Publications by authors named "Meirina Gartika"

2 Publications

  • Page 1 of 1

Effectiveness of Bioactive Compound as Antibacterial and Anti-Quorum Sensing Agent from : An In Silico Study.

Molecules 2021 Apr 23;26(9). Epub 2021 Apr 23.

Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Padjadjaran, Sumedang 45363, Indonesia.

Background: antibiotic resistance encourages the development of new therapies, or the discovery of novel antibacterial agents. Previous research revealed that (Sarang Semut) contain potential antibacterial agents. However, specific proteins inhibited by them have not yet been identified as either proteins targeted by antibiotics or proteins that have a role in the quorum-sensing system. This study aims to investigate and predict the action mode of antibacterial compounds with specific proteins by following the molecular docking approach.

Methods: butein (), biflavonoid (), 3″-methoxyepicatechin-3--epicatechin (), 2-dodecyl-4-hydroxylbenzaldehyde (), 2-dodecyl-4-hydroxylbenzaldehyde (), pomolic acid (), betulin (), and sitosterol-(6'--tridecanoil)-3---D-glucopyranoside () from act as the ligand. Antibiotics or substrates in each protein were used as a positive control. To screen the bioactivity of compounds, ligands were analyzed by Prediction of Activity Spectra for Substances (PASS) program. They were docked with 12 proteins by AutoDock Vina in the PyRx 0.8 software application. Those proteins are penicillin-binding protein (PBP), MurB, Sortase A (SrtA), deoxyribonucleic acid (DNA) gyrase, ribonucleic acid (RNA) polymerase, ribosomal protein, Cytolysin M (ClyM), FsrB, gelatinase binding-activating pheromone (GBAP), and PgrX retrieved from UniProt. The docking results were analyzed by the ProteinsPlus and Discovery Studio software applications.

Results: most compounds have Pa value over 0.5 against proteins in the cell wall. In nearly all proteins, biflavonoid () has the strongest binding affinity. However, compound binds only three residues, so that is the non-competitive inhibitor.

Conclusion: compound can be a lead compound for an antibacterial agent in each pathway.
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http://dx.doi.org/10.3390/molecules26092465DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8122932PMC
April 2021

A terpenoid isolated from sarang semut (Myrmecodia pendans) bulb and its potential for the inhibition and eradication of Streptococcus mutans biofilm.

BMC Complement Altern Med 2018 May 9;18(1):151. Epub 2018 May 9.

Department of Oral Biology, Faculty of Dentistry, Universitas Padjadjaran, Bandung, Indonesia.

Background: Dental caries remains a serious problem due to its detrimental effects on individual health and quality of life. The bulbs of Myrmecodia pendans (Merr & Perry), native plants of Papua, have been used as natural remedies for tumours, gout, diarrhoea, and fever. In this study, one of the active compounds of M. pendans was isolated, and its biological activity against the formation of Streptococcus mutans ATCC 25175 biofilm was tested.

Methods: M. pendans was extracted with ethyl acetate using a Soxhlet apparatus. The extract was then separated, and chromatographic purification provided the isolated compound. The structure of the active compound was then characterized using UV, IR, NMR, and MS spectrometry. The obtained compound was added to S. mutans biofilms to determine the MBIC and MBEC values.

Results: The compound isolated from M. pendans was determined to be a labdane diterpene derivative with the formula CHO. The MBIC value of the terpenoid towards the S. mutans biofilms was 50 ppm, and the MBEC value for the 1 min induction time was 40%.

Conclusion: The terpenoid extracted from M. pendans has the potential to be developed into an antibacterial agent particularly for preventing the formation of biofilms.
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http://dx.doi.org/10.1186/s12906-018-2213-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5941495PMC
May 2018
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