Publications by authors named "Meng Ding"

129 Publications

Surface electronic states mediate concerted electron and proton transfer at metal nanoscale interfaces for catalytic hydride reduction of -NO to -NH.

Phys Chem Chem Phys 2021 Jun 4. Epub 2021 Jun 4.

Shanghai Key Laboratory of Green Chemistry and Chemical Processes, Laboratory of Interface and Water Science, College of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200062, P. R. China. and Laboratoire de chimie, Ecole Normale Supérieure de Lyon, Institut de Chimie de Lyon, Université de Lyon, 46 Allée d'italie, Lyon cedex 07 69364, France and Shandong Provincial Key Laboratory of Chemical Energy Storage and Novel Cell Technology, School of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng, 252059, Shandong, P. R. China.

Concerted electron and proton transfer is a key step for the reversible conversion of molecular hydrogen in both heterogeneous nanocatalysis and metalloenzyme catalysis. However, its activation mechanism involving electron and proton transfer kinetics remains elusive. With the most widely used catalytic hydride reduction of 4-nitrophenol (4-NP) to 4-aminophenol (4-AP) as a model reaction, we evaluate the catalytic activity of noble metal nanoparticles (NPs) trapped in porous silica in aqueous NaBH4 solution. By virtue of a novel combination of catalyst design, reaction kinetics, isotope labeling, and multiple spectroscopic techniques, the real catalytic site for the conversion of -NO2 to -NH2 is identified to be the water-hydroxyl transition metal complex, which could further react with NaBH4 to form a new triangular configuration metal complex of H3B-water-hydroxyl with dynamic features. It yields an ensemble of surface electronic states (SESs) though space overlapping of p orbitals of one B and several O atoms (including the O atoms of 4-NP), which could act as an alternative channel for concerted electron and proton transfer. This work highlights the critical role of the conceptual SESs model in heterogeneous catalysis to tune the chemical reactivity and also sheds light on the intricate working of the [FeFe]-hydrogenases.
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http://dx.doi.org/10.1039/d1cp01792fDOI Listing
June 2021

A comparison between Qigong exercise and cycle ergometer exercise for the rehabilitation of chronic obstructive pulmonary disease: A pilot randomized controlled trial (CONSORT).

Medicine (Baltimore) 2021 May;100(21):e26010

College of Physical Education, Shandong Normal University.

Background: Chronic obstructive pulmonary disease (COPD) is a common respiratory disease that is associated with significant morbidity and mortality. Exercise training confers health benefits to people with COPD. The purpose of this study was to compare differences in the rehabilitation of COPD between Qigong exercise (QE) and aerobic exercise using a cycle ergometer (CE).

Methods: This study was a randomized single-blind controlled trial. Twenty six participants were recruited and randomized to either the Qigong group or the cycle ergometer group. Both interventions lasted 12 weeks and comprised a 30 minutes supervised training session performed twice a week, that is, 24 sessions in total. The primary outcome measure was the endurance capacity measured by the six-minute walk test (6MWT). The secondary outcome measures were the results of the St. George's Hospital Respiratory Questionnaire (SGRQ) and the COPD assessment test (CAT).

Results: Participants in the group that performed aerobic exercise using a cycle ergometer had significantly improved 6MWT (P = .005), SGRQ (P = .029), and CAT (P = .018) results. Participants in the Qigong exercise group had significant changes in 6MWT (P = .033). However, the differences in 6MWT and SGRQ were not statistically significant between the 2 groups. The changes in CAT scores before and after the intervention were significantly different between the 2 groups (P = .020). There were no reports of adverse events during the course of the trial.

Conclusions: There was no difference in the primary outcome between groups. In particular, QE and cycle ergometer exercise had similar rehabilitation effects on the improvement of the cardiopulmonary endurance and quality of life of chronic obstructive pulmonary disease patients. In addition, cycle ergometer exercise may lead to a better trend of improvement in the quality of life and can improve the severity of the clinical symptoms of chronic obstructive pulmonary disease.

Trial Registration: ChiCTR-TRC-14004404.
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http://dx.doi.org/10.1097/MD.0000000000026010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8154450PMC
May 2021

Assessment of HCMV-encoded microRNAs in plasma as potential biomarkers in pregnant women with adverse pregnancy outcomes.

Ann Transl Med 2021 Apr;9(8):638

State Key Laboratory of Pharmaceutical Biotechnology, Collaborative Innovation Center of Chemistry for Life Sciences, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, NJU Advanced Institute for Life Sciences (NAILS), School of Life Sciences, Nanjing University, Nanjing, China.

Background: Human cytomegalovirus (HCMV) is the most frequent cause of congenital infections and can lead to adverse pregnancy outcomes (APOs). HCMV encodes multiple microRNAs (miRNAs) that have been reported to be partially related to host immune responses, cell cycle regulation, viral replication, and viral latency, and can be detected in human plasma. However, the relevance for HCMV-encoded miRNAs in maternal plasma as an indicator for APOs has never been evaluated.

Methods: Expression profiles of 22 HCMV-encoded miRNAs were first measured in plasma samples from 20 pregnant women with APOs and 28 normal controls using quantitative reverse-transcription polymerase chain reaction. Next, markedly changed miRNAs were validated in another independent validation set consisting of 20 pregnant women with APOs and 27 control subjects. Markedly changed miRNAs were further assessed in the placenta tissues. HCMV DNA in peripheral blood leukocytes (PBLs) and anti-HCMV immunoglobulin M (IgM) and anti-HCMV immunoglobulin G (IgG) in plasma were also examined in both training and validation sets. Diagnostic value and risk factors were compared between APO cohorts and normal controls.

Results: Analysis of the training and validation data sets revealed that plasma concentrations of hcmv-miR-UL148D, hcmv-miR-US25-1-5p and hcmv-miR-US5-1 were significantly increased in pregnant women with APOs compared with normal controls. Hcmv-miR-US25-1-5p presented the largest area under the receiver-operating characteristic (ROC) curve (AUC) (0.735; 95% CI, 0.635-0.836), with a sensitivity of 68% and specificity of 71%. Furthermore, plasma levels of hcmv-miR-US25-1-5p and hcmv-miR-US5-1 correlated positively with APOs (P=0.029 and 0.035, respectively). Hcmv-miR-US25-1-5p in the placenta tissues were dramatically increased in APOs, and correlated with plasma hcmv-miR-US25-1-5p. Nevertheless, neither the concentration of HCMV DNA in PBLs nor the positivity rates of anti-HCMV IgM and anti-HCMV IgG in plasma showed a statistically significant correlation with APOs.

Conclusions: We identified a unique signature of HCMV-encoded miRNAs in pregnant women with APOs that may be useful as a potential noninvasive biomarker for predicting and monitoring APOs during HCMV infection.
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http://dx.doi.org/10.21037/atm-20-7354DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8106018PMC
April 2021

Hepatic SENP2 Controls Systemic Metabolism via SUMOylation-dependent Regulation of Liver-Adipose Tissue Crosstalk.

