Publications by authors named "Meijun Zhou"

18 Publications

  • Page 1 of 1

The LPS induced pyroptosis exacerbates BMPR2 signaling deficiency to potentiate SLE-PAH.

FASEB J 2021 Dec;35(12):e22044

Institute of Basic Medical Sciences, School of Basic Medicine Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, China.

Pulmonary arterial hypertension (PAH) is a common and fatal complication of systemic lupus erythematosus (SLE). Whether the BMP receptor deficiency found in the genetic form of PAH is also involved in SLE-PAH patients remains to be identified. In this study, we employed patient-derived samples from SLE-associated PAH (SLE-PAH) and established comparable mouse models to clarify the role of BMP signaling in the pathobiology of SLE-PAH. Firstly, serum levels of LPS and autoantibodies (auto-Abs) directed at BMP receptors were significantly increased in patients with SLE-PAH compared with control subjects, measured by ELISA. Mass cytometry was applied to compare peripheral blood leukocyte phenotype in patients prior to and after treatment with steroids, which demonstrated inflammatory cells alteration in SLE-PAH. Furthermore, BMPR2 signaling and pyroptotic factors were examined in human pulmonary arterial endothelial cells (PAECs) in response to LPS stimulation. Interleukin-8 (IL-8) and E-selectin (SELE) expressions were up-regulated in autologous BMPR2 endothelial cells and siBMPR2-interfered PAECs. A SLE-PH model was established in mice induced with pristane and hypoxia. Moreover, the combination of endothelial specific BMPR2 knockout in SLE mice exacerbated pulmonary hypertension. Pyroptotic factors including gasdermin D (GSDMD) were elevated in the lungs of SLE-PH mice, and the pyroptotic effects of serum samples isolated from SLE-PAH patients on PAECs were analyzed. BMPR2 signaling upregulator (BUR1) showed anti-pyroptotic effects in SLE-PH mice and PAECs. Our results implied that deficiencies of BMPR2 signaling and proinflammatory factors together contribute to the development of PAH in SLE.
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http://dx.doi.org/10.1096/fj.202100851RRDOI Listing
December 2021

The value of the Demetics ultrasound-assisted diagnosis system in the differential diagnosis of benign from malignant thyroid nodules and analysis of the influencing factors.

Eur Radiol 2021 Oct 15;31(10):7936-7944. Epub 2021 Apr 15.

Department of Ultrasound, Institute of Ultrasound in Musculoskeletal Sports Medicine, Guangdong Second Provincial General Hospital, Guangzhou, 510317, Guangdong, People's Republic of China.

Objectives: To evaluate the value of Demetics and to explore whether Demetics can help radiologists with varying years of experience in the differential diagnosis of benign from malignant thyroid nodules.

Methods: The clinical application value of Demetics was assessed by comparing the diagnostic accuracy of radiologists before and after applying Demetics. This retrospective analysis included 284 thyroid nodules that underwent pathological examinations. Two different combined methods were applied. Using method 1: the original TI-RADS classification was forcibly upgraded or downgraded by one level when Demetics classified the thyroid nodules as malignant or benign. Using method 2: the TI-RADS and benign or malignant classification of the thyroid nodules were flexibly adjusted after the physician learned the Demetics' results.

Results: Demetics exhibited a higher sensitivity than did junior radiologist 1 (p = 0.029) and was similar in sensitivity to the two senior radiologists. Demetics had a higher AUC than both junior radiologists (p = 0.042, p = 0.038) and an AUC similar to that of the senior radiologists. The sensitivity (p = 0.035) and AUC (p = 0.031) of junior radiologist 1 and the specificity (p < 0.001) and AUC (p = 0.026) of junior radiologist 2 improved with combined method 1. The AUC of junior radiologist 2 improved with combined method 2 (p = 0.045). The factors influencing the diagnostic results of Demetics include sonographic signs (echogenicity and echogenic foci), contrast of the image, and nodule size.

Conclusion: Demetics exhibited high sensitivity and accuracy in the differential diagnosis of benign from malignant thyroid nodules. Demetics could improve the diagnostic accuracy of junior radiologists.

Key Points: • Demetics exhibited a high sensitivity and accuracy in the differential diagnosis of benign from malignant thyroid nodules. • Demetics could improve the diagnostic accuracy of junior radiologists in the differential diagnosis of benign from malignant thyroid nodules. • Factors influencing the diagnostic results of Demetics include the sonographic signs (echogenicity and echogenic foci), contrast of the image, and nodule size.
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http://dx.doi.org/10.1007/s00330-021-07884-zDOI Listing
October 2021

Risk factors for ineffective recanalization after endovascular treatment in acute ischemic stroke.

