tumor cell invasion: extracellular matrix degradation, cell adhesion, and growth factors, as well as other innovative treatments such as immunotherapy, hormone therapy, Tumor Treating Fields, and biodegradable copolymers (wafers), impregnated chemotherapy. Those ongoing studies can offer more diversified possibilities of potential treatments for brain-invasive meningiomas, and help to increase the survival benefits for patients.
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http://dx.doi.org/10.1007/s10014-021-00399-xDOI Listing
April 2021

Enhanced Neuroprotective Effects of Epicatechin Gallate Encapsulated by Bovine Milk-Derived Exosomes against Parkinson's Disease through Antiapoptosis and Antimitophagy.

J Agric Food Chem 2021 May 23;69(17):5134-5143. Epub 2021 Apr 23.

School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.

Epicatechin gallate (ECG) is a main effective catechin widely existing in natural plants and food, with well-known health benefits. The present study first designed a new exosome-based delivery system for ECG and examined its neuroprotective effects on a rotenone (Rot)-induced Parkinson's disease (PD) model in vitro. Exosomes (Exo) were isolated from fresh bovine milk, and their average size was 85.15 ± 2.00 nm. ECG was encapsulated into Exo by a sonication method, and the loading efficiency of ECG-loaded exosomes (ECG-Exo) was 25.96 ± 0.45%. The neuroprotective effects of ECG-Exo were evaluated on Rot-induced SHSY5Y cells and compared with free ECG. Cell viability, cellular reactive oxygen species, apoptosis rate, and the expressions of caspase-3, Bax, Bcl-2, parkin, PINK1, and Atg5 were determined. Our results showed that Exo delivered ECG successfully into SHSY5Y cells and exhibited enhanced neuroprotective effects. ECG-Exo might inhibit SHSY5Y cell damage induced by Rot through antiapoptosis and antimitophagy.
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http://dx.doi.org/10.1021/acs.jafc.0c07658DOI Listing
May 2021

Colossal Anomalous Hall Effect in Ferromagnetic van der Waals CrTe.

ACS Nano 2021 Jun 21;15(6):9759-9763. Epub 2021 Apr 21.

Hefei National Research Center for Physical Sciences at the Microscale, International Centre for Quantum Design of Functional Materials, Department of Materials Science & Engineering, CAS Key Lab of Materials for Energy Conversion, University of Science and Technology of China, Hefei, Anhui 230026, China.

van der Waals crystals exhibit excellent material performance when exfoliated to few-atomic-layer thickness. In contrast, the van der Waals thin films more than 10 nm thick are believed to show bulk properties, in which outstanding material performance is rarely found. Here we report the largest anomalous Hall conductivity observed so far in a 170 nm van der Waals ferromagnetic 1-CrTe flake, which reaches 67,000 Ω cm. Such a colossal anomalous Hall conductivity in 1-CrTe is dominated by the extrinsic skew scattering process rather than the intrinsic Berry phase effect, as evidenced by the linear relation between the anomalous Hall conductivity and the longitudinal conductivity. Defying the dilemma of mutually exclusive large anomalous Hall angle and high electric conductivity for most ferromagnets, 1-CrTe achieves both in a thin film sample. Considering the shared physics of the anomalous Hall effect and the spin Hall effect, our finding offers a guideline for searching large spin Hall materials of high conductivity which may overcome the bottleneck of overheating in spintronics devices.
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http://dx.doi.org/10.1021/acsnano.1c00488DOI Listing
June 2021

Long non‑coding RNA BANCR promotes proliferation, invasion and migration in esophageal squamous cell carcinoma cells via the Raf/MEK/ERK signaling pathway.

Mol Med Rep 2021 06 21;23(6). Epub 2021 Apr 21.

Department of Gastroenterology and Hepatology, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China.

