Publications by authors named "Melanie Moreau"

3 Publications

  • Page 1 of 1

Principles and analytical performance of Papilloplex® HR-HPV, a new commercial CE-IVD molecular diagnostic test for the detection of high-risk HPV genotypes.

Diagn Microbiol Infect Dis 2019 Sep 25;95(1):46-54. Epub 2019 Apr 25.

GeneFirst Ltd, Culham Science Centre, Abingdon, Oxfordshire, OX14 3DB, UK. Electronic address:

The accurate detection and genotyping of high-risk human papillomavirus (HR-HPV) are critical for cervical cancer screening and epidemiological investigations. GeneFirst Papilloplex® HR-HPV is a new CE-IVD-marked real-time PCR test based on patented multiplex probe amplification technology. Papilloplex® HR-HPV provides the simultaneous detection and differentiation of 14 HR-HPV genotypes (16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and 68a/b) in a single closed-tube reaction ensuring rapid, cost-effective, and contamination-free results. In this study, the analytical performance characteristics in terms of the assay's sensitivity, specificity, range, reproducibility, and cross-reactivity were evaluated. Papilloplex® HR-HPV provided sensitive detection and differentiation of 14 HR-HPV types with highly reproducible results. The differential HR-HPV specificity and sensitivity were further confirmed through the participation in the WHO HPV Laboratory Network Proficiency Study (2014). Overall, GeneFirst Papilloplex® HR-HPV assay demonstrated a robust analytical performance with reproducible and reliable results in the detection of HR-HPV genotypes.
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http://dx.doi.org/10.1016/j.diagmicrobio.2019.04.005DOI Listing
September 2019

Counting the number of proteins coupled to single nanoparticles.

J Am Chem Soc 2007 Oct 29;129(42):12592-3. Epub 2007 Sep 29.

Laboratoire d'Optique et Biosciences and Laboratoire de Physique de la Matière Condensée, Ecole Polytechnique, CNRS, 91128 Palaiseau, France, and INSERM U696, 91128, Palaiseau, France.

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http://dx.doi.org/10.1021/ja0731975DOI Listing
October 2007

Modification of the surface properties of porous nanometric zirconia particles by covalent grafting.

Langmuir 2004 Apr;20(8):3449-55

Laboratoire de Physique de la Matière Condensée, CNRS UMR 7643C, Ecole Polytechnique, 91128 Palaiseau Cedex, France.

We here report on the covalent grafting of various phosphated species (phosphoric acid, phenylphosphonic acid, and octyl phosphate) onto the surface of monoclinic zirconia nanoparticles obtained by hydrothermal treatment of zirconium acetate. The initial particles are 60 nm aggregates of nanometric primary grains and present an inner porosity. Small-angle X-ray scattering shows that the high specific area of the colloidal particles (450 m2 x g(-1)) decreases to 150 m2 x g(-1) upon drying. Therefore, phosphated reactants can access the whole internal surface of the aggregates only before drying. The surface of the particles can be covered with functional groups bound through a variable number of Zr-O-P bonds. Several factors probably enhance the reaction between the particles and the phosphates or phosphonates: the large specific area of the particles, a fully accessible porous network, and a large concentration of surface terminal groups. At the same time, the morphology of the particles is well preserved upon grafting. This is due to the good crystallinity of the primary grains that constitute the particles. In addition, the grafting drastically modifies the surface properties of the colloids. For example, the polarizability of the particles decreases in the sequence -POH > as-prepared ZrO2 > -PC6H5 > -POC8H17. Furthermore, the grafting of octyl phosphate allows exclusion of water from pores of 2 nm radius, up to hydrostatic pressures of 20 MPa.
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http://dx.doi.org/10.1021/la036249mDOI Listing
April 2004