Publications by authors named "Meili Xu"

17 Publications

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Physiological responses and health risks of edible amaranth under simultaneous stresses of lead from soils and atmosphere.

Ecotoxicol Environ Saf 2021 Oct 28;223:112543. Epub 2021 Jul 28.

Guangdong Research Center for Industrial Contaminated Site Remediation Technology and Equipment Engineering, School of Environmental Science and Engineering, Guangdong University of Technology, Guangzhou 510006, PR China.

Lead (Pb) is widely distributed in the environment that can impose potential risks to vegetables and humans. In this work, we conducted a pot experiment in Southern China to examine the physiological response and risk of edible amaranth (Amaranthus tricolor L.) under the simultaneous stresses of lead from soil and atmosphere. The results indicate that the lead content of amaranth substantially exceeded China's national standard when Pb concentration from soils and atmosphere was high, and comparing to teenagers and adults, children exposed a higher health risk after consuming the contaminated amaranth. Under the co-stress, the lead in roots of amaranth mainly came from the soil, but the Pb from atmospheric deposition can significantly affect the lead concentration in leaves. While lead from atmospheric deposition is found to promote the growth of amaranth, the stress of lead from the soils shows an inhibitory effect, as indicated by the increase in HO content, the damage in cell membranes, and the limitation in chlorophyll synthesis. The antioxidant system in stems and leaves of amaranth can effectively alleviate the Pb toxicity. However, the stress of high lead concentration from soils can substantially suppress the antioxidant enzyme activity of roots. While it is found that heavy metals in soils can significantly affect the vegetables grown in a multi-source pollution environment, we also call for the attention on the potential health risk imposed by the lead from atmospheric deposition. This study provides an important reference for the prevention and control of crop contamination in multi-source pollution environments.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112543DOI Listing
October 2021

HIF‑1α protein SUMOylation is an important protective mechanism of action of hypothermia in hypoxic cardiomyocytes.

Mol Med Rep 2021 06 26;23(6). Epub 2021 Apr 26.

Central Laboratory, The Fifth Central Hospital of Tianjin, Tianjin 300450, P.R. China.

Different degrees of myocardial ischemia‑reperfusion injury during open‑heart surgery are inevitable. Therapeutic hypothermia is an important technique for reducing ischemia‑reperfusion injury; however, there are numerous potential adverse effects. Furthermore, the underlying molecular mechanisms of action of therapeutic hypothermia remain unclear. In the present study, rat hearts were perfused for 30 min and subjected to 30 min of regional ischemia, followed by 120 min of reperfusion. Animals received intraperitoneal injection of spectomycin B1 at 30 min prior to the start of surgery. Total myocardial area, infarct area, myocardial injury, and apoptosis were assessed. H9C2 cells were incubated for 24  h at 34˚C with 5% CO to simulate therapeutic hypothermic stress, and cell viability and mitochondrial injury were evaluated. The levels of protein SUMOylation, hypoxia‑inducible factor (HIF)‑1α and vascular endothelial growth factor (VEGF) were determined by western blot analysis. It was demonstrated that hypoxia significantly increased the overall modification by the small ubiquitin‑related modifier protein (SUMO) of various proteins in cardiomyocytes, both and . In turn, this increased the protein levels of HIF‑1α, continuously stimulated downstream VEGF expression. Therapeutic hypothermia further increased protein SUMOylation, whereas inhibiting the SUMOylation pathway reduced the protective effect of therapeutic hypothermia on hypoxic cardiomyocytes. Overall, these data suggested that increasing SUMOylation of HIF‑1α may be an important molecular mechanism underlying the protective effects of therapeutic hypothermia following hypoxia in myocardial cells. These findings may aid in the use of therapeutic hypothermia for treatment of myocardial ischemia‑reperfusion and help avoid excessive side effects.
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http://dx.doi.org/10.3892/mmr.2021.12115DOI Listing
June 2021

Solution-Processed Efficient Perovskite Nanocrystal Light-Emitting Device Utilizing Doped Hole Transport Layer.

J Phys Chem Lett 2021 Jan 11;12(1):94-100. Epub 2020 Dec 11.

State Key Laboratory of Integrated Optoelectronics and College of Electronic Science and Engineering, Jilin University, Changchun 130012, China.