Hepatology 2021 May 2. Epub 2021 May 2.

Key Laboratory of Metabolism and Molecular Medicine of the Ministry of Education, Department of Biochemistry and Molecular Biology of School of Basic Medical Sciences, Department of Endocrinology and Metabolism of Zhongshan Hospital, Fudan University, Shanghai, 200032, China.

Background & Aims: Nonalcoholic fatty liver disease (NAFLD), characterized by aberrant triglycerides accumulation in liver, affects the metabolism remodeling of hepatic and non-hepatic tissues by secreting altered hepatokines. SUMO-specific protease 2 (SENP2) is responsible for de-SUMOylation of target protein, with broad effects on cell growth, signal transduction and developmental process. However, role of SENP2 in hepatic metabolism remains unclear.

Approach & Results: Herein, we found that SENP2 was the most dramatically increased SENPs in the fatty liver, and its level was modulated by fed/fasted conditions. To define the role of hepatic SENP2 in metabolic regulation, we generated liver-specific SENP2 knockout (Senp2-LKO) mice. Senp2-LKO mice exhibited resistance to HFD-induced hepatic steatosis and obesity. RNA-seq analysis showed that Senp2 deficiency upregulated genes involved in fatty acid oxidation and downregulated genes in lipogenesis in the liver. Additionally, ablation of hepatic SENP2 activated thermogenesis of adipose tissues. Improved energy homeostasis of both the liver and adipose tissues by SENP2 disruption prompted us to detect the hepatokines, with FGF21 identified as a key factor markedly elevated in Senp2-LKO mice that maintained metabolic homeostasis. Loss of FGF21 obviously reversed the positive effects of SENP2 deficiency on metabolism. Mechanistically, by screening transcriptional factors of FGF21, PPARα was defined as the mediator for SENP2 and FGF21. SENP2 interacted with PPARα and deSUMOylated it, thereby promoting ubiquitylation and subsequent degradation of PPARα, which in turn inhibited FGF21 expression and fatty acid oxidation. Consistently, SENP2 overexpression in liver facilitated development of metabolic disorders.

Conclusions: Our finding demonstrated key role of hepatic SENP2 in governing metabolic balance by regulating liver-adipose tissue crosstalk, linking the SUMOylation process to metabolic regulation.
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http://dx.doi.org/10.1002/hep.31881DOI Listing
May 2021

Multiple Roles of Black Raspberry Anthocyanins Protecting against Alcoholic Liver Disease.

Molecules 2021 Apr 16;26(8). Epub 2021 Apr 16.

School of Functional Food and Wine, Shenyang Pharmaceutical University, Shenyang 110016, China.

This study aimed to investigate the protective effect of black raspberry anthocyanins (BRAs) against acute and subacute alcoholic liver disease (ALD). Network analysis and docking study were carried out to understand the potential mechanism. Thereafter, the serum biochemical parameters and liver indexes were measured, the histopathological changes of the liver were analyzed in vivo. The results showed that all tested parameters were ameliorated after the administration of BRAs with alcohol. Meanwhile, there was increased protein expression of NF-κB and TGF-β in extracted livers, which was associated with hepatitis and hepatic fibrosis. Furthermore, BRAs and cyanidin---rutinoside exhibited cytotoxic effects on t-HSC/Cl-6, HepG2, and Hep3B and induced the apoptosis of HepG2 cells; downregulated the protein expression level of Bcl-2; upregulated the level of Bax; and promoted the release of cytochrome C, cleaved caspase-9, cleaved caspase-3, and cleaved PARP in HepG2 cells. In addition, the antioxidant activity of BRAs was tested, and the chemical components were analyzed by FT-ICR MS. The results proved that BRAs exert preventive effect on ALD through the antioxidant and apoptosis pathways.
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http://dx.doi.org/10.3390/molecules26082313DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8073606PMC
April 2021

SOX9 in prostate cancer is upregulated by cancer-associated fibroblasts to promote tumor progression through HGF/c-Met-FRA1 signaling.

FEBS J 2021 Mar 11. Epub 2021 Mar 11.

Department of Urology, Drum Tower Hospital, Medical School of Nanjing University, Institute of Urology, Nanjing University, China.

Transcription factor SOX9 was a biomarker for prostate cancer (Pca) with poor prognosis. Nevertheless, the regulatory mechanism underlying SOX9 upregulation still remains unclear. Several cytokines have been reported to be involved in the regulation of SOX9, suggesting that cancer-associated fibroblasts (CAFs), one of the main sources of secreted factors in the tumor microenvironment (TME), may play a role in regulating SOX9 expression. Herein, an in vitro model of paracrine interaction between primary CAFs and Pca cells was applied to investigate the molecular mechanism of SOX9 upregulation during Pca progression. The regulatory axis was validated by in vivo experiments and The Cancer Genome Atlas data. Conditional medium of CAFs (CAF-CM) upregulated the expression of SOX9, which was mutually proved to be essential for CAF-induced tumor progression. Further analysis showed that hepatocyte growth factor (HGF) secreted by CAFs was responsible for SOX9 elevation in Pca cells, via the activation of c-Met signaling. Mechanistically, HGF/c-Met signaling specifically activated MEK1/2-ERK1/2 pathway, which induced phosphorylation and upregulation of FRA1, which then transcriptionally upregulated SOX9 by binding to the promoter of SOX9 gene. Moreover, we identified that HGF/c-Met-ERK1/2-FRA1-SOX9 axis was relatively conserved between human and mouse species by validating in mouse Pca cells. Our results reveal a novel insight into the molecular mechanism that SOX9 in Pca cells is promoted by CAFs through HGF/c-Met-ERK1/2-FRA1 axis. Furthermore, SOX9 may serve as an alternative marker for the activated HGF/c-Met signaling to enroll the optimal Pca patients for HGF/c-Met inhibition treatment, since it is much more stable and easier to detect.
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http://dx.doi.org/10.1111/febs.15816DOI Listing
March 2021

The effects and acceptability of different exercise modes on glycemic control in type 2 diabetes mellitus: A protocol for systematic review and network meta-analysis.

Medicine (Baltimore) 2021 Jan;100(3):e23963

College of Physical Education, Shandong Normal University.

Introduction: Exercise has been believed to have positive effects on blood glucose control in patients with type 2 diabetes mellitus. However, few medical evidences have been found to ascertain which type of exercise has the best effect on blood glucose control in diabetes and which type of exercise is more acceptable. The purpose of this study is to compare the effects and acceptability of different exercise modes on glycemic control in type 2 diabetes patients by using systematic review and network meta-analysis.