Esophageal squamous cell carcinoma (ESCC) is a major histological type of esophageal cancer, identified as a leading cause of tumor‑associated death worldwide. In addition, long non‑coding RNA (lncRNA) BRAF‑activated non‑coding RNA (BANCR) expression is increased in the plasma of patients with ESCC, which can be reversed by tumor resection. Thus, the aim of the present study was to investigate the underlying mechanism of BANCR in ESCC progression. The relative mRNA expression of BANCR was determined via reverse transcription‑quantitative PCR. The cell behaviors of Eca‑109 cells were detected using Cell Counting Kit‑8, colony formation, wound healing and Transwell chamber assays. Finally, the expression levels of proteins involved in the Raf/MEK/ERK signaling pathway and cell metastasis were analyzed with western blotting. The results revealed that lncRNA BANCR was highly expressed in ESCC cells compared with in normal esophageal cells. BANCR overexpression enhanced proliferation, migration and invasion of ESCC cells, and BANCR silencing exerted opposite effects. Moreover, BANCR overexpression induced activation of the Raf/MEK/ERK signaling pathway in ESCC cells. Notably, U0126, a specific MEK inhibitor, decreased MEK and ERK expression, and blocked the promotive effects of BANCR overexpression on the proliferation, migration and invasion of ESCC cells. Overall, lncRNA BANCR facilitated the proliferation, migration and invasion of ESCC cells via the Raf/MEK/ERK signaling pathway. Thus, lncRNA BANCR may be a promising target for inhibiting ESCC growth and metastasis.
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http://dx.doi.org/10.3892/mmr.2021.12104DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8097753PMC
June 2021

Targeting the ILK/YAP axis by LFG-500 blocks epithelial-mesenchymal transition and metastasis.

Acta Pharmacol Sin 2021 Apr 20. Epub 2021 Apr 20.

Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Jiangsu Center for the Collaboration and Innovation of Cancer Biotherapy, Xuzhou Medical University, Xuzhou, 221004, China.

Metastasis is the main cause of mortality in patients with cancer. Epithelial-mesenchymal transition (EMT), a crucial process in cancer metastasis, is an established target for antimetastatic drug development. LFG-500, a novel synthetic flavonoid, has been revealed as a potential antitumor agent owing to its various activities, including modulation of EMT in the inflammatory microenvironment. Here, using a transforming growth factor beta (TGF-β)-induced EMT models, we found that LFG-500 inhibited EMT-associated migration and invasion in human breast cancer, MCF-7, and lung adenocarcinoma, A549, cell lines, consistent with the observed downregulation of YAP activity. Further studies demonstrated that LGF-500-induced suppression of YAP activation was mediated by integrin-linked kinase (ILK), suggesting that the ILK/YAP axis might be feasible target for anti-EMT and antimetastatic treatments, which was verified by a correlation analysis with clinical data and tumor specimens. Hence, our data support the use of LGF-500 as an antimetastatic drug in cancer therapy and provide evidence that the ILK/YAP axis is a feasible biomarker of cancer progression and a promising target for repression of EMT and metastasis in cancer therapy.
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http://dx.doi.org/10.1038/s41401-021-00655-yDOI Listing
April 2021

Hybrid Anterior Column Realignment-Pedicle Subtraction Osteotomy for Severe Rigid Sagittal Deformity.

World Neurosurg 2021 Jul 16;151:e308-e316. Epub 2021 Apr 16.

Department of Neurosurgery, Houston Methodist Hospital Neurological Institute, Houston, Texas, USA.

Objective: Recently, a hybrid anterior column realignment-pedicle subtraction osteotomy (ACR-PSO) approach has been conceived for patients with severe rigid sagittal deformity, the clinical and radiographic outcomes of which require further investigation compared with ACR only.

Methods: A single-center, retrospective chart review identified patients undergoing a combination of hyperlordotic lateral lumbar interbody grafting (ACR) and concurrent Schwab grade 3 three-column osteotomy and propensity-matched patients undergoing ACR only in the same time frame. Anterior longitudinal ligament was directly released or partially sectioned in all patients. Chart data included demographics, Oswestry Disability Index scores, ACR and osteotomy locations, cage dimensions, fusion length, and complications. Radiographic measurements included lumbar lordosis, sagittal vertical axis, pelvic tilt (PT), and proximal junctional kyphosis.

