Publications by authors named "Melania Mikołajczyk"

3 Publications

  • Page 1 of 1

Tumour protein 53 is linked with type 2 diabetes mellitus.

Indian J Med Res 2017 Aug;146(2):237-243

Department of Internal Disease, Diabetology & Clinical Pharmacology, Lodz, Poland.

Background & Objectives: Tumour protein p53 (TP53) is a stress sensitive transcription factor responsible for the control of cell survival and death to prevent from tumour formation. In vitro and animal studies have indicated that TP53 also responds to metabolic changes and influences metabolic pathways. This study was undertaken to determine the serum level of TP53 and its correlations with clinical and biochemical parameters in type 2 diabetes mellitus (T2DM) patients in comparison to non-diabetic control individuals.

Methods: An observational study was conducted between December 2009 and November 2013 to evaluate TP53 serum level using ELISA. Cases (n=225) were defined as patients who were diagnosed with T2DM. Non-diabetic controls (n=255) were matched by age and sex. Multivariable modelling using logistic regression examined associations between clinical characteristics and TP53 level or T2DM predication was performed.

Results: Serum TP53 level was significantly higher in T2DM patients as compared to non-diabetic healthy controls (1.69 vs 2.07 ng/ml, P<0.001). In T2DM patients, the level of TP53 increased with the age, duration of diabetes and waist-to-hip ratio (WHR) value. A logistic regression analysis revealed that increased serum TP53 level was significantly associated with family history of diabetes, age and WHR. Moreover, TP53, triglyceride and body mass index could be used to predict T2DM.

Interpretation & Conclusions: Our results suggest that TP53 may be linked with T2DM. The fluctuations of serum TP53 level may reflect metabolic and oxidative stress associated with chronic hyperglycaemia. Further studies need to be done to confirm these findings.
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http://dx.doi.org/10.4103/ijmr.IJMR_1401_15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5761034PMC
August 2017

Association between single nucleotide polymorphisms of the G-protein γ5 subunit and the risk of essential hypertension in the population of Poland.

Pol Arch Med Wewn 2015 16;125(12):903-9. Epub 2015 Nov 16.

Introduction: Polymorphisms in genes coding G-protein subunits (α, β, and γ) may affect the response of stimulated α2A-adrenergic receptors, which are involved in the regulation of blood pressure. OBJECTIVES The aim of the present study was to determine the association between the rs11559300 (A/G), rs199705300 (C/A), rs61754630 (C/T), rs13093 (C/A), and rs41284589 (C/T) single nucleotide polymorphisms (SNPs) of the gene coding G-protein γ5 subunit (GNG5) and the risk of essential hypertension in the population of Poland.

Patients And Methods: A total number of 838 subjects were included in the study: 536 patients with diagnosed essential hypertension and 302 controls. Genotyping was performed using the polymerase chain reaction-restriction length polymorphism (PCR-RFLP) method.

Results: Of the studied GNG5 polymorphisms, only SNP rs13093 was significantly associated with an increased risk of essential hypertension (odds ratio [OR], 2.91; 95% confidence interval [CI], 1.68-5.05; P = 0.0036). In addition, the T allele of rs41284589 may protect against hypertriglyceridemia (OR, 0.32; 95% CI, 0.1-0.9).

Conclusions: rs13093 in the promoter region of GNG5 may be associated with an increased risk of essential hypertension in the Polish population. Further studies are needed to explain the molecular mechanism by which rs13093 affects blood pressure.
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http://dx.doi.org/10.20452/pamw.3207DOI Listing
March 2017

[The alternative method for albuminuria determination: second morning urine sample instead of 24-hour urine collection].

Pol Merkur Lekarski 2013 May;34(203):255-8

Zakład Diagnostyki Laboratoryjnej i Biochemii Klinicznej, Uniwersytet Medyczny w Łodzi.

Unlabelled: Urinary albumin excretion is an established risk factor for renal and cardiovascular events. Measurement of albumin in the urine daily collection is the gold standard in assessing albuminuria. The 24-hour urine collection is cumbersome procedure that generates a lot of mistakes, therefore other methods have been proposed. The aim of the study was to compare the assessment of albuminuria in the 24-hour urine collection and in the second morning urine sample as well as to determine UACR.

Material And Methods: The study included 32 patients, from whom the daily and the second morning urine samples were collected. In both samples, the albumin and creatinine concentrations were determined and the urinary albumin: creatinine ratio (UACR) was calculated.

Results: An excellent correlation between the UACR determined from the 24-hour urine collection and the other portion of the second morning sample was obtained within a wide range of albuminuria values (r = 0.9825). Furthermore, a better correlation between the same characteristics was obtained in urine of patients with normoalbuminuria when UACR did not exceed 30 mg/g creatinine (r = 0.9771). Above this value, the correlation was slightly lower for micro- and macroalbuminuria and equalled 0.9249 and 0.9332, respectively.

Conclusions: On the basis of the obtained results it can be concluded that the second morning urine sample with the determination of UACR is a good alternative to the 24-hour urine collection and the first morning urine sample which are burdened with a preanalytical error.
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May 2013