Methods And Analysis: Relevant randomized controlled trial studies will be searched from PubMed, EMbase, CochraneCENTRAL, CNKI, VIP, and Chinese medical paper libraries. Primary outcome indicators: glycosylated hemoglobin and dropout rate of the research (number of dropouts/numbers of initially enrolled subjects). Secondary outcome measures: fasting blood glucose, body weight, total cholesterol (TC), low-density lipoprotein cholesterol (LDL), high-density lipoprotein cholesterol, triglycerides (TG), diastolic pressure, systolic pressure (SBP). Two reviewers are arranged to screen Title, Abstract, and then review full text to further extract data. Standard meta-analysis and network meta-analysis of the data are performed afterward. Methodological quality assessment is planned to be conducted using Cochrane risk of bias tool. The outcome will be analyzed statistically according to Bayesian analysis methods. After that, subgroup analysis is conducted on the duration of intervention, whether there is supervision of intervention, frequency of intervention per week, age, gender, and medication use.

Trial Registration Number: PROSPERO CRD42020175181.

Discussion: The systematic review and network meta-analysis include evidence of the impact of different exercise modes on blood glucose control in type 2 diabetes mellitus. There are 2 innovative points in this study. One is to conduct a classified study on exercise in as much detail as possible, and the other is to study the acceptability of different exercise modes. The network meta-analysis will reduce the uncertainty of intervention and enable clinicians, sports practitioners, and patients to choose more effective and suitable exercise methods.

Ethics And Dissemination: The findings of the study will be disseminated through publications in peer-reviewed journals and scientific conferences and symposia. Further, no ethical approval is required in this study.
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http://dx.doi.org/10.1097/MD.0000000000023963DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7837820PMC
January 2021

LncRNA LINC00511 Acts as an Oncogene in Colorectal Cancer via Sponging miR-29c-3p to Upregulate NFIA.

Onco Targets Ther 2020 5;13:13413-13424. Epub 2021 Jan 5.

Department of Endoscopy Center, China-Japan Union Hospital of Jilin University, Changchun 130033, Jilin, People's Republic of China.

Background: Colorectal cancer (CRC), characterized by high mortality and incidence rate, is one of the most common types of rectum tumors in the gastrointestinal tract worldwide. An increasing number of investigations indicated that long noncoding RNAs (lncRNAs) have been implicated in the growth of a wide range of cancers. Although it has obtained general acceptance that lncRNA LINC00511 plays a significant role in numerous cancers, the regulatory mechanism of LINC00511 in CRC still needs to be explored.

Materials And Methods: Bioinformatics analysis and a wide range of experiments of sphere formation assay, cell proliferation assay, RT-qPCR, colony formation assay, Transwell assay and Western blot assays investigated the function and mechanism of LINC00511 in CRC tissues and cells.

Results: Our results manifested that the expression level of LINC00511 was obviously upregulated in CRC tissues and cells and it accelerated CRC development through facilitating cell proliferation, metastasis and stemness. Molecular mechanism exploration uncovered that LINC00511 acted as a ceRNA competing with to bind with miR-29c-3p. Through rescue experiments, we discovered that NFIA upregulation partly counteracted the inhibitive effect induced by LINC00511 silencing on CRC progression.

Conclusion: These results revealed that LINC00511 participated in the progression of CRC by targeting the LINC00511/miR-29c-3p/ axis, indicating that LINC00511 may be a potential therapeutic target for CRC treatment.
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http://dx.doi.org/10.2147/OTT.S250377DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7847767PMC
January 2021

Purification of ginseng rare sapogenins 25-OH-PPT and its hypoglycemic, antiinflammatory and lipid-lowering mechanisms.

J Ginseng Res 2021 Jan 9;45(1):86-97. Epub 2019 Nov 9.

School of Functional Food and Wine, Shenyang Pharmaceutical University, Shenyang, Liaoning, China.

Background: Panax ginseng Meyer has been used as a nourishing edible herb in East Asia for thousands of years. 25-OH-PPT was first discovered as a natural rare triterpenoid saponin in ginseng stems and leaves by our group. Research found that it showed strong inhibitory effects on -glucosidase and protein tyrosine phosphatase 1B, and protected cardiocytes (H9c2) through PI3K/Akt pathway.

Methods: In the research, in order to optimize the 25-OH-PPT enrichment process, optimal macroporous resins and optimal purification conditions were studied. Meanwhile, the hypoglycemic effect and mechanism of 25-OH-PPT were evaluated by using STZ to establish insulin-dependent diabetic mice and the spontaneous type 2 diabetes DB/DB mice.

Results And Conclusion: Research found that 25-OH-PPT can reduce blood glucose and enhance glucose tolerance in STZ model mice. It increases insulin sensitivity by upregulating GLUT4 and AMPK in skeletal muscle, and activating insulin signaling pathways. In DB/DB mice, 25-OH-PPT achieves hypoglycemic effects mainly by activating the insulin signaling pathway. Meanwhile, through the influence of liver inflammatory factors and lipids in serum, it can be seen that 25-OH-PPT has obvious anti-inflammatory and lipid-lowering effects. These results provide new insights into the study of ginseng as a functional food.
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http://dx.doi.org/10.1016/j.jgr.2019.11.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7791145PMC
January 2021

B7-H3-Induced Signaling in Lung Adenocarcinoma Cell Lines with Divergent Epidermal Growth Factor Receptor Mutation Patterns.

Biomed Res Int 2020 24;2020:8824805. Epub 2020 Dec 24.

Department of Laboratory Medicine, Second Hospital of Anhui Medical University, 678 Furong Road, Hefei, Anhui 230601, China.

The cosignal molecule B7-H3 is gaining attention due to its abnormal expression and abundant signal transduction in many types of malignancies. B7-H3-induced signaling includes at least three cascades: PI3K/AKT, JAK2/STAT3, and Raf/MEK/ERK1/2, which are also involved in epidermal growth factor receptor- (EGFR-) triggered signaling in lung adenocarcinoma cells. However, the correlation between B7-H3-induced signaling and EGFR signaling, and between B7-H3-targeted immunotherapy and EGFR-targeted therapy in lung adenocarcinoma, remains to be elucidated. Herein we find that knockout of gene decreased cell survival and increased EGFR-tyrosine kinase inhibitor gefitinib susceptibility of both H3255 and HCC827 cells, two lung adenocarcinoma cell lines harboring EGFR L858R (exon 21) and Del E746-A750 (exon 19) mutations, respectively. deletion resulted in dramatic reduction of phosphorylation level of AKT and STAT3 in H3255 cells while having mild-to-moderate suppression on AKT, STAT3, and ERK1/2 in HCC827 cells. Gefitinib had similar effects with deletion both in H3255 and HCC827 cells. Furthermore, ablation had significant synergistic effects with gefitinib in HCC827 cells. Collectively, our study reveals B7-H3-induced signaling in lung adenocarcinoma cell lines with divergent EGFR mutations, and a translational potential of combined targeted therapy of B7-H3 and EGFR in lung adenocarcinoma with EGFR Del E746-A750 mutation.
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http://dx.doi.org/10.1155/2020/8824805DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7775133PMC
May 2021

Different expression pattern of human cytomegalovirus-encoded microRNAs in circulation from virus latency to reactivation.