Clin Neurol Neurosurg 2021 01 11;200:106362. Epub 2020 Nov 11.

Department of Neurology, Xuanwu Hospital Capital Medical University, Beijing, China.

Objective: To assess the risk factors of early ineffective recanalization, defined as a modified Rankin scale (mRS) score of 4∼6 at two weeks after symptom onset, in acute ischemic stroke (AIS) patients receiving endovascular treatment (EVT).

Method: One hundred eighty-four AIS patients who received EVT in 01/2016-12/2018 were analyzed. The recanalization rate was 81.5 % (150/184). Patients were divided into effective (mRS 0-3) and ineffective (mRS 4-6) recanalization groups. The independent risk factors for ineffective recanalization were analyzed.

Result: The ineffective recanalization rate was 67.3 % (101/150 cases) at 14 days after symptom onset. Twenty-five patients (24.8 %) in the ineffective recanalization group had coronary heart disease, higher than that in the effective group (4 patients, 8.2 %, P = 0.016). Infratentorial pathology accounted for 34.7 % (35 cases) of the ineffective group and only 14.3 % (7 cases) of the control group (P = 0.011). Systolic blood pressure (SBP) was higher in the ineffective group than in the effective group (146 mmHg vs 140 mmHg, P = 0.038). General anesthesia was more common in the ineffective group than in the control group (49 cases, 48.5 %, vs 9 cases, 18.4 %; P = 0.000). Logistic regression showed that coronary heart disease (odds ratio (OR) = 3.399, 95 % confidence interval (CI) 1.013-11.401, P = 0.048), unconsciousness before EVT (OR = 4.292, 95 % CI 1.963-9.386, P = 0.000), SBP (OR = 1.016, 95 % CI 0.997-1.035, P = 0.090) and general anesthesia (OR = 3.378, 95 % CI 1.395-8.180, P = 0.007) were independent risk factors for ineffective recanalization.

Conclusion: Patients with coronary heart disease, unconsciousness before EVT, elevated SBP and general anesthesia need precise assessment to benefit from EVT.
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http://dx.doi.org/10.1016/j.clineuro.2020.106362DOI Listing
January 2021

Transvaginal four-dimensional hysterosalpingo-contrast sonography: Pain perception and factors influencing pain severity.

J Obstet Gynaecol Res 2021 Jan 26;47(1):302-310. Epub 2020 Oct 26.

Department of Ultrasonography, Institute of Ultrasound in Musculoskeletal Sports Medicine, Guangdong Second Provincial General Hospital, Guangzhou, China.

Aim: The purpose is to investigate pain perception during transvaginal four-dimensional hysterosalpingo-contrast sonography (TV 4D-HyCoSy) and factors influencing pain severity.

Methods: This was a retrospective study included 340 women who underwent TV 4D-HyCoSy examination from January 2016 to October 2017. The factors were recorded, including age, childbearing history, infertility type, history of pelvic inflammation, pelvic surgery, history of uterine manipulation, history of ectopic pregnancy, atropine delivery mode, uterine position, uterine malformation, uterine lesion, fibroid, intrauterine adhesion, polycystic ovary, endometrial implantation cyst, dysmenorrhea score, the degree of patency of fallopian tube and contrast agents dosage. Pain was evaluated during and after TV 4D-HyCoSy. The time point of peak pain was evaluated and the influencing factors of moderate/severe pain were analyzed.

Results: The highest pain occurred at contrast instillation. The independent influencing factors of moderate/severe pain were age (P = 0.021), dysmenorrhea score (P = 0.003) and tubal patency (P < 0.001). Further analysis showed that age affected the pain score when TV 4D-HyCoSy started and the peak pain occurred. Dysmenorrhea score and tubal patency affect the pain score at most time points.

Conclusion: Age, dysmenorrhea score and tubal patency are factors influencing the severity of pain during TV 4D-HyCoSy.
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http://dx.doi.org/10.1111/jog.14538DOI Listing
January 2021

Spontaneous conception outcome in infertile women after four-dimensional hysterosalpingo-contrast-sonography.

BMC Pregnancy Childbirth 2020 Oct 20;20(1):638. Epub 2020 Oct 20.