Results: Fourteen patients were enrolled in the ACR + PSO group and 36 in the ACR-only group. Mean ages were 68.5 and 63.9 years, 64% and 67% were female, average body mass index was 27.9 and 29.2, and cardiopulmonary comorbidities were 21% and 17%, respectively. There was no difference in complications (P = 0.347). The average follow-up for the ACR + PSO and ACR-only groups were 22 and 18 months, respectively. Excluding 2 mortalities, fusion occurred in all patients. Average change in lumbar lordosis measured -40.8 ± 9.2 degrees and -19.1 ± 15.7 degrees (P = 0.0006), and PT correction measured 10.5 ± 3.4 degrees and 27.3 ± 1.6 degrees (P < 0.0001), respectively.

Conclusions: For patients with severe rigid sagittal deformity, the hybrid ACR-PSO approach offers significant restoration of lumbar lordosis compared with ACR only, with similar complications but reduced PT correction.
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http://dx.doi.org/10.1016/j.wneu.2021.04.028DOI Listing
July 2021

Gut Microbiome Alterations in Patients With Thyroid Nodules.

Front Cell Infect Microbiol 2021 12;11:643968. Epub 2021 Mar 12.

Health Management Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Thyroid nodules are found in nearly half of the adult population. Accumulating evidence suggests that the gut microbiota plays an important role in thyroid metabolism, yet the association between gut microbiota capacity, thyroid nodules, and thyroid function has not been studied comprehensively. We performed a gut microbiome genome-wide association study in 196 patients with thyroid nodules and 283 controls by using whole-genome shotgun sequencing. We found that participants with high-grade thyroid nodules have decreased number of gut microbial species and gene families compared with those with lower grade nodules and controls. There are also significant alterations in the overall microbial composition in participants with high-grade thyroid nodules. The gut microbiome in participants with high-grade thyroid nodules is characterized by greater amino acid degradation and lower butyrate production. The relative abundances of multiple butyrate producing microbes are reduced in patients with high-grade thyroid nodules and the relative abundances of L-histidine metabolism pathways are associated with thyrotropin-releasing hormone. Our study describes the gut microbiome characteristics in thyroid nodules and a gut-thyroid link and highlight specific gut microbiota as a potential therapeutic target to regulate thyroid metabolism.
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http://dx.doi.org/10.3389/fcimb.2021.643968DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8005713PMC
July 2021

Circulating Genetically Abnormal Cells Add Non-Invasive Diagnosis Value to Discriminate Lung Cancer in Patients With Pulmonary Nodules ≤10 mm.

Front Oncol 2021 11;11:638223. Epub 2021 Mar 11.

Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, China.

Background: Lung cancer screening using low-dose computed tomography (LDCT) often leads to unnecessary biopsy because of the low specificity among patients with pulmonary nodules ≤10 mm. Circulating genetically abnormal cells (CACs) can be used to discriminate lung cancer from benign lung disease. To examine the diagnostic value of CACs in detecting lung cancer for patients with malignant pulmonary nodules ≤10 mm.

Methods: In this prospective study, patients with pulmonary nodules ≤10 mm who were detected at four hospitals in China from January 2019 to January 2020 were included. CACs were detected using fluorescence hybridization. All patients were confirmed as lung cancer or benign disease by further histopathological examination. Multivariable logistic regression models were established to detect the presence of lung cancer using CACs and other associated characteristics. Receiver operating characteristic analysis was used to evaluate the performance of CACs for lung cancer diagnosis.

Results: Overall, 125 patients were included and analyzed. When the cutoff value of CACs was >2, the sensitivity and specificity for lung cancer were 70.5 and 86.4%. Male (OR = 0.330, P = 0.005), maximum solid nodule (OR = 2.362, P = 0.089), maximum nodule located in upper lobe (OR = 3.867, P = 0.001), and CACs >2 (OR = 18.525, P < 0.001) met the P < 0.10 criterion for inclusion in the multivariable models. The multivariable logistic regression model that included the dichotomized CACs (>2 ≤2) and other clinical factors (AUC = 0.907, 95% CI = 0.842-0.951) was superior to the models that only considered dichotomized CACs or other clinical factors and similar to the model with numerical CACs and other clinical factors (AUC = 0.913, 95% CI = 0.850-0.956).

Conclusion: CACs presented a significant diagnostic value in detecting lung cancer for patients with pulmonary nodules ≤10 mm.
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http://dx.doi.org/10.3389/fonc.2021.638223DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7991838PMC
March 2021

Endothelin-1/Endothelin Receptor Type A-Angiopoietins/Tie-2 Pathway in Regulating the Cross Talk Between Glomerular Endothelial Cells and Podocytes in Trichloroethylene-Induced Renal Immune Injury.