J Transl Med 2020 12 9;18(1):469. Epub 2020 Dec 9.

Department of Clinical Laboratory, Jinling Hospital, State Key Laboratory of Analytical Chemistry for Life Science, Nanjing University School of Medicine, Nanjing University, Nanjing, 210002, China.

Background: Human cytomegalovirus (HCMV) is a beta-hersvirinae that has a high latent infection rate worldwide and can cause serious consequences in immunocompromised patients when reactivation; however, the mechanism of how HCMV convert from latent to reactivation has rarely been investigated. In the present study, we aimed to perform a comprehensive analysis of the HCMV-encoded microRNA (miRNA) profile in serum of patients upon HCMV reactivation from latency and to further evaluate its clinical significance for the disease monitoring and preventing usefulness.

Methods: Serum samples from 59 viremia patients and 60 age-gender matched controls were enrolled in this study for screening and validation of different expression of HCMV miRNAs. Serum concentrations of 22 known HCMV miRNAs were determined by a hydrolysis probe-based stem-loop quantitative reverse transcription polymerase chain reaction (RT-qPCR) assay. HCMV DNA was measured by quantitative real-time PCR (qPCR) with the whole blood sample. Serum HCMV IgG and IgM were assessed using enzyme linked immunosorbent assay (ELISA). Another 47 samples from 5 patients at different time points were collected to evaluate the monitoring effectiveness and disease prediction ability of differential expression HCMV-miRNAs during the antiviral treatment.

Results: The RT-qPCR analysis revealed that the serum levels of 16 of the 22 examined HCMV miRNAs were elevated in HCMV viremia patients compared with controls, and a profile of 8 HCMV miRNAs including hcmv-miR-US25-2-3p, hcmv-miR-US4-5p, hcmv-miR-US25-2-5p, hcmv-miR-US25-1-3p, hcmv-miR-US25-1, hcmv-miR-UL36, hcmv-miR-UL148D, hcmv-miR-US29-3p were markedly elevated (fold change > 2, P < 0.01). Receiver operating characteristic curve (ROC) analysis were performed on the selected HCMV-miRNAs in all of the patients and controls that enrolled in this study, and which ranged from 0.72 to 0.80 in the autoimmune patients. In addition, hcmv-miR-US25-1-3p levels were significantly correlated with HCMV DNA load (r = 0.349, P = 0.007), and were obviously higher in the reactivation set than the latency set in the autoimmune patients, which could be a predictor for the monitoring of the antiviral treatment.

Conclusions: HCMV miRNAs profile showed markedly shift-switch from latency to reactivation in circulation from HCMV infected patients and hcmv-miR-US25-1-3p may be served as a predictor for the switch upon reactivation from latency in patients suffered with autoimmune diseases.
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http://dx.doi.org/10.1186/s12967-020-02653-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7727131PMC
December 2020

Long noncoding RNA TMPO-AS1/miR-126-5p/BRCC3 axis accelerates gastric cancer progression and angiogenesis via activating PI3K/Akt/mTOR pathway.

J Gastroenterol Hepatol 2020 Dec 8. Epub 2020 Dec 8.

Department of Endoscopy Center, China-Japan Union Hospital of Jilin University, Changchun, China.

Background And Aim: Gastric cancer (GC) is an aggressive tumor featured by uncontrolled cell proliferation and metastasis. In recent years, long noncoding RNAs (lncRNAs) act as crucial regulators and biological markers in multiple cancers. LncRNA TMPO-AS1 has been revealed to be an oncogene in some cancers. Nevertheless, there is little known about the biological role of TMPO-AS1 in GC.

Methods: Reverse transcription-quantitative polymerase chain reaction analysis was used to examine the expression level of TMPO-AS1 in GC tissues and cells. Cell Counting Kit-8, colony formation, wound healing assays, and western blot analysis were performed to determine the role of TMPO-AS1 in GC cells. RNA pull-down, luciferase reporter, and RNA immunoprecipitation assays were used to test the interaction among TMPO-AS1, miR-126-5p, and BRCC3.

Results: TMPO-AS1 was highly expressed in GC tissues and cells. Upregulated TMPO-AS1 was closely associated with adverse prognosis of GC patients. Functional assays showed that TMPO-AS1 promoted GC cell proliferation, migration, and angiogenesis. Furthermore, it was found that TMPO-AS1 acted as a competing endogenous RNA for miR-126-5p to upregulate BRCC3 expression. Rescue assays revealed that TMPO-AS1 facilitated cellular progression of GC by sponging miR-126-5p and upregulating BRCC3. In addition, we found that the effects of the TMPO-AS1/miR-126-5p/BRCC3 axis on GC cell progression were related to the PI3K/Akt/mTOR pathway.

Conclusions: Our study demonstrated that the TMPO-AS1/miR-126-5p/BRCC3 axis was involved in GC progression via the regulation of PI3K/Akt/mTOR pathway, which might provide a potential therapeutic strategy for GC.
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http://dx.doi.org/10.1111/jgh.15362DOI Listing
December 2020

Orderly MOF-Assembled Hybrid Monolithic Stationary Phases for Nano-Flow HPLC.

Anal Chem 2020 12 30;92(24):15757-15765. Epub 2020 Nov 30.

Department of Chemistry & the MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.

We report an approach that polymerizable handle-modified nanosized metal organic frameworks (MOFs) are used as independent monomers to be covalently organized by crosslinking molecules (CLMs) into an orderly MOF-assembled hybrid monolithic stationary phase, overcoming the respective problems of previously reported MOF-mixed or embedded stationary phases so far. It has a hierarchical micro-, meso-, and macropore structure throughout the monolithic matrix that is donated from MOF themselves, formed via CLM crosslinking in-between MOFs and expended by porogenic solvents, and a tunable surface chemistry derived inherently from MOFs, regulated by CLMs and initiated by the mobile phases as well. Such a pore structure and surface chemistry display multiplex interactions of sieving and electrostatic repulsion in addition to the polarity-based interactions that synergistically govern the partitioning way and degree of target molecules between the stationary and mobile phases, thus offering the ability to simultaneously separate small and large molecules during one chromatographic run on a nano-flow capillary high-performance liquid chromatography platform. A baseline mutual separation with the HETP and of, for example, 9.2 μm butylbenzene and 4.56 (butylbenzene and pentylbenzene), 7.9 μm (phenylalanine) and 3.50 (tryptophan and phenylalanine), and 7.0 μm (myoglobin) and 1.91 (bovine serum albumin and myoglobin) was achieved when UiO-66/NH-methacrylate was exemplified as a model of MOFs and 1,6-hexanediol dimethacrylate and stearyl methacrylate together as CLMs. Not limited to the MOFs and CLMs demonstrated here, other available MOFs and CLMs or newly designed and synthesized ones are expected to be used for constructing one's own desired monolithic stationary phases toward her/his particular purposes.
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http://dx.doi.org/10.1021/acs.analchem.0c02706DOI Listing
December 2020

Relationship between Smoking, Physical Activity, Screen Time, and Quality of Life among Adolescents.