Light-emitting devices (LEDs) with inorganic perovskite nanocrystals (PNCs) fabricated through the all-solution process have tremendous potential for new-generation illumination and displays on account of their large area and cost-effective manufacturing. However, the development of efficient solution-processed PNC LEDs remains challenge, which mainly results from the fact that only a few types of charge transport layers can be employed for the subsequent deposition steps, thus leading to injection barriers and charge injection imbalance inside these LEDs. Herein 4,4'-bis(carbazole-9-yl) biphenyl (CBP) is introduced as a dopant into the poly(9,9-dioctylfluorene---(4-(3-methylpropyl)) diphenylamine) (TFB) hole transport layer (HTL), which efficiently modulates the mobility of charge carrier as well as the energy level of the HTL, resulting in the barrier-free injection of the charge carrier in the as-fabricated solution-processed PNC LEDs. Consequently, the luminance of red LEDs (688 nm) reaches 2990 cd m, and the external quantum efficiency achieves 8.1%, which is the optimal performance for solution-processed PNC LEDs to date. Additionally, the turn-on voltage and roll-off have also been improved by the more balanced charge injection.
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http://dx.doi.org/10.1021/acs.jpclett.0c03047DOI Listing
January 2021

Anti-hyperuricemic potential of stevia (Stevia rebaudiana Bertoni) residue extract in hyperuricemic mice.

Food Funct 2020 Jul;11(7):6387-6406

Beijing Advance Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives School of Food and Chemical Technology, Beijing Technology and Business University, Beijing 100048, China.

Hyperuricemia (HUA) is considered a potent risk factor for the development of gout, renal failure, and cardiovascular disease. The current project was designed to use stevia (Stevia rebaudiana Bertoni) byproduct, named stevia residue extract (STVRE), for the treatment of HUA. Male Kunming mice were divided into six groups: normal control, model control, positive control (allopurinol, 5 mg per kg body weight [bw]), STVRE-1 (75 mg per kg bw), STVRE-2 (150 mg per kg bw), and STVRE-3 (300 mg per kg bw). HUA was induced by the administration of potassium oxonate (100 mg per kg bw), fructose (10% w/v), and yeast extract (100 mg per kg bw) for 8 weeks. STVRE significantly (p < 0.05) decreased uric acid (UA) production and ameliorated UA excretion by interacting with urate transporters. The STVRE remarkably attenuated oxidative stress mediated by UA and downregulated inflammatory-related response markers such as COX-2, NF-κB, PGE2, IL-1β, and TNF-α. Furthermore, STVRE also reversed HUA-induced abnormalities in kidneys compared with the MC group. The results of our study suggest that STVRE has potential to attenuate hyperuricemia and renal protective effects, and may be used as a natural supplement for the possible treatment of UA-related disorders.
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http://dx.doi.org/10.1039/c9fo02246eDOI Listing
July 2020

Organic Transistor Nonvolatile Memory with Three-Level Information Storage and Optical Detection Functions.

ACS Appl Mater Interfaces 2020 May 30;12(19):21952-21960. Epub 2020 Apr 30.

College of Electronic Science and Engineering, Jilin University, 2699 Qianjin Street, Changchun 130012, China.

By the current processing technology, it is a challenge to obtain ultrahigh-density information storage in the conventional binary floating-gate-based organic field-effect transistor (FG-OFET) nonvolatile memories (NVMs). To develop a multilevel memory in one cell is a feasible solution. In this work, we demonstrate FG-OFET NVMs with an integrated polymer floating-gate/tunneling (I-FG/T) layer consisting of poly(9,9-dioctylfluorene--benzothiadiazole) (F8BT) and polystyrene. The photoelectric effect of organic/polymer semiconductors is used to improve the controllability of the polarity and the number of the charges stored in the floating-gate. The FG-OFET NVMs integrate light sensitivity and nonvolatile information storage functions. By selecting suitable optical and electrical programming/erasing conditions, three-level information storage states, corresponding to electron storage, approximate neutrality, and hole storage in the floating-gate, are achieved and freely switched to each other. The memory mechanism and the dependence of the memory performances on the F8BT contents in I-FG/T layers are investigated. As a result, good memory performances, with mobility larger than 1.0 cm V s, reliable three-level switching endurance over 100 cycles, and stable three-level retention capability over 20 000 s, are achieved in our memory. Furthermore, an imaging system with a nonvolatile information storage function is demonstrated in a 16 × 5 array of FG-OFET NVMs.
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http://dx.doi.org/10.1021/acsami.0c01162DOI Listing
May 2020

miR-203 Acts as an Inhibitor for Epithelial-Mesenchymal Transition Process in Diabetic Foot Ulcers via Targeting Interleukin-8.