J Inflamm Res 2021 9;14:761-776. Epub 2021 Mar 9.

Department of Dermatology, First Affiliated Hospital of Anhui Medical University, Hefei, Anhui Province, People's Republic of China.

Introduction: This study aimed to investigate the mechanism in regulating the cross talk between glomerular endothelial cells and podocytes in "occupational medicamentosa-like dermatitis induced by trichloroethylene (OMLDT)" patients.

Methods: Totally 6 OMLDT patients, 18 controls, and 102 BALB/c female mice were involved in this study. Patient's serum endothelin-1 (ET-1), angiopoietin-1 (Ang-1) and angiopoietin-2 (Ang-2), blood urea nitrogen (BUN), and podocalyxin (PCX) were detected. All the mice were used to establish the trichloroethylene (TCE) sensitized mouse model. Transmission electron microscope results were used to reflect renal glomerulus injury. Protein levels were detected by Western blot. Ang-1/Ang-2 gene level was reflected by RT-PCR. Cell apoptosis level was detected by using TUNEL assay kit.

Results: We found that in OMLDT patients, ET-1, Ang-2, BUN, and PCX were highly expressed but Ang-1 was inhibited. In TCE sensitized positive mouse, the downregulation of Ang-1, pTie-2 and the upregulation of Ang-2 were mediated by ET-1/ETR but not ET-1/ETR. The promotor of apoptosis proteins was downregulated and the inhibitor of apoptosis proteins was upregulated by treating with BQ123.

Discussion: ET-1/ETR-Angs/Tie-2 pathway mediated the cross talk between glomerular endothelial cells and podocytes. BQ123 can alleviate glomerulus immune injury.
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http://dx.doi.org/10.2147/JIR.S301104DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7955787PMC
March 2021

Simulation of explosive detection based on APT-TOF for verifying nuclear warhead dismantlement.

Appl Radiat Isot 2021 May 18;171:109648. Epub 2021 Feb 18.

Center for Strategic Studies, China Academy of Engineering Physics, Beijing, 100088, China.

Authentication of the explosives in nuclear warheads is a key point for the global nuclear disarmament. In this paper, a detection method of the explosives in nuclear warheads based on API-TOF (associated α particle imaging - time of flight) is proposed. First, the basic principles and current research situation of API-TOF are introduced. Second, a numerical simulation platform of the detection method of the explosives in nuclear warheads based on API-TOF was established, and a numerical simulation study of the feasibility of this method was carried out. The research results show that, this method can detect the nuclides of C, N and O of the explosive in the nuclear warhead in 15 min, and reconstruct the carbon-oxygen number ratio and nitrogen-oxygen number ratio of the explosive accurately, and reconstruct the spatial distribution of the explosive nuclei with the explosive profile clearly discriminated in the reconstruction image. From the simulation results, the detection method of the explosive in the nuclear warhead based on API-TOF is confirmed.
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http://dx.doi.org/10.1016/j.apradiso.2021.109648DOI Listing
May 2021

Impact of surgeon and hospital factors on surgical decision-making for grade 1 degenerative lumbar spondylolisthesis: a Quality Outcomes Database analysis.

J Neurosurg Spine 2021 Feb 19:1-11. Epub 2021 Feb 19.

1Department of Neurological Surgery, University of Miami, Florida.

Objective: Surgical treatment for degenerative spondylolisthesis has been proven to be clinically challenging and cost-effective. However, there is a range of thresholds that surgeons utilize for incorporating fusion in addition to decompressive laminectomy in these cases. This study investigates these surgeon- and site-specific factors by using the Quality Outcomes Database (QOD).

Methods: The QOD was queried for all cases that had undergone surgery for grade 1 spondylolisthesis from database inception to February 2019. In addition to patient-specific covariates, surgeon-specific covariates included age, sex, race, years in practice (0-10, 11-20, 21-30, > 30 years), and fellowship training. Site-specific variables included hospital location (rural, suburban, urban), teaching versus nonteaching status, and hospital type (government, nonfederal; private, nonprofit; private, investor owned). Multivariable regression and predictor importance analyses were performed to identify predictors of the treatment performed (decompression alone vs decompression and fusion). The model was clustered by site to account for site-specific heterogeneity in treatment selection.