Int J Environ Res Public Health 2020 10 31;17(21). Epub 2020 Oct 31.

Department of Sport and Health, School of Physical Education, Shandong University, Jinan 250061, China.

Background: Quality of life (QOL) is a crucial part of evaluating health conditions IN adolescents. The purposes of this study were to (1) examine the relationship of QOL and smoking, physical activity (PA) and screen time (ST) among Chinese adolescents, (2) explore the relationship between PA-ST combination and QOL of adolescents, and (3) investigate the dose-response relationship between PA-ST and QOL.

Methods: This study randomly selected 12,900 adolescents (11-18 years) from 13 administrative regions in Shandong Province, China. The data gathering tools for Smoking (tobacco), PA (PAQ-A) and ST (average daily time for ST) and QOL questionnaire (child and adolescent quality of life scale) were completed among all adolescents. Statistical analysis was performed by T test, chi-square test and multiple linear regression.

Results: 12,641 adolescents (aged 12-18) completed the study. In multiple linear regression models, the result demonstrated that the adolescents from rural areas, with high ST, low PA, and smoking, with older age and low socioeconomic status, showed a lower QOL score. First-time smokers under 10 years revealed the lowest QOL, and PA > 30 min five days per week have the highest QOL. In addition, boys and girls with PA > 30 min three to four days per week in high ST group obtain the higher scores (boys β = 5.951, girls β = 3.699) than low PA-low ST groups.

Conclusions: Adolescents from rural areas suffer from a relatively poorer QOL. More than 30 min of PA five or more days for boys and three or four days per week for girls could decrease negative effects of ST and improve QOL.
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http://dx.doi.org/10.3390/ijerph17218043DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7662320PMC
October 2020

Meta-Analysis of Randomized Controlled Trials of the Effects of Tai Chi on Blood Pressure.

Evid Based Complement Alternat Med 2020 7;2020:8503047. Epub 2020 Oct 7.

Department of Sport and Health, School of Physical Education, Shandong University, Jinan 250061, China.

Objectives: The purpose of this study was to investigate the influences of Tai Chi on blood pressure (BP) using the meta-analysis.

Methods: This paper used 6 e-resource databases, and randomized controlled trials on the role of Tai Chi on blood pressure were retrieved. Besides, the meta-analysis was conducted according to the guidelines of the Moose-recommendations and applied with Review Manager 5.3, and the risk of bias assessment was performed with the Cochrane Collaboration's tool. The inclusion, data extraction, and risk of bias assessment were independently finished by two researchers.

Results: There are 24 trials meeting the criteria of inclusion and the results were reviewed. The meta-analysis indicates that, compared with no exercise, Tai Chi had the influence of lowering systolic blood pressure (mean difference = -6.07, 95%CI (-8.75, -3.39), < 0.00001) and diastolic blood pressure (mean difference MD = -3.83, 95%CI (-4.97, -2.69), < 0.00001). No significant discrepancies in all outcomes between Tai Chi and other aerobic exercises were discovered.

Conclusion: Tai Chi can significantly reduce systolic and diastolic pressure than inactivity. However, Tai Chi does not show advantages in reducing blood pressure compared to other aerobic exercises. The trial is registered with CRD42020175306.
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http://dx.doi.org/10.1155/2020/8503047DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7563036PMC
October 2020

Stem cell therapies for periodontal tissue regeneration: a network meta-analysis of preclinical studies.

Stem Cell Res Ther 2020 10 2;11(1):427. Epub 2020 Oct 2.

Department of Oral Implantology, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, China.

Background: Periodontal tissue regeneration (PTR) is the ultimate goal of periodontal therapy. Currently, stem cell therapy is considered a promising strategy for achieving PTR. However, there is still no conclusive comparison that distinguishes clear hierarchies among different kinds of stem cells.

Methods: A systematic review and network meta-analysis (NMA) was performed using MEDLINE (via PubMed), EMBASE, and Web of Science up to February 2020. Preclinical studies assessing five types of stem cells for PTR were included; the five types of stem cells included periodontal ligament-derived stem cells (PDLSCs), bone marrow-derived stem cells (BMSCs), adipose tissue-derived stem cells (ADSCs), dental pulp-derived stem cells (DPSCs), and gingival-derived stem cells (GMSCs). The primary outcomes were three histological indicators with continuous variables: newly formed alveolar bone (NB), newly formed cementum (NC), and newly formed periodontal ligament (NPDL). We performed pairwise meta-analyses using a random-effects model and then performed a random-effects NMA using a multivariate meta-analysis model.

Results: Sixty preclinical studies assessing five different stem cell-based therapies were identified. The NMA showed that in terms of NB, PDLSCs (standardized mean difference 1.87, 95% credible interval 1.24 to 2.51), BMSCs (1.88, 1.17 to 2.59), and DPSCs (1.69, 0.64 to 2.75) were statistically more efficacious than cell carriers (CCs). In addition, PDLSCs were superior to GMSCs (1.49, 0.04 to 2.94). For NC, PDLSCs (2.18, 1.48 to 2.87), BMSCs (2.11, 1.28 to 2.94), and ADSCs (1.55, 0.18 to 2.91) were superior to CCs. For NPDL, PDLSCs (1.69, 0.92 to 2.47) and BMSCs (1.41, 0.56 to 2.26) were more efficacious than CCs, and PDLSCs (1.26, 0.11 to 2.42) were superior to GMSCs. The results of treatment hierarchies also demonstrated that the two highest-ranked interventions were PDLSCs and BMSCs.

Conclusion: PDLSCs and BMSCs were the most effective and well-documented stem cells for PTR among the five kinds of stem cells evaluated in this study, and there was no statistical significance between them. To translate the stem cell therapies for PTR successfully in the clinic, future studies should utilize robust experimental designs and reports.
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http://dx.doi.org/10.1186/s13287-020-01938-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7531120PMC
October 2020

SCD1 promotes lipid mobilization in subcutaneous white adipose tissue.

J Lipid Res 2020 12 25;61(12):1589-1604. Epub 2020 Sep 25.

Key Laboratory of Metabolism and Molecular Medicine, Ministry of Education, Department of Biochemistry and Molecular Biology of the School of Basic Medical Sciences, and Department of Endocrinology and Metabolism of Zhongshan Hospital, Fudan University, Shanghai Medical College, Shanghai, China.