Department of Ultrasound, Institute of Ultrasound in Musculoskeletal Sports Medicine, Guangdong Second Provincial General Hospital, Guangzhou, 510317, Guangdong, PR China.

Background: Four-dimensional hysterosalpingo-contrast sonography (4D-HyCoSy) is the preferred way for evaluating fallopian tubal patency and it associated with higher rate of spontaneous conception. However, Few studies have evaluated the influencing factors of spontaneous conception in 4D-HyCoSy and suggested ways to choose treatment options after 4D-HyCoSy. The study was to evaluate the correlation between spontaneous conception outcome and the patients' clinical characteristics as well as tubal patency in infertile women to provide reference on ways to manage the patient after 4D-HyCoSy.

Methods: This was a retrospective study and analysis of two hundred and eighty three (283) infertile patients who underwent a 4D-HyCoSy between December 2014 and October 2017 in our center. Eligible patients were those whose partners semen parameters were normal when based on World Health Organization (WHO) criteria, and had spontaneous conception without clinical interventions after 4D-HyCoSy.

Result(s): One hundred and sixteen patients (40.9%) conceived spontaneously and the mean conception time was (8.8 ± 0.3) months. Within a year after 4D-HyCoSy, the spontaneous conception rate was highest in type VI(62.5%), followed by type IV (46.2%), type III (44.4%), type V (39.4%), type II (33.9%) and type I (14.8%). With Cox regression analysis, two factors associated with spontaneous conception outcome appeared to increase spontaneous conception rate: patients with type IV or type VI tubes and duration of infertility less than 2 years. The age, type of infertility, multiparas, history of pelvic surgery, history of uterine cavity operation, uterine fibromyomata and polycystic ovary were unrelated to spontaneous conception outcome after 4D-HyCoSy.

Conclusion(s): This study showed that some infertile women could succeed in spontaneous conception after 4D-HyCoSy. Hence, We recommend the usage of 4D-HyCoSy as first line for tubal patency test and infertile patients should be advised to accept 4D-HyCoSy examination as soon as possible. Expectant treatment of about 8-9 months is reported to be feasible for infertile women whose 4D-HyCoSy findings showed one tube patency or poor patency. Alternatively, an immediate clinical intervention is recommended for those with bilateral obstructed tubes .
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http://dx.doi.org/10.1186/s12884-020-03315-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7574424PMC
October 2020

Palliative Radiofrequency Ablation Accelerates the Residual Tumor Progression Through Increasing Tumor-Infiltrating MDSCs and Reducing T-Cell-Mediated Anti-Tumor Immune Responses in Animal Model.

Front Oncol 2020 4;10:1308. Epub 2020 Sep 4.

Department of Ultrasound, Institute of Ultrasound in Musculoskeletal Sports Medicine, Guangdong Second Provincial General Hospital, Southern Medical University, Guangzhou, China.

Previous studies showed that radiofrequency ablation (RFA) has a favorable treatment efficacy for hepatocellular carcinoma (HCC) or colorectal liver metastases (CRLMs). Palliative RFA (pRFA) resulting from larger HCC or multiple CRLMs further accelerated the progression of potential residual tumor, yet its mechanism was still unknown. This study investigated the influence of myeloid-derived suppressor cells (MDSCs) on T-cell immune responses and tumor recurrence after pRFA. CT26 tumor models were used. The percentage of MDSCs in peripheral blood was analyzed by flow cytometry after pRFA. The level of Th1 and Th2 cytokines were measured by ELISA through different treatments ( = 4/group). The tumor-infiltrating MDSCs, dendritic cells, and intracellular cytokines level were analyzed by IHC staining after different treatments. The functional CD8 T cells were confirmed by the co-localization immunofluorescence staining. The long-term outcomes were also evaluated through CT26 and 4T1 tumor models. The results showed that tumor models treated with pRFA displayed significant increases in the percentage of MDSCs of peripheral blood and tumor infiltration. The expression level of TGF-β and IL-6 after pRFA was higher than that before pRFA by ELISA and IHC staining. After depleting MDSCs by combining with Abs, the pRFA + Abs group achieved a higher level of Th1 cytokines and greatly enhanced the percentage of tumor-infiltrating functional CD8 T cells when compared with pRFA alone. The depletion of MDSCs through combination with Abs also resulted in tumor regression. In conclusion, pRFA accelerates the residual tumor progression through increasing tumor-infiltrating MDSCs and reducing T-cell-mediated anti-tumor immune responses, which could provide a potential approach for delaying tumor recurrence caused by pRFA.
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http://dx.doi.org/10.3389/fonc.2020.01308DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7498645PMC
September 2020

Near-infrared fluorescence imaging-guided focused ultrasound-mediated therapy against Rheumatoid Arthritis by MTX-ICG-loaded iRGD-modified echogenic liposomes.