Neuroimmunomodulation 2019 8;26(5):239-249. Epub 2019 Nov 8.

Department of Burn and Plastic Surgery, The Second Xiangya Hospital, Central South University, Changsha, China,

Objectives: As a complication of diabetes mellitus (DM), one of the leading causes for death and disability for DM patients is diabetic foot ulcers (DFUs). Epithelial to mesenchymal transition (EMT) plays a critical role in wound healing of DFUs. miR-203 is specifically enriched in keratinocytes and has been shown to target interleukin 8 (IL-8), which acts as an activator for the EMT process. In this study, we explored the interaction between miR-203 and IL-8 in DFU rat models and human keratinocyte cells, underlying the mechanism of miR-203's function in DFUs progression.

Methods: DFU rat models were used to test gene expression in DFU progression. Diabetic keratinocyte cell lines were used to validate in vitro. Wound healing and Transwell assays were applied to evaluate cell migration and invasion abilities. The EMT process was estimated by testing expression of E-cadherin, Vimentin and Slug. The interaction between miR-203 and IL-8 was determined by Luciferase assay.

Results: Our results demonstrated that the wound-healing process had been slowed in DFUs, and the advanced glycation end products (AGEs) and the receptor for advanced glycation end products (RAGEs) in wound tissue were of a higher expression than those in normal rat. miR-203 was increased in skin tissues from DFU rat models, while IL-8 was decreased. Through knock-down of miR-203 in AGE-treated keratinocyte cells, it had been shown that the downregulation of miR-203 could promote cell proliferation and migration, and facilitate the EMT process. Meanwhile, Luciferase assay proved that miR-203 could directly target and inhibit IL-8. The repression of IL-8 could rescue the outcomes brought about by miR-203 inhibition.

Conclusions: The upregulation of miR-203 in DFU tissues impaired wound healing by the repress EMT process. Specific knock-down of miR-203 could promote wound healing through the reactivation of its target gene IL-8 and the downstream IL-8/AKT pathway.
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http://dx.doi.org/10.1159/000503087DOI Listing
September 2020

MicroRNA-588 regulates invasion, migration and epithelial-mesenchymal transition via targeting EIF5A2 pathway in gastric cancer.

Cancer Manag Res 2018 30;10:5187-5197. Epub 2018 Oct 30.

Department of Gerontology, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, China,

Background: miRNAs are potential regulators of genes in many cancers. Here, we confirmed that the expression of miR-588 decreased in gastric cancer (GC) tissues and cells.

Materials And Methods: Sixty-seven GC tissues along with noncancerous tissues adjacent to them were included in the study. Quantitative real-time reverse transcription-PCR study was done to quantify the expression levels of mature miRNA. The expression of proteins was determined by Western blot and transwell chamber assay for invasion and migration studies. Immunohistochemical analysis and luciferase assay were done for evaluating the expression of epithelial-mesenchymal transition (EMT) markers and activity of EIF5A2, respectively. In vivo metastatic assay was done by injecting MGC-803 cells into nude mice.

Results: In the 5-year predicted survival study of GC patients included in the study, we found that miR-588 acted as a specific prognostic marker. Overexpression of miR-588 resulted in suppression of cell invasion, migration and progression of EMT, whereas suppression of miR-588 inverted the effects in both in vivo and in vitro experiments. miR-588 retained EIF5A2 by directly binding to the 3'-UTR. EIF5A2 was overexpressed in GC tissue samples, and the expression of miR-588 was inversely correlated to the levels of EIF5A2. The impact of miR-588 on invasion, migration and progression of EMT may be partially due to miR-588-mediated alterations of EiF5A2.