Beiging of white adipose tissue (WAT) has beneficial effects on metabolism. Although it is known that beige adipocytes are active in lipid catabolism and thermogenesis, how they are regulated deserves more explorations. In this study, we demonstrate that stearoyl-CoA desaturase 1 (SCD1) in subcutaneous WAT (scWAT) responded to cold stimulation and was able to promote mobilization of triacylglycerol [TAG (triglyceride)]. In vitro studies showed that SCD1 promoted lipolysis in C3H10T1/2 white adipocytes. The lipolytic effect was contributed by one of SCD1's products, oleic acid (OA). OA upregulated adipose TAG lipase and hormone-sensitive lipase expression. When SCD1 was overexpressed in the scWAT of mice, lipolysis was enhanced, and oxygen consumption and heat generation were increased. These effects were also demonstrated by the SCD1 knockdown experiments in mice. In conclusion, our study suggests that SCD1, known as an enzyme for lipid synthesis, plays a role in upregulating lipid mobilization through its desaturation product, OA.
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http://dx.doi.org/10.1194/jlr.RA120000869DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7707166PMC
December 2020

Associations between dietary patterns and physical fitness among Chinese elderly.

Public Health Nutr 2020 Sep 18:1-8. Epub 2020 Sep 18.

Department of Toxicology and Nutrition, School of Public Health, Cheeloo College of Medicine, Shandong University, 44, Wenhuaxi Street, Jinan250012, People's Republic of China.

Objective: To explore the relationship between dietary patterns and physical fitness among older Chinese (≥60 years) individuals.

Design: Cross-sectional survey. Dietary data were collected by a simplified semi-quantitative FFQ. The 30-s Chair Stand test (30sCST), 30-s Arm Curl test (30sACT), 8-foot Time Up-and-Go test (8fTUAGT) and 6-min Walking test (6mWT) were used to assess physical fitness. Dietary patterns were obtained by factor analysis. The association between dietary patterns and physical fitness was explored by multiple logistic regression.

Setting: Six communities (villages) of three districts in Liaocheng City (Shandong Province, China).

Participants: A total of 596 residents were recruited from April to May 2017.

Results: Among 556 residents who were finally enrolled, 196 were men (35 %) and 360 were women (65 %). Three dietary patterns were identified: 'Western', 'Vegetarian' and 'Modern'. The 30sACT revealed that men in the fourth quartile of the Western pattern were less likely to be classified in the 'high-level' group, but men in the fourth quartile of the Vegetarian pattern were classified in the high-level group. The 6mWT revealed that men in the fourth quartile of the Modern pattern were classified in the high-level group. These associations were independent of confounding factors.

Conclusions: Adherence to the Vegetarian pattern and Modern pattern may be protective factors for maintaining good physical fitness in older Chinese individuals. The Western pattern may lead to poor physical fitness in this population.
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http://dx.doi.org/10.1017/S136898002000333XDOI Listing
September 2020

Neutrophil extracellular trap from Kawasaki disease alter the biologic responses of PBMC.

Biosci Rep 2020 09;40(9)

Department of Vascular Surgery, Affiliated Hospital of Chengde Medical University, No. 36, Nanyingzi Street, Shuangqiao District, Chengde, Hebei 067000, China.

Kawasaki disease (KD), also known as mucocutaneous lymph node syndrome, is an acute systemic vasculitis syndrome that mainly occurs in infants under 5 years of age. In the current manuscript, we were aiming to analyze the role of neutrophil extracellular traps (NETs) in the pathogenesis of KD, especially their interplay with peripheral blood mononuclear cells (PBMCs). Neutrophils were exposed to 20 nM phorbol myristate acetate (PMA), we found that neutrophils of KD patients were more likely to form NETs compared with healthy controls (HCs). Furthermore, PBMCs were cultured with NETs for 24 h, and we observed that NETs significantly increased the cell viability, suppressed cell apoptosis, and enhanced the pro-inflammatory cytokines production and NF-κB activation in PBMCs from KD patients. In addition, with the stimulation of NETs, the expression of vascular endothelial growth factor A (VEGF-A) and hypoxia-inducible factor-1α (HIF-1α) were increased, which were related with the pathological mechanism of KD. At last, we examined the activation of phosphoinositide 3 kinase (PI3K)/Akt signaling, and we found NETs treatment obviously enhanced the activation of PI3K and Akt. In conclusion, these findings suggested that the formation of NETs may alter the biologic responses of PBMC and affect the vascular injury in KD.
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http://dx.doi.org/10.1042/BSR20200928DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7477316PMC
September 2020

Enabling Superior Sodium Capture for Efficient Water Desalination by a Tubular Polyaniline Decorated with Prussian Blue Nanocrystals.

Adv Mater 2020 Aug 12;32(33):e1907404. Epub 2020 Jul 12.

College of Materials Science and Engineering, and State Key Laboratory Breeding Base of Green Chemistry Synthesis Technology, Zhejiang University of Technology, 18 Chaowang Road, Hangzhou, 310014, China.

The application of electrochemical energy storage materials to capacitive deionization (CDI), a low-cost and energy-efficient technology for brackish water desalination, has recently been proven effective in solving problems of traditional CDI electrodes, i.e., low desalination capacity and incompatibility in high salinity water. However, Faradaic electrode materials suffer from slow salt removal rate and short lifetime, which restrict their practical usage. Herein, a simple strategy is demonstrated for a novel tubular-structured electrode, i.e., polyaniline (PANI)-tube-decorated with Prussian blue (PB) nanocrystals (PB/PANI composite). This composite successfully combines characteristics of two traditional Faradaic materials, and achieves high performance for CDI. Benefiting from unique structure and rationally designed composition, the obtained PB/PANI exhibits superior performance with a large desalination capacity (133.3 mg g at 100 mA g ), and ultrahigh salt-removal rate (0.49 mg g s at 2 A g ). The synergistic effect, interfacial enhancement, and desalination mechanism of PB/PANI are also revealed through in situ characterization and theoretical calculations. Particularly, a concept for recovery of the energy applied to CDI process is demonstrated. This work provides a facile strategy for design of PB-based composites, which motivates the development of advanced materials toward high-performance CDI applications.
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http://dx.doi.org/10.1002/adma.201907404DOI Listing
August 2020

Phenylenediamine-Based Carbon Nanodots Alleviate Acute Kidney Injury via Preferential Renal Accumulation and Antioxidant Capacity.

ACS Appl Mater Interfaces 2020 Jul 2;12(28):31745-31756. Epub 2020 Jul 2.

Department of Urology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Institute of Urology Nanjing University, No. 321 Zhongshan Road, Nanjing 210008, Jiangsu, China.