Theranostics 2020 8;10(22):10092-10105. Epub 2020 Aug 8.

The Second School of Clinical Medicine, Southern Medical University, Guangzhou 510515, China.

Rheumatoid arthritis (RA), a common inflammatory disorder of the joints characterized by synovitis and pannus formation, often results in irreversible joint erosion and disability. Methotrexate (MTX) is the first-line drug against RA, but the therapeutic effects are sub-optimal due to its poor retention at the target sites and systemic side effects. Multifunctional nanoparticles are highly promising agents for minimally invasive, traceable and effective targeted therapy. This study developed iRGD peptide-functionalized echogenic liposomes (iELPs) which encapsulates MTX and indocyanine green (ICG) fluorescent probe through the thin film-hydration method. The resulting iELPs showed high affinity for endothelial cells overexpressing αvβ3 integrin, favorable acoustic response and fluorescence tracking properties. Also, near-infrared (NIR) fluorescence imaging of iELPs and ultrasound-triggered drug release of MTX were proved in a mouse RA model, greatly improving the therapeutic efficacy and reducing MTX side effects. Histological assessment of the articular tissues further revealed significantly lower inflammatory cell infiltration and angiogenesis in the iELPs-treated and sonicated mice. Our study provides a promising nanoplatform for integrating ultrasound-controlled drug release and NIR fluorescence imaging for RA treatment.
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http://dx.doi.org/10.7150/thno.44865DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7481417PMC
May 2021

Whole-Mount In Situ Hybridization in Zebrafish Embryos and Tube Formation Assay in iPSC-ECs to Study the Role of Endoglin in Vascular Development.

J Vis Exp 2020 05 28(159). Epub 2020 May 28.

Department of Physiology and Department of Cardiology of the Second Affiliated Hospital, Zhejiang University School of Medicine; Department of Cell Biology, State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College;

Vascular development is determined by the sequential expression of specific genes, which can be studied by performing in situ hybridization assays in zebrafish during different developmental stages. To investigate the role of endoglin(eng) in vessel formation during the development of hereditary hemorrhagic telangiectasia (HHT), morpholino-mediated targeted knockdown of eng in zebrafish are used to study its temporal expression and associated functions. Here, whole-mount in situ RNA hybridization (WISH) is employed for the analysis of eng and its downstream genes in zebrafish embryos. Also, tube formation assays are performed in HHT patient-derived induced pluripotent stem cell-differentiated endothelial cells (iPSC-ECs; with eng mutations). A specific signal amplifying system using the whole amount In Situ Hybridization - WISH provides higher resolution and lower background results compared to traditional methods. To obtain a better signal, the post-fixation time is adjusted to 30 min after probe hybridization. Because fluorescence staining is not sensitive in zebrafish embryos, it is replaced with diaminobezidine (DAB) staining here. In this protocol, HHT patient-derived iPSC lines containing an eng mutation are differentiated into endothelial cells. After coating a plate with basement membrane matrix for 30 min at 37 °C, iPSC-ECs are seeded as a monolayer into wells and kept at 37 °C for 3 h. Then, the tube length and number of branches are calculated using microscopic images. Thus, with this improved WISH protocol, it is shown that reduced eng expression affects endothelial progenitor formation in zebrafish embryos. This is further confirmed by tube formation assays using iPSC-ECs derived from a patient with HHT. These assays confirm the role for eng in early vascular development.
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http://dx.doi.org/10.3791/60498DOI Listing
May 2020

Evidence of Accumulated Endothelial Progenitor Cells in the Lungs of Rats with Pulmonary Arterial Hypertension by Zr-oxine PET Imaging.

Mol Ther Methods Clin Dev 2020 Jun 3;17:1108-1117. Epub 2020 May 3.

State Key Laboratory of Medical Molecular Biology, Department of Cell Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College, Beijing 100730, China.