Conclusion: Overall, the findings of the study suggest that miR-588 acts as a tumor suppressor by regulating the invasion, migration and EMT via EIF5A2 pathway, hence presenting miR-588 as a prognostic marker as well as a therapeutic target for GC.
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http://dx.doi.org/10.2147/CMAR.S176954DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6215924PMC
October 2018

[Comparison of the efficacy and safety between endoscopic submucosal dissection and radical surgery for large colorectal laterally spreading tumors larger than 5 cm in diameter].

Zhong Nan Da Xue Xue Bao Yi Xue Ban 2018 Sep;43(9):1014-1019

Department of Gastroenterology, Second Xiangya Hospital, Central South University, Changsha 410011, China.

Objective: To compare the safety and efficacy between endoscopic submucosal dissection (ESD) and radical surgery (RS) for the treatment of large colorectal laterally spreading tumors (LST) larger than 50 mm in diameter. 
 Methods: From January 2011 to January 2016, a total of 82 patients were diagnosed as large LST without deep submucosal invasion (T1 SM2, ≥1 000 µm) in the Second Xiangya Hospital of Central South University. Among them, 52 patients were treated by ESD and the other 30 patients were treated by RS [laparoscopic-assisted colectomy (LAC)/open colectomy (OC)]. The clinic data were retrospectively analyzed and the en-bloc resection rate, en-bloc R0 resection rate, local recurrence, complication, procedure time and hospital stay were collected and analyzed.
 Results: The lesion sizes were (5.80±1.20) cm and (5.53±0.69) cm in diameter for ESD and RS groups, respectively (P>0.05). En-bloc resection rates, en-bloc R0 resection rates and recurrence rates showed no significant difference between the ESD group and RS group (P>0.05). Complication rate of the ESD group (7.69%, 4/52) was much lower than that in the RS group (33.33%, 10/30; P<0.01). The ESD group also had a shorter hospital stay and operation time than the RS group (P<0.05).
 Conclusion: ESD appears to be a safe, minimal invasive and effective strategy for treating large LST and it is obviously better than RS in the aspects of hospital stay, operation time and short-term complication.
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http://dx.doi.org/10.11817/j.issn.1672-7347.2018.09.013DOI Listing
September 2018

[Clinicopathological differences in laterally spreading tumors between rectum and colon].

Zhong Nan Da Xue Xue Bao Yi Xue Ban 2018 Feb;43(2):192-197

Department of Gastroenterology, Second Xiangya Hospital, Central South University, Changsha 410011, China.

Objective: To investigate the clinicopathological differences in laterally spreading tumor (LST) from the rectum and colon.
 Methods: Clinicopathological records of 198 patients with LST (116 cases in rectum, 82 cases in colon) from the Second Xiangya Hospital of Central South University between January 2012 and January 2017 were evaluated.
 Results: A total of 198 colorectal LST were included. According to the endoscopic classification, nodular mixed type (LST-GM), homogeneous type (LST-GH), flat elevated type(LST-FE) and pseudodepressed type (LST-PD) were 127(64.1%), 13(6.6%), 41(20.7%) and 17(8.6%), respectively. LST-GM was predominant in the rectum (71.7%), while LST-FE was predominant in the colon (78.0%), with significant difference (P<0.01). The mean size of LST was (52.03±35.62) mm or (25.37±11.56) mm in the rectum or the colon, with significant difference between them (P<0.01). High grade intraepithelial neoplasia frequency was higher in the rectum than that in the colon (31.0% vs 18.3%), while the low grade intraepithelial neoplasia frequency was lower in the rectum than that in the colon (61.2% vs 75.6%) (both P<0.05). The mean size of LST-GM and LST-GH diameter were larger in the rectum than that in the colon, and the malignant potential of LST-GM was higher in the rectum than that in the colon. The percentage of high grade intraepithelial neoplasia + invasive carcinoma was 41.8% and 22.2%, respectively (both P<0.05). LST in colon was mostly treated with endoscopic mucosal resection, while LST in rectum was treated by endoscopic submucosal dissection predominantly.
 Conclusion: LSTs from the rectum and colon show different clinicopathological characteristics to some extent. LST-GM is predominant in the rectum, while LST-FE is predominant in the colon. The malignant potential of LST-GM is higher in the rectum than that in the colon.
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http://dx.doi.org/10.11817/j.issn.1672-7347.2018.02.015DOI Listing
February 2018

Excellent low-voltage operating flexible ferroelectric organic transistor nonvolatile memory with a sandwiching ultrathin ferroelectric film.