As a reactive oxygen species (ROS)-promoted disease, acute kidney injury (AKI) is associated with high mortality and morbidity, but no effective pharmacological treatment is available. Kidney-targeted and ROS-reactive antioxidants are in urgent demand for AKI treatment. A promising nanotechnology-based strategy for targeting renal tubules offers new perspectives for AKI treatment but remains challenging because of the glomerular filtration barrier, which requires ultrasmall-sized therapeutics for penetration and filtration. Here, we fabricated four potential antioxidative carbon nanodots (CNDs) with ultrasmall size. After balancing the antioxidant properties and biocompatibility, -phenylenediamine-based CNDs (PDA-CNDs) were chosen for further research. PDA-CNDs demonstrated remarkable antioxidant properties for scavenging multiple toxic free radicals, enabling efficient protection of cells under various oxidative stresses in vitro. Moreover, fluorescence imaging revealed that PDA-CNDs preferentially accumulated in the injured kidney of mice with ischemia-reperfusion (IR)-induced AKI. Blood renal function tests and kidney tissue staining revealed the therapeutic efficacy of PDA-CNDs for AKI in both the murine IR-induced AKI model and cisplatin-induced AKI model. Collectively, this is the first study revealing that specific rationally designed CNDs could be a promising pharmacological treatment for AKI induced by ROS.
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http://dx.doi.org/10.1021/acsami.0c05041DOI Listing
July 2020

A Longitudinal Study of a Multicomponent Exercise Intervention with Remote Guidance among Breast Cancer Patients.

Int J Environ Res Public Health 2020 05 14;17(10). Epub 2020 May 14.

Department of Science and Technology, Shandong Institute of Commerce and Technology, Jinan 250103, China.

: Breast cancer patients in treatment suffer from long-term side effects that seriously influence their physical and mental health. The aim of this study was to examine effectiveness of a 12-week multicomponent exercise (ME) with remote guidance intervention on health-related outcomes after one year among breast cancer patients. : In phases I-III, 60 patients (51.2 ± 7.9 years) with breast cancer (BC) who completed chemotherapy/postoperative radiotherapy within the previous four months to two years were randomly assigned to 1) multicomponent exercise with remote guidance (ME) and 2) usual care (UC). Eligible participants were approached to assess cancer-related quality of life (QOL), muscle strength, cardiorespiratory endurance, and physical activity (PA) barriers after one year. : The results demonstrated that, after one year, the ME group reported higher vitality-related QOL (5.776, 95% confidence interval [CI] 0.987, 10.565, effect size [ES] = 0.360), mental health-related QOL (9.938, 95% CI 4.146, 15.729, ES = 0.512), leg strength and endurance (2.880, CI 1.337, 4.423, ES = 0.557), and strength and endurance of upper extremities (2.745, 95% CI 1.076, 4.415, ES = 0.491) and lower physical activity (PA) hindrance (5.120, 95% CI 1.976, 8.264, ES = 0.486) than the UC group. : The ME group observed significant differences from the UC group in QOL, muscle strength, cardiopulmonary endurance, and PA participation. These findings suggested that the multicomponent exercise intervention with remote guidance produced long-term health benefits for breast cancer patients.
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http://dx.doi.org/10.3390/ijerph17103425DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7277866PMC
May 2020

Ethacrynic acid targets GSTM1 to ameliorate obesity by promoting browning of white adipocytes.

Protein Cell 2021 Jun;12(6):493-501

Key Laboratory of Metabolism and Molecular Medicine, The Ministry of Education, Department of Biochemistry and Molecular Biology, Shanghai Medical College, Fudan University, Shanghai, 200030, China.

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http://dx.doi.org/10.1007/s13238-020-00717-7DOI Listing
June 2021

Clinical significance of progranulin correlated with serum soluble Oxford 40 ligand in primary Sjögren's syndrome.

Medicine (Baltimore) 2020 May;99(18):e19967

Department of Rheumatism and Immunology, The Second Hospital of Hebei Medical University, Shijiazhuang, P.R. China.

The present study aimed to investigate the association between the expressions of serum progranulin (PGRN) and serum soluble Oxford 40 ligand (sOX40L) and determine their clinical significances in primary Sjögren's syndrome (pSS).The present study included a total of 68 patients with pSS and 50 healthy controls. Demographic data and clinical basic information were collected. Enzyme-linked immunosorbent assay (ELISA) was performed to determine serum levels of PGRN, sOX40L and interleukins. Spearman's correlation coefficient and Mann-Whitney U test were used to determine the correlation between PGRN, and sOX40L and the association between PGRN and sOX40L and disease activity and disease severity.Serum interleukin (IL)-4, IL-6, IL-10, PGRN, and sOX40L levels were significantly higher in pSS patients as compared to the healthy controls. A positive correlation was observed between PGRN and sOX40L. Patients with elevated levels of PGRN or sOX40L exhibited higher disease activity compared to those with lower levels. Patients with III to IV stages of pSS or multiple system damage showed higher serum levels of PGRN and sOX40L.Elevated serum PGRN, and sOX40L levels were relevant with disease activity and severity in patients with pSS.
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http://dx.doi.org/10.1097/MD.0000000000019967DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7440338PMC
May 2020

Surface/Interface Engineering for Constructing Advanced Nanostructured Photodetectors with Improved Performance: A Brief Review.

Nanomaterials (Basel) 2020 Feb 19;10(2). Epub 2020 Feb 19.

Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China.

Semiconductor-based photodetectors (PDs) convert light signals into electrical signals via a photon-matter interaction process, which involves surface/interface carrier generation, separation, and transportation of the photo-induced charge media in the active media, as well as the extraction of these charge carriers to external circuits of the constructed nanostructured photodetector devices. Because of the specific electronic and optoelectronic properties in the low-dimensional devices built with nanomaterial, surface/interface engineering is broadly studied with widespread research on constructing advanced devices with excellent performance. However, there still exist some challenges for the researchers to explore corresponding mechanisms in depth, and the detection sensitivity, response speed, spectral selectivity, signal-to-noise ratio, and stability are much more important factors to judge the performance of PDs. Hence, researchers have proposed several strategies, including modification of light absorption, design of novel PD heterostructures, construction of specific geometries, and adoption of specific electrode configurations to modulate the charge-carrier behaviors and improve the photoelectric performance of related PDs. Here, in this brief review, we would like to introduce and summarize the latest research on enhancing the photoelectric performance of PDs based on the designed structures by considering their surface/interface engineering and how to obtain advanced nanostructured photo-detectors with improved performance, which could be applied to design and fabricate novel low-dimensional PDs with ideal properties in the near future.
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http://dx.doi.org/10.3390/nano10020362DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7075325PMC
February 2020

Enhanced sodium storage kinetics by volume regulation and surface engineering via rationally designed hierarchical porous [email protected]/rGO.

Nanoscale 2020 Feb;12(7):4341-4351

Pillar of Engineering Product Development, Singapore University of Technology and Design, 8 Somapah Road, 487372, Singapore.