Endothelial progenitor cells (EPCs) play a major role in regulating pulmonary vascular remodeling during pulmonary arterial hypertension (PAH) development. Several preclinical and clinical trials of EPCs transplantation have been performed for the treatment of PAH. However, there is no reliable method to monitor real-time cell trafficking and quantify transplanted EPCs. Here in this paper we isolated EPCs from human peripheral blood, identified their functional integrity, and efficiently labeled the EPCs with Zr-oxine and DiO. Labeled EPCs were injected into the tail vein of normal and PAH rats to be tracked . From the microPET/CT images, we found EPCs were distributed primarily in the lung at 1 h and then migrated to the liver and spleen. We could observe the 3,3' dioctadecyloxacarbocyanine perchlorate (DiO)-labeled EPCs binding in the pulmonary vasculature by CellVizio confocal. The result of quantitative analysis revealed significantly higher accumulation of EPCs in the lungs of PAH rats than in those of healthy rats. The distribution and higher accumulation of EPCs in the lungs of PAH rats could help to evaluate the safety and provide evidence of effectiveness of EPC therapy.
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http://dx.doi.org/10.1016/j.omtm.2020.04.021DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7256434PMC
June 2020

Climate change in different geographical units and its impact on land production potential: a case study of Shaanxi Province, China.

Environ Sci Pollut Res Int 2019 Aug 31;26(22):22273-22283. Epub 2019 May 31.

College of Urban and Environmental Science, Northwest University, No. 1 Xuefu Avenue, Xi'an, Shaanxi, China.

Land production potential (LPP) was the maximum grain yield in one year that can be produced by land under the limitations of climate conditions and in the absence of pests and diseases and other factors. Whether climate change was increasing or reducing the LPP in a given region was uncertain. Therefore, Shaanxi Province was selected to analyze the regional differences in climate change and its effects on LPP change and to identify the main climatic factor restricting LPP in different regions by combining Global Agro-Ecological Zone (GAEZ) model with the Geodetector model. Results showed that the temperature in Shaanxi Province showed an upward trend in 2000-2015; the rise in temperature to the north of Qinling Mountain (QM) was less than that to the south of QM. However, rising temperature had a yield-improving effect to the north of QM and a yield-decreasing effect to the south of QM. There was a precipitation increase in Arid Sandy (AS) area and Loess Plateau (LP), and the precipitation reduced in all other geographical units. The increase in LPP of Shaanxi mostly was caused by increasing precipitation. However, precipitation was declined and reduced LPP to the south of QM; that is, precipitation decline was the dominated climatic factor for LPP decrease in QM, Hanjiang Basin (HB), and Daba Mountain (DM). To the north of QM, LPP in AS, LP, and Guanzhong Plain (GP) both dramatically increased, mainly improved by rising temperature, increasing precipitation, and rising temperature, respectively.
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http://dx.doi.org/10.1007/s11356-019-05503-5DOI Listing
August 2019

Theranostic nanosensitizers for highly efficient MR/fluorescence imaging-guided sonodynamic therapy of gliomas.

J Cell Mol Med 2018 11 29;22(11):5394-5405. Epub 2018 Aug 29.

Department of Ultrasonography, Guangdong Second Provincial General Hospital, Guangzhou, China.

Glioma is the most frequent primary brain tumour of the central nervous system. Its high aggressiveness and deep-seated brain lesion make it a great challenge to develop a non-invasive, precise and effective treatment approach. Here, we report a multifunctional theranostic agent that can integrate imaging and therapy into a single nano-platform for imaging-guided sonodynamic therapy (SDT). The SDT agents were fabricated by encapsulation of sinoporphyrin sodium (DVDMS) chelating with manganese ions into nanoliposomes (DVDMS-Mn-LPs). DVDMS-Mn-LPs are physiologically stable and biologically compatible, and they can produce singlet oxygen upon ultrasound irradiation to kill cancer cells. Both cell and animal studies demonstrated that SDT with DVDMS-Mn-LPs can significantly improve the antitumour growth efficiency even in the presence of skull. In addition, DVDMS-Mn-LPs are good for MR and fluorescence imaging. Thus, DVDMS-Mn-LPs reported here may provide a promising strategy for imaging-guided modality for glioma treatment.
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http://dx.doi.org/10.1111/jcmm.13811DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6201228PMC
November 2018

Fast preparation of carbon spheres from enzymatic hydrolysis lignin: Effects of hydrothermal carbonization conditions.

Sci Rep 2018 06 22;8(1):9501. Epub 2018 Jun 22.