Results: A total of 12,322 cases were included with 1988 (16.1%) that had undergone decompression alone. On multivariable regression analysis clustered by site, adjusting for patient-level clinical covariates, no surgeon-specific factors were found to be significantly associated with the odds of selecting decompression alone as the surgery performed. However, sites located in suburban areas (OR 2.32, 95% CI 1.09-4.84, p = 0.03) were more likely to perform decompression alone (reference = urban). Sites located in rural areas had higher odds of performing decompression alone than hospitals located in urban areas, although the results were not statistically significant (OR 1.33, 95% CI 0.59-2.61, p = 0.49). Nonteaching status was independently associated with lower odds of performing decompression alone (OR 0.40, 95% CI 0.19-0.97, p = 0.04). Predictor importance analysis revealed that the most important determinants of treatment selection were dominant symptom (Wald χ2 = 34.7, accounting for 13.6% of total χ2) and concurrent diagnosis of disc herniation (Wald χ2 = 31.7, accounting for 12.4% of total χ2). Hospital teaching status was also found to be relatively important (Wald χ2 = 4.2, accounting for 1.6% of total χ2) but less important than other patient-level predictors.

Conclusions: Nonteaching centers were more likely to perform decompressive laminectomy with supplemental fusion for spondylolisthesis. Suburban hospitals were more likely to perform decompression only. Surgeon characteristics were not found to influence treatment selection after adjustment for clinical covariates. Further large database registry experience from surgeons at high-volume academic centers at which surgically and medically complex patients are treated may provide additional insight into factors associated with treatment preference for degenerative spondylolisthesis.
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http://dx.doi.org/10.3171/2020.8.SPINE201015DOI Listing
February 2021

A Biaxial Strain Sensor Using a Single MoS Grating.

Nanoscale Res Lett 2021 Feb 10;16(1):31. Epub 2021 Feb 10.

Hefei National Research Center for Physical Sciences at the Microscale, Department of Materials Science and Engineering, CAS Key Lab of Materials for Energy Conversion, University of Science and Technology of China, Hefei, 230026, Anhui, China.

In this paper, we report a new type of MoS-based grating sensor for in-plane biaxial strain gauges with a precision limit of ~ 1‰. The MoS grating is numerically simulated with different biaxial strains up to 5%. Our first-principles calculations reveal that the strain sensitivity of the MoS reflectance spectrum can be considered an additional strain sensor integrated with the grating structure, enabling the mapping of in-plane biaxial strains. Our experimental studies on a prototype MoS-grating sensor further confirm that a strain component perpendicular to the grating period can cause intensity peak shifts in the grating's first-order diffraction patterns. This work opens a new path towards the sensing of in-plane biaxial strain within a single-grating device. Our new approach is applicable for other materials that have predictable reflectance response under biaxial strains and the capacity to form a two-dimensional single-crystal layer.
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http://dx.doi.org/10.1186/s11671-021-03493-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7876191PMC
February 2021

The current state of navigation in robotic spine surgery.

Ann Transl Med 2021 Jan;9(1):86

Texas Back Institute, Plano, Texas, USA.

The advent and widespread adoption of pedicle screw instrumentation prompted the need for image guidance in spine surgery to improve accuracy and safety. Although the conventional method, fluoroscopy, is readily available and inexpensive, concerns regarding radiation exposure and the drive to provide better visual guidance spurred the development of computer-assisted navigation. Contemporaneously, a non-navigated robotic guidance platform was also introduced as a competing modality for pedicle screw placement. Although the robot could provide high precision trajectory guidance by restricting four of the six degrees of freedom (DOF), the lack of real-time depth control and high capital acquisition cost diminished its popularity, while computer-assisted navigation platforms became increasingly sophisticated and accepted. The recent integration of real-time 3D navigation with robotic platforms has resulted in a resurgence of interest in robotics in spine surgery with the recent introduction of numerous navigated robotic platforms. The currently available navigated robotic spine surgery platforms include the ROSA Spine Robot (Zimmer Biomet Robotics formerly Medtech SA, Montpellier, France), ExcelsiusGPS (Globus Medical, Inc., Audubon, PA, USA), Mazor X spine robot (Medtronic Navigation Louisville, CO; Medtronic Spine, Memphis, TN; formerly Mazor Robotics, Caesarea, Israel) and TiRobot (TINAVI Medical Technologies, Beijing, China). Here we provide an overview of these navigated spine robotic platforms, existing applications, and potential future avenues of implementation.
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http://dx.doi.org/10.21037/atm-2020-ioi-07DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7859750PMC
January 2021

Double Crush Syndrome as a Cause of Hemifacial Spasm.