Transition metal phosphides, such as iron phosphide (FeP), have been considered as promising anode candidates for high-performance sodium ion batteries (SIBs) owing to their high theoretical capacity. However, the development of FeP is limited by large volume change, low electrical conductivity and sluggish kinetics with sodium ions. Moreover, the sodium storage kinetics and dynamics behavior in FeP are still unclear. Herein, improved sodium storage ability of FeP is achieved by volume regulation and surface engineering via a rationally designed hierarchical porous [email protected]/rGO nanocomposite. This [email protected]/rGO nanocomposite exhibits excellent rate capability and long cycle life as the anode of SIBs. Specifically, the [email protected]/rGO nanocomposite delivers high specific capacities of 635.7 and 343.1 mA h g-1 at 20 and 2000 mA g-1, respectively, and stable cycling with 88.2% capacity retention after 1000 cycles. The kinetics and dynamics studies demonstrate that the superior performance is attributed to the rationally designed hierarchical porous [email protected]/rGO with a high capacitive contribution of 93.9% (at 2 mV s-1) and a small volume expansion of only 54.9% by in situ transmission electron microscopy (TEM) measurement. This work provides valuable insights into understanding the phase evolution of FeP during the sodiation/desodiation process for designing high-performance SIBs.
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http://dx.doi.org/10.1039/c9nr09278aDOI Listing
February 2020

Glide Mirror Plane Protected Nodal-Loop in an Anisotropic Half-Metallic MnNF Monolayer.

J Phys Chem Lett 2020 Jan 6;11(2):485-491. Epub 2020 Jan 6.

Spintronics Institute, School of Physics and Technology , University of Jinan , Jinan 250022 , China.

Two-dimensional (2D) nodal-loop (NL) semimetals have attracted tremendous attention for their abundant physics and potential device applications, whereas the realization of gapless NL semimetals robust against spin-orbit coupling (SOC) remains a big challenge. Recently, breakthroughs have been made with the realization of gapless NL semimetals in 2D half-metallic materials, where NLs were protected by a horizontal mirror plane symmetry. Here we first propose an alternative nonsymmorphic horizontal glide mirror plane symmetry which could protect the NLs in 2D materials. On the basis of comprehensive first-principles calculations and symmetry analysis, we found that the glide mirror symmetry together with intrinsic out-of-plane spin polarization can protect the NL against SOC in a half-metallic semimetal, namely, the MnNF monolayer. Moreover, we predict that the MnNF monolayer has strong anisotropic characteristics, tunable band structure by changing the magnetization direction, and 100% spin-polarized transport properties. Our work not only provides a novel 2D half-metallic semimetal with strong anisotropy but also broadens the scope of 2D nodal-loop materials.
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http://dx.doi.org/10.1021/acs.jpclett.9b03320DOI Listing
January 2020

A digital guiding device to facilitate cementation of porcelain laminate veneers.

J Prosthet Dent 2020 Oct 13;124(4):411-415. Epub 2019 Dec 13.

Associate Professor, Department of Prosthodontics, Stomatological Digital Engineering Center, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, PR China. Electronic address:

This article presents a computer-aided design and computer-aided manufacturing (CAD-CAM) guiding device to facilitate the simultaneous preconditioning and cementation of multiunit porcelain laminate veneers (PLVs). The guiding device was designed from the digital cast of the PLVs and definitive cast assembly, with gingival margins 2 mm from the PLV margins and lingual perforations and milled from a transparent polymethyl methacrylate (PMMA) disk. The PLVs were seated in the guiding device during the preconditioning procedures and transferred to the abutment teeth with luting cement loaded on the intaglio surfaces. Excess resin cement was removed while the guiding device held the PLVs in place. This technique provides predictable, accurate, and efficient simultaneous preconditioning and cementing of PLVs.
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http://dx.doi.org/10.1016/j.prosdent.2019.10.011DOI Listing
October 2020

High Precision Dimensional Measurement with Convolutional Neural Network and Bi-Directional Long Short-Term Memory (LSTM).

Sensors (Basel) 2019 Dec 2;19(23). Epub 2019 Dec 2.

School of Automation and Electrical Engineering, University of Science and Technology Beijing, Beijing 100083, China.

In modern industries, high precision dimensional measurement plays a pivotal role in product inspection and sub-pixel edge detection is the core algorithm. Traditional interpolation and moment methods have achieved some success. However, those methods still have shortcomings. For example, the accuracy is still insufficient with the resolution limitation of the image sensor. Moreover, prediction results can be affected by image noise. With the recent success of deep learning technology, we propose a sub-pixel edge detection method based on convolution neural network (CNN) and bi-directional long short-term memory (LSTM). First, one-dimensional visual geometry group-16 (VGG-16) is employed to extract edge features. Then, a transformation operation is developed to generate sequence information. Lastly, bi-directional LSTM with fully-connected layers is introduced to output edge positions. Experimental results on our steel plate dataset demonstrate that our method achieves superior accuracy and anti-noise ability than traditional methods.
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http://dx.doi.org/10.3390/s19235302DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6928596PMC
December 2019

A Chitosanase mutant from Streptomyces sp. N174 prefers to produce functional chitopentasaccharide.

Int J Biol Macromol 2020 May 15;151:1091-1098. Epub 2019 Nov 15.

Endoscopy Center, China Japan Union Hospital of Jilin University, Changchun 130033, China. Electronic address:

The Chitosanase (Genbank No. AAA19865.1, SsCsn46) from Streptomyces sp. N174 was mutated via the deletion of 198A, 199A, 200H and 201D. Mutant chitosanase (m-SsCsn46) and wild chitosanase (SsCsn46) were secretory expressed at high level and purity in Pichia pastoris GS115. The activity of m-SsCsn46 was 30,000 U/mg and reduced by almost 40% of the maximum activity of wild type SsCsn46 (50,000 U/mg). Both enzymes had a high maximum activity at pH 6.0 and 50 °C, and maintained 40% of maximum activity at temperature 40-60 °C at pH 6.0 and or pH 5-7 at 50 °C after 2-hour treatment. The hydrolysis of chitosan by SsCsn46 yielded predominantly chitooligosaccharides with degree of polymerization (DP) 3-5 while m-SsCsn46 preferred to produce chitopentasaccharide from chitosan. Chitopentasaccharide improved gut microbiota significantly (by reducing the proportion of harmful pathogen population and increasing the proportion of the probiotic population) in mice when compared with the chitooligosaccharides with DPs 3-5. Thus, the mutant chitosanase from Streptomyces sp. N174 prefers to produce functional chitopentasaccharide from chitosan and m-SsCsn46 provides a potential tool for producing high-purity functional chitooligosaccharides.
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http://dx.doi.org/10.1016/j.ijbiomac.2019.10.151DOI Listing
May 2020