College of Materials Science and Engineering, Nanjing Forestry University, Nanjing, 210037, China.

This study explores the effect of carbon sphere preparation conditions on the morphology of the carbon spheres and the micropore development by fast potassium hydroxide activation via microwave heating. Enzymatic hydrolysis lignin is used as the precursor for carbon sphere preparation via environmentally friendly hydrothermal carbonization. The effects of various carbonization temperatures, carbonization times and reaction concentrations on the physical morphology of the carbon sphere surfaces are investigated. The Brunauer-Emmett-Teller surface area, yield and scanning electron microscopic images are used to characterize the carbon spheres. High carbonization temperatures and times result in large particle sizes, high sphericity, uniform size, and high dispersity of the carbon spheres. The best carbon spheres are obtained at 270 °C for 7 hours with a reaction concentration of 0.06 g ml and a particle size of 3-6 μm. After activation, the Brunauer-Emmett-Teller surface area of the activated carbon spheres increases from 248 m g to 1278 m g. Carbon spheres activated by treatment with fast potassium hydroxide and microwave heating can develop micropores that enhance the adsorptive capacity for small molecules, such as gases. Enzymatic hydrolysis lignin-derived carbon spheres formed via hydrothermal carbonization should be potentially sustainable materials applicable in energy and environmental fields.
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http://dx.doi.org/10.1038/s41598-018-27777-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6014991PMC
June 2018

Synovitis in mice with inflammatory arthritis monitored with quantitative analysis of dynamic contrast-enhanced NIR fluorescence imaging using iRGD-targeted liposomes as fluorescence probes.

Int J Nanomedicine 2018 23;13:1841-1850. Epub 2018 Mar 23.

Department of Ultrasonography, Guangdong Second Provincial General Hospital Affiliated to Southern Medical University, Guangzhou, China.

Background: Rheumatoid arthritis (RA) is a common inflammatory disorder characterized primarily by synovitis and pannus formation in multiple joints, causing joints destruction and irreversible disability in most cases. Early diagnosis and effective therapy monitoring of RA are of importance for achieving the favorable prognosis.

Methods: We first prepared the targeted fluorescence probes, and then explored the feasibility of near-infrared (NIR) fluorescence molecular imaging to detect and evaluate the RA via the targeted fluorescence probes by quantitative analysis in this study.

Results: The targeted fluorescence probes (indocyanine green-liposomes decorated with iRGD peptide [iLPs]) was successfully prepared. The quantitative analysis found that strong fluorescence signal was detected in inflamed paws and the fluorescence signal in iLPs group was 3.03-fold higher than that in non-targeted (indocyanine green-liposomes decorated without iRGD peptide [LPs]) group (<0.01) at 15 min after injection, whereas the fluorescence signal from iLPs signal can almost not be observed in the non-inflamed paws, showing the high sensitivity and accuracy for arthritis by the NIR fluorescence imaging based on iLPs.

Conclusion: The NIR fluorescence imaging by iLPs may facilitate improved arthritis diagnosis and early assessment of the disease progression by providing an in vivo characterization of angiogenesis in inflammatory joint diseases.
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http://dx.doi.org/10.2147/IJN.S155475DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5870656PMC
May 2018

Preparation and characterization of a novel silicon-modified nanobubble.

PLoS One 2017 30;12(5):e0178031. Epub 2017 May 30.

Paul C. Lauterbur Research Center for Biomedical Imaging, Institute of biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.

Nanobubbles (NBs) opened a new field of ultrasound imaging. There is still no practical method to control the diameter of bubbles. In this study, we developed a new method to control the size by incorporating of silicon hybrid lipids into the bubble membrane. The range of particle size of resulting NBs is between 523.02 ± 46.45 to 857.18 ± 82.90, smaller than the conventional microbubbles. The size of resulting NBs increased with the decrease in amount of silicon hybrid lipids, indicating the diameter of NBs can be regulated through modulating the ratio of silicon hybrid lipids in the bubble shell. Typical harmonic signals could be detected. The in vitro and in vivo ultrasound imaging experiments demonstrated these silicon-modified NBs had significantly improved ultrasound contrast enhancement abilities. Cytotoxicity assays revealed that these NBs had no obvious cytotoxicity to the 293 cell line at the tested bubble concentration. Our results showed that the novel NBs could use as nanoscale ultrasound contrast agents, providing the foundation for NBs in future applications including contrast-enhanced imaging and drug/gene delivery.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0178031PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5448765PMC
September 2017

Synthesis of poly(methacrylic acid)-manganese oxide dihydroxide/silica core-shell and the corresponding hollow microspheres.