Cureus 2021 Jan 3;13(1):e12448. Epub 2021 Jan 3.

Neurosurgery, Houston Methodist Hospital, Houston, USA.

Hemifacial spasm is a rare but debilitating disorder of vascular compression of the facial nerve at the root exit zone causing repetitive, uncontrolled spasm of one half of the face. Usually, compression is caused by a pulsatile artery and less often by venous. Rarely, however, is hemifacial spasm caused by simultaneous compression by two formally named blood vessels. Here, we report a case of hemifacial spasm caused by simultaneous compression of the facial nerve root exit zone by the anterior inferior cerebellar artery and the superior petrosal vein. We describe the operative technique utilized to decompress the facial nerve and discuss the consequences of venous sacrifice in this scenario.
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http://dx.doi.org/10.7759/cureus.12448DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7853293PMC
January 2021

Reconstruct fissile material mass of pit based on activation effect of explosive and neural network.

Appl Radiat Isot 2021 Apr 2;170:109626. Epub 2021 Feb 2.

Center for Strategic Studies, China Academy of Engineering Physics, Beijing, 100088, China.

Future international nuclear disarmament may involve the dismantlement of nuclear warheads. In nuclear warhead dismantlement verification, the mass information of the fissile material in the pit is an important attribute of nuclear warheads, and can be used to verify that the nuclear warheads demanded by the nuclear disarmament treaty have indeed been dismantled. In this paper, a method of reconstructing the fissile material mass of the pit based on the activation effect of the explosive and the neural network is proposed, and may be applied in the future nuclear warhead dismantlement verification. Firstly, the number and average abundance of C produced by the neutron activation reactions in the explosive inside the nuclear warhead was calculated based on the Monte Carlo numerical simulation. Secondly, it is found that the spatial distribution of the C abundances in the explosive is closely related to the fissile material mass of the pit through the numerical simulation. Then, neural networks were established to reconstruct the fissile material mass of the pit through the training. The testing results show that, the fissile material mass of the pit can be reconstructed accurately based on the activation effect of the explosive and the neural network, and the reconstruction precision is better than 10%.
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http://dx.doi.org/10.1016/j.apradiso.2021.109626DOI Listing
April 2021

Detection of circulating genetically abnormal cells using 4-color fluorescence in situ hybridization for the early detection of lung cancer.

J Cancer Res Clin Oncol 2021 Aug 6;147(8):2397-2405. Epub 2021 Feb 6.

Division of Thoracic Surgery, Zhongshan Hospital, Fudan University, Shanghai, China.

Purpose: Available biomarkers lack sensitivity for an early lung cancer. Circulating genetically abnormal cells (CACs) occur early in tumorigenesis. To determine the diagnostic value of CACs in blood detected by 4-color fluorescence in situ hybridization (FISH) for lung cancer.

Methods: This was a prospective study of patients with pulmonary nodules ≤ 30 mm detected between 10/2019 and 01/2020 at four tertiary hospitals in China. All patients underwent a pathological examination of lung nodules found by imaging and were grouped as malignant and benign. CACs were detected by 4-color FISH. Patients were divided into the training and validation cohorts. Receiver operating characteristics analysis was used to analyze the diagnosis value of CACs.

Results: A total of 205 participants were enrolled. Using a cut-off value of ≥ 3, blood CACs achieved areas under the curve (AUCs) of 0.887, 0.823, and 0.823 for lung cancer in the training and validation cohorts, and all patients, respectively. CACs had high diagnostic values across all tumor sizes and imaging lesion types. CACs were decreased after surgery (median, 4 vs. 1, P < 0.001) in the validation set. The CAC status between blood and tissues was highly consistent (kappa = 0.909, P < 0.001). The AUC of CAC (0.823) was higher than that of CEA (0.478), SCC (0.516), NSE (0.506), ProGRP (0.519), and CYFRA21-1 (0.535) (all P < 0.001).