Sci Rep 2017 08 21;7(1):8890. Epub 2017 Aug 21.

State Key Laboratory on Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University, 2699 Qianjin Street, Changchun, 130012, China.

The high operating voltage is a primary issue preventing the commercial application of the ferroelectric organic field-effect transistor (Fe-OFET) nonvolatile memory (NVM). In this work, we propose a novel route to resolve this issue by employing two ultrathin AlO interfacial layers sandwiching an ultrathin ferroelectric polymer film with a low coercive field, in the fabricated flexible Fe-OFET NVM. The operation voltage of Fe-OFET NVMs decreases with the downscaling thickness of the ferroelectric film. By inserting two ultrathin AlO interfacial layers at both sides of the ultrathin ferroelectric film, not only the gate leakage is prominently depressed but also the mobility is greatly improved. Excellent memory performances, with large mobility of 1.7 ~ 3.3 cm V s, high reliable memory switching endurance over 2700 cycles, high stable data storage retention capability over 8 × 10 s with memory on-off ratio larger than 10, are achieved at the low operating voltage of 4 V, which is the lowest value reported to data for all Fe-OFET NVMs. Simultaneously, outstanding mechanical fatigue property with the memory performances maintaining well over 7500 bending cycles at a bending radius of 5.5 mm is also achieved in our flexible FE-OFET NVM.
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http://dx.doi.org/10.1038/s41598-017-09533-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5566424PMC
August 2017

Co‑expression of Axin and APC gene fragments inhibits colorectal cancer cell growth via regulation of the Wnt signaling pathway.

Mol Med Rep 2017 Oct 21;16(4):3783-3790. Epub 2017 Jul 21.

Department of Oncology, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, P.R. China.

Adenomatous polyposis coli (APC) and Axin interactions serve an important role in colorectal cancer (CRC) pathogenesis. The aim of the present study was to assess the combined effects of Axin and APC co‑expression in CRC cells, and to determine the underlying mechanisms involved. SW480 cells were divided into the following groups: Untransfected (SW480 group), transfected with pEGFP‑N3plus pCS2‑MT (SW480/vector‑vector), transfected with pEGFP‑N3‑APC5 (SW480/APC5), and transfected with pEGFP‑N3‑APC5 pluspCS2‑MT‑Axin (SW480/APC5‑Axin). APC5 and Axin mRNA levels were determined by reverse transcription‑polymerase chain reaction. MTT assays and flow cytometry analysis were performed to assess cell growth and cell cycle distribution, respectively. Quantitative PCR and western blot analyses were conducted to evaluate the mRNA and protein levels, respectively, of Wnt signaling effectors, including β‑catenin, c‑myc and survivin. Successful transfection of SW480 cells was determined with APC and APC‑Axin plasmids as indicated by the green fluorescence signals. Notably, SW480/APC5 cell growth was inhibited by 40.33%, and cells co‑expressing APC5 and Axin demonstrated 61.27% inhibition of cell growth compared with SW480 control cells. The results demonstrate that APC5 may induce G1/S arrest in SW480 cells, and Axin may enhance cell growth arrest induced by APC5. The mRNA and protein levels of β‑catenin, c‑myc and survivin were significantly reduced in SW480/APC‑Axin cells when compared with the SW480/APC group. In conclusion, co‑expression of APC5 and Axin genes significantly downregulated Wnt signaling in human SW480 CRC cells and inhibited cell growth, when compared with cells transfected with APC5 alone. These results may provide experimental evidence to support combined gene therapy in CRC.
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http://dx.doi.org/10.3892/mmr.2017.7049DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5646956PMC
October 2017

Esophageal squamous cell carcinoma cell proliferation induced by exposure to low concentration of cigarette smoke extract is mediated via targeting miR-101-3p/COX-2 pathway.

Oncol Rep 2016 Jan 2;35(1):463-71. Epub 2015 Nov 2.

Department of Gastroenterology, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, P.R. China.