J Colloid Interface Sci 2015 Jan 8;438:269-276. Epub 2014 Oct 8.

Key Laboratory of Functional Polymer Materials, Ministry of Education, Institute of Polymer Chemistry, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University, Tianjin 300071, PR China. Electronic address:

Poly(methacrylic acid)-MnO(OH)2/SiO2 core-shell microspheres were prepared by sol-gel hydrolysis of tetraethylorthosilicate (TEOS) in the presence of poly(methacrylic acid)-Mn(II) (PMAA-Mn(2+)) as template with ammonium hydroxide anion as catalyst and n-octadecyltrimethoxysilane (C18TMS) as pore-directing reagent. The PMAA-Mn(2+) core was prepared by incubation of Mn(2+) cations with PMAA microspheres via the coordination between carboxylate anion group on PMAA microsphere and Mn(2+) cations. During this process, the Mn(II) species were formed as white Mn(OH)2 precipitates at first, which were subsequently oxidized into brown MnO(OH)2 in air. The Mn2O3/mesoporous silica (Mn2O3/m-SiO2) double-shelled hollow microspheres (DSHMs) were prepared through calcination of the PMAA-MnO(OH)2/SiO2 core-shell microspheres at 600 °C for the selective removal of PMAA template and pore-directing organic component from C18TMS, during which the crystalline structure of DSHM was developed into Braunite-1Q via the reaction between Mn2O3 inner-shell and silica outer-shell by annealing the DSHMs under higher temperatures of 800 and 900 °C. The Mn2O3 hollow microspheres (HMs) were prepared through the selective removal of the silica layer from the DSHMs by sodium hydroxide aqueous solution, which exhibited structure integrity and good ethanol dispersity due to the presence of mesoporous structure.
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http://dx.doi.org/10.1016/j.jcis.2014.09.051DOI Listing
January 2015

The effect of small dose sufentanil on emergence agitation in preschool children following sevoflurane anesthesia for elective repair of unilateral inguinal hernia.

Saudi Med J 2013 Jan;34(1):40-5

Department of Anesthesiology, Mianyang Central Hospital, 12 Changjia Lane, Mianyang, Sichuan, China.

Objective: To conduct a comparative study of sufentanil and fentanyl on emergence agitation in pediatric patients.

Methods: We conducted a prospective, randomized, and double-blind study in 80 preschool children admitted at the Pediatric Surgery, Mianyang Central Hospital, Sichuan, China between March 2011 and April 2012. They underwent sevoflurane anesthesia for elective repair of unilateral inguinal hernia. Children received a single intravenous dose of sufentanil 0.15 ug/kg or fentanyl 1.5 ug/kg just before skin incision. The emergence agitation scale and the frequency of severe emergence agitation were measured. Patients who required additional fentanyl during surgery and postoperative rescue fentanyl were recorded. Recovery time and the incidence of adverse effects were assessed.

Results: The mean emergence agitation scores were significantly lower in the sufentanil group compared with the fentanyl group (9.1+/-3.5 versus 12+/-3.8; 95% confidence interval [1.27+/-0.53]; p=0.001). The frequency of severe emergence agitation was 27.5% in the sufentanil group and 50% in the fentanyl group (p=0.039). Significantly fewer children in the sufentanil group received additional fentanyl during surgery compared to the fentanyl group (5% versus 20%; p=0.043) and significantly fewer children in the sufentanil group received one dose of postoperative rescue fentanyl compared to the fentanyl group (17.5% versus 42.5%; p=0.015). The incidence of vomiting was significantly higher in the fentanyl group than in the sufentanil group (p=0.023).

Conclusion: In preschool children undergoing repair of unilateral inguinal hernia with sevoflurane anesthesia, compared with a single dose of 1.5 ug/kg fentanyl, 0.15 ug/kg sufentanil before skin incision can significantly decrease the incidence of emergence agitation without delaying the recovery time.
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January 2013

Combination effects of chronic cadmium exposure and gamma-irradiation on the genotoxicity and cytotoxicity of peripheral blood lymphocytes and bone marrow cells in rats.

Mutat Res 2012 Mar 9;743(1-2):67-74. Epub 2012 Jan 9.