Conclusion: CACs might have a high value for the early diagnosis of lung cancer. These findings might need to be validated in future studies. Evidence suggested homology in genetic aberrations between the CACs and the tumor cells.
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http://dx.doi.org/10.1007/s00432-021-03517-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8236478PMC
August 2021

Multifunctional Nanostructure RAP-RL Rescues Alzheimer's Cognitive Deficits through Remodeling the Neurovascular Unit.

Adv Sci (Weinh) 2021 Jan 10;8(2):2001918. Epub 2020 Dec 10.

Department of Pharmacology and Chemical Biology State Key Laboratory of Oncogenes and Related Genes Shanghai Universities Collaborative Innovation Center for Translational Medicine Shanghai Jiao Tong University School of Medicine 280 South Chongqing Road Shanghai 200025 China.

Cerebrovascular dysfunction characterized by the neurovascular unit (NVU) impairment contributes to the pathogenesis of Alzheimer's disease (AD). In this study, a cerebrovascular-targeting multifunctional lipoprotein-biomimetic nanostructure (RAP-RL) constituted with an antagonist peptide (RAP) of receptor for advanced glycation end-products (RAGE), monosialotetrahexosyl ganglioside, and apolipoprotein E3 is developed to recover the functional NVU and normalize the cerebral vasculature. RAP-RL accumulates along the cerebral microvasculature through the specific binding of RAP to RAGE, which is overexpressed on cerebral endothelial cells in AD. It effectively accelerates the clearance of perivascular A, normalizes the morphology and functions of cerebrovasculature, and restores the structural integrity and functions of NVU. RAP-RL markedly rescues the spatial learning and memory in APP/PS1 mice. Collectively, this study demonstrates the potential of the multifunctional nanostructure RAP-RL as a disease-modifying modality for AD treatment and provides the proof of concept that remodeling the functional NVU may represent a promising therapeutic approach toward effective intervention of AD.
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http://dx.doi.org/10.1002/advs.202001918DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7816710PMC
January 2021

Comparison of the NOF and NOGG guidelines for spinal radiographic examination in postmenopausal Chinese women.

Arch Osteoporos 2021 01 5;16(1). Epub 2021 Jan 5.

Hospital Infection Control Center, the Second Xiangya Hospital, Central South University, 139 Middle Renmin Road, Changsha, 410011, Hunan, China.

The aim was to compare the National Osteoporosis Foundation (NOF) and the UK National Osteoporosis Guideline Group (NOGG) guidelines for the detection of vertebral fractures in postmenopausal Chinese women. The NOF guidelines had higher accuracy than the UK guidelines for spinal radiography examination.

Purpose: To compare the National Osteoporosis Foundation (NOF) and the UK National Osteoporosis Guideline Group (NOGG) guidelines for the detection of vertebral fractures in postmenopausal Chinese women.

Methods: A cross-sectional study on 255 community-dwelling postmenopausal women was conducted in Hunan province in 2017. Demographic and clinical characteristics and risk factors were recorded through questionnaires. Height and weight were measured using standard methods, and bone mineral density (BMD) was measured with dual-energy X-ray absorptiometry. Vertebral fractures were diagnosed by radiography using semi-quantitative morphometry.

Results: The prevalence of vertebral fractures was 9.4%. Women with vertebral fractures were significantly older and shorter, had higher years-since menopause and height loss values and lower BMD and T-scores at the femoral neck and total hip, and were more likely to have a history of previous fractures. The sensitivity and specificity of the NOF guidelines were 91.7% and 39.8%, respectively, while the NOGG guidelines had lower sensitivity (87.5%) and specificity (32.5%). However, a higher percentage had indications for radiography in the 50-64 years age group according to the NOGG guidelines, while a higher percentage had indications for radiography in the ≥ 65 year group according to the NOF guidelines.

Conclusions: The NOF guidelines were better than the NOGG guidelines for spinal radiography examination in the Chinese postmenopausal women.
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http://dx.doi.org/10.1007/s11657-020-00857-7DOI Listing
January 2021