Cigarette smoke has been implicated as a major risk factor for esophageal squamous cell carcinoma (ESCC). Several lines of evidence have suggested that the promoting effect of cigarette smoking extract (CSE) on ESCC is mediated by upregulation of cyclooxygenase-2 (COX-2) expression. Yet, the underlying molecular and cellular mechanisms of how CSE stimulates COX-2 expression and facilitates ESCC development are largely unknown. In the present study, we revealed microRNA (miR)-101-3p expression was downregulated upon exposure to low concentration of CSE in Eca109 cancer cells, and suppression of miR-101-3p was required for low CSE-induced cell proliferation, presenting as overexpression of miR-101-3p reversing CSE stimulated cancer cell growth. Luciferase assay revealed that COX-2 was a direct target for miR-101-3p and overexpression of miR-101-3p decreased cellular COX-2 protein expression. Furthermore, we found that COX-2 inhibitor and knockdown of COX-2 by siRNA interference could abolish CSE-induced cell proliferation, indicating that promotion of cancer cell proliferation by low concentration of CSE was dependent on COX-2 activity. Finally, downregulation of miR-101-3p expression and upregulation of COX-2 was found in ESCC specimens from patients with smoking history. Taken together, our findings revealed a new post-transcriptional mechanism by which CSE regulated COX-2 expression to favor cancer cell proliferation, suggesting miR-101-3p as a potential biomarker and therapeutic target for smoke-related ESCC.
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http://dx.doi.org/10.3892/or.2015.4379DOI Listing
January 2016

Maximum temperature accounts for annual soil CO2 efflux in temperate forests of Northern China.

Sci Rep 2015 Jul 16;5:12142. Epub 2015 Jul 16.

1] Ministry of Education Key Laboratory for Silviculture and Conservation, Beijing Forestry University, Beijing, 100083, China [2] The Institute of Forestry and Climate Change Research, Beijing Forestry University, Beijing, 100083, China.

It will help understand the representation legality of soil temperature to explore the correlations of soil respiration with variant properties of soil temperature. Soil temperature at 10 cm depth was hourly logged through twelve months. Basing on the measured soil temperature, soil respiration at different temporal scales were calculated using empirical functions for temperate forests. On monthly scale, soil respiration significantly correlated with maximum, minimum, mean and accumulated effective soil temperatures. Annual soil respiration varied from 409 g C m(-2) in coniferous forest to 570 g C m(-2) in mixed forest and to 692 g C m(-2) in broadleaved forest, and was markedly explained by mean soil temperatures of the warmest day, July and summer, separately. These three soil temperatures reflected the maximum values on diurnal, monthly and annual scales. In accordance with their higher temperatures, summer soil respiration accounted for 51% of annual soil respiration across forest types, and broadleaved forest also had higher soil organic carbon content (SOC) and soil microbial biomass carbon content (SMBC), but a lower contribution of SMBC to SOC. This added proof to the findings that maximum soil temperature may accelerate the transformation of SOC to CO2-C via stimulating activities of soil microorganisms.
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http://dx.doi.org/10.1038/srep12142DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4503994PMC
July 2015

Effect of three common IL-17 single nucleotide polymorphisms on the risk of developing gastric cancer.

Oncol Lett 2015 Mar 23;9(3):1398-1402. Epub 2014 Dec 23.

Department of Vasculocardiology, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, P.R. China.

A 1:1 matched case-control study was conducted to analyze the association between three common interleukin (IL)-17A and IL-17F single nucleotide polymorphisms (SNPs) and the risk of developing gastric cancer. Genotyping of SNPs rs2275913, rs763780 and rs3748067 within the IL-17 gene were detected by performing polymerase chain reaction-restriction fragment length polymorphism analysis. Gastric cancer patients were more likely to be cigarette smokers, alcohol drinkers and have a family history of cancer in their first-degree relatives. Patients carrying the rs763780 polymorphism were correlated with a significant increased risk of gastric cancer in codominant, dominant and recessive models. Additionally, individuals with the rs763780 polymorphism were correlated with a markedly increased risk of gastric cancer among alcohol drinkers in codominant, dominant and recessive models. Furthermore, a significant correlation was identified between the rs763780 polymorphism and the consumption of alcohol. However, no association was identified between rs2275913 and rs3748067 polymorphisms and the risk of developing gastric cancer. Thus, the present study reported that the rs763780 polymorphism may be associated with risk of developing gastric cancer in the population studied, particularly in alcohol drinkers.
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http://dx.doi.org/10.3892/ol.2014.2827DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4314969PMC
March 2015

Bioavailability enhancement of osthole after oral administration of Bushen Yizhi prescription extract to rats followed by Cnidium monnieri (L.) Cusson fruits extract in comparison to pure osthole at different doses.