Department of Radiation Biology, Institute of Radiation Medicine, Fudan University, Shanghai, PR China.

This work investigated the effects of chronic cadmium (Cd) exposure combined with γ-ray irradiation on the cytotoxicity and genotoxicity of peripheral blood cells and bone marrow cells in rats. Results showed that when the rats were exposed to low dose (LD) Cd of 0.1mg CdCl₂/(kgd) for 8 and 12 weeks, the Cd concentration in blood reached to 135-140 μg/L and no toxic effects on peripheral blood lymphocytes, white blood cells (WBC) and granulocyte-monocyte (GM) progenitor cells were observed except polychromatic erythrocytes (PCE) of bone marrow. Moreover, this chronic LD Cd exposure significantly decreased irradiation-induced micronucleus (MN) formation and hypoxanthine-guanine phosphoribosyl transferase (hprt) mutation in lymphocytes and PCE, while the combination of LD Cd exposure and irradiation induced the additive metallothionein (MT) protein expression in bone marrow cells. When the rats were exposed to a high dose (HD) Cd of 0.5mg CdCl/₂(kgd) for 8 and 12 weeks, the blood Cd level approached to 458-613 μg/L and an inflammatory response was induced, meanwhile, MN formation and hprt mutation were markedly increased, and the ratio of PCE/NCE (normochromatic erythrocyte) was significantly decreased. Furthermore, when the rats were exposed to HD Cd plus 2 Gy irradiation, additive toxic effects on MN formation, hprt mutation, PCE damage and GM progenitor cell proliferation were observed, while this combination treatment resulted in an obvious reduction of MT protein compared to HD Cd group. In conclusion, chronic exposure to LD Cd induced the adaptive response to irradiation in the genotoxicity of peripheral blood lymphocytes and PCE of bone marrow by the up-regulation of Cd-induced MT protein, but the combination of HD Cd exposure and irradiation generated the additive effects on the cytotoxicity and genotoxicity in peripheral blood lymphocytes and bone marrow cells.
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http://dx.doi.org/10.1016/j.mrgentox.2011.12.025DOI Listing
March 2012

The role of nucleophosmin/B23 in radiation-induced chromosomal instability in human lymphoblastoid cells of different p53 genotypes.

Int J Radiat Biol 2010 Dec 1;86(12):1031-43. Epub 2010 Sep 1.

Department of Radiation Biology, Institute of Radiation Medicine, Fudan University, Shanghai, PR China.

Purpose: To investigate the role of nucleophosmin (NPM/B23) in radiation-induced chromosomal instability and apoptosis in human lymphoblastoid cells with different protein 53 (p53) status.

Materials And Methods: Wild type (wt) p53 TK6 and mutant type (mt) p53 WTK1 with or without short hairpin RNA (shRNA)-mediated silencing of NPM, TK6 with or without short interfering RNA (siRNA)-mediated silencing of p53 (p53i and NEGi) were irradiated with 4 Gy gamma-rays. Six to 48 h after irradiation, the index of apoptosis, chromosome aberration, cell cycle distribution and the levels of total NPM and phosphorylated-threonine 199 (pThr¹⁹⁹) NPM proteins were measured. Cells in some dishes were treated with 10 μM Olomoucine (OLO) for 3 h before irradiation and remained in the medium after irradiation.

Results: The rates of radiation-induced apoptosis in TK6 and TK6/NEGi were about 2-fold of those in WTK1 and TK6/p53i, while the frequencies of polyploidy in TK6 and TK6/NEGi were obviously lower than those in WTK1 and TK6/p53i. Moreover, after irradiation, pThr¹⁹⁹ NPM levels increased significantly in WTK1 and TK6/p53i, and slightly increased in TK6 and TK6/NEGi, indicating that the increased level of pThr¹⁹⁹ NPM was related to p53 status. When Thr¹⁹⁹ hyperphosphorylation of NPM was inhibited by OLO or when NPM was knocked down, we found that radiation-induced apoptosis was more pronounced and polyploidy formation was reduced as compared with negative control while the magnitude of these changes in TK6 was obviously higher than that in WTK1, indicating that NPM has an antagonistic interaction with wt p53.

Conclusions: NPM/B23 plays an important role in protecting cells from radiation-induced apoptosis and increasing polyploidy formation via either a p53 or non-p53 pathway.
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http://dx.doi.org/10.3109/09553002.2010.501843DOI Listing
December 2010
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