J Ethnopharmacol 2014 Mar 16;152(2):266-71. Epub 2013 Oct 16.

Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine, Guangzhou, China. Electronic address:

Ethnopharmacological Relevance: Bushen Yizhi prescription (BSYZ) is a traditional Chinese compound prescription, which is commonly used in China for treating ShenXu and hypophrenia based on traditional Chinese medicine and Alzheimer's Disease according to modern Chinese medicine. Cnidium monnieri (L.) Cusson fruits (CM) is treated as the main herb of BSYZ, and its main active ingredient Osthole (OST) is considered as one of the major active ingredients of BSYZ. Even though OST plays an important role in the BSYZ its bioavailability is poor. In order to investigate whether the bioavailability of OST was influenced by BSYZ and CM extract, the comparative evaluations on pharmacokinetics of OST after oral administration of pure OST at different doses, CM and BSYZ extract were studied.

Materials And Methods: 30 rats were randomly assigned to five groups and orally administered with pure OST at different doses (15, 75 and 150 mg/kg), CM (15 mg/kg OST) and BSYZ (15 mg/kg OST) extract. At different predetermined time points after administration, the concentrations of OST in rat plasma were determined by using the HPLC-UV method, and main pharmacokinetic parameters were investigated.

Results: The results showed that the pharmacokinetic parameters of OST were significantly different (p<0.05) among the groups. The AUC(0→t), AUC(0→∞) and Cmax of OST were significantly increased after oral administration of BSYZ extract, followed by CM extract, in comparison to pure osthole at different doses.

Conclusions: This present study indicated that the bioavailability of pure OST after oral administration was extremely low and it was dramatically enhanced because of the synergistic effect of the traditional Chinese Bushen Yizhi prescription.
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http://dx.doi.org/10.1016/j.jep.2013.09.045DOI Listing
March 2014

[Expression of thymic stromal lymphopoietin in nasal mucosa of a mouse model with allergic rhinitis].

Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2009 Sep;23(17):794-5, 799

Department of ICU, Zhongshan Hospital, Xiamen University, Xiamen, 361004, China.

Objective: To investigate the expression of thymic stromal lymphopoietin (TSLP) in the nasal mucosa of mouse with allergic rhinitis.

Method: Twenty wide type BALB/c mouse were divided into 2 groups randomly. Two groups were included, allergic rhinitis group (group A) and control group (group B). The mouse model of allergic rhinitis was established by ovalbumin (OVA) sensitization and challenge. The expressions of TSLP in the nasal mucosa was determined by realtime quantitative PCR and immunohistochemical method.

Result: The expression of TSLP in the nasal mucosa of group A was significantly higher than that in group B (P<0.01).

Conclusion: TSLP plays a role in the mouse model of allergic rhinitis.
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September 2009

[Nerve tissue morphological study of tutin microinjection into pontine NPBM in two hours].

Hua Xi Yi Ke Da Xue Xue Bao 2002 Jan;33(1):54-7

Department of Physiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.

Objective: To observe whether Tutin microinjection into the pontine NPBM respiratory area of rabbit will cause morphological damage to that area two hours later.

Methods: At two hours after the microinjection of Tutin into NPBM, the experimental effects on respiration came to be remarkable and the physiological condition was well, the rabbit was subjected to morphological sampling then. The sample was cut into slices for LM (Nissl dyeing) and transmission EM observation and photography.

Results: Under the LM and EM examination, no remarkable morphological damage done by Tutin microinjection into the pontine NPBM was observed. By comparing the Tutin-injected side with the other side of NPBM where equal normal saline microinjection was given, no apparent morphological difference could be found.

Conclusion: In our experiment condition, there was no morphological damage caused by Tutin microinjection into pontine NPBM respiratory area of the rabbit.
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January 